CN112704232A - Balsam pear seed extract for scavenging free radicals and preparation method and application thereof - Google Patents
Balsam pear seed extract for scavenging free radicals and preparation method and application thereof Download PDFInfo
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- CN112704232A CN112704232A CN202011610805.XA CN202011610805A CN112704232A CN 112704232 A CN112704232 A CN 112704232A CN 202011610805 A CN202011610805 A CN 202011610805A CN 112704232 A CN112704232 A CN 112704232A
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/18—Antioxidants, e.g. antiradicals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
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- Natural Medicines & Medicinal Plants (AREA)
- Engineering & Computer Science (AREA)
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- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- Dermatology (AREA)
- Organic Chemistry (AREA)
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- Polymers & Plastics (AREA)
- Birds (AREA)
- Food Science & Technology (AREA)
- Gerontology & Geriatric Medicine (AREA)
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- Alternative & Traditional Medicine (AREA)
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- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to a balsam pear seed extract for scavenging free radicals, a preparation method and application thereof, wherein the total sugar content in the balsam pear seed extract is not less than 10wt%, the peptide content is 55-65 wt%, and the polyphenol and flavone content is more than 5 wt%. The invention obtains the balsam pear seed extract after crushing, enzyme extraction, filtration, concentration and drying of the balsam pear seed meal. The preparation process does not use any organic solvent and toxic and harmful compounds, has simple and convenient operation, safety, high yield, low cost and environmental protection, and is suitable for large-scale production.
Description
Technical Field
The invention relates to the technical field of bitter gourd seed extracts, in particular to a bitter gourd seed extract which can be used for removing free radicals and is rich in chemical components such as saccharides, peptides, phenols and flavonoids which are beneficial to human bodies. The invention also relates to a preparation method of the balsam pear seed extract, which is prepared by enzyme extraction, filtration, concentration and drying, and the extract can be used as food, health food, medicine or cosmetics.
Background
Balsam pear is a beneficial medicinal plant and vegetable, the fruit is bitter in taste, and is commonly used for cooking. The bitter gourd is already listed in the traditional Chinese medicine category in ancient China, is bitter in smell, non-toxic and cold in nature, and has the effects of clearing away summer heat, improving eyesight, detoxifying, promoting urination, cooling blood, relieving fatigue, clearing away the heart-fire, tonifying qi and strengthening yang. The balsam pear has good nutrition and medicinal value, has obvious effect on clinical treatment of diabetes, inflammatory reaction and cancer, and has been used as a functional food with homology of medicine and food for a long time in China, Japan and other countries. The balsam pear seeds have great similarity to the balsam pear in the aspects of nutrition and medicinal value. The balsam pear seeds contain 19.5 percent of fiber, 16.4 percent of carbohydrate, 31.0 percent of grease and 36.5 percent of protein. In addition, the balsam pear seeds also contain 2.71 percent of flavone and a plurality of trace elements necessary for human bodies, wherein the K, Mg and P contents are the highest, and the Cr and Zn contents are respectively as high as 5.165 mug/g and 45.45 mug/g.
At present, the most common methods for obtaining the balsam pear seed extract are a water extraction method and an organic solvent extraction method, and comprise water extraction, or alcohol extraction after water extraction, or alcohol extraction after alcohol extraction, or supercritical carbon dioxide extraction and other methods, and the defects are that:
1. whether water extraction, alcohol extraction or alcohol-water mixed extraction, or supercritical carbon dioxide extraction, or other organic solvent extraction is adopted, the protein component in the balsam pear seed medicinal material cannot be completely extracted;
2. the extraction is carried out by single water extraction, alcohol extraction or mixed extraction of alcohol and water, and high temperature extraction is needed for many times for improving the yield, but the yield is not ideal, and the resource utilization rate is low, so that the production requirement of environmental protection cannot be met;
3. the supercritical carbon dioxide extraction or other organic solvent extraction is adopted, the cost is high, and the use of other organic solvents can not meet the requirements of environmental protection and safety and no toxicity to human bodies.
In summary, the existing extraction methods of water and organic solvents extract polysaccharide and flavonoid components in the bitter gourd seeds, but the protein components are difficult to extract completely, and the two methods need high-temperature multiple extraction, the yield is not high, the resource utilization rate is low, so that the cost is high, and the bioactivity of the obtained bitter gourd seed extract is not high.
Disclosure of Invention
In order to obtain the bitter gourd extract with high biological activity, the invention aims to provide the bitter gourd seed extract for scavenging free radicals.
In order to obtain the bitter gourd seed extract with low cost and suitable for industrialized large-scale method, the invention also aims to provide the bitter gourd seed extract for scavenging free radicals and a preparation method thereof.
The invention also aims to provide application of the balsam pear seed extract.
The invention provides the following technical scheme:
a bitter gourd seed extract for scavenging free radicals is characterized in that the total sugar content of the bitter gourd seed extract is not less than 10wt%, the peptide content is 55-65 wt%, the polyphenol content is more than 5wt%, and the flavone content is more than 5 wt%.
The method for measuring the total sugar content is a phenol-sulfuric acid method, the method for detecting the peptide content refers to the peptide content measuring method described in GB/T22492-2008 appendix B and GB/T22729-2008, the method for measuring the polyphenol content is a Folin phenol measuring method, and the method for measuring the flavone content is an aluminum nitrate color development method.
As a preferred method of the invention, the preparation method comprises the following steps: mixing the bitter gourd seed meal with water or alcohol, performing enzyme extraction, concentrating, and drying to obtain the bitter gourd seed extract.
Preferably, the extract is concentrated to a relative density of 1.0 to 1.2g/mL at 30 ℃, and dried to obtain the balsam pear seed extract.
In the present invention, the relative density is preferably 1.0 to 1.1g/mL, or 1.1 to 1.15g/mL, or 1.15 to 1.2 g/mL.
Preferably, the bitter gourd seed meal is derived from the bitter gourd seed meal degreased by hot pressing or cold pressing or a supercritical fluid extraction method.
The preparation method of the balsam pear seed extract comprises the following steps: adding water or alcohol into the bitter gourd seed meal, stirring, performing enzymolysis extraction, concentrating and drying to obtain a bitter gourd seed extract, and the steps are as follows:
(1) crushing the bitter gourd seed meal, and sieving the crushed bitter gourd seed meal with a sieve of more than 40 meshes to obtain bitter gourd seed powder;
(2) mixing a certain amount of bitter gourd seed powder with water or alcohol according to a mass ratio of 1: 10-1: 20, adding biological enzyme which is 0.1-0.5% of the mass of the bitter gourd seed powder, adjusting the pH value to 2-10, and stirring at a constant temperature of 35-60 ℃ for at least 2 hours;
(3) after extraction is finished, boiling the extracting solution in the step (2) for 5-10 min, cooling to room temperature, and filtering to obtain filtrate for later use;
(4) and (4) concentrating the filtrate obtained in the step (3) to a relative density of 1.0-1.2 g/mL at 30-80 ℃, and drying to obtain the bitter gourd seed extract.
The inventor successfully researches a biological enzyme extraction method through a plurality of tests. The method adds a small amount of biological enzyme into the extracting solution to improve the extracting efficiency, and the yield of the balsam pear seed extract is not lower than 25 wt%. Meanwhile, the protein in the balsam pear seed meal can be converted into peptide substances which are well absorbed and utilized by human bodies, the extraction temperature is mild, and the extraction times are only once, so that the resource utilization rate is greatly improved, the production cost is reduced, and the method is green, environment-friendly and suitable for large-scale production.
The filtration may be membrane filtration, centrifugal filtration and ordinary filtration (e.g., plate-and-frame filtration, screen filtration), and preferably membrane filtration and centrifugal filtration are used. The concentration method can be vacuum concentration, atmospheric concentration, membrane concentration, and preferably vacuum concentration. The drying method can be vacuum drying, heating drying, airing, air drying, freeze drying and spray drying.
As the optimization of the method, the biological enzyme used for the biological enzyme enzymolysis can be one of neutral protease with the enzyme activity of more than or equal to 30 ten thousand u/g, papain with the enzyme activity of more than or equal to 40 ten thousand u/g, bromelain with the enzyme activity of more than or equal to 30 ten thousand u/g, alkaline protease with the enzyme activity of more than or equal to 20 ten thousand u/g, acid protease with the enzyme activity of more than or equal to 20 ten thousand u/g, pepsin with the enzyme activity of more than or equal to 50 ten thousand u/g, pancreatin with the enzyme activity of more than or equal to 3000u/g, ficin with the enzyme activity of more than or equal to 5 ten thousand u/g, and momordica grosvenori protease with the enzyme activity of more than.
A composition comprising an extract of seeds of bitter gourd, said composition being a pharmaceutically or dietetically acceptable adjuvant;
the formulation of the auxiliary agent is selected from plain tablets, film-coated tablets, sugar-coated tablets, enteric-coated tablets, dispersible tablets, capsules, granules, oral solution or oral suspension, and cosmetic formulation liquid, emulsion, cream, powder or blocks.
The extract of semen Momordicae Charantiae can be used for preparing food, health food, medicine or cosmetic for preventing or treating symptoms caused by excessive free radicals.
The composition can be used for preparing food, health food, medicine or cosmetic for preventing or treating symptoms caused by excessive free radicals.
The invention has the following beneficial effects:
compared with the existing water extraction method, organic solvent method or carbon dioxide supercritical extraction method, the method has the following advantages:
1. the invention uses biological enzyme for extraction, the extraction temperature is mild, the extraction frequency is only once, and the yield is higher than that of the prior art, thereby improving the resource utilization rate and reducing the production cost;
2. the preparation process of the invention does not use any organic solvent or toxic and harmful chemical substances, is safe, has no toxic substance residue, is harmless to human body, and is green and environment-friendly;
3. the invention filters the extracting solution through a membrane, reduces the residue of the dregs of a decoction and improves the clarity of the aqueous solution.
Detailed Description
The following further describes the embodiments of the present invention.
The starting materials used in the present invention are commercially available or commonly used in the art, unless otherwise specified, and the methods in the following examples are conventional in the art, unless otherwise specified.
Preparation of example 1
Pulverizing bitter gourd seed meal obtained by extracting bitter gourd seed with supercritical carbon dioxide, and sieving with No. 2 sieve. Weighing 100kg, adding 1000L of water, adding 100g of food-grade neutral protease with the enzyme activity of 30 wu/g, adjusting the pH value to 7.0-8.0, heating to 50 ℃, stirring for 2h, boiling for 5min, cooling, passing through a microfiltration membrane with the pore diameter of 0.5 mu m, concentrating until the relative density is 1.2g/mL at 50 ℃, and performing spray drying to obtain 26.4kg of a bitter melon seed extract (Z-1), wherein the total sugar content is 12.8 wt%, the peptide content is 60.1 wt%, the polyphenol content is 6.2 wt%, and the flavone content is 5.5 wt%.
Preparation of example 2
Crushing bitter gourd seed meal obtained by cold pressing bitter gourd seeds and degreasing the bitter gourd seeds, and sieving the crushed bitter gourd seed meal with a No. 1 sieve. Weighing 100kg, adding 2000L of water, adding 100g of food-grade alkaline enzyme with the enzyme activity of 20 wu/g, adjusting the pH value to 8.5-9.5, heating to 50 ℃, stirring for 1h, boiling for 2min, cooling, passing the supernatant through a microfiltration membrane with the pore diameter of 0.1 mu m, concentrating to the relative density of 1.1g/mL at 80 ℃, and freeze-drying to obtain 25.9kg of the bitter melon seed extract (Z-2), wherein the total sugar content is 13.9 wt%, the peptide content is 62.3 wt%, the polyphenol content is 6.8 wt%, and the flavone content is 5.9 wt%.
Comparative examples
The conventional water extraction method comprises the following steps: crushing bitter gourd seed meal obtained by cold pressing bitter gourd seeds and degreasing the bitter gourd seeds, and sieving the crushed bitter gourd seed meal with a No. 1 sieve. Weighing 100kg, adding 2000L water, reflux extracting for 2 times, each time for 3 hr, mixing extractive solutions, concentrating to relative density of 1.2g/mL at 50 deg.C, and freeze drying to obtain 11.2kg semen Momordicae Charantiae extract (Z-3), with total sugar content of 15.5 wt%, protein content of 8.8 wt%, polyphenol content of 5.5 wt%, and flavone content of 4.1 wt%.
Therefore, the conventional water extraction method can only extract a small amount of protein components, the total sugar content is improved to a certain extent, but the improvement is small, the yield is lower than that of the method, and the process advantages of the method are fully embodied.
Examples of biological activity:
1. DPPH free radical scavenging experiment
1.1 preparation of ethanol solution of DPPH: accurately weighing DPPH 4mg, placing in a 100mL brown volumetric flask, adding 50mL ethanol, performing ultrasonic treatment for 30s, fixing the volume to the scale with the ethanol, and shaking up for later use. It should be used in situ;
1.2 preparation of test solution: precisely weighing appropriate amount of semen Momordicae Charantiae extract, placing in 50mL brown volumetric flask, adding 30mL ethanol, performing ultrasonic treatment for 5min, adding ethanol to desired volume to scale, and shaking;
1.3, operation steps: accurately sucking 2mL of a test solution and 2mL of a DPPH solution, and uniformly mixing; accurately sucking 2mL of test solution and 2mL of ethanol, and uniformly mixing; accurately absorbing 2mL of DPPH solution and 2mL of ethanol, uniformly mixing, standing at room temperature for 30min, measuring absorbance at the wavelength of 515nm, and calculating the free radical clearance according to the following calculation formula: IR% ([ 1- (Ai-Aj)/a0] × 100%;
wherein Ai represents the absorbance of the solution after the solution to be detected and DPPH are mixed;
aj represents the absorbance of the solution after the solution to be detected and the solvent are mixed;
a0 represents the absorbance of a solution after mixing DPPH with a solvent.
2. SRSA superoxide anion radical scavenging experiment
2.10.1 moL/L PBS buffer (pH7.4) preparation: weighing 80g of sodium chloride, 2g of potassium chloride, 2.4g of monopotassium phosphate and 23.1g of dipotassium phosphate trihydrate, putting the mixture into a 1000mL beaker, adding 600mL of distilled water, stirring to dissolve the mixture, adjusting the pH to 7.2 by using hydrochloric acid or sodium hydroxide, transferring the mixture into a 1000mL volumetric flask, adding distilled water to dilute the mixture to a scale, and shaking the mixture uniformly for later use;
preparation of 2.2150. mu. moL/L NBT solution: accurately weighing 12.5mg of NBT, placing the NBT in a 100mL brown volumetric flask, adding distilled water, dissolving the NBT by ultrasonic, fixing the volume to a scale by using the distilled water, and shaking up to obtain the NBT-;
2.360 μmoL/L PMS solution preparation: accurately weighing 18.8mg of PMS, placing the PMS in a volumetric flask with the volume of 1000mL, adding distilled water, dissolving the PMS by ultrasonic treatment, fixing the volume to a scale by using the distilled water, and shaking up the PMS to obtain the PMS-based fuel oil;
2.4468 μmoL/L NADH solution preparation: accurately weighing 33.9mg of NADH, placing in a volumetric flask with 100mL, adding distilled water, dissolving by ultrasonic, fixing the volume to the scale by using the distilled water, and shaking up to obtain the NADH-containing liquid;
2.5 preparation of test solution: precisely weighing appropriate amount of semen Momordicae Charantiae extract, placing in 20mL brown volumetric flask, adding 15mL PBS buffer solution, performing ultrasonic treatment for 5min, fixing volume to scale with PBS buffer solution, and shaking;
2.6 preparation of working solution: putting 1mL of 0.1mol/L PBS buffer solution (pH7.4) into a volumetric flask, adding 1mL of 150 mu moL/L NBT solution, adding 2mL of 468 mu moL/L NADH solution, adding 1mL of 60 mu moL/L PMS solution, stirring uniformly, reacting for 5min at 25 ℃, and measuring the absorbance value at the wavelength of 560 nm;
2.7, operation steps: accurately sucking 0.5mL of test solution and 5mL of the working solution, and uniformly mixing; accurately sucking 0.5mL of test solution and 5mL of distilled water, and uniformly mixing; accurately sucking 5mL of the above working solution and 0.5mL of distilled water, uniformly mixing, immediately measuring the absorbance at 560nm, and calculating the radical clearance according to the following formula:
IR％＝[1-(Ai-Aj)/A0]*100％；
wherein Ai represents the absorbance of the solution after the solution to be tested and the SRSA are mixed;
aj represents the absorbance of the solution after the solution to be detected and the solvent are mixed;
a0 represents the absorbance of the solution after mixing the SRSA and solvent.
3、ABTS+Free radical scavenging experiments
3.1 preparation of PBS buffer: weighing 8g of sodium chloride, 0.2g of potassium chloride, 0.24g of potassium dihydrogen phosphate and 3.62g of disodium hydrogen phosphate dodecahydrate, putting the materials into a 1000mL beaker, adding 800mL of distilled water, stirring to dissolve the materials, adjusting the pH value to 7.4 by using hydrochloric acid or sodium hydroxide, transferring the materials into a 1000mL volumetric flask, adding distilled water to dilute the materials to a scale, and shaking the materials uniformly for later use;
3.2ABTS+preparation of a storage solution: ABTS is weighed to precision+Placing about 78mg into a 20mL brown volumetric flask, adding 15mL distilled water, performing ultrasonic treatment for 5min, and diluting to constant volume with distilled waterGraduating and shaking up. Accurately weighing about 76mg of potassium persulfate, placing the potassium persulfate in a 2mL brown volumetric flask, adding 1mL of distilled water, performing ultrasonic dissolution, fixing the volume to the scale with the distilled water, and shaking up. Accurately sucking 352 mu L of potassium persulfate solution, adding the potassium persulfate solution into the ABTS solution, shaking up, and standing overnight;
3.3ABTS+preparing a working solution: accurately sucking 1mL of storage solution, adding about 65mL of PBS buffer solution, and shaking up; 3.4 preparation of test solution: precisely weighing appropriate amount of semen Momordicae Charantiae extract, placing in 20mL brown volumetric flask, adding 15mL PBS buffer solution, performing ultrasonic treatment for 5min, fixing volume to scale with PBS buffer solution, and shaking;
3.5, operation steps: accurately sucking 0.5mL of test solution and 5mL of ABTS working solution, and uniformly mixing; accurately sucking 0.5mL of test solution and 5mL of PBS buffer solution, and uniformly mixing; accurately pipette 5mL of ABTS working solution and 0.5mL of PBS buffer, mix well, immediately measure absorbance at 734nm, and calculate the radical clearance according to the following formula: IR% ([ 1- (Ai-Aj)/a0] × 100%;
wherein Ai represents the absorbance of the solution after the solution to be tested and the ABTS are mixed;
aj represents the absorbance of the solution after the solution to be detected and the solvent are mixed;
a0 represents the absorbance of the solution after mixing ABTS and solvent.
The seed extracts of the bitter gourd of the preparation examples were prepared in different concentrations for DPPH, SRSA and ABTS, respectively+The results of the radical scavenging experiments are shown in table 1.
TABLE 1 radical scavenging test results of balsam pear seed extract
As can be seen from Table 1, the balsam pear seed extracts (Z-1 and Z-2) prepared by the method of the invention have stronger antioxidant activity and are far higher than the balsam pear seed extracts (Z-3) prepared by the traditional water extraction process. Therefore, the balsam pear seed extract prepared by the invention has the functions of preventing cell aging in human body, improving memory, delaying senility, protecting cardiovascular system, preventing senile dementia and the like, and deeper biological activity is under study.
Claims (10)
1. The balsam pear seed extract for scavenging free radicals is characterized in that the total sugar content of the balsam pear seed extract is not less than 10wt%, the peptide content is 55-65 wt%, the polyphenol content is more than 5wt%, and the flavone content is more than 5 wt%.
2. The extract of bitter gourd seeds for scavenging free radicals as claimed in claim 1, wherein the preparation process comprises: mixing the bitter gourd seed meal with water or alcohol, performing enzyme extraction, concentrating, and drying to obtain the bitter gourd seed extract.
3. The balsam pear seed extract for scavenging free radicals as claimed in claim 2, wherein the concentration is carried out until the relative density of the extract is 1.0-1.2 g/mL at 30 ℃, and the extract is dried to obtain the balsam pear seed extract.
4. The extract of bitter gourd seed for scavenging free radicals as claimed in claim 3, wherein the relative density is 1.0-1.1 g/mL or 1.1-1.15 g/mL or 1.15-1.2 g/mL.
5. The extract of Momordica charantia seeds useful for scavenging free radicals as claimed in claim 1, wherein the Momordica charantia seed meal is derived from Momordica charantia seed meal defatted by hot pressing or cold pressing or supercritical fluid extraction.
6. A method for preparing a seed extract of bitter melon for scavenging free radicals as claimed in any one of claims 1 to 5, comprising the following steps: adding water or alcohol into the bitter gourd seed meal, stirring, performing enzymolysis extraction, concentrating and drying to obtain a bitter gourd seed extract, and the steps are as follows:
(1) crushing the bitter gourd seed meal, and sieving the crushed bitter gourd seed meal with a sieve of more than 40 meshes to obtain bitter gourd seed powder;
(2) mixing a certain amount of bitter gourd seed powder with water or alcohol according to a mass ratio of 1: 10-1: 20, adding biological enzyme accounting for 0.1-0.5% of the mass of the bitter gourd seed powder, adjusting the pH value to 2-10, and stirring at a constant temperature of 35-60 ℃ for at least 2 hours;
(3) after extraction is finished, boiling the extracting solution in the step (2) for 5-10 min, cooling to room temperature, and filtering to obtain filtrate for later use;
(4) and (4) concentrating the filtrate obtained in the step (3) to a relative density of 1.0-1.2 g/mL at 30-80 ℃, and drying to obtain the bitter gourd seed extract.
7. The method for preparing the extract of bitter gourd seeds capable of scavenging free radicals as claimed in claim 6, wherein the biological enzymes used for enzymolysis are one or a mixture of neutral protease with enzyme activity not less than 30 wu/g, papain with enzyme activity not less than 40 wu/g, bromelin with enzyme activity not less than 30 wu/g, alkaline protease with enzyme activity not less than 20 wu/g, acidic protease with enzyme activity not less than 20 wu/g, pepsin with enzyme activity not less than 50 wu/g, pancreatin with enzyme activity not less than 3000u/g, ficin with enzyme activity not less than 5 wu/g, and momordica grosvenori protease with enzyme activity not less than 5 wu/g.
8. A composition comprising the extract of seeds of Momordica charantia useful for scavenging free radicals as claimed in any one of claims 1 to 5, wherein the composition is a pharmaceutically or dietetically acceptable adjuvant;
the formulation of the auxiliary agent is selected from plain tablets, film-coated tablets, sugar-coated tablets, enteric-coated tablets, dispersible tablets, capsules, granules, oral solution or oral suspension, and liquid, emulsion, cream, powder or blocks in cosmetic formulations.
9. Use of the extract of bitter gourd seeds for scavenging free radicals as claimed in any one of claims 1 to 5 for the preparation of food, health food, pharmaceutical or cosmetic for preventing or treating symptoms caused by excess free radicals.
10. Use of the composition of claim 8 for the preparation of a food, health food, pharmaceutical or cosmetic product for the prevention or treatment of symptoms caused by excess free radicals.
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