CN102198057A - Oat extract, preparation method thereof and application thereof - Google Patents
Oat extract, preparation method thereof and application thereof Download PDFInfo
- Publication number
- CN102198057A CN102198057A CN 201010130697 CN201010130697A CN102198057A CN 102198057 A CN102198057 A CN 102198057A CN 201010130697 CN201010130697 CN 201010130697 CN 201010130697 A CN201010130697 A CN 201010130697A CN 102198057 A CN102198057 A CN 102198057A
- Authority
- CN
- China
- Prior art keywords
- oat
- oat extract
- enzyme
- liquid
- amylase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Cosmetics (AREA)
Abstract
The invention discloses an oat extract, a preparation method and an application thereof. The preparation method of the oat extract comprises: 1) putting oat malts into water, adjusting pH value within a range of 9 to 11, and heating for 1 to 3 hours at a temperature of from 70 to 80 DEG C, 2) cooling and centrifuging the liquid obtained from the above step, collecting a supernatant fluid, adjusting pH value of the supernatant fluid within a range of 5 to 6, adding a diastase into the supernatant fluid for an enzymolysis reaction, leaching after the enzymolysis reaction, collecting a leachate, and carrying out an enzyme inactivation process for the leachate to obtain the oat extract. The oat extract prepared through the method of the invention has the advantages of no needs of organic reagents, convenient extraction and separation method, and good product qualities. Results from a detection for moisturizing effects shows that the oat extract prepared through the method of the invention has a good water lock performance and a long-acting moisturizing effect.
Description
Technical field
The present invention relates to a kind of oat extract and preparation method thereof and application.
Background technology
In the process that oat seed germinates, growth and decline appear in its nutrient composition content to be changed, be vital seed to be risen to from the static state of dormancy be the frequent dynamic process of physiological activity (natural excite the skin process of waking up) because germinate, this moment, the Repiration of seed strengthened, the kind and the quantity of enzyme significantly increase, the adding the metabolism of forcing seed and can on higher level, carry out of enzymatic activity, start a large amount of enzymatic reactions simultaneously and carry out biotransformation, the various materials that store in the seed are transformed and are promoted the high activity composition into supplying new life to utilize, bioactive substance content is significantly increased, and may produce new high activity material.
Summary of the invention
The purpose of this invention is to provide a kind of oat extract and preparation method thereof.
Oat extract provided by the present invention is to prepare according to the method that comprises the steps:
1) oat malt is added in the entry, and regulate pH value, heated 1-3 hour down at 70-80 ℃ to 9-11;
2) with the liquid cools behind the heat treatment, centrifugal, collect supernatant; The pH value of regulating described supernatant is 5-6, adds amylase then and carry out enzyme digestion reaction in described supernatant, and after reaction finished, sucking filtration was collected sucking filtration liquid, described sucking filtration liquid is carried out enzyme denaturing handle, and obtained described oat extract.
Wherein, the mass ratio of oat malt described in the step 1) and water can be 1: 20-30 is preferably 1: 20.
Step 2) amylase described in is heatproof amylase, and as the letter heatproof amylase of Novo RPS Ulc, standard activity is 120KNU/g (the 80000U/ gram that is equivalent to GB).The enzyme activity definition: 1ml enzyme liquid is at 70 ℃, and under the pH6.0 condition, 1 minute liquefaction soluble starch 1mg becomes the needed enzyme amount of dextrin and is an enzyme activity unit.
Heatproof amylase is a kind of liquid enzyme formulation, contains the extremely strong α-Dian Fenmei of heat stability, and utilizing bacillus (Bacillus) expression of genetic modification and making the diastatic specific name of heatproof by base is 1,4-α-D type glucan hydrolase (EC3.2.1.1).Heatproof amylase is a kind of novel Ye Huamei, compares with traditional heat-resistant alpha-amylase, and it can be worked under the lower condition of pH value and calcium content, thereby reduces production costs.
The reaction temperature of described enzyme digestion reaction can be 60-80 ℃, is preferably 80 ℃; The response time of described enzyme digestion reaction can be 30-60 minute, is preferably 40 minutes; In the described enzyme digestion reaction, diastatic addition can be the 100-160U/g oat malt, preferably adds the 140-150U/g oat malt.
Step 2) finishing temperature with the liquid cools behind the heat treatment in is specially 45-50 ℃, preferred 50 ℃; Step 2) centrifugal rotation speed described in is 4000-5000rpm, preferred 4000rpm; The centrifugal time can be 10-15 minute, preferred 10 minutes.
When the extracting solution behind the enzymolysis is carried out sucking filtration,, improve the rate of filtration and clarity, can adopt filter aid kieselguhr to carry out sucking filtration in order to reduce filtration resistance.
A further object of the present invention provides the application of above-mentioned oat extract.
The application of oat extract provided by the present invention is its application in the preparation cosmetics; Especially the application in the suitable type cosmetics of gentle, non-stimulated, the baby of preparation.
The cosmetics that contain oat extract of the present invention also belong to protection scope of the present invention.
The invention provides the preparation method of oat extract, this method does not need organic reagent, and extraction separation method is easy, and the products obtained therefrom quality is better.Since avenin itself have gentle non-stimulated, promote body metabolism, wake skin up, help effect such as cell regeneration, skin care is preserved moisture so its extract can be used as, gentle, infant series additive, in the preparation cosmetics, especially prepare that skin care is preserved moisture, the cosmetics of baby's series, have extensive use.Oat extract of the present invention is carried out moisture-keeping efficacy detect, the result shows that this oat extract has good lock aqueous energy, can long-actingly preserve moisture.
Description of drawings
Fig. 1 is the technological process of production figure of oat extract provided by the invention.
Fig. 2 is the erythrocyte hemolysis rate bar diagram of the oat extract of variable concentrations.
The water content that Fig. 3 makes skin surface respectively for trehalose and oat extract is curve over time.
Fig. 4 makes moisture of skin windage curve over time respectively for trehalose and oat extract.
The specific embodiment
Below by specific embodiment method of the present invention is described, but the present invention does not limit to therewith.Experimental technique described in the following embodiment if no special instructions, is conventional method; Described reagent and material if no special instructions, all can obtain from commercial channels.
The technological process of production figure of oat extract as shown in Figure 1, concrete preparation process is as follows:
Got solid-liquid ratio 1: 20, promptly dried oat malt 50 grams add the 1000mL deionized water; Reaction 120min when adjust pH to 10, agitating heating are warming up to 80 ℃ (add a cover preservative film, reduce evaporation); Stop heating, water cycle is cooled to 50 ℃, under the rotating speed of 4000rpm centrifugal 10 minutes, gets supernatant; The pH value of regulating described supernatant with citric acid is 5～6, add supernatant quality 0.01% amylase (140-150U/g oat malt) (Novi's letter heatproof amylase, standard activity is 120KNU/g (the 80000U/ gram that is equivalent to GB)), 80 ℃ of stirring reaction 40min; The kieselguhr sucking filtration is measured and to be taken out pH value of filtrate, solid content, electrical conductivity, and measurement result is: pH value is 4.5～5.5, solid content be 1～2% and electrical conductivity be 1000～1600 μ s/cm
295 ℃ are incubated 30 minutes afterwards, and water-bath is cooled to 50 ℃, add 0.6%LGP liquid preservative (this antiseptic full name is LIQUID GERMALL PLUS, purchases the international special product company limited in ISP), obtain product.Afterwards as required, can carry out subsequent operation, as cask washing, sterilization, fill, sign indicating number is beaten in vanning, warehouse-in.
The functional examination test of embodiment 2, oat extract
1, irritation test
Zest size by erythrocyte hemolysis test evaluation oat extract.
(1) test principle
The zest of raw material can be weighed by the stimulation degree size to erythrocyte, measures the absorbance of raw material under wavelength 530nm, and absorbance is big more, illustrates that the cell hemolysis rate is high more, and the zest of raw material is high more.
(2) test method
The preparation and the storage of erythrocyte (RBC):
Get fresh SANHUANG Sanguis Gallus domesticus 10mL and 5mL citrate buffer solution mixing, be incubated immediately in 21-22 ℃ of couveuse.
The separation of RBC: under the room temperature, at 1500rpm centrifugal 15 minutes, draw supernatant and discard.Shuffle RBC in the centrifuge tube, triplicate with equivalent PBS buffer.
Add glucose to RBC, making the glucose final concentration is 10mmol/L, and 4 ℃ of sealings are preserved.Before the experiment, its hygral equilibrium is to room temperature.
The hemolytic experiment of functional component: in the EP pipe, add sample according to the sample size in the table 1 respectively, each pipe is placed shaking table, after 150rpm, 37 ℃ are hatched 10 minutes, centrifugal 1 minute of 10000rpm, getting supernatant and survey absorbance (OD value) under 530nm, is blank with PBS.
Table 1 functional component hemolytic experiment reagent dosage table
The results are shown in Figure 2, as can be seen, compare with contrast 0.1%SDS solution, the basic pair cell not damaged of oat extract, hemolysis rate is extremely low, shows that oat extract is gentle, to the basic nonirritant of skin.
2, moisture-keeping efficacy detects
Adopt human test's mode,, choose 20 volunteers with the water content and TEWL (epidermis loss of water) value of moisture tester test skin; Volunteer men and women half and half.
Adopt two kinds of solution to be coated on skin surface: a kind of solution is for containing the aqueous trehalose of 2.0% (mass percent concentration), and another kind of solution is that the oat extract solution of 2ml embodiment 1 preparation is dissolved in the aqueous solution that obtains in the 98ml water; Test once per half an hour, totally 4 hours.The water content percentage change of skin surface over time curve as shown in Figure 3, the result shows: compare with trehalose, provided by the invention is that the skin moistening additive of main component has better moistening effect with the avenin.Moisture of skin windage percentage change over time curve as shown in Figure 4, the result shows: with the contrast trehalose compare, oat extract provided by the invention has good lock aqueous energy, can long-actingly preserve moisture.
Claims (10)
1. the preparation method of an oat extract comprises the steps:
1) oat malt is added in the entry, and regulate pH value, heated 1-3 hour down at 70-80 ℃ to 9-11;
2) with the liquid cools behind the heat treatment, centrifugal, collect supernatant; The pH value of regulating described supernatant is 5-6, adds amylase then and carry out enzyme digestion reaction in described supernatant, and after reaction finished, sucking filtration was collected sucking filtration liquid, described sucking filtration liquid is carried out enzyme denaturing handle, and obtained described oat extract.
2. method according to claim 1 is characterized in that: the mass ratio of oat malt described in the step 1) and water is 1: 20-30 is preferably 1: 20.
3. method according to claim 1 and 2 is characterized in that: in the step 1) oat malt is added in the entry, and regulate pH value to 10,80 ℃ of following heat treated 2 hours.
4. according to arbitrary described method among the claim 1-3, it is characterized in that: described amylase is heatproof amylase; Described heatproof amylase is liquid enzyme formulation, and described heatproof amylase enzyme activity is defined as: 1ml enzyme liquid is at 70 ℃, and under the pH6.0 condition, liquefaction soluble starch 1mg became the needed enzyme amount of dextrin, i.e. an enzyme activity unit in 1 minute.
5. according to arbitrary described method among the claim 1-4, it is characterized in that: step 2) described in the reaction temperature of enzyme digestion reaction be 60-80 ℃, be preferably 80 ℃; The response time of described enzyme digestion reaction is 30-60 minute, is preferably 40 minutes; In the described enzyme digestion reaction, diastatic addition is the 100-160U/g oat malt, preferably adds the 140-150U/g oat malt.
6. according to arbitrary described method among the claim 1-5, it is characterized in that: step 2) in be 45-50 ℃ with the finishing temperature of the liquid cools behind the heat treatment, be preferably 50 ℃; Step 2) centrifugal rotation speed described in is 4000-5000rpm, is preferably 4000r/min; The centrifugal time is 10-15 minute, is preferably 10 minutes.
7. according to arbitrary described method among the claim 1-6, it is characterized in that: step 2) adopt in the described sucking filtration filter aid kieselguhr to carry out sucking filtration.
8. the oat extract that arbitrary described method prepares among the claim 1-7.
9. the application of the described oat extract of claim 8 in the preparation cosmetics.
10. cosmetics is characterized in that: contain the described oat extract of claim 8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101306976A CN102198057B (en) | 2010-03-22 | 2010-03-22 | Oat extract, preparation method thereof and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101306976A CN102198057B (en) | 2010-03-22 | 2010-03-22 | Oat extract, preparation method thereof and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102198057A true CN102198057A (en) | 2011-09-28 |
| CN102198057B CN102198057B (en) | 2012-09-19 |
Family
ID=44659217
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101306976A Active CN102198057B (en) | 2010-03-22 | 2010-03-22 | Oat extract, preparation method thereof and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102198057B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102342901A (en) * | 2011-10-14 | 2012-02-08 | 中华全国供销合作总社南京野生植物综合利用研究所 | Bath cream containing plant protein peptide |
| CN105377054A (en) * | 2013-03-04 | 2016-03-02 | 桂格燕麦公司 | Method of processing oats to achieve oats with an increased avenanthramide content |
| CN106236690A (en) * | 2016-08-31 | 2016-12-21 | 福建省梦娇兰日用化学品有限公司 | A kind of amino acid cleansing cream containing various plants composition |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5888515A (en) * | 1997-12-11 | 1999-03-30 | Albros, L.P. | Rhus dermatitis treatment composition and method |
| CN1875737A (en) * | 2006-06-30 | 2006-12-13 | 北京工商大学 | An oat peptide and extraction method thereof |
| CN101229109A (en) * | 2007-12-12 | 2008-07-30 | 天津盛世恒安科技发展有限公司 | Skin protectant suitable for 7-12 months old infant skin and preparing process thereof |
| CN101338247A (en) * | 2008-08-12 | 2009-01-07 | 北京工商大学 | Oat oil, extracting process thereof and applications |
-
2010
- 2010-03-22 CN CN2010101306976A patent/CN102198057B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5888515A (en) * | 1997-12-11 | 1999-03-30 | Albros, L.P. | Rhus dermatitis treatment composition and method |
| CN1875737A (en) * | 2006-06-30 | 2006-12-13 | 北京工商大学 | An oat peptide and extraction method thereof |
| CN101229109A (en) * | 2007-12-12 | 2008-07-30 | 天津盛世恒安科技发展有限公司 | Skin protectant suitable for 7-12 months old infant skin and preparing process thereof |
| CN101338247A (en) * | 2008-08-12 | 2009-01-07 | 北京工商大学 | Oat oil, extracting process thereof and applications |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102342901A (en) * | 2011-10-14 | 2012-02-08 | 中华全国供销合作总社南京野生植物综合利用研究所 | Bath cream containing plant protein peptide |
| CN102342901B (en) * | 2011-10-14 | 2013-03-20 | 中华全国供销合作总社南京野生植物综合利用研究所 | Bath cream containing plant protein |
| CN105377054A (en) * | 2013-03-04 | 2016-03-02 | 桂格燕麦公司 | Method of processing oats to achieve oats with an increased avenanthramide content |
| CN106236690A (en) * | 2016-08-31 | 2016-12-21 | 福建省梦娇兰日用化学品有限公司 | A kind of amino acid cleansing cream containing various plants composition |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102198057B (en) | 2012-09-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107446825B (en) | Tremella fuciformis strain and application thereof | |
| CN104523814B (en) | A kind of method for industrializing alpha amylase inhibitor in extraction navy bean | |
| CN104152514A (en) | Origin fermentation method and origin fermentation product prepared by same | |
| CN108823261A (en) | A kind of Ultra-low molecular weight Dendrobium officinale polysaccharide and its preparation and application | |
| CN104257598A (en) | Chinese wolfberry fermented raw stock cosmetic and preparation method thereof | |
| CN108324633A (en) | A kind of preparation method and products thereof of fermentative brown rice stoste used for cosmetic | |
| CN106387923A (en) | Soluble dietary fibers rich in galactomannan and preparation method of soluble dietary fibers | |
| CN106722943B (en) | Apple enzyme and preparation method thereof | |
| CN115125153B (en) | Preparation method and application of galactose yeast-like bacteria fermentation product filtrate | |
| CN109852598A (en) | A kind of method of bean dregs and bafillus natto mixed fermentation producing enzyme | |
| CN102198057B (en) | Oat extract, preparation method thereof and application thereof | |
| CN109939059B (en) | Rice germ five-bacterium fermentation slow-release cosmetic and preparation method and application thereof | |
| CN106511180A (en) | Codonopsis pilosula fermented raw stock cosmetic, preparation method thereof and application thereof | |
| CN108998437A (en) | A kind of Nattokinase liquid state fermentation method | |
| CN106636252B (en) | Wizened bacterium exopolysaccharide and its preparation method and application | |
| CN109330929B (en) | Preparation method of tremella fermentation extract and application of tremella fermentation extract in cosmetics | |
| CN109806201A (en) | A kind of preparation method and application of rose fermentation liquid | |
| CN106755184B (en) | Thelephora ganbajun mycelium polysaccharide and its preparation method and application | |
| CN104498360B (en) | A kind of lactobacillus paracasei active bacteria formulation normal-temperature vacuum drying protectant and its application process | |
| CN112522114B (en) | Cordyceps militaris fungus chaff extract, ganoderma lucidum fermentation product, and preparation methods and applications thereof | |
| CN101530205A (en) | Method for preparing synbiotics by using bacillus natto to ferment rice bran and application | |
| CN111690705A (en) | Preparation method of tussah pupa cordyceps militaris bioactive peptide | |
| CN117511827B (en) | A folium Platycladi fermentation product for caring scalp, controlling oil and removing dandruff, and its preparation method and application | |
| CN111321043A (en) | Preparation method of brown rice fermentation liquor with low alcohol content and product thereof | |
| CN109938338A (en) | A kind of high-performance "Hami" melon ferment and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20170822 Address after: 101200, No. 3, M2-5 District, Hing Valley Economic Development Zone, Pinggu District, Beijing Patentee after: Beijing Orient Miao Sen bio tech ltd Address before: 100037 Beijing city Haidian District Fuchengmen Road No. 33 Patentee before: Beijing Technology and Business University |
|
| TR01 | Transfer of patent right |