CN112656832A - Trichosanthes seed extract and industrial preparation method and application thereof - Google Patents
Trichosanthes seed extract and industrial preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a snakegourd seed extract and an industrial preparation method and application thereof, wherein the preparation method comprises the following steps: (1) crushing the snakegourd seed meal to obtain snakegourd seed powder; (2) mixing semen Trichosanthis powder with water or alcohol, adding biological enzyme, and extracting to obtain extractive solution; (3) boiling the extractive solution to inactivate biological enzyme, cooling to room temperature, and filtering; (4) concentrating and drying the filtrate to obtain the trichosanthes seed extract. According to the method, the semen trichosanthis is extracted by using the biological enzyme method, the extraction temperature is mild, the yield is high, and the resource utilization rate is improved; in addition, no organic solvent or toxic and harmful compounds are used in the preparation process, the operation is simple and convenient, the safety is high, the yield is high, the cost is low, the environment is friendly, and the method is suitable for large-scale production; can be used for preparing medicine, food, health product or cosmetic for treating or preventing symptoms caused by excessive free radicals.
Description
Technical Field
The invention relates to the field of deep processing of food, in particular to a snakegourd seed extract and an industrial preparation method and application thereof.
Background
The snakegourd fruit is dry mature fruit of Trichosanthes kirilowii Marim (Trichosanthes kirilowii Marim) or Trichosanthes bilateralis (Trichosanthes rosthornii Harms) belonging to Cucurbitaceae, belongs to common traditional Chinese medicinal materials, is loaded in Shen nong Ben Cao Jing, has the effects of clearing heat and removing phlegm, relieving chest stuffiness and eliminating stagnation, moistening dryness and lubricating intestines and the like, and is used for treating diseases such as lung heat cough, turbid phlegm yellow and thick, chest stuffiness and pain, chest stuffiness and fullness, acute mastitis, pulmonary abscess, swelling and pain of intestinal abscess, constipation and the like. The famous prescriptions of Gua Lou Xiebai Bai Jiu Tang and Gua Lou Xiebai ban Tang for treating chest stuffiness and heart pain from Shang Zhong Jing Yang (treatise on exogenous febrile diseases) of Han Dynasty, Zhang Zhong Jing (Cold-induced miscellaneous diseases) take Gua Lou as the main ingredient. Modern researches show that the snakegourd fruit has various pharmacological activities of improving the cardiovascular system, resisting bacteria, tumors and ulcers, eliminating phlegm, enhancing immunity and the like.
Semen Trichosanthis is its dry mature seed, and semen Trichosanthis is heavy in moistening lung and eliminating phlegm, and relieving constipation, and can be used for dry cough with sticky phlegm, and constipation due to intestinal dryness. The Chinese pharmacopoeia records that the snakegourd peel and the snakegourd seed (35:65) aqueous extract are prepared into snakegourd fruit tablets, and the snakegourd peel and snakegourd seed tablets are used for treating coronary heart disease and angina pectoris and have good curative effect. The semen trichosanthis is mainly produced in Shandong, Sichuan and Anhui provinces and contains abundant oil, sterol, triterpene, glycoside and other substances, wherein the content of fatty oil is about 26 percent, and the content of unsaturated fatty acid is about 67 percent, and the content of the unsaturated fatty acid comprises trichosanthes acid, oleic acid, linoleic acid and linoleic acid, so many researches focus on extraction and property analysis of the semen trichosanthis. The byproduct of the oil extraction of the snakegourd fruit seeds contains about 65 percent of protein and inorganic elements such as calcium, iron, copper, zinc and the like, but at present, most of the byproduct is used as waste, valuable components in the waste cannot be fully extracted and utilized, and huge resource waste and environmental pollution are caused.
At present, the method for obtaining the trichosanthes seed extract mainly comprises the methods of water extraction, alcohol extraction after water extraction, alcohol extraction and water extraction, supercritical carbon dioxide extraction and the like. For example, in chinese patent document, "a method for extracting a pharmaceutical ingredient from semen trichosanthis and its application", which is disclosed in publication No. CN108714161A, the extraction steps are: (1) cleaning; (2) freezing; (3) and (3) protein extraction: adding a hydrochloric acid solution with the weight 30-40 times and the mass concentration of 3-5% into the snakegourd fruit seed powder, stirring and extracting for 80-100min at the temperature of 35-40 ℃ and the rotation speed of 400r/min at 300-; (4) extracting triterpenoid saponin.
However, in the existing extraction method, no matter water extraction, alcohol extraction or alcohol-water mixed extraction, or supercritical carbon dioxide extraction, or other organic solvent extraction is adopted, the protein components in the trichosanthes seed medicinal material cannot be completely extracted, and the resource utilization rate is low.
Aiming at the problem, the invention successfully researches a biological enzyme extraction method through a plurality of tests. According to the method, a small amount of biological enzyme is added into the extracting solution to improve the extraction efficiency, and meanwhile, the protein in the snakegourd seed meal can be converted into peptide substances which are well absorbed and utilized by a human body, the extraction temperature is mild, the extraction frequency is only once, so that the resource utilization rate is greatly improved, the production cost is reduced, and the method is green and environment-friendly and is suitable for large-scale production.
Disclosure of Invention
The invention aims to overcome the problem of low resource utilization rate caused by the fact that protein components in a snakegourd fruit seed medicinal material cannot be extracted completely in the existing snakegourd fruit seed extraction method, and provides a snakegourd fruit seed extract and an industrial preparation method and application thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
a semen Trichosanthis extract has total sugar content of 10wt% or more and peptide content of 75wt% or more.
Preferably, the method for measuring the total sugar content is a phenol-sulfuric acid method, and the method for detecting the peptide content is the peptide content measuring methods described in GB/T22492-.
The industrial preparation method of the snakegourd fruit seed extract comprises the following steps:
(1) crushing the snakegourd seed meal to obtain snakegourd seed powder;
(2) mixing semen Trichosanthis powder with water or alcohol, adding biological enzyme, and extracting to obtain extractive solution;
(3) boiling the extractive solution to inactivate biological enzyme, cooling to room temperature, and filtering;
(4) concentrating the filtrate until the relative density is 1.0-1.2 g/mL at 30-80 ℃, and drying to obtain the trichosanthes seed extract.
In the prior art, the method for obtaining the trichosanthes seed extract is a method of water extraction, or alcohol extraction after water extraction, or water extraction after alcohol extraction, or supercritical carbon dioxide extraction and the like, and has the defects that:
1. protein components in the snakegourd seed medicinal material cannot be completely extracted no matter water extraction, alcohol extraction or alcohol-water mixed extraction, or supercritical carbon dioxide extraction, or other organic solvent extraction is carried out.
2. The extraction is carried out by single water extraction, alcohol extraction or mixed extraction of alcohol and water, and high temperature extraction is needed for many times for improving the yield, but the yield is not ideal, and the resource utilization rate is low, so that the production requirement of environmental protection cannot be met.
3. The supercritical carbon dioxide extraction or other organic solvent extraction is adopted, the cost is high, and the use of other organic solvents can not meet the requirements of environmental protection and safety and no toxicity to human bodies.
Compared with the prior art, the invention has the following advantages:
1. the invention uses biological enzyme to extract, the extraction temperature is mild, the extraction frequency is only once, and the yield is higher than that of the prior art, thereby improving the resource utilization rate and reducing the production cost.
2. The preparation process of the invention does not use any organic solvent or toxic and harmful chemical substances, is safe, has no toxic substance residue, is harmless to human body, and is green and environment-friendly.
3. The invention filters the extracting solution through a membrane, reduces the residue of the dregs of a decoction and improves the clarity of the aqueous solution.
Preferably, in the step (4), the relative density of the filtrate after concentration to 30-80 ℃ is 1.0-1.1 g/mL, or 1.1-1.15 g/mL, or 1.15-1.2 g/mL.
Preferably, the snakegourd seed meal in the step (1) is prepared from snakegourd seed meal degreased by hot pressing or cold pressing or a supercritical fluid extraction method, and snakegourd seed meal is crushed and sieved by a 40-80-mesh sieve to obtain snakegourd seed powder.
Preferably, the snakegourd seed powder and water or alcohol are mixed according to the mass ratio of 1: 10-1: 20 in the step (2), 0.1-0.5% of biological enzyme by mass of the snakegourd seed powder is added, the pH is adjusted to 2-10, and the mixture is stirred and extracted at the constant temperature of 35-60 ℃ for at least 2 hours.
Preferably, the biological enzyme in the step (2) is selected from one or more of neutral protease with the enzyme activity of more than or equal to 30 ten thousand u/g, papain with the enzyme activity of more than or equal to 40 ten thousand u/g, bromelain with the enzyme activity of more than or equal to 30 ten thousand u/g, alkaline protease with the enzyme activity of more than or equal to 20 ten thousand u/g, acid protease, pepsin with the enzyme activity of more than or equal to 50 ten thousand u/g, pancreatin with the enzyme activity of more than or equal to 3000u/g, ficin with the enzyme activity of more than or equal to 5 ten thousand u/g and momordica grosvenori protease with the enzyme activity of more than or equal to.
Preferably, the boiling time in the step (3) is 5-10 min.
The invention also provides a composition which comprises the snakegourd seed extract.
The composition can be used for preparing medicines, foods, health products or cosmetics for treating or preventing symptoms caused by excessive free radicals. The dosage form can be plain tablet, film coated tablet, sugar coated tablet, intestine coated tablet, dispersible tablet, capsule, granule, oral solution or oral suspension, and cosmetic dosage forms such as liquid, emulsion, cream, powder, and block.
The snakegourd seed extract prepared by the invention is rich in saccharides and peptides, has good antioxidant activity, can be used in food, medicine, health care products or cosmetics to treat or prevent symptoms caused by excessive free radicals, and has the effects of preventing cell aging in a human body, improving memory, delaying aging, protecting cardiovascular system, preventing senile dementia and the like.
Therefore, the invention has the following beneficial effects:
(1) the method has the advantages that the biological enzyme is used for extracting the snakegourd fruit seeds, the extraction temperature is mild, the extraction times are only one time, and the yield is higher than that of the prior art, so that the resource utilization rate is improved, and the production cost is reduced;
(2) no organic solvent or toxic and harmful chemical substances are used in the preparation process, and the preparation method is safe, free of toxic substance residues, harmless to human bodies and environment-friendly;
(3) the extracting solution is filtered by a membrane, so that the residue of the dregs of a decoction is reduced, and the clarity of the aqueous solution is improved.
Detailed Description
The invention is further described with reference to specific embodiments.
All materials and solvents used in the examples of the present invention were purchased from Sigma Biochemical and Organic Compounds for Research and Diagnostic Clinical Reagents, Inc.;
the method for measuring the total sugar content adopts a phenol-sulfuric acid method;
the detection method of the peptide content refers to the peptide content determination method described in GB/T22492-;
the method for measuring the polyphenol content is a forskolin phenol measuring method.
Example 1:
an industrial preparation method of a snakegourd fruit seed extract comprises the following steps:
(1) pulverizing semen Trichosanthis seed meal obtained by supercritical carbon dioxide extraction, and sieving with No. 2 sieve to obtain semen Trichosanthis powder;
(2) weighing 100kg of snakegourd seed powder, adding 1000L of water, adding 100g of food-grade neutral protease with the enzyme activity of 30 ten thousand u/g, adjusting the pH to 7.5, heating to 50 ℃, and stirring and extracting for 2 hours to obtain an extracting solution;
(3) boiling the extractive solution for 5min to inactivate biological enzyme, cooling to room temperature, and passing the supernatant through microfiltration membrane with pore diameter of 0.5 μm;
(4) the filtrate was concentrated to a relative density of 1.2g/mL at 50 ℃ and spray dried to yield 67.0kg of trichosanthes seed extract.
The total sugar content of the trichosanthes seed extract prepared in the example is 14.4 wt%, and the peptide content is 76.2 wt%.
Example 2:
an industrial preparation method of a snakegourd fruit seed extract comprises the following steps:
(1) crushing the snakegourd seed meal obtained by cold pressing and degreasing snakegourd seeds, and sieving the crushed snakegourd seed meal with a No. 1 sieve to obtain snakegourd seed powder;
(2) weighing 100kg of snakegourd seed powder, adding 2000L of water, adding 100g of food-grade alkaline protease with the enzyme activity of 20 ten thousand u/g, adjusting the pH to 9.0, heating to 60 ℃, and stirring and extracting for 2 hours to obtain an extracting solution;
(3) boiling the extractive solution for 8min to inactivate biological enzyme, cooling to room temperature, and passing the supernatant through microfiltration membrane with pore diameter of 0.1 μm;
(4) the filtrate was concentrated to a relative density of 1.1g/mL at 80 ℃ and freeze-dried to give 65.3kg of trichosanthes seed extract.
The total sugar content of the trichosanthes seed extract prepared in the example is 13.9 wt%, and the peptide content is 77.5 wt%.
Comparative example 1:
an industrial preparation method of a snakegourd fruit seed extract comprises the following steps:
(1) crushing the snakegourd seed meal obtained by cold pressing and degreasing snakegourd seeds, and sieving the crushed snakegourd seed meal with a No. 1 sieve to obtain snakegourd seed powder;
(2) weighing 100kg of semen Trichosanthis powder, adding 2000L of water, reflux-extracting for 2 times, each for 3 hr, and mixing extractive solutions;
(3) concentrating the extractive solution to relative density of 1.2g/mL at 50 deg.C, and freeze drying to obtain 20.8kg semen Trichosanthis extract.
The total sugar content of the trichosanthes seed extract prepared in the example is 24.0 wt%, and the peptide content is 28.7 wt%.
It can be seen from the above examples and comparative examples that the comparative example 1 can only extract a small amount of protein components by using the conventional water extraction method, the total sugar content is improved to a certain extent, but the improvement is small, the yield is lower than that of the trichosanthes seed extract prepared by using the method in the examples, and the process advantages of the invention are fully embodied.
Respectively adopting DPPH radical scavenging method, SRSA superoxide anion radical scavenging method and ABTS+The antioxidant activity of the trichosanthes seed extract prepared in the examples was tested by the radical scavenging method.
The test method comprises the following steps:
1. DPPH free radical scavenging experiment
1.1 preparation of ethanol solution of DPPH: accurately weighing DPPH 4mg, placing in a 100mL brown volumetric flask, adding 50mL ethanol, performing ultrasonic treatment for 30s, fixing the volume to the scale with the ethanol, and shaking up for later use; it should be used in situ;
1.2 preparation of test solution: precisely weighing appropriate amount of semen Trichosanthis extract, placing in 50mL brown volumetric flask, adding 30mL ethanol, performing ultrasonic treatment for 5min, adding ethanol to desired volume to scale, and shaking;
1.3, operation steps: accurately sucking 2mL of a test solution and 2mL of a DPPH solution, and uniformly mixing; accurately sucking 2mL of test solution and 2mL of ethanol, and uniformly mixing; accurately absorbing 2mL of DPPH solution and 2mL of ethanol, uniformly mixing, standing at room temperature for 30min, measuring absorbance at the wavelength of 515nm, and calculating the free radical clearance according to the following calculation formula: IR% ([ 1- (Ai-Aj)/a0] × 100%;
wherein Ai represents the absorbance of the solution after the solution to be detected and DPPH are mixed;
aj represents the absorbance of the solution after the solution to be detected and the solvent are mixed;
a0 represents the absorbance of a solution after mixing DPPH with a solvent.
2. SRSA superoxide anion radical scavenging experiment
2.10.1 moL/L PBS buffer (pH7.4) preparation: weighing 80g of sodium chloride, 2g of potassium chloride, 2.4g of monopotassium phosphate and 23.1g of dipotassium phosphate trihydrate, putting the mixture into a 1000mL beaker, adding 600mL of distilled water, stirring to dissolve the mixture, adjusting the pH to 7.2 by using hydrochloric acid or sodium hydroxide, transferring the mixture into a 1000mL volumetric flask, adding distilled water to dilute the mixture to a scale, and shaking the mixture uniformly for later use;
preparation of 2.2150. mu. moL/L NBT solution: accurately weighing 12.5mg of NBT, placing the NBT in a 100mL brown volumetric flask, adding distilled water, dissolving the NBT by ultrasonic, fixing the volume to a scale by using the distilled water, and shaking up to obtain the NBT-;
2.360 μmoL/L PMS solution preparation: accurately weighing 18.8mg of PMS, placing the PMS in a volumetric flask with the volume of 1000mL, adding distilled water, dissolving the PMS by ultrasonic treatment, fixing the volume to a scale by using the distilled water, and shaking up the PMS to obtain the PMS-based fuel oil;
2.4468 μmoL/L NADH solution preparation: accurately weighing 33.9mg of NADH, placing in a volumetric flask with 100mL, adding distilled water, dissolving by ultrasonic, fixing the volume to the scale by using the distilled water, and shaking up to obtain the NADH-containing liquid;
2.5 preparation of test solution: precisely weighing a proper amount of snakegourd seed extract, placing the snakegourd seed extract in a 20mL brown volumetric flask, adding 15mL of PBS buffer solution, carrying out ultrasonic treatment for 5min, fixing the volume to a scale with the PBS buffer solution, and shaking up to obtain the snakegourd seed extract;
2.6 preparation of working solution: taking 1mL of 0.1moL/L PBS buffer solution (pH7.4) to a volumetric flask, adding 1mL of 150 mu moL/L NBT solution, adding 2mL of 468 mu moL/L NADH solution, adding 1mL of 60 mu moL/L PMS solution, stirring uniformly, reacting at 25 ℃ for 5min, and measuring the absorbance value at the wavelength of 560 nm;
2.7, operation steps: accurately sucking 0.5mL of test solution and 5mL of the working solution, and uniformly mixing; accurately sucking 0.5mL of test solution and 5mL of distilled water, and uniformly mixing; accurately sucking 5mL of the above working solution and 0.5mL of distilled water, uniformly mixing, immediately measuring the absorbance at 560nm, and calculating the radical clearance according to the following formula:
IR％＝[1-(Ai-Aj)/A0]*100％；
wherein Ai represents the absorbance of the solution after the solution to be tested and the SRSA are mixed;
aj represents the absorbance of the solution after the solution to be detected and the solvent are mixed;
a0 represents the absorbance of the solution after mixing the SRSA and solvent.
3、ABTS+Free radical scavenging experiments
3.1 preparation of PBS buffer: weighing 8g of sodium chloride, 0.2g of potassium chloride, 0.24g of potassium dihydrogen phosphate and 3.62g of disodium hydrogen phosphate dodecahydrate, putting the materials into a 1000mL beaker, adding 800mL of distilled water, stirring to dissolve the materials, adjusting the pH value to 7.4 by using hydrochloric acid or sodium hydroxide, transferring the materials into a 1000mL volumetric flask, adding distilled water to dilute the materials to a scale, and shaking the materials uniformly for later use;
3.2ABTS+preparation of a storage solution: ABTS is weighed to precision+Placing the mixture into a 20mL brown volumetric flask, adding 15mL distilled water, carrying out ultrasonic treatment for 5min, fixing the volume to the scale with the distilled water, and shaking up. Accurately weighing about 76mg of potassium persulfate, placing the potassium persulfate in a 2mL brown volumetric flask, adding 1mL of distilled water, performing ultrasonic dissolution, fixing the volume to the scale with the distilled water, and shaking up. Add 352. mu.L of Potassium persulfate solution to ABTS with precision suction+Shaking up in the solution, and standing overnight;
3.3 ABTS+preparing a working solution: accurately sucking 1mL of storage solution, adding about 65mL of PBS buffer solution, and shaking up;
3.4 preparation of test solution: precisely weighing a proper amount of snakegourd seed extract, placing the snakegourd seed extract in a 20mL brown volumetric flask, adding 15mL of PBS buffer solution, carrying out ultrasonic treatment for 5min, fixing the volume to a scale with the PBS buffer solution, and shaking up to obtain the snakegourd seed extract;
3.5, operation steps: accurately sucking 0.5mL of test solution and 5mL of ABTS+Uniformly mixing the working solution; accurately sucking 0.5mL of test solution and 5mL of PBS buffer solution, and uniformly mixing; accurately absorb 5mL of ABTS+The working solution was mixed well with 0.5mL of PBS buffer, the absorbance was immediately measured at 734nm, and the radical clearance was calculated according to the following formula: IR% [1- (Ai-Aj)/A0]*100％；
Wherein Ai represents the absorbance of the solution after the solution to be tested and the ABTS are mixed;
aj represents the absorbance of the solution after the solution to be detected and the solvent are mixed;
a0 represents the absorbance of the solution after mixing ABTS and solvent.
The extracts of Trichosanthes kirilowii prepared in examples and comparative examples were used to prepare solutions of Trichosanthes kirilowii extracts at concentrations of 0.5 and 1mg/mL, respectively, for DPPH, SRSA, and ABTS+Radical scavenging experiments, the results of which are shown in table 1.
Table 1: the results of experiments on scavenging free radicals of the trichosanthes seed extract.
As can be seen from table 1, the trichosanthes seed extract prepared by the method of the present invention in the examples has a stronger antioxidant activity, which is much higher than that of the trichosanthes seed extract prepared by the conventional water extraction process in comparative example 1. Therefore, the snakegourd seed extract prepared by the invention can be used for preparing medicines, foods, health-care products or cosmetics for treating or preventing symptoms caused by excessive free radicals, and has the effects of preventing cell aging in a human body, improving memory, delaying aging, protecting cardiovascular system, preventing senile dementia and the like.
Claims (10)
1. A semen trichosanthis extract is characterized in that the total sugar content is not less than 10wt%, and the peptide content is more than 75 wt%.
2. The trichosanthes seed extract as claimed in claim 1, which is characterized in that the determination method of the total sugar content is phenol-sulfuric acid method, and the determination method of the peptide content is the peptide content determination method described in GB/T22492-.
3. An industrial preparation method of the trichosanthes seed extract according to claim 1 or 2, which is characterized by comprising the following steps:
(1) crushing the snakegourd seed meal to obtain snakegourd seed powder;
(2) mixing semen Trichosanthis powder with water or alcohol, adding biological enzyme, and extracting to obtain extractive solution;
(3) boiling the extractive solution to inactivate biological enzyme, cooling to room temperature, and filtering;
(4) concentrating the filtrate until the relative density is 1.0-1.2 g/mL at 30-80 ℃, and drying to obtain the trichosanthes seed extract.
4. The industrial preparation method of the snakegourd seed extract as claimed in claim 3, wherein the relative density of the filtrate at 30-80 ℃ is 1.0-1.1 g/mL, 1.1-1.15 g/mL or 1.15-1.2 g/mL in step (4).
5. The industrial preparation method of the snakegourd seed extract as claimed in claim 3, wherein the snakegourd seed meal in the step (1) is prepared from snakegourd seed meal degreased by hot pressing or cold pressing or supercritical fluid extraction, and the snakegourd seed meal is crushed and sieved by a sieve of 10-50 meshes to obtain snakegourd seed powder.
6. The industrial preparation method of the snakegourd seed extract as claimed in claim 3, wherein in the step (2), snakegourd seed powder and water or alcohol are mixed according to the mass ratio of 1: 10-1: 20, biological enzyme accounting for 0.1-0.5% of the mass of the snakegourd seed powder is added, the pH is adjusted to 2-10, and the mixture is stirred and extracted at the constant temperature of 35-60 ℃ for at least 2 hours.
7. The industrial preparation method of a snakegourd seed extract as claimed in claim 3 or 6, wherein the biological enzyme in step (2) is selected from one or more of neutral protease with an enzyme activity of more than or equal to 30 ten thousand u/g, papain with an enzyme activity of more than or equal to 40 ten thousand u/g, bromelain with an enzyme activity of more than or equal to 30 ten thousand u/g, alkaline protease with an enzyme activity of more than or equal to 20 ten thousand u/g, acid protease, pepsin with an enzyme activity of more than or equal to 50 ten thousand u/g, pancreatin with an enzyme activity of more than or equal to 3000u/g, ficin with an enzyme activity of more than or equal to 5 ten thousand u/g, and momordica grosvenori proteinase with an enzyme activity of more than or equal to 5 ten.
8. The industrial preparation method of the snakegourd fruit seed extract as claimed in claim 3, wherein the step (3) is boiling for 5-10 min.
9. A composition comprising the trichosanthes seed extract of claim 1 or 2.
10. Use of a composition according to claim 9 for the preparation of a medicament, food, health product or cosmetic for the treatment or prevention of symptoms caused by excess free radicals.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116671638A (en) * | 2023-06-15 | 2023-09-01 | 江西维莱营健高科有限公司 | Casein calcium tablet and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613649A (en) * | 2012-03-29 | 2012-08-01 | 何家庆 | Semi-skimmed trichosanthes kirilowii maxim protein beverage and preparation method thereof |
| CN104045684A (en) * | 2014-06-27 | 2014-09-17 | 桐城市牯牛背农业开发有限公司 | Production process for extracting snakegourd fruit seed proteins |
| CN111249338A (en) * | 2018-12-03 | 2020-06-09 | 杏辉天力（杭州）药业有限公司 | Cistanche deserticola extract and industrial preparation method and application thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613649A (en) * | 2012-03-29 | 2012-08-01 | 何家庆 | Semi-skimmed trichosanthes kirilowii maxim protein beverage and preparation method thereof |
| CN104045684A (en) * | 2014-06-27 | 2014-09-17 | 桐城市牯牛背农业开发有限公司 | Production process for extracting snakegourd fruit seed proteins |
| CN111249338A (en) * | 2018-12-03 | 2020-06-09 | 杏辉天力（杭州）药业有限公司 | Cistanche deserticola extract and industrial preparation method and application thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116671638A (en) * | 2023-06-15 | 2023-09-01 | 江西维莱营健高科有限公司 | Casein calcium tablet and preparation method thereof |
| CN116671638B (en) * | 2023-06-15 | 2024-03-22 | 江西维莱营健高科有限公司 | Casein calcium tablet and preparation method thereof |
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