CN1875737A - An oat peptide and extraction method thereof - Google Patents
An oat peptide and extraction method thereof Download PDFInfo
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- CN1875737A CN1875737A CNA2006100895252A CN200610089525A CN1875737A CN 1875737 A CN1875737 A CN 1875737A CN A2006100895252 A CNA2006100895252 A CN A2006100895252A CN 200610089525 A CN200610089525 A CN 200610089525A CN 1875737 A CN1875737 A CN 1875737A
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Abstract
The invention discloses a kind of oat peptide and its extractive method. The affere oat peptide of the invention, molecular weight is 1000-3000Da, the invention adopt enzymatic degradation oat husk to obtain oat peptide. The oat peptide extract method that the invention offered comprise the procedure as follows: (1) preconditioning (2) enzymatic treatment (3) filter and meberane treatment to obtain oat peptide. The invention apply enzimatic method to degradate the oat protein, to obtain oat peptide, pass though filter, hyper filtration way to separate the yield, the obtained oat peptide molecular weight is between 1000-3000 Da, absorb easier ; the obtained oat peptide have the activation of restraining skin collagen catabolic enzyme,and com improve the contain valwe of collagen, can apply in food, cosmetic area widely. The operation and method of the invention is easy, the extraction tatio is high, can reach 75%;the pargacation can reach90%.
Description
Technical field
The present invention relates to a kind of biologically active peptide and extracting method thereof, particularly relate to oat peptide and extracting method thereof.
Background technology
Biologically active peptide (Bioactive peptides) is meant the big class peptide that the vital movement to living organism has benifit or has the special physiological activity.Produce by the tissue of organism self or organ, claim the endogenous biologically active peptide the peptide class that physiological regulatory action is arranged itself; But not body self produces, and the peptide matters that is absorbed the back biologically active with the form of peptide then is called Bioactive Peptides (Exogenous Origin BiologicalActivePeptides).
Vegetable protein becomes the main source that people study active peptide with its irreplaceable advantage.Oat, grass family oat family Avena, annual or herbaceos perennial.Cereals nutrient is worth abundant and comprehensive, mineral element content such as its protein, fat, vitamin E, dietary fiber and calcium, magnesium, iron, phosphorus all are higher than other cereal crops, have reducing blood lipid, reduce cholesterol, multiple living function such as hypotensive, hypoglycemic and non-oxidizability.
Summary of the invention
The purpose of this invention is to provide a kind of oat peptide and extracting method thereof.
Oat peptide provided by the present invention, molecular weight is 1000-3000Da, adopts enzymic degradation to extract from oat bran and obtains.
The method of extraction oat peptide provided by the present invention comprises the steps:
1) preliminary treatment: oat bran is added water, regulate pH to 8-10,45-55 ℃ of heat treated;
2) enzyme is handled: adding alkali protease through in the pretreated liquid, at 45-55 ℃ of following stir process 1-5 hour; Then, add papain, at 45-55 ℃ of following stir process 1-5 hour;
3) filter with film and handle: the liquid filtering that will handle through enzyme then, is the milipore filter ultrafiltration of 30000-50000Da with the molecular cut off, removes institute's saliferous and concentrated with the NF membrane nanofiltration of 600-800Da then, obtains oat peptide.
Wherein, step 1) oat bran: the weight ratio of water is 1: 10-20.The step 1) processing time is 2-4 hour.
Step 2) described basic protein enzyme activity unit is 50-200 ten thousand U/g, and consumption is 3-5g alkali protease/100g oat bran.Step 2) unit of activity of described papain is 50-200 ten thousand U/ grams, and consumption is 1-3g papain/100g oat bran.
The described filtration of step 3) adopts flame filter press to filter.Flame filter press also is added with diatomite as filter aid in filtering.
The present invention uses the enzymic degradation avenin becomes oat peptide, by means such as filtration, ultrafiltration it is separated, and gained oat peptide molecular weight is beneficial to absorption more at 1000-3000Da; The gained oat peptide has the activity that suppresses the skin collagen catabolic enzyme, and can promote skin collagen content, can be widely used in fields such as food, cosmetics.The inventive method is simple to operate, and the recovery rate height can reach 75%; Product purity is up to 90%.
Description of drawings
Fig. 1 extracts the process chart of oat peptide for the present invention;
Fig. 2 is the inhibitory action of oat peptide to MMP-1;
Fig. 3 A, Fig. 3 B are respectively that collagen I and collagen I II contain spirogram in the rat skin.
The specific embodiment
The preparation of embodiment 1, oat peptide
The process chart that extracts oat peptide as shown in Figure 1, concrete steps are as follows:
1, oat bran is handled:
For more acquisition oat peptides, oat bran selects for use 20 orders to the composition between 60 orders, and Kjeldahl measures wherein that protein content can reach 20%.
2, preliminary treatment:
Oat bran and deionized water were mixed by weight 1: 10, and adjust pH is heated to 45-55 ℃ to about the 8-10, evenly stirs 2-4 hour.
3, enzyme is handled:
Measure liquid pH value after the preliminary treatment, about 8.0-8.5 adds alkali protease, and its unit of activity is 1,000,000 U/g, and consumption is 5g alkali protease/100g oat bran, continues to stir under 45-55 ℃ of temperature and heats 2 hours; Add papain then, its unit of activity 1,000,000 U/ gram, consumption 1g papain/100g oat bran, 55 ℃ are continued stirring and heated 1 hour.
4, utilize flame filter press to filter, add diatomite and help filter, obtain brown supernatant liquid.
5, be the milipore filter ultrafiltration of 50000Da with the molecular cut off, remove big molecule carbohydrate, get and leach liquid.
6, molecular cut off is NF membrane nanofiltration removal institute's saliferous of 800Da and concentrates, and obtains oat peptide.
The gained oat peptide is a kind of hybrid peptide, and its molecular weight is 1000-3000Da, and product purity is 90%, and recovery rate is 75%.
The functional experiment of embodiment 2, oat peptide
1, for the activity inhibition of MMP-1 (Matrix Metalloproteinase)
In vitro, the prepared oat peptide of test implementation example 1 is to the activity inhibition of skin collagen catabolic enzyme MMP-1 (MatrixMetalloproteinase), and the result as shown in Figure 2.The result shows that oat peptide has the inhibition activity of skin collagen catabolic enzyme, and this inhibition is active relevant with concentration.
2, oat peptide can increase the content of rat skin collagen
2.1 animal used as test
40 of cleaning level Wistar rats, male, body weight 220～240g is available from Beijing dimension tonneau China Experimental Animal Center.
2.2 instrument and reagent
New Technique Application Inst., Beijing City MB-ELIASA; Collagen I, collagen I II detection kit are available from Beijing ring Ya Taike biomedical technology Co., Ltd.
2.3 modeling and medication
Rat is divided into normal group and oat peptide group at random, 8 every group, pushes away respectively in the depilation of the ridge both sides of experimental animal rat depilation scope 3cm * 3cm with pet.
During the experiment beginning, do not have the hair-fields in the both sides, back and clean with clear water earlier, choose every rat back both sides then and do not have the middle skin in hair-fields, about 2cm * the 2cm of experimental point, normal group is left intact except that clear water cleans, and the oat peptide group is coated with isodose oat peptide preparation, once a day.Drew materials in 7 days behind the coating.
2.4 index detects
Get animal used as test immediately behind the execution rat and smear position skin, mark right side 2cm * 2cm goes in ring and gets skin, after removing fat, precision weighs weight in wet base, skin is cut for a short time with ophthalmology shred, by 1: 10 (the 1g skin histology adds 10mL physiological saline), move in the glass homogenizer homogenate, constantly cooling in the homogenate process, at 4 ℃ of centrifugal 10min of following 10000rpm, get 100 μ L homogenate supernatant-20 ℃ preservations and be used in reference to target mensuration.
Collagen I assay: adopt the ELASA method to measure collagen I content in the skin.
Collagen I II assay: adopt the ELASA method to measure collagen I II content in the skin.
The result is shown in table 1 and table 2 and Fig. 3 A and Fig. 3 B.
The variation of collagen I content in table 1 rat skin (x ± s)
| Group | Number of animals (n) | Collagen I (ng/ml) |
| Normal group avenabeta glucosan group | 8 8 | 203.87±42.45 462.12±139.39**## |
Compare with normal group, * P＜0.05, #P＜0.05, ##P＜0.01 are compared with control group in * * P＜0.01
The variation of collagen I II content in table 2 rat skin (x ± s)
| Group | Number of animals (n) (ng/ml) | Collagen I II |
| Normal group oat peptide group | 8 8 | 13.88±2.59## 20.80±2.82** |
Compare with normal group, * P＜0.05, #P＜0.05, ##P＜0.0l are compared with control group in * * P＜0.01
The result shows that the collagen I of oat peptide group rat skin and collagen I II content are apparently higher than normal group, and significant difference, shows that the oat Toplink improves the skin surface collagen content, can be used for fields such as food, cosmetics.
Claims (9)
1, a kind of oat peptide, molecular weight is 1000-3000Da, adopts enzymic degradation to extract from oat bran and obtains.
2, oat peptide according to claim 1 is characterized in that: described Enzymatic Extraction comprises the steps:
1) preliminary treatment: oat bran is added water, regulate pH to 8-10,45-55 ℃ of heat treated;
2) enzyme is handled: adding alkali protease through in the pretreated liquid, at 45-55 ℃ of following stir process 1-5 hour; Then, add papain, at 45-55 ℃ of following stir process 1-5 hour;
3) filter with film and handle: the liquid filtering that will handle through enzyme then, is the milipore filter ultrafiltration of 30000-50000Da with the molecular cut off, is that institute's saliferous and concentrated is removed in the NF membrane nanofiltration of 600-800Da then with the molecular cut off, obtains oat peptide.
3, a kind of method of extracting oat peptide comprises the steps:
1) preliminary treatment: oat bran is added water, regulate pH to 8-10,45-55 ℃ of heat treated;
2) enzyme is handled: adding alkali protease through in the pretreated liquid, at 45-55 ℃ of following stir process 1-5 hour; Then, add papain, at 45-55 ℃ of following stir process 1-5 hour;
3) filter with film and handle: the liquid filtering that will handle through enzyme then, is the milipore filter ultrafiltration of 30000-50000Da with the molecular cut off, is that institute's saliferous and concentrated is removed in the NF membrane nanofiltration of 600-800Da then with the molecular cut off, obtains oat peptide.
4, method according to claim 3 is characterized in that: the step 1) oat bran: the weight ratio of water is 1: 10-20.
5, method according to claim 3 is characterized in that: the step 1) processing time is 2-4 hour.
6, according to the arbitrary described method of claim 3-5, it is characterized in that: step 2) described basic protein enzyme activity unit is 50-200 ten thousand U/g, consumption is 3-5g alkali protease/100g oat bran.
7, according to the arbitrary described method of claim 3-5, it is characterized in that: step 2) unit of activity of described papain is 50-200 ten thousand U/ grams, consumption is 1-3g papain/100g oat bran.
8, according to the arbitrary described method of claim 3-5, it is characterized in that: the described filtration of step 3) adopts flame filter press to filter.
9, method according to claim 8 is characterized in that: flame filter press also is added with diatomite as filter aid in filtering.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101884783A (en) * | 2010-07-08 | 2010-11-17 | 辽宁大学 | Wheat bran polypeptide-containing anti-fatigue traditional Chinese medicine and preparation method thereof |
| CN101117642B (en) * | 2007-07-31 | 2011-02-09 | 北京工商大学 | Polypeptide capable of lowering blood pressure and method for preparing same |
| CN102198057A (en) * | 2010-03-22 | 2011-09-28 | 北京工商大学 | Oat extract, preparation method thereof and application thereof |
| CN102349579A (en) * | 2011-09-28 | 2012-02-15 | 广州合诚实业有限公司 | Oat peptide milk and preparation method thereof |
| CN102627694A (en) * | 2012-03-29 | 2012-08-08 | 常熟市珍门麦芽糖厂 | Method for extracting wheat bran protein |
| CN113699208A (en) * | 2021-09-07 | 2021-11-26 | 三主粮集团股份公司 | Oat peptide and preparation method thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE871364A (en) * | 1977-10-18 | 1979-02-15 | Lyckeby Staerkelsefoeraedling | PROCESS FOR THE PREPARATION OF A WHOLE GRAIN HYDROLYSIS PRODUCT AND PRODUCT THUS OBTAINED |
| CN101139625B (en) * | 2002-12-24 | 2012-11-28 | 加拿大农业及农业食品部 | Ace inhibitory peptides from plant materials |
| TWI328457B (en) * | 2003-03-18 | 2010-08-11 | Suntory Holdings Ltd | Angiotensin-converting enzyme inhibitory peptides |
-
2006
- 2006-06-30 CN CNB2006100895252A patent/CN100456948C/en active Active
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101117642B (en) * | 2007-07-31 | 2011-02-09 | 北京工商大学 | Polypeptide capable of lowering blood pressure and method for preparing same |
| CN102198057A (en) * | 2010-03-22 | 2011-09-28 | 北京工商大学 | Oat extract, preparation method thereof and application thereof |
| CN102198057B (en) * | 2010-03-22 | 2012-09-19 | 北京工商大学 | Oat extract, preparation method thereof and application thereof |
| CN101884783A (en) * | 2010-07-08 | 2010-11-17 | 辽宁大学 | Wheat bran polypeptide-containing anti-fatigue traditional Chinese medicine and preparation method thereof |
| CN101884783B (en) * | 2010-07-08 | 2013-03-06 | 辽宁大学 | Wheat bran polypeptide-containing anti-fatigue traditional Chinese medicine and preparation method thereof |
| CN102349579A (en) * | 2011-09-28 | 2012-02-15 | 广州合诚实业有限公司 | Oat peptide milk and preparation method thereof |
| CN102349579B (en) * | 2011-09-28 | 2013-04-17 | 广州合诚实业有限公司 | Oat peptide milk and preparation method thereof |
| CN102627694A (en) * | 2012-03-29 | 2012-08-08 | 常熟市珍门麦芽糖厂 | Method for extracting wheat bran protein |
| CN113699208A (en) * | 2021-09-07 | 2021-11-26 | 三主粮集团股份公司 | Oat peptide and preparation method thereof |
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| CN100456948C (en) | 2009-02-04 |
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Owner name: BEIJING SANYOU HUIZHI BIOTECHNOLOGY CO.LTD. Free format text: FORMER OWNER: BEIJING TECHNOLOGY AND BUSINESS UNIEERSITY Effective date: 20070518 |
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Effective date of registration: 20070518 Address after: 102200, No. 5, super Road, Changping District Economic Development Zone, Beijing Applicant after: Beijing Sanyou Huizhi Biological Technology Co., Ltd. Address before: 100037, Fu Cheng Road, Beijing, Haidian District, No. 33 Applicant before: Beijing Technology and Business University |
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