CN108752417A - A kind of pollen pini active peptides - Google Patents
A kind of pollen pini active peptides Download PDFInfo
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- CN108752417A CN108752417A CN201810408279.5A CN201810408279A CN108752417A CN 108752417 A CN108752417 A CN 108752417A CN 201810408279 A CN201810408279 A CN 201810408279A CN 108752417 A CN108752417 A CN 108752417A
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- pollen pini
- active peptides
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Jellies, Jams, And Syrups (AREA)
Abstract
The present invention provides a kind of pollen pini active peptides, belong to technical field of functional food, the preparation method of the pollen pini active peptides of the present invention includes the producing of pollen pini, the preparation of albumen crude extract, the preparation of pollen pini active peptides, raw material pollen pini is cleaned in the forming steps of pollen pini, freezing processing, with crusher in crushing to 5 μm hereinafter, the present invention can effectively improve pollen pini polypeptide yield, and pollen pini healthy nutritive value is promoted, the anti-aging of pollen pini, antioxidant effect are excellent.
Description
Technical field
The invention belongs to technical field of functional food, and in particular to a kind of pollen pini active peptides.
Background technology
Pollen pini is a kind of natural resources of nutrition very abundant, in recent years to aliphatic acid therein and polysaccharose substance into
Numerous studies are gone, but very few to being studied more than the albumen of 10% content.Protein is the base substance in life, and polypeptide is
The decomposition product of protein.Active peptides have reduce blood pressure, slow down aging, is anti-oxidant, liver protection, body beautification beauty treatment, it is antifatigue, change
Kind sleep, enhancing memory, inhibition tumour, enhancing is immune, promotes absorption etc. of the human body to protein and various healthy trace elements with household
Multiple efficacies, but prior art is not high to preserved egg powder polypeptide recovery rate.
Invention content
The purpose of the present invention is to provide one kind effectively improving pollen pini polypeptide yield, and promotes pollen pini nutrition and health care valence
Value, the excellent a kind of pollen pini active peptides of the anti-aging of pollen pini, antioxidant effect.
The technical solution that the present invention is taken to achieve the above object is：A kind of preparation method of pollen pini active peptides,
Producing including pollen pini, the preparation of albumen crude extract, the preparation of pollen pini active peptides, will in the forming steps of pollen pini
Raw material pollen pini cleans, freezing processing, with crusher in crushing to 5 μm hereinafter, stirred using electromagnetic wand, obtains pure pollen pini, passes through
Broken wall treatment is carried out to pollen pini, so that the original fluid behaviour of pollen pini is lost, increases the pollen pini adhesiveness after broken wall, carry significantly
The release of certain nutriments in high pollen pini, improves the health value of pollen pini, the anti-aging that have to pollen pini and anti-
Oxidation effectiveness is obviously improved.
Preferably, freezing processing is that pollen pini is placed at a temperature of -35 DEG C to -28 DEG C to freeze in pollen pini forming steps
4-5h uses magnetic induction to be stirred to obtain pure pollen pini for the electromagnetic wand of 0.015-0.21T after broken, and pollen pini is cold
The percentage of damage that pollen pini cell wall can be effectively improved after jelly processing, is improved, broken wall conducive to total fat, content of starch in pollen pini
The content of peptides in pollen pini crude protein liquid afterwards is effectively promoted simultaneously, and electromagnetic wand is recycled to stir the pollen pini after broken wall
Processing is mixed, the electric current on electromagnetic wand can generate the cell wall of the pollen pini of broken wall and smash effect, especially in cell wall
The space structure of polysaccharide and protein generates change, exposure enzyme cusp position, while hard-packed cell wall can be made loose just
Make the further crushing effect of pollen pini cell wall in the preparation of follow-up pollen pini active peptides, promotes polypeptide yield, realize preserved egg
The promotion of powder effect.
Preferably, the preparation process of albumen crude extract is：The buffer solution for being 7.5-8.5 by pure pollen pini merging pH value
In, solid-liquid ratio 2-4:20, extraction time 2-3h, extracting times are 2-3 times, and extraction temperature is 2-5 DEG C, respectively by each extraction
Liquid centrifugal treating takes supernatant to merge, and obtains albumen crude extract, is preserved egg by carrying out albumen crude extract to pollen pini
The extraction of powder active peptides is prepared.
Preferably, buffer solution is made of following component and parts by weight：50-60 parts of trishydroxymethylaminomethane, 0.1-
60-80 parts of 4-6 parts of 0.15mol/L hydrochloric acid and water, are adjusted the pH value of pollen pini solution by buffer solution, and are conducive to carry
Take preserved egg amyloid proteins crude extract.
Preferably, the preparation process of pollen pini active peptides is placed in the compound protein of 3-5wt% in albumen crude extract
Enzyme stirs and adjusts pH value to 8.0-9.0, the constant temperature standing 2-4h under the conditions of 56-65 DEG C, destroy the enzyme treatment, centrifuging and taking supernatant, thoroughly
Analysis, obtains pollen pini active peptides, using the active peptides in compound protein enzyme r e lease pollen pini, and by optimizing enzymatic hydrolysis condition
Improve hydrolysis result.
Preferably, the sodium ethylene diamine tetracetate containing 0.02-0.05wt% is, it can be achieved that assist compound in compound protease
Protease destroys pollen pini cell wall, accelerates cell wall hydrolysis to accelerate cell wall and intracellular structure to disintegrate, breaks cell wall
Inhibition, so that the nutriment in pollen pini is released, can also reduce enzymolysis fluid viscosity, change the infiltration of enzymolysis liquid
Pressure shortens enzymolysis time, improves hydrolysis result, realizes that the yield of the polypeptide of pollen pini improves, the promotion of nutrient content.
Preferably, compound protease is 1-1.5 by mass ratio:2 alkali protease and neutral proteinase composition, is selected
Compound protease can effectively promote hydrolysis result, using the specificity of enzyme, avoid being acted on by inscribe enzyme specificity in enzymolysis process
The limitation in site, hydrolysis is insufficient to lead to the reduction of pollen pini polypeptide yield.
Preferably, in dialysis procedure, molecular cut off 5000-10000Da can remove in obtained polypeptide solution
Salt, organic solvent, biological micromolecule impurity etc. improve the quality and effect of polypeptide.
Compared with prior art, beneficial effects of the present invention are：The present invention makes pine by carrying out broken wall treatment to pollen pini
The original fluid behaviour of pollen is lost, and increases the pollen pini adhesiveness after broken wall, greatly improves certain nutriments in pollen pini
Release, improves the health value of pollen pini, the anti-aging and antioxidant effect have to pollen pini is obviously improved.By this hair
Bright extraction process can effectively improve pollen pini polypeptide yield, and promote pollen pini healthy nutritive value, and pollen pini delays to decline
Always, antioxidant effect is excellent.
The present invention provides a kind of pollen pini active peptides using above-mentioned technical proposal, compensates for the deficiencies in the prior art, grasps
Make simple, it is easy to accomplish.
Specific implementation mode
Technical scheme of the present invention is described in further detail with reference to embodiments：
Embodiment 1：
A kind of preparation method of pollen pini active peptides, including the producing of pollen pini, the preparation of albumen crude extract, pollen pini are lived
The preparation of property polypeptide, concrete operations are as follows：
1）Raw material pollen pini is cleaned in the forming steps of pollen pini, 4h is freezed at a temperature of pollen pini is placed in -32 DEG C, with broken
Crusher machine to 5 μm hereinafter, use magnetic induction for 0.017T electromagnetic wand stir, obtain pure pollen pini；
2）The preparation of albumen crude extract：In the buffer solution for being 7.7 by pure pollen pini merging pH value, solid-liquid ratio 3:20, extraction
Time is 2h, and extracting times are 2 times, and extraction temperature is 3 DEG C, takes supernatant to merge each leaching liquor centrifugal treating respectively,
Buffer solution is made of following component and parts by weight：72 parts of 54 parts of trishydroxymethylaminomethane, 5 parts of 0.12mol/L hydrochloric acid and water；
3）The preparation of pollen pini active peptides：The compound protease of 4wt% is placed in albumen crude extract, in compound protease
Sodium ethylene diamine tetracetate containing 0.03wt% stirs and adjusts pH value to 8.4, the constant temperature standing 3h under the conditions of 58 DEG C, at enzyme deactivation
Reason, centrifuging and taking supernatant are dialysed, and in dialysis procedure, molecular cut off 6000Da obtains pollen pini active peptides；
Compound protease is 1.2 by mass ratio:2 alkali protease and neutral proteinase composition.
Embodiment 2：
A kind of preparation method of pollen pini active peptides, including the producing of pollen pini, the preparation of albumen crude extract, pollen pini are lived
The preparation of property polypeptide, concrete operations are as follows：
1）Raw material pollen pini is cleaned in the forming steps of pollen pini, 5h is freezed at a temperature of pollen pini is placed in -30 DEG C, with broken
Crusher machine to 5 μm hereinafter, use magnetic induction for 0.015T electromagnetic wand stir, obtain pure pollen pini；
2）The preparation of albumen crude extract：In the buffer solution for being 8.0 by pure pollen pini merging pH value, solid-liquid ratio 1:10, extraction
Time is 2h, and extracting times are 3 times, and extraction temperature is 5 DEG C, takes supernatant to merge each leaching liquor centrifugal treating respectively,
Buffer solution is made of following component and parts by weight：74 parts of 55 parts of trishydroxymethylaminomethane, 5 parts of 0.14mol/L hydrochloric acid and water；
3）The preparation of pollen pini active peptides：The compound protease of 3wt% is placed in albumen crude extract, in compound protease
Sodium ethylene diamine tetracetate containing 0.03wt% stirs and adjusts pH value to 8.6, the constant temperature standing 3h under the conditions of 59 DEG C, at enzyme deactivation
Reason, centrifuging and taking supernatant are dialysed, and in dialysis procedure, molecular cut off 8000Da obtains pollen pini active peptides；
Compound protease is 1.1 by mass ratio:2 alkali protease and neutral proteinase composition.
Embodiment 3：
A kind of preparation method of pollen pini active peptides, including the producing of pollen pini, the preparation of albumen crude extract, pollen pini are lived
The preparation of property polypeptide, concrete operations are as follows：
1）Raw material pollen pini is cleaned in the forming steps of pollen pini, 5h is freezed at a temperature of pollen pini is placed in -29 DEG C, with broken
Crusher machine to 5 μm hereinafter, use magnetic induction for 0.21T electromagnetic wand stir, obtain pure pollen pini；
2）The preparation of albumen crude extract：In the buffer solution for being 8.4 by pure pollen pini merging pH value, solid-liquid ratio 1:5, when extraction
Between be 3h, extracting times be 3 times, extraction temperature be 3 DEG C, take supernatant to merge each leaching liquor centrifugal treating respectively, delay
Fliud flushing is made of following component and parts by weight：74 parts of 54 parts of trishydroxymethylaminomethane, 5 parts of 0.12mol/L hydrochloric acid and water；
3）The preparation of pollen pini active peptides：The compound protease of 4wt% is placed in albumen crude extract, in compound protease
Sodium ethylene diamine tetracetate containing 0.04wt% stirs and adjusts pH value to 8.4, the constant temperature standing 3h under the conditions of 62 DEG C, at enzyme deactivation
Reason, centrifuging and taking supernatant are dialysed, and in dialysis procedure, molecular cut off 7000Da obtains pollen pini active peptides；
Compound protease is 1.3 by mass ratio:2 alkali protease and neutral proteinase composition.
Pharmacological testing：
Anti-aging is tested：The pollen pini active peptides that embodiment 2 is prepared continuously are taken one month to the small white mouse of health
（Experimental group）, its liver, brain and the heart are removed, SOD in liver is measured（Superoxide dismutase）Activity（Such as table 1）And brain, the heart
With the amount of lipofuscin in liver（Such as table 2）, and with the small white mouse of the pollen pini active peptides to take the embodiment of the present invention 2（It is right
According to group）It is compared, situation is as follows：
Table 1
| Group | Pollen pini active peptides dosage（mg/kg） | Small white mouse quantity（Only） | SOD（μg/mg） |
| Control group | 0 | 10 | 1.51±0.26 |
| Experimental group 1 | 300 | 10 | 2.24±0.32 |
| Experimental group 2 | 600 | 10 | 2.53±0.38 |
Table 2
Activity by the SOD in the visible Liver of Mice taken after the pollen pini active peptides that extract of the present invention of table 1 is apparent high
In control group, and the activity of the more SOD of dose is higher, it is seen that the anti-aging effects for the pollen pini active peptides that the present invention extracts
It is excellent；Pollen pini active peptides hindbrain, liver, the lipofuscin in the heart that the present invention is taken by 2 visible small white mouse of table are substantially reduced.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme of the present invention is described in detail in embodiment described above, it should be understood that the above is only
For specific embodiments of the present invention, it is not intended to restrict the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should all be included in the protection scope of the present invention.
Claims (9)
1. a kind of preparation method of pollen pini active peptides, including the producing of pollen pini, the preparation of albumen crude extract, pollen pini
The preparation of active peptides, it is characterised in that：Raw material pollen pini is cleaned in the forming steps of pollen pini, freezing processing, with broken
Crusher machine obtains pure pollen pini to 5 μm hereinafter, stirred using electromagnetic wand.
2. wanting a kind of preparation method of pollen pini active peptides described in 1 according to right, it is characterised in that：The system of the pollen pini
It is that pollen pini is placed at a temperature of -35 DEG C to -28 DEG C to freeze 4-5h to take in step freezing processing, used after being crushed magnetic induction for
The electromagnetic wand of 0.015-0.21T is stirred to obtain pure pollen pini.
3. wanting a kind of preparation method of pollen pini active peptides described in 1 according to right, it is characterised in that：The albumen coarse extraction
The preparation process of liquid is：In the buffer solution for being 7.5-8.5 by pure pollen pini merging pH value, solid-liquid ratio 2-4:20, extraction time
For 2-3h, extracting times are 2-3 times, and extraction temperature is 2-5 DEG C, takes supernatant to close each leaching liquor centrifugal treating respectively
And obtain albumen crude extract.
4. wanting a kind of preparation method of pollen pini active peptides described in 3 according to right, it is characterised in that：The buffer solution by with
Lower ingredient and parts by weight composition：60-80 parts of 50-60 parts of trishydroxymethylaminomethane, 4-6 parts of 0.1-0.15mol/L hydrochloric acid and water.
5. wanting a kind of preparation method of pollen pini active peptides described in 1 according to right, it is characterised in that：The pollen pini activity
The preparation process of polypeptide is placed in the compound protease of 3-5wt% in albumen crude extract, stirs and adjusts pH value to 8.0-9.0, in
Constant temperature stands 2-4h, destroy the enzyme treatment under the conditions of 56-65 DEG C, and centrifuging and taking supernatant dialyses, obtains pollen pini active peptides.
6. wanting a kind of preparation method of pollen pini active peptides described in 5 according to right, it is characterised in that：The compound protease
In the sodium ethylene diamine tetracetate containing 0.02-0.05wt%.
7. wanting a kind of preparation method of pollen pini active peptides described in 5 according to right, it is characterised in that：The compound protease
It is 1-1.5 by mass ratio:2 alkali protease and neutral proteinase composition.
8. wanting a kind of preparation method of pollen pini active peptides described in 5 according to right, it is characterised in that：The dialysis procedure
In, molecular cut off 5000-10000Da.
9. a kind of pollen pini active peptides prepare gained according to claim 1-8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810408279.5A CN108752417A (en) | 2018-05-02 | 2018-05-02 | A kind of pollen pini active peptides |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810408279.5A CN108752417A (en) | 2018-05-02 | 2018-05-02 | A kind of pollen pini active peptides |
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| Publication Number | Publication Date |
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| CN108752417A true CN108752417A (en) | 2018-11-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810408279.5A Withdrawn CN108752417A (en) | 2018-05-02 | 2018-05-02 | A kind of pollen pini active peptides |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111621538A (en) * | 2020-06-22 | 2020-09-04 | 浙江亚林生物科技股份有限公司 | Preparation method and application of pollen pini oligopeptide |
| CN114081854A (en) * | 2021-11-19 | 2022-02-25 | 烟台新时代健康产业日化有限公司 | Pollen pini extract with barrier repair function and fermentation preparation process thereof |
-
2018
- 2018-05-02 CN CN201810408279.5A patent/CN108752417A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111621538A (en) * | 2020-06-22 | 2020-09-04 | 浙江亚林生物科技股份有限公司 | Preparation method and application of pollen pini oligopeptide |
| CN114081854A (en) * | 2021-11-19 | 2022-02-25 | 烟台新时代健康产业日化有限公司 | Pollen pini extract with barrier repair function and fermentation preparation process thereof |
| CN114081854B (en) * | 2021-11-19 | 2023-08-15 | 烟台新时代健康产业日化有限公司 | Pollen pini extract with barrier repairing function and fermentation preparation process thereof |
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