CN102461898A - Technological process for extracting flavone from jujube fruits - Google Patents
Technological process for extracting flavone from jujube fruits Download PDFInfo
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- CN102461898A CN102461898A CN2010105416782A CN201010541678A CN102461898A CN 102461898 A CN102461898 A CN 102461898A CN 2010105416782 A CN2010105416782 A CN 2010105416782A CN 201010541678 A CN201010541678 A CN 201010541678A CN 102461898 A CN102461898 A CN 102461898A
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Abstract
The invention provides a technological process for extracting flavone from jujube fruits. The process is finished in the following steps of: (1) first, washing, drying and crushing the jujube fruits to obtain jujube fruit powder; (2) carrying out ethanol extraction, ultrasonic wall breaking technology and microwave-assisted extraction on the crushed jujube fruit powder; (3) carrying out biological enzyme hydrolysis on waste slag obtained in the step (2) to obtain soluble dietary fiber and decompressing and concentrating a filter liquor; (4) purifying the decompressed and concentrated filter liquor by using a macroporous adsorption resin to obtain a concentrated solution with pH value of 4; and (5) freezing and drying the concentrated solution to obtain jujube flavone. The technological process for extracting flavone from jujube fruits, provided by the invention, has the following advantages that: (1) short production time, low operation cost and small energy usage are obtained; (2) the purity of the extracted flavone can reach more than 45% and the yield of the flavone is no less than 80%; and (3) the jujube fruit waste slag after the flavone is extracted can be hydrolyzed by biological enzyme to obtain the dietary fiber with wide purposes so that the comprehensive utilization rate of the jujube fruit is increased.
Description
Technical field:
The invention belongs to a kind of food-processing method, particularly a kind of process of from the jujube fruit, extracting flavones.
Background technology:
Flavone compound is one a type low molecule natural plant composition, is the aldehydes matter that occurring in nature exists, and claims biological flavone or plant flavone again, and the platymiscium secondary metabolite extensively is present in each kind of plant and the macro fungi.Flavone compound has certain pharmaceutical property, as reduce MCO, increase coronary artery and cerebrovascular flow, reduce blood sugar, anti-oxidant, eliminate functions such as interior free yl, anti-ageing, enhancing body immunity.In recent years, people hanker after the exploitation of natural plant, so flavone compound has more and more caused gazing at of people with the pharmacological action of its wide spectrum.
At present a lot of to the method for distilling of natural flavone compounds, like solvent extraction method, supercritical fluid extraction, Microwave Extraction method, enzymatic isolation method, ultrasonic extraction, aqueous two-phase extraction partition method etc.The flavone compound that these methods are extracted remains a mixture, is the bullion that contains other impurity, needs further separation and purification, and method commonly used has column chromatography, recrystallization method, lead salt precipitation and high performance liquid chromatography etc.
Therefore, above-mentioned these extract chromocor compounds method process route length, complex process, the production time is long, production cost is high, and the purity of extracting and yield can be above 30%.
China is jujube tree plantation big country, produces more than 140 ten thousand tons of bright jujubes per year, accounts for 98% of the world.Jujube class processing technology is ripe, of a great variety, be rich in a large amount of flavones and cellulose in the jujube fruit, and the flavones purity after the method for extraction flavones is extracted from the jujube fruit now is not high, and low to the utilization rate of jujube fruit.
Summary of the invention:
The object of the invention just is to provide a kind of process of from the jujube fruit, extracting flavones; This method really is a raw material with jujube; Carry the flavones in taking out date pit through the resin column chromatographic technique, it is low that production technology is simple, one-tenth produces cost, and the flavones purity after extracting is more than 45%.
As above design, technical scheme of the present invention is: a kind of process of from the jujube fruit, extracting flavones is characterized in that: accomplish according to the following step:
(1) at first jujube is really cleaned up, descend drying at 40-50 ℃, be ground into 100 purpose jujubes fruit powder;
(2) fruit of the jujube after will pulverizing powder passes through alcohol extract, supersonic wave wall breaking technology and microwave assisted extraction successively;
(3) waste residue that step (2) is obtained goes out soluble dietary fiber through the biological enzymolysis process for producing, and filtrating reduce pressure concentrated;
(4) filtrating that will reduce pressure after concentrating is crossed post through macroporous absorbent resin through twice, and it is 4 concentrate that 50% ethanol elution obtains the pH value;
(5) above-mentioned concentrate is then obtained flavones content at the jujube flavones more than 45% through after the freeze drying.
The process conditions of above-mentioned alcohol extract are under 70 degrees centigrade, and 50% ethanol, solid-liquid ratio extract flavones 2 time, each 2 hour at 1: 15.
Above-mentioned supersonic wave wall breaking technology is auxiliary power 250W supersonic wave wall breaking 2 times, each 1 hour.
Above-mentioned microwave assisted extraction is to utilize the 500W microwave power to extract each 5 minutes 2 times.
The condition that above-mentioned biological enzymolysis is handled waste residue is: use cellulase to be hydrolyzed, the pH value is 4.5-5.5, and enzyme concentration is 0.2%, hydrolysis temperature 50-55 degree centigrade, and enzymolysis time 3 hours.
The present invention has following advantage and good effect:
1, the present invention is that collection microwave technology, ultrasonic disruption technology, Freeze Drying Technique, decompression concentration technique, resin adsorption technology be the process of one, makes that the production of extraction flavones has the advantages that the production time is short, operation cost is low, energy use is few from jujube nuclear.
2, the present invention is really to be raw material with jujube, and raw material sources are abundant, thereby further reduced cost.
3, the flavones purity that extracts of the present invention can reach more than 45%, and yield is not less than 80%.
4, the jujube fruit waste residue that is generated behind the extraction brass can pass through the biological enzymolysis process for producing and go out broad-spectrum dietary fiber, has improved the comprehensive utilization ratio of jujube fruit.
Description of drawings:
Fig. 1 is a process chart of the present invention.
The specific embodiment:
A kind of process of from the jujube fruit, extracting flavones, accomplish according to the following step:
(1) at first jujube is really cleaned up, the following drying of 50 degrees centigrade constant temperature also is ground into 100 purpose jujubes nuclear powder;
(2) fruit of the jujube after will pulverizing powder adopts under 70 degrees centigrade successively, 50% ethanol, solid-liquid ratio extracts flavones 2 time, each 2 hour at 1: 15; Auxiliary power 250W supersonic wave wall breaking 2 times extracted each 5 minutes 2 times with the 500W microwave power in each 1 hour;
(3) waste residue that step (2) is obtained goes out soluble dietary fiber through the biological enzymolysis process for producing, and filtrating reduce pressure concentrated; Wherein: the condition that biological enzymolysis is handled waste residue is: use cellulase to be hydrolyzed, the pH value is 4.5-5.5, and enzyme concentration is 0.2%, hydrolysis temperature 50-55 degree centigrade, and enzymolysis time 3 hours;
(4) filtrating that will reduce pressure after concentrating is crossed post through macroporous absorbent resin through twice, and it is 4 that 50% ethanol elution obtains the pH value, and sample liquid concentration is the concentrate of 0.8-1.2mg/mL;
(5) above-mentioned concentrate is then obtained flavones content at the jujube flavones more than 45% through after the freeze drying.
Claims (5)
1. process of from jujube fruit, extracting flavones is characterized in that: accomplish according to the following step:
(1) at first jujube is really cleaned up, dry under the temperature of 40-50, be ground into 100 purpose jujubes nuclear powder;
(2) fruit of the jujube after will pulverizing powder passes through alcohol extract, supersonic wave wall breaking technology and microwave assisted extraction successively;
(3) waste residue that step (2) is obtained goes out soluble dietary fiber through the biological enzymolysis process for producing, and filtrating reduce pressure concentrated;
(4) filtrating that will reduce pressure after concentrating is crossed post through macroporous absorbent resin through twice, and it is 4 concentrate that 50% ethanol elution obtains the pH value;
(5) above-mentioned concentrate is then obtained flavones content at the jujube flavones more than 45% through after the freeze drying.
2. a kind of process of from jujube nuclear, extracting flavones according to claim 1, it is characterized in that: the process conditions of above-mentioned alcohol extract are under 70 degrees centigrade, 50% ethanol, solid-liquid ratio extract flavones 2 time, each 2 hour at 1: 15.
3. a kind of process of from jujube nuclear, extracting flavones according to claim 1, it is characterized in that: above-mentioned supersonic wave wall breaking technology is auxiliary power 250W supersonic wave wall breaking 2 times, each 1 hour.
4. a kind of process of from jujube nuclear, extracting flavones according to claim 1 is characterized in that: above-mentioned microwave assisted extraction is to utilize the 500W microwave power to extract each 5 minutes 2 times.
5. a kind of process of from jujube nuclear, extracting flavones according to claim 1; It is characterized in that: the condition that above-mentioned biological enzymolysis is handled waste residue is: use cellulase to be hydrolyzed, the pH value is 4.5-5.5, and enzyme concentration is 0.2%; Hydrolysis temperature 50-55 degree centigrade, enzymolysis time 3 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2010105416782A CN102461898A (en) | 2010-11-12 | 2010-11-12 | Technological process for extracting flavone from jujube fruits |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010105416782A CN102461898A (en) | 2010-11-12 | 2010-11-12 | Technological process for extracting flavone from jujube fruits |
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| CN102461898A true CN102461898A (en) | 2012-05-23 |
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| CN2010105416782A Pending CN102461898A (en) | 2010-11-12 | 2010-11-12 | Technological process for extracting flavone from jujube fruits |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102816640A (en) * | 2012-09-05 | 2012-12-12 | 无锡华海香料有限公司 | Preparation method of jujube extract and application thereof |
| CN103284320A (en) * | 2013-06-09 | 2013-09-11 | 红云红河烟草(集团)有限责任公司 | Date extractive and application of date extractive in cigarette |
| CN104312921A (en) * | 2014-09-28 | 2015-01-28 | 广州英佩尔电子科技有限公司 | Comprehensive micro-molecule wall breaking method used for fresh food and drug materials |
| CN106418107A (en) * | 2016-09-20 | 2017-02-22 | 湖南农业大学 | Millet jujube flavone and polysaccharide compounding substance effervescent tablets and preparation method thereof |
| CN109123754A (en) * | 2018-09-20 | 2019-01-04 | 云南中烟工业有限责任公司 | A kind of high efficiency preparation method and purposes of jujube absolute oil |
| CN112915126A (en) * | 2021-03-04 | 2021-06-08 | 长治医学院 | Method for extracting red date flavonoid compound |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101288468A (en) * | 2008-05-23 | 2008-10-22 | 山西省农业科学院农产品综合利用研究所 | Method of sequential extraction of biological activity component from jujube |
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2010
- 2010-11-12 CN CN2010105416782A patent/CN102461898A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101288468A (en) * | 2008-05-23 | 2008-10-22 | 山西省农业科学院农产品综合利用研究所 | Method of sequential extraction of biological activity component from jujube |
Non-Patent Citations (2)
| Title |
|---|
| 姚文华等: "酶法制备枣膳食纤维与应用的研究", 《食品科学》, vol. 28, no. 1, 31 December 2007 (2007-12-31) * |
| 胡迎芬等: "大孔吸附树脂纯化冬枣总黄酮的研究", 《青岛大学学报（工程技术版）》, vol. 23, no. 2, 30 June 2008 (2008-06-30) * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102816640A (en) * | 2012-09-05 | 2012-12-12 | 无锡华海香料有限公司 | Preparation method of jujube extract and application thereof |
| CN102816640B (en) * | 2012-09-05 | 2013-08-07 | 无锡华海香料有限公司 | Preparation method of jujube extract and application thereof |
| CN103284320A (en) * | 2013-06-09 | 2013-09-11 | 红云红河烟草(集团)有限责任公司 | Date extractive and application of date extractive in cigarette |
| CN104312921A (en) * | 2014-09-28 | 2015-01-28 | 广州英佩尔电子科技有限公司 | Comprehensive micro-molecule wall breaking method used for fresh food and drug materials |
| CN106418107A (en) * | 2016-09-20 | 2017-02-22 | 湖南农业大学 | Millet jujube flavone and polysaccharide compounding substance effervescent tablets and preparation method thereof |
| CN109123754A (en) * | 2018-09-20 | 2019-01-04 | 云南中烟工业有限责任公司 | A kind of high efficiency preparation method and purposes of jujube absolute oil |
| CN109123754B (en) * | 2018-09-20 | 2021-02-26 | 云南中烟工业有限责任公司 | Efficient preparation method and application of red date absolute |
| CN112915126A (en) * | 2021-03-04 | 2021-06-08 | 长治医学院 | Method for extracting red date flavonoid compound |
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Application publication date: 20120523 |