CN107674904A - Balsam pear active peptide, composition and preparation method - Google Patents
Balsam pear active peptide, composition and preparation method Download PDFInfo
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- CN107674904A CN107674904A CN201711068403.XA CN201711068403A CN107674904A CN 107674904 A CN107674904 A CN 107674904A CN 201711068403 A CN201711068403 A CN 201711068403A CN 107674904 A CN107674904 A CN 107674904A
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- balsam pear
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/185—Vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention provides a kind of preparation method of balsam pear active peptide and balsam pear active peptide.The preparation method comprises the following steps：A, balsam pear powder is added into water, adjust pH to 8~9, immersion 15~18 hours, separation of solid and liquid, supernatant regulation pH to 4~6 is precipitated, separation of solid and liquid, solid portion is added into water, 20~30min is heated at 85~100 DEG C, then carries out homogeneous, obtained suspension is adjusted into pH to 5~8, enzyme preparation is added and is digested；The enzyme preparation is made up of flavor protease, papain and the pepsin that weight ratio is 3~5: 1~2: 2~3, and hydrolysis temperature is 45~65 DEG C, and enzymolysis time is 2~6h, obtains enzymolysis liquid, and by enzymolysis liquid destroy the enzyme treatment, balsam pear active peptide powder is made.The preparation method simple production process of the present invention, and obtained balsam pear active peptide purity is high, content of peptides is high, good in taste.
Description
Technical field
The invention belongs to biological technical field, and in particular to a kind of preparation method of balsam pear active peptide.
Background technology
Balsam pear also known as balsam pear, it is cucurbitaceous plant.Cure mainly heatstroke, hot summer weather polydipsia, heat furuncle, prickly heat excessive, dysentery, sore swells,
The illnesss such as conjunctivitis, red eye, swell pain, carbuncle swells erysipelas, burn and scald, oliguresis.In addition, the Vitamin C content of balsam pear is very high, have pre-
Inside the effect balsam pears such as anti-scurvy, protection cell membrane, atherosclerosis, raising physical stress ability, cardioprotection
Containing a kind of composition, it can effectively suppress the canceration of normal cell and promote the special recovery for becoming cell, there is certain anticancer to make
With, and the characteristics of be also equipped with reducing blood lipid and blood pressure.Moreover, balsam pear also has pre- preventing bone rarefaction, regulation endocrine, resisted
The effect such as oxidation, while further strengthening body resistance, improve the reaction mechanism inside body.Bitter melon protein matter composition and big
Amount vitamin C can improve the immunologic function of body, make immunocyte have the function that to kill cancer cell；Bitter Melon Juice contains certain hatching egg
Bai Chengfen, macrophage ability can be strengthened, it is clinically effective to lymphosarcoma and leukaemia.
At present, it is keen to treat diabetes with balsam pear both at home and abroad, researchs and develops many similar " balsam pear teas ", " hardship in succession
The products such as melon powder ", but human body directly takes in balsam pear and is not easy to absorb and utilizes, and from theory of traditional Chinese medical science, the cold people of constitution is not
Preferably eat or should not eat more, often eat.
With the development of science and technology, the application of protein engineering, biologically active peptide and its egg are produced using zymolysis technique
The research and product development of white hydrolysate have become focus and main flow, and a kind of use is reported in patent CN201010526066.6
In prevention and/or the preparation method of the balsam pear peptide for the treatment of diabetes, fresh balsam pear (artificial growth or wild seediness balsam pear) is put into
Be freeze-dried in freeze drying equipment, be ground into powder afterwards, freeze-drying balsam pear powder.Then balsam pear powder is put into
In enzymatic vessel, the water of 9 times of balsam pear powder quality is added into enzymatic vessel, needs papain by every gram of balsam pear powder of enzymolysis
165000U amount adds papain, and balsam pear powder is digested 8 hours under the conditions of 40 DEG C of temperature.After enzymolysis terminates, to enzymolysis
Liquid carries out enzyme-deactivating processing, and the condition of enzyme-deactivating is 100 DEG C of temperature, and the time is 30 minutes.It is heavy to be refrigerated afterwards under the conditions of 2-4 DEG C
Drop processing 60 hours, treats that its impurity precipitates substantially, then is filtered three times with filter plant, produces bitter gourd polypeptide concentrate.By the hardship
Melon polypeptide concentrate is freeze-dried or spray drying treatment, that is, obtains bitter gourd polypeptide powder.
First protein in balsam pear is not purified out in the method, be unfavorable for obtaining the bitter gourd polypeptide powder of high-purity, and led
Cause impurity excessive, be extremely worthless for industrial production so being settled at low temperature in patent 60 hours.Other enzyme dosage
It is excessive, but enzymolysis efficiency is relatively low, and enzymolysis time is also long, and energy consumption wastes big, cost height.
The content of the invention
An object of the present disclosure is to provide a kind of preparation method of balsam pear active peptide, and this method technique is simple, and is made
Balsam pear active peptide content of peptides it is high.
Another object of the present invention is to provide a kind of composition containing balsam pear active peptide.
It is still another object of the present invention to provide the preparation method of the composition containing balsam pear active peptide.
The preparation method of the balsam pear active peptide of the present invention, comprises the following steps：
A, balsam pear powder is added into water, adjusts pH to 8~9, soaked 15~18 hours, separation of solid and liquid, take supernatant；
B, supernatant regulation pH to 4~6 is precipitated, separation of solid and liquid, takes solid portion；
C, the solid portion is added into water, 20~30min is heated at 85~100 DEG C, homogeneous is then carried out, is mixed
Suspension；
D, the suspension is adjusted into pH to 5~8, adds enzyme preparation and digested；The enzyme preparation by flavor protease,
Papain and pepsin composition, and the weight ratio of the flavor protease, papain and pepsin is 3~5:
1~2: 2~3, hydrolysis temperature is 45~65 DEG C, and enzymolysis time is 2~6h, obtains enzymolysis liquid；
E, by the enzymolysis liquid destroy the enzyme treatment, balsam pear active peptide powder is then made.
Preparation in accordance with the present invention, it is preferable that in step B, the supernatant is adjusted by pH to 4~6 using weak acid；
The weak acid is selected from least one of acetic acid, lactic acid, citric acid.
Preparation in accordance with the present invention, it is preferable that in step E, the temperature of the destroy the enzyme treatment is 95~125 DEG C, when
Between be 0.5~2h.
Preparation in accordance with the present invention, it is preferable that in step E, the enzymolysis liquid after the destroy the enzyme treatment is concentrated, homogeneous
Emulsification, is then spray-dried, obtains balsam pear active peptide powder；The spray drying is carried out at 80~105 DEG C.
Preparation in accordance with the present invention, it is preferable that in step E, the enzymolysis liquid after the destroy the enzyme treatment is centrifuged,
Centrifugate filters through plate and frame filter press first, then through ceramic membrane filter, obtains active peptide permeate；The active peptide filtrate is dense
Contracting, spray drying, so as to obtain balsam pear active peptide powder；The spray drying is carried out at 80~105 DEG C；The plate and frame filter press
It is interior containing combination filtration adjuvant, it is described combination filtration adjuvant by activated carbon, perlite and diatomite according to weight ratio 3~5: 2~4
: 1~2 composition.
The present invention also provides a kind of balsam pear active peptide, and the balsam pear active peptide is prepared using the above method.
The preparation method enzymolysis efficiency of the balsam pear active peptide of the present invention is higher, and cost is lower；And currently preferred implementation
In mode, the balsam pear active peptide of acquisition is advantageous to consumer's receiving without bitter taste.
The present invention also provides a kind of composition of active peptide containing balsam pear, and it is by the parts by weight of balsam pear active peptide 70~85, bitter buckwheat 5
~15 parts by weight and the parts by weight of coconut milk powder 10~15 are made.
The present invention also provides a kind of preparation method of the composition of the active peptide containing balsam pear, and the preparation method includes：
(1) balsam pear active peptide powder is prepared using the above method；(2) bitter buckwheat and coconut milk are prepared as buckwheat powder and coconut milk powder respectively；
(3) the balsam pear active peptide powder, buckwheat powder and coconut milk powder are mixed in proportion, obtains the composition.
The present invention also provides the composition of another active peptide containing balsam pear, its by the parts by weight of balsam pear powder 90~98, bitter buckwheat 1~
5 parts by weight and the parts by weight of coconut milk powder 1~5 are made.
The present invention also provides the preparation method of the composition of the active peptide containing balsam pear, including：
A ', balsam pear powder is added to water, adjust pH to 8~9, soaked 15~18 hours, separation of solid and liquid, take supernatant；
B ', by the supernatant regulation pH to 4~6 precipitated, separation of solid and liquid, take solid portion；
C ', by the solid portion add water, at 85~100 DEG C heat 20~30min, then carry out homogeneous, obtain
Suspension；
D ', the suspension is adjusted to pH to 5~8, addition enzyme preparation is digested；The enzyme preparation is by flavor albumen
Enzyme, papain and pepsin composition, and the weight ratio of the flavor protease, papain and pepsin is 3
~5: 1~2: 2~3；Hydrolysis temperature is 45-65 DEG C, enzymolysis time 2-6h, obtains enzymolysis liquid；
D1 ', the bitter buckwheat and coconut milk powder added in the enzymolysis liquid, heating and refluxing extraction, filtering, mixed
Liquid；
E ', the composition that the mixed liquor is made to the active peptide containing balsam pear.
The preparation method of the balsam pear active peptide of the present invention, specific Chinese traditional medicine composition bitter buckwheat and coconut milk powder, can enter one first
The blood sugar decreasing effect of balsam pear active peptide prepared by step lifting.Bitter buckwheat and coconut milk powder are as follows to the main function of body：
Bitter buckwheat is medicine-food two-purpose crop very few in nature, according to《Compendium of Materia Medica》Record：Bitter buckwheat bitter, cold, energy
Real stomach, benefiting energy, continue spirit, sharp knowledge, it is dirty to refine the five internal organs slag；《Qianjin Yao Fang》、《Dictionary of medicinal plant》And in pertinent literature
It is all on the books to bitter buckwheat：Can calm the nerves, lively atmosphere blood, the wide intestines of sending down abnormally ascending, heat-clearing swell wind pain, productization of dispelling is stagnant, gut purge, ease constipation, defaecation, stops
Cough, relieving asthma, anti-inflammatory, antiallergy, cardiac stimulant, fat-reducing, beauty and other effects.Coconut milk powder, coconut juice contain the mineral such as abundant potassium, magnesium
Matter, its composition is similar to intracellular fluid, can correct dehydration and electrolyte disturbance, reach the effect of inducing diuresis to remove edema；Coconut meat and coconut juice are equal
Play the role of to kill Enterozoa, fasciolopsis and tapeworm can be driven away by drinking its juice, be used for clinic, and not only curative effect is reliable, and nothing
Toxic side effect, it is preferable destroying parasites for curing malnutrition food；Other coconut juice is containing carbohydrate, fat, protein, growth hormone, vitamin and big
The essential trace elements of the human body of amount, often drinks, the beneficial people's strength of energy, supplement intracellular fluid, expanding blood volume, skin care,
Acted on conditioning skin and losing.
In addition to objects, features and advantages described above, the present invention also has other objects, features and advantages.
The present invention is further detailed explanation below.
Embodiment
With reference to specific embodiment, the present invention is further illustrated, but protection scope of the present invention is not limited to
This.
<The preparation method and balsam pear active peptide of balsam pear active peptide>
The preparation method of the balsam pear active peptide of the present invention, comprises the following steps：
A, balsam pear powder is added into water, adjusts pH to 8~9, soaked 15~18 hours, separation of solid and liquid, take supernatant；
B, supernatant regulation pH to 4~6 is precipitated, separation of solid and liquid, takes solid portion；
C, the solid portion is added into water, 20~30min is heated at 85~100 DEG C, homogeneous is then carried out, is mixed
Suspension；
D, the suspension is adjusted into pH to 5~8, adds enzyme preparation and digested；The enzyme preparation by flavor protease,
Papain and pepsin enzyme composition, and the weight ratio of the flavor protease, papain and pepsin be 3~
5: 1~2: 2~3；Hydrolysis temperature is 45~65 DEG C, and enzymolysis time is 2~6h, obtains enzymolysis liquid；
E, by the enzymolysis liquid destroy the enzyme treatment, balsam pear active peptide powder is then made.
Preparation in accordance with the present invention, in step A, the particle diameter of the balsam pear powder is 5~100 μm.The balsam pear powder can
So that by the way that dry balsam pear is carried out into ultramicro grinding, then sieving obtains, such as crosses 200~600 mesh sieves.Using particle diameter of the invention
Balsam pear powder, enzymatic hydrolyzation can be effectively improved in the enzymolysis step in later stage, make full use of the protein ingredient in balsam pear.The immersion
Preferably soaked under agitation, so that the composition in balsam pear powder is fully dissolved out.In step A, amount of water is balsam pear powder weight
3~8 times, preferably 3~5 times of amount.Preferably, after the completion of process is soaked, mechanical agitation is carried out, so that liquid is uniform,
It is easy to centrifuge.
In step A, the solid-liquid separating method can be filtering, centrifugation etc., and be preferably to centrifuge.In step A, leaching
Bubble process can be heated or carried out at normal temperatures, and preferably be carried out at normal temperatures.Generally, need to be heated during dipping by lye
Processing；And inventor has found, the composition in balsam pear powder can be fully dissolved out by immersion at normal temperatures, so as to avoid carrying out heating production
Raw additional energy, and more convenient operation.
Preparation in accordance with the present invention, in step B, the supernatant is adjusted by pH to 4~6 using weak acid.It is described weak
Acid can be selected from least one of acetic acid, lactic acid, citric acid.In existing balsam pear active peptide extracting method, it is contemplated that regulation
Efficiency, the step for the strong acid for adjusting pH value of generally use to acidity, the strong acid is, for example, hydrochloric acid, sulfuric acid etc., and less consideration
Influence of the property of acid for product；Inventor has found that strong acid is larger for protein component destruction, to the balsam pear finally given
Active peptide Product Activity has a negative impact.And weak acid for adjusting pH is used, protein precipitation is come out, reduces and protein is sought
The destruction of material is supported, and the balsam pear active peptide Product Activity finally given is more preferable.In step B, it is preferable that the sedimentation time is
8~10h, so as to ensure that the protein component in supernatant is fully precipitated out under above-mentioned pH.Preferably, process is precipitated to complete
Mechanical agitation is carried out afterwards to carry out under agitation, so that liquid is uniform, is easy to centrifuge.
Preparation in accordance with the present invention, in step C, make protein denaturation using heating means.In described fire-bar
It can realize that the protein component in balsam pear is denatured completely under part, and be advantageous to fully be digested in subsequent step, simultaneously
And avoid pollution of the chemical reagent to product.Homogeneous is carried out after heat distortion, further increases enzymolysis efficiency.
Preparation in accordance with the present invention, in step D, the suspension is adjusted to pH5~8, preferably pH5.5~
7.5, more preferably pH6~7.In the present invention, pH is adjusted to the enzyme preparation progress enzyme for above-mentioned alkalescent, being more beneficial for the present invention
Solution.In the present invention, enzyme preparation is 3~5: 1~2: 2~3 according to weight ratio by flavor protease, papain and pepsin
Composition.According to the preferred embodiment of the present invention, the weight ratio of flavor protease, papain and pepsin for 3.3~
4.8: 1.3~1.8: 2~2.5.According to one preferred embodiment of the invention, flavor protease, papain and stomach egg
The weight ratio of white enzyme is 3.9: 1.5: 2.3.The enzymolysis process of the present invention disposably adds above-mentioned enzyme preparation, and in above-mentioned pH conditions
Under digested, more preferably hydrolysis result can be obtained.The enzyme preparation of the present invention is imitated for the enzymolysis of the protein component in balsam pear
Rate is high, hence it is evident that and it is alone higher than every kind of enzyme, and it is higher than existing balsam pear active peptide enzyme.When flavor protease, papain and
When the weight ratio of pepsin is 3.9: 1.5: 2.3, enzymolysis efficiency highest.Preparation in accordance with the present invention, the enzyme preparation
Addition can be the suspension in solid content weight 0.1~2wt%, preferably 0.3~1.5wt%, more preferably
0.4~1.2wt%.In the present invention, hydrolysis temperature is 45~65 DEG C, and enzymolysis time is 2~6h；Preferably, hydrolysis temperature 45
~60 DEG C, enzymolysis time is 3~5h.The addition of above-mentioned enzyme preparation, enzymolysis time and at a temperature of, bitter melon protein can have been realized
Effect enzymolysis.
Numerous studies have been done in the selection that applicant is directed to the complex enzyme for being used for digesting balsam pear active peptide, are found using the present invention
Complex enzyme to carry out hydrolysis result optimal, and be substantially better than the complex enzyme of other species and consumption proportion.
Preparation in accordance with the present invention, in step E, the temperature of the destroy the enzyme treatment is 95~125 DEG C, the time 0.5
~2h.In step E, the enzymolysis liquid after the destroy the enzyme treatment is centrifuged, centrifugate filters through plate and frame filter press first, then passes through
Ceramic membrane filter, obtain active peptide permeate；The active peptide filtrate is concentrated, spray drying, so as to obtain balsam pear active peptide
Powder.
In the present invention, containing combination filtration adjuvant in the plate and frame filter press, the combination filtration adjuvant is by activated carbon, treasure
Zhu Yan and diatomite composition, and activated carbon, perlite and diatomaceous weight ratio 3~5: 2~4: 1~2.According to the one of the present invention
Individual preferred embodiment, it is described to combine activated carbon, perlite and diatomaceous weight ratio 4: 3.5: 2 in filtration adjuvant.According to
Another preferred embodiment of the present invention, it is described to combine activated carbon, perlite and diatomaceous weight ratio in filtration adjuvant
4.2∶3.6∶1.8.Inventor has found that balsam pear active peptide prepared by existing method carries bitter taste, poor taste；A variety of filtration adjuvant lists
With being difficult to remove.The present invention filtration adjuvant can effectively remove balsam pear per se with bitter taste.In the present invention, the ceramics
The aperture of film can be 20~500nm, more preferably 20~300mm.Using such ceramic membrane aperture, enzymolysis liquid can be realized
Further refine.
After filtration treatment by the present invention, peptide content >=90wt% in obtained balsam pear active peptide powder.
Generally, the spray-drying operation of peptide is in 120~140 DEG C of progress, it is considered that such drying temperature is for peptide
Activity influence is little.However, it is found by the inventors that the temperature of existing spray drying significantly affects the activity of peptide.And in the present invention,
Preferably, the low-temperature spray drying tower in, the spray-drying operation are carried out at 80~105 DEG C, so as to effectively keep balsam pear
The activity of active peptide.
The balsam pear active peptide of the present invention has good blood sugar decreasing effect, and enzymolysis efficiency is higher, and production technology is more excellent.
<Composition of the active peptide containing balsam pear and preparation method thereof>
The composition of the active peptide containing balsam pear of the present invention can be by the parts by weight of balsam pear active peptide 70~85, the weight of bitter buckwheat 5~15
Amount part and the parts by weight of coconut milk powder 10~15 are made.
The preparation method of the composition of the active peptide containing balsam pear of the present invention, can be obtained using a variety of preparation methods.
According to an embodiment of the invention, the preparation method of the composition is：(1) balsam pear active peptide powder is prepared；
(2) bitter buckwheat and coconut milk powder are prepared as buckwheat powder and coconut milk powder respectively；(3) by the balsam pear active peptide powder, buckwheat powder and
Coconut milk powder is well mixed, and obtains the composition of the present invention.The balsam pear active peptide powder preferably uses above-mentioned preparation method system
It is standby to obtain.The buckwheat powder is preferably that bitter buckwheat progress ultramicro grinding is made.Wherein, step (1 ') is using above-mentioned " balsam pear activity
The preparation method of peptide " is made, and repeats no more here.
The composition of the active peptide containing balsam pear of the present invention can also be by the parts by weight of balsam pear powder 90~98, the parts by weight of bitter buckwheat 1~5
It is made with the parts by weight of coconut milk powder 1~5.
Preferably, the preparation method of the composition of the peptide containing balsam pear is：
A ', balsam pear powder is added to water, adjust pH to 8~9, soaked 15~18 hours, separation of solid and liquid, take supernatant；
B ', by the supernatant regulation pH to 4~6 precipitated, separation of solid and liquid, take solid portion；
C ', by the solid portion add water, at 85~100 DEG C heat 20~30min, then carry out homogeneous, obtain
Suspension；
D ', the suspension is adjusted to pH to 5~8, addition enzyme preparation is digested；The enzyme preparation is by flavor albumen
Enzyme, papain and pepsin composition, and the weight ratio of the flavor protease, papain and pepsin is 3
~5: 1~2: 2~3；Hydrolysis temperature is 45-65 DEG C, enzymolysis time 2-6h, obtains enzymolysis liquid；
D1 ', the bitter buckwheat and coconut milk powder added in the enzymolysis liquid, heating and refluxing extraction, filtering, mixed
Liquid；
E ', the composition that the mixed liquor is made to the active peptide containing balsam pear.
Wherein, step A '~D ' concrete operations and parameter respectively with A~D in above-mentioned " preparation method of balsam pear active peptide "
It is identical, repeat no more here.
In step D1 ', it is preferable that the bitter buckwheat and coconut milk add in the enzymolysis liquid in powder form, so as to improve
Recovery rate.The heating and refluxing extraction time is 1~3 hour, preferably 1~2 hour.The filtering can use customary filtration methods,
So as to filter off the dregs of a decoction, mixed liquor is obtained.
According to one preferred embodiment of the invention, in step E ', the mixed liquor is centrifuged, centrifugate is first
Filtered through plate and frame filter press, then through ceramic membrane filter, filtrate concentration, spray drying, so as to obtain refined active peptide containing balsam pear
Composition.Preferably, the spray-drying operation is in 80~105 DEG C of progress, so as to effectively keep the activity of balsam pear active peptide.
It is described to combine filtration adjuvant as described above containing combination filtration adjuvant in the plate and frame filter press in the present invention,
Here repeat no more.The present invention filtration adjuvant except remove balsam pear per se with peculiar smell and bitter taste, remove grease-like composition,
It also removes the non-solubility composition in the bitter buckwheat and coconut milk powder in mixed liquor.In the present invention, the aperture of the ceramic membrane can
Think 20~500nm, more preferably 20~300mm.Using such ceramic membrane aperture, the further essence of mixed liquor can be realized
System.Preferably, the spray-drying operation is in 80~105 DEG C of progress, so as to effectively keep the activity of balsam pear active peptide.
After filtration treatment by the present invention, the composition of the refined active peptide containing balsam pear, peptide content >=90wt% are obtained.
The composition of the active peptide containing balsam pear of the present invention has more preferably blood sugar decreasing effect.
Embodiments of the invention are described in detail below, but protection scope of the present invention is not limited.
Embodiment 1
The preparation method of balsam pear active peptide, comprises the following steps：
A, dry balsam pear is subjected to ultramicro grinding, crosses 400 mesh sieves, obtain balsam pear powder；Balsam pear powder is added into balsam pear powder weight 4.5
The water of amount again, mixing, adjusted with sodium hydroxide solution to pH8, soak 16h；Mechanical agitation, centrifugation, takes supernatant；
B, the supernatant is adjusted to pH5, precipitating proteins, mechanical agitation with acetum, centrifugation, takes solid part
Point；
C, the solid portion is added into water, 30min is heated at 90 DEG C, homogeneous is then carried out, obtains suspension；
D, the suspension is adjusted into pH to 8, adds enzyme preparation and digested, obtain enzymolysis liquid；The enzyme preparation is by flavor
Protease, papain and pepsin form according to weight ratio 3.9: 1.5: 2.3；The dosage of enzyme preparation is solid in suspension
The 0.6wt% of shape thing weight；Hydrolysis temperature is 50 DEG C, enzymolysis time 3h；
E, the enzymolysis liquid is subjected to destroy the enzyme treatment, enzyme-removal temperature is 90 DEG C, time 1h；By enzymolysis solution after enzyme deactivation from
The heart, centrifugate filter through plate and frame filter press, contain filtration adjuvant in plate and frame filter press, filtration adjuvant by activated carbon, perlite and
Diatomite is according to weight than 4: 3.5: 2 compositions；Via hole diameter is 400nm ceramic membrane filter again, obtains active peptide permeate；Should
Active peptide permeate concentrates, and in 85 DEG C of spray drying in middle low-temperature spray drying tower, obtains balsam pear active peptide powder.
Embodiment 2
Buckwheat powder and coconut milk powder is made in bitter buckwheat and coconut milk powder respectively.By the weight of balsam pear active peptide 80 of embodiment 1
12 parts of part, the parts by weight of buckwheat powder 8 and coconut milk powder are well mixed, obtain the composition of the active peptide containing balsam pear.
Embodiment 3
The preparation method of balsam pear active peptide composition, comprises the following steps：
A, by dry balsam pear carry out ultramicro grinding, cross 400 mesh sieves, obtain balsam pear powder；The parts by weight of balsam pear powder 96 are added into balsam pear
The water of 5 times of amounts of powder weight, mixing, adjusted with sodium hydroxide solution to pH8, soak 17h；Mechanical agitation, centrifugation, takes supernatant；
B ', the supernatant adjusted to pH5.5, precipitating proteins, mechanical agitation with acetum, centrifugation, take solid
Part；
C ', by the solid portion add water, 25min is heated at 95 DEG C, homogeneous is then carried out, obtains suspension；
D ', the suspension is adjusted to pH to 7.5, addition enzyme preparation is digested, and obtains enzymolysis liquid；The enzyme preparation is by wind
Taste protease, papain and pepsin form according to weight ratio 3.7: 1.4: 2.1；The dosage of enzyme preparation is in suspension
The 0.6wt% of solid content weight；Hydrolysis temperature is 50 DEG C, enzymolysis time 4h；
D1 ', the parts by weight of bitter buckwheat 3 and the parts by weight of coconut milk powder 3 are added in the enzymolysis liquid, heating and refluxing extraction,
Extraction time is 1.5h, filtering, obtains mixed liquor；
E ', the enzymolysis liquid is carried out to destroy the enzyme treatment, enzyme-removal temperature is 95 DEG C, time 50min；By enzymolysis solution after enzyme deactivation
Centrifugation, centrifugate filter through plate and frame filter press, filtration adjuvant are contained in plate and frame filter press, filtration adjuvant is by activated carbon, perlite
Formed with diatomite according to weight ratio 4.2: 3.6: 1.8；Via hole diameter is 400nm ceramic membrane filter again, obtains active peptide transmission
Liquid；The active peptide permeate is concentrated, in 85 DEG C of spray drying in middle low-temperature spray drying tower, obtains the combination of balsam pear active peptide
Thing.
Comparative example 1
Except by the step D of embodiment 1 enzyme preparation replace with by flavor protease, papain and pepsin by
It is same as Example 1 according to 2: 3: 4 compositions, other steps.
Comparative example 2
Except the step B of embodiment 1 acetum is replaced with into hydrochloric acid solution, other steps are same as Example 1.
Comparative example 3
Activated carbon except the step E of embodiment 1 filtration adjuvant to be replaced with to equivalent weight, other steps and embodiment
1 is identical.
Comparative example 4
Perlite except the step E of embodiment 1 filtration adjuvant to be replaced with to equivalent weight, other steps and embodiment
1 is identical.
Comparative example 5
Except step E in embodiment 1 without filter operation, but step E enzymolysis solution after enzyme deactivation is directly entered
Row concentration, in 85 DEG C of spray drying in middle low-temperature spray drying tower, balsam pear active peptide is obtained, other steps and the phase of embodiment 1
Together.
Comparative example 6
Bitter gourd polypeptide is prepared according to the method for patent document CN201010526066.6 embodiments 1.
Determine peptide content and all embodiments and comparative example activity in the balsam pear active peptide of embodiment 1 and comparative example 1~6
The taste of peptide, the results are shown in Table 1.
The content assaying method of peptide is：Carried out according to the assay method of peptide content in the soy peptide powders of GB/T 22492.
Bitter taste estimate method is：Take 1g balsam pears active peptide to add in 10ml warm water to stir, extension rate is calculated as hardship
Taste value, 0- is without bitter taste；1- slightly has bitter taste；2- bitter tastes are weaker；3- has bitter taste；4- bitter tastes are general；5- bitter tastes are laid particular stress on；6- bitter tastes compared with
Weight；7- bitter tastes are very heavy, calculate the average bitterness value of 20 people.
Table 1
The present invention is not limited to above-mentioned embodiment, in the case of without departing substantially from the substantive content of the present invention, this area skill
Any deformation, improvement, the replacement that art personnel are contemplated that each fall within the scope of the present invention.
Claims (9)
1. a kind of preparation method of balsam pear active peptide, it comprises the following steps：
A, balsam pear powder is added into water, adjusts pH to 8~9, soaked 15~18 hours, separation of solid and liquid, take supernatant；
B, supernatant regulation pH to 4~6 is precipitated, separation of solid and liquid, takes solid portion；
C, the solid portion is added into water, 20~30min is heated at 85~100 DEG C, homogeneous is then carried out, is suspended
Liquid；
D, the suspension is adjusted into pH to 5~8, adds enzyme preparation and digested；The enzyme preparation is by flavor protease, pawpaw
Protease and pepsin composition, and the weight ratio of the flavor protease, papain and pepsin is 3~5: 1~2
: 2~3, hydrolysis temperature is 45~65 DEG C, and enzymolysis time is 2~6h, obtains enzymolysis liquid；
E, by the enzymolysis liquid destroy the enzyme treatment, balsam pear active peptide powder is then made.
2. preparation method according to claim 1, it is characterised in that in step B, adjusted the supernatant using weak acid
PH to 4~6；The weak acid is selected from least one of acetic acid, lactic acid, citric acid.
3. preparation method according to claim 1, it is characterised in that in step E, the temperature of the destroy the enzyme treatment for 95~
125 DEG C, the time is 0.5~2h.
4. the preparation method of balsam pear active peptide according to claim 1, it is characterised in that in step E, at the enzyme deactivation
Enzymolysis liquid after reason is centrifuged, and centrifugate filters through plate and frame filter press first, then through ceramic membrane filter, obtains active peptide transmission
Liquid；The active peptide filtrate is concentrated, spray drying, so as to obtain balsam pear active peptide powder；The spray drying is at 80~105 DEG C
Lower progress；Containing combination filtration adjuvant in the plate and frame filter press, the combination filtration adjuvant is by activated carbon, perlite and diatom
Soil is according to weight than 3~5: 2~4: 1~2 compositions.
5. a kind of balsam pear active peptide, it is characterised in that the balsam pear active peptide is used according to any one of Claims 1 to 5
Method be prepared.
A kind of 6. composition of active peptide containing balsam pear, it is characterised in that the composition by the parts by weight of balsam pear active peptide 70~85,
The parts by weight of bitter buckwheat 5~15 and the parts by weight of coconut milk powder 10~15 are made.
7. the preparation method of the composition of the active peptide according to claim 6 containing balsam pear, it is characterised in that the preparation side
Method includes：(1) method according to any one of Claims 1 to 5 prepares balsam pear active peptide powder；(2) respectively by bitter buckwheat and coconut palm
Sub- juice powder is prepared as buckwheat powder and coconut milk powder；(3) the balsam pear active peptide powder, buckwheat powder and coconut milk powder are well mixed,
Obtain the composition.
8. the composition of a kind of active peptide containing balsam pear, it is characterised in that the composition is by the parts by weight of balsam pear powder 90~98, bitter buckwheat
1~5 parts by weight and the parts by weight of coconut milk powder 1~5 are made.
9. the preparation method of the composition of the active peptide according to claim 8 containing balsam pear, it is characterised in that the preparation side
Method comprises the following steps：
A ', balsam pear powder is added to water, adjust pH to 8~9, soaked 15~18 hours, separation of solid and liquid, take supernatant；
B ', by the supernatant regulation pH to 4~6 precipitated, separation of solid and liquid, take solid portion；
C ', by the solid portion add water, at 85~100 DEG C heat 20~30min, then carry out homogeneous, be suspended
Liquid；
D ', the suspension is adjusted to pH to 5~8, addition enzyme preparation is digested；The enzyme preparation is by flavor protease, wood
Melon protease and pepsin composition, and the weight ratio of the flavor protease, papain and pepsin is 3~5: 1
~2: 2~3；Hydrolysis temperature is 45-65 DEG C, enzymolysis time 2-6h, obtains enzymolysis liquid；
D1 ', the bitter buckwheat and coconut milk powder added in the enzymolysis liquid, heating and refluxing extraction, filtering, obtain mixed liquor；
E ', the composition that the mixed liquor is made to the active peptide containing balsam pear.
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| CN111850074A (en) * | 2020-07-10 | 2020-10-30 | 陕西慧科植物开发有限公司 | Preparation method and application of bitter gourd polypeptide |
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Application publication date: 20180209 |