CN106916200A - A kind of method that alkali extraction-acid precipitation prepares rubber seed albumen - Google Patents
A kind of method that alkali extraction-acid precipitation prepares rubber seed albumen Download PDFInfo
- Publication number
- CN106916200A CN106916200A CN201710327968.9A CN201710327968A CN106916200A CN 106916200 A CN106916200 A CN 106916200A CN 201710327968 A CN201710327968 A CN 201710327968A CN 106916200 A CN106916200 A CN 106916200A
- Authority
- CN
- China
- Prior art keywords
- rubber seed
- precipitation
- albumen
- slurries
- alkali
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a kind of method that alkali extraction-acid precipitation prepares rubber seed albumen, comprise the following steps：The preparation of step one, rubber seed powder raw material；Step 2, an alkali soluble：Obtain an alkali soluble precipitation and a rubber seed protein solution；Step 3, secondary alkali soluble：Obtain secondary alkali soluble precipitation and secondary rubber seed protein solution；Step 4, three alkali solubles：Obtain three rubber seed protein solutions；Step 5, acid are heavy：Obtain rubber seed albumen powder；Step 6, purifying：Obtain purified rubber seed albumen powder；Step 7, freeze-drying：Purified rubber seed albumen powder is carried out into freeze-drying process, rubber seed albumen is obtained final product.The method that the alkali extraction-acid precipitation prepares rubber seed albumen, process safety, to prepare convenient, rubber seed protein extracting ratio high.
Description
Technical field
The present invention relates to rubber seed oil technical field, and in particular to a kind of alkali extraction-acid precipitation prepares the side of rubber seed albumen
Method.
Background technology
Rubber seed (rubber seed), the oval seed of Euphorbiaceae arbor Brazil Hevea rubber capsule, in oval,
Brown simultaneously carries silver gray speckle, is made up of kind of shell and benevolence, and inside kind of shell, appearance is surrounded by the film layer of softness to benevolence, is
A kind of tropical xylophyta oil plant.Rubber seed shells the part after oil expression for the rubber seed dregs of rice, and 60% carbon is about contained in the rubber seed dregs of rice
Hydrate, 25% crude protein, 28% amino acid.Rubber seed albumen is complete protein, containing 18 kinds of amino acid, wherein 8
Middle essential amino acid is complete, and the content of arginine, glutamic acid and asparagus fern door propylhomoserin is high, and most of rubber seed albumen is hydrophobicity ammonia
Base acid and acidic amino acid, with edibility and healthcare function very high.Therefore, it is necessary to studying one kind prepares rubber seed egg
White method.
The content of the invention
For weak point present in above-mentioned technology, the invention provides a kind of process safety, convenient, rubber seed is prepared
The method that protein extracting ratio alkali extraction-acid precipitation high prepares rubber seed albumen.
The technical solution adopted for the present invention to solve the technical problems is：A kind of alkali extraction-acid precipitation prepares rubber seed albumen
Method, comprises the following steps：The preparation of step one, rubber seed powder raw material；Step 2, an alkali soluble：By rubber seed powder raw material and
Water mixes a prepared slurries by a certain percentage, adjusts a slurries pH and carries out ultrasonication to it, obtains an alkali soluble
Liquid, by an aqueous slkali centrifugation, obtains an alkali soluble precipitation and a rubber seed protein solution；Step 3, secondary alkali soluble：
Alkali soluble precipitation and water are mixed into prepared secondary slurries by a certain percentage, secondary slurries pH is adjusted and it is carried out at ultrasonic wave
Reason, obtains secondary aqueous slkali, by secondary aqueous slkali centrifugation, obtains secondary alkali soluble precipitation and secondary rubber seed protein solution；
Step 4, three alkali solubles：Secondary alkali soluble precipitation and water are mixed into prepared three slurries by a certain percentage, three slurries pH of regulation are simultaneously
Ultrasonication is carried out to it, three aqueous slkalis are obtained, by three aqueous slkali centrifugations, three rubber seed albumen is obtained molten
Liquid；Step 5, acid are heavy：Rubber seed protein solution, secondary rubber seed protein solution and three rubber seed protein solutions are mixed
It is even, adjust its pH and carry out the heavy treatment of acid, it is centrifuged obtaining rubber seed albumen powder；Step 6, purifying：By rubber seed albumen
Powder and water mix prepared rubber seed protein slurry by a certain percentage, adjust rubber seed protein slurry pH and add complex enzyme to be surpassed
Sound assistance enzymolysis, after digesting, destroy the enzyme treatment are carried out by enzymolysis liquid, are centrifuged obtaining albumen precipitation, wash albumen precipitation
PH obtains purified rubber seed albumen powder to neutrality；Step 7, freeze-drying：Purified rubber seed albumen powder is carried out into freezing dry
Dry treatment, obtains final product rubber seed albumen.
Preferably, the preparation process of rubber seed powder is in the step one：Using No. six solvent leachings after rubber seed is shelled
Bubble, obtains the rubber seed dregs of rice of degreasing after precipitation, its resid amount is 0.8~1.2%, and the rubber seed dregs of rice of degreasing are pulverized and sieved
To the rubber seed powder raw material that granular size is 80~100 mesh sieves.
Preferably, the process of an alkali soluble is as follows in the step 2：Rubber seed powder raw material and water are pressed into solid-liquid ratio 1g:20
~25mL is mixed and a slurries is obtained, and to adding sodium hydroxide solution in a slurries, slurries pH is adjusted into 10~11, and
Ultrasonication is carried out to it, ultrasonic power is 20~40kHz, and ultrasonic time is 80~100 minutes, and ultrasonic temperature is 54~56
DEG C, an aqueous slkali is obtained, by an aqueous slkali centrifugation, obtain an alkali soluble precipitation and a rubber seed protein solution.
Preferably, the process of secondary alkali soluble is as follows in the step 3：Alkali soluble precipitation and water are pressed into solid-liquid ratio 1g:15
~20mL is mixed and secondary slurries is obtained, and to adding sodium hydroxide solution in secondary slurries, secondary slurries pH is adjusted into 11~12, and
Ultrasonication is carried out to it, ultrasonic power is 20~40kHz, and ultrasonic time is 60~80 minutes, and ultrasonic temperature is 48~52
DEG C, secondary aqueous slkali is obtained, by secondary aqueous slkali centrifugation, obtain secondary alkali soluble precipitation and secondary rubber seed protein solution.
Preferably, the process of three alkali solubles is as follows in the step 4：Secondary alkali soluble precipitation and water are pressed into solid-liquid ratio 1g:10
~15mL is mixed and three slurries is obtained, and to adding sodium hydroxide solution in three slurries, secondary slurries pH is adjusted into 12~13, and
Ultrasonication is carried out to it, ultrasonic power is 20~40kHz, and ultrasonic time is 40~60 minutes, and ultrasonic temperature is 44~46
DEG C, three aqueous slkalis are obtained, by three aqueous slkali centrifugations, obtain three rubber seed protein solutions.
Preferably, the heavy process of acid is as follows in the step 5：By rubber seed protein solution, a secondary rubber seed albumen
Solution and three rubber seed protein solutions are mixed, and acid is heavy to be processed to add hydrochloric acid or lemon acid for adjusting pH to be carried out to 4.5, the heavy temperature of acid
It it is 40~50 DEG C, through being centrifugally separating to obtain rubber seed albumen powder after floccule to appear.
Preferably, the process for being purified in the step 6 is as follows：Rubber seed albumen powder and water are pressed into solid-liquid ratio 1g:8~
10mL is mixed and rubber seed protein slurry is obtained, and rubber seed protein slurry pH is to 4~5 for regulation, and it is auxiliary to add complex enzyme to carry out ultrasound
Enzymolysis is helped, ultrasonic power is 20~40kHz, and ultrasonic time is 60~80min, and ultrasonic temperature is 40~50 DEG C, after digesting, will
Enzymolysis liquid is heated to 85~95 DEG C of destroy the enzyme treatments 10~15 minutes, is centrifuged obtaining albumen precipitation, using 35~45 DEG C
Distilled water washes albumen precipitation pH to 7, obtains purified rubber seed albumen powder.
Preferably, the complex enzyme uses cellulase+amylase+carbohydrase+pectinesterase hydrolase, the cellulose
Enzyme：Amylase：Carbohydrase：The consumption of pectinesterase hydrolase is 1：3~6：4~8：2~4, and the enzyme concentration of complex enzyme is rubber
1.5~2.5ug/g of glue seed protein slurry consumption.
Preferably, the cellulase：Amylase：Carbohydrase：The consumption of pectinesterase hydrolase is 1：4.5：6：3, and
The enzyme concentration of complex enzyme is 2 ug/g of rubber seed protein slurry consumption.
Compared with prior art, its advantage is the present invention：The alkali extraction-acid precipitation that the present invention is provided prepares rubber seed egg
White method, by adjusting the pH value in alkali soluble, secondary alkali soluble, three alkali soluble methods successively, can abundant dissolving rubber seed
Protein ingredient in powder raw material, so as to effectively improve the recovery rate of rubber seed albumen；Use a certain proportion of by purifying
The complex enzyme of cellulase+amylase+carbohydrase+pectinesterase hydrolase composition is digested, and can effectively remove some carbohydrates
Deng false albumen, to increase the purity of rubber seed albumen；Albumen is washed by the distilled water using 35~45 DEG C after destroy the enzyme treatment
Precipitation pH to 7, can reduce the damage and redissolution to rubber seed albumen；The method that the alkali extraction-acid precipitation prepares rubber seed albumen,
Process safety, prepare convenient, rubber seed protein extracting ratio up to more than 75.0%, the rubber seed purity of protein that extracts be up to 90.0%
More than.
Brief description of the drawings
Fig. 1 is the method flow diagram that alkali extraction-acid precipitation of the present invention prepares rubber seed albumen.
Specific embodiment
The present invention is described in further detail below in conjunction with the accompanying drawings, to make those skilled in the art with reference to specification text
Word can be implemented according to this.
As shown in figure 1, the invention provides a kind of method that alkali extraction-acid precipitation prepares rubber seed albumen, including following step
Suddenly：
The preparation of step one, rubber seed powder raw material：No. six solvent soakings are used after rubber seed is shelled, degreasing is obtained after precipitation
The rubber seed dregs of rice, its resid amount is 0.8~1.2%, and the rubber seed dregs of rice of degreasing are pulverized and sieved obtains granular size for 80~100
The rubber seed powder raw material of mesh sieve；
Step 2, rubber seed powder raw material and water are pressed into solid-liquid ratio 1g:20~25mL is mixed and a slurries is obtained, in a slurries
Sodium hydroxide solution is added, a slurries pH 10~11 is adjusted to, and ultrasonication is carried out to it, ultrasonic power is 20~
40kHz, ultrasonic time be 80~100 minutes, ultrasonic temperature be 54~56 DEG C, obtain an aqueous slkali, by an aqueous slkali from
The heart is separated, and obtains an alkali soluble precipitation and a rubber seed protein solution；
Step 3, alkali soluble precipitation and water are pressed into solid-liquid ratio 1g:15~20mL is mixed and secondary slurries is obtained, in secondary slurries
Sodium hydroxide solution is added, secondary slurries pH 11~12 is adjusted to, and ultrasonication is carried out to it, ultrasonic power is 20~
40kHz, ultrasonic time is 60~80 minutes, and ultrasonic temperature is 48~52 DEG C, obtains secondary aqueous slkali, by the centrifugation of secondary aqueous slkali
Separate, obtain secondary alkali soluble precipitation and secondary rubber seed protein solution；
Step 4, by secondary alkali soluble precipitation and water press solid-liquid ratio 1g:10~15mL is mixed and three slurries is obtained, in three slurries
Sodium hydroxide solution is added, secondary slurries pH 12~13 is adjusted to, and ultrasonication is carried out to it, ultrasonic power is 20~
40kHz, ultrasonic time is 40~60 minutes, and ultrasonic temperature is 44~46 DEG C, obtains three aqueous slkalis, and three aqueous slkalis are centrifuged
Separate, obtain three rubber seed protein solutions；
Step 5, rubber seed protein solution, secondary rubber seed protein solution and three rubber seed protein solutions are mixed, plus
Entering hydrochloric acid or lemon acid for adjusting pH carries out the heavy treatment of acid to 4.5, and the heavy temperature of acid is 40~50 DEG C, through centrifugation after floccule to appear
Isolated rubber seed albumen powder；
Step 6, rubber seed albumen powder and water are pressed into solid-liquid ratio 1g:8~10mL is mixed and rubber seed protein slurry is obtained, and adjusts rubber
Glue seed protein slurry pH adds the complex enzyme to carry out ultrasonic wave added enzymolysis to 4~5, and the complex enzyme uses cellulase+starch
Enzyme+carbohydrase+pectinesterase hydrolase, the cellulase：Amylase：Carbohydrase：The consumption of pectinesterase hydrolase is 1：3
~6：4~8：2~4, and the enzyme concentration of complex enzyme is 1.5~2.5ug/g of rubber seed protein slurry consumption, ultrasonic power is 20
~40kHz, ultrasonic time is 60~80min, and ultrasonic temperature is 40~50 DEG C, after digesting, enzymolysis liquid is heated into 85~95
DEG C destroy the enzyme treatment 10~15 minutes, is centrifuged obtaining albumen precipitation, and albumen precipitation is washed using 35~45 DEG C of distilled water
PH obtains purified rubber seed albumen powder to 7；Preferably, the cellulase：Amylase：Carbohydrase：Pectinesterase hydrolase
Consumption is 1：4.5：6：3, and the enzyme concentration of complex enzyme is 2 ug/g of rubber seed protein slurry consumption；
Step 7, purified rubber seed albumen powder is carried out into freeze-drying process, obtain final product rubber seed albumen.
Embodiment 1
Rubber seed powder raw material and water are pressed into solid-liquid ratio 1g:20mL is mixed and a slurries is obtained, and a slurries pH is adjusted into 10, and right
It carries out ultrasonication, obtains an aqueous slkali, by an aqueous slkali centrifugation, obtains an alkali soluble precipitation and a rubber
Glue seed protein solution；Alkali soluble precipitation and water are pressed into solid-liquid ratio 1g:15mL is mixed and secondary slurries is obtained, and secondary slurries pH is adjusted
To 11, and ultrasonication is carried out to it, obtain secondary aqueous slkali, by secondary aqueous slkali centrifugation, obtained secondary alkali soluble and sink
Form sediment and secondary rubber seed protein solution；Secondary alkali soluble precipitation and water are pressed into solid-liquid ratio 1g:10mL is mixed and three slurries is obtained, by two
Secondary slurries pH is adjusted to 12, and carries out ultrasonication to it, obtains three aqueous slkalis, and three aqueous slkali centrifugations are obtained
Three rubber seed protein solutions；Rubber seed protein solution, secondary rubber seed protein solution and three rubber seed albumen are molten
Liquid is mixed, and regulation pH to 4.5 carries out the heavy treatment of acid, and the heavy temperature of acid is 40 DEG C, through being centrifugally separating to obtain rubber after floccule to appear
Glue seed albumen powder；Rubber seed albumen powder and water are pressed into solid-liquid ratio 1g:8mL is mixed and rubber seed protein slurry is obtained, and adjusts rubber seed
Protein slurry pH adds the complex enzyme to carry out ultrasonic wave added enzymolysis to 4, and the complex enzyme uses cellulase+amylase+saccharification
Enzyme+pectinesterase hydrolase, the cellulase：Amylase：Carbohydrase：The consumption of pectinesterase hydrolase is 1：3：4：2, and
The enzyme concentration of complex enzyme is the 1.5ug/g of rubber seed protein slurry consumption, after digesting, enzymolysis liquid is heated at 85 DEG C of enzymes that go out
Reason 10 minutes, is centrifuged obtaining albumen precipitation, and albumen precipitation pH is washed to 7 using 35 DEG C of distilled water, obtains purifying rubber
Glue seed albumen powder；Purified rubber seed albumen powder is carried out into freeze-drying process, rubber seed albumen is obtained final product, wherein, rubber seed albumen
Recovery rate is 75.2%, and purity is 93.6%.
Embodiment 2
Rubber seed powder raw material and water are pressed into solid-liquid ratio 1g:25mL is mixed and a slurries is obtained, and slurries pH is adjusted into 11, and
Ultrasonication is carried out to it, an aqueous slkali is obtained, by an aqueous slkali centrifugation, alkali soluble precipitation and once is obtained
Rubber seed protein solution；Alkali soluble precipitation and water are pressed into solid-liquid ratio 1g:20mL is mixed and secondary slurries is obtained, by secondary slurries
PH is adjusted to 12, and carries out ultrasonication to it, obtains secondary aqueous slkali, by secondary aqueous slkali centrifugation, obtains secondary alkali
Molten precipitation and secondary rubber seed protein solution；Secondary alkali soluble precipitation and water are pressed into solid-liquid ratio 1g:15mL is mixed and three slurries is obtained
Liquid, 13 are adjusted to by secondary slurries pH, and carry out ultrasonication to it, obtain three aqueous slkalis, by three aqueous slkali centrifugations point
From obtaining three rubber seed protein solutions；By rubber seed protein solution, secondary rubber seed protein solution and three rubber seeds
Protein solution is mixed, and regulation pH to 4.5 carries out the heavy treatment of acid, and the heavy temperature of acid is 50 DEG C, through centrifugation after floccule to appear
Obtain rubber seed albumen powder；Rubber seed albumen powder and water are pressed into solid-liquid ratio 1g:10mL is mixed and rubber seed protein slurry is obtained, and is adjusted
Section rubber seed protein slurry pH adds the complex enzyme to carry out ultrasonic wave added enzymolysis to 5, and the complex enzyme uses cellulase+shallow lake
Powder enzyme+carbohydrase+pectinesterase hydrolase, the cellulase：Amylase：Carbohydrase：The consumption of pectinesterase hydrolase is
1：6：8：4, and the enzyme concentration of complex enzyme is the 2.5ug/g of rubber seed protein slurry consumption, after digesting, enzymolysis liquid is heated to
95 DEG C of destroy the enzyme treatments 15 minutes, are centrifuged obtaining albumen precipitation, and albumen precipitation pH is washed to 7 using 45 DEG C of distilled water,
Obtain purified rubber seed albumen powder；Purified rubber seed albumen powder is carried out into freeze-drying process, rubber seed albumen is obtained final product, wherein,
Rubber seed protein extracting ratio is 76.8%, and purity is 92.1%.
Embodiment 3
Rubber seed powder raw material and water are pressed into solid-liquid ratio 1g:22.5mL is mixed and a slurries is obtained, and a slurries pH is adjusted into 10.5,
And ultrasonication is carried out to it, and an aqueous slkali is obtained, by an aqueous slkali centrifugation, obtain an alkali soluble precipitation and one
Secondary rubber seed protein solution；Alkali soluble precipitation and water are pressed into solid-liquid ratio 1g:17.5mL is mixed and secondary slurries is obtained, by secondary slurry
Liquid pH is adjusted to 11.5, and carries out ultrasonication to it, obtains secondary aqueous slkali, by secondary aqueous slkali centrifugation, obtains two
Secondary alkali soluble precipitation and secondary rubber seed protein solution；Secondary alkali soluble precipitation and water are pressed into solid-liquid ratio 1g:12.5mL is mixed and is obtained three
Secondary slurries, 12.5 are adjusted to by secondary slurries pH, and carry out ultrasonication to it, obtain three aqueous slkalis, by three aqueous slkalis
Centrifugation, obtains three rubber seed protein solutions；By rubber seed protein solution, secondary rubber seed protein solution and three times
Rubber seed protein solution is mixed, and regulation pH to 4.5 carries out the heavy treatment of acid, and the heavy temperature of acid is 45 DEG C, passed through after floccule to appear from
The isolated rubber seed albumen powder of the heart；Rubber seed albumen powder and water are pressed into solid-liquid ratio 1g:9mL is mixed and rubber seed protein milk is obtained
Liquid, rubber seed protein slurry pH is to 4.5 for regulation, and adds the complex enzyme to carry out ultrasonic wave added enzymolysis, and the complex enzyme uses fiber
Plain enzyme+amylase+carbohydrase+pectinesterase hydrolase, the cellulase：Amylase：Carbohydrase：Pectinesterase hydrolase
Consumption is 1：4.5：6：3, and the enzyme concentration of complex enzyme is 2 ug/g of rubber seed protein slurry consumption, after digesting, will be digested
Liquid is heated to 90 DEG C of destroy the enzyme treatments 12.5 minutes, is centrifuged obtaining albumen precipitation, and albumen is washed using 40 DEG C of distilled water
PH is to 7 for precipitation, obtains purified rubber seed albumen powder；Purified rubber seed albumen powder is carried out into freeze-drying process, rubber seed is obtained final product
Albumen, wherein, rubber seed protein extracting ratio is 77.2%, and purity is 94.5%.
Although embodiment of the present invention is disclosed as above, it is not limited in listed fortune in specification and implementation method
With, it can be applied to various suitable the field of the invention completely, for those skilled in the art, can be easily real
Now other modification, therefore under the universal limited without departing substantially from claim and equivalency range, the present invention is not limited to
Specific details and shown here as the legend with description.
Claims (9)
1. a kind of method that alkali extraction-acid precipitation prepares rubber seed albumen, it is characterised in that comprise the following steps：
The preparation of step one, rubber seed powder raw material；
Step 2, an alkali soluble：Rubber seed powder raw material and water are mixed into a prepared slurries by a certain percentage, a slurries are adjusted
PH simultaneously carries out ultrasonication to it, obtains an aqueous slkali, by an aqueous slkali centrifugation, obtain alkali soluble precipitation and
Rubber seed protein solution；
Step 3, secondary alkali soluble：Alkali soluble precipitation and water are mixed into prepared secondary slurries by a certain percentage, secondary slurries are adjusted
PH simultaneously carries out ultrasonication to it, obtains secondary aqueous slkali, by secondary aqueous slkali centrifugation, obtain secondary alkali soluble precipitation and
Secondary rubber seed protein solution；
Step 4, three alkali solubles：Secondary alkali soluble precipitation and water are mixed into prepared three slurries by a certain percentage, three slurries are adjusted
PH simultaneously carries out ultrasonication to it, obtains three aqueous slkalis, by three aqueous slkali centrifugations, obtains three rubber seed albumen
Solution；
Step 5, acid are heavy：Rubber seed protein solution, secondary rubber seed protein solution and three rubber seed protein solutions are mixed
It is even, adjust its pH and carry out the heavy treatment of acid, it is centrifuged obtaining rubber seed albumen powder；
Step 6, purifying：Rubber seed albumen powder and water are mixed into prepared rubber seed protein slurry by a certain percentage, rubber seed is adjusted
Protein slurry pH simultaneously adds the complex enzyme to carry out ultrasonic wave added enzymolysis, after digesting, enzymolysis liquid is carried out into destroy the enzyme treatment, through centrifugation point
From albumen precipitation is obtained, albumen precipitation pH is to neutrality for washing, that is, obtain purified rubber seed albumen powder；
Step 7, freeze-drying：Purified rubber seed albumen powder is carried out into freeze-drying process, rubber seed albumen is obtained final product.
2. the method that alkali extraction-acid precipitation as claimed in claim 1 prepares rubber seed albumen, it is characterised in that in the step one
The preparation process of rubber seed powder is：No. six solvent soakings are used after rubber seed is shelled, the rubber seed dregs of rice of degreasing are obtained after precipitation,
Its resid amount is 0.8~1.2%, and the rubber seed dregs of rice of degreasing pulverize and sieve the rubber for obtaining that granular size is 80~100 mesh sieves
Seed powder raw material.
3. the method that alkali extraction-acid precipitation as claimed in claim 1 prepares rubber seed albumen, it is characterised in that in the step 2
The process of alkali soluble is as follows：Rubber seed powder raw material and water are pressed into solid-liquid ratio 1g:20~25mL is mixed and a slurries, Xiang Yi is obtained
Sodium hydroxide solution is added in secondary slurries, a slurries pH 10~11 is adjusted to, and ultrasonication, ultrasonic power are carried out to it
It is 20~40kHz, ultrasonic time is 80~100 minutes, and ultrasonic temperature is 54~56 DEG C, an aqueous slkali is obtained, by an alkali
Solution centrifugal is separated, and obtains an alkali soluble precipitation and a rubber seed protein solution.
4. the method that alkali extraction-acid precipitation as claimed in claim 1 prepares rubber seed albumen, it is characterised in that in the step 3
The process of secondary alkali soluble is as follows：Alkali soluble precipitation and water are pressed into solid-liquid ratio 1g:15~20mL is mixed and secondary slurries is obtained, to two
Sodium hydroxide solution is added in secondary slurries, secondary slurries pH 11~12 is adjusted to, and ultrasonication, ultrasonic power are carried out to it
It is 20~40kHz, ultrasonic time is 60~80 minutes, and ultrasonic temperature is 48~52 DEG C, secondary aqueous slkali is obtained, by secondary alkali soluble
Liquid centrifugation, obtains secondary alkali soluble precipitation and secondary rubber seed protein solution.
5. the method that alkali extraction-acid precipitation as claimed in claim 1 prepares rubber seed albumen, it is characterised in that in the step 4
The process of three alkali solubles is as follows：Secondary alkali soluble precipitation and water are pressed into solid-liquid ratio 1g:10~15mL is mixed and three slurries is obtained, to three
Sodium hydroxide solution is added in secondary slurries, secondary slurries pH 12~13 is adjusted to, and ultrasonication, ultrasonic power are carried out to it
It is 20~40kHz, ultrasonic time is 40~60 minutes, and ultrasonic temperature is 44~46 DEG C, three aqueous slkalis is obtained, by three alkali solubles
Liquid centrifugation, obtains three rubber seed protein solutions.
6. the method that alkali extraction-acid precipitation as claimed in claim 1 prepares rubber seed albumen, it is characterised in that in the step 5
The heavy process of acid is as follows：Rubber seed protein solution, secondary rubber seed protein solution and three rubber seed protein solutions are mixed
Even, acid is heavy to be processed to add hydrochloric acid or lemon acid for adjusting pH to be carried out to 4.5, and the heavy temperature of acid is 40~50 DEG C, after floccule to appear
It is centrifuged obtaining rubber seed albumen powder.
7. the method that alkali extraction-acid precipitation as claimed in claim 1 prepares rubber seed albumen, it is characterised in that in the step 6
The process of purifying is as follows：Rubber seed albumen powder and water are pressed into solid-liquid ratio 1g:8~10mL is mixed and rubber seed protein slurry is obtained, and is adjusted
Section rubber seed protein slurry pH adds the complex enzyme to carry out ultrasonic wave added enzymolysis to 4~5, and ultrasonic power is 20~40kHz, is surpassed
The sound time is 60~80min, and ultrasonic temperature is 40~50 DEG C, after digesting, enzymolysis liquid is heated into 85~95 DEG C of destroy the enzyme treatments
10~15 minutes, it is centrifuged obtaining albumen precipitation, albumen precipitation pH is washed to 7 using 35~45 DEG C of distilled water, obtains
Purified rubber seed albumen powder.
8. the method that alkali extraction-acid precipitation as claimed in claim 7 prepares rubber seed albumen, it is characterised in that the complex enzyme is adopted
With cellulase+amylase+carbohydrase+pectinesterase hydrolase, the cellulase：Amylase：Carbohydrase：Pectinesterase water
The consumption for solving enzyme is 1：3~6：4~8：2~4, and complex enzyme enzyme concentration for rubber seed protein slurry consumption 1.5~
2.5ug/g。
9. the method that alkali extraction-acid precipitation as claimed in claim 8 prepares rubber seed albumen, it is characterised in that the cellulose
Enzyme：Amylase：Carbohydrase：The consumption of pectinesterase hydrolase is 1：4.5：6：3, and the enzyme concentration of complex enzyme is rubber seed albumen
2 ug/g of slurries consumption.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710327968.9A CN106916200A (en) | 2017-05-11 | 2017-05-11 | A kind of method that alkali extraction-acid precipitation prepares rubber seed albumen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710327968.9A CN106916200A (en) | 2017-05-11 | 2017-05-11 | A kind of method that alkali extraction-acid precipitation prepares rubber seed albumen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106916200A true CN106916200A (en) | 2017-07-04 |
Family
ID=59568284
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710327968.9A Pending CN106916200A (en) | 2017-05-11 | 2017-05-11 | A kind of method that alkali extraction-acid precipitation prepares rubber seed albumen |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106916200A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112266405A (en) * | 2020-10-27 | 2021-01-26 | 赵博 | Method for recovering protein from potato starch processing wastewater |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105063149A (en) * | 2015-09-01 | 2015-11-18 | 武汉轻工大学 | Method for auxiliary preparation of cottonseed protein by means of enzymatic method |
| CN105779543A (en) * | 2016-04-13 | 2016-07-20 | 武汉轻工大学 | Method for preparing organic rapeseed protein through enzyme method |
| CN105767455A (en) * | 2016-03-17 | 2016-07-20 | 武汉轻工大学 | Method for preparing hickory nut protein by enzyme method |
-
2017
- 2017-05-11 CN CN201710327968.9A patent/CN106916200A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105063149A (en) * | 2015-09-01 | 2015-11-18 | 武汉轻工大学 | Method for auxiliary preparation of cottonseed protein by means of enzymatic method |
| CN105767455A (en) * | 2016-03-17 | 2016-07-20 | 武汉轻工大学 | Method for preparing hickory nut protein by enzyme method |
| CN105779543A (en) * | 2016-04-13 | 2016-07-20 | 武汉轻工大学 | Method for preparing organic rapeseed protein through enzyme method |
Non-Patent Citations (1)
| Title |
|---|
| 杨杰等: "橡胶籽蛋白提取工艺研究", 《中国油脂》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112266405A (en) * | 2020-10-27 | 2021-01-26 | 赵博 | Method for recovering protein from potato starch processing wastewater |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102150737B (en) | Method for preparing high-purity rice protein and acquired products by using same | |
| CN101946891B (en) | Method for preparing water-soluble dietary fiber of corn peels | |
| CN102224879B (en) | Preparation method and application of champignon polypeptide | |
| CN103283934A (en) | Preparation method of high-purity rice protein | |
| CN106086133B (en) | A kind of method that enzyme process prepares fibert albumen | |
| JP2007068454A (en) | Method for producing rice protein, rice protein produced by the method, and food | |
| CN103478400A (en) | High-purity rice bran protein and rice bran fibers and preparation method thereof | |
| CN110734948A (en) | extraction device and process for extracting selenium polypeptide from soybeans | |
| CN1974602B (en) | Production technology of extracting oat starch and oat protein powder from oat | |
| CN109055469A (en) | A kind of preparation method of pumpkin albumen | |
| CN103497986B (en) | Enzyme-method rice starch and rice protein co-production process | |
| CN106349405A (en) | Method for extracting pectin from shaddock peel through enzymolysis and ultrasonic waves | |
| CN104262491A (en) | Method for extracting high-purity starch by rice residues | |
| CN109111497A (en) | A kind of method for producing of peony seeds albumen | |
| CN105063149B (en) | A kind of method that enzyme process auxiliary prepares cottonseed protein | |
| CN102273578B (en) | Preparation method of rice starch-based fat replacer | |
| CN108034688A (en) | The preparation process of rice bran polysaccharide | |
| CN109170922B (en) | Preparation method of wheat bran soluble dietary fiber | |
| CN109369774A (en) | The extracting method of protein in a kind of acer truncatum seed dregs of rice | |
| CN106916200A (en) | A kind of method that alkali extraction-acid precipitation prepares rubber seed albumen | |
| CN107252117A (en) | A kind of method that oligopeptide, polysaccharide and dietary fiber are produced by raw material of peanut meal | |
| CN110897059A (en) | Method for preparing plant hydrolyzed protein beverage by using wheat germs | |
| CN108244329B (en) | Preparation process of quinoa germ protein powder | |
| CN114164246B (en) | Acer truncatum protein extraction method | |
| CN112079939A (en) | Preparation method of agaricus blazei polysaccharide extract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170704 |