CN114904294A - Preparation method of high-yield tea flavone - Google Patents
Preparation method of high-yield tea flavone Download PDFInfo
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- CN114904294A CN114904294A CN202210751110.6A CN202210751110A CN114904294A CN 114904294 A CN114904294 A CN 114904294A CN 202210751110 A CN202210751110 A CN 202210751110A CN 114904294 A CN114904294 A CN 114904294A
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- flavone
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- 229930003944 flavone Natural products 0.000 title claims abstract description 69
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- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 claims description 7
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- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 claims description 7
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- 238000004108 freeze drying Methods 0.000 claims description 6
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- 230000009286 beneficial effect Effects 0.000 abstract description 3
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- 239000003094 microcapsule Substances 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 9
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 6
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 description 2
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- IYRMWMYZSQPJKC-UHFFFAOYSA-N kaempferol Chemical compound C1=CC(O)=CC=C1C1=C(O)C(=O)C2=C(O)C=C(O)C=C2O1 IYRMWMYZSQPJKC-UHFFFAOYSA-N 0.000 description 2
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 2
- 230000031700 light absorption Effects 0.000 description 2
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 2
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- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- IKMDFBPHZNJCSN-UHFFFAOYSA-N Myricetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC(O)=C(O)C(O)=C1 IKMDFBPHZNJCSN-UHFFFAOYSA-N 0.000 description 1
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- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 description 1
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- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
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- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
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- UXOUKMQIEVGVLY-UHFFFAOYSA-N morin Natural products OC1=CC(O)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UXOUKMQIEVGVLY-UHFFFAOYSA-N 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- PCOBUQBNVYZTBU-UHFFFAOYSA-N myricetin Natural products OC1=C(O)C(O)=CC(C=2OC3=CC(O)=C(O)C(O)=C3C(=O)C=2)=C1 PCOBUQBNVYZTBU-UHFFFAOYSA-N 0.000 description 1
- 235000007743 myricetin Nutrition 0.000 description 1
- 229940116852 myricetin Drugs 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- XKLJHFLUAHKGGU-UHFFFAOYSA-N nitrous amide Chemical compound ON=N XKLJHFLUAHKGGU-UHFFFAOYSA-N 0.000 description 1
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
- B01D11/028—Flow sheets
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
- B01D11/0261—Solvent extraction of solids comprising vibrating mechanisms, e.g. mechanical, acoustical
- B01D11/0265—Applying ultrasound
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
- C07D311/26—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
- C07D311/28—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only
- C07D311/30—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only not hydrogenated in the hetero ring, e.g. flavones
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
- C07D311/26—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
- C07D311/40—Separation, e.g. from natural material; Purification
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Acoustics & Sound (AREA)
- Life Sciences & Earth Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mechanical Engineering (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a preparation method of high-yield tea flavone, belonging to the technical field of tea flavone extraction. The invention is to dry and crush fresh white tea to obtain tea powder; then tea powder is firstly subjected to enzymolysis, and then the enzymolysis liquid is subjected to microcapsule-assisted ultrasonic extraction. In the invention, citric acid-sodium citrate buffer solution is used as solution during enzymolysis treatment, and cellulase is added for enzymolysis operation, which is beneficial to improving the extraction amount of flavone. According to the invention, ethanol is used as an extracting agent during ultrasonic extraction, and beta-cyclodextrin is added to carry out embedding protection on tea flavone, so that the extraction amount of the tea flavone is obviously improved; meanwhile, the obtained tea flavone has higher antioxidant activity because of the lower temperature of enzymolysis-ultrasonic extraction.
Description
Technical Field
The invention belongs to the technical field of tea flavone extraction, and particularly relates to a preparation method of high-yield tea flavone.
Background
The white tea is one of the rarity in Chinese tea, belonging to micro-fermented tea. The white tea has various medicinal values and efficacies and is widely collected. The white tea is rich in alkaloid, vitamins, tea polysaccharide, tea flavone, tea polyphenol, amino acid, tea pigment and other components, and has the health functions of resisting cancer, delaying senility, reducing blood fat, reducing blood sugar, reducing blood pressure and the like. Flavone is a main active ingredient in white tea, and white tea contains more flavonoids such as rutin, myricetin, quercetin and kaempferol.
The white tea flavone has high free radical scavenging effect and strong anti-lipid peroxidation activity. Meanwhile, the flavonoids in the white tea can also inhibit excessive platelet aggregation, so that cardiovascular diseases caused by the platelets can be inhibited, and the risk of coronary heart disease is reduced. In addition, the anticancer activity of flavone is receiving more and more attention. The white tea flavone has effect in inhibiting mutation caused by ultraviolet rays, various carcinogenic chemicals or nitrogen-containing compounds such as nitrosamine, etc., thereby inhibiting growth of tumor cells.
At present, the method for extracting the white tea flavone from the white tea mainly comprises an extraction method and a hot reflux method, but the extraction time of the former is longer and the efficiency is not high, while the temperature required by the latter is higher, which can cause the activity of the effective component to be inhibited. In recent years, the ultrasonic-assisted extraction technology has the characteristics of convenient operation, less time consumption, high extraction efficiency and the like, and is widely applied to extraction of various food chemical components. However, the existing ultrasonic-assisted extraction technology still has the problem of low extraction rate of tea flavone.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a preparation method of high-yield tea flavone, which combines a seed enzyme method and ultrasonic wave assistance to reduce the extraction temperature and extraction time, and obviously improves the extraction rate of the tea flavone through embedding of beta-cyclodextrin.
The preparation method of the high-yield tea flavone comprises the following steps:
(1) drying and crushing fresh white tea to obtain tea powder;
(2) adding the tea powder into a citric acid-sodium citrate buffer solution according to the material-liquid ratio of 1: 10-15, uniformly mixing, and adjusting the pH value to 5-6 to obtain a mixed slurry;
(3) adding cellulase into the mixed slurry, wherein the addition amount of the cellulase is 100-;
(4) adding ethanol and beta-cyclodextrin into the enzymolysis liquid to obtain a material to be extracted, and performing ultrasonic extraction on the material to be extracted, wherein the ultrasonic power is 210W, the extraction temperature is 40-60 ℃, and the extraction time is 30-60min to obtain a flavone extract liquid;
(5) and (3) concentrating the flavone extracting solution under reduced pressure, and freeze-drying to obtain a tea flavone solid product.
Preferably, the granularity of the tea powder in the step (1) is controlled to be 50-100 meshes, the enzymolysis temperature in the step (3) is 45-60 ℃, and the enzymolysis time is 60-100 min.
Preferably, the mass concentration of ethanol in the material to be extracted in the step (4) is 50-60%, and the addition amount of the beta-cyclodextrin accounts for 1-2% of the mass of the enzymolysis liquid.
Preferably, the method also comprises the step of preserving the tea flavone solid product in a low-temperature sealing way.
Further preferably, the temperature for storing the low-temperature seal is 2-8 ℃.
Compared with the prior art, the invention has the following beneficial effects:
the invention is to dry and crush fresh white tea to obtain tea powder; then tea powder is firstly subjected to enzymolysis, and then the enzymolysis liquid is subjected to ultrasonic extraction and embedded by combining beta-cyclodextrin. According to the invention, during enzymolysis treatment, citric acid-sodium citrate buffer solution is used as a solution, and cellulase is added for enzymolysis, so that the release of flavone in tea tissues in the extraction process is facilitated after enzymolysis, and the extraction efficiency is improved. The invention adopts ethanol as an extracting agent during ultrasonic extraction, and simultaneously adds beta-cyclodextrin, thereby reducing partial decomposition of tea flavone caused by heating, and being beneficial to maintaining higher activity of the tea flavone while improving the extraction amount of the tea flavone. The extraction method of the invention improves the extraction rate of the tea flavone by 23.5 percent compared with the comparative example, and improves the in vitro antioxidant activity of the tea flavone to a certain extent.
Detailed Description
The present invention will be described in detail with reference to the following embodiments in order to make the objects, features and advantages thereof more comprehensible. Several embodiments of the invention are given below. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Example 1
A preparation method of high-yield tea flavone comprises the following steps:
(1) drying and crushing fresh white tea to obtain tea powder with the granularity of 100 meshes;
(2) adding the tea powder into a citric acid-sodium citrate buffer solution according to the material-liquid ratio of 1:12, uniformly mixing, and adjusting the pH value to 5 to obtain a mixed slurry;
(3) adding cellulase into the mixed slurry, wherein the addition amount of the cellulase is 150U/g, and carrying out enzymolysis treatment to obtain an enzymolysis liquid;
(4) adding ethanol and beta-cyclodextrin into the enzymolysis liquid to obtain a material to be extracted, wherein the mass concentration of the ethanol in the material to be extracted is 55%, the adding amount of the beta-cyclodextrin accounts for 1.5% of the mass of the enzymolysis liquid, and performing ultrasonic extraction on the material to be extracted, wherein the ultrasonic power is 210W, the extraction temperature is 45 ℃, and the extraction time is 50min, so as to obtain a flavone extract liquid;
(5) concentrating the flavone extract under reduced pressure, freeze drying to obtain tea flavone solid product with flavone extraction amount of 292mg rutin equivalent/g white tea, and sealing and storing the tea flavone solid product at 5 deg.C.
Example 2
A preparation method of high-yield tea flavone comprises the following steps:
(1) drying and crushing fresh white tea to obtain tea powder with the particle size of 75 meshes;
(2) adding the tea powder into a citric acid-sodium citrate buffer solution according to the material-liquid ratio of 1:10, uniformly mixing, and adjusting the pH value to 6 to obtain a mixed slurry;
(3) adding cellulase into the mixed slurry, wherein the addition amount of the cellulase is 100U/g, and carrying out enzymolysis treatment to obtain an enzymolysis liquid;
(4) adding ethanol and beta-cyclodextrin into the enzymolysis liquid to obtain a material to be extracted, wherein the mass concentration of the ethanol in the material to be extracted is 50%, the addition amount of the beta-cyclodextrin accounts for 2% of the mass of the enzymolysis liquid, and performing ultrasonic extraction on the material to be extracted, wherein the ultrasonic power is 210W, the extraction temperature is 45 ℃, and the extraction time is 50min, so as to obtain a flavone extract liquid;
(5) concentrating the flavone extract under reduced pressure, freeze drying to obtain tea flavone solid product with flavone extraction amount of 276mg rutin equivalent/g white tea, and sealing and storing the tea flavone solid product at 5 deg.C.
Example 3
A preparation method of high-yield tea flavone comprises the following steps:
(1) drying and crushing fresh white tea to obtain tea powder with the granularity of 100 meshes;
(2) adding the tea powder into a citric acid-sodium citrate buffer solution according to the material-liquid ratio of 1:15, uniformly mixing, and adjusting the pH value to 6 to obtain a mixed slurry;
(3) adding cellulase into the mixed slurry, wherein the addition amount of the cellulase is 150U/g, and carrying out enzymolysis treatment to obtain an enzymolysis liquid;
(4) adding ethanol and beta-cyclodextrin into the enzymolysis liquid to obtain a material to be extracted, wherein the mass concentration of the ethanol in the material to be extracted is 60%, the adding amount of the beta-cyclodextrin accounts for 2% of the mass of the enzymolysis liquid, and performing ultrasonic extraction on the material to be extracted, wherein the ultrasonic power is 210W, the extraction temperature is 50 ℃, and the extraction time is 50min, so as to obtain a flavone extract liquid;
(5) concentrating the flavone extract under reduced pressure, freeze drying to obtain tea flavone solid product with flavone extraction amount of 315mg rutin equivalent/g white tea, and finally sealing and storing the tea flavone solid product at 5 ℃.
Comparative example 1
A preparation method of high-yield tea flavone comprises the following steps:
(1) drying the white tea sample in a 50 ℃ oven to constant weight, crushing by using a crusher, sieving by using a 80-mesh sieve, and storing in a dryer.
(2) Methanol is selected as a solvent, the temperature is 50 ℃, the ultrasonic power is 210W, and the ratio of material to liquid is 1: 20, extracting for 50min to obtain flavone extract;
(3) concentrating the flavone extract under reduced pressure, freeze drying to obtain tea flavone solid product with flavone extraction amount of 255mg rutin equivalent/g white tea, and finally sealing and storing the tea flavone solid product at 5 ℃.
The tea flavonoids prepared in example 1 and comparative example 1 were tested for in vitro activity as follows:
1) DPPH radical scavenging ability
Respectively weighing 1mg of tea flavone, adding 1mL of ultrapure water to prepare 1mg/mL of sample solution, placing the sample solution in a 2mL centrifuge tube, and diluting the sample solution into 250 mu g/mL of sample solution by using the ultrapure water; weighing 2.37mg of DPPH free radical, adding 10mL of ethanol to prepare DPPH free radical solution (storing in dark place), sequentially adding 100 microliter of sample solution, 100 microliter of ethanol and 100 microliter of DPPH free radical solution into each hole of a 96-hole plate at room temperature, fully and uniformly mixing, keeping dark for 30min, and measuring light absorption temperature (A) at 515 nm; sample solutions were replaced with 100. mu.L of ultrapure water as a blank control (A blank), each set was triplicated, and Glutathione Standards (GSH) as a positive control.
DPPH free radical clearance (%) < A empty-A/A empty x 100%
When the concentration of the tea flavonoids of the example 1 and the comparative example 1 is 250 mu g/mL, the DPPH free radical scavenging capacity of the tea flavonoids of the example 1 reaches 75.6 percent, and the DPPH free radical scavenging capacity of the tea flavonoids of the comparative example 1 reaches 68.5 percent;
2) OH radical scavenging ability
The specific detection method comprises the following steps: 1mL of FeSO was added to a 10mL test tube in sequence 4 (6mmol/L) solution, 1mL of tea flavone solution to be tested (250. mu.g/mL), 1mL of H 2 O 2 Starting the reaction by using the (6mmol/L) solution, standing for 10min at room temperature, adding 1mL of salicylic acid (6mmol/L) solution as a capture agent, standing for 30min at room temperature, taking 200 mu L of supernatant liquid, placing the supernatant liquid in a 96-well plate, and measuring the absorbance at the wavelength of 510nm by using a microplate reader. The positive control group uses reduced glutathione to replace the sample, emptyUltrapure water for white group to replace H 2 O 2 The control group replaced the samples with water, each sample was done in 3 replicates and the results averaged.
When the concentration of the tea flavone in example 1 and the concentration of the tea flavone in comparative example 1 are both 250 mu g/mL, the OH free radical scavenging capacity of the tea flavone in example 1 of the invention can reach 48.6 percent, and the OH free radical scavenging capacity of the tea flavone in comparative example 1 is 41.5 percent.
OH free radical clearance (%) < 1- (Aye-Aow)/Aow × 100%
3) The specific detection method of the total reducing force light absorption value is as follows:
0.5mL of a tea flavonoid solution to be tested (250. mu.g/mL), 0.5mL of a phosphate buffer solution (0.2mol/L, pH 6.6) and 2.5mL of 1% potassium ferricyanide were sequentially added to a 10mL centrifuge tube, and then subjected to a 50 ℃ water bath for 20min, followed by 0.5mL of 10% TCA and centrifugation (3000rpm,10 min). Taking 100 mu L of supernatant, 70 mu L of ultrapure water and 70 mu L of 0.1% FeCl 3 The reaction was carried out in a 96-well plate at room temperature for 10min, and the absorbance was measured at a wavelength of 700nm with a microplate reader. And the positive control group uses reduced glutathione to replace the sample, the blank group uses ultrapure water to replace the sample, and the total reducing force is measured according to a formula. Each sample was done 3 times in parallel and the results averaged.
Total reducing power (A sample-A empty) x 100
When the concentration of the tea flavone in example 1 and the concentration of the tea flavone in comparative example 1 are both 250 mug/mL, the total reducing force absorbance value of the tea flavone in the example 1 of the invention is 0.63, and the total reducing force absorbance value of the tea flavone in the comparative example 1 is 0.58.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (6)
1. A preparation method of high-yield tea flavone is characterized by comprising the following steps:
(1) drying and crushing fresh white tea to obtain tea powder;
(2) adding the tea powder into a citric acid-sodium citrate buffer solution according to the material-liquid ratio of 1: 10-15, uniformly mixing, and adjusting the pH value to 5-6 to obtain a mixed slurry;
(3) adding cellulase into the mixed slurry, wherein the addition amount of the cellulase is 100-200U/g, and carrying out enzymolysis treatment to obtain an enzymolysis solution;
(4) adding ethanol and beta-cyclodextrin into the enzymolysis liquid to obtain a material to be extracted, and performing ultrasonic extraction on the material to be extracted, wherein the ultrasonic power is 210W, the extraction temperature is 40-60 ℃, and the extraction time is 30-60min to obtain a flavone extract liquid; rutin is used as a standard substance, and the content of flavone in the rutin is detected.
(5) And (3) concentrating the flavone extracting solution under reduced pressure, and freeze-drying to obtain a tea flavone solid product.
2. The method for preparing high-yield tea flavonoids according to claim 1, wherein the particle size of the tea powder in the step (1) is controlled to be 50-100 meshes.
3. The method for preparing high-yield tea flavonoids according to claim 1, wherein the enzymolysis temperature in the step (3) is 45-60 ℃ and the enzymolysis time is 60-100 min.
4. The method for preparing high-yield tea flavonoids according to claim 1, wherein the mass concentration of ethanol in the material to be extracted in the step (4) is 50-60%, and the addition amount of the beta-cyclodextrin accounts for 1-2% of the mass of the enzymolysis liquid.
5. The method for preparing high-yield tea flavonoids according to claim 1 further comprises the step of storing the tea flavonoids solid product in a low-temperature sealed state.
6. The method for preparing high-yield tea flavonoids according to claim 5, wherein the low-temperature sealing preservation temperature is 2-8 ℃.
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