CN101892278A - Method for extracting micromolecular collagen oligopeptide from fish - Google Patents
Method for extracting micromolecular collagen oligopeptide from fish Download PDFInfo
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- CN101892278A CN101892278A CN2009100395556A CN200910039555A CN101892278A CN 101892278 A CN101892278 A CN 101892278A CN 2009100395556 A CN2009100395556 A CN 2009100395556A CN 200910039555 A CN200910039555 A CN 200910039555A CN 101892278 A CN101892278 A CN 101892278A
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Abstract
The invention discloses a method for extracting micromolecular collagen oligopeptide from fish, comprising the following step of: lipase degreasing: putting cleaned and dried scales or fishskin into an extraction pot; evenly mixing by adding water according to weight of 3-15 times; carrying out zymohydrolysis by adding lipase according to 0.01-1 percent of the weight of the scales or the fishskin; and then, cleaning, regulating a pH value, carrying out zymohydrolysis by adding the prolease, blanching, filtering, concentrating and drying to obtain the micromolecular collagen oligopeptide. The prolease comprises the following zymohydrolysis step of: carrying out zymohydrolysis by adding the prolease according to 1-4 percent of the weight of the scales or the fishskin for 1-6 hours at the temperature of 38-68 DEG C. Because the lipase is adopted to degrease the scales or fishskin, the method increases degreasing effect, thereby greatly reducing zymohydrolysis time of the prolease and improving production efficiency; and moreover, the processed collagen has lower molecular weight and is beneficial to being absorbed by a human body.
Description
Technical field
The present invention relates to a kind of extracting method of collagen oligopeptide, specifically be meant a kind of method that adopts the enzymolysis mode from fish, to extract micromolecular collagen oligopeptide.
Background technology
Collagen (Collagen) albumen is a kind of important function protein, and it and hyperplasia, differentiation, motion, immunity, joint lubrication, wound healing etc. are closely related.Because the specific function of collagen protein, its extract has been widely used in industrial circles such as medicine, food, daily-use chemical industry, biosynthesizing, as medical capsule, surgical operation material, edible Gelatinum oxhide, photographic gelatin, makeup etc.The raw material main source of the collagen product of producing is from the mammiferous skin of Lu Sheng such as pig, ox, bone and tendon at present, because of the danger that causes epiphytotics generations such as many mad cow diseases, foot and mouth disease along with global ecological environment worsens in recent years, make extracting collagen protein from terrestrial animal skin, bone increases.American-European, Japan and other countries has been put into effect many rules in succession, and restriction extract from Mammals osteoderms such as ox is used for food medicine etc. to the direct product of influence of human body.Thus, people just begin to seek to extract collagen protein from aquatic animal, thereby, from hydrocoles fish body waste, extract the heat subject that safety, health, harmless collagen protein have become current domestic and international insider's research and development.
Research and analyse through the insider, collagen content accounts for the 25-30% of fish body total protein, mainly be distributed in fish scale and the fish-skin, and the thick collagen protein in the fish scale, fish-skin just accounts for 80%, and all fish scale, fish-skin are thrown away fish scale especially in the general aquatic products processing as waste.Therefore, fully and reasonably utilize the waste fish scale to extract collagen protein and not only can promote the development of fish processing industry, and can make a silk purse out of a sow's ear, reduce environmental pollution, bring benefit to the mankind better.China is maximum in the world freshwater fish culturing state, and according to incompletely statistics, annual discarded fish scale reaches 50,000 tons approximately, and the fish scale resource is quite abundant, and at present domestic existing how tame fish scale acquiring company dries fish scale as a kind of resource exit.External as the also very good Chinese fish scale resource of Japan, and will establish the production base in China and develop the fish scale collagen product jointly.
At present, adopt the enzymolysis mode to extract the working method of micromolecular collagen oligopeptide from fish scale, some processing enterprise does not carry out skimming treatment less than before enzymolysis, so its enzymolysis is chronic, thereby has influenced production efficiency; In order to remedy above-mentioned deficiency, also some enterprise has adopted the scheme of the preceding degreasing of enzymolysis, but owing to adopt salt solution or alcoholic solution to carry out skimming treatment, this scheme degreasing is very not thorough, does not reach its expected effect.Also the collagen molecules amount produced of ubiquity is bigger in above-mentioned enterprise, is unfavorable for absorption by human body.
Summary of the invention
In order to overcome above-mentioned deficiency, the object of the present invention is to provide the method for from fish, extracting micromolecular collagen oligopeptide that a kind of degreasing effect is good, production efficiency is high.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is: extract the method for micromolecular collagen oligopeptide from fish, it may further comprise the steps:
1, lipase degreasing:
To clean air dried fish scale or fish-skin and put into extractor, by the 3-15 of its weight extraordinarily water stir, the 0.01%-1% that presses fish scale or fish-skin weight adds lipase, carries out enzymolysis;
2, clean:
After bleeding off the enzymolysis solution in the extractor, in extractor, add water flushing fish scale or fish-skin 3-5 time;
3, adjust pH value:
In extractor, add entry, amount of water be fish scale or fish-skin weight 3-15 doubly, be warming up to 42 ℃-68 ℃, be that the NaOH of 3%-17% and pH value that HCL will extract pot liquid are adjusted to 6.8-9.9 with concentration;
4, add proteolytic enzyme and carry out enzymolysis:
Be cooled to 38 ℃-68 ℃ again, add proteolytic enzyme by the 1%-4% of fish scale or fish-skin weight and carry out enzymolysis, enzymolysis time is 1-6 hour;
5, the enzyme that goes out:
After enzymolysis finishes enzymolysis solution is warming up to 70 ℃-95 ℃, kept about 30 minutes, behind the enzyme that goes out, being cooled to 65 ℃ of adjusting pH is 6.5-8.5;
6, filter, concentrate, drying obtains micromolecular collagen oligopeptide.
Used lipase is the triacylglycerol Acyl-hydrolase in the aforesaid method, and its catalysis natural substrate fat hydrolysis generates lipid acid, glycerine and monoglyceride or diester, and lipase essentially consist unit only is an amino acid, has only a polypeptide chain usually; Proteolytic enzyme adopts one or several in stomach en-, trypsinase, papoid, neutral protease, aspartic protease, Sumizyme MP, bromeline, the compound protease.
Beneficial effect of the present invention is: owing to adopt lipase that fish scale or fish-skin are carried out skimming treatment, so improved degreasing effect, thereby not only shortened the enzymolysis time of proteolytic enzyme greatly, improved production efficiency, and the collagen molecules amount that processes is littler, helps absorption by human body.
Specific embodiment
Embodiment 1
Extract the method for micromolecular collagen oligopeptide from fish, it may further comprise the steps:
1, lipase degreasing:
To clean air dried fish scale or fish-skin and put into extractor, by its weight 3 extraordinarily water stir, add 0.01% lipase and carry out enzymolysis;
2, clean:
After bleeding off the enzymolysis solution in the extractor, in extractor, add water flushing fish scale or fish-skin 3 times;
3, adjust pH value:
Add entry in extractor, amount of water is fish scale or fish-skin weight 3 times, is warming up to 42 ℃, is that the pH value that 3% NaOH and HCL will extract pot liquid is adjusted to 6.8 with concentration;
4, add proteolytic enzyme and carry out enzymolysis:
Be cooled to 38 ℃ again, add proteolytic enzyme by 1% of fish scale or fish-skin weight and carry out enzymolysis, enzymolysis time is 6 hours;
5, the enzyme that goes out:
After enzymolysis finishes enzymolysis solution is warming up to 70 ℃, kept about 30 minutes, behind the enzyme that goes out, being cooled to 65 ℃ of adjusting pH is 6.5;
6, filter, concentrate, drying obtains micromolecular collagen oligopeptide.
Lipase is the triacylglycerol Acyl-hydrolase, and its catalysis natural substrate fat hydrolysis generates lipid acid, glycerine and monoglyceride or diester, and lipase essentially consist unit only is an amino acid, has only a polypeptide chain usually; Proteolytic enzyme adopts stomach en-.
Embodiment 2
Extract the method for micromolecular collagen oligopeptide from fish, it may further comprise the steps:
1, lipase degreasing:
To clean air dried fish scale or fish-skin and put into extractor, by its weight 9 extraordinarily water stir, press 0.05% of fish scale or fish-skin weight and add lipase, carry out enzymolysis;
2, clean:
After bleeding off the enzymolysis solution in the extractor, in extractor, add water flushing fish scale or fish-skin 4 times;
3, adjust pH value:
In extractor, add entry, amount of water be fish scale or fish-skin weight 3-15 doubly, be warming up to 42 ℃-68 ℃, be that the pH value that 10% NaOH and HCL will extract pot liquid is adjusted to 8 with concentration;
4, add proteolytic enzyme and carry out enzymolysis:
Be cooled to 53 ℃ again, add proteolytic enzyme by 2.5% of fish scale or fish-skin weight and carry out enzymolysis, enzymolysis time is 4 hours;
5, the enzyme that goes out:
After enzymolysis finishes enzymolysis solution is warming up to 82 ℃, kept about 30 minutes, behind the enzyme that goes out, being cooled to 65 ℃ of adjusting pH is 7.5;
6, filter, concentrate, drying obtains micromolecular collagen oligopeptide.
In the aforesaid method, proteolytic enzyme adopts trypsinase.
Embodiment 3
Extract the method for micromolecular collagen oligopeptide from fish, it may further comprise the steps:
2, lipase degreasing:
To clean air dried fish scale or fish-skin and put into extractor, by its weight 15 extraordinarily water stir, press 1% of fish scale or fish-skin weight and add lipase, carry out enzymolysis;
2, clean:
After bleeding off the enzymolysis solution in the extractor, in extractor, add water flushing fish scale or fish-skin 5 times;
3, adjust pH value:
Add entry in extractor, amount of water is fish scale or fish-skin weight 15 times, is warming up to 68 ℃, is that the pH value that 17% NaOH and HCL will extract pot liquid is adjusted to 9.9 with concentration;
4, add proteolytic enzyme and carry out enzymolysis:
Be cooled to 68 ℃ again, add proteolytic enzyme by 4% of fish scale or fish-skin weight and carry out enzymolysis, enzymolysis time is 1 hour;
5, the enzyme that goes out:
After enzymolysis finishes enzymolysis solution is warming up to 95 ℃, kept about 30 minutes, behind the enzyme that goes out, being cooled to 65 ℃ of adjusting pH is 8.5;
6, filter, concentrate, drying obtains micromolecular collagen oligopeptide.
In the aforesaid method, proteolytic enzyme adopts stomach en-.
Claims (1)
1. method of from fish, extracting micromolecular collagen oligopeptide, it is characterized in that: extract the method for micromolecular collagen oligopeptide from fish, it may further comprise the steps:
1. lipase degreasing:
To clean air dried fish scale or fish-skin and put into extractor, by the 3-15 of its weight extraordinarily water stir, the 0.01%-1% that presses fish scale or fish-skin weight adds lipase, carries out enzymolysis;
2. clean:
After bleeding off the enzymolysis solution in the extractor, in extractor, add water flushing fish scale or fish-skin 3-5 time;
3. adjust pH value:
In extractor, add entry, amount of water be fish scale or fish-skin weight 3-15 doubly, be warming up to 42 ℃-68 ℃, be that the NaOH of 3%-17% and pH value that HCL will extract pot liquid are adjusted to 6.8-9.9 with concentration;
4. add proteolytic enzyme and carry out enzymolysis:
Be cooled to 38 ℃-68 ℃ again, add proteolytic enzyme by the 1%-4% of fish scale or fish-skin weight and carry out enzymolysis, enzymolysis time is 1-6 hour;
5. enzyme goes out:
After enzymolysis finishes enzymolysis solution is warming up to 70 ℃-95 ℃, kept about 30 minutes, behind the enzyme that goes out, being cooled to 65 ℃ of adjusting pH is 6.5-8.5;
6. filter, concentrate, drying obtains micromolecular collagen oligopeptide.
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| CN2009100395556A CN101892278A (en) | 2009-05-18 | 2009-05-18 | Method for extracting micromolecular collagen oligopeptide from fish |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154421A (en) * | 2010-12-15 | 2011-08-17 | 南通市优普保鲜技术有限公司 | Method for extracting collagen from fish processing waste including skin, bones and scale |
| CN102154424A (en) * | 2011-01-14 | 2011-08-17 | 国家海洋局第三海洋研究所 | Process for preparing micro-molecular fish scale collagen peptides |
| CN102366124A (en) * | 2011-07-28 | 2012-03-07 | 浙江省海洋开发研究院 | Fish-skin degreasing method |
| CN102578363A (en) * | 2012-03-29 | 2012-07-18 | 江南大学 | Method for preparing small-molecule collagen polypeptide powder by using poultry skin and application of small-molecule collagen polypeptide powder in fruit juice beverage |
| CN103388017A (en) * | 2013-08-06 | 2013-11-13 | 国家海洋局第三海洋研究所 | Preparation technology of fish skin collagen oligopeptide |
| CN104256771A (en) * | 2014-08-26 | 2015-01-07 | 沈华辉 | A fruit-flavor beverage rich in collagen peptide and a processing process thereof |
| CN104293868A (en) * | 2013-07-18 | 2015-01-21 | 张明焕 | Preparation method of fish scale collagen peptide |
| CN104388512A (en) * | 2014-11-28 | 2015-03-04 | 桂林市和胤祥新型材料有限公司 | Method for preparing hydrolyzed collagen |
| CN104962599A (en) * | 2015-07-30 | 2015-10-07 | 太阳树（厦门）生物工程有限公司 | Technique for extracting collagen from tilapia scales |
| CN105995908A (en) * | 2016-05-27 | 2016-10-12 | 广东省农业科学院蚕业与农产品加工研究所 | Preparation method of peptide base material for meat products and flavored meat product prepared from peptide base material |
| CN108617836A (en) * | 2018-05-10 | 2018-10-09 | 吴海旭 | A kind of energy cake peanut taste pressed candy and preparation method thereof |
| CN110269128A (en) * | 2019-07-22 | 2019-09-24 | 中肽生物科技(大连)有限公司 | A kind of preparation method of nanoscale small molecule complex peptides |
| CN111574617A (en) * | 2020-04-26 | 2020-08-25 | 无锡可尚生物科技有限公司 | Collagen peptide for preventing and treating senile osteoporosis and preparation method thereof |
| CN108728509B (en) * | 2018-08-09 | 2021-07-13 | 浙江海洋大学 | Preparation method of fish skin immunoregulation peptide of Nibea japonica |
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2009
- 2009-05-18 CN CN2009100395556A patent/CN101892278A/en active Pending
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154421A (en) * | 2010-12-15 | 2011-08-17 | 南通市优普保鲜技术有限公司 | Method for extracting collagen from fish processing waste including skin, bones and scale |
| CN102154424A (en) * | 2011-01-14 | 2011-08-17 | 国家海洋局第三海洋研究所 | Process for preparing micro-molecular fish scale collagen peptides |
| CN102154424B (en) * | 2011-01-14 | 2013-05-08 | 国家海洋局第三海洋研究所 | Process for preparing micro-molecular fish scale collagen peptides |
| CN102366124A (en) * | 2011-07-28 | 2012-03-07 | 浙江省海洋开发研究院 | Fish-skin degreasing method |
| CN102578363A (en) * | 2012-03-29 | 2012-07-18 | 江南大学 | Method for preparing small-molecule collagen polypeptide powder by using poultry skin and application of small-molecule collagen polypeptide powder in fruit juice beverage |
| CN104293868A (en) * | 2013-07-18 | 2015-01-21 | 张明焕 | Preparation method of fish scale collagen peptide |
| CN103388017B (en) * | 2013-08-06 | 2014-12-17 | 国家海洋局第三海洋研究所 | Preparation technology of fish skin collagen oligopeptide |
| CN103388017A (en) * | 2013-08-06 | 2013-11-13 | 国家海洋局第三海洋研究所 | Preparation technology of fish skin collagen oligopeptide |
| CN104256771A (en) * | 2014-08-26 | 2015-01-07 | 沈华辉 | A fruit-flavor beverage rich in collagen peptide and a processing process thereof |
| CN104388512A (en) * | 2014-11-28 | 2015-03-04 | 桂林市和胤祥新型材料有限公司 | Method for preparing hydrolyzed collagen |
| CN104962599A (en) * | 2015-07-30 | 2015-10-07 | 太阳树（厦门）生物工程有限公司 | Technique for extracting collagen from tilapia scales |
| CN105995908A (en) * | 2016-05-27 | 2016-10-12 | 广东省农业科学院蚕业与农产品加工研究所 | Preparation method of peptide base material for meat products and flavored meat product prepared from peptide base material |
| CN108617836A (en) * | 2018-05-10 | 2018-10-09 | 吴海旭 | A kind of energy cake peanut taste pressed candy and preparation method thereof |
| CN108728509B (en) * | 2018-08-09 | 2021-07-13 | 浙江海洋大学 | Preparation method of fish skin immunoregulation peptide of Nibea japonica |
| CN110269128A (en) * | 2019-07-22 | 2019-09-24 | 中肽生物科技(大连)有限公司 | A kind of preparation method of nanoscale small molecule complex peptides |
| CN111574617A (en) * | 2020-04-26 | 2020-08-25 | 无锡可尚生物科技有限公司 | Collagen peptide for preventing and treating senile osteoporosis and preparation method thereof |
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Application publication date: 20101124 |