CN106986917A - One kind method of separating protein from tea oil oil reservoir - Google Patents
One kind method of separating protein from tea oil oil reservoir Download PDFInfo
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- CN106986917A CN106986917A CN201710237595.6A CN201710237595A CN106986917A CN 106986917 A CN106986917 A CN 106986917A CN 201710237595 A CN201710237595 A CN 201710237595A CN 106986917 A CN106986917 A CN 106986917A
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- oil reservoir
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- Proteomics, Peptides & Aminoacids (AREA)
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Abstract
The present invention relates to one kind from tea oil oil reservoir method of separating protein, methods described is as follows：S1：Camellia seed kernel powder is dried after soaked in absolute ethyl alcohol, the stirring that adds water, standing are positioned to oil reservoir and water layer, are then centrifuged for separating to obtain oil reservoir；S2：Oil reservoir obtained by S1 is added water to wash and obtains the oil reservoir containing protein；S3：Phosphatidase is added into oil reservoir obtained by S2, then adds water and produces protein in being centrifuged after 2~4h of enzymolysis stirring under the conditions of 45~50 DEG C；Wherein, the consumption of the phosphatidase is 0.1~the 1 ‰ of reservoir volume.The Separation of Proteins that the method that the present invention is provided will can be scattered in tea oil oil reservoir, can realize the recycling of protein；And the technique of the present invention is simple, is easy to industrial applications.
Description
Technical field
The present invention relates to biological technical field, and in particular to one kind method of separating protein from tea oil oil reservoir.
Background technology
Current vegetable fat produce it is main use lixiviation process and mechanical expression method, but these methods security, economy,
The problem of environmentally friendly aspect all has certain.Water substitution extraction edible oil is environment-friendly, and foodsafety is good, and organic solvent-free is residual
Stay, will not produce the harmful substances such as BaP, trans-fatty acid, the color with oil color of extraction is shallow, acid value is low, peroxide value is low, raw
Manage activity substance content high, can be used as edible oil without refining, and can obtain high-quality while high-quality grease is produced
The products such as the protein and fecula of amount.Therefore, water substitution, which is just gradually substituting lixiviation process and mechanical expression method, turns into main flow
Grease extraction.
Water substitution extract grease often produce boiled oil layer, emulsus oil and water layer, but camellia seed kernel powder using water substitution extract when,
The tea oil extracted after being dried again through ethanol immersion has had to oil reservoir and water layer, and emulsus oil is not produced；And gained oil reservoir
It is opaque, dispersed protein in oil reservoir.Prior art not yet has open source literature report how from tea oil oil reservoir
Method of separating protein.
Therefore, still need to study a kind of efficiency high, the technique method of separating protein simply from tea oil oil reservoir.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of side of protein isolate matter in oil reservoir from tea oil
Method, the Separation of Proteins that the method that the present invention is provided will can be scattered in tea oil oil reservoir, can realize the recycling of protein；And
And the technique of the present invention is simple, is easy to industrial applications.
To achieve the above object, the present invention is adopted the following technical scheme that：
One kind method of separating protein from tea oil oil reservoir, methods described is as follows：
S1：Camellia seed kernel powder is dried after soaked in absolute ethyl alcohol, the stirring that adds water, standing are positioned to oil reservoir and water layer, Ran Houli
The heart separates to obtain oil reservoir；
S2：Oil reservoir obtained by S1 is added water to wash and obtains the oil reservoir containing protein；
S3：Phosphatidase is added into oil reservoir obtained by S2, then adds water and is centrifuged in after 2~4h of enzymolysis stirring under the conditions of 45~50 DEG C
Separation produces protein；
Wherein, the consumption of the phosphatidase is 0.1~the 1 ‰ of reservoir volume.
Preferably, the consumption of phosphatidase described in S3 is 0.5~the 1 ‰ of reservoir volume.
Preferably, the hydrolysis temperature in S3 is 45 DEG C, and enzymolysis time is 3h.
Preferably, the mass ratio of camellia seed kernel powder described in S1 and water is 1:5.
Preferably, the volume ratio of oil reservoir and water is 3 in S2:1.
Preferably, the number of times washed described in S2 is 2~3 times.
Preferably, the enzyme activity of phosphatidase described in S3 is 10KU/g.
Compared with prior art, the present invention has the advantages that：
The Separation of Proteins that the method that the present invention is provided will can be scattered in tea oil oil reservoir, can realize the recycling of protein；
And the technique of the present invention is simple, is easy to industrial applications.
Brief description of the drawings
The process chart for the method that Fig. 1 provides for the present invention.
Embodiment
With reference to example, the present invention is described further, and the given examples are served only to explain the present invention, but the present invention
Claimed scope is not limited to the scope that example is stated.
Embodiment 1
One kind method of separating protein from tea oil oil reservoir, methods described is as follows：
S1：Camellia seed kernel powder is dried after soaked in absolute ethyl alcohol, the stirring that adds water, standing are positioned to oil reservoir and water layer, Ran Houli
The heart separates to obtain oil reservoir；
S2：Oil reservoir obtained by S1 is added water to wash and obtains the oil reservoir containing protein；
S3：Phosphatidase is added into oil reservoir obtained by S2, then adds water and is produced in being centrifuged after enzymolysis stirring 2h under the conditions of 45
Protein；
In the present embodiment, the consumption of the phosphatidase is the 0.1 ‰ of reservoir volume.
Tea oil oil reservoir after the method separation that the present embodiment is provided is as clear as crystal, and this shows that this method can be complete by protein
Separated entirely from tea oil oil reservoir.
Embodiment 2
One kind method of separating protein from tea oil oil reservoir, methods described is as follows：
S1：Camellia seed kernel powder is dried after soaked in absolute ethyl alcohol, the stirring that adds water, standing are positioned to oil reservoir and water layer, Ran Houli
The heart separates to obtain oil reservoir；
S2：Oil reservoir obtained by S1 is added water to wash and obtains the oil reservoir containing protein；
S3：Phosphatidase is added into oil reservoir obtained by S2, then adds water and is centrifuged i.e. in after enzymolysis stirring 4h under the conditions of 50 DEG C
Obtain protein；
In the present embodiment, the consumption of the phosphatidase is the 0.2 ‰ of reservoir volume；The matter of camellia seed kernel powder described in S1 and water
Amount is than being 1:5；The volume ratio of oil reservoir and water is 3 in S2:1, the number of times of washing is 2~3 times；The enzyme activity of phosphatidase described in S3 is
10KU/g。
Tea oil oil reservoir after the method separation that the present embodiment is provided is as clear as crystal, and this shows that this method can be complete by protein
Separated entirely from tea oil oil reservoir.
Embodiment 3
One kind method of separating protein from tea oil oil reservoir, methods described is as follows：
S1：Camellia seed kernel powder is dried after soaked in absolute ethyl alcohol, the stirring that adds water, standing are positioned to oil reservoir and water layer, Ran Houli
The heart separates to obtain oil reservoir；
S2：Oil reservoir obtained by S1 is added water to wash and obtains the oil reservoir containing protein；
S3：Phosphatidase is added into oil reservoir obtained by S2, then adds water and is centrifuged i.e. in after enzymolysis stirring 3h under the conditions of 47 DEG C
Obtain protein；
In the present embodiment, the consumption of the phosphatidase is the 0.5 ‰ of reservoir volume；The matter of camellia seed kernel powder described in S1 and water
Amount is than being 1:5；The volume ratio of oil reservoir and water is 3 in S2:1, the number of times of washing is 2~3 times；The enzyme activity of phosphatidase described in S3 is
10KU/g。
Tea oil oil reservoir after the method separation that the present embodiment is provided is as clear as crystal, and this shows that this method can be complete by protein
Separated entirely from tea oil oil reservoir.
Embodiment 4
One kind method of separating protein from tea oil oil reservoir, methods described is as follows：
S1：Camellia seed kernel powder is dried after soaked in absolute ethyl alcohol, the stirring that adds water, standing are positioned to oil reservoir and water layer, Ran Houli
The heart separates to obtain oil reservoir；
S2：Oil reservoir obtained by S1 is added water to wash and obtains the oil reservoir containing protein；
S3：Phosphatidase is added into oil reservoir obtained by S2, then adds water and is centrifuged in after enzymolysis stirring 2.5h under the conditions of 48 DEG C
Produce protein；
In the present embodiment, the consumption of the phosphatidase is the 1 ‰ of reservoir volume；The quality of camellia seed kernel powder described in S1 and water
Than for 1:5；The volume ratio of oil reservoir and water is 10 in S2:1, the number of times of washing is 2~3 times；The enzyme activity of phosphatidase described in S3 is
10KU/g。
Tea oil oil reservoir after the method separation that the present embodiment is provided is as clear as crystal, and this shows that this method can be complete by protein
Separated entirely from tea oil oil reservoir.
Claims (9)
1. one kind method of separating protein from tea oil oil reservoir, it is characterised in that methods described is as follows：
S1：Camellia seed kernel powder is dried after soaked in absolute ethyl alcohol, the stirring that adds water, standing are positioned to oil reservoir and water layer, Ran Houli
The heart separates to obtain oil reservoir；
S2：Oil reservoir obtained by S1 is added water to wash and obtains the oil reservoir containing protein；
S3：Phosphatidase is added into oil reservoir obtained by S2, then adds water and is centrifuged in after 2~4h of enzymolysis stirring under the conditions of 45~50 DEG C
Separation produces protein；
Wherein, the consumption of the phosphatidase is 0.1~the 1 ‰ of reservoir volume.
2. the method for separating protein from tea oil oil reservoir according to claim 1, it is characterised in that phosphatidase described in S3
Consumption be reservoir volume 0.5~1 ‰.
3. the method for separating protein from tea oil oil reservoir according to claim 1, it is characterised in that the hydrolysis temperature in S3
For 45 DEG C, enzymolysis time is 3h.
4. the method for separating protein from tea oil oil reservoir according to claim 1, it is characterised in that tea seed described in S1
The mass ratio of benevolence powder and water is 1：3~20.
5. the method for separating protein from tea oil oil reservoir according to claim 4, it is characterised in that tea seed described in S1
The mass ratio of benevolence powder and water is 1:5.
6. the method for separating protein from tea oil oil reservoir according to claim 1, it is characterised in that oil reservoir and water in S2
Volume ratio is 1~10：1.
7. the method for separating protein from tea oil oil reservoir according to claim 6, it is characterised in that oil reservoir and water in S2
Volume ratio is 10:1.
8. the method for separating protein from tea oil oil reservoir according to claim 1, it is characterised in that washed described in S2
Number of times is 2~3 times.
9. the method for separating protein from tea oil oil reservoir according to claim 1, it is characterised in that phosphatidase described in S3
Enzyme activity be 10KU/g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201710237595.6A CN106986917A (en) | 2017-04-12 | 2017-04-12 | One kind method of separating protein from tea oil oil reservoir |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710237595.6A CN106986917A (en) | 2017-04-12 | 2017-04-12 | One kind method of separating protein from tea oil oil reservoir |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102766533A (en) * | 2012-08-21 | 2012-11-07 | 东北农业大学 | Biological enzyme demulsification method for extracting soybean oil and fat by aqueous enzymatic method |
| CN103820215A (en) * | 2014-03-12 | 2014-05-28 | 韶关学院 | Oil processing method |
| WO2015150372A1 (en) * | 2014-04-01 | 2015-10-08 | Dupont Nutrition Biosciences Aps | Method for increasing crude palm oil yields |
| CN105349246A (en) * | 2015-11-12 | 2016-02-24 | 东北农业大学 | Method for synchronous extraction of grease and protein peptide from shiny-leaved yellowhorn |
-
2017
- 2017-04-12 CN CN201710237595.6A patent/CN106986917A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102766533A (en) * | 2012-08-21 | 2012-11-07 | 东北农业大学 | Biological enzyme demulsification method for extracting soybean oil and fat by aqueous enzymatic method |
| CN103820215A (en) * | 2014-03-12 | 2014-05-28 | 韶关学院 | Oil processing method |
| WO2015150372A1 (en) * | 2014-04-01 | 2015-10-08 | Dupont Nutrition Biosciences Aps | Method for increasing crude palm oil yields |
| CN105349246A (en) * | 2015-11-12 | 2016-02-24 | 东北农业大学 | Method for synchronous extraction of grease and protein peptide from shiny-leaved yellowhorn |
Non-Patent Citations (1)
| Title |
|---|
| SOLMAZ TABTABAEI等: "Aqueous and enzymatic extraction processes for the production of food-grade proteins and industrial oil from dehulled yellow mustard flour", 《FOOD RESEARCH INTERNATIONAL》 * |
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