CN102627694A - Method for extracting wheat bran protein - Google Patents
Method for extracting wheat bran protein Download PDFInfo
- Publication number
- CN102627694A CN102627694A CN2012100862038A CN201210086203A CN102627694A CN 102627694 A CN102627694 A CN 102627694A CN 2012100862038 A CN2012100862038 A CN 2012100862038A CN 201210086203 A CN201210086203 A CN 201210086203A CN 102627694 A CN102627694 A CN 102627694A
- Authority
- CN
- China
- Prior art keywords
- wheat bran
- bran protein
- protein extraction
- extraction
- centrifuging
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a method for extracting a wheat bran protein. The method comprises the steps as follows: grinding and sieving wheat bran through a 20-mesh sieve; soaking for 2-3 h in dilute alkali; preprocessing for 15-20 min through beta-glucanase under the conditions that the temperature is 50 DEG C, the material to liquid ratio is 10 to 1, the pH value is 6.5 and the ratio of the glucanase to a substrate is 1.5%, and soaking in the dilute alkali; and when the material to liquid ratio of the wheat bran to the dilute alkali is (1 to 25) to (2 to 8), centrifuging and separating to obtain liquid supernatant, adjusting the pH, centrifuging and separating, depositing, carrying out acid washing, centrifuging, depositing, carrying out size mixing, adjusting the pH to be neutral, and obtaining the wheat bran protein, wherein the rotary speed of centrifuging and separating in the extracting process is 3,500-4,000 r/min, the time is 30-50 min, the pH for alkali extraction is 11-12, the temperature is 45-50 DEG C, the rotary speed is 120 r/min, the time is 60 min and the protein extraction rate can reach 32%.
Description
Technical field
ThisInvention relates to a kind of method of wheat bran protein extraction.
Background technology
Wheat is as one of global farm crop, and Testa Tritici is wheat and processed side product that the powder factory owner wants, and its massfraction is about 15% of a wheat, but in the actual production because the requirement of flower characters, the wheat bran micromicro that obtains up to wheat weigh 30%.About 2 ~ 300,000,000 t of world's year processing wheat bran, China's wheat bran produce per year about about 7000 ~ 8,000 ten thousand t of record, from scooping out with few fodder industry, seldom are used for deep processing and utilize the little height of economic worth again on the domestic sill of this this wheat bran.Yet except containing more glucide, also have egg than horn of plenty from the matter resource in the wheat bran, it contains record and reaches more than 14%.A kind of naturally high-quality egg of wheat bran egg from, nutritive value and biological value all than wheat egg from the height, it has higher lysine content; And contain the necessary multiple amino acids of human body, and therefore more and more cause the interest that people study, wanting one of research object in wherein is exactly the wheat drum head; It is one of large agricultural byproducts resource of China; Development and utilization has a high potential, and at present, the drum head after China's wheat processing directly applies to fodder industry basically; Seldom be used for deep processing and utilization again, economic worth is not high.Yet be rich in food fibre in the drum head, also have comparatively compositions such as rich in protein, fat, oligose, phytic acid and natural antioxidants, simultaneously the wheat bran wide material sources; Cheap; Therefore, the wheat drum head is carried out comprehensive exploitation and utilization, the comprehensive utilization of cereal processed side product is further developed; And order also can increase value-added content of product, thereby improves economic benefit of enterprises and social benefit.
Summary of the invention
One of the object of the invention is realized through following technical scheme:
Wheat bran is through after the pre-treatment, and diluted alkaline soaks, spinning obtains supernatant and regulates spinning behind the pH, and the gained deposition is regulated pH to neutrality through pickling, centrifugation, obtains wheat bran protein at last.
Wheat bran is pulverized, is sieved, and wheat bran is crossed 20 purposes sieve, and the time that diluted alkaline soaks is 2 ~ 3 hours, and the beta-glucanase pre-treatment is at 50 ℃, solid-liquid ratio 10:1; Under the pH6.5 condition, the ratio of enzyme-to-substrate is that 1.5% o'clock pre-treatment 15-20min, diluted alkaline soak, and the solid-liquid ratio of wheat bran and diluted alkaline is 1:25 ~ 28, spinning, supernatant; Transfer pH, spinning, deposition, pickling, centrifugal; Deposition is sized mixing, and transfers pH to neutral, and the wheat bran egg is from matter.Rotating speed is 3500-4000r/min during spinning in the leaching process, time 30-50 minute, the pH11 of alkaline extraction ~ 12,45 ~ 50 ℃ of temperature, rotating speed 120r/min, time 60min, egg from extraction yield can reach 32%.
This method has been dwindled the liquid material ratio of egg from the matter leaching process greatly, has not only saved water consumption, and can improve the raw material processing power; The viscosity of extracting solution reduces greatly, helps follow-up centrifugal separation processes; Egg significantly improves from the matter extraction yield.
Embodiment
Below will combine the preferred embodiments of the present invention to carry out detailed description; Should be appreciated that preferred embodiment has been merely explanation the present invention, rather than in order to limit protection scope of the present invention.
Wheat bran is pulverized, is sieved, and wheat bran is crossed 20 purposes sieve, and the time that diluted alkaline soaks is 2 ~ 3 hours, and the beta-glucanase pre-treatment is at 50 ℃, solid-liquid ratio 10:1; Under the pH6.5 condition, the ratio of enzyme-to-substrate is that 1.5% o'clock pre-treatment 15-20min, diluted alkaline soak, and the solid-liquid ratio of wheat bran and diluted alkaline is 1:25 ~ 28, spinning, supernatant; Transfer pH, spinning, deposition, pickling, centrifugal; Deposition is sized mixing, and transfers pH to neutral, and the wheat bran egg is from matter.Rotating speed is 3500-4000r/min during spinning in the leaching process, time 30-50 minute, the pH11 of alkaline extraction ~ 12,45 ~ 50 ℃ of temperature, rotating speed 120r/min, time 60min, egg from extraction yield can reach 32%.
Claims (6)
1. the method for a wheat bran protein extraction is characterized in that, this method may further comprise the steps: wheat bran is pulverized, is sieved, and beta-glucanase pre-treatment, diluted alkaline soak spinning; Supernatant is transferred pH, spinning, deposition, pickling; Centrifugal, deposition is sized mixing, and transfers pH to neutral, and the wheat bran egg is from matter.
2. the method for a kind of wheat bran protein extraction as claimed in claim 1 is characterized in that: wheat bran is crossed 20 purposes sieve, and the time that diluted alkaline soaks is 2 ~ 3 hours.
3. the method for a kind of wheat bran protein extraction as claimed in claim 1 is characterized in that: at 50 ℃, and solid-liquid ratio 10:1, under the pH6.5 condition, the ratio of enzyme-to-substrate is 1.5% o'clock pre-treatment 15-20min.
4. the method for a kind of wheat bran protein extraction as claimed in claim 1 is characterized in that: the solid-liquid ratio of wheat bran and diluted alkaline is 1:25 ~ 28.
5. the method for a kind of wheat bran protein extraction as claimed in claim 1 is characterized in that: rotating speed is 3500-4000r/min during spinning in the leaching process, time 30-50 minute.
6. the method for a kind of wheat bran protein extraction as claimed in claim 1 is characterized in that: the pH11 of alkaline extraction ~ 12,45 ~ 50 ℃ of temperature, rotating speed 120r/min, time 60min, egg extraction yield certainly can reach 32%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100862038A CN102627694A (en) | 2012-03-29 | 2012-03-29 | Method for extracting wheat bran protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100862038A CN102627694A (en) | 2012-03-29 | 2012-03-29 | Method for extracting wheat bran protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102627694A true CN102627694A (en) | 2012-08-08 |
Family
ID=46586107
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012100862038A Pending CN102627694A (en) | 2012-03-29 | 2012-03-29 | Method for extracting wheat bran protein |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102627694A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103472018A (en) * | 2013-09-27 | 2013-12-25 | 南开大学 | Method for measuring true protein content of corn stalk fermentation feed |
| CN105341314A (en) * | 2015-10-30 | 2016-02-24 | 中国农业科学院作物科学研究所 | Buckwheat bran active protein extraction and separation technology |
| CN107006619A (en) * | 2017-02-21 | 2017-08-04 | 范恒 | A kind of preparation method of whipping additive |
| CN107446979A (en) * | 2017-09-19 | 2017-12-08 | 中国科学院青岛生物能源与过程研究所 | A kind of method of two steps enzymolysis and extraction wheat bran albumen |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1817167A (en) * | 2006-03-07 | 2006-08-16 | 山西大学 | Extraction of buckwheat protein from buckwheat bran |
| CN1875737A (en) * | 2006-06-30 | 2006-12-13 | 北京工商大学 | An oat peptide and extraction method thereof |
| CN101558845A (en) * | 2009-04-16 | 2009-10-21 | 陈福库 | Method for extracting oat starch, protein powder and beta-glucan from oat bran |
-
2012
- 2012-03-29 CN CN2012100862038A patent/CN102627694A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1817167A (en) * | 2006-03-07 | 2006-08-16 | 山西大学 | Extraction of buckwheat protein from buckwheat bran |
| CN1875737A (en) * | 2006-06-30 | 2006-12-13 | 北京工商大学 | An oat peptide and extraction method thereof |
| CN101558845A (en) * | 2009-04-16 | 2009-10-21 | 陈福库 | Method for extracting oat starch, protein powder and beta-glucan from oat bran |
Non-Patent Citations (3)
| Title |
|---|
| 徐忠等: "麦麸蛋白的碱法提取工艺及乳化性质研究", 《食品工业科技》 * |
| 李远坤: "大麦麸皮蛋白的提取及碱水解条件的研究", 《生命科学仪器》 * |
| 管骁等: "碱提酸沉法制备燕麦麸浓缩蛋白的工艺及其改进", 《粮食与饲料工业》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103472018A (en) * | 2013-09-27 | 2013-12-25 | 南开大学 | Method for measuring true protein content of corn stalk fermentation feed |
| CN105341314A (en) * | 2015-10-30 | 2016-02-24 | 中国农业科学院作物科学研究所 | Buckwheat bran active protein extraction and separation technology |
| CN107006619A (en) * | 2017-02-21 | 2017-08-04 | 范恒 | A kind of preparation method of whipping additive |
| CN107446979A (en) * | 2017-09-19 | 2017-12-08 | 中国科学院青岛生物能源与过程研究所 | A kind of method of two steps enzymolysis and extraction wheat bran albumen |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101280327B (en) | Synchronous aqueous enzymatic ultrasonic extraction for walnut oil and walnut protein peptide | |
| CN101455403B (en) | Peanut peptide nutrient food and preparation method thereof | |
| CN103113977B (en) | Method for synchronously preparing peanut oil and peanut peptides through aqueous enzymatic extraction | |
| CN101766282B (en) | Process method for simultaneously extracting soybean function factor and therapeutic factor from bean pulp | |
| CN101948897A (en) | Method for extracting shinyleaf yellowhorn oil, shinyleaf yellowhorn polysaccharide and shinyleaf yellowhorn protolysate peptide from shinyleaf yellowhorn | |
| CN102870953A (en) | Processing method for solubilizing and modifying rice bran protein | |
| CN103445159A (en) | Enzymatic preparation method of all-component ginseng juice | |
| CN101695321A (en) | Method for extracting shinyleaf yellowhorn oil by using aqueous enzymatic extraction | |
| CN103436581A (en) | Method for extracting rapeseed peptides through wet milling combined with enzymic method | |
| CN102516375A (en) | Subcritical water extraction method for high-denaturation rice bran protein | |
| CN102627694A (en) | Method for extracting wheat bran protein | |
| CN108277248A (en) | A kind of method of ultrasonic wave extraction active seabuckthorn polypeptide | |
| CN110615856A (en) | Extraction method of seaweed active ingredients | |
| CN106962590A (en) | A kind of method for preparing edible fungus protein powder | |
| CN103710415A (en) | Method for preparing corn peptide by using corn gluten water | |
| CN104264262A (en) | Method for extracting araboxylan and protein fiber from wheat bran | |
| CN104045685A (en) | Method for extracting proteins by using camellia seed cakes | |
| CN104017847A (en) | Method for preparing protein polypeptide from bacillus fermented sugar residue raw material | |
| CN108244329B (en) | Preparation process of quinoa germ protein powder | |
| CN105175482A (en) | Quinoa protein and extraction method thereof | |
| CN114032274B (en) | Method for preparing sesame peptide by hydrolyzing endogenous protease | |
| CN105154504A (en) | Preparation method of collagen peptide extracted from Moringa | |
| CN102150738A (en) | Method for preparing rice bran protein | |
| CN106432405A (en) | Silkworm pupa protein refining method | |
| CN103652308A (en) | Industrial preparation method of high-protein grape seed dregs |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120808 |