CN102533914B - High-purity fishy smell and foreign odor-free fish collagen protein peptide and preparation method thereof - Google Patents
High-purity fishy smell and foreign odor-free fish collagen protein peptide and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a high-purity fishy smell and foreign odor-free fish collagen protein peptide and a preparation method thereof. The preparation method comprises the following steps: cleaning fish skins, and then cutting into blocks and mincing; performing stirring treatment with NaCl solution; centrifugally degreasing to remove paraprotein; adding water to regulate the pH value of initial slurry to be 8.0-8.5; performing combined gradient enzymolysis on alkali protease and neutral protease; deactivating enzyme after the enzymolysis is ended; performing adsorption bleaching by activated carbon; and then performing rough filtration, fine filtration, evaporation concentration and spray drying to prepare the high-purity fishy smell and foreign odor-free fish collagen protein peptide. The high-purity fishy smell and foreign odor-free fish collagen protein peptide disclosed by the invention has a simple process and is easy to industrial production; fishy smell, foreign odor and non-collagen proteins are fully removed in production; the low-temperature biological enzymolysis technology is adopted, and other substances are not added, thereby, the product quality is improved; an obtained fish collagen protein peptide powder has the characteristics the content of amino acid hydroxyproline is not less than 9 percent, and the average molecular weight is lower than 1000Dal; no fishy smell or foreign odor cannot generated, and obvious fishy smell or foreign odor also cannot be generated through heating treatment under the acid conditions. The high-purity fishy smell and foreign odor-free fish collagen protein peptide can be widely applied to processing of various foods and cosmetics as a green functional ingredient.
Description
Technical field
The present invention relates to fishery products waste high added value process and utilization technology field, be specifically related to tilapia processing waste-fish-skin pre-treatment, prozyme gradient enzymolysis, prepare the Isin glue collagen peptide of high purity without off-flavors, this peptide in acid condition heat treated also can not produce off-flavors, can be widely used as the raw material of protective foods, bread and cheese and makeup.
Background technology
Collagen protein is human body skin, bone, the most important structural constituent of reticular tissue.Science proves, adult's nature every day loss collagen protein about about 3 ~ 5 grams.As supply not in time, histocyte can be caused to can not get the raw material of synthetic protein on the one hand, on the other hand nutritive substance VITAMIN, trace element etc. for want of carrier not easily arrive corresponding cell and organ makes cell can not get nutritional support, internal antibody is caused to reduce, function of immune system reduces, endocrine system disorder, therefore medical expert thinks both at home and abroad: collagen deficiency disease, finally causes aging and the many diseases of health to volley.Collagen protein because of its significantly anti-oxidant, anti-aging, replenish the calcium and protect calcium and promote skeleton development, improve body immunity, promote the superior functionalitys such as skin cells regeneration, be widely used in the fields such as protective foods, cosmetics and healthcare products.The women of American-European, Japan and nearly 7 one-tenth of Korea S, the women of nearly 5 one-tenth of Taiwan throughout the year oral or external application collagen product improves looks and keeps healthy.
Traditional collagen protein generally with the skin of the animal such as pig and ox and bone for raw material.Because the collagen protein extracted from livestock and poultry animal has certain limitation in specific absorption (research display collagen protein enteron aisle and skin absorptance are only 2 ~ 4%), and along with the fear to the contour pathogenic infections disease of mad cow disease, foot and mouth disease and bird flu, from seawater or fresh-water fishes body waste--extract the main flow that security is high, specific absorption is high, biological activity is stronger collagen peptide has become collagen product industry development fish-skin and fish scale.Research and utilization fish-skin, fish scale extract the focus that collagen peptide has become research and development both at home and abroad.
Existing Isin glue collagen peptide working method mainly contains some problem following: the collagen peptide extracting method employing acid that (1) is traditional or alkaline degradation collagen protein extract, these methods can cause partial amino-acid to destroy in the middle of degradation process, Product Safety is poor, and biological activity is lost; (2) adopt biological enzymolysis technology to prepare Isin glue collagen peptide, enzyme combination selection is undesirable, and production efficiency is low, molecular weight product is higher, add a large amount of technical-grade hydrochloric acid, sodium hydroxide adjust ph in enzymolysis process, affect product quality, and add product purification difficulty and production cost; (3) due to Feedstock treating not in time or production process take off raw meat and go peculiar smell not in place, the Isin glue collagen product off-flavors weight developed, limit its application in derived product; Or de-raw meat goes peculiar smell process for refining very complicated, as Chinese patent 200810138406.0 discloses a kind of fish collagen peptides ion exchange chromatography fishy smell elimination technique, adopt Isin glue collagen peptide pre-treatment, transition, upper carboxymethyl cellulose exchange column, pure water wash-out, desalination, reconcentration is dried to the Gly-His-Lys of de-fishy smell; The method that Chinese patent 200810106710.7 discloses and a kind ofly utilizes deliming, the dry fish scale of degreasing is Raw material processing fish collagen oligopeptide, it is refining adopts diatomite filtration, absorption with macroporous adsorbent resin, cationic exchange resin adsorption, anionite-exchange resin absorption, elution, bed filtration membrane filtering method; Chinese patent 200610078017.4 discloses a kind of fish-skin, fish-bone of utilizing for the industrialized preparing process of waste collagen peptide, it is refining comprises that centrifugation is removed slag, raw meat is removed in oil-water centrifugal separator de-oiling, micro-filtration ultra filtering clarifying, 5% powder activated carbon decolouring, filtration procedure; These method complex process, operating process is loaded down with trivial details, is unfavorable for suitability for industrialized production.
Summary of the invention
In order to solve above-mentioned the deficiencies in the prior art part, primary and foremost purpose of the present invention is to provide a kind of low temperature controlled enzymatic hydrolysis technology to prepare the method for high purity without the Isin glue collagen peptide of off-flavors.
Another object of the present invention is the Isin glue collagen peptide providing a kind of aforesaid method to prepare.
The present invention is realized by following technical proposals: a kind of high purity, without the preparation method of the Isin glue collagen peptide of off-flavors, comprises following operation steps:
(1) fish-skin prepares: wash for subsequent use by fresh fish-skin, or freezes acid discharge by anxious for fresh fish-skin, wash before processing thaw for subsequent use;
(2) degreasing, M-band is removed: by fish-skin stripping and slicing for subsequent use, rubbing, the mass percent concentration adding 4 ~ 8 times of fish-skin weight is the NaCl solution stir process 8 ~ 12h of 2 ~ 4%, with the centrifugal 10 ~ 20min of 4000 ~ 6000r/min rotating speed, remove waste liquid, water sediment fraction being added 50 ~ 60 DEG C of 2 ~ 3 times of sediment fraction weight stirs 25 ~ 35min, again with the centrifugal 10 ~ 20min of 4000 ~ 6000r/min rotating speed, remove waste liquid, get precipitation for subsequent use;
(3) enzymolysis: precipitation is added 50 ~ 55 DEG C of water and stir, obtains fish-skin slurry; Ensure that in fish-skin slurry, protein quality percentage composition is 8% ~ 15%, control constant temperature 50 ~ 52 DEG C, regulate fish-skin slurry slurry pH value to 8.0 ~ 8.5, after adding the Sumizyme MP A stirring hydrolysis 1 ~ 3h of protein quality 0.05 ~ 0.3% in fish-skin slurry, then the neutral protease B adding protein quality 0.1 ~ 0.3% in fish-skin slurry stirs hydrolysis 2 ~ 5h;
(4) to go out enzyme, decolouring: after enzymolysis terminates, be warming up to 80 ~ 85 DEG C, add the activated carbon powder stirring and evenly mixing of fish-skin weight 2 ~ 4% in fish-skin slurry slurries, under 80 ~ 85 DEG C of constant temperatures, stir 20 ~ 40min with 30 ~ 60r/min, realize going out enzyme and adsorption bleaching one step complete;
(5) coarse filtration, essence filter: the slurries containing activated carbon after go out enzyme and adsorption bleaching are pumped into plate-frame type diatomaceous earth filter coarse filtration, clear liquid after press filtration is again through the filter of plate and frame cardboard filter essence, remove activated carbon remaining fine particulates and high molecular weight protein (peptide), obtain the Isin glue collagen peptide permeate of clear;
(6) evaporation concentration: Isin glue collagen peptide permeate is concentrated into by economic benefits and social benefits falling film evaporation thickener the concentrated solution that solid quality content is 35 ~ 45%;
(7) spraying dry: concentrated solution drying tower is dry, it is 160 ~ 170 DEG C that inlet temperature controls, and it is 80 ~ 90 DEG C that temperature out controls, and dry end, namely obtains collagen of fish skin Gly-His-Lys finished product.
The described fresh fish-skin of step (1) is the fish-skin of fresh tilapia after peeling is scaled of green non-pollution cultivation; It is under subzero 38 DEG C of conditions, carry out urgency freeze acid discharge that described urgency freezes acid discharge.
Step (1) ~ (3) described water is deionized water.
Step (2) described centrifugal be adopt separating centrifuge carry out centrifugal; The filtering accuracy of the described plate and frame cardboard filter of step (5) is 0.1um.
Step (3) described Sumizyme MP A and neutral protease B is the commercially available food-grade albumen enzyme extracted from plant, animal or microorganism, and two kinds of enzyme combination gradients use, and can significantly improve product yield and improve product special flavour.Described Sumizyme MP A is preferably Sumizyme MP Alcalase, Sumizyme MP Protex-6L or Pang Bo food grade Sumizyme MP; Described neutral protease B is preferably neutral protease Neutrase, neutral protease Protex.7L, Pang Bo food-grade neutral protease, papoid or bromeline.
Step (4) described activated carbon is compound activated carbon; The fineness of described activated carbon powder is 200 ~ 300 orders.
The described drying tower of step (4) adopts Centrafugal spray drying tower, directly can enter the Isin glue collagen Gly-His-Lys product that tablets press obtains different specific weight and granular size after spraying dry.
The described Isin glue collagen Gly-His-Lys of step (7) is in butt: protein quality content >=90% (reduction factor 5.79), characteristic amino acid oxyproline mass content >=9%, molecular-weight average is lower than 1000Dal.
A kind of Isin glue collagen Gly-His-Lys prepared according to aforesaid method.This Isin glue collagen Gly-His-Lys is without off-flavors, and heat treated also can not produce obvious fishy smell, peculiar smell in acid condition, can be widely used in varieties of food items processing and makeup as green functional batching.
Compared with prior art, tool has the following advantages and beneficial effect in the present invention:
(1) the present invention adopts fresh fish-skin or suddenly freezes the fish-skin after thawing is raw material, by rubbing fish-skin before enzymolysis, NaCl solution stir process, centrifugal segregation fat, fat derivant and noncollagen protein technique, reduce on the one hand the impact on product special flavour and follow-up preparation technology of fat and derivative thereof as much as possible, because fat derivant (aldehyde, ketone, Trimethylamine 99 etc.) has unpleasant odor, and fat remains in slurries, subsequent filter technique can be affected, cause deterioration in quality; The removal of noncollagen protein is conducive to the purity improving enzymolysis raw material collagen protein on the other hand, impels the purity of finished product Isin glue collagen peptide to improve, has ensured the high biological activity of product; Do not adopt alkali lye, acid solution, organic solvent process to avoid the destruction of activeconstituents in product and the residual of objectionable impurities fully simultaneously yet; The collagen of fish skin peptide product characteristic amino acid hydroxyproline content that the present invention obtains is not less than 9%, even reaches more than 10%, far above collagen of fish skin peptide product hydroxyproline content 6 ~ 7% level on the market.
(2) adjustment initial slurry pH value to 8.0 ~ 8.5, Sumizyme MP and neutral protease combination gradient enzymolysis is adopted in enzymolysis process of the present invention, the Action advantage of enzyme combination can be given full play to, improve enzymolysis efficiency and product yield, and avoid introducing too much acid solution, alkali lye, improve product special flavour; If not adjust ph completely, the efficient Action advantage of enzyme can not give full play to, if whole process adjust ph, obviously can increase refining difficulty and the production cost of product.
(3) the present invention is by decolorization adsorption, coarse filtration, essence filter refining purification collagen of fish skin peptide further, control molecular weight product size, improve product quality, wherein essence filter adopts plate and frame cardboard filter, realize controlling permeate molecular size range by controlled filter precision, easy to operate, be easy to industrialization.
(4) the collagen of fish skin peptide product purity of the present invention's acquisition is high, excellent flavor, without off-flavors, heat treated also can not produce obvious fishy smell, peculiar smell in acid condition, can be widely used in, in varieties of food items, makeup, not affecting finished product characteristic as high-quality base-material.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1
(1) fish-skin prepares: the fish-skin deionized water after fresh tilapia peeling being scaled is for subsequent use after cleaning;
(2) degreasing, remove M-band: by fish-skin stripping and slicing for subsequent use, rubbing, add the NaCl solution stir process 12h of the mass percentage concentration 4% of 4 times of fish-skin weight, adopt separating centrifuge with the centrifugal 10min of 6000r/min rotating speed, remove waste liquid; Sediment fraction is added the deionized water and stirring 30min of 60 DEG C of 2 times of sediment fraction weight, then with the centrifugal 20min of 4000r/min rotating speed, removes waste liquid, get precipitation for subsequent use;
(3) enzymolysis: precipitation is added 53 DEG C of deionized water and stirring evenly, obtains fish-skin slurry; Ensure that in fish-skin slurry, protein quality content is 8%, control constant temperature 50 DEG C, regulate fish-skin slurry slurry pH value to 8.0, after the Sumizyme MP Alcalase adding protein quality 0.05% in fish-skin slurry stirs and is hydrolyzed 3h, then add protein quality 0.3% neutral protease Neutrase stirring hydrolysis 2h in fish-skin slurry;
(4) to go out enzyme, decolouring: after enzymolysis terminates, be warming up to 80 DEG C, add fish-skin weight 2% activated carbon powder (fineness is 200 orders) stirring and evenly mixing in fish-skin slurry slurries, under 80 DEG C of constant temperatures, stir 30min with 30r/min, realize going out enzyme and adsorption bleaching one step complete;
(5) coarse filtration, essence filter: pump into plate-frame type diatomaceous earth filter coarse filtration containing activated carbon slurries after will go out enzyme, adsorption bleaching, clear liquid after press filtration is again through the filter of plate and frame cardboard filter (filtering accuracy 0.1um) essence, remove activated carbon remaining fine particulates and high molecular weight protein (peptide), obtain the Isin glue collagen peptide permeate of clear;
(6) evaporation concentration: Isin glue collagen peptide permeate is concentrated into by economic benefits and social benefits falling film evaporation thickener the concentrated solution that solid content is 35%;
(7) spraying dry: concentrated solution drying tower is dry, it is 160 DEG C that inlet temperature controls, and it is 80 DEG C that temperature out controls, and dry end, namely obtains collagen of fish skin Gly-His-Lys finished product.
Embodiment 2
(1) fish-skin prepares: the fish-skin deionized water after fresh tilapia peeling being scaled is for subsequent use after cleaning;
(2) degreasing, remove M-band: by fish-skin stripping and slicing for subsequent use, rubbing, the mass percentage concentration adding 8 times of fish-skin weight is the NaCl solution stir process 8h of 2%, adopt separating centrifuge with the centrifugal 10min of 4000r/min rotating speed, remove waste liquid; Sediment fraction is added the deionized water and stirring 30min of 50 DEG C of 3 times of sediment fraction weight, then with the centrifugal 10min of 6000r/min rotating speed, removes waste liquid, get precipitation for subsequent use;
(3) enzymolysis: precipitation is added 55 DEG C of deionized water and stirring evenly, obtains fish-skin slurry; Ensure that in fish-skin slurry, protein quality content is 15%, control constant temperature 52 DEG C, regulate fish-skin slurry slurry pH value to 8.5, after the Sumizyme MP Protex-6L adding protein quality content 0.3% in fish-skin slurry stirs and is hydrolyzed 1h, then add protein content 0.1% neutral protease Protex.7L stirring hydrolysis 5h in fish-skin slurry;
(4) to go out enzyme, decolouring: after enzymolysis terminates, be warming up to 85 DEG C, add fish-skin weight 4% activated carbon powder (fineness is 200 orders) stirring and evenly mixing in fish-skin slurry slurries, under 85 DEG C of constant temperatures, stir 20min with 60r/min, realize going out enzyme and adsorption bleaching one step complete;
(5) coarse filtration, essence filter: pump into plate-frame type diatomaceous earth filter coarse filtration containing activated carbon slurries after will go out enzyme, adsorption bleaching, clear liquid after press filtration is again through the filter of plate and frame cardboard filter (filtering accuracy 0.1um) essence, remove activated carbon remaining fine particulates and high molecular weight protein (peptide), obtain the Isin glue collagen peptide permeate of clear;
(6) evaporation concentration: Isin glue collagen peptide permeate is concentrated into by economic benefits and social benefits falling film evaporation thickener the concentrated solution that solid content is 45%;
(7) spraying dry: concentrated solution drying tower is dry, it is 170 DEG C that inlet temperature controls, and it is 90 DEG C that temperature out controls, and dry end, namely obtains collagen of fish skin Gly-His-Lys finished product.
Embodiment 3
(1) fish-skin prepare: the fish-skin after fresh tilapia peeling being scaled freezes acid discharge in subzero 38 DEG C of urgency, processing before with deionized water clean thaw for subsequent use;
(2) degreasing, M-band is removed: by fish-skin stripping and slicing for subsequent use, rubbing, the mass percentage concentration adding 6 times of fish-skin weight is the NaCl solution stir process 10h of 3%, adopt separating centrifuge with the centrifugal 15min of 5000r/min rotating speed, remove waste liquid, sediment fraction is added 55 DEG C of deionized water and stirring 30min of 3 times of sediment fraction weight, again with the centrifugal 15min of 5000r/min rotating speed, remove waste liquid, get precipitation for subsequent use;
(3) enzymolysis: precipitation is added 53 DEG C of deionized water and stirring evenly, obtains fish-skin slurry; Ensure that in fish-skin slurry, protein quality content is 10%, control constant temperature 50 DEG C, regulate fish-skin slurry slurry pH value to 8.0, after the Pang Bo food grade Sumizyme MP (Bacillus licheniformis2709) adding protein quality 0.15% in fish-skin slurry stirs and is hydrolyzed 2h, then the Pang Bo food-grade neutral protease adding protein quality 0.2% in fish-skin slurry stirs hydrolysis 3h;
(4) to go out enzyme, decolouring: after enzymolysis terminates, be warming up to 80 DEG C, add fish-skin weight 3% activated carbon powder (fineness is 300 orders) stirring and evenly mixing in fish-skin slurry slurries, under 80 DEG C of constant temperatures, stir 40min with 40r/min, realize going out enzyme and adsorption bleaching one step complete;
(5) coarse filtration, essence filter: pump into plate-frame type diatomaceous earth filter coarse filtration containing activated carbon slurries after will go out enzyme, adsorption bleaching, clear liquid after press filtration is again through the filter of plate and frame cardboard filter (filtering accuracy 0.1um) essence, remove activated carbon remaining fine particulates and high molecular weight protein (peptide), obtain the Isin glue collagen peptide permeate of clear;
(6) evaporation concentration: Isin glue collagen peptide permeate is concentrated into by economic benefits and social benefits falling film evaporation thickener the concentrated solution that solid content is 40%;
(7) spraying dry: concentrated solution drying tower is dry, it is 165 DEG C that inlet temperature controls, and it is 85 DEG C that temperature out controls, and dry end, namely obtains collagen of fish skin Gly-His-Lys finished product.
Embodiment 4
(1) fish-skin prepare: the fish-skin after fresh tilapia peeling being scaled freezes acid discharge in subzero 38 DEG C of urgency, processing before with deionized water clean thaw for subsequent use;
(2) degreasing, M-band is removed: by fish-skin stripping and slicing for subsequent use, rubbing, the mass percent concentration adding 7 times of fish-skin weight is the NaCl solution stir process 9h of 3%, adopt separating centrifuge with the centrifugal 20min of 5000r/min rotating speed, remove waste liquid, sediment fraction is added the deionized water and stirring 35min of 55 DEG C of 3 times of sediment fraction weight, again with the centrifugal 20min of 5000r/min rotating speed, remove waste liquid, get precipitation for subsequent use;
(3) enzymolysis: precipitation is added 55 DEG C of deionized water and stirring evenly, obtains fish-skin slurry; Ensure that in fish-skin slurry, protein quality content is 13%, control constant temperature 50 DEG C, regulate fish-skin slurry slurry pH value to 8.3, after the Sumizyme MP Alcalase adding protein quality content 0.15% in fish-skin slurry stirs and is hydrolyzed 2h, then the papoid adding protein quality content 0.2% in fish-skin slurry stirs hydrolysis 3h;
(4) to go out enzyme, decolouring: after enzymolysis terminates, be warming up to 82 DEG C, add fish-skin weight 3% activated carbon powder (fineness is 200 orders) stirring and evenly mixing in fish-skin slurry slurries, under 82 DEG C of constant temperatures, stir 30min with 50r/min, realize going out enzyme and adsorption bleaching one step complete;
(5) coarse filtration, essence filter: pump into plate-frame type diatomaceous earth filter coarse filtration containing activated carbon slurries after the enzyme that goes out, adsorption bleaching, clear liquid after press filtration is again through the filter of plate and frame cardboard filter (filtering accuracy 0.1um) essence, remove activated carbon remaining fine particulates and high molecular weight protein (peptide), obtain the Isin glue collagen peptide permeate of clear;
(6) evaporation concentration: Isin glue collagen peptide permeate is concentrated into by economic benefits and social benefits falling film evaporation thickener the concentrated solution that solid content is 42%;
(7) spraying dry: concentrated solution drying tower is dry, it is 165 DEG C that inlet temperature controls, and it is 85 DEG C that temperature out controls, and dry end, namely obtains collagen of fish skin Gly-His-Lys finished product.
The method adopting GB/T 5009.5 to specify measures the obtained collagen of fish skin Gly-His-Lys total nitrogen content of embodiment 1 ~ 4, is multiplied by protein reduction factor 5.79 and is converted to protein content; GB/T 9695.23-2008 " meat quail hydroxyproline content mensuration " is adopted to analyze the obtained collagen of fish skin Gly-His-Lys hydroxyproline content of embodiment 1 ~ 4; Adopt GB/T 22729-2008 appendix A high performance gel filtration chromatography to analyze the obtained collagen of fish skin Gly-His-Lys molecular-weight average of embodiment 1 ~ 4, the results are shown in Table 1.
The collagen of fish skin Gly-His-Lys analyzing and testing result that table 1 embodiment 1 ~ 4 is obtained
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from spirit of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (7)
1. high purity is without a preparation method for the Isin glue collagen peptide of off-flavors, it is characterized in that comprising following operation steps:
(1) fish-skin prepares: wash for subsequent use by fresh fish-skin, or freezes acid discharge by anxious for fresh fish-skin, wash before processing thaw for subsequent use;
(2) degreasing, M-band is removed: by fish-skin stripping and slicing for subsequent use, rubbing, the mass percent concentration adding 4 ~ 8 times of fish-skin weight is the NaCl solution stir process 8 ~ 12h of 2 ~ 4%, with the centrifugal 10 ~ 20min of 4000 ~ 6000r/min rotating speed, remove waste liquid, water sediment fraction being added 50 ~ 60 DEG C of 2 ~ 3 times of sediment fraction weight stirs 25 ~ 35min, again with the centrifugal 10 ~ 20min of 4000 ~ 6000r/min rotating speed, remove waste liquid, get precipitation for subsequent use;
(3) enzymolysis: precipitation is added 50 ~ 55 DEG C of water and stir, obtains fish-skin slurry; Ensure that in fish-skin slurry, protein quality percentage composition is 8% ~ 15%, control constant temperature 50 ~ 52 DEG C, regulate fish-skin slurry slurry pH value to 8.0 ~ 8.5, after adding the Sumizyme MP A stirring hydrolysis 1 ~ 3h of protein quality 0.05 ~ 0.3% in fish-skin slurry, then the neutral protease B adding protein quality 0.1 ~ 0.3% in fish-skin slurry stirs hydrolysis 2 ~ 5h; Described Sumizyme MP A is Sumizyme MP Alcalase, Sumizyme MP Protex-6L or Pang Bo food grade Sumizyme MP; Described neutral protease B is neutral protease Neutrase, neutral protease Protex.7L, Pang Bo food-grade neutral protease, papoid or bromeline;
(4) to go out enzyme, decolouring: after enzymolysis terminates, be warming up to 80 ~ 85 DEG C, add activated carbon powder stirring and evenly mixing, the add-on of activated carbon powder for get described in step (2) precipitation for subsequent use in Sediment weight 2 ~ 4%, under 80 ~ 85 DEG C of constant temperatures, stir 20 ~ 40min with 30 ~ 60r/min, realize going out enzyme and adsorption bleaching one step complete;
(5) coarse filtration, essence filter: the slurries containing activated carbon after go out enzyme and adsorption bleaching are pumped into plate-frame type diatomaceous earth filter coarse filtration, the clear liquid after press filtration through the filter of plate and frame cardboard filter essence, obtains the Isin glue collagen peptide permeate of clear again;
(6) evaporation concentration: Isin glue collagen peptide permeate is concentrated into by economic benefits and social benefits falling film evaporation thickener the concentrated solution that solid quality content is 35 ~ 45%;
(7) spraying dry: concentrated solution drying tower is dry, it is 160 ~ 170 DEG C that inlet temperature controls, and it is 80 ~ 90 DEG C that temperature out controls, and dry end, namely obtains Isin glue collagen peptide.
2. preparation method according to claim 1, is characterized in that: the described fresh fish-skin of step (1) is the fish-skin of fresh tilapia after peeling is scaled of green non-pollution cultivation; It is under subzero 38 DEG C of conditions, carry out urgency freeze acid discharge that described urgency freezes acid discharge.
3. preparation method according to claim 1, is characterized in that: step (1) ~ (3) described water is deionized water.
4. preparation method according to claim 1, is characterized in that: step (2) described centrifugal be adopt separating centrifuge carry out centrifugal; The filtering accuracy of the described plate and frame cardboard filter of step (5) is 0.1um.
5. preparation method according to claim 1, is characterized in that: step (4) described activated carbon is compound activated carbon; The fineness of described activated carbon powder is 200 ~ 300 orders.
6. preparation method according to claim 1, is characterized in that: the described drying tower of step (7) adopts Centrafugal spray drying tower.
7. preparation method according to claim 1, is characterized in that: step (7) described Isin glue collagen peptide is in butt: protein quality content >=90%, and oxyproline mass content >=9%, molecular-weight average is lower than 1000Dal.
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