CN107446979A - A kind of method of two steps enzymolysis and extraction wheat bran albumen - Google Patents
A kind of method of two steps enzymolysis and extraction wheat bran albumen Download PDFInfo
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Abstract
The invention discloses a kind of method of two steps enzymolysis and extraction wheat bran albumen, it is related to bioengineering field.Methods described takes wheat bran slag including (1), adds distilled water and sizes mixing, adjusts pH and temperature, adds breaking-wall cell enzyme reaction and obtains by pretreated slurry；(2) obtained slurry is directly added into distilled water re-started and size mixing, adjust pH and temperature, without other processing, after adding basic protein enzyme reaction 1h 6h, centrifuge, take supernatant and be freeze-dried, you can obtain protein product.Other Enzymatic Extractions are compared, and this method can sufficiently improve the recovery rate of rice bran protein.And especially suitable for the wheat bran after high-temperature process.
Description
Technical field
The present invention relates to bioengineering field, more particularly to a kind of extracting method of wheat bran albumen, more particularly to one kind
The method that two step enzymatic isolation methods extract wheat bran albumen.
Background technology
Accessory substance of the wheat bran as milling industry, annual production are very abundant and cheap.From the point of view of plant histology,
Wheat bran is made up of successively pericarp, kind skin, megarchidium layer and aleurone.And completely can not be separated wheat bran layer during Flour production,
So also contain plumule and endosperm layer except the cortex containing wheat in wheat bran.Thus, generally, contain in wheat bran
12% moisture, 13% albumen, 4% fat and 45% dietary fiber and some other carbohydrate.
For wheat gluten, the albumen in wheat bran contains higher proportion of lysine, arginine, alanine, day
Winter propylhomoserin and glycine and less glutamine, proline, the amino acid of phenylalanine and some sulfur-bearings.Thus, wheat
Albumen in bran has higher nutritive value, can be added in food and some special feeds and improve nutriture value
Value；Wheat bran albumen also has good emulsibility, can apply it in baking goodses and soup stock；Wheat bran albumen is by enzymolysis
Obtained polypeptide can be used as anti-oxidation peptide and antifatigue peptide afterwards；In addition, wheat bran albumen also has freeze proof performance,
It can be added to as the antifreeze of food in food to ensure quality of the food in cryopreservation.
Both at home and abroad extraction wheat bran protein main method for chemical leaching test, Enzymatic Extraction method, ethanol extraction method and
Salt extraction method etc..Chemical leaching test is exactly alkaline extraction, and wheat bran passes through certain under the conditions of alkalescence condition and certain temperature
Centrifuge after time and obtain supernatant, regulation pH to isoelectric point, through centrifugation can obtain sediment you, regulation precipitation
For pH to neutral and then after freeze-drying can obtain wheat bran albumen.Alkalinity extraction is simple to operate, is easier to control
Process conditions processed, extraction efficiency is also higher, thus is applied to large-scale industrial production, but for after High-temperature Liquefaction
For wheat bran slag, because albuminous degeneration is serious, thus it is relatively low with alkalinity extraction rate；Enzymatic Extraction mainly uses protease,
Because protease can be modified and some vegetable proteins of degrading obtain the soluble protein and soluble peptide of some small molecules, and
And enzyme extraction reaction condition is gentle, accessory substance is less, is a kind of method of the current production high-quality protein generally used；Extraction carries
Follow the example of protein in Shi Liyong alcohol steep wheat brans, the impurity contained by the protein of this method extraction is relatively more and extraction process
Cost is higher, thus is not suitable for producing on a large scale.
The content of the invention
For problem present in above-mentioned existing technology, it is an object of the invention to provide a kind of two step enzymolysis and extraction wheats
The method of gliadin, this method is simple to operate, reproducible, can greatly improve the recovery rate and purity of wheat bran albumen.
According to a kind of method of two steps enzymolysis and extraction wheat bran albumen of the present invention, described method comprises the following steps：
(1) the wheat bran slag after removing except starch, adding deionized water and sizing mixing makes wine with dregs concentration in 10%-30%, with quality point
Number adjusts slurry pH to 4.0-7.0 for 5% hydrochloric acid, while adjusts slurry temperature as 40-70 DEG C, total matter based on wheat bran slag
Amount adds Broken kernel enzyme, and Broken kernel enzyme adds the 0.03%-0.3% that quality is wheat bran slag gross mass, after reacting 0.5-5h
Obtain the wheat bran slurry by pretreatment；
(2) add distilled water in the wheat bran slurry by pretreatment obtained to step (1) and size mixing again and be to wine with dregs concentration
4%-25%, and Ph to 6.0-10.0 is adjusted with the sodium hydroxide solution that mass concentration is 4%, it is 55-100 by temperature adjustment
DEG C, alkali protease is added based on wheat bran gross weight, adds the 1.0-3.0% that quality is wheat bran gross weight, reaction 1-5h is laggard
Row centrifuges, and described centrifugal rotational speed is 2000-5000rpm, is freeze-dried after taking supernatant concentration, you can obtain wheat bran
Protein product.
Preferably, the wine with dregs concentration after sizing mixing described in step (1) is 15%；Described pH is 5.5；Described temperature
For 50 DEG C；Described Broken kernel enzyme is the Viscozyme L that Novi believes preparation Co., Ltd, the enzyme amount added be based on
The 0.2% of wheat bran gross weight；The described reaction time is 2.5h.
Preferably, the wine with dregs concentration sized mixing again described in step (2) is 10%；Described pH is 8.0；Described temperature
Spend for 80 DEG C, described alkali protease believes the Alcalase 2.4L FG of preparation Co., Ltd, the enzyme amount added for Novi
For the 2.0% of wheat bran gross weight；Reaction time is 2h, and described centrifugal rotational speed is 4000rpm.
The slurry that the step 1) obtains is handled without other, is directly used in step 2).
The reactants such as alkali or acid need not be added in methods described to be reacted, the alkali includes inorganic base or organic base, institute
Stating acid includes inorganic acid or organic acid.
Above-mentioned " the wheat bran slag after removing except starch " is to add α-amylase liquefaction after being heated to 85-110 DEG C after wheat bran is sized mixing,
And the product obtained after filtering, because the process temperature is higher, the wheat bran slag because obtained from is the wheat after high-temperature process
Bran, this process can remove most of starch in wheat bran.
Beneficial effect
Compared with conventional method, Enzymatic Extraction makes insoluble or albuminate become soluble peptide to be dissolved out, by egg
The polypeptide chain of white matter is hydrolyzed into short peptide chain, improves the digestibility of protein, while reduces the concentration of substrate of reactant, saves
The consumption of alkali and water, the content of solid content in extract solution is improved, remove the energy of extract solution moisture so as to reduce and disappear
Consumption, condition is created for industrial production.
According to the present invention two step enzymolysis and extraction wheat bran methods compared with other Enzymatic Extractions, this method especially suitable for
Wheat bran after high-temperature process because high temperature causes the albuminous degeneration in wheat bran, traditional enzyme process and chemical method be difficult by
Protein extraction therein comes out.The present invention first uses Broken kernel enzyme by the cell of wheat bran before protease extracts wheat bran albumen
Wall is crushed, and is that protein molecular fully exposes, is reused alkali protease and albumen therein is degraded, becoming it can
The polypeptide of dissolubility or small protein molecular, this method can not only increase the recovery rate of wheat bran albumen, and can be modified
Albumen through being denatured, increase its dissolubility.Two enzymes method extracts the industrial biomass high-temperature albuminate of method of wheat bran albumen
Extraction established inositol.
Embodiment
Hereinafter, it will be described in detail the present invention.Before doing so, it should be appreciated that in this specification and appended
Claims in the term that uses should not be construed as being limited to general sense and dictionary meanings, and inventor should allowed
On the basis of appropriate definition term is to carry out the principle of best interpretations, according to implication corresponding with the technical elements of the present invention and generally
Thought explains.Therefore, description presented herein is not intended to limitation originally merely for the sake of the preferred embodiment for illustrating purpose
The scope of invention, it will thus be appreciated that without departing from the spirit and scope of the present invention, it can be obtained by it
His equivalents or improved procedure.
Following examples are enumerated only as the example of embodiment of the present invention, do not form any limit to the present invention
System, it will be appreciated by those skilled in the art that the modification in the range of without departing from the essence of the present invention and design each falls within the present invention
Protection domain.Unless otherwise indicated, the reactant used in following examples is commercially available prod.
Embodiment 1
(1) the wheat bran slag after removing except starch, adding deionized water and sizing mixing makes the wine with dregs concentration be with mass fraction 15%
5% hydrochloric acid regulation slurry pH to 5.5 and temperature is 50 DEG C, and the gross mass based on wheat bran slag adds Broken kernel enzyme, is included in
Measure as 0.2%, the wheat bran slurry by pretreatment is obtained after reacting 2h.
(2) it is 10% that distilled water is added in the slurry for obtaining step (1) to step (1) and is sized mixing again to wine with dregs concentration, and
PH to 8.0 is adjusted with the sodium hydroxide solution that mass concentration is 4%, temperature is set as 80 DEG C, added based on wheat bran gross weight
The Alcalase 2.4L FG of letter preparation Co., Ltd of Novi, addition 2.0%, are centrifuged after reaction 2, described
Centrifugal rotational speed is 4000rpm.It is freeze-dried after taking supernatant concentration, you can obtain wheat bran protein product.
Embodiment 2
In addition to the wine with dregs concentration in step (1) is arranged into 10%.Wheat bran is extracted according to identical method in embodiment 1
Albumen.As a result as shown in table 1, the protein extracting ratio of step (1) is 3%.
Embodiment 3
In addition to the wine with dregs concentration in step (1) is arranged into 20%.Wheat bran is extracted according to identical method in embodiment 1
Albumen.As a result as shown in table 1, the protein extracting ratio of step (1) is 4.7%
Embodiment 4
In addition to the wine with dregs concentration in step (1) is arranged into 25%.Wheat bran is extracted according to identical method in embodiment 1
Albumen.As a result as shown in table 1, the protein extracting ratio of step (1) is 4.1%
Embodiment 5
In addition to the wine with dregs concentration in step (1) is arranged into 30%.Wheat bran is extracted according to identical method in embodiment 1
Albumen.As a result as shown in table 1, the protein extracting ratio of step (1) is 2.6%
It can be seen that the wine with dregs concentration in step (1) has direct shadow to the protein extracting ratio of composite fibre enzyme from the data of table 1
Ring, when wine with dregs concentration is 15%, protein extracting ratio highest, now the hydrolysis result of Broken kernel enzyme is best.
Embodiment 6
Except by addition to temperature is arranged to 55 DEG C in step (2), wheat bran egg is extracted according to identical method in embodiment 1
In vain.As a result as shown in table 2, the protein extracting ratio of step (2) is 32%.
Embodiment 7
Except by addition to temperature is arranged to 65 DEG C in step (2), wheat bran egg is extracted according to identical method in embodiment 1
In vain.As a result as shown in table 2, the protein extracting ratio of step (2) is 41%.
Embodiment 8
Except by addition to temperature is arranged to 75 DEG C in step (2), wheat bran egg is extracted according to identical method in embodiment 1
In vain.As a result as shown in table 2, the protein extracting ratio of step (2) is 45%.
Embodiment 9
Except by addition to temperature is arranged to 85 DEG C in step (2), wheat bran egg is extracted according to identical method in embodiment 1
In vain.As a result as shown in table 2, the protein extracting ratio of step (2) is 64%.
Embodiment 10
Except by addition to temperature is arranged to 90 DEG C in step (2), wheat bran egg is extracted according to identical method in embodiment 1
In vain.As a result as shown in table 2, the protein extracting ratio of step (2) is 62%.
| Wine with dregs concentration | Protein extracting ratio |
| Embodiment 2 | 3% |
| Embodiment 1 | 5.2% |
| Embodiment 3 | 4.7% |
| Embodiment 4 | 4.1% |
| Embodiment 5 | 2.6% |
Table 1
Table 2
| pH | Protein extracting ratio |
| Embodiment 11 | 14% |
| Embodiment 12 | 28% |
| Embodiment 1 | 71% |
| Embodiment 13 | 62% |
| Embodiment 14 | 52% |
Table 3
Embodiment 11
Except by addition to pH is arranged to 6.0 in step (2), wheat bran albumen is extracted according to identical method in embodiment 1.Knot
Fruit is as shown in table 3, and the protein extracting ratio of step (2) is 14%.
Embodiment 12
Except by addition to pH is arranged to 7.0 in step (2), wheat bran albumen is extracted according to identical method in embodiment 1.Knot
Fruit is as shown in table 3, and the protein extracting ratio of step (2) is 28%.
Embodiment 13
Except by addition to pH is arranged to 9.0 in step (2), wheat bran albumen is extracted according to identical method in embodiment 1.Knot
Fruit is as shown in table 3, and the protein extracting ratio of step (2) is 62%.
Embodiment 14
Except by addition to pH is arranged to 10.0 in step (2), wheat bran albumen is extracted according to identical method in embodiment 1.
As a result as shown in table 3, the protein extracting ratio of step (2) is 52%.Comparative example 1
In addition to the raw material in step (1) to be replaced by the wheat bran of unprocessed (without except starch i.e. high-temperature process).
Wheat bran albumen is extracted according to the identical method of embodiment 1.The recovery rate of albumen is 82%.Above-mentioned conclusion proves that this method is handled
The ability of wheat bran without high-temperature process is more or less the same with handling the ability of the wheat bran through high-temperature process.
Comparative example 2
Remove except the wheat bran slag after starch, adding deionized water and sizing mixing makes wine with dregs concentration 15%, and is 4% with mass concentration
Sodium hydroxide solution regulation pH to 1.0, temperature is set as 80 DEG C, is centrifuged after reacting 1h, described centrifugation turns
Speed is 4000rpm.It is freeze-dried after taking supernatant concentration, you can obtain wheat bran protein product, the recovery rate of albumen is 25%.On
Stating result proves：Except the wheat bran slag after starch (i.e. by high-temperature process), the recovery rate that albumen is extracted with this method is tradition side
Nearly 3 times of method recovery rate, this is the change of very big amount, be those skilled in the art learn technical scheme and
It is difficult to what is expected before its technique effect.
Claims (5)
1. a kind of method of albumen in two steps enzymolysis and extraction wheat bran, it is characterised in that：Comprise the following steps：
(1) the wheat bran slag after removing except starch, adding deionized water and sizing mixing makes the wine with dregs concentration be with mass fraction in 10%-30%
5% hydrochloric acid adjusts slurry pH to 4.0-7.0, while adjusts slurry temperature as 40-70 DEG C, and the gross mass based on wheat bran slag adds
Broken kernel enzyme, Broken kernel enzyme add quality be wheat bran slag gross mass 0.03%-0.3%, react 0.5-5h after obtain through
Cross the wheat bran slurry of pretreatment；
(2) it is 4%- to add distilled water in the wheat bran slurry by pretreatment obtained to step (1) and size mixing again to wine with dregs concentration
25%, and Ph to 6.0-10.0 is adjusted with the sodium hydroxide solution that mass concentration is 4%, it is 55-100 DEG C by temperature adjustment, base
Alkali protease is added in wheat bran gross weight, the 1.0-3.0% that quality is wheat bran gross weight is added, is centrifuged after reacting 1-5h
Separation, described centrifugal rotational speed is 2000-5000rpm, is freeze-dried after taking supernatant concentration, you can obtains the production of wheat bran albumen
Product.
2. the method for two steps enzymolysis and extraction wheat bran albumen according to claim 1, it is characterised in that：Described in step (1)
Size mixing after wine with dregs concentration be 15%；Described pH is 5.5；Described temperature is 50 DEG C；Described Broken kernel enzyme is promise
The Viscozyme L of Wei Xin preparations Co., Ltd；The enzyme amount added is 0.2% based on wheat bran gross weight；Described reaction
Time is 2.5h.
3. the method for two steps enzymolysis and extraction wheat bran albumen according to claim 1, it is characterised in that：Described in step (2)
The wine with dregs concentration sized mixing again be 10%；Described pH is 8.0；Described temperature is 80 DEG C；Described alkali protease is promise
The Alcalase 2.4L FG of Wei Xin preparations Co., Ltd；The enzyme amount added is the 2.0% of wheat bran gross weight；Reaction time is
2h；Described centrifugal rotational speed is 4000rpm.
4. the method for two steps enzymolysis and extraction wheat bran albumen according to claim 1, it is characterised in that：The step 1) obtains
Slurry without other handle, be directly used in step 2).
5. the method for two steps enzymolysis and extraction wheat bran albumen according to claim 1, it is characterised in that：Need not in methods described
Add the reactant such as alkali or acid to be reacted, the alkali includes inorganic base or organic base, and the acid includes inorganic acid or organic acid.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108866133A (en) * | 2018-07-12 | 2018-11-23 | 天津科技大学 | Pressure difference pretreatment collaboration high density fermentation wheat bran produces wheat bran oligopeptide method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102627694A (en) * | 2012-03-29 | 2012-08-08 | 常熟市珍门麦芽糖厂 | Method for extracting wheat bran protein |
| CN106858035A (en) * | 2017-04-11 | 2017-06-20 | 中国科学院青岛生物能源与过程研究所 | A kind of method of two steps enzymolysis and extraction rice bran protein |
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2017
- 2017-09-19 CN CN201710851711.3A patent/CN107446979A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102627694A (en) * | 2012-03-29 | 2012-08-08 | 常熟市珍门麦芽糖厂 | Method for extracting wheat bran protein |
| CN106858035A (en) * | 2017-04-11 | 2017-06-20 | 中国科学院青岛生物能源与过程研究所 | A kind of method of two steps enzymolysis and extraction rice bran protein |
Non-Patent Citations (3)
| Title |
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| 杜亚军: "小米糠深加工研究进展", 《粮食与饲料工业》 * |
| 王乔等: "两步酶解提取米糠蛋白的工艺研究及优化", 《氨基酸和生物资源》 * |
| 管骁: "复合多糖酶Viscozyme L在制备燕麦麸分离蛋白中的应用", 《农业工程学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108866133A (en) * | 2018-07-12 | 2018-11-23 | 天津科技大学 | Pressure difference pretreatment collaboration high density fermentation wheat bran produces wheat bran oligopeptide method |
| CN108866133B (en) * | 2018-07-12 | 2022-03-04 | 天津科技大学 | Method for producing wheat bran oligopeptide by virtue of synergistic high-density fermentation of wheat bran through differential pressure pretreatment |
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Application publication date: 20171208 |