CN101805410A - Technological method for comprehensively producing pueraria flavonid, puerarin and arrowroot starch - Google Patents
Technological method for comprehensively producing pueraria flavonid, puerarin and arrowroot starch Download PDFInfo
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- CN101805410A CN101805410A CN 201010136218 CN201010136218A CN101805410A CN 101805410 A CN101805410 A CN 101805410A CN 201010136218 CN201010136218 CN 201010136218 CN 201010136218 A CN201010136218 A CN 201010136218A CN 101805410 A CN101805410 A CN 101805410A
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Abstract
The invention provides a technological method for comprehensively producing pueraria flavonid, puerarin and arrowroot starch, which belongs to the field of extracting effective ingredients from plants, and comprises the following steps: adopting a 80% ethanol microwave assisted extraction technology to obtain a pueraria flavonid solution and filter residue; adopting a macroporous resin adsorption method (AB-8 resin) to purify the pueraria flavonid solution and obtain the pueraria flavonid and puerarin products; and soaking the extracted filter residue by water, and separating the precipitation to obtain the arrowroot starch. The invention realizes the purpose of synchronously extracting the pueraria flavonid, the puerarin and the arrowroot starch from the radix puerariae, and effectively improves the product extraction rate.
Description
Technical field
The present invention relates to a kind of working method of from plant, extracting active substance, the processing method of a kind of specifically comprehensive production Radix Puerariae total flavones, puerarin and pueraria starch.
Background technology
The root of kudzu vine be traditional Chinese medicine be again protective foods.In addition, the root of kudzu vine China distribute wide, output is high, resource is very abundant, and Pueraria lobota is the rank vegetation, is suitable for establishing in large scale, is can sustainable development and the plant resources of utilization.But for a long time, the root of kudzu vine only is used as the raw material that extracts pueraria starch, and its processing mode also is the ten minutes primary, causes the significant wastage of resource.The main active chemical of the root of kudzu vine is Radix Puerariae total flavones and puerarin, at present Radix Puerariae total flavones and puerarin at strengthening immunity, prevent and treat aspects such as cardiovascular disorder, lowering blood glucose and have unusual effect.Be widely used in fields such as protective foods (complication that be used to relieve the effect of alcohol, chest enlarge, delaying female climacterium, diabetes causes etc.) simultaneously.In addition, low and significant biological activity also may be used on the health-care food aspect, and is used as the natural additive of food, makeup, as sweeting agent, antioxidant, food dye etc. because of its toxicity.
Along with the development of medical science, its pharmacological function and clinical treatment effect are familiar with by people gradually, and domestic and international market is also very vigorous to the demand of Radix Puerariae total flavones and puerarin at present.Therefore, the technical study of the extraction of active compound and separation and purification in the root of kudzu vine to improving the utility value of root of kudzu vine resource, obtains high economic benefit, has very significant meaning.How to extract and separate the big problem that the Radix Puerariae total flavones that obtains better quality and puerarin are current research.
Radix Puerariae total flavones and puerarin extraction and separation method are numerous, but much also there is defective, for example, in the domestic production at present, extract the technology that the Radix Puerariae flavone compounds mainly adopts traditional alcohol extracting, lixiviate, lead salt precipitation, used a large amount of organic solvents more, required energy consumption is big, time is long, and efficient is low.Some method has been abandoned the utilization of pueraria starch etc. in addition, can extract pueraria starch, Radix Puerariae total flavones and puerarin continuously though also there is individual processes to mention, and efficient is not high yet.These have all caused the waste of resource and the pollution of environment, and economic benefit is also lower simultaneously.At these problems, development new technologies, expansion advantage process application, process integration research explores that cost is low, efficient is high, can separate the novel process that obtains multiple root of kudzu vine effective ingredient again, just becomes current root of kudzu vine effective ingredient and extracts and isolating important research developing direction.
Summary of the invention
The objective of the invention is in order to overcome the weak point of above-mentioned background technology, the processing method of a kind of comprehensive production Radix Puerariae total flavones, puerarin and pueraria starch is provided, realize the purpose of simultaneous extraction Radix Puerariae total flavones, puerarin and pueraria starch from the root of kudzu vine, and can effectively improve the product extraction yield.
The processing method of a kind of comprehensive production Radix Puerariae total flavones provided by the invention, puerarin and pueraria starch may further comprise the steps:
(1) the fresh root of kudzu vine is cleaned section, carry out drying, crushing screening is weighed;
(2) will carry out wettingly through the above-mentioned pretreated root of kudzu vine, and under normal pressure, adopt 80% ethanol microwave auxiliary extraction, cold filtration promptly obtains Radix Puerariae total flavones extraction liquid and filter residue;
(3) the Radix Puerariae total flavones extraction liquid is concentrated, promptly obtain spissated Radix Puerariae total flavones solution;
(4-1) to AB-8 resin columns in series absorption on the spissated Radix Puerariae total flavones solution, the distilled water wash-out, 70% ethanol elution is collected elutriant, concentrates, and the vacuum decompression drying promptly obtains the Radix Puerariae total flavones product;
(4-2) to AB-8 resin columns in series absorption on the spissated Radix Puerariae total flavones solution, the distilled water wash-out, be washed to colourless after, 70% ethanol elution is collected elutriant 50ml to 100ml, concentrates, the vacuum decompression drying promptly obtains puerarin;
(5) gained filter residue in the step (2) is soaked, after twice of 180 mesh sieves filtration,, promptly obtained pueraria starch after the precipitation drying the centrifugation of gained filtrate.
In technique scheme, to carry out wetting through the above-mentioned pretreated root of kudzu vine described in the step (2), adopting 80% ethanol microwave auxiliary extraction operation specifically is that to get granularity earlier be the 80 purpose roots of kudzu vine, pre-bubble 1 hour, its solid-to-liquid ratio is 1g: 25ml, adopting power again is that the microwave of 800W is to wetting root of kudzu vine intermittent radiation 3 times, each radiation 40 seconds, adopting concentration is that 80% alcohol solvent is to wetting root of kudzu vine extraction, 80 ℃ of extraction temperature, 60 minutes extraction time.
In technique scheme, the concentration operation described in the step (3) specifically is first evaporating solvent, uses 95% ethanol sedimentation water-soluble impurity, evaporating solvent once more again.
In technique scheme, spissated Radix Puerariae total flavones strength of solution is 6.6931mg/ml in the last AB-8 resin columns in series adsorption operations described in step (4-1) and the step (4-2), and the sample solution amount is 3 times of AB-8 resin columns in series volumes.
The invention provides the processing method of a kind of comprehensive production Radix Puerariae total flavones, puerarin and pueraria starch, it adopts technology such as microwave auxiliary extraction technology, macroreticular resin absorbing method (AB-8 resin), vacuum decompression drying, realize the purpose of simultaneous extraction Radix Puerariae total flavones, puerarin and pueraria starch from the root of kudzu vine, and effectively improved the product extraction yield.This processing method compared with prior art has the following advantages:
1. change single extraction kudzu vine root in the present technology, caused the problem that wastage of material is big, benefit is low, can realize the continuous production of Radix Puerariae total flavones, puerarin and pueraria starch;
2. adopt innoxious solvent ethanol, guarantee that extraction process operation and the finished product are nontoxic;
3. adopt the microwave auxiliary extraction technology, the operating time is short, solvent-oil ratio is few, effective component yield is high;
4. adopt macroreticular resin absorbing method efficient height, be not subjected to inorganics to influence, regenerate to make things convenient for, technology is simple, production cost is low.
Description of drawings
Fig. 1 comprehensively produces the process method flow chart of Radix Puerariae total flavones, puerarin and pueraria starch for the present invention.
Embodiment
The invention will be further described below in conjunction with accompanying drawing.
As shown in Figure 1, the processing method that a kind of advantages of simplicity and high efficiency that present embodiment provides is comprehensively produced Radix Puerariae total flavones, puerarin and pueraria starch, its steps flow chart is as follows:
(1) the fresh root of kudzu vine is cleaned section, carry out drying, crushing screening is weighed.
(2) getting granularity is the 80 purpose roots of kudzu vine, pre-bubble 1 hour, its solid-to-liquid ratio is 1g: 25ml, and under normal pressure, adopting power is that the microwave of 800W is to wetting root of kudzu vine intermittent radiation 3 times, each radiation 40 seconds, adopting concentration is that 80% alcohol solvent is to wetting root of kudzu vine extraction, 80 ℃ of extraction temperature, 60 minutes extraction time, after cooling, the filtration, promptly obtain Radix Puerariae total flavones extraction liquid and filter residue.
(3) with Radix Puerariae total flavones extraction liquid elder generation evaporating solvent, be 95% ethanol sedimentation water-soluble impurity with concentration again, evaporating solvent promptly obtains spissated Radix Puerariae total flavones solution once more.
(4-1) to AB-8 resin columns in series absorption on the Radix Puerariae total flavones solution, its sample solution is that spissated Radix Puerariae total flavones strength of solution is 6.6931mg/ml, and the sample solution amount is 3 times of AB-8 resin columns in series volumes; Absorption back distilled water wash-out, 70% ethanol elution is collected elutriant, concentrates, and the vacuum decompression drying can obtain the Radix Puerariae total flavones product of content 91.0%.
(4-2) to AB-8 resin columns in series absorption on the Radix Puerariae total flavones solution, its sample solution is that spissated Radix Puerariae total flavones strength of solution is 6.6931mg/ml, and the sample solution amount is 3 times of AB-8 resin columns in series volumes; Absorption back distilled water wash-out, be washed to colourless after, 70% ethanol elution, collect elutriant 50ml-100ml (repetition test proves that in the above conditions, puerarin elutes in this scope), concentrate, the vacuum decompression drying can obtain purity and reach 98.0% puerarin products.
(5) gained filter residue in the step (2) is soaked, after twice of 180 mesh sieves filtration,, can be obtained the pueraria starch of content 92.21% after the precipitation drying the centrifugation of gained filtrate.
Claims (4)
1. comprehensive processing method of producing Radix Puerariae total flavones, puerarin and pueraria starch is characterized in that this method may further comprise the steps:
(1) the fresh root of kudzu vine is cleaned section, carry out drying, crushing screening is weighed;
(2) will carry out wettingly through the above-mentioned pretreated root of kudzu vine, and under normal pressure, adopt 80% ethanol microwave auxiliary extraction, cold filtration promptly obtains Radix Puerariae total flavones extraction liquid and filter residue;
(3) the Radix Puerariae total flavones extraction liquid is concentrated, promptly obtain spissated Radix Puerariae total flavones solution;
(4-1) to AB-8 resin columns in series absorption on the spissated Radix Puerariae total flavones solution, the distilled water wash-out, 70% ethanol elution is collected elutriant, concentrates, and the vacuum decompression drying promptly obtains the Radix Puerariae total flavones product;
(4-2) to AB-8 resin columns in series absorption on the spissated Radix Puerariae total flavones solution, the distilled water wash-out, be washed to colourless after, 70% ethanol elution is collected elutriant 50ml to 100ml, concentrates, the vacuum decompression drying promptly obtains puerarin;
(5) gained filter residue in the step (2) is soaked, after twice of 180 mesh sieves filtration,, promptly obtained pueraria starch after the precipitation drying the centrifugation of gained filtrate.
2. the processing method of comprehensive production Radix Puerariae total flavones according to claim 1, puerarin and pueraria starch, it is characterized in that: will carry out wetting through the above-mentioned pretreated root of kudzu vine described in the step (2), adopting 80% ethanol microwave auxiliary extraction operation specifically is that to get granularity earlier be the 80 purpose roots of kudzu vine, pre-bubble 1 hour, its solid-to-liquid ratio is 1g: 25ml, adopting power again is that the microwave of 800W is to wetting root of kudzu vine intermittent radiation 3 times, each radiation 40 seconds, adopting concentration is that 80% alcohol solvent is to wetting root of kudzu vine extraction, 80 ℃ of extraction temperature, 60 minutes extraction time.
3. the processing method of comprehensive production Radix Puerariae total flavones according to claim 1, puerarin and pueraria starch, it is characterized in that: the concentration operation described in the step (3) specifically is first evaporating solvent, use 95% ethanol sedimentation water-soluble impurity, evaporating solvent once more again.
4. the processing method of comprehensive production Radix Puerariae total flavones according to claim 1, puerarin and pueraria starch, it is characterized in that: spissated Radix Puerariae total flavones strength of solution is 6.6931mg/ml in the last AB-8 resin columns in series adsorption operations described in step (4-1) and the step (4-2), and the sample solution amount is 3 times of AB-8 resin columns in series volumes.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102973667A (en) * | 2012-10-15 | 2013-03-20 | 李正梅 | Preparation method and application of cold-fever tablet |
| CN102988465A (en) * | 2012-10-24 | 2013-03-27 | 赤壁元一生物科技有限公司 | Process for low-temperature extracting kudzu root flavanone and kudzu root starch from kudzu root |
| CN103254185A (en) * | 2013-05-25 | 2013-08-21 | 湖北省三鑫生物科技有限公司 | Novel process for producing puerarin by utilizing root of kudzu vine |
| CN103478533A (en) * | 2013-09-25 | 2014-01-01 | 湖北省宏源药业有限公司 | Puerarin-rich instant arrowroot and preparation method thereof |
| CN103554276A (en) * | 2013-10-15 | 2014-02-05 | 温天文 | Technological process for extracting starch, cellulose and general flavone in root of kudzu vine by use of organic membrane |
| CN104873569A (en) * | 2015-06-23 | 2015-09-02 | 四川大学 | Method for efficiently and synchronously extracting pueraria starch and flavone |
| CN105687307A (en) * | 2016-03-10 | 2016-06-22 | 合肥九研医药科技开发有限公司 | Composition containing kudzuvine roots and preparation method of composition |
| CN106519046A (en) * | 2016-11-05 | 2017-03-22 | 湖北省农业科学院中药材研究所 | Preparation method of flavone-enriched pueraria lobata starch |
| CN106731201A (en) * | 2016-12-12 | 2017-05-31 | 十堰市绿田生物科技有限公司 | Pueraria starch runner type settler |
| CN107935928A (en) * | 2017-12-06 | 2018-04-20 | 鲁东大学 | A kind of total diphenylpypazole and its preparation method and application |
| CN111264586A (en) * | 2020-03-04 | 2020-06-12 | 四川健腾生物技术有限公司 | Radix puerariae composite biscuit composition and preparation method thereof |
| CN111358828A (en) * | 2019-12-06 | 2020-07-03 | 陕西中医药大学 | Alcohol effect dispelling essential oil and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101550133A (en) * | 2009-05-13 | 2009-10-07 | 南阳市海达生物技术有限公司 | Method for extracting puerarin and usage of kudzu vine root for preparing medicament protecting liver |
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- 2010-03-29 CN CN2010101362181A patent/CN101805410B/en not_active Expired - Fee Related
Patent Citations (1)
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| CN101550133A (en) * | 2009-05-13 | 2009-10-07 | 南阳市海达生物技术有限公司 | Method for extracting puerarin and usage of kudzu vine root for preparing medicament protecting liver |
Non-Patent Citations (2)
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| 《安徽农业科学》 20070930 李岂凡等 从葛根中提取和纯化药用成分--总黄酮 8680-8681 权利要求1-4 第35卷, 第27期 2 * |
| 《食品工业科技》 19980215 陆宁，宛晓春 葛根总黄酮、淀粉的提取及应用 32-34 权利要求1-4 , 第1期 2 * |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102973667A (en) * | 2012-10-15 | 2013-03-20 | 李正梅 | Preparation method and application of cold-fever tablet |
| CN102988465A (en) * | 2012-10-24 | 2013-03-27 | 赤壁元一生物科技有限公司 | Process for low-temperature extracting kudzu root flavanone and kudzu root starch from kudzu root |
| CN103254185A (en) * | 2013-05-25 | 2013-08-21 | 湖北省三鑫生物科技有限公司 | Novel process for producing puerarin by utilizing root of kudzu vine |
| CN103478533A (en) * | 2013-09-25 | 2014-01-01 | 湖北省宏源药业有限公司 | Puerarin-rich instant arrowroot and preparation method thereof |
| CN103478533B (en) * | 2013-09-25 | 2014-10-22 | 湖北省宏源药业有限公司 | Puerarin-rich instant arrowroot and preparation method thereof |
| CN103554276A (en) * | 2013-10-15 | 2014-02-05 | 温天文 | Technological process for extracting starch, cellulose and general flavone in root of kudzu vine by use of organic membrane |
| CN104873569A (en) * | 2015-06-23 | 2015-09-02 | 四川大学 | Method for efficiently and synchronously extracting pueraria starch and flavone |
| CN105687307A (en) * | 2016-03-10 | 2016-06-22 | 合肥九研医药科技开发有限公司 | Composition containing kudzuvine roots and preparation method of composition |
| CN106519046A (en) * | 2016-11-05 | 2017-03-22 | 湖北省农业科学院中药材研究所 | Preparation method of flavone-enriched pueraria lobata starch |
| CN106519046B (en) * | 2016-11-05 | 2018-12-25 | 湖北省农业科学院中药材研究所 | Rich in flavones Radix Puerariae starch preparation method |
| CN106731201A (en) * | 2016-12-12 | 2017-05-31 | 十堰市绿田生物科技有限公司 | Pueraria starch runner type settler |
| CN107935928A (en) * | 2017-12-06 | 2018-04-20 | 鲁东大学 | A kind of total diphenylpypazole and its preparation method and application |
| CN107935928B (en) * | 2017-12-06 | 2021-05-25 | 鲁东大学 | Total diphenylpyrazole and preparation method and application thereof |
| CN111358828A (en) * | 2019-12-06 | 2020-07-03 | 陕西中医药大学 | Alcohol effect dispelling essential oil and preparation method and application thereof |
| CN111264586A (en) * | 2020-03-04 | 2020-06-12 | 四川健腾生物技术有限公司 | Radix puerariae composite biscuit composition and preparation method thereof |
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