CN101538602A - Extraction method of fish skin collagen - Google Patents
Extraction method of fish skin collagen Download PDFInfo
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- CN101538602A CN101538602A CN200910131265A CN200910131265A CN101538602A CN 101538602 A CN101538602 A CN 101538602A CN 200910131265 A CN200910131265 A CN 200910131265A CN 200910131265 A CN200910131265 A CN 200910131265A CN 101538602 A CN101538602 A CN 101538602A
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- fish
- skin
- collagen
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- enzymolysis
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- 102000008186 Collagen Human genes 0.000 title claims abstract description 36
- 108010035532 Collagen Proteins 0.000 title claims abstract description 36
- 229920001436 collagen Polymers 0.000 title claims abstract description 26
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 23
- 238000000605 extraction Methods 0.000 title abstract description 3
- 102000004190 Enzymes Human genes 0.000 claims abstract description 13
- 108090000790 Enzymes Proteins 0.000 claims abstract description 13
- 102000004882 Lipase Human genes 0.000 claims abstract description 6
- 108090001060 Lipase Proteins 0.000 claims abstract description 6
- 238000005238 degreasing Methods 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000002994 raw material Substances 0.000 claims abstract description 5
- 239000004367 Lipase Substances 0.000 claims abstract description 4
- 235000019421 lipase Nutrition 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 13
- 239000006228 supernatant Substances 0.000 claims description 9
- 108091005804 Peptidases Proteins 0.000 claims description 6
- 239000000047 product Substances 0.000 claims description 6
- 238000010792 warming Methods 0.000 claims description 6
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims description 5
- 229910001861 calcium hydroxide Inorganic materials 0.000 claims description 5
- 239000000920 calcium hydroxide Substances 0.000 claims description 5
- 239000012535 impurity Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 239000000284 extract Substances 0.000 claims description 3
- JEIPFZHSYJVQDO-UHFFFAOYSA-N ferric oxide Chemical compound O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 claims description 3
- 235000013305 food Nutrition 0.000 claims description 3
- 102000035195 Peptidases Human genes 0.000 claims description 2
- 239000003292 glue Substances 0.000 claims 2
- 239000000654 additive Substances 0.000 claims 1
- 230000000996 additive effect Effects 0.000 claims 1
- 239000012141 concentrate Substances 0.000 claims 1
- 239000000843 powder Substances 0.000 claims 1
- 238000001035 drying Methods 0.000 abstract description 5
- 238000004140 cleaning Methods 0.000 abstract 1
- 238000005202 decontamination Methods 0.000 abstract 1
- 230000003588 decontaminative effect Effects 0.000 abstract 1
- 238000001976 enzyme digestion Methods 0.000 abstract 1
- 239000000706 filtrate Substances 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 description 11
- 239000004365 Protease Substances 0.000 description 6
- 239000003513 alkali Substances 0.000 description 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 4
- 239000003960 organic solvent Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 235000019419 proteases Nutrition 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 230000009849 deactivation Effects 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- 208000001953 Hypotension Diseases 0.000 description 2
- 102000017055 Lipoprotein Lipase Human genes 0.000 description 2
- 108010013563 Lipoprotein Lipase Proteins 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 208000021822 hypotensive Diseases 0.000 description 2
- 230000001077 hypotensive effect Effects 0.000 description 2
- 108010004032 Bromelains Proteins 0.000 description 1
- 241001005836 Euchloe ausonia Species 0.000 description 1
- 208000007212 Foot-and-Mouth Disease Diseases 0.000 description 1
- 241000710198 Foot-and-mouth disease virus Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000001132 Osteoporosis Diseases 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- -1 acyl glycerin Chemical compound 0.000 description 1
- 125000002252 acyl group Chemical group 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003570 biosynthesizing effect Effects 0.000 description 1
- 235000019835 bromelain Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 238000005461 lubrication Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Images
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Cosmetics (AREA)
Abstract
The invention takes fish skin as a raw material and uses biological enzyme digestion technology to degrease as well as to extract fish collagen from the fish skin. The extraction of the fish collagen includes the steps of: decontamination of the fish skin, cleaning, water distribution, degreasing by lipase, then zymohydrolysis by prolease, enzyme killing and filtration, and the concentration and drying of obtained filtrate, thus finally obtaining the fish collagen.
Description
(1) a kind of extracting method of collagen of fish skin
(2) technical field:
The present invention relates to a kind of fish-skin deep process technology, especially a kind of is raw material with the fish-skin, utilizes the degreasing of biotechnology enzyme incision technology and utilizes the biological enzyme incision technology to extract the method for collagen of fish skin.
(3) background technology:
Main component in the fish-skin is fat and collagen protein.Collagen protein is a kind of important function protein, and it and hyperplasia, differentiation, motion, immunity, joint lubrication, wound healing etc. are closely related.Modern study shows, collagen protein have the proteinaceous nutrient of increasing, preventing osteoporosis, anti-oxidant, delay senility, beauty treatment, function such as hypotensive.The raw material main source of the collagen product of producing is from the mammiferous skin of Lu Sheng such as pig, ox, bone and tendon at present.Cause epiphytotics generations such as mad cow disease, foot and mouth disease along with global ecological environment worsens in recent years, to make the danger of from terrestrial animal skin, bone, extracting collagen protein increase, to extract then safety many of collagen protein as raw material with fish-skin.
Collagen of fish skin can be widely used in industries such as food, healthcare products, makeup, daily-use chemical industry, biosynthesizing, because collagen protein also has excellent biological compatibility and weak antigenicity, collagen protein also has been widely used aspect medical.
Fish skin collagen just obtains extensive studies in countries such as Japan, the U.S., Norway from the nineties in last century, domestic also many after entering 21 century about the research of extracting collagen protein in the fish-skin, but it is no matter still external at home, they produce fish skin collagen all needs to use a large amount of organic solvents or alkali and deviates from fat in the fish-skin, and all there are problems such as serious potential safety hazard and environmental pollution in organic solvent and alkali degreasing.Utilize the biological enzyme incision technology to remove the fat in the fish-skin and extract collagen protein and then can overcome these shortcomings fully.The technology of utilizing degreasing of biological enzyme incision technology and one step of extraction collagen to finish is not at present appeared in the newspapers as yet.
(4) summary of the invention
The purpose of this invention is to provide and a kind ofly need not organic solvent or alkali takes off fat, directly utilize the degreasing of biological enzyme incision technology and extract a kind of method of collagen protein.
Purpose of the present invention can be implemented by following measure.
(5) a kind of extracting method of collagen of fish skin:
1. with fresh fish-skin removal of impurities, clean up.
2. add 2 ~ 5 times water by fresh fish tare weight amount, stir.
3. send above-mentioned suspended matter to retort, be warming up to 35 ℃～70 ℃, regulating pH with calcium hydroxide is 8～10, press 0.1%～5% lipase of tare weight amount, enzymolysis time is 0.5～3 hour, presses 0.1% ~ 5% of fish-skin umber weight again and adds proteolytic enzyme, carries out enzymolysis 3 ~ 8 hours at 35 ~ 70 ℃, the enzymolysis solution that obtains is warming up to 95 ℃, the constant temperature enzyme that goes out;
4. enzymolysis solution is filtered and obtain supernatant and filter residue;
5. supernatant concentration, drying are obtained the ideal collagen of fish skin.
Accompanying drawing of the present invention is a kind of extracting method process flow sheet of collagen of fish skin.
From accompanying drawing, can clearly recognize and utilize the present invention to extract the complete of collagen Process.
Described lipase is: triglyceride lipase, diacylglycerol lipase, an acyl The glycerin fatty enzyme.
Described protease is: trypsase, pepsin, alkali protease, bromelain, In papain, neutral proteinase, the compound protease one or more.
The present invention is relative, and traditional processing technology has the following advantages:
1. adopt the method for the invention successfully to avoid the needed organic solvent of conventional production methods or degrease by alkali processing, efficiently solve environmental pollution and potential safety hazard that traditional handicraft is brought.
2. adopt the collagen of explained hereafter of the present invention through the cutting of protease, contain The small-molecular weight collagen of various fragments not only is beneficial to the absorption of human body utilization, and wherein contains Have much have anti-oxidant, delay senility, the biologically active peptide of the physiological function such as hypotensive, for Develop various functional products new resources are provided.
3. technology production technology of the present invention is simple, with short production cycle. With low cost, the product peace Entirely, nontoxic pair of effect can be widely used in food, health products, cosmetics and medicine neck The territory.
(6) embodiment
Below further specify the whole process that the technical scheme that provided is implemented with example:
Embodiment one:
Accurately take by weighing removal of impurities, clean 1 kilogram on fresh fish-skin, add 2 kilograms of distilled water, stir.Suspended matter is warming up to 35 ℃～70 ℃, regulating pH with calcium hydroxide is 9, add 10 gram triglyceride lipase, enzymolysis time is 2 hours, add 12 gram Sumizyme MPs again, enzymolysis time is 4 hours, is warmed up to 95 ℃ then and keeps making in 10 minutes enzyme deactivation, then enzymolysis solution is filtered and obtain supernatant liquor and filter residue, supernatant liquor obtains collagen of fish skin through concentrated, drying.
Embodiment two:
Accurately take by weighing removal of impurities, clean 2 kilograms on fresh fish-skin, add 3 kilograms of distilled water, stir.Suspended matter is warming up to 35 ℃～70 ℃, regulating pH with calcium hydroxide is 10, add 15 gram diacylglycerol lipase, enzymolysis time is 1.5 hours, add 20 gram papoids again, enzymolysis time is 5 hours, is warmed up to 95 ℃ then and keeps making in 10 minutes enzyme deactivation, then enzymolysis solution is filtered and obtain supernatant liquor and filter residue, supernatant liquor obtains collagen of fish skin through concentrated, drying.
Embodiment three:
Accurately take by weighing removal of impurities, clean 5 kilograms on fresh fish-skin, add 8 kilograms of distilled water, stir, become the suspension state to pearl white.Suspended matter is warming up to 35 ℃～70 ℃, regulating pH with calcium hydroxide is 8, add 20 grams, one acyl glycerin fatty enzyme, enzymolysis time is 1.8 hours, add 30 gram trypsinase again, enzymolysis time is 4 hours, is warmed up to 95 ℃ then and keeps making in 10 minutes enzyme deactivation, then enzymolysis solution is filtered and obtain supernatant liquor and filter residue, supernatant liquor obtains collagen of fish skin through concentrated, drying.
Claims (1)
- One, a kind of extracting method of collagen of fish skin is characterized in that comprising:(1) adopts fresh fish-skin as the raw material that extracts Isin glue collagen, fish-skin through removal of impurities, is cleaned, add 2～5 times water, stir by fresh fish tare weight amount;(2) utilizing calcium hydroxide to regulate pH is 8～10, press 0.1%～5% of fish-skin umber weight and add lipase, carried out the enzymolysis degreasing 0.5～3 hour at 35～70 ℃, press 0.1%～5% of fish-skin umber weight again and add proteolytic enzyme, carried out enzymolysis 3～8 hours at 35～70 ℃, the enzymolysis solution that obtains is warming up to 95 ℃, the constant temperature enzyme that goes out;(3) enzymolysis solution is filtered resulting supernatant liquor through concentrate, dry, obtain the collagen of fish skin powder.(4) extracting method of a kind of collagen of fish skin as claimed in claim 1 is characterized in that described Isin glue collagen can be used as additive and is widely used in food, healthcare products, makeup.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910131265A CN101538602A (en) | 2009-04-13 | 2009-04-13 | Extraction method of fish skin collagen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910131265A CN101538602A (en) | 2009-04-13 | 2009-04-13 | Extraction method of fish skin collagen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101538602A true CN101538602A (en) | 2009-09-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910131265A Pending CN101538602A (en) | 2009-04-13 | 2009-04-13 | Extraction method of fish skin collagen |
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| CN (1) | CN101538602A (en) |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101899491A (en) * | 2010-05-18 | 2010-12-01 | 武汉艾诗妮娜生物科技有限公司 | Method for preparing micromolecular collagen peptide from fish scales by utilizing complex enzyme |
| CN101928744A (en) * | 2010-04-27 | 2010-12-29 | 兰州城市学院 | Extraction of Active Collagen Peptides from Salmon and Trout Wastes |
| CN101926472A (en) * | 2010-07-07 | 2010-12-29 | 青岛波尼亚食品有限公司 | Fish sausage containing nutritional factors and preparation method thereof |
| CN101946907A (en) * | 2010-08-06 | 2011-01-19 | 海南大学 | Method for producing collagen calcium |
| CN102154420A (en) * | 2010-11-11 | 2011-08-17 | 武汉远城科技发展有限公司 | Method for extracting pigskin collagen peptides |
| CN102936611A (en) * | 2012-11-12 | 2013-02-20 | 山东美佳集团有限公司 | Collagen extracted from black butterflyfish skins with scales and application of collagen |
| CN103238870A (en) * | 2013-05-14 | 2013-08-14 | 福建农林大学 | Preparation method of instant fish skin jelly food |
| CN103357060A (en) * | 2013-07-23 | 2013-10-23 | 海南光宇生物科技有限公司 | Method for preparing bacterial cellulose composite fish collagen wound dressing |
| CN103385803A (en) * | 2013-07-23 | 2013-11-13 | 海南光宇生物科技有限公司 | Preparation method of bacterial cellulose compound fish collagen protein facial mask |
| CN103386144A (en) * | 2013-07-23 | 2013-11-13 | 海南光宇生物科技有限公司 | Preparation method of fish collagen combined chitosan biological dressing |
| CN105107815A (en) * | 2015-06-25 | 2015-12-02 | 河南农业大学 | Method for treating illness-dead livestock and poultry by combining chemical method with biological method |
| CN106359631A (en) * | 2016-09-28 | 2017-02-01 | 宁辉 | Pure collagen milk powder and preparation method thereof |
| CN106689948A (en) * | 2016-11-30 | 2017-05-24 | 河北爱怡尚饮品有限公司 | Collagen composite linolenic acid beverage and preparation method thereof |
| CN109097426A (en) * | 2018-08-31 | 2018-12-28 | 宁波海之源生物科技有限公司 | A kind of preparation method of giant salamander protein peptides |
| CN109295138A (en) * | 2018-09-27 | 2019-02-01 | 浙江海洋大学 | A kind of preparation method of monkfish skin peptide and immune regulation application of the prepared monkfish skin peptide |
| CN110218756A (en) * | 2019-05-31 | 2019-09-10 | 嫦娥创新（武汉）生物科技有限公司 | A kind of selenium-rich sturgeon bone peptide extracting method and product with Antiageing effect |
| CN111471733A (en) * | 2020-04-27 | 2020-07-31 | 山西大学 | A kind of preparation method and application of Daxing shark skin polypeptide dry powder |
| CN108728509B (en) * | 2018-08-09 | 2021-07-13 | 浙江海洋大学 | A kind of preparation method of fish skin immunomodulatory peptide of Japanese yellow croaker |
-
2009
- 2009-04-13 CN CN200910131265A patent/CN101538602A/en active Pending
Cited By (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101928744A (en) * | 2010-04-27 | 2010-12-29 | 兰州城市学院 | Extraction of Active Collagen Peptides from Salmon and Trout Wastes |
| CN101928744B (en) * | 2010-04-27 | 2012-08-22 | 兰州城市学院 | Process for extracting active collagen peptide from salmon trout waste |
| CN101899491A (en) * | 2010-05-18 | 2010-12-01 | 武汉艾诗妮娜生物科技有限公司 | Method for preparing micromolecular collagen peptide from fish scales by utilizing complex enzyme |
| CN101926472A (en) * | 2010-07-07 | 2010-12-29 | 青岛波尼亚食品有限公司 | Fish sausage containing nutritional factors and preparation method thereof |
| CN101946907A (en) * | 2010-08-06 | 2011-01-19 | 海南大学 | Method for producing collagen calcium |
| CN102154420A (en) * | 2010-11-11 | 2011-08-17 | 武汉远城科技发展有限公司 | Method for extracting pigskin collagen peptides |
| CN102154420B (en) * | 2010-11-11 | 2013-05-22 | 湖北远成药业有限公司 | Method for extracting pigskin collagen peptides |
| CN102936611A (en) * | 2012-11-12 | 2013-02-20 | 山东美佳集团有限公司 | Collagen extracted from black butterflyfish skins with scales and application of collagen |
| CN102936611B (en) * | 2012-11-12 | 2015-04-29 | 山东美佳集团有限公司 | Collagen extracted from black butterflyfish skins with scales and application of collagen |
| CN103238870A (en) * | 2013-05-14 | 2013-08-14 | 福建农林大学 | Preparation method of instant fish skin jelly food |
| CN103386144B (en) * | 2013-07-23 | 2014-10-01 | 海南光宇生物科技有限公司 | Preparation method of fish collagen combined chitosan biological dressing |
| CN103386144A (en) * | 2013-07-23 | 2013-11-13 | 海南光宇生物科技有限公司 | Preparation method of fish collagen combined chitosan biological dressing |
| CN103385803A (en) * | 2013-07-23 | 2013-11-13 | 海南光宇生物科技有限公司 | Preparation method of bacterial cellulose compound fish collagen protein facial mask |
| CN103385803B (en) * | 2013-07-23 | 2014-11-19 | 海南光宇生物科技有限公司 | Preparation method of bacterial cellulose compound fish collagen protein facial mask |
| CN103357060B (en) * | 2013-07-23 | 2015-01-14 | 海南光宇生物科技有限公司 | Method for preparing bacterial cellulose composite fish collagen wound dressing |
| CN103357060A (en) * | 2013-07-23 | 2013-10-23 | 海南光宇生物科技有限公司 | Method for preparing bacterial cellulose composite fish collagen wound dressing |
| CN105107815A (en) * | 2015-06-25 | 2015-12-02 | 河南农业大学 | Method for treating illness-dead livestock and poultry by combining chemical method with biological method |
| CN106359631B (en) * | 2016-09-28 | 2017-11-03 | 宁辉 | A kind of pure collagen milk powder and preparation method thereof |
| CN106359631A (en) * | 2016-09-28 | 2017-02-01 | 宁辉 | Pure collagen milk powder and preparation method thereof |
| CN106689948A (en) * | 2016-11-30 | 2017-05-24 | 河北爱怡尚饮品有限公司 | Collagen composite linolenic acid beverage and preparation method thereof |
| CN106689948B (en) * | 2016-11-30 | 2021-04-09 | 河北爱怡尚饮品有限公司 | A kind of collagen compound linolenic acid beverage and preparation method thereof |
| CN108728509B (en) * | 2018-08-09 | 2021-07-13 | 浙江海洋大学 | A kind of preparation method of fish skin immunomodulatory peptide of Japanese yellow croaker |
| CN109097426A (en) * | 2018-08-31 | 2018-12-28 | 宁波海之源生物科技有限公司 | A kind of preparation method of giant salamander protein peptides |
| CN109295138A (en) * | 2018-09-27 | 2019-02-01 | 浙江海洋大学 | A kind of preparation method of monkfish skin peptide and immune regulation application of the prepared monkfish skin peptide |
| CN110218756A (en) * | 2019-05-31 | 2019-09-10 | 嫦娥创新（武汉）生物科技有限公司 | A kind of selenium-rich sturgeon bone peptide extracting method and product with Antiageing effect |
| CN111471733A (en) * | 2020-04-27 | 2020-07-31 | 山西大学 | A kind of preparation method and application of Daxing shark skin polypeptide dry powder |
| CN111471733B (en) * | 2020-04-27 | 2023-03-07 | 山西大学 | Preparation method and application of shark skin polypeptide dry powder |
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Open date: 20090923 |