CN101280327B - Synchronous aqueous enzymatic ultrasonic extraction for walnut oil and walnut protein peptide - Google Patents
Synchronous aqueous enzymatic ultrasonic extraction for walnut oil and walnut protein peptide Download PDFInfo
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- CN101280327B CN101280327B CN2008100584394A CN200810058439A CN101280327B CN 101280327 B CN101280327 B CN 101280327B CN 2008100584394 A CN2008100584394 A CN 2008100584394A CN 200810058439 A CN200810058439 A CN 200810058439A CN 101280327 B CN101280327 B CN 101280327B
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Abstract
The invention relates to an enzyme method ultrasonic extraction method of walnut oil and walnut protein, which belongs to the food and the functional food field. Water is added into walnut kernel or walnut powder to be grinded into paste, protease or compound protease are added into to be performed hydrolization, and simultaneously ultrasonic processing is performed, then the walnut kernel or walnut powder is agitated to perform enzymatic extraction, and then centrifugal separation oil phase, protein peptide oil water phase and residual solid phase are performed; walnut oil is acquired by refining the obtained oil phase, which meets the requirements of green foods; the protein peptide aqueous solution can be directly used to produce degreased walnut protein nutrient milk, or to prepare walnut antioxidation peptide after performed nanofiltration, which is used in health products, food additives, cosmetics or daily chemical articles, or to produce nutrient condensed milk after being performed low temperature concentrating, or to produce walnut protein nutrition powder after performed spray drying; the solid phase residue is prepared into diet fiber food after being dried and grinded into powder; walnut nutrient protein peptide products can be obtained through performing vacuum concentration and spray drying to walnut protein peptide extracting solution containing nutrient content.
Description
Technical field
The present invention relates to the synchronous aqueous enzymatic ultrasonic extraction of a kind of walnut oil and walnut protein peptide, belong to food or health food technology field.
Background technology
Walnut (Juglans regia L.) another name English walnut, Qiang peach belong to the Juglandaceae walnut.Walnut kernel contain grease up to 65%, protein is more than 15.4%, other has phosphatide, multivitamin, and suitable linolic acid, the linolenic acid (food nourishment composition table, People's Health Publisher, 1991) of abundant amount ratio.Walnut oil have very high medical and edibleness (Zhao Shenglan etc., Yunnan University of Traditional Chinese Medicine's journal, 2008,1:61), its preparation method has milling process, water band method, solvent extration etc.In recent years use aqueous enzymatic method and carry out walnut oil extraction (Yi Jianhua etc.: Chinese oil, 2007,2:27; 2007,4:20), the edible vegetable oil yield can reach 40%; 200510046309.5 of patents of invention are directed against decortication Semen Caryae Cathayensis (Juglans mandshurca Maxlm) benevolence and carry out aqueous enzymatic extraction pecan oil; Used enzyme is complex cellulase, proteolytic enzyme etc.; Need 45-60 ℃ enzymolysis 2-10 hour, enzymolysis time is long, is prone to grow mikrobe.The supersonic synergic extraction method only waits the report that Application Research is arranged in other vegetable-protein extraction process in " process for extracting of rice bran polysaccharide and rice bran protein (patent of invention 200710052196.9) ".So far, the water breakthrough enzyme supersonic synergic report that is extracted among walnut oil and the synchronous preparation technology of walnut protein peptide research and uses not as yet.The object of the present invention is to provide the collaborative preparation method of extraction of aqueous enzymatic ultrasonic of walnut oil and walnut protein peptide; This method can be under comparatively gentle condition obtains walnut oil, walnut protein peptide, walnut dietary fiber food through once extracting, and makes that its enzymolysis separation efficiency effectively improves, the enzymolysis and extraction disengaging time shortens greatly.
Summary of the invention
The objective of the invention is to invent a kind of through defibrination, enzymolysis and supersound process, compositions such as walnut oil, walnut protein and walnut polysaccharide starch enzymolysis and breakdown of emulsion from combine closely are discharged; Thereby effectively improve walnut oil and walnut protein extraction efficiency, increase the synchronous aqueous enzymatic ultrasonic extraction of multifarious walnut oil of functional product and walnut protein peptide.Extraction conditions of the present invention is relatively gentle, and the product proterties is good, the walnut residue after the extraction through dry abrasive dust as dietary fiber food.This walnut oil organic solvent-free of gained is residual, can satisfy the green food requirement; Gained walnut protein peptide mixt can further prepare anti-oxidation peptide as inhibitor; Be used for healthcare products, foodstuff additive, nutrition-fortifying agent, animal with additive, makeup, cosmetics of everyday use etc., residue can be used as the walnut dietary fiber food.
The technical scheme that the present invention adopts is: add the water mill slurry in walnut kernel that will remove clothing or powder by solid-to-liquid ratio 1: 5～20; Add proteolytic enzyme or compound protease 1～1,000,000 units/every liter then, 25～60 ℃ of temperature, by every liter of ultrasonication of 10～1500W/ 1～30 minute; Further stir again afterwards and extracted 10～180 minutes; Carry out spinning after extract finishing, oil, protein peptide solution and residue carried out three phase separation, the oil phase that obtains through make with extra care walnut oil.
Described proteolytic enzyme is papoid, bromeline, stomach en-, trypsinase or bacillus protein enzyme.
Said compound protease is that various proteolytic enzyme mix and use, and perhaps one or more in proteolytic enzyme and trichoderma cellulase, αDian Fenmei, βDian Fenmei, the tannase mix use.
Described wet concentration is with pure water, deionized water or zero(ppm) water.
Protein peptide solution directly is used to produce degreasing walnut protein nutrition breast.Or prepare the walnut anti-oxidation peptide through nanofiltration and be used for healthcare products, foodstuff additive, nutrition-fortifying agent, animal with additive, makeup, cosmetics of everyday use etc., or concentrate through vacuum and low temperature and to produce walnut nutritious condensed milk, or spraying drying gets the walnut protein nutritive powder.Described walnut mainly is hulled walnut kernel or walnut powder; Protein peptide solution prepares the walnut anti-oxidation peptide through nanofiltration.Protein peptide solution concentrates through vacuum and low temperature and produces walnut nutritious condensed milk.Protein peptide solution concentrates with spraying drying through vacuum and low temperature and gets the walnut protein nutritive powder.The residue that obtains prepares dietary fiber food through dry abrasive dust.
Described proteolytic enzyme can adopt any proteolytic enzyme that can produce anti-oxidation peptide from substrate; Be generally from biological arbitrarily peptide bond hydrolysis enzyme such as animal, plant, mikrobe; For example papoid (papain), stomach en-(pepsin); And derive from pronase e (pronase E), bacillus proteolytic enzyme (proteasefrom Bacillus) of mikrobe etc. trypsin trypsin); Wherein preferred papoid, stomach en-, trypsinase; These proteolytic enzyme can individually or mix and use, so that shorten hydrolysis time, or in finished product, obtain the better or stronger protein peptide of function of mouthfeel;
Said proteinase activity unified for caseinhydrolysate PM under optimum condition produce trichoroacetic acid(TCA) not the throw out ultraviolet absorption value that is equivalent to 1 microgram tyrosine be a unit.
Said compound protease be various proteolytic enzyme separately or mix, one or more in perhaps various proteolytic enzyme and trichoderma cellulase, αDian Fenmei, βDian Fenmei, the tannase mix use so that raising product yield and quality, the shortening course of processing;
The described enzyme that goes out is treated to and adopts heating means to come inactivated proteases, for example, 90 ℃ of heating 5 minutes, or boils 3 minutes;
The present invention through enzymolysis and supersound process, compositions such as walnut oil, walnut protein enzymolysis breakdown of emulsion from combine closely is discharged; Thereby effectively improve walnut oil and walnut protein peptide extraction efficiency; Compare with traditional milling process, water extraction walnut oil-producing technique, production process equipment is simple, operational safety, energy consumption is lower, comprehensive utilization ratio is high, functional product is many, three-waste free pollution.
The present invention is a new integrated technique, compares with traditional technology, has following tangible advantage:
1. equipment is simple, operational safety; 2. can improve oil yield efficiency, and resulting free oil quality is higher, is easy to refining; 3. enzymatic treatment mild condition; 4. extract through supersonic synergic, the treatment time shortens greatly; 5. easily separated, separate oil phase, water and residue as adopting three-phase centrifuge, can enhance productivity greatly; 6. production process does not have the three wastes, for environmentally friendly.
Embodiment
Embodiment 1 removes clothing walnut kernel 1kg, adds the water of 9kg, and the colloidal mill defibrination adds papoid by 100,000 units/every liter then, and with 1.5kw supersound process 10 minutes, 60 ℃ were continued insulated and stirred extraction 30 minutes.Extraction finishes, and 4000rpm is centrifugal in the back, separates to obtain oil phase 0.45kg, water 8.5kg, residue 0.5kg.The oil phase that obtains is through refining limpid walnut oil 0.4kg, and water then gets walnut protein peptide mixt 0.3kg through concentrating with spraying drying, and residue gets dietary fiber food 0.3kg through dry abrasive dust.
Embodiment 2 gets not de-oiling walnut powder 1kg; Add 5kg water, the colloidal mill defibrination is then by 50,000 proteolytic enzyme units/every liter of compound protease that adds bacillus protein enzyme+αDian Fenmei composition; 10w supersound process 1 minute, 50 ℃ were continued insulated and stirred and extracted 10 minutes.Extraction finishes, and 4000rpm is centrifugal in the back, separates to obtain oil phase 0.5kg, water 8.5kg, residue 0.45kg.The oil phase that obtains is through refining limpid walnut oil 0.45kg, and water then gets walnut protein peptide mixt 0.35kg through concentrating with spraying drying, and the then dry abrasive dust of residue gets dietary fiber food 0.2kg.
Embodiment 3 removes clothing walnut kernel 1kg, adds 20kg water, and the colloidal mill defibrination adds stomach en-to every liter 10,000 unit then, 1kw supersound process 30 minutes, and 25 ℃ are continued insulated and stirred and extracted 180 minutes.Extraction finishes, and 4000rpm is centrifugal in the back, separates to obtain oil phase 0.45kg, water 8.5kg, residue 0.5kg.The oil phase that obtains is through refining limpid walnut oil 0.4kg; Water adopts film filter; Damming, to obtain molecular weight be the component between 200～8000; The freeze-drying drying must be derived from the anti-oxidation peptide mixture 0.05kg of walnut protein then, and other filter component merges concentrated and spraying drying gets instand walnut powder 0.3kg, and the then dry abrasive dust of residue gets dietary fiber food 0.2kg.
Claims (3)
1. walnut oil and extract the preparation method with the synchronous aqueous enzymatic ultrasonic of walnut protein peptide; It is characterized in that getting not de-oiling walnut powder 1kg; Add 5kg water, the colloidal mill defibrination is then by 50,000 proteolytic enzyme units/every liter of compound protease that adds bacillus protein enzyme+αDian Fenmei composition; 10w supersound process 1 minute, 50 ℃ were continued insulated and stirred and extracted 10 minutes; The 4000rpm spinning was carried out three phase separation with oil, protein peptide solution and residue after extraction finished, and the oil phase that obtains is through the purified walnut oil;
Said protein peptide solution prepares the walnut anti-oxidation peptide through nanofiltration; Or
Protein peptide solution concentrates through vacuum and low temperature and produces walnut nutritious condensed milk; Or
Protein peptide solution directly is used to produce degreasing walnut protein nutrition breast; Or
Protein peptide solution concentrates with spraying drying through vacuum and low temperature and gets the walnut protein nutritive powder.
2. the described walnut oil of claim 1 and extract the preparation method with the synchronous aqueous enzymatic ultrasonic of walnut protein peptide,
It is characterized in that described wet concentration is with pure water, deionized water or zero(ppm) water.
3. the described walnut oil of claim 1 and extract the preparation method with the synchronous aqueous enzymatic ultrasonic of walnut protein peptide is characterized in that residue prepares dietary fiber food through dry abrasive dust.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2008100584394A CN101280327B (en) | 2008-05-26 | 2008-05-26 | Synchronous aqueous enzymatic ultrasonic extraction for walnut oil and walnut protein peptide |
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| CN2008100584394A CN101280327B (en) | 2008-05-26 | 2008-05-26 | Synchronous aqueous enzymatic ultrasonic extraction for walnut oil and walnut protein peptide |
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| CN101280327A CN101280327A (en) | 2008-10-08 |
| CN101280327B true CN101280327B (en) | 2012-03-21 |
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