CN1817167A - Extraction of buckwheat protein from buckwheat bran - Google Patents
Extraction of buckwheat protein from buckwheat bran Download PDFInfo
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- CN1817167A CN1817167A CN 200610012482 CN200610012482A CN1817167A CN 1817167 A CN1817167 A CN 1817167A CN 200610012482 CN200610012482 CN 200610012482 CN 200610012482 A CN200610012482 A CN 200610012482A CN 1817167 A CN1817167 A CN 1817167A
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Abstract
A process for extracting the buckwheat protein from the buckwheat bran includes such steps as controlling the content of water in buckwheat, extruding for puffing, extracting in the aqueous solution of NaOH, recovering supernatant, regulating pH value until its isoelectric point of protein, educing out protein, removing filtrate, drying and pulverizing.
Description
Technical field
The present invention relates to a kind of method of deep processing of farm products, specifically belong to a kind of method of from buckwheat bran, extracting buck wheat protein.
Background technology
Buckwheat originates from China, has higher nutritive value and medical value.Buck wheat protein (BWP) is the important activity composition of buckwheat, and medical science has proved now that BWP has to be suppressed and reduce cholesterol in blood, the liver, suppress the body fat savings, suppress gall stone and constipation, prevention hypertension, anti-ageing and improve effect such as muscle antifatigue take place, improve colorectal cancer.BWP demands exploitation urgently as pharmaceutical raw material and food additives.
Buckwheat bran is that buckwheat is sloughed outer cot, remainder after crushing screening is isolated Buckwheat flour again, and protein content is generally about 20% in the buckwheat bran.Buckwheat bran is the quality raw materials that extract BWP, but is underutilized so far.
Also there is the people that tartary buckwheat bran is directly reclaimed albumen (seeing CN02153288) with alkali lixiviate, acid precipitation at present, this method is extracted the too high protein denaturation that not only easily makes of temperature, influences the product function characteristic, and the viscosity of crude protein mother liquor is increased, separation difficulty, loss and energy consumption increase.50 ℃ is the critical-temperatures of sex change significantly of some protein, 10 ℃ of the every risings of temperature, and protein denaturation is quickened 600 times.At high temperature add man-hour, protein will stand racemic, hydrolysis, desulfuration and remove chemical change such as acid amides, and the major part during these change is irreversible, and some variation also may generate poisonous amino acid.According to document announcement, traditional alkali is carried buck wheat protein, and not only recovery rate is low, purity is low, and consuming time, power consumption, poor product quality.
Summary of the invention
The purpose of this invention is to provide a kind of method of extracting buck wheat protein from buckwheat bran, and this method albumen yield is higher, extraction time is short, product purity and albumen quality height.
A kind of method of extracting buck wheat protein from buckwheat bran provided by the invention comprises the steps:
A. get buckwheat bran, control wheat bran water content is used the extrusion device processes, makes material be the graininess of bulk multi-hole.
B. the NaOH extraction with aqueous solution of the material after expanded reclaims supernatant, regulates the pH value to isoelectric points of proteins, separates out buck wheat protein, discards filtrate, and drying and crushing gets final product.
Control buckwheat bran water content is between 8～15% among the described step a; Three grades of temperature of extrusion are respectively 78 ℃, 120 ℃, 160 ℃, screw speed 150～280r/min.
The pH of the used NaOH aqueous solution is between 7～9 among the described step b, and solid-liquid ratio is 1: 5～1: 15, and extracting temperature is 30～50 ℃, and extraction time is 25～45min.
Advantage of the present invention and effect: this invention adopts extruding and puffing technology that the buckwheat bran raw material is handled, overcome the deficiency that the buckwheat bran quality is tight, be difficult for extracting albumen, make the toughness laminated structure of buckwheat bran become loose alveolate texture through extrusion, reached effective broken wall to the material cell membrane, improved the mass transfer characteristic of material cellular content, shorten the dissolution time of buckwheat bran albumen, improved protein extracting ratio.Another advantage of this method is that the protein extraction temperature is low, and not only energy savings be the more important thing is to have overcome proteolysis, desulfuration and removed chemical changes such as acid amides, has guaranteed the quality of product.Reduce the viscosity of crude protein mother liquor simultaneously, reduced the loss of protein in separation process, improved the content and the yield of buck wheat protein in the product.
The specific embodiment:
Embodiment 1
Get the 2.5kg tartary buckwheat bran and add the 0.075L clear water, making the wheat bran water content is 13%, uses the twin-screw extruder extrusion, and technical parameter is: three grades of temperature of extruder transfer to 78 ℃, 120 ℃, 160 ℃ respectively, screw speed 200r/min.The NaOH solution of 25L pH=9 is added in the tartary buckwheat bran after expanded, and 40 ℃ are stirred and extract 25min, and 4500r/min centrifugation 5min collects supernatant and precipitation respectively.Step was extracted once again above precipitation repeated.Twice centrifuged supernatant merges, and the hydrochloric acid that adds concentration 10% is transferred pH value 4.3～4.5, and placement is spent the night, centrifugation (4500r/min, 5min).Precipitation is washed till neutrality with distilled water, and 60 ℃ of vacuum drying are pulverized.Get bitter buckwheat albumen powder 450g, Kjeldahl is surveyed product crude protein content 74.2%, albumen yield 74.1%.
Embodiment 2
Get sweet buckwheat bran 2.5kg, experimental technique and condition are with embodiment 1, and the result gets sweet buckwheat albumen powder 445g, and Kjeldahl is surveyed product crude protein content 72.3%, albumen yield 71.5%.
Claims (4)
1, from buckwheat bran, extracts the method for buck wheat protein, it is characterized in that comprising the steps:
A. get buckwheat bran, control wheat bran water content is used the extrusion device processes, makes material be the graininess of bulk multi-hole.
B. the NaOH extraction with aqueous solution of the material after expanded reclaims supernatant, regulates the pH value to isoelectric points of proteins, separates out buck wheat protein, discards filtrate, and drying and crushing gets final product.
2,, it is characterized in that control buckwheat bran water content is between 8～15% among the step a according to the method for the described extraction buck wheat protein of claim 1.
3,, it is characterized in that three grades of temperature of extrusion are respectively 78 ℃, 120 ℃, 160 ℃ among the step a, screw speed 150～280r/min according to the method for the described extraction buck wheat protein of claim 1.
4, according to the method for the described extraction buck wheat protein of claim 1, the pH that it is characterized in that the used NaOH aqueous solution among the step b is between 7～9, and solid-liquid ratio is 1: 5～1: 15, and extracting temperature is 30～50 ℃, and extraction time is 25～45min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100124828A CN100384339C (en) | 2006-03-07 | 2006-03-07 | Extraction of buckwheat protein from buckwheat bran |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100124828A CN100384339C (en) | 2006-03-07 | 2006-03-07 | Extraction of buckwheat protein from buckwheat bran |
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| Publication Number | Publication Date |
|---|---|
| CN1817167A true CN1817167A (en) | 2006-08-16 |
| CN100384339C CN100384339C (en) | 2008-04-30 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB2006100124828A Expired - Fee Related CN100384339C (en) | 2006-03-07 | 2006-03-07 | Extraction of buckwheat protein from buckwheat bran |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101366443B (en) * | 2008-10-07 | 2010-12-15 | 湖北省云梦龙云蛋白食品有限公司 | Method for preparing wheat separation protein |
| CN102627694A (en) * | 2012-03-29 | 2012-08-08 | 常熟市珍门麦芽糖厂 | Method for extracting wheat bran protein |
| CN105146047A (en) * | 2015-10-19 | 2015-12-16 | 内蒙古清谷新禾有机食品集团有限责任公司 | Method for extracting buckwheat protein from buckwheat |
| CN105341314A (en) * | 2015-10-30 | 2016-02-24 | 中国农业科学院作物科学研究所 | Buckwheat bran active protein extraction and separation technology |
| CN107006674A (en) * | 2017-02-21 | 2017-08-04 | 范恒 | A kind of extraction of gluten protein and method of modifying |
| CN108236714A (en) * | 2018-01-29 | 2018-07-03 | 江苏神华药业有限公司 | Alleviate physical fatigue and assist the composition and its soft capsule of reducing blood lipid |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1502256A (en) * | 2002-11-26 | 2004-06-09 | 北京市粮食科学研究所 | Technology for continuous producing duck wheat protein, flavone and food fibre |
-
2006
- 2006-03-07 CN CNB2006100124828A patent/CN100384339C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101366443B (en) * | 2008-10-07 | 2010-12-15 | 湖北省云梦龙云蛋白食品有限公司 | Method for preparing wheat separation protein |
| CN102627694A (en) * | 2012-03-29 | 2012-08-08 | 常熟市珍门麦芽糖厂 | Method for extracting wheat bran protein |
| CN105146047A (en) * | 2015-10-19 | 2015-12-16 | 内蒙古清谷新禾有机食品集团有限责任公司 | Method for extracting buckwheat protein from buckwheat |
| CN105341314A (en) * | 2015-10-30 | 2016-02-24 | 中国农业科学院作物科学研究所 | Buckwheat bran active protein extraction and separation technology |
| CN107006674A (en) * | 2017-02-21 | 2017-08-04 | 范恒 | A kind of extraction of gluten protein and method of modifying |
| CN108236714A (en) * | 2018-01-29 | 2018-07-03 | 江苏神华药业有限公司 | Alleviate physical fatigue and assist the composition and its soft capsule of reducing blood lipid |
| CN108236714B (en) * | 2018-01-29 | 2021-09-10 | 江苏神华药业有限公司 | Composition for relieving physical fatigue and assisting in reducing blood fat and soft capsule thereof |
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| Publication number | Publication date |
|---|---|
| CN100384339C (en) | 2008-04-30 |
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Granted publication date: 20080430 Termination date: 20110307 |