CN106566860A - Method for preparing high-purity rice protein and rice polypeptide from rice residues - Google Patents
Method for preparing high-purity rice protein and rice polypeptide from rice residues Download PDFInfo
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- CN106566860A CN106566860A CN201610995810.4A CN201610995810A CN106566860A CN 106566860 A CN106566860 A CN 106566860A CN 201610995810 A CN201610995810 A CN 201610995810A CN 106566860 A CN106566860 A CN 106566860A
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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Abstract
The invention discloses a method for preparing high-purity rice protein and rice polypeptide from rice residues. The method comprises the following steps of preprocessing raw materials: adding hot water to the rice residues generated in a process of producing sugar from rice, performing washing, filtering, dewatering, desanding and accurate grinding, and adjusting slurry concentration; performing an enzymolysis reaction: adjusting the pH value of rice residue slurry by using alkaline water, adding a complex enzyme, and performing the reaction at 58-62 DEG C for 1 hour; performing water-washing and filtering: performing washing separation on the rice residue slurry, performing washing with water until the soluble solid content is within 1%, and collecting a heavy phase component, namely, a crude rice protein; performing protein preparation: sterilizing the obtained crude rice protein at a high temperature, performing dewatering and drying, and performing degreasing and grinding by adopting subcritical extraction equipment to obtain finished rice protein; and performing polypeptide preparation: performing enzymolysis on the obtained finished rice protein as a raw material for 24 hours, performing centrifugal separation, and performing spray drying to obtain finished rice polypeptide. The preparation equipment and process are relatively simple; the yield and content of rice polypeptide are greatly increased; and actual usage requirements are met.
Description
Technical field
The present invention relates to a kind of method that utilization Solid state prepares high-purity rice protein and big mpd polypeptide, belongs to food and adds
Work technical field.
Background technology
, compared with other corn gluten proteins, biological value and protein score, PS are of a relatively high for rice protein, and the necessary ammonia of rice protein
Base acid Compositional balance rationally, meets the idealized model of WHO/FAO recommendations, and its business value can be with the animal such as egg, beef, milk
Albumen compares favourably.Therefore, rice protein is the quality plant albumen recognized by people;In addition, the low antigenicity of rice protein
It is its another outstanding feature, this is highly beneficial to infant.True digestibility of the rice protein in preschool children body, life
Price and net protein utilization are respectively 88.8%, 90.0%, 79.9%, are especially suitable for infant foods.In addition rice
Albumen also has important health care, recent studies indicate that, rice protein becomes to anti-diabetic, anti-cholesterol and anticancer
Have certain effect Deng all;As can be seen here, develop high-purity rice protein and there is good market prospect.Rice is China's yield
Maximum grain variety, rice in addition to directly eating also are used for the food such as starch sugar, cake, wine brewing as primary raw material and add deeply
Industrial and commercial bank's industry;In starch sugar industry, refined sugar using amylase, Glucoamylase hydrolysiss rice more, sugaring is generated after hydrolysis discarded
Thing Solid state, the main component of Solid state is protein, remaining starch class material and a small amount of fat and cellulose;In rice
Protein content is about 7%-8%, and the protein content in Solid state can typically reach 65%, even more high.Therefore, rice
Slag is the good raw material source for preparing rice protein and big mpd polypeptide.
During rice starch hydrolysis sugaring, as the process temperatures such as liquefaction are higher, cause protein denaturation, disulfide bond
Destruction is serious, and the dissolubility of rice protein, emulsibility, retentiveness and foaming characteristic are very poor;In addition, rice protein molecular weight compared with
Greatly, so as to the nutrition balance and added value that result in rice protein product are relatively low, seriously limit the application model of rice protein
Enclose.Therefore, dissolubility, emulsibility, retentiveness and the preferable rice polypeptide products of foaming characteristic are developed imperative.Due to the spy of enzyme
The features such as opposite sex, high efficiency, current enzyme hydrolysis method is the main method for preparing big mpd polypeptide.But existing zymotechnic generally existing
The shortcomings of equipment is complicated, reaction condition is higher, step is complicated, reaction time is long, causes the yield and content of big mpd polypeptide not high.
The content of the invention
The present invention is exactly in view of the shortcomings of the prior art, there is provided one kind prepares high-purity rice protein using Solid state
With the method for big mpd polypeptide, this method Preparation equipment and technological process it is relatively simple, reaction condition require it is relatively low, greatly improve
The yield and content of big mpd polypeptide, meets actual operation requirements.
To solve the above problems, the technical solution used in the present invention is as follows:
A kind of method that utilization Solid state prepares high-purity rice protein and big mpd polypeptide, specifically includes following steps:
Step(1), pretreatment of raw material:Its weight 3-4 times is added in Solid state produced by rice sugar manufacturing process
Then hot water, washing 3 times filtering means dehydration, the filter cake for obtaining is refined using colloid mill, is obtained by hydrocyclone desanding
To slip again water use regulation to the rice slurry that pulp density is in terms of Solid state weight 18~24%;
Step(2), enzyme digestion reaction:Using aqueous alkali by step(1)The pH value of the rice slurry for obtaining is transferred to 5.8~6.2, presses
According to 0.2~0.5% addition compound enzyme of Solid state quality, and react 1 hour at a temperature of 58~62 DEG C;
Step(3), washing filtering:By step(2)Obtain rice slurry disc centrifuge and wash separation, be washed with water wash to
Soluble solid content in eluate collects heavy phase component, as rice protein crude product below 1%;
Step(4), albumen prepare:By step(3)The rice protein crude product for obtaining carries out dehydrate after high temperature sterilize, then adopts
Subcritical abstraction equipment defat, is milled to 300~500 mesh with super grinding machine is super, is packaged to be finished product;
Step(5), polypeptide prepare:With step(4)The rice protein finished product for obtaining is raw material, and to the raw material with 1:10 feed liquid
Than the compound enzyme with egg albumen powder quality 2%(Vegetable protein compound enzyme:Food flavor enzyme=1:1)Carry out enzymolysis 24h, centrifugation, spraying
It is dried, you can obtain big mpd polypeptide finished product.
As the improvement of above-mentioned technical proposal, step(1)Described in hot water temperature be 80~100 DEG C.
As the improvement of above-mentioned technical proposal, step(1)Described in Solid state fine grinding granularity be 300~500 mesh.
As the improvement of above-mentioned technical proposal, step(2)Described in compound enzyme be amylase and cellulase.
As the improvement of above-mentioned technical proposal, step(4)Described in high temperature sterilize be temperature be 135 DEG C~150 DEG C, when
Between to carry out under conditions of 4~15 seconds.
As the improvement of above-mentioned technical proposal, step(4)Described in subcritical abstraction be 0.5~1 little in extraction time
When, extracting pressure be 0.2~0.7MPa, temperature be 40~50 DEG C under conditions of carry out.
Compared with prior art, the implementation result of the present invention is as follows for the present invention:
The method that a kind of utilization Solid state of the present invention prepares high-purity rice protein and big mpd polypeptide, with Solid state as raw material
Highly purified rice protein and big mpd polypeptide are prepared, this method Preparation equipment and technological process are relatively simple, reaction condition is required
It is relatively low so that the protein content of rice protein is higher than 85%, and fat content is less than 2%;Rice Purity is higher than 90%, and rice is more
Peptides extraction rate is higher than 70%, significantly improves the added value of Solid state, solves garbage present in rice starch sugaring process
The problems such as processing difficult, environmental pollution, suitable industrialized production.
Specific embodiment
Below in conjunction with specific embodiments illustrating present disclosure.
Specific embodiment 1
A kind of method that utilization Solid state prepares high-purity rice protein and big mpd polypeptide, specifically includes following steps:
Step(1), pretreatment of raw material:
The hot water of 3 times of its weight, washing 3 times is added simultaneously to filter de- in Solid state produced by rice sugar manufacturing process
Then water, the filter cake for obtaining is refined using colloid mill by hydrocyclone desanding, and water use regulation is arrived the slip for obtaining again
Pulp density is in terms of Solid state weight 18% rice slurry;
Step(2), enzyme digestion reaction:
Using aqueous alkali by step(1)The pH value of the rice slurry for obtaining is transferred to 5.8, adds according to the 0.2% of Solid state quality
Compound enzyme, and react 1 hour at a temperature of 58 DEG C;
Step(3), washing filtering:
By step(2)Obtain rice slurry disc centrifuge and wash separation, be washed with water the solubility washed into eluate
Solid content collects heavy phase component, as rice protein crude product below 1%;
Step(4), albumen prepare:
By step(3)The rice protein crude product for obtaining carries out dehydrate after high temperature sterilize, then de- using subcritical abstraction equipment
Fat, is milled to 300 mesh with super grinding machine is super, is packaged to be finished product;
Step(5), polypeptide prepare:
With step(4)The rice protein finished product for obtaining is raw material, and to the raw material with 1:10 solid-liquid ratio and egg albumen powder quality 2%
Compound enzyme(Vegetable protein compound enzyme:Food flavor enzyme=1:1)Enzymolysis 24h is carried out, centrifugation is spray-dried, you can obtain rice many
Peptide finished product.
Further with improvement, step(1)Described in hot water temperature be 80, step(1)In Solid state fine grinding granularity
For 300 mesh;
Further with improvement, step(2)Middle compound enzyme is amylase and cellulase.
Specifically, step(4)High temperature sterilizing be temperature be 135 DEG C, the time be 4 seconds under conditions of carry out;Step(4)
Subcritical extraction be extraction time be 0.5 hour, extracting pressure be 0.2MPa, temperature be 40 DEG C under conditions of carry out.
Wherein, the preparation technology equipment is relatively simple, reaction condition requires relatively low, and each parameter setting causes rice protein
Protein content is higher than 85%, and fat content is less than 2%;Rice Purity reaches 90%, big mpd polypeptide extraction rate reached to 70%, significantly
Improve the added value of Solid state.
Specific embodiment 2
A kind of method that utilization Solid state prepares high-purity rice protein and big mpd polypeptide, specifically includes following steps:
Step(1), pretreatment of raw material:
The hot water of 3.5 times of its weight, washing 3 times is added simultaneously to filter de- in Solid state produced by rice sugar manufacturing process
Then water, the filter cake for obtaining is refined using colloid mill by hydrocyclone desanding, and water use regulation is arrived the slip for obtaining again
Pulp density is in terms of Solid state weight 21% rice slurry;
Step(2), enzyme digestion reaction:
Using aqueous alkali by step(1)The pH value of the rice slurry for obtaining is transferred to 6, adds according to the 0.35% of Solid state quality
Compound enzyme, and react 1 hour at a temperature of 60 DEG C;
Step(3), washing filtering:
By step(2)Obtain rice slurry disc centrifuge and wash separation, be washed with water the solubility washed into eluate
Solid content collects heavy phase component, as rice protein crude product below 1%;
Step(4), albumen prepare:
By step(3)The rice protein crude product for obtaining carries out dehydrate after high temperature sterilize, then de- using subcritical abstraction equipment
Fat, is milled to 400 mesh with super grinding machine is super, is packaged to be finished product;
Step(5), polypeptide prepare:
With step(4)The rice protein finished product for obtaining is raw material, and to the raw material with 1:10 solid-liquid ratio and egg albumen powder quality 2%
Compound enzyme(Vegetable protein compound enzyme:Food flavor enzyme=1:1)Enzymolysis 24h is carried out, centrifugation is spray-dried, you can obtain rice many
Peptide finished product.
Further with improvement, step(1)In hot water temperature be 90 DEG C, step(1)In Solid state fine grinding granularity be
400 mesh.
Further with improvement, step(2)Middle compound enzyme is amylase and cellulase.
Specifically, step(4)High temperature sterilizing be temperature be 142.5 DEG C, the time be 9.5 seconds under conditions of carry out, walk
Suddenly(4)Subcritical extraction be extraction time be 0.75 hour, extracting pressure be 0.45MPa, temperature be 45 DEG C under conditions of
Carry out.
Wherein, the preparation technology equipment is relatively simple, reaction condition requires relatively low, and each parameter setting causes rice protein
Protein content is higher than 85%, and fat content is less than 2%;Rice Purity is higher than 90%, and big mpd polypeptide extraction ratio is higher than 70%, significantly
The added value of Solid state is improve, offal treatment hardly possible, environmental pollution etc. present in rice starch sugaring process is solved and is asked
Topic, suitable industrialized production.
Specific embodiment 3
A kind of method that utilization Solid state prepares high-purity rice protein and big mpd polypeptide, specifically includes following steps:
Step(1), pretreatment of raw material:
The hot water of 4 times of its weight, washing 3 times is added simultaneously to filter de- in Solid state produced by rice sugar manufacturing process
Then water, the filter cake for obtaining is refined using colloid mill by hydrocyclone desanding, and water use regulation is arrived the slip for obtaining again
Pulp density is in terms of Solid state weight 24% rice slurry;
Step(2), enzyme digestion reaction:
Using aqueous alkali by step(1)The pH value of the rice slurry for obtaining is transferred to 6.2, adds according to the 0.5% of Solid state quality
Compound enzyme, and react 1 hour at a temperature of 62 DEG C;
Step(3), washing filtering:
By step(2)Obtain rice slurry disc centrifuge and wash separation, be washed with water the solubility washed into eluate
Solid content collects heavy phase component, as rice protein crude product below 1%;
Step(4), albumen prepare:
By step(3)The rice protein crude product for obtaining carries out dehydrate after high temperature sterilize, then de- using subcritical abstraction equipment
Fat, is milled to 500 mesh with super grinding machine is super, is packaged to be finished product;
Step(5), polypeptide prepare:
With step(4)The rice protein finished product for obtaining is raw material, and to the raw material with 1:10 solid-liquid ratio and egg albumen powder quality 2%
Compound enzyme(Vegetable protein compound enzyme:Food flavor enzyme=1:1)Enzymolysis 24h is carried out, centrifugation is spray-dried, you can obtain rice many
Peptide finished product.
Further with improvement, step(1)In hot water temperature be 100 DEG C, step(1)In Solid state fine grinding granularity be
500 mesh.
Further with improvement, step(2)Middle compound enzyme is amylase and cellulase.
Specifically, step(4)High temperature sterilizing be temperature be 150 DEG C, the time be 15 seconds under conditions of carry out, step
(4)Subcritical extraction be extraction time be 1 hour, extracting pressure be 0.7MPa, temperature be 50 DEG C under conditions of carry out.
Additionally, this method Preparation equipment and technological process are relatively simple, reaction condition requires relatively low so that rice protein
Protein content reaches 85%, and fat content is less than 2%;Rice Purity is higher than 90%, and big mpd polypeptide extraction ratio is higher than 70%, significantly
Improve the added value of Solid state, suitable industrialized production.
Note:What the design parameter span in each processing step of the present invention can be reached according to required by actual preparation
The standard of protein content, fat content, rice Purity and big mpd polypeptide extraction ratio is setting.
Above content is detailed description made for the present invention in conjunction with specific embodiments, it is impossible to assert that the present invention is concrete real
Apply and be only limitted to these explanations.For those skilled in the art, before without departing from present inventive concept
Put, some simple deduction or replace can also be made, should all be considered as belonging to the scope of protection of the invention.
Claims (6)
1. a kind of method that utilization Solid state prepares high-purity rice protein and big mpd polypeptide, it is characterised in that:Specifically include with
Lower step:
Step(1), pretreatment of raw material:Its weight 3-4 times is added in Solid state produced by rice sugar manufacturing process
Then hot water, washing 3 times filtering means dehydration, the filter cake for obtaining is refined using colloid mill, is obtained by hydrocyclone desanding
To slip again water use regulation to the rice slurry that pulp density is in terms of Solid state weight 18~24%;
Step(2), enzyme digestion reaction:Using aqueous alkali by step(1)The pH value of the rice slurry for obtaining is transferred to 5.8~6.2, presses
According to 0.2~0.5% addition compound enzyme of Solid state quality, and react 1 hour at a temperature of 58~62 DEG C;
Step(3), washing filtering:By step(2)Obtain rice slurry disc centrifuge and wash separation, be washed with water wash to
Soluble solid content in eluate collects heavy phase component, as rice protein crude product below 1%;
Step(4), albumen prepare:By step(3)The rice protein crude product for obtaining carries out dehydrate after high temperature sterilize, then adopts
Subcritical abstraction equipment defat, is milled to 300~500 mesh with super grinding machine is super, is packaged to be finished product;
Step(5), polypeptide prepare:With step(4)The rice protein finished product for obtaining is raw material, and to the raw material with 1:10 feed liquid
Than the compound enzyme with egg albumen powder quality 2%(Vegetable protein compound enzyme:Food flavor enzyme=1:1)Carry out enzymolysis 24h, centrifugation, spraying
It is dried, you can obtain big mpd polypeptide finished product.
2. a kind of method that utilization Solid state prepares high-purity rice protein and big mpd polypeptide according to claim 1, which is special
Levy and be:Step(1)Described in hot water temperature be 80~100 DEG C.
3. a kind of method that utilization Solid state prepares high-purity rice protein and big mpd polypeptide according to claim 1, which is special
Levy and be:Step(1)Described in Solid state fine grinding granularity be 300~500 mesh.
4. a kind of method that utilization Solid state prepares high-purity rice protein and big mpd polypeptide according to claim 1, which is special
Levy and be:Step(2)Described in compound enzyme be amylase and cellulase.
5. a kind of method that utilization Solid state prepares high-purity rice protein and big mpd polypeptide according to claim 1, which is special
Levy and be:Step(4)Described in high temperature sterilize be temperature be 135 DEG C~150 DEG C, the time be 4~15 seconds under conditions of carry out.
6. a kind of method that utilization Solid state prepares high-purity rice protein and big mpd polypeptide according to claim 1, which is special
Levy and be:Step(4)Described in subcritical abstraction be extraction time be 0.5~1 hour, extracting pressure be 0.2~0.7MPa,
Temperature be 40~50 DEG C under conditions of carry out.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110731409A (en) * | 2019-10-09 | 2020-01-31 | 安徽金太阳食品有限公司 | method for processing high protein rice flour with broken rice |
CN111955591A (en) * | 2020-08-28 | 2020-11-20 | 天津黄庄稻香米业有限公司 | Method for preparing antioxidant rice protein by using rice residues |
CN115777829A (en) * | 2022-11-25 | 2023-03-14 | 滁州金弘安米业有限公司 | Method for preparing high-purity rice protein and rice polypeptide by using rice |
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CN1896261A (en) * | 2006-06-15 | 2007-01-17 | 普洱永吉生物技术有限责任公司 | Production of rice-starch and rice-protein polypeptide powder by composite enzyme method |
CN102150737A (en) * | 2011-04-12 | 2011-08-17 | 江南大学 | Method for preparing high-purity rice protein and acquired products by using same |
CN104418944A (en) * | 2013-09-10 | 2015-03-18 | 郑州市中食农产品加工研究院 | Technology for separating multiple bioactive components in maize germ |
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Patent Citations (4)
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WO2005096804A2 (en) * | 2004-03-08 | 2005-10-20 | Syngenta Participations Ag | Self-processing plants and plant parts |
CN1896261A (en) * | 2006-06-15 | 2007-01-17 | 普洱永吉生物技术有限责任公司 | Production of rice-starch and rice-protein polypeptide powder by composite enzyme method |
CN102150737A (en) * | 2011-04-12 | 2011-08-17 | 江南大学 | Method for preparing high-purity rice protein and acquired products by using same |
CN104418944A (en) * | 2013-09-10 | 2015-03-18 | 郑州市中食农产品加工研究院 | Technology for separating multiple bioactive components in maize germ |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN110731409A (en) * | 2019-10-09 | 2020-01-31 | 安徽金太阳食品有限公司 | method for processing high protein rice flour with broken rice |
CN111955591A (en) * | 2020-08-28 | 2020-11-20 | 天津黄庄稻香米业有限公司 | Method for preparing antioxidant rice protein by using rice residues |
CN115777829A (en) * | 2022-11-25 | 2023-03-14 | 滁州金弘安米业有限公司 | Method for preparing high-purity rice protein and rice polypeptide by using rice |
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Application publication date: 20170419 |