CN103013648A - Method for preparing vegetable seed kernel oil and reclaiming protein through assistance of biological enzyme - Google Patents
Method for preparing vegetable seed kernel oil and reclaiming protein through assistance of biological enzyme Download PDFInfo
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Abstract
The invention relates to a method for preparing vegetable seed kernel oil and reclaiming protein through assistance of biological enzyme. The method comprises the following steps: 1) preparing and preprocessing vegetable seed kernels; 2) crushing and tempering the vegetable seed kernels; 3) freezing and unfreezing the vegetable seed kernels; 4) grinding the vegetable seed kernels into thick liquid and performing enzymolysis and digestion on the vegetable seed kernels, wherein the step can be realized in that hot water is added in unfrozen squeezed vegetable seed kernel powder for grinding the thick liquid in a colloid mill to obtain squeezed vegetable seed kernel thick liquid, and the mass of the hot water is 2-6 times of that of the vegetable seed kernel powder; the squeezed vegetable seed kernel thick liquid is passed in an ultrasonic reaction kettle which is provided with a mechanical stirring device; the pH value of the squeezed vegetable seed kernel thick liquid is adjusted within 8.0-10.0 by using NaOH solution; after alkaline protease is added, mechanical stirring and an ultrasonic generator are started to carry out the enzymolysis reaction; the total reaction time is not less than 1 hour; the mass of the alkaline protease is 0.5-1.5 percent of that of the squeezed vegetable seed kernel powder; and then the vegetable seed kernel thick liquid is obtained; and 5) separating oil and reclaiming the protein. The method has high oil yield.
Description
Technical field
The present invention relates to the auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme, belong to the applied technical field of biological enzyme technology in grease processing.
Background technology
In oil prodution industry, the auxiliary grease extractive technique of enzyme process undoubtedly is a kind of emerging technology.It is compared with traditional extracting method and has many good qualities, such as, the enzyme process auxiliary process does not have the consumption of solvent substantially, and the investment cost is lower, and energy requirement is also less.In addition, the auxiliary grease extraction process of enzyme process can be from oilseeds simultaneously refiltered oil and albumen, the oil quality of output is good, meets the requirement of national standard; This technique is extracted the oil that obtains not to be needed further to come unstuck, and some toxin and anti-nutrition component in the oilseeds also are removed in the course of processing.In a sense, some are such as saving cost, and the demands such as protection of the environment, safety in production and enhancing nutrition have triggered the innovation of grease extractive technique.Assist oil extracting process and successful Application in production by the development enzyme process, just can well satisfy these demands.
Within nearest five times more than ten years, extract oil technology with aqua method from oilseeds and some researchs, launch.Although this concept has potential magnetism, but the oil extraction technique with traditional organic solvent based on normal hexane, ether and No. 6 solvents etc. is compared, the grease yield of aqua method oil extracting process is lower, the residual oil content that in the filter residue (sometimes is in protein isolate) is high, and this has hindered the commercial applications of aqua method grease extractive technique.Some studies show that, the enzyme that has hydrolytic action by use can solve the low problem of aqua method grease extraction yield.Thereby these enzymes can help oil to discharge increase grease yield in oilseeds.In addition, environmental problem, particularly growing care to the volatile organic compounds that produced by the machine solvent are so that the auxiliary oil extracting process of enzyme process becomes the focus that people pay close attention to.
Start late to the research of the auxiliary liquefaction of enzyme process in domestic aspect, 2004, the Liu Zhiqiang philosophy adopted prozyme (proteolytic enzyme+cellulase+polygalacturonase), proteolytic enzyme, cellulase, polygalacturonase and hemicellulase that rapeseed decorticate is carried out aqueous enzymatic method and gets oil.Result of study shows, different enzymes affect different on oil and albumen yield, and prozyme is the highest, and the proteolytic enzyme effect is more obvious, and cellulase takes second place, and the three all is higher than without the enzyme aqua and extracts.Domestic patent aspect, mainly disclose at present the oil plants such as tea seed (ZL200910039039.3), peanut (ZL 200810021343.0), maize germ (ZL200410014630.0), sunflower seeds (CN1952094A) and sesame (CN101224022A) are adopted enzyme process assisted Extraction oil and albumen, the patented technology that yet there are no vegetable seed or vegetable seed benevolence (desquamated rapeseed) enzyme process assisted Extraction oil and albumen is open.From above-mentioned disclosed technology, ubiquity grease yield is lower, water consumption is large, add that the enzyme class is more, enzymolysis time is long, need constantly to regulate the pH value with problems such as the requirement that adapts to different process and energy consumption are higher.
Summary of the invention
Problem to be solved by this invention is to provide a kind of biological enzyme the auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen for the deficiency of above-mentioned prior art existence, and the method grease yield is high.
The present invention is that the technical scheme that adopts of the problem of the above-mentioned proposition of solution is as follows:
The auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme is characterized in that it comprises the steps:
1) producing and pre-treatment of vegetable seed benevolence: obtain vegetable seed benevolence with shelling in the vegetable seed raw material input peeler with separation of hull and kernel, skin-containing rate is controlled between 2～8wt% in its vegetable seed benevolence; Then vegetable seed benevolence is heated to 90～110 ℃ and keep 5～15min with open steam;
2) fragmentation of vegetable seed benevolence and modified: vegetable seed benevolence is put in the double spiral squeezer, carried out at normal temperatures fragmentation and pre-pressing, obtain vegetable seed benevolence normal temperature squeezing crude oil and vegetable seed benevolence squeeze cake; The vegetable seed benevolence squeeze cake that obtains is pulverized, made it pass through 10～60 mesh standard sieves, obtain squeezing vegetable seed benevolence powder; In squeezing vegetable seed benevolence powder, add entry, make its average moisture adjust to 20～50wt%;
3) vegetable seed benevolence freezing with thaw: be that to put into temperature be that-4～-18 ℃ freezing plant carries out taking out behind freezing 2～6h and thaws to 20～50wt% squeezing vegetable seed benevolence powder with moisture;
4) vegetable seed benevolence defibrination and Enzymolysis: the hot water (hot water temperature is 45～85 ℃) that adds 2～6 times of its quality in the squeezing vegetable seed benevolence powder that thaws carries out defibrination in colloidal mill, obtains squeezing vegetable seed benevolence slurry;
Squeezing vegetable seed benevolence slurries are sent in the ultrasonic response still with mechanical stirring device, (concentration is that 0.5～1mol/L) the pH value that will squeeze vegetable seed benevolence slurries adjusts to 8.0～10.0 with NaOH solution, and adding squeezing vegetable seed benevolence powder (is by 10～60 mesh standard sieves, the squeezing vegetable seed benevolence powder that obtains) behind the Sumizyme MP of quality 0.5～1.5%, open mechanical stirring and ultrasonic generator and carry out enzyme digestion reaction, total reaction time is not less than 1h, obtains vegetable seed benevolence slurries;
5) oil separating and albumen reclaim: after enzyme digestion reaction is finished, vegetable seed benevolence slurries are sent to carry out oily water separation and solid-liquid separation in the whizzer, obtain vegetable seed benevolence edible vegetable oil (being vegetable seed benevolence oil), emulsion layer, protein solution and slag; Protein solution obtains vegetable seed aqueous soluble protein or polypeptide (being the albumen of required recovery) after the concentrated spray drying;
Emulsion layer skimmed with slag blanket (being slag) merge, after again adding water and carry out enzymolysis by the method in the step 4), again centrifugation, so repeat 1～5 time, until residual oil content [being the ratio of oleaginousness and the oleaginousness in the raw material vegetable seed benevolence powder (being by 10～60 mesh standard sieves the squeezing vegetable seed benevolence powder that obtains) of slag] is not more than 5wt% relatively; Merge separating the vegetable seed benevolence edible vegetable oil that obtains behind each enzymolysis, obtain vegetable seed benevolence oil after the vacuum-drying; To obtain the desquamated rapeseed dregs of rice after the slag vacuum-drying that obtain at last;
Step 2) the vegetable seed benevolence normal temperature squeezing crude oil that obtains in is filtered through essence, obtains vegetable seed benevolence normal temperature pressed oil (being vegetable seed benevolence oil) after the deodorizing.
Press such scheme, step 1) described with vegetable seed benevolence with after the open steam heating, make its moisture adjust to 6～18wt%.
Press such scheme, step 2) the described employing double spiral squeezer Residual oil content that squeezes at normal temperatures the vegetable seed benevolence squeeze cake that obtains is 10～26wt%.
Press such scheme, step 4) described in the squeezing vegetable seed benevolence powder that thaws added hot water temperature be 45～85 ℃.
Press such scheme, step 4) the enzyme digestion reaction temperature of described squeezing vegetable seed benevolence slurry in the ultrasonic response still is 45～75 ℃, mechanical stirring speed is 120～300r/min, ultrasonic frequency is 20～98KHz, each Ultrasonic Radiation pitch time is 5～60min, and each Ultrasonic Radiation time length is 10～30min.
Press such scheme, described emulsion layer is skimmed and slag blanket merges, and then after adding water and carrying out enzymolysis, again centrifugation, the multiplicity of whole process is 1～5 time.
The present invention is according to the structure compositing characteristic of vegetable seed benevolence, utilize first the broken vegetable seed benevolence of physics mode, adopt again the enzyme preparation degrades vegetable seed benevolence that filters out, and unite freezing and the ultrasonic technology co-treatment, thereby promote the release of grease in the vegetable seed benevolence and increase protein hydrolysis degree, further improve the grease yield.
Beneficial effect of the present invention is:
1) adopts desquamated rapeseed as raw material, and before the enzymolysis fragmentation, adopt wet heat treatment, make the abundant passivation of endogenous enzyme energy and inactivation in the vegetable seed, avoided the generation of some antinutritional factor, improved the quality of oil and albumen.
2) not that squeezing vegetable seed benevolence fuel-displaced is purpose as far as possible, but take suitable broken vegetable seed benevolence as purpose, adopt double spiral squeezer that vegetable seed benevolence is processed, can suitably turn down the gun pressure power of pressing, increase the output of unit time, energy consumption and mechanical wear are reduced.Compare simultaneously all right high-quality grease of recovery part with the breaking method (such as toothed roll crusher) that adopts other, reduce the pressure that the back enzymolysis is put forward oily operation.
3) thus adopting slow freezing to make to form larger ice crystal in the broken vegetable seed benevolence cell is fully destroyed vegetable seed benevolence cellularstructure, help the enzymolysis of proteolytic enzyme, grease can fully be discharged; Adopt ultrasonic wave to come assistance enzymolysis, can strengthen Pignus pignoris efficient, reduce the emulsification degree of oil in water, be conducive to oily water separation and reduce enzyme processing time, improve the oil recovery rate.
4) behind the enzymolysis, after the slurries centrifugation, emulsion layer and slag blanket are added water more again carry out enzymolysis, reduced the inhibition of product to enzyme digestion reaction, make enzyme digestion reaction more abundant, grease is obtained through refining more thorough.
Embodiment
In order to understand better the present invention, further illustrate content of the present invention below in conjunction with embodiment, it is included within the scope of protection of the invention, but does not limit the present invention.
Embodiment 1
The auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme, it comprises the steps:
1) producing and pre-treatment of vegetable seed benevolence: the vegetable seed after vibratory screening apparatus, stone remover, magnetic separator, selection by winnowing is dropped into peeler shell, regulate the peeler parameter, make shelling obtain by separation of hull and kernel that skin-containing rate is controlled at 6wt% in the vegetable seed benevolence.Then vegetable seed benevolence is transported in the modified storehouse and heats (vapor temperature is 105 ℃) and keep 10min with open steam, make vegetable seed benevolence moisture adjust to 18wt%.
2) fragmentation of vegetable seed benevolence and modified: the vegetable seed benevolence after moisture adjusted is transported to the double spiral squeezer from modified storehouse, carries out at normal temperatures fragmentation and pre-pressing, obtains vegetable seed benevolence normal temperature squeezing crude oil and vegetable seed benevolence squeeze cake (Residual oil is 19wt%).Vegetable seed benevolence normal temperature squeezing crude oil is transported in the fine filting machine and filters, and sends into then that vacuum-drying obtains vegetable seed benevolence pressed oil (being vegetable seed benevolence oil) in the vacuum deodorizing tower; Entering vibratory screening apparatus after vegetable seed benevolence squeeze cake is sent into and pulverized in the pulverizer sieves (cross 30 mesh standard sieves) and obtains squeezing vegetable seed benevolence powder.
3) will squeeze vegetable seed benevolence powder and be transported in the cool chamber and to add after suitable quantity of water makes its average moisture adjust to 40wt%, regulate after the cool chamber temperature carries out freezing 6h to-12 ℃, and the cool chamber temperature be adjusted to room temperature (30 ℃) thaw.
4) thaw complete after, carry out defibrination in the colloidal mill with adding 60 ℃ of left and right sides hot water of 5 times of its quality and be transported in the squeezing vegetable seed benevolence powder, obtain squeezing vegetable seed benevolence slurry.
Squeezing vegetable seed benevolence slurries are sent in the ultrasonic response still with mechanical stirring device, to squeeze the pH value of vegetable seed benevolence slurries with NaOH solution (concentration is 1mol/L) and adjust to 9.5, and adding squeezing vegetable seed benevolence powder (is by 30 mesh standard sieves, the squeezing vegetable seed benevolence powder that obtains) Sumizyme MP (Novozymes Company of quality 1.5%, Alcalase 2.4L), (ultrasonic frequency is 40KHz to open mechanical stirring (stirring velocity is 200r/min) and ultrasonic generator, each Ultrasonic Radiation pitch time is 10min, each Ultrasonic Radiation time length is 15min) carry out enzyme digestion reaction, hydrolysis temperature is controlled at 60 ℃, and total reaction time is 1.5h.
5) after enzymolysis is finished, vegetable seed benevolence slurries are sent to carry out oily water separation and solid-liquid separation in the disk centrifugal separator, obtain vegetable seed benevolence edible vegetable oil, emulsion layer, protein solution and slag; Protein solution be sent to concentrate in the vacuum decker after, be transported to again and carry out spraying drying in the spray-dryer and obtain vegetable seed aqueous soluble protein or polypeptide;
The emulsion layer that separates skimmed be transported to carrying out enzymolysis, centrifugation by same operational condition and step in the ultrasonic response still of mechanical stirring device after again adding water after merging with slag blanket, repeat operation 2 times.Merge and send into that vacuum-drying obtains vegetable seed benevolence oil in the vacuum deodorizing tower separating the vegetable seed benevolence edible vegetable oil obtain behind each enzymolysis; Obtain the desquamated rapeseed dregs of rice (residual oil content is 5wt% relatively) after the slag that obtains at last sent in the vacuum drying oven dehydration.[grease that obtains (vegetable seed benevolence pressed oil, vegetable seed benevolence oil) quality and raw material vegetable seed benevolence powder (are by 30 mesh standard sieves the grease yield, the squeezing vegetable seed benevolence powder that obtains) ratio of grease total mass in] be 90%, the oil that obtains does not need further refining just can reach national 3 grade standards, and the gained rape seed protein can replace soybean protein to use.
Embodiment 2
The auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme, it comprises the steps:
1) producing and pre-treatment of vegetable seed benevolence: the vegetable seed after vibratory screening apparatus, stone remover, magnetic separator, selection by winnowing is dropped into peeler shell, regulate the peeler parameter, make shelling obtain by separation of hull and kernel that skin-containing rate is controlled at about 5wt% in the vegetable seed benevolence.Then vegetable seed benevolence is transported in the modified storehouse and heats (vapor temperature is 108 ℃) and keep 8min with open steam, vegetable seed benevolence moisture is adjusted to about 16wt%.
2) fragmentation of vegetable seed benevolence and modified: the vegetable seed benevolence after moisture adjusted is transported to the double spiral squeezer from modified storehouse, carries out at normal temperatures fragmentation and pre-pressing, obtains vegetable seed benevolence normal temperature squeezing crude oil and vegetable seed benevolence squeeze cake (Residual oil is 18wt%).Vegetable seed benevolence normal temperature squeezing crude oil is transported in the fine filting machine and filters, and sends into then that vacuum-drying obtains vegetable seed benevolence pressed oil in the vacuum deodorizing tower; Entering vibratory screening apparatus after vegetable seed benevolence squeeze cake is sent into and pulverized in the pulverizer sieves (cross 20 mesh standard sieves) and obtains squeezing vegetable seed benevolence powder.
3) will squeeze vegetable seed benevolence powder and be transported in the cool chamber and to add after suitable quantity of water makes its average moisture adjust to 45wt%, regulate after the cool chamber temperature carries out freezing 5h to-15 ℃, and the cool chamber temperature be adjusted to room temperature (about 30 ℃) thaw.
4) thaw complete after, carry out defibrination in the colloidal mill with adding 70 ℃ of left and right sides hot water of 4.5 times of its quality and be transported in the squeezing vegetable seed benevolence powder, obtain squeezing vegetable seed benevolence slurry.
Squeezing vegetable seed benevolence slurries are sent in the ultrasonic response still with mechanical stirring device, to squeeze the pH value of vegetable seed benevolence slurries with NaOH solution (concentration is 0.8mol/L) and adjust to 9.0, and the Sumizyme MP of adding squeezing vegetable seed benevolence opaque amount 1.2%, (ultrasonic frequency is 45KHz to open mechanical stirring (stirring velocity is 260r/min) and ultrasonic generator, each Ultrasonic Radiation pitch time is 20min, each Ultrasonic Radiation time length is 10min) carry out enzyme digestion reaction, hydrolysis temperature is controlled at 70 ℃, and total reaction time is 2.0h.
5) after enzymolysis is finished, vegetable seed benevolence slurries are sent to carry out oily water separation and solid-liquid separation in the disk centrifugal separator, obtain vegetable seed benevolence edible vegetable oil, emulsion layer, protein solution and slag; Protein solution be sent to concentrate in the vacuum decker after, be transported to again and carry out spraying drying in the spray-dryer and obtain vegetable seed aqueous soluble protein or polypeptide; Skim and be transported to carrying out enzymolysis, centrifugation by same operational condition and step in the ultrasonic response still of mechanical stirring device after again adding water after merging with slag blanket separating emulsion layer, repeat operation 3 times.Merge and send into that vacuum-drying obtains vegetable seed benevolence oil in the vacuum deodorizing tower separating the vegetable seed benevolence edible vegetable oil obtain behind each enzymolysis; Obtain the desquamated rapeseed dregs of rice (residual oil content is 4wt% relatively) after the slag that obtains at last sent in the vacuum drying oven dehydration.Grease yield (ratio of grease total mass in the oil quality that obtains and the raw material vegetable seed benevolence powder) is 92%, and the oil that obtains does not need further refining just can reach national 3 grade standards, and the gained rape seed protein can replace soybean protein to use.
Embodiment 3
The auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme, it comprises the steps:
1) producing and pre-treatment of vegetable seed benevolence: the vegetable seed after vibratory screening apparatus, stone remover, magnetic separator, selection by winnowing is dropped into peeler shell, regulate the peeler parameter, make shelling obtain by separation of hull and kernel that skin-containing rate is controlled at about 4wt% in the vegetable seed benevolence.Then vegetable seed benevolence is transported in the modified storehouse and heats (vapor temperature is 108 ℃) and keep 9min with open steam, vegetable seed benevolence moisture is adjusted to about 17wt%.
2) fragmentation of vegetable seed benevolence and modified: the vegetable seed benevolence after moisture adjusted is transported to the double spiral squeezer from modified storehouse, carries out at normal temperatures fragmentation and pre-pressing, obtains vegetable seed benevolence normal temperature squeezing crude oil and vegetable seed benevolence squeeze cake (Residual oil is 17wt%).Vegetable seed benevolence normal temperature squeezing crude oil is transported in the fine filting machine and filters, and sends into then that vacuum-drying obtains vegetable seed benevolence pressed oil in the vacuum deodorizing tower; Entering vibratory screening apparatus after vegetable seed benevolence squeeze cake is sent into and pulverized in the pulverizer sieves (cross 40 mesh standard sieves) and obtains squeezing vegetable seed benevolence powder.
3) will squeeze vegetable seed benevolence powder and be transported in the cool chamber and to add after suitable quantity of water makes its average moisture adjust to 48%, regulate after the cool chamber temperature carries out freezing 4h to-17 ℃, and the cool chamber temperature be adjusted to room temperature (30 ℃) thaw.
4) thaw complete after, carry out defibrination in the colloidal mill with adding 65 ℃ of left and right sides hot water of 4 times of its quality and be transported in the squeezing vegetable seed benevolence powder, obtain squeezing vegetable seed benevolence slurry.
Squeezing vegetable seed benevolence slurries are sent in the ultrasonic response still with mechanical stirring device, to squeeze the pH value of vegetable seed benevolence slurries with NaOH solution (concentration is 0.6mol/L) and adjust to 8.5, and the Sumizyme MP of adding squeezing vegetable seed benevolence opaque amount 1.0%, (ultrasonic frequency is 25KHz to open mechanical stirring (stirring velocity is 300r/min) and ultrasonic generator, each Ultrasonic Radiation pitch time is 18min, each Ultrasonic Radiation time length is 12min) carry out enzyme digestion reaction, hydrolysis temperature is controlled at 65 ℃, and total reaction time is 3.0h.
5) after enzymolysis is finished, vegetable seed benevolence slurries are sent to carry out oily water separation and solid-liquid separation in the disk centrifugal separator, obtain vegetable seed benevolence edible vegetable oil, emulsion layer, protein solution and slag; Protein solution be sent to concentrate in the vacuum decker after, be transported to again and carry out spraying drying in the spray-dryer and obtain vegetable seed aqueous soluble protein or polypeptide; Skim and be transported to carrying out enzymolysis, centrifugation by same operational condition and step in the ultrasonic response still of mechanical stirring device after again adding water after merging with slag blanket separating emulsion layer, repeat operation 1 time.Merge and send into that vacuum-drying obtains vegetable seed benevolence oil in the vacuum deodorizing tower separating the vegetable seed benevolence edible vegetable oil obtain behind each enzymolysis; Obtain the desquamated rapeseed dregs of rice (residual oil content is 4wt% relatively) after the slag that obtains at last sent in the vacuum drying oven dehydration.Grease yield (ratio of grease total mass in the oil quality that obtains and the raw material vegetable seed benevolence powder) is 91%, and the oil that obtains does not need further refining just can reach national 3 grade standards, and the gained rape seed protein can replace soybean protein to use.
Embodiment 4
The auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme, it comprises the steps:
1) producing and pre-treatment of vegetable seed benevolence: the vegetable seed after vibratory screening apparatus, stone remover, magnetic separator, selection by winnowing is dropped into peeler shell, regulate the peeler parameter, make shelling obtain by separation of hull and kernel that skin-containing rate is controlled at 2wt% in the vegetable seed benevolence.Then vegetable seed benevolence is transported in the modified storehouse and heats (vapor temperature is 110 ℃) and keep 5min with open steam, make vegetable seed benevolence moisture adjust to 6wt%.
2) fragmentation of vegetable seed benevolence and modified: the vegetable seed benevolence after moisture adjusted is transported to the double spiral squeezer from modified storehouse, carries out at normal temperatures fragmentation and pre-pressing, obtains vegetable seed benevolence normal temperature squeezing crude oil and vegetable seed benevolence squeeze cake (Residual oil is 10wt%).Vegetable seed benevolence normal temperature squeezing crude oil is transported in the fine filting machine and filters, and sends into then that vacuum-drying obtains vegetable seed benevolence pressed oil in the vacuum deodorizing tower; Entering vibratory screening apparatus after vegetable seed benevolence squeeze cake is sent into and pulverized in the pulverizer sieves (cross 10 mesh standard sieves) and obtains squeezing vegetable seed benevolence powder.
3) will squeeze vegetable seed benevolence powder and be transported in the cool chamber and to add after suitable quantity of water makes its average moisture adjust to 20wt%, regulate after the cool chamber temperature carries out freezing 2h to-4 ℃, and the cool chamber temperature be adjusted to room temperature (25 ℃) thaw.
4) thaw complete after, carry out defibrination in the colloidal mill with adding 45 ℃ of hot water of 2 times of its quality and be transported in the squeezing vegetable seed benevolence powder, obtain squeezing vegetable seed benevolence slurry.
Squeezing vegetable seed benevolence slurries are sent in the ultrasonic response still with mechanical stirring device, to squeeze the pH value of vegetable seed benevolence slurries with NaOH solution (concentration is 0.5mol/L) and adjust to 8.0, and the Sumizyme MP (the Properase 1600L of Genencor company) of adding squeezing vegetable seed benevolence opaque amount 0.5%, (ultrasonic frequency is 20KHz to open mechanical stirring (stirring velocity is 120r/min) and ultrasonic generator, each Ultrasonic Radiation pitch time is 5min, each Ultrasonic Radiation time length is 10min) carry out enzyme digestion reaction, hydrolysis temperature is controlled at 45 ℃, and total reaction time is 1.0h.
5) after enzymolysis is finished, vegetable seed benevolence slurries are sent to carry out oily water separation and solid-liquid separation in the disk centrifugal separator, obtain vegetable seed benevolence edible vegetable oil, emulsion layer, protein solution and slag; Protein solution be sent to concentrate in the vacuum decker after, be transported to again and carry out spraying drying in the spray-dryer and obtain vegetable seed aqueous soluble protein or polypeptide;
The emulsion layer that separates skimmed be transported to carrying out enzymolysis, centrifugation by same operational condition and step in the ultrasonic response still of mechanical stirring device after again adding water after merging with slag blanket, repeat operation 1 time.Merge and send into that vacuum-drying obtains vegetable seed benevolence oil in the vacuum deodorizing tower separating the vegetable seed benevolence edible vegetable oil obtain behind each enzymolysis; Obtain the desquamated rapeseed dregs of rice (residual oil content is 5wt% relatively) after the slag that obtains at last sent in the vacuum drying oven dehydration.Grease yield (ratio of grease total mass in the oil quality that obtains and the raw material vegetable seed benevolence powder) is 90%, and the oil that obtains does not need further refining just can reach national 3 grade standards, and the gained rape seed protein can replace soybean protein to use.
Embodiment 5
The auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme, it comprises the steps:
1) producing and pre-treatment of vegetable seed benevolence: the vegetable seed after vibratory screening apparatus, stone remover, magnetic separator, selection by winnowing is dropped into peeler shell, regulate the peeler parameter, make shelling obtain by separation of hull and kernel that skin-containing rate is controlled at 8wt% in the vegetable seed benevolence.Then vegetable seed benevolence is transported in the modified storehouse and heats (vapor temperature is 103 ℃) and keep 15min with open steam, make vegetable seed benevolence moisture adjust to 18wt%.
2) fragmentation of vegetable seed benevolence and modified: the vegetable seed benevolence after moisture adjusted is transported to the double spiral squeezer from modified storehouse, carries out at normal temperatures fragmentation and pre-pressing, obtains vegetable seed benevolence normal temperature squeezing crude oil and vegetable seed benevolence squeeze cake (Residual oil is 26wt%).Vegetable seed benevolence normal temperature squeezing crude oil is transported in the fine filting machine and filters, and sends into then that vacuum-drying obtains vegetable seed benevolence pressed oil in the vacuum deodorizing tower; Entering vibratory screening apparatus after vegetable seed benevolence squeeze cake is sent into and pulverized in the pulverizer sieves (cross 60 mesh standard sieves) and obtains squeezing vegetable seed benevolence powder.
3) will squeeze vegetable seed benevolence powder and be transported in the cool chamber and to add after suitable quantity of water makes its average moisture adjust to 50wt%, regulate after the cool chamber temperature carries out freezing 6h to-18 ℃, and the cool chamber temperature be adjusted to room temperature (20 ℃) thaw.
4) thaw complete after, carry out defibrination in the colloidal mill with adding 85 ℃ of hot water of 6 times of its quality and be transported in the squeezing vegetable seed benevolence powder, obtain squeezing vegetable seed benevolence slurry.
Squeezing vegetable seed benevolence slurries are sent in the ultrasonic response still with mechanical stirring device, to squeeze the pH value of vegetable seed benevolence slurries with NaOH solution (concentration is 1mol/L) and adjust to 10.0, and the Sumizyme MP (Novozymes Company of adding squeezing vegetable seed benevolence opaque amount 1.5%, Alcalase 2.4L), (ultrasonic frequency is 98KHz to open mechanical stirring (stirring velocity is 300r/min) and ultrasonic generator, each Ultrasonic Radiation pitch time is 60min, each Ultrasonic Radiation time length is 30min) carry out enzyme digestion reaction, hydrolysis temperature is controlled at 75 ℃, and total reaction time is 1.5h.
5) after enzymolysis is finished, vegetable seed benevolence slurries are sent to carry out oily water separation and solid-liquid separation in the disk centrifugal separator, obtain vegetable seed benevolence edible vegetable oil, emulsion layer, protein solution and slag; Protein solution be sent to concentrate in the vacuum decker after, be transported to again and carry out spraying drying in the spray-dryer and obtain vegetable seed aqueous soluble protein or polypeptide;
The emulsion layer that separates skimmed be transported to carrying out enzymolysis, centrifugation by same operational condition and step in the ultrasonic response still of mechanical stirring device after again adding water after merging with slag blanket, repeat operation 5 times.Merge and send into that vacuum-drying obtains vegetable seed benevolence oil in the vacuum deodorizing tower separating the vegetable seed benevolence edible vegetable oil obtain behind each enzymolysis; Obtain the desquamated rapeseed dregs of rice (residual oil content is 3wt% relatively) after the slag that obtains at last sent in the vacuum drying oven dehydration.Grease yield (ratio of grease total mass in the oil quality that obtains and the raw material vegetable seed benevolence powder) is 92%, and the oil that obtains does not need further refining just can reach national 3 grade standards, and the gained rape seed protein can replace soybean protein to use.
Claims (7)
1. the auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme is characterized in that it comprises the steps:
1) producing and pre-treatment of vegetable seed benevolence: obtain vegetable seed benevolence with shelling in the vegetable seed raw material input peeler with separation of hull and kernel, skin-containing rate is controlled between 2～8wt% in its vegetable seed benevolence; Then with vegetable seed benevolence with open steam heating and keep 5～15min;
2) fragmentation of vegetable seed benevolence and modified: vegetable seed benevolence is put in the double spiral squeezer, carried out at normal temperatures fragmentation and pre-pressing, obtain vegetable seed benevolence normal temperature squeezing crude oil and vegetable seed benevolence squeeze cake; The vegetable seed benevolence squeeze cake that obtains is pulverized, made it pass through 10～60 mesh standard sieves, obtain squeezing vegetable seed benevolence powder; In squeezing vegetable seed benevolence powder, add entry, make its average moisture adjust to 20～50wt%;
3) vegetable seed benevolence freezing with thaw: be that to put into temperature be that-4～-18 ℃ freezing plant carries out taking out behind freezing 2～6h and thaws to 20～50wt% squeezing vegetable seed benevolence powder with moisture;
4) vegetable seed benevolence defibrination and Enzymolysis: the hot water that adds 2～6 times of its quality in the squeezing vegetable seed benevolence powder that thaws carries out defibrination in colloidal mill, obtains squeezing vegetable seed benevolence slurry; Squeezing vegetable seed benevolence slurries are sent in the ultrasonic response still with mechanical stirring device, to squeeze the pH value of vegetable seed benevolence slurries with NaOH solution and adjust to 8.0～10.0, and after adding the Sumizyme MP of squeezing vegetable seed benevolence opaque amount 0.5～1.5%, open mechanical stirring and ultrasonic generator and carry out enzyme digestion reaction, total reaction time is not less than 1h, obtains vegetable seed benevolence slurries;
5) oil separating and albumen reclaim: after enzyme digestion reaction is finished, vegetable seed benevolence slurries are sent to carry out oily water separation and solid-liquid separation in the whizzer, obtain vegetable seed benevolence edible vegetable oil, emulsion layer, protein solution and slag; Protein solution obtains vegetable seed aqueous soluble protein or polypeptide after the concentrated spray drying;
Emulsion layer is skimmed and slag blanket merges, after again adding water and carry out enzymolysis by the method in the step 4), again centrifugation so repeats 1～5 time, until residual oil content is not more than 5wt% relatively; Merge separating the vegetable seed benevolence edible vegetable oil that obtains behind each enzymolysis, obtain vegetable seed benevolence oil after the vacuum-drying; To obtain the desquamated rapeseed dregs of rice after the slag vacuum-drying that obtain at last;
Step 2) the vegetable seed benevolence normal temperature squeezing crude oil that obtains in is filtered through essence, obtains vegetable seed benevolence normal temperature pressed oil after the deodorizing.
2. the auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme according to claim 1 is characterized in that step 1) described with vegetable seed benevolence with the open steam heating after, make its moisture adjust to 6～18wt%.
3. the auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme according to claim 1 is characterized in that step 2) the described employing double spiral squeezer Residual oil content that squeezes at normal temperatures the vegetable seed benevolence squeeze cake that obtains is 10～26wt%.
4. the auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme according to claim 1 is characterized in that step 4) described in the squeezing vegetable seed benevolence powder that thaws added hot water temperature be 45～85 ℃.
5. biological enzyme according to claim 1 is assisted the method for producing vegetable seed benevolence oil and reclaiming albumen, it is characterized in that, step 4) the enzyme digestion reaction temperature of described squeezing vegetable seed benevolence slurry in the ultrasonic response still is 45～75 ℃, mechanical stirring speed is 120～300r/min, ultrasonic frequency is 20～98KHz, each Ultrasonic Radiation pitch time is 5～60min, and each Ultrasonic Radiation time length is 10～30min.
6. the auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme according to claim 1 is characterized in that step 4) concentration of described NaOH solution is 0.5～1mol/L.
7. the auxiliary method of producing vegetable seed benevolence oil and reclaiming albumen of biological enzyme according to claim 1 is characterized in that step 1) temperature of described open steam heating is 90～110 ℃.
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| CN105713718A (en) * | 2016-01-25 | 2016-06-29 | 蚌埠市江淮粮油有限公司 | Method using millet bran to synchronously extract millet bran oil and millet bran protein |
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