CN103789382A - Method for rapidly extracting fish collagen protein by using enzymolysis method - Google Patents
Method for rapidly extracting fish collagen protein by using enzymolysis method Download PDFInfo
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Abstract
The invention discloses a method for rapidly extracting fish collagen protein by using an enzymolysis method. Degreasing treatment and protein extraction are accomplished in one step by using a high pressure ultrasonic enzymolysis agent, the extraction efficiency is greatly improved, and the protection time is shortened. The extraction process is environment-friendly, rapid and convenient, uniform in fish collagen protein molecular weight distribution, and small in impurity.
Description
Technical field
The invention belongs to biological products preparing technical field, particularly a kind of method of enzymolysis process rapid extraction Isin glue collagen.
Background technology
Collagen protein is a kind ofly in reticular tissue to have the important structure albumen that enriches physiological function, unique macromole spirane structure and aggregated structure that it has, make it have certain thermostability and very high mechanical tenacity and intensity, given its unique distinction for food, pharmaceuticals, wrapping material.Collagen protein has very strong hydrophilic, performance of keeping humidity, it can be used as additive in food, with improve food local flavor, improve preservation stationary phase of food, can be used as the jelling agent, stablizer, emulsifying agent, thickening material, whipping agent, tackiness agent, finings of food etc.Collagen protein is as natural Biological resources, and the application in the high added value industry such as food, medicine, makeup, dietary supplements is at present more and more extensive, is more and more subject to people's attention.The function of the peculiar extremely low antigenicity of collagen protein, high biodegradable, bioresorbable, hemostatic function and Promote cell's growth was also widely used in recent years in medicine industry, clinical medicine and clinical treatment.According to the difference of clinical requirement, collagen is carried out to corresponding processing treatment, can be prepared into collagen injection liquid, collagen hemostasis pulvis, stanch fibre, styptic sponge, operation oversewing material, heart valve repair material, surgical dressing, dermatoplasty material, and with equivalent material of blood vessel, oesophagus and the tracheae of tubular form etc.These materials are widely used in reparation, shaping and the beauty treatment of wound and burn, and skin and nerve growth, and blood vessel, valve surgery etc., show good application prospect.
Traditional collagen protein extracting method is skin and the bone from pig, ox, and extracts in bird tissue.But in recent years because of the outburst of the diseases such as mad cow disease, foot and mouth disease, bird flu, make people produce doubt to livestock and bird collagen products safety.In addition, due to the reason such as religion and custom, extract collagen protein and be very limited from pigskin ox-hide, therefore, the new source of finding collagen protein seems more and more urgent.Extract the collagen protein safe, specific absorption is high, biological activity is stronger from seawater or fresh-water fishes body waste and will become the main source that collagen product extracts.
The removal problem of existing Isin glue collagen extracting method fat in often having ignored fish body waste while processing early stage, makes fat sneak into the purity that affects collagen protein in collagen protein, and has restricted its application in field, downstream.In addition, fatty removal methods generally adopts acid, alkaline solution or organic solvent method, and these methods not only cost are higher, and degreasing is not thorough, and the use of chemical reagent easily makes collagen protein sex change, reduction product yield.Traditional collagen protein extracting method adopts acid or alkaline degradation collagen protein to extract, and these methods can cause partial amino-acid to destroy in degradation process, and Product Safety is poor, biological activity loss, and the waste liquid in production is to environment; Adopt low-temperature biological zymolysis technique, do not add other materials, can improve product quality (as CN102533914 A), avoid environmental pollution, but required time is longer, molecular weight product is lower, and the collagen molecules amount skewness obtaining.
In order to accelerate the production cycle of Isin glue collagen, reduce production costs, preparation can meet the Isin glue collagen of different molecular weight needs.This patent adopts the ultrasonic enzymolysis skill of high pressure, and a step completes skimming treatment and protein extraction, has greatly improved extraction efficiency, has shortened the production time.Leaching process environmental protection, quick and convenient, Isin glue collagen even molecular weight distribution, impurity is few, can be used as starting material and is applied to dietary supplements, makeup, food and medical field.
Summary of the invention
The object of this invention is to provide a kind of method of enzymolysis process rapid extraction Isin glue collagen.Preparation process of the present invention is simple, easy to operate, cost is low, the Isin glue collagen even molecular weight distribution of preparation, and impurity is few, can make to meet dietary supplements, makeup, food and the demand of Application Areas to different molecular weight Isin glue collagen such as medical.
A kind of method that discloses enzymolysis process rapid extraction Isin glue collagen of the present invention, comprises the steps:
(1) clean fish body waste is cut into 50～200 object fish meal;
(2) in encloses container, add fish meal, then add distilled water, reaction enzymes stirring to make slurries, it is 50～70 ℃ that slurries are controlled temperature of reaction under high pressure, supersound process, and pH is 7-9, stirs 0.5～2h;
(3) in the slurries that obtain, add a small amount of sodium hydroxide after step (2) reaction, and be heated to 80～90 ℃, stir 20～30min;
(4) filter slurries, filtrate obtains transparent Isin glue collagen solution through dialysis.
As preferred technical scheme:
Wherein, the method for a kind of enzymolysis process rapid extraction Isin glue collagen as above, the distilled water quality described in step (2) is 3～10 times of described quality of fishmeal.
The method of a kind of enzymolysis process rapid extraction Isin glue collagen as above, described reaction enzymes refers to the mixture of lipase and proteolytic enzyme; Wherein lipase refers to neutral lipase and/or alkaline lipase, and proteolytic enzyme refers to bromeline, Sumizyme MP and/or trypsinase.In this patent, enzymolysis degreasing and enzymolysis and extraction are synchronously carried out: enzymolysis degreasing is the enzymolysis that utilizes lipase, and at suitable temperature and stir speed (S.S.), lipase can become glycerine and lipid acid by the ester linkage hydrolyzing of triglyceride level rapidly; Enzymolysis and extraction is to utilize the enzymolysis of proteolytic enzyme, the peptide bond hydrolysis in catalytic proteins, thus obtain Isin glue collagen solution.
Further, described reaction enzymes adds quality to account for 0.1～1% of quality of fishmeal, and wherein the mass ratio of lipase and proteolytic enzyme is 1:2～4.Because enzymolysis degreasing and enzymolysis and extraction in this patent are synchronously carried out, in order to make enzymolysis degreasing match and not interfere with each other with the speed of enzymolysis and extraction, must strict usage quantity and the usage ratio of controlling lipase and proteolytic enzyme.
The method of a kind of enzymolysis process rapid extraction Isin glue collagen as above, described high pressure refers to that the pressure of controlling in encloses container is at 1.5～3 standard atmospheric pressures; Described supersound process refers to and adopts ultrasonic generator to make material in the encloses container supersound process in 15～25kHz.High pressure and supersound process can effectively improve the catalytic rate of proteolytic enzyme, but in supersound process, ultrasonic frequency and time need to control, to avoid excessive handling to make protein denaturation.
The method of a kind of enzymolysis process rapid extraction Isin glue collagen as above, described addition amount of sodium hydroxide accounts for the 0.1-0.5% of step (2) reaction rear slurry quality.The object that sodium hydroxide adds is that adjusting enzyme digestion reaction rear slurry system is weakly alkaline, so that the fatty acid response that fatty enzymolysis generates generates soap salt.
The method of a kind of enzymolysis process rapid extraction Isin glue collagen as above, described dialysis refers to filtrate is placed in to dialysis tubing through distill water dialysis 4-7 days, wherein said dialysis tubing is that molecular weight cut-off is 1-300KD.Adopt dialysis treatment can remove original solubility small molecules foreign material in filtrate, and control the Isin glue collagen obtaining in required molecular weight ranges.
The method of a kind of enzymolysis process rapid extraction Isin glue collagen as above, described fish body waste refers to fish-skin, fish scale and/or air bladder.In this patent, fish body waste is processed into tiny particle, can significantly improve like this efficiency of enzymolysis, improve the yield of final Isin glue collagen.
Applicant finds unexpectedly, alkaline lipase: bromeline mass ratio is 1:3, accounts for fish meal weight 0.7%, and enzymolysis pH value is set as at 8 o'clock, and extraction effect is best, and extraction yield is the highest
Compared with prior art, the invention has the beneficial effects as follows:
This patent adopts the ultrasonic enzymolysis agent of high pressure, and a step completes skimming treatment and protein extraction, has greatly improved extraction efficiency, has shortened the production time.Leaching process environmental protection, quick and convenient, Isin glue collagen even molecular weight distribution, impurity is few, can make to meet dietary supplements, makeup, food and the demand of Application Areas to different molecular weight Isin glue collagen such as medical.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment are only not used in and limit the scope of the invention for the present invention is described.In addition should be understood that those skilled in the art can make various changes or modifications the present invention after having read the content of the present invention's instruction, these equivalent form of values fall within the application's appended claims limited range equally.
Embodiment 1:
A method for enzymolysis process rapid extraction Isin glue collagen, comprises the steps:
(1) cut into fritter by removing surface impurity clean fish-skin, fish scale, air bladder, and be cut into 50 object fish meal with crusher;
(2) in encloses container, add fish meal, being incorporated as quality of fishmeal again and being the distilled water of 3 times and reaction enzymes stirs and makes slurries, wherein reaction enzymes is made up of neutral lipase and bromeline, reaction enzymes add-on accounts for 0.1% of quality of fishmeal, and the mass ratio 1:4 of neutral lipase and bromeline, it is 50 ℃ that the slurries that prepare are controlled temperature of reaction under the supersound process of 1.5 standard atmospheric pressures, 15kHz, and pH is 7, stirs 2h and carries out enzyme digestion reaction;
(3) in the slurries that obtain, add the sodium hydroxide that accounts for its quality 0.1% after step (2) reaction, and be heated to 90 ℃, stir 20min;
(4) filter slurries, it is that the dialysis tubing of 1KD obtains transparent Isin glue collagen solution for 7 days through distill water dialysis that filtrate is placed in to molecular weight cut-off.
Embodiment 2:
A method for enzymolysis process rapid extraction Isin glue collagen, comprises the steps:
(1) cut into fritter by removing surface impurity clean fish-skin, fish scale, air bladder, and be cut into 100 object fish meal with crusher;
(2) in encloses container, add fish meal, add again that to account for quality of fishmeal be that the distilled water of 5 times and reaction enzymes stir and make slurries, wherein reaction enzymes is made up of neutral lipase and Sumizyme MP, reaction enzymes add-on accounts for 0.3% of quality of fishmeal, and the mass ratio 1:4 of neutral lipase and Sumizyme MP, it is 55 ℃ that the slurries that prepare are controlled temperature of reaction under the supersound process of 2 standard atmospheric pressures, 20kHz, and pH is 7.5, stirs 1.5h and carries out enzyme digestion reaction;
(3) in the slurries that obtain, add the sodium hydroxide that accounts for its quality 0.2% after step (2) reaction, and be heated to 80 ℃, stir 30min;
(4) filter slurries, it is that the dialysis tubing of 2KD obtains transparent Isin glue collagen solution for 4 days through distill water dialysis that filtrate is placed in to molecular weight cut-off.
Embodiment 3:
A method for enzymolysis process rapid extraction Isin glue collagen, comprises the steps:
(1) cut into fritter by removing surface impurity clean fish-skin, fish scale, air bladder, and be cut into 150 object fish meal with crusher;
(2) in encloses container, add fish meal, add again that to account for quality of fishmeal be that the distilled water of 7 times and reaction enzymes stir and make slurries, wherein reaction enzymes is made up of neutral lipase and trypsinase, reaction enzymes add-on accounts for 0.5% of quality of fishmeal, and neutral lipase and tryptic mass ratio 1:3, it is 70 ℃ that the slurries that prepare are controlled temperature of reaction under the supersound process of 2.5 standard atmospheric pressures, 25kHz, and pH is 7.5, stirs 1h and carries out enzyme digestion reaction;
(3) in the slurries that obtain, add the sodium hydroxide that accounts for its quality 0.3% after step (2) reaction, and be heated to 90 ℃, stir 30min;
(4) filter slurries, it is that the dialysis tubing of 5KD obtains transparent Isin glue collagen solution for 5 days through distill water dialysis that filtrate is placed in to molecular weight cut-off.
Embodiment 4:
A method for enzymolysis process rapid extraction Isin glue collagen, comprises the steps:
(1) cut into fritter by removing surface impurity clean fish-skin, fish scale, air bladder, and be cut into 200 object fish meal with crusher;
(2) in encloses container, add fish meal, add again that to account for quality of fishmeal be that the distilled water of 9 times and reaction enzymes stir and make slurries, wherein reaction enzymes is made up of alkaline lipase and bromeline, reaction enzymes add-on accounts for 0.7% of quality of fishmeal, and the mass ratio 1:3 of alkaline lipase and bromeline, it is 65 ℃ that the slurries that prepare are controlled temperature of reaction under the supersound process of 3 standard atmospheric pressures, 15kHz, and pH is 8, stirs 0.5h and carries out enzyme digestion reaction;
(3) in the slurries that obtain, add the sodium hydroxide that accounts for its quality 0.4% after step (2) reaction, and be heated to 80 ℃, stir 20min;
(4) filter slurries, it is that the dialysis tubing of 10KD obtains transparent Isin glue collagen solution for 6 days through distill water dialysis that filtrate is placed in to molecular weight cut-off.
Embodiment 5:
A method for enzymolysis process rapid extraction Isin glue collagen, comprises the steps:
(1) cut into fritter by removing surface impurity clean fish-skin, fish scale, air bladder, and be cut into 50 object fish meal with crusher;
(2) in encloses container, add fish meal, add again that to account for quality of fishmeal be that the distilled water of 10 times and reaction enzymes stir and make slurries, wherein reaction enzymes is made up of alkaline lipase and Sumizyme MP, reaction enzymes add-on accounts for 0.9% of quality of fishmeal, and the mass ratio 1:2 of alkaline lipase and Sumizyme MP, it is 60 ℃ that the slurries that prepare are controlled temperature of reaction under the supersound process of 2 standard atmospheric pressures, 20kHz, and pH is 8.5, stirs 1h and carries out enzyme digestion reaction;
(3) in the slurries that obtain, add the sodium hydroxide that accounts for its quality 0.5% after step (2) reaction, and be heated to 90 ℃, stir 30min;
(4) filter slurries, it is that the dialysis tubing of 100KD obtains transparent Isin glue collagen solution for 7 days through distill water dialysis that filtrate is placed in to molecular weight cut-off.
Embodiment 6:
A method for enzymolysis process rapid extraction Isin glue collagen, comprises the steps:
(1) cut into fritter by removing surface impurity clean fish-skin, fish scale, air bladder, and be cut into 100 object fish meal with crusher;
(2) in encloses container, add fish meal, add again that to account for quality of fishmeal be that the distilled water of 7 times and reaction enzymes stir and make slurries, wherein reaction enzymes is by alkaline lipase: bromeline=1:3 forms, reaction enzymes add-on accounts for 0.7% of quality of fishmeal, it is 55 ℃ that the slurries that prepare are controlled temperature of reaction under the supersound process of 1.5 standard atmospheric pressures, 15kHz, pH is 8, stirs 0.5h and carries out enzyme digestion reaction;
(3) in the slurries that obtain, add the sodium hydroxide that accounts for its quality 0.1% after step (2) reaction, and be heated to 90 ℃, stir 30min;
(4) filter slurries, it is that the dialysis tubing of 300KD obtains transparent Isin glue collagen solution for 7 days through distill water dialysis that filtrate is placed in to molecular weight cut-off.
Embodiment 7:
Experiment condition screening:
(1) cut into fritter by removing surface impurity clean fish-skin, fish scale, air bladder, and be cut into 200 object fish meal with crusher;
(2) in encloses container, add fish meal, add again that to account for quality of fishmeal be that the distilled water of 9 times and reaction enzymes stir and make slurries, reaction enzymes add-on accounts for 0.7% of quality of fishmeal, it is 65 ℃ that the slurries that prepare are controlled temperature of reaction under the supersound process of 3 standard atmospheric pressures, 15kHz, regulate pH value, stir 0.5h and carry out enzyme digestion reaction;
(3) in the slurries that obtain, add the sodium hydroxide that accounts for its quality 0.4% after step (2) reaction, and be heated to 80 ℃, stir 70min;
(4) filter slurries, it is that the dialysis tubing of 100KD obtains transparent Isin glue collagen solution for 7 days through distill water dialysis that filtrate is placed in to molecular weight cut-off.
When concrete operations, take in the composition kind of reaction enzymes and enzymolysis pH one and calculate extraction yield by following formula as variable (referring to table one, two, three), determine optimum experimental condition.
Collagen content is measured (in hydroxyproline content) and purity, extraction yield calculates, according to IS03496:1978(E) method, measure hydroxyproline content.Collagen content equals hydroxyproline content and is multiplied by coefficient 11.1.
Collagen purity=11.1m1/m2 × 100%
Wherein, the oxyproline quality (g) of m1---mensuration, m2---the collagen quality (g) taking while measuring oxyproline.
Collagen protein extraction yield=(collagen purity × M1/M2) 100%
Wherein, the quality (g) of M1---finished product collagen, the quality (g) of M2---fish body waste.
Table one changes the composition of reaction enzymes
| Numbering | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 8 |
| PH value | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 |
| Composition | Neutral lipase: bromeline | Neutral lipase: Sumizyme MP | Neutral lipase: trypsinase | Alkaline lipase: bromeline | Alkaline lipase: Sumizyme MP | Alkaline lipase: trypsinase | Alkaline lipase: bromeline | Alkaline lipase: bromeline | Neutral lipase: bromeline |
| Proportioning | 1:4 | 1:4 | 1:3 | 1:3 | 1:2 | 1:2 | 1:2 | 1:4 | 1:3 |
| Extraction yield | 29.1% | 29.3% | 29.2% | 29.7% | 28.9% | 29.1% | 29.1% | 29.3% | 29.2% |
Table two reaction enzymes accounts for the massfraction of fish meal
| Numbering | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
| PH value | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 |
| Reaction enzymes | Alkaline lipase: bromeline=1:3 | Alkaline lipase: bromeline=1:3 | Alkaline lipase: bromeline=1:3 | Alkaline lipase: bromeline=1:3 | Alkaline lipase: bromeline=1:3 | Alkaline lipase: bromeline=1:3 | Alkaline lipase: bromeline=1:3 | Alkaline lipase: bromeline=1:3 | Alkaline lipase: bromeline=1:3 |
| Reaction fish meal slurry weight | 0.1% | 0.2% | 0.4% | 0.5% | 0.6% | 0.7% | 0.8% | 0.8% | 1.0% |
| Extraction yield | 29.8% | 29.9% | 30.2% | 30.3% | 30.5% | 31.0% | 30.7% | 30.3% | 30.0% |
Table three changes the pH value of enzymolysis
| Numbering | 1 | 2 | 3 | 4 | 6 |
| PH value | 7 | 7.5 | 8 | 8.5 | 9 |
| Reaction enzymes | Alkaline lipase: bromeline=1:3 | Alkaline lipase: bromeline=1:3 | Alkaline lipase: bromeline=1:3 | Alkaline lipase: bromeline=1:3 | Alkaline lipase: bromeline=1:3 |
| Reaction fish meal slurry weight | 0.7% | 0.7% | 0.7% | 0.7% | 0.7% |
| Extraction yield | 32.0% | 32.5% | 33.1% | 32.6% | 32.4% |
From the above, as selected alkaline lipase: the bromeline=1:3 of reaction enzymes, account for fish meal weight 0.7%, enzymolysis pH value is set as at 8 o'clock, and extraction effect is best, and extraction yield is the highest.Under these conditions, extract that the collagen product color and luster that obtains is pure white, free from extraneous odour.
Finally it should be noted that: the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although the present invention is had been described in detail with reference to previous embodiment, for a person skilled in the art, its technical scheme that still can record aforementioned each embodiment is modified, or part technical characterictic is wherein equal to replacement.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (8)
1. a method for enzymolysis process rapid extraction Isin glue collagen, is characterized in that: comprises the steps,
Clean fish body waste is cut into into 50～200 object fish meal;
In encloses container, add fish meal, then add distilled water, reaction enzymes stirring to make slurries, it is 50～70 ℃ that slurries are controlled temperature of reaction under high pressure, supersound process, and pH is 7-9, stirs 0.5～2h;
In the slurries that obtain, add a small amount of sodium hydroxide after step (2) reaction, and be heated to 80～90 ℃, stir 20～30min;
Filter slurries, filtrate obtains transparent Isin glue collagen solution through dialysis.
2. the method for claim 1, is characterized in that: the distilled water quality described in step (2) is 3～10 times of described quality of fishmeal.
3. method as claimed in claim 1 or 2, is characterized in that: described reaction enzymes refers to the mixture of lipase and proteolytic enzyme; Wherein lipase refers to neutral lipase and/or alkaline lipase, and proteolytic enzyme refers to bromeline, Sumizyme MP and/or trypsinase.
4. method as claimed in claim 3, is characterized in that: described reaction enzymes adds quality to account for 0.1～1% of quality of fishmeal, and wherein the mass ratio of lipase and proteolytic enzyme is 1:2～4.
5. method as claimed in claim 1 or 2, is characterized in that: described high pressure refers to that the pressure of controlling in encloses container is at 1.5～3 standard atmospheric pressures; Described supersound process refers to and adopts ultrasonic generator to make material in the encloses container supersound process in 15～25kHz.
6. method as claimed in claim 1 or 2, is characterized in that: described addition amount of sodium hydroxide accounts for the 0.1-0.5% of step (2) reaction rear slurry quality.
7. method as claimed in claim 1 or 2, is characterized in that: described dialysis refers to filtrate is placed in to dialysis tubing through distill water dialysis 4-7 days, and wherein said dialysis tubing is that molecular weight cut-off is 1-300KD.
8. method as claimed in claim 1 or 2, is characterized in that: described fish body waste refers to fish-skin, fish scale and/or air bladder.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104788559A (en) * | 2015-04-28 | 2015-07-22 | 黑龙江力海鑫生物科技股份有限公司 | Biomedical mouse tail collagen extraction method |
| CN105753971A (en) * | 2016-04-11 | 2016-07-13 | 马鞍山中粮生物化学有限公司 | Collagen extraction method |
| CN107653288A (en) * | 2017-10-13 | 2018-02-02 | 金华市川璞农业科技有限公司 | With the purposes of catfish fish guts protease extraction collagen |
| CN108977424A (en) * | 2018-08-02 | 2018-12-11 | 山东隆科特酶制剂有限公司 | A kind of catabolic enzyme preparation of the molten slurry of anchovy fish and its application |
| CN116694234A (en) * | 2023-06-09 | 2023-09-05 | 四川瑞宝生物科技股份有限公司 | Method for rapidly preparing bone gelatin |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103386144A (en) * | 2013-07-23 | 2013-11-13 | 海南光宇生物科技有限公司 | Preparation method of fish collagen combined chitosan biological dressing |
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2014
- 2014-02-14 CN CN201410050681.2A patent/CN103789382A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103386144A (en) * | 2013-07-23 | 2013-11-13 | 海南光宇生物科技有限公司 | Preparation method of fish collagen combined chitosan biological dressing |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104788559A (en) * | 2015-04-28 | 2015-07-22 | 黑龙江力海鑫生物科技股份有限公司 | Biomedical mouse tail collagen extraction method |
| CN104788559B (en) * | 2015-04-28 | 2018-04-13 | 黑龙江力海鑫生物科技股份有限公司 | A kind of extracting method of bio-medical rat tail collagen protein |
| CN105753971A (en) * | 2016-04-11 | 2016-07-13 | 马鞍山中粮生物化学有限公司 | Collagen extraction method |
| CN107653288A (en) * | 2017-10-13 | 2018-02-02 | 金华市川璞农业科技有限公司 | With the purposes of catfish fish guts protease extraction collagen |
| CN108977424A (en) * | 2018-08-02 | 2018-12-11 | 山东隆科特酶制剂有限公司 | A kind of catabolic enzyme preparation of the molten slurry of anchovy fish and its application |
| CN116694234A (en) * | 2023-06-09 | 2023-09-05 | 四川瑞宝生物科技股份有限公司 | Method for rapidly preparing bone gelatin |
| CN116694234B (en) * | 2023-06-09 | 2024-04-12 | 四川瑞宝生物科技股份有限公司 | Method for rapidly preparing bone gelatin |
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Application publication date: 20140514 |