CN105767455A - Method for preparing hickory nut protein by enzyme method - Google Patents
Method for preparing hickory nut protein by enzyme method Download PDFInfo
- Publication number
- CN105767455A CN105767455A CN201610152003.6A CN201610152003A CN105767455A CN 105767455 A CN105767455 A CN 105767455A CN 201610152003 A CN201610152003 A CN 201610152003A CN 105767455 A CN105767455 A CN 105767455A
- Authority
- CN
- China
- Prior art keywords
- enzyme
- enzymolysis
- alkali
- liquid
- albumen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 124
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 124
- 238000000034 method Methods 0.000 title claims abstract description 80
- 241000899834 Obovaria olivaria Species 0.000 title claims abstract description 39
- 108010003571 Nut Proteins Proteins 0.000 title abstract 5
- 239000007788 liquid Substances 0.000 claims abstract description 82
- 239000003513 alkali Substances 0.000 claims abstract description 74
- 239000000843 powder Substances 0.000 claims abstract description 50
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 46
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 46
- 239000002994 raw material Substances 0.000 claims abstract description 32
- 238000001556 precipitation Methods 0.000 claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 238000002156 mixing Methods 0.000 claims abstract description 17
- 238000000605 extraction Methods 0.000 claims abstract description 15
- 238000002360 preparation method Methods 0.000 claims abstract description 6
- 238000004108 freeze drying Methods 0.000 claims abstract description 4
- 229940088598 enzyme Drugs 0.000 claims description 119
- 210000000582 semen Anatomy 0.000 claims description 59
- 238000001976 enzyme digestion Methods 0.000 claims description 42
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 36
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 36
- 238000006243 chemical reaction Methods 0.000 claims description 28
- 229940059442 hemicellulase Drugs 0.000 claims description 28
- 108010002430 hemicellulase Proteins 0.000 claims description 28
- 108020004410 pectinesterase Proteins 0.000 claims description 28
- 239000002253 acid Substances 0.000 claims description 20
- 239000000284 extract Substances 0.000 claims description 14
- 230000003301 hydrolyzing effect Effects 0.000 claims description 14
- 108010070456 protopectinase Proteins 0.000 claims description 14
- 230000035484 reaction time Effects 0.000 claims description 14
- 229930000044 secondary metabolite Natural products 0.000 claims description 14
- 241000209094 Oryza Species 0.000 claims description 12
- 235000007164 Oryza sativa Nutrition 0.000 claims description 12
- 235000009566 rice Nutrition 0.000 claims description 12
- 238000010792 warming Methods 0.000 claims description 12
- 230000010355 oscillation Effects 0.000 claims description 10
- 238000012792 lyophilization process Methods 0.000 claims description 6
- 238000002791 soaking Methods 0.000 claims description 6
- 239000002904 solvent Substances 0.000 claims description 6
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 230000002255 enzymatic effect Effects 0.000 abstract 6
- 239000000413 hydrolysate Substances 0.000 abstract 6
- 239000002002 slurry Substances 0.000 abstract 4
- 238000003916 acid precipitation Methods 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 34
- 241000723418 Carya Species 0.000 description 3
- 235000001014 amino acid Nutrition 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 239000003921 oil Substances 0.000 description 3
- 235000019198 oils Nutrition 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 238000004904 shortening Methods 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 241000758789 Juglans Species 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108010064851 Plant Proteins Proteins 0.000 description 1
- 235000019498 Walnut oil Nutrition 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 235000021118 plant-derived protein Nutrition 0.000 description 1
- 239000008170 walnut oil Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method for preparing hickory nut protein by an enzyme method. The method comprises the following steps of uniformly mixing hickory nut powder raw materials with water so as to obtain primary slurry, adjusting the pH of the primary slurry, and adding primary complex enzymes for enzymolysis so as to obtain primary enzymatic hydrolysate; performing microwave treatment on the primary enzymatic hydrolysate so as to obtain microwave enzymatic hydrolysate; performing primary alkali extraction on the microwave enzymatic hydrolysate so as to obtain primary protein liquid and residue precipitation; uniformly mixing the residue precipitation with water so as to obtain secondary slurry; adjusting the pH of the secondary slurry, adding secondary complex enzymes for enzymolysis, and performing ultrasonic vibration treatment in the process of the enzymolysis so as to obtain secondary enzymatic hydrolysate; performing secondary alkali extraction on the secondary enzymatic hydrolysate so as to obtain secondary protein liquid; uniformly mixing the primary protein liquid and the secondary protein liquid, adjusting pH, and performing acid precipitation treatment so as to obtain protein powder; and performing freeze drying treatment on the protein powder so as to obtain the hickory nut protein. According to the method for preparing the hickory nut protein by the enzyme method, provided by the invention, the technology is safe, the preparation is convenient, and the extraction rate of the hickory nut protein is high.
Description
Technical field
The present invention relates to Semen Caryae Cathayensis protein techniques field, be specifically related to a kind of method that enzyme process prepares Semen Caryae Cathayensis albumen.
Background technology
Semen Juglandis has another name called Semen Juglandis, Qiang's Fructus Persicae, belongs to Juglandaceae walnut, is a kind of nutritive value and all significantly high precious fruit tree of economic worth；Walnut oil, Semen Juglandis contains the protein of 18%～24%, the carbohydrate of 12%～16%, the cellulose of 1.5%～2.0%, the mineral of 1.7%～2.0%, it can be seen that, the processing of Semen Caryae Cathayensis should mainly based on hickory oil and Semen Caryae Cathayensis albumen.Semen Caryae Cathayensis albumen is as a kind of new plant protein resource, its titer is close with animal egg hundred, amino acid classes is complete, wherein comprise multiple essential amino acid, especially arginine, glutamic acid, histidine, TYR equal size are high, 8 seed amino acid content ratio are reasonable, close to the standard amino acid proportioning that FAO (Food and Agriculture Organization of the United Nation) and World Health Organization (WHO) specify, have significantly high edibility and health care.Therefore, it is necessary to study a kind of method preparing Semen Caryae Cathayensis albumen.
Summary of the invention
For the weak point existed in above-mentioned technology, the invention provides a kind of process safety, prepare the method that enzyme process convenient, that Semen Caryae Cathayensis protein extracting ratio is high prepares Semen Caryae Cathayensis albumen.
The technical solution adopted for the present invention to solve the technical problems is: a kind of enzyme process prepares the method for Semen Caryae Cathayensis albumen, comprises the steps: the preparation of step one, hickory nut powder raw material；Step 2, primary enzymolysis: hickory nut powder raw material and water are mixed and prepares a serosity, regulate a serosity pH, and add once-combined enzyme enzymolysis, treat that enzyme digestion reaction terminates to carry out enzyme denaturing process, obtain primary enzymolysis liquid, and primary enzymolysis liquid is carried out microwave treatment, obtain microwave enzymolysis solution；Step 3, once extraction: microwave enzymolysis solution carries out an alkali and carries, obtain an alkali extract, and be centrifugally separating to obtain a protein liquid and residue precipitation；Step 4, secondary enzymolysis: residue precipitation and water mixing are prepared secondary serosity, regulates secondary serosity pH, add secondary complex enzyme zymohydrolysis, and in enzymolysis process, carry out sonic oscillation process, treat that enzyme digestion reaction terminates to carry out enzyme denaturing process, obtain secondary enzymolysis liquid；Step 5, second extraction: secondary enzymolysis liquid is carried out secondary alkali and carries, obtain secondary alkali extract, and be centrifugally separating to obtain secondary protein liquid；Step 6, acid are sunk: a protein liquid and secondary protein liquid are mixed, and carry out the heavy process of acid, be centrifugally separating to obtain egg albumen powder after regulating pH；Step 7, lyophilization: egg albumen powder is carried out lyophilization process, obtain Semen Caryae Cathayensis albumen.
Preferably, in described step one, the preparation process of hickory nut powder raw material is: adopt No. six solvent soakings after being shelled by Semen Caryae Cathayensis, the Semen Caryae Cathayensis dregs of rice of defat are obtained after precipitation, its resid amount is 1.4～2.0%, and is pulverized and sieved by the Semen Caryae Cathayensis dregs of rice of defat and obtain the hickory nut powder raw material that granular size is 100～150 mesh sieves.
Preferably, in described step 2, the process of primary enzymolysis is as follows: hickory nut powder raw material and water are pressed solid-liquid ratio 1g:15～25mL mixing and prepares a serosity, a serosity adds hydrochloric acid or a serosity pH is adjusted to 3～5 by citric acid, and add once-combined enzyme enzymolysis, enzyme digestion reaction temperature is 50～60 DEG C, the enzyme digestion reaction time is 4～8 hours, treat that enzyme digestion reaction terminates, it is warming up to 80～90 DEG C of enzyme denaturing 10～20 minutes, obtain primary enzymolysis liquid, and primary enzymolysis liquid is carried out microwave treatment, microwave power is 250～500W, the microwave time is 15～25 minutes, obtain microwave enzymolysis solution.
Preferably, once-combined enzyme in described step 2 adopts hemicellulase+protopectinase+pectinesterase hydrolytic enzyme+pectinesterase, the consumption of described hemicellulase, protopectinase, pectinesterase hydrolytic enzyme and pectinesterase is 1:3～6:2～4:4～8, and 1000～2000u/g that the enzyme concentration of once-combined enzyme is hickory nut powder raw material dosage.
Preferably, the condition once extracted in described step 3 is: alkali carries solid-liquid ratio 1g:15～25mL, and alkali carries pH9～12, and alkali temperature raising degree 45～55 DEG C, alkali carries time 2～4h.
Preferably, in described step 4, the process of secondary enzymolysis is as follows: residue precipitation and water are pressed 1g:4～8mL mixing and prepares secondary serosity, secondary serosity adds hydrochloric acid or secondary serosity pH is adjusted to 4～5 by citric acid, add secondary complex enzyme zymohydrolysis, enzyme digestion reaction temperature is 45～55 DEG C, the enzyme digestion reaction time is 2～5 hours, and in enzymolysis process, carry out sonic oscillation process, supersonic frequency is 2KHz, ultrasonic power is 400～800W, treat that enzyme digestion reaction terminates, be warming up to 80～90 DEG C of enzyme denaturing 10～20 minutes, obtain secondary enzymolysis liquid.
Preferably, the secondary compound enzyme in described step 4 adopts hemicellulase+saccharifying enzyme, and the consumption of described hemicellulase and saccharifying enzyme is 1:2～4, and 2000～3000u/g that the enzyme concentration of secondary compound enzyme is hickory nut powder raw material dosage.
Preferably, in described step 5, the condition of second extraction is: alkali carries solid-liquid ratio 1g:5～10mL, and alkali carries pH9～12, and alkali temperature raising degree 45～55 DEG C, alkali carries time 1～3h.
Preferably, in described step 6, the heavy process of acid is: a protein liquid and secondary protein liquid are mixed, and it is heavy that addition hydrochloric acid or Fructus Citri Limoniae acid for adjusting pH carry out acid to 4.5, and the heavy temperature of acid is 20～30 DEG C, is centrifugally separating to obtain egg albumen powder after floccule to appear.
Compared with prior art, it provides the benefit that the present invention:
(1) by adopting the once-combined enzyme being made up of hemicellulase+protopectinase+pectinesterase hydrolytic enzyme+pectinesterase to carry out primary enzymolysis, and adopt the secondary compound enzyme being made up of hemicellulase+saccharifying enzyme to carry out secondary enzymolysis, can effectively destroy the cell wall of hickory nut powder cell, intracellular albumen is made to be more easy to extraction, compared to using single enzyme to carry out enzymolysis, the extraction ratio of Semen Caryae Cathayensis albumen can be effectively improved.
(2) by primary enzymolysis liquid being carried out microwave treatment, and in secondary enzymolysis process, carrying out sonic oscillation process simultaneously, to provide suitable microenvironment to carry out solubilising protein, greatly shortening the enzyme digestion reaction time, adding the purity of Semen Caryae Cathayensis albumen.
(3) method that this enzyme process prepares hickory oil, process safety, prepare convenient, Semen Caryae Cathayensis protein extracting ratio up to more than 70.0%, extract Semen Caryae Cathayensis purity of protein up to more than 90.0%.
Accompanying drawing explanation
Fig. 1 is the process chart that enzyme process of the present invention prepares the method for Semen Caryae Cathayensis albumen.
Detailed description of the invention
Below in conjunction with accompanying drawing, the present invention is described in further detail, to make those skilled in the art can implement according to this with reference to description word.
As it is shown in figure 1, the invention provides a kind of method that enzyme process prepares Semen Caryae Cathayensis albumen, comprise the steps:
Step one, hickory nut powder raw material preparation: after Semen Caryae Cathayensis is shelled adopt No. six solvent soakings, the Semen Caryae Cathayensis dregs of rice of defat are obtained after precipitation, its resid amount is 1.4～2.0%, and is pulverized and sieved by the Semen Caryae Cathayensis dregs of rice of defat and obtain the hickory nut powder raw material that granular size is 100～150 mesh sieves.
Step 2, primary enzymolysis: hickory nut powder raw material and water are pressed solid-liquid ratio 1g:15～25mL mixing and prepares a serosity, a serosity adds hydrochloric acid or a serosity pH is adjusted to 3～5 by citric acid, and add once-combined enzyme enzymolysis, enzyme digestion reaction temperature is 50～60 DEG C, the enzyme digestion reaction time is 4～8 hours, treat that enzyme digestion reaction terminates, it is warming up to 80～90 DEG C of enzyme denaturing 10～20 minutes, obtain primary enzymolysis liquid, and primary enzymolysis liquid is carried out microwave treatment, microwave power is 250～500W, and the microwave time is 15～25 minutes, obtains microwave enzymolysis solution；Wherein, described once-combined enzyme adopts hemicellulase+protopectinase+pectinesterase hydrolytic enzyme+pectinesterase, the consumption of described hemicellulase, protopectinase, pectinesterase hydrolytic enzyme and pectinesterase is 1:3～6:2～4:4～8, and 1000～2000u/g that the enzyme concentration of once-combined enzyme is hickory nut powder raw material dosage.
Step 3, once extraction: microwave enzymolysis solution carrying out an alkali and carries, alkali carries solid-liquid ratio 1g:15～25mL, and alkali carries pH9～12, alkali temperature raising degree 45～55 DEG C, alkali carries time 2～4h, obtains an alkali extract, and is centrifugally separating to obtain a protein liquid and residue precipitation.
Step 4, secondary enzymolysis: residue precipitation and water are pressed 1g:4～8mL mixing and prepares secondary serosity, secondary serosity adds hydrochloric acid or secondary serosity pH is adjusted to 4～5 by citric acid, adding secondary complex enzyme zymohydrolysis, enzyme digestion reaction temperature is 45～55 DEG C, and the enzyme digestion reaction time is 2～5 hours, and in enzymolysis process, carry out sonic oscillation process, supersonic frequency is 2KHz, and ultrasonic power is 400～800W, treats that enzyme digestion reaction terminates, it is warming up to 80～90 DEG C of enzyme denaturing 10～20 minutes, obtains secondary enzymolysis liquid；Wherein, described secondary compound enzyme adopts hemicellulase+saccharifying enzyme, and the consumption of described hemicellulase and saccharifying enzyme is 1:2～4, and 2000～3000u/g that the enzyme concentration of secondary compound enzyme is hickory nut powder raw material dosage.
Step 5, second extraction: secondary enzymolysis liquid is carried out secondary alkali and carries, alkali carries solid-liquid ratio 1g:5～10mL, and alkali carries pH9～12, alkali temperature raising degree 45～55 DEG C, and alkali carries time 1～3h, obtains secondary alkali extract, and is centrifugally separating to obtain secondary protein liquid.
Step 6, acid are sunk: a protein liquid and secondary protein liquid are mixed, and it is heavy that addition hydrochloric acid or Fructus Citri Limoniae acid for adjusting pH carry out acid to 4.5, and sour heavy temperature is 20～30 DEG C, is centrifugally separating to obtain egg albumen powder after floccule to appear.
Step 7, lyophilization: egg albumen powder is carried out lyophilization process, obtain Semen Caryae Cathayensis albumen.
The present invention is compared with prior art, it provides the benefit that: by adopting the once-combined enzyme being made up of hemicellulase+protopectinase+pectinesterase hydrolytic enzyme+pectinesterase to carry out primary enzymolysis, and adopt the secondary compound enzyme being made up of hemicellulase+saccharifying enzyme to carry out secondary enzymolysis, can effectively destroy the cell wall of hickory nut powder cell, intracellular albumen is made to be more easy to extraction, compared to using single enzyme to carry out enzymolysis, the extraction ratio of Semen Caryae Cathayensis albumen can be effectively improved；By primary enzymolysis liquid being carried out microwave treatment, and in secondary enzymolysis process, carrying out sonic oscillation process simultaneously, to provide suitable microenvironment to carry out solubilising protein, greatly shortening the enzyme digestion reaction time, adding the purity of Semen Caryae Cathayensis albumen；This enzyme process prepares the method for hickory oil, process safety, prepare convenient, Semen Caryae Cathayensis protein extracting ratio up to more than 70.0%, the Semen Caryae Cathayensis purity of protein that extracts is up to more than 90.0%.
Embodiment 1:
Adopting No. six solvent soakings after being shelled by Semen Caryae Cathayensis, obtain the Semen Caryae Cathayensis dregs of rice of defat after precipitation, its resid amount is 1.4%, and is pulverized and sieved by the Semen Caryae Cathayensis dregs of rice of defat and obtain the hickory nut powder raw material that granular size is 125 mesh sieves；Hickory nut powder raw material and water are pressed solid-liquid ratio 1g:25mL mixing and prepares a serosity, adding hydrochloric acid in a serosity or a serosity pH is adjusted to 5 by citric acid, and add once-combined enzyme enzymolysis, enzyme digestion reaction temperature is 50 DEG C, the enzyme digestion reaction time is 6 hours, treat that enzyme digestion reaction terminates, be warming up to 90 DEG C of enzyme denaturing 10 minutes, obtain primary enzymolysis liquid, and primary enzymolysis liquid is carried out microwave treatment, microwave power is 375W, and the microwave time is 20 minutes, obtains microwave enzymolysis solution；Wherein, described once-combined enzyme adopts hemicellulase+protopectinase+pectinesterase hydrolytic enzyme+pectinesterase, the consumption of described hemicellulase, protopectinase, pectinesterase hydrolytic enzyme and pectinesterase is 1:3:4:6, and the 1500u/g that the enzyme concentration of once-combined enzyme is hickory nut powder raw material dosage；Microwave enzymolysis solution carrying out an alkali carry, alkali carries solid-liquid ratio 1g:25mL, and alkali carries pH10, alkali temperature raising degree 55 DEG C, and alkali carries time 4h, obtains an alkali extract, and is centrifugally separating to obtain a protein liquid and residue precipitation；Residue precipitation and water are pressed 1g:8mL mixing and prepares secondary serosity, secondary serosity adds hydrochloric acid or secondary serosity pH is adjusted to 4 by citric acid, adding secondary complex enzyme zymohydrolysis, enzyme digestion reaction temperature is 55 DEG C, and the enzyme digestion reaction time is 3 hours, and in enzymolysis process, carry out sonic oscillation process, supersonic frequency is 2KHz, and ultrasonic power is 600W, treats that enzyme digestion reaction terminates, it is warming up to 90 DEG C of enzyme denaturing 10 minutes, obtains secondary enzymolysis liquid；Wherein, described secondary compound enzyme adopts hemicellulase+saccharifying enzyme, and the consumption of described hemicellulase and saccharifying enzyme is 1:2, and the 3000u/g that the enzyme concentration of secondary compound enzyme is hickory nut powder raw material dosage；Secondary enzymolysis liquid being carried out secondary alkali carry, alkali carries solid-liquid ratio 1g:10mL, and alkali carries pH10, alkali temperature raising degree 55 DEG C, and alkali carries time 3h, obtains secondary alkali extract, and is centrifugally separating to obtain secondary protein liquid；Protein liquid and secondary protein liquid being mixed, add hydrochloric acid or Fructus Citri Limoniae acid for adjusting pH and carry out sour sinking to 4.5, the heavy temperature of acid is 20 DEG C, is centrifugally separating to obtain egg albumen powder after floccule to appear；Egg albumen powder is carried out lyophilization process, obtains Semen Caryae Cathayensis albumen；Wherein, Semen Caryae Cathayensis protein extracting ratio is 72.9%, and purity is 91.8%.
Embodiment 2:
Adopting No. six solvent soakings after being shelled by Semen Caryae Cathayensis, obtain the Semen Caryae Cathayensis dregs of rice of defat after precipitation, its resid amount is 1.7%, and is pulverized and sieved by the Semen Caryae Cathayensis dregs of rice of defat and obtain the hickory nut powder raw material that granular size is 100 mesh sieves；Hickory nut powder raw material and water are pressed solid-liquid ratio 1g:20mL mixing and prepares a serosity, adding hydrochloric acid in a serosity or a serosity pH is adjusted to 4 by citric acid, and add once-combined enzyme enzymolysis, enzyme digestion reaction temperature is 60 DEG C, the enzyme digestion reaction time is 4 hours, treat that enzyme digestion reaction terminates, be warming up to 80 DEG C of enzyme denaturing 15 minutes, obtain primary enzymolysis liquid, and primary enzymolysis liquid is carried out microwave treatment, microwave power is 250W, and the microwave time is 25 minutes, obtains microwave enzymolysis solution；Wherein, described once-combined enzyme adopts hemicellulase+protopectinase+pectinesterase hydrolytic enzyme+pectinesterase, the consumption of described hemicellulase, protopectinase, pectinesterase hydrolytic enzyme and pectinesterase is 1:6:3:4, and the 2000u/g that the enzyme concentration of once-combined enzyme is hickory nut powder raw material dosage；Microwave enzymolysis solution carrying out an alkali carry, alkali carries solid-liquid ratio 1g:20mL, and alkali carries pH9, and alkali carries temperature 50 C, and alkali carries time 3h, obtains an alkali extract, and is centrifugally separating to obtain a protein liquid and residue precipitation；Residue precipitation and water are pressed 1g:6mL mixing and prepares secondary serosity, secondary serosity adds hydrochloric acid or secondary serosity pH is adjusted to 5 by citric acid, adding secondary complex enzyme zymohydrolysis, enzyme digestion reaction temperature is 50 DEG C, and the enzyme digestion reaction time is 5 hours, and in enzymolysis process, carry out sonic oscillation process, supersonic frequency is 2KHz, and ultrasonic power is 400W, treats that enzyme digestion reaction terminates, it is warming up to 80 DEG C of enzyme denaturing 15 minutes, obtains secondary enzymolysis liquid；Wherein, described secondary compound enzyme adopts hemicellulase+saccharifying enzyme, and the consumption of described hemicellulase and saccharifying enzyme is 1:4, and the 2000u/g that the enzyme concentration of secondary compound enzyme is hickory nut powder raw material dosage；Secondary enzymolysis liquid being carried out secondary alkali carry, alkali carries solid-liquid ratio 1g:7.5mL, and alkali carries pH9, and alkali carries temperature 50 C, and alkali carries time 2h, obtains secondary alkali extract, and is centrifugally separating to obtain secondary protein liquid；Protein liquid and secondary protein liquid being mixed, add hydrochloric acid or Fructus Citri Limoniae acid for adjusting pH and carry out sour sinking to 4.5, the heavy temperature of acid is 25 DEG C, is centrifugally separating to obtain egg albumen powder after floccule to appear；Egg albumen powder is carried out lyophilization process, obtains Semen Caryae Cathayensis albumen；Wherein, Semen Caryae Cathayensis protein extracting ratio is 71.2%, and purity is 92.5%.
Embodiment 3:
Adopting No. six solvent soakings after being shelled by Semen Caryae Cathayensis, obtain the Semen Caryae Cathayensis dregs of rice of defat after precipitation, its resid amount is 2.0%, and is pulverized and sieved by the Semen Caryae Cathayensis dregs of rice of defat and obtain the hickory nut powder raw material that granular size is 150 mesh sieves；Hickory nut powder raw material and water are pressed solid-liquid ratio 1g:15mL mixing and prepares a serosity, adding hydrochloric acid in a serosity or a serosity pH is adjusted to 3 by citric acid, and add once-combined enzyme enzymolysis, enzyme digestion reaction temperature is 55 DEG C, the enzyme digestion reaction time is 8 hours, treat that enzyme digestion reaction terminates, be warming up to 85 DEG C of enzyme denaturing 20 minutes, obtain primary enzymolysis liquid, and primary enzymolysis liquid is carried out microwave treatment, microwave power is 500W, and the microwave time is 15 minutes, obtains microwave enzymolysis solution；Wherein, described once-combined enzyme adopts hemicellulase+protopectinase+pectinesterase hydrolytic enzyme+pectinesterase, the consumption of described hemicellulase, protopectinase, pectinesterase hydrolytic enzyme and pectinesterase is 1:4:2:8, and the 1000u/g that the enzyme concentration of once-combined enzyme is hickory nut powder raw material dosage；Microwave enzymolysis solution carrying out an alkali carry, alkali carries solid-liquid ratio 1g:15mL, and alkali carries pH12, and alkali carries temperature 45 C, and alkali carries time 2h, obtains an alkali extract, and is centrifugally separating to obtain a protein liquid and residue precipitation；Residue precipitation and water are pressed 1g:4mL mixing and prepares secondary serosity, secondary serosity adds hydrochloric acid or secondary serosity pH is adjusted to 4.5 by citric acid, adding secondary complex enzyme zymohydrolysis, enzyme digestion reaction temperature is 45 DEG C, and the enzyme digestion reaction time is 2 hours, and in enzymolysis process, carry out sonic oscillation process, supersonic frequency is 2KHz, and ultrasonic power is 800W, treats that enzyme digestion reaction terminates, it is warming up to 85 DEG C of enzyme denaturing 20 minutes, obtains secondary enzymolysis liquid；Wherein, described secondary compound enzyme adopts hemicellulase+saccharifying enzyme, and the consumption of described hemicellulase and saccharifying enzyme is 1:3, and the 2500u/g that the enzyme concentration of secondary compound enzyme is hickory nut powder raw material dosage；Secondary enzymolysis liquid being carried out secondary alkali carry, alkali carries solid-liquid ratio 1g:5mL, and alkali carries pH12, and alkali carries temperature 45 C, and alkali carries time 1h, obtains secondary alkali extract, and is centrifugally separating to obtain secondary protein liquid；Protein liquid and secondary protein liquid being mixed, add hydrochloric acid or Fructus Citri Limoniae acid for adjusting pH and carry out sour sinking to 4.5, the heavy temperature of acid is 30 DEG C, is centrifugally separating to obtain egg albumen powder after floccule to appear；Egg albumen powder is carried out lyophilization process, obtains Semen Caryae Cathayensis albumen；Wherein, Semen Caryae Cathayensis protein extracting ratio is 73.6%, and purity is 90.3%.
Although embodiment of the present invention are disclosed as above, but listed utilization that it is not limited in description and embodiment, it can be applied to various applicable the field of the invention completely, for those skilled in the art, it is easily achieved other amendment, therefore, under the general concept limited without departing substantially from claim and equivalency range, the present invention is not limited to specific details and shown here as the legend with description.
Claims (9)
1. the method that an enzyme process prepares Semen Caryae Cathayensis albumen, it is characterised in that comprise the steps:
Step one, hickory nut powder raw material preparation；
Step 2, primary enzymolysis: hickory nut powder raw material and water are mixed and prepares a serosity, regulate a serosity pH, and add once-combined enzyme enzymolysis, treat that enzyme digestion reaction terminates to carry out enzyme denaturing process, obtain primary enzymolysis liquid, and primary enzymolysis liquid is carried out microwave treatment, obtain microwave enzymolysis solution；
Step 3, once extraction: microwave enzymolysis solution carries out an alkali and carries, obtain an alkali extract, and be centrifugally separating to obtain a protein liquid and residue precipitation；
Step 4, secondary enzymolysis: residue precipitation and water mixing are prepared secondary serosity, regulates secondary serosity pH, add secondary complex enzyme zymohydrolysis, and in enzymolysis process, carry out sonic oscillation process, treat that enzyme digestion reaction terminates to carry out enzyme denaturing process, obtain secondary enzymolysis liquid；
Step 5, second extraction: secondary enzymolysis liquid is carried out secondary alkali and carries, obtain secondary alkali extract, and be centrifugally separating to obtain secondary protein liquid；
Step 6, acid are sunk: a protein liquid and secondary protein liquid are mixed, and carry out the heavy process of acid, be centrifugally separating to obtain egg albumen powder after regulating pH；
Step 7, lyophilization: egg albumen powder is carried out lyophilization process, obtain Semen Caryae Cathayensis albumen.
2. the method that enzyme process as claimed in claim 1 prepares Semen Caryae Cathayensis albumen, it is characterized in that, in described step one, the preparation process of hickory nut powder raw material is: adopt No. six solvent soakings after being shelled by Semen Caryae Cathayensis, the Semen Caryae Cathayensis dregs of rice of defat are obtained after precipitation, its resid amount is 1.4～2.0%, and is pulverized and sieved by the Semen Caryae Cathayensis dregs of rice of defat and obtain the hickory nut powder raw material that granular size is 100～150 mesh sieves.
3. the method that enzyme process as claimed in claim 1 prepares Semen Caryae Cathayensis albumen, it is characterized in that, in described step 2, the process of primary enzymolysis is as follows: hickory nut powder raw material and water are pressed solid-liquid ratio 1g:15～25mL mixing and prepares a serosity, a serosity adds hydrochloric acid or a serosity pH is adjusted to 3～5 by citric acid, and add once-combined enzyme enzymolysis, enzyme digestion reaction temperature is 50～60 DEG C, the enzyme digestion reaction time is 4～8 hours, treat that enzyme digestion reaction terminates, it is warming up to 80～90 DEG C of enzyme denaturing 10～20 minutes, obtain primary enzymolysis liquid, and primary enzymolysis liquid is carried out microwave treatment, microwave power is 250～500W, the microwave time is 15～25 minutes, obtain microwave enzymolysis solution.
4. the method that enzyme process as claimed in claim 3 prepares Semen Caryae Cathayensis albumen, it is characterized in that, once-combined enzyme in described step 2 adopts hemicellulase+protopectinase+pectinesterase hydrolytic enzyme+pectinesterase, the consumption of described hemicellulase, protopectinase, pectinesterase hydrolytic enzyme and pectinesterase is 1:3～6:2～4:4～8, and 1000～2000u/g that the enzyme concentration of once-combined enzyme is hickory nut powder raw material dosage.
5. the method that enzyme process as claimed in claim 1 prepares Semen Caryae Cathayensis albumen, it is characterised in that the condition once extracted in described step 3 is: alkali carries solid-liquid ratio 1g:15～25mL, and alkali carries pH9～12, and alkali temperature raising degree 45～55 DEG C, alkali carries time 2～4h.
6. the method that enzyme process as claimed in claim 1 prepares Semen Caryae Cathayensis albumen, it is characterized in that, in described step 4, the process of secondary enzymolysis is as follows: residue precipitation and water are pressed 1g:4～8mL mixing and prepares secondary serosity, secondary serosity adds hydrochloric acid or secondary serosity pH is adjusted to 4～5 by citric acid, add secondary complex enzyme zymohydrolysis, enzyme digestion reaction temperature is 45～55 DEG C, the enzyme digestion reaction time is 2～5 hours, and in enzymolysis process, carry out sonic oscillation process, supersonic frequency is 2KHz, ultrasonic power is 400～800W, treat that enzyme digestion reaction terminates, it is warming up to 80～90 DEG C of enzyme denaturing 10～20 minutes, obtain secondary enzymolysis liquid.
7. the method that enzyme process as claimed in claim 6 prepares Semen Caryae Cathayensis albumen, it is characterized in that, secondary compound enzyme in described step 4 adopts hemicellulase+saccharifying enzyme, the consumption of described hemicellulase and saccharifying enzyme is 1:2～4, and 2000～3000u/g that the enzyme concentration of secondary compound enzyme is hickory nut powder raw material dosage.
8. the method that enzyme process as claimed in claim 1 prepares Semen Caryae Cathayensis albumen, it is characterised in that in described step 5, the condition of second extraction is: alkali carries solid-liquid ratio 1g:5～10mL, and alkali carries pH9～12, and alkali temperature raising degree 45～55 DEG C, alkali carries time 1～3h.
9. the method that enzyme process as claimed in claim 1 prepares Semen Caryae Cathayensis albumen, it is characterized in that, in described step 6, the heavy process of acid is: a protein liquid and secondary protein liquid are mixed, add hydrochloric acid or Fructus Citri Limoniae acid for adjusting pH and carry out sour sinking to 4.5, the heavy temperature of acid is 20～30 DEG C, is centrifugally separating to obtain egg albumen powder after floccule to appear.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610152003.6A CN105767455A (en) | 2016-03-17 | 2016-03-17 | Method for preparing hickory nut protein by enzyme method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610152003.6A CN105767455A (en) | 2016-03-17 | 2016-03-17 | Method for preparing hickory nut protein by enzyme method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105767455A true CN105767455A (en) | 2016-07-20 |
Family
ID=56392959
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610152003.6A Pending CN105767455A (en) | 2016-03-17 | 2016-03-17 | Method for preparing hickory nut protein by enzyme method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105767455A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106805180A (en) * | 2017-01-13 | 2017-06-09 | 江南大学 | A kind of method that polyphenol substance is extracted in desmoenzyme and the ultrasonically treated endotesta walnut kernel by band |
| CN106916200A (en) * | 2017-05-11 | 2017-07-04 | 武汉轻工大学 | A kind of method that alkali extraction-acid precipitation prepares rubber seed albumen |
| CN109055469A (en) * | 2018-08-29 | 2018-12-21 | 武汉轻工大学 | A kind of preparation method of pumpkin albumen |
| CN109645212A (en) * | 2019-01-22 | 2019-04-19 | 武汉轻工大学 | A kind of preparation method of sesame protein |
| CN113699206A (en) * | 2021-08-27 | 2021-11-26 | 浙江农林大学 | Method for preparing hickory protein by using hickory cake meal |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103183721A (en) * | 2011-12-27 | 2013-07-03 | 东北林业大学 | Process condition for extracting proteins of Northeast China carya cathayensis mandshurica seeds |
| CN105063149A (en) * | 2015-09-01 | 2015-11-18 | 武汉轻工大学 | Method for auxiliary preparation of cottonseed protein by means of enzymatic method |
-
2016
- 2016-03-17 CN CN201610152003.6A patent/CN105767455A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103183721A (en) * | 2011-12-27 | 2013-07-03 | 东北林业大学 | Process condition for extracting proteins of Northeast China carya cathayensis mandshurica seeds |
| CN105063149A (en) * | 2015-09-01 | 2015-11-18 | 武汉轻工大学 | Method for auxiliary preparation of cottonseed protein by means of enzymatic method |
Non-Patent Citations (1)
| Title |
|---|
| 杨瑾，等: "山核桃饼粕分离蛋白的提取工艺研究", 《食品工业科技》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106805180A (en) * | 2017-01-13 | 2017-06-09 | 江南大学 | A kind of method that polyphenol substance is extracted in desmoenzyme and the ultrasonically treated endotesta walnut kernel by band |
| CN106805180B (en) * | 2017-01-13 | 2020-08-07 | 江南大学 | Method for extracting polyphenol substance from walnut kernel with inner seed coat by combining enzyme and ultrasonic treatment |
| CN106916200A (en) * | 2017-05-11 | 2017-07-04 | 武汉轻工大学 | A kind of method that alkali extraction-acid precipitation prepares rubber seed albumen |
| CN109055469A (en) * | 2018-08-29 | 2018-12-21 | 武汉轻工大学 | A kind of preparation method of pumpkin albumen |
| CN109645212A (en) * | 2019-01-22 | 2019-04-19 | 武汉轻工大学 | A kind of preparation method of sesame protein |
| CN113699206A (en) * | 2021-08-27 | 2021-11-26 | 浙江农林大学 | Method for preparing hickory protein by using hickory cake meal |
| CN113699206B (en) * | 2021-08-27 | 2023-12-15 | 浙江农林大学 | Method for preparing hickory nut protein by using hickory nut cake |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105767455A (en) | Method for preparing hickory nut protein by enzyme method | |
| CN102845707B (en) | Production technology for producing nutrient seasoning powder by enzymatic method with comprehensive utilization of avian skeleton | |
| CN105560097B (en) | A kind of fresh peony flower ferment and its preparation method and application | |
| CN105586377B (en) | A kind of enzymolysis processing method of seaweed | |
| CN105779542A (en) | Method for preparing organic rapeseed polypeptide through enzyme method | |
| CN107794288A (en) | A kind of Queensland nut polypeptide ultrasonic wave added complex enzyme hydrolysis extraction process | |
| CN106086133A (en) | A kind of enzyme process prepares the method for Semen coryli heterophyllae albumen | |
| CN107772076B (en) | Cordyceps sinensis based feed immunopotentiator and preparation method thereof | |
| CN107821730A (en) | A kind of Queensland nut polypeptide ultrasound assisted extraction technique | |
| CN102845711A (en) | Production technology for producing nutrient seasoning paste by enzymatic method using avian skeleton | |
| CN103981244A (en) | Method of preparing hypoallergenic soybean protein | |
| CN109111497A (en) | A kind of method for producing of peony seeds albumen | |
| CN105063149B (en) | A kind of method that enzyme process auxiliary prepares cottonseed protein | |
| CN107814829A (en) | A kind of method of combination of ultrasound electric field treatment assisted extraction Queensland nut polypeptide | |
| KR100887544B1 (en) | Method for producing onion liquor | |
| WO1995018626A1 (en) | A tonic drug made from basidiomycetes, algae, chinese herbal medicine and the method thereof | |
| CN102845708B (en) | Production technology for producing nutrient seasoning powder by biological enzyme techniques using avian skeleton | |
| CN108752417A (en) | A kind of pollen pini active peptides | |
| CN105795003A (en) | Carya cathayensis oil preparation method using aqueous enzymatic method | |
| CN103918473A (en) | Animal feed production method | |
| CN105779543A (en) | Method for preparing organic rapeseed protein through enzyme method | |
| CN107581512A (en) | A kind of preparation method of soluble Penis et testis cervi extract | |
| CN104905208A (en) | Poultry bone condiment for tonifying kidney and enriching brain | |
| CN105725084B (en) | Production method for preparing low-purine defatted soybean powder by using malt root powder | |
| CN107574066A (en) | A kind of polypeptide Flower wine and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160720 |
|
| RJ01 | Rejection of invention patent application after publication |