CN109744540A - The extracting method of melanoidin in a kind of black garlic - Google Patents
The extracting method of melanoidin in a kind of black garlic Download PDFInfo
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- CN109744540A CN109744540A CN201910196839.XA CN201910196839A CN109744540A CN 109744540 A CN109744540 A CN 109744540A CN 201910196839 A CN201910196839 A CN 201910196839A CN 109744540 A CN109744540 A CN 109744540A
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Abstract
The present invention provides a kind of extracting methods of melanoidin in black garlic, belong to the preparation and processing technique field of biological product.Extracting method of the present invention includes the following steps: that (1) mixes the black garlic of peeling with water, grinding, obtains black garlic slurry；(2) black garlic slurry is placed under the conditions of high static pressure and carries out activation processing；(3) by activation treated the black garlic slurry centrifugation, supernatant is obtained；(4) ungrease treatment is carried out to the supernatant, obtains degreasing black garlic juice；(5) dialysis treatment is carried out to the degreasing black garlic juice, obtains retaining liquid；(6) the retention liquid is dried, obtains melanoidin.The present invention prepares melanoidin using black garlic, and extraction time is short, extraction efficiency is high, is suitble to industrialized production.Meanwhile the melanoidin that the method for the invention is prepared has stronger oxidation resistance and blood pressure lowering ability.
Description
Technical field
The invention belongs to the preparation of biological product and processing technique fields, and in particular to the extraction of melanoidin in a kind of black garlic
Method.
Background technique
Melanoidin is formed after Maillard reaction occurs during heat treatment for the food containing reduced sugar and free amine group
It is a kind of structure is complicated, the biggish sepia polymer of molecular weight, be the main source of food color and various flavors, it is extensively
It is present in various food, it is very close with mankind's daily life.
With the improvement of living standards, the mankind also have the tendency that cumulative year after year to the intake of melanoidin.In recent years, with
Melanoidin research is goed deep into, physiological function and physiological activity also have researcher as one by wide coverage
The natural food additives of kind are applied in food industry.More and more researches show that melanoidin has antitumor, antioxygen
The physiological functions such as change, antibacterial, anti-caries tooth, hypoglycemic, blood pressure lowering.Such as the melanoidin pair in the Rhizoma Solani tuber osi fiber by heating
LS180 colon cancer cell plays the role of antiproliferative activity；Melanoidin in coffee and coffee by-product has antioxidation；Mountain
Melanoidin in western mature vinegar has significant bacteriostasis to Escherichia coli, staphylococcus aureus and bacillus subtilis.
In addition, researcher has found that melanoidin has the characteristics that inhibit amylase activity and inhibits tryptic activity, to play drop
The effect of blood glucose.Therefore, separation melanoidin is extracted, it is made to have great importance as a kind of functional food ingredients.
Black garlic is to be placed in hot and humid environment to undergo made of four alternating temperature stage post-fermentations with fresh raw garlic belt leather
Food.Compared with raw garlic, the alimentary health-care function and medical value of black garlic are enhanced, especially inoxidizability,
Cancer-resisting, bactericidal antiphlogistic, anti-aging, adjustment blood glucose blood pressure, liver protecting etc..In recent years, researcher was to black garlic
The extraction research of middle functional component is also more and more, such as extracts alliin substance.
The method that melanoidin extracts in traditional food includes water extract method, the precipitation method, macroreticular resin absorbing method, ethanol water
Solution extraction, enzymatic isolation method etc., it is generally different according to food properties and melanoidin type.However these traditional extracting methods
All there is a problem of that the reaction time is long, extraction is insufficient, product yield is unsatisfactory during the extraction process, it is big to be unfavorable for industrialization
Large-scale production.In recent years, the extraction and separation technology of Active Ingredient in Food extracted effect towards raising product yield, increase always
The direction of rate, the destruction for reducing organic reagent usage amount, reduction active component etc. is developed.In this background, it is some it is non-thermal plus
Work technology is applied to favor of the method for assisted extraction by food industry circle.High static pressure extraction is a kind of novel non-thermal technology
Technology reinforces the transport of substance using water as the medium of transmitting pressure.In extraction process, super high pressure hydraulic can instantaneously uniformly
Ground reaches material each section, at this point, foring biggish pressure difference inside and outside cell membrane, promotes solvent to enter by the film of rupture thin
Born of the same parents improve mass transfer rate, improve extraction efficiency.In addition, high static pressure may generate the structure of the macromolecular substances such as protein
It is certain to influence.
Summary of the invention
The problem of in view of background technique, the purpose of the present invention is to provide a kind of extractions of melanoidin in black garlic
Method, extraction time is short, extraction efficiency is high, is suitble to industrialized production, and the melanoidin being prepared has stronger anti-oxidant energy
Power and blood pressure lowering ability.
The present invention provides a kind of extracting methods of melanoidin in black garlic, include the following steps:
(1) black garlic of peeling is mixed with water, is ground, obtain black garlic slurry；
(2) black garlic slurry is placed under the conditions of high static pressure and carries out activation processing；
(3) by activation treated the black garlic slurry centrifugation, supernatant is obtained；
(4) ungrease treatment is carried out to the supernatant, obtains degreasing black garlic juice；
(5) dialysis treatment is carried out to the degreasing black garlic juice, dialysis molecular cut off is 12~20kDa, obtains retaining liquid；
(6) the retention liquid is dried, obtains melanoidin.
Preferably, step (1) black garlic is the purple skin single clove garlic fermentation gained that is produced from Dali Eryuan County.
Preferably, the black garlic of step (1) described peeling and the mixed proportion of water are 1g:(2~8) mL.
Preferably, the black garlic fineness of materials in step (1) the black garlic slurry is 2~40 μm.
Preferably, step (1) grinding is ground using colloid mill, and the power of the colloid mill grinding is 1~2kw, institute
The time for stating colloid mill grinding is 4~6min.
Preferably, the pressure parameter of step (2) described high static pressure is 100~500MPa, and time parameter is 5~25min.
Preferably, the centrifugal force of step (3) described centrifugation is 6000~8000g, and the time of centrifugation is 4~6min.
Preferably, step (4) described degreasing is methylene chloride with degreasing agent；The body of the methylene chloride and the supernatant
Product is than being 2:(2.5~3.5).
Preferably, the molecular cut off of step (5) the dialysis treatment bag filter is 12~20kDa；The dialysis
Time is 12~36h；The volume ratio of dialysis distilled water and the degreasing black garlic juice is 1000:(10~20)；The dialysis
Changing water spacer is 3~6h/ times.
Preferably, step (6) is described is dried to be freeze-dried, and the time of the freeze-drying is 24~60h.
The utility model has the advantages that including the following steps: that (1) will be gone the present invention provides a kind of extracting method of melanoidin in black garlic
The black garlic of skin is mixed with water, grinding, obtains black garlic slurry；(2) black garlic slurry is placed under the conditions of high static pressure and carries out activation processing；
(3) by activation treated the black garlic slurry centrifugation, supernatant is obtained；(4) ungrease treatment is carried out to the supernatant, is taken off
Rouge black garlic juice；(5) dialysis treatment is carried out to the degreasing black garlic juice, obtains retaining liquid；(6) place is dried to the retention liquid
Reason, obtains melanoidin.The present invention is using black garlic as raw material, using the melanoidin in high static pressure auxiliary extraction black garlic, when having extraction
Between it is short, extraction efficiency is high, energy conservation and environmental protection, be suitble to industrialized production processing the characteristics of.Meanwhile the effect of high static pressure can suitably change
The structure of melanoidin macromolecular improves its physiological activity, and the melanoidin being prepared has stronger oxidation resistance and drop blood
Pressure energy power.The present invention for prepare the novel natural food additive melanoidin for being suitble to Chinese residents edible provide new thinking and
Method.
Specific embodiment
The present invention provides a kind of extracting methods of melanoidin in black garlic, include the following steps:
(1) black garlic of peeling is mixed with water, is ground, obtain black garlic slurry；
(2) black garlic slurry is placed under the conditions of high static pressure and carries out activation processing；
(3) by activation treated the black garlic slurry centrifugation, supernatant is obtained；
(4) ungrease treatment is carried out to the supernatant, obtains degreasing black garlic juice；
(5) dialysis treatment is carried out to the degreasing black garlic juice, dialysis molecular cut off is 12~20kDa, obtains retaining liquid；
(6) the retention liquid is dried, obtains melanoidin.
The present invention prepares raw material using black garlic as melanoidin.In the present invention, the black garlic is preferably Dali Pu'er tea
Obtained by the purple skin single clove garlic that is produced from source county ferments, more preferably purchase is produced from the Dali plateau Pin Hong agricultural science and technology development corporation, Ltd.
Purple Pi Dutou black garlic.During the fermentation, color becomes black by milky to black garlic, and this is mainly due to present in raw garlic
A large amount of substance reactions such as reduced sugar and protein, amino acid generate a large amount of melanoidin.The present invention is using black garlic as extraction class
The raw material of black essence, the yield of melanoidin more horn of plenty.
The present invention mixes the black garlic of peeling with water, grinding, obtains black garlic slurry.In the present invention, the black garlic of the peeling
Mixed proportion with water is preferably 1g:(2~8) mL, more preferably 1g:(3~6) mL, more preferably 1g:3mL.The present invention limits
Black garlic and the mixed proportion of water can promote the recovery rate of melanoidin to the greatest extent, while will not additionally increase dialysis, freeze-drying
Cost.In the present invention, the grinding is preferably ground using colloid mill, and the power of the colloid mill grinding is preferably 1~2kw,
More preferably 1.5kw；The time of the colloid mill grinding is preferably 4~6min, more preferably 5min.It is obtained after the grinding black
Garlic slurry, in the present invention, black garlic fineness of materials in black garlic slurry preferably≤40 μm, more preferably 2~20 μm.
After obtaining black garlic slurry, black garlic slurry is placed under the conditions of high static pressure and carries out activation processing by the present invention.In the present invention
In, the pressure parameter of the high static pressure is preferably 100~500MPa, more preferably 200~300MPa.The processing of the high static pressure
Time is preferably 5~25min, more preferably 15~20min.High static pressure processing can to black garlic starch in melanoidin non-covalent bond,
Such as hydrogen bond, ionic bond and hydrophobic bond are had an impact, and can also change protein by the protein chain in expansion melanoidin
Structure is to enhance its physiological activity, such as inoxidizability, blood pressure lowering ability etc..
After activation processing, activation treated the black garlic slurry centrifugation is obtained supernatant by the present invention.In the present invention,
The centrifugal force of the centrifugation is preferably 6000~8000g, more preferably 7000g；The time of the centrifugation is preferably 4~6min,
More preferably 5min.The present invention preferably filters the supernatant obtained after the centrifugation, and described filter with filter paper is qualitative
Filter paper, filtering velocity are middling speed, and vacuum degree is -0.1MPa.
After obtaining supernatant, the present invention carries out ungrease treatment to the supernatant, obtains degreasing black garlic juice.In the present invention
In, the degreasing is preferably methylene chloride with degreasing agent；The volume ratio of the methylene chloride and the supernatant is preferably 2:
(2.5~3.5), more preferably 2:3.In the present invention, the ungrease treatment is preferably once repeated, to reach preferably de-
Rouge effect.
After obtaining degreasing black garlic juice, the present invention carries out dialysis treatment to the degreasing black garlic juice.In the present invention, described
The molecular cut off for analysing processing bag filter is preferably 12~20kDa；The time of the dialysis is preferably 12~36h, more preferably
For for 24 hours；The volume ratio of dialysis distilled water and the degreasing black garlic juice is preferably 1000:(10~20), more preferably 1000:
15；The water spacer that changes of the dialysis is preferably 3~6h/ times.
After dialysis, obtain retaining liquid.The retention liquid is dried in the present invention, obtains melanoidin.In the present invention
In, the drying process preferably uses freeze-drying, and the time of the freeze-drying is preferably 24~60h, more preferably
48h。
Technical solution provided by the invention is described in detail below with reference to embodiment, but they cannot be understood
For limiting the scope of the present invention.In embodiment of the invention every, the unit of the solid-liquid ratio unless otherwise instructed,
For g/mL.
Embodiment 1
The present embodiment is related to a kind of extracting method of 12kDa melanoidin in black garlic, includes the following steps:
Step A, black garlic is removed the peel, and is carried out with solid-liquid ratio 1:3 plus water mixing, colloid mill grind 5min, high static pressure 200MPa
Handle 5min.
Step B, resulting black garlic slurry is centrifuged 5min under the conditions of 7000g after high static pressure is handled, and filters, uses methylene chloride
Twice, methylene chloride: black garlic juice is 2:3 (V/V) for degreasing.
Step C is dialysed the black garlic juice after degreasing for 24 hours with the bag filter that molecular cut off is 12kDa, freeze-drying
48h obtains macromolecular melanoidin.
Embodiment 2
The present embodiment is related to a kind of extracting method of 12kDa melanoidin in black garlic, includes the following steps:
Step A, black garlic is removed the peel, and is carried out with solid-liquid ratio 1:3 plus water mixing, colloid mill grind 5min, high static pressure 300MPa
Handle 5min.
Step B, C is the same as embodiment 1.
Embodiment 3
The present embodiment is related to a kind of extracting method of 12kDa melanoidin in black garlic, includes the following steps:
Step A, black garlic is removed the peel, and is carried out with solid-liquid ratio 1:3 plus water mixing, colloid mill grind 5min, high static pressure 400MPa
Handle 5min.
Step B, C is the same as embodiment 1.
Embodiment 4
The present embodiment is related to a kind of extracting method of 12kDa melanoidin in black garlic, includes the following steps:
Step A, black garlic is removed the peel, and is carried out with solid-liquid ratio 1:3 plus water mixing, colloid mill grind 5min, high static pressure 500MPa
Handle 5min.
Step B, C is the same as embodiment 1.
Embodiment 5
The present embodiment is related to a kind of extracting method of 12kDa melanoidin in black garlic, includes the following steps:
Step A, black garlic is removed the peel, and is carried out with solid-liquid ratio 1:3 plus water mixing, colloid mill grind 5min, high static pressure 200MPa
Handle 10min.
Step B, C is the same as embodiment 1.
Embodiment 6
The present embodiment is related to a kind of extracting method of 12kDa melanoidin in black garlic, includes the following steps:
Step A, black garlic is removed the peel, and is carried out with solid-liquid ratio 1:3 plus water mixing, colloid mill grind 5min, high static pressure 200MPa
Handle 15min.
Step B, C is the same as embodiment 1.
Embodiment 7
The present embodiment is related to a kind of extracting method of 12kDa melanoidin in black garlic, includes the following steps:
Step A, black garlic is removed the peel, and is carried out with solid-liquid ratio 1:3 plus water mixing, colloid mill grind 5min, high static pressure 200MPa
Handle 20min.
Step B, C is the same as embodiment 1.
Embodiment 8
The present embodiment is related to a kind of extracting method of 12kDa melanoidin in black garlic, includes the following steps:
Step A, black garlic is removed the peel, and is carried out with solid-liquid ratio 1:3 plus water mixing, colloid mill grind 5min, high static pressure 200MPa
Handle 25min.
Step B, C is the same as embodiment 1.
Embodiment 9
The present embodiment is related to a kind of extracting method of 12kDa melanoidin in black garlic, includes the following steps:
Step A, black garlic is removed the peel, and is carried out with solid-liquid ratio 1:6 plus water mixing, colloid mill grind 5min, high static pressure 200MPa
Handle 5min.
Step B, C is the same as embodiment 1.
Comparative example 1
This comparative example is related to a kind of extracting method of 12kDa melanoidin in black garlic, and step is substantially the same as embodiment 1, difference
It is that this comparative example, without high static pressure processing, directly carries out step B, C after colloid mill grinding when carrying out water and mentioning.
Comparative example 2
This comparative example is related to a kind of extracting method of 12kDa melanoidin in black garlic, and step is substantially the same as embodiment 1, difference
It is that this comparative example after black garlic peeling, cuts fritter, is carried out with solid-liquid ratio 1:3 plus 5min, then high static pressure is mixed in water
Step B, C is directly carried out after 200MPa processing 5min.
The analysis experiment of melanoidin extraction effect
(1) 280,325, specific extinction coefficient measurement experiment at 405nm: the class that Example 1-9 and comparative example 1 obtain respectively
Black essence sample, is made into the aqueous solution of 1.5mg/mL, measures absorbance under ultraviolet specrophotometer 280,325,405nm wavelength,
Specific extinction coefficient is calculated, K is denoted asmix280nm、Kmix325nm、Kmix405nm。
(2) DPPH free radical scavenging ability is tested: the melanoidin sample that Example 1-9 and comparative example 1 obtain respectively is matched
At the aqueous solution of 5mg/mL, the 100 μ L solution are taken, the DPPH ethanol solution of 900 μ L, 0.1mmol/L is added, and (volume fraction is
95%) it, mixes, is protected from light 30min, measures absorbance under microplate reader 517nm wavelength.Simultaneously measure 100 μ L distilled water with
Light absorption value after the DPPH ethanol solution reaction of 900 μ L, 0.1mmol/L.
(3) ABTS free radical scavenging ability test: configure certain volume ABTS mixed liquor (7mmol/LABTS with
The mixing of 2.45mmo/L potassium persulfate solution 1:1 volume ratio, is protected from light 12h).With 5mmol/L, the phosphate buffer solution of pH=7
ABTS mixed liquor is diluted, makes its light absorption value 0.7 ± 0.02 at 734nm, obtains ABTS working solution.
The melanoidin sample that Example 1-9 and comparative example 1 obtain respectively, is made into the aqueous solution of 5mg/mL, takes 20 μ L should
980 μ LABTS working solutions are added in solution, mix, and react 6min.With the absorbance of liquid after reaction at microplate reader measurement 734nm.
The light absorption value after 20 μ L distilled water are reacted with 980 μ L ABTS working solutions is measured simultaneously.
(4) reducing power measures: the melanoidin sample that Example 1-9 and comparative example 1 obtain respectively is made into 5mg/mL's
Aqueous solution takes the 100 μ L solution, and the phosphate buffer solution and 250 μ L of 250 μ L, 0.2mol/L, pH=6.6 is added, 10g/L's
Potassium ferricyanide solution mixes.The solution of trichloroacetic acid of 250 μ L, 0.1kg/L, reaction is added in 50 DEG C of water-bath 20min after cooling
10min.250 μ L mixed liquors are taken, the ferric chloride solution of 250 μ L distilled water and 50 μ L, 1.0g/L is added, mixes and stands 10min, are used
Microplate reader measures its light absorption value at 700nm wavelength.
(5) blood pressure lowering ability measures: ACE Kit-WST (A502) kit is purchased from the limited public affairs of eastern Renhua subject skill (Shanghai)
Department.
It is as follows that melanoidin extraction effect analyzes experimental result:
1 embodiment 1-9 of table and 1 gained melanoidin of the comparative example specific extinction coefficient at 280,325,405nm
| Kmix280nm(L/g/cm) | Kmix325nm(L/g/cm) | Kmix405nm(L/g/cm) | |
| Embodiment 1 | 2.360 | 1.711 | 0.728 |
| Embodiment 2 | 2.576 | 2.216 | 0.840 |
| Embodiment 3 | 2.584 | 1.897 | 0.804 |
| Embodiment 4 | 2.462 | 1.787 | 0.753 |
| Embodiment 5 | 2.336 | 1.689 | 0.716 |
| Embodiment 6 | 2.420 | 1.737 | 0.743 |
| Embodiment 7 | 2.349 | 1.703 | 0.723 |
| Embodiment 8 | 2.493 | 1.800 | 0.760 |
| Embodiment 9 | 2.436 | 1.733 | 0.707 |
| Comparative example 1 | 1.477 | 1.046 | 0.469 |
So that it has absorbance at 280nm, the presence of phenolic acid makes it at 325nm for the presence meeting of albumen in melanoidin
There is absorbance.In addition, when detection melanoidin content, it will usually which selection measures its absorbance at 405nm wavelength.When gained produces
Melanoidin concrete content and specific extinction coefficient K when unknown molal weight, under specific wavelength in objectmixIt can provide about albumen
Matter histone (Kmix405nm) and the melanoidin that is formed as black garlic present in other compounds such as protein (Kmix280nm) and phenolic acid
(Kmix280,325nm) relative quantity information.
By Examples 1 to 9 and comparative example 1 it is found that high static pressure processing can have an impact macromolecular substances structure, thus aobvious
Write the relative amount for improving melanoidin in products therefrom.
It is found in 2 experimentation of comparative example, the eduction rate of the black garlic being only cut into small pieces melanoidin in water is very low, and gained is black
Garlic juice is of light color, and the result that can not be ground with colloid mill is compared.
Gained melanoidin physiological activity comparison in 2 embodiment 1-9 of table and comparative example 1
| DPPH% | ABTS% | Reducing power % | Blood pressure lowering ability % | |
| Embodiment 1 | 42.3 | 49.1 | 48.9 | 45.9 |
| Embodiment 2 | 40.0 | 52.9 | 40.8 | 50.6 |
| Embodiment 3 | 31.0 | 39.7 | 34.2 | 49.0 |
| Embodiment 4 | 42.3 | 54.2 | 48.4 | 56.2 |
| Embodiment 5 | 45.6 | 49.2 | 53.2 | 54.5 |
| Embodiment 6 | 45.6 | 51.1 | 50.0 | 53.4 |
| Embodiment 7 | 43.7 | 48.6 | 51.1 | 55.2 |
| Embodiment 8 | 44.4 | 46.3 | 47.6 | 56.6 |
| Embodiment 9 | 36.8 | 25.4 | 30.0 | 56.0 |
| Comparative example 1 | 32.7 | 29.6 | 29.0 | 36.9 |
Influence in order to further determine high static pressure to melanoidin physiological activity, the present invention is to its oxidation resistance and drop blood
Pressure energy power is determined.The result shows that: high static pressure processing can significantly improve the antioxygenic property and blood pressure lowering energy of melanoidin
Power.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. the extracting method of melanoidin in a kind of black garlic, which comprises the steps of:
(1) black garlic of peeling is mixed with water, is ground, obtain black garlic slurry；
(2) black garlic slurry is placed under the conditions of high static pressure and carries out activation processing；
(3) by activation treated the black garlic slurry centrifugation, supernatant is obtained；
(4) ungrease treatment is carried out to the supernatant, obtains degreasing black garlic juice；
(5) dialysis treatment is carried out to the degreasing black garlic juice, dialysis molecular cut off is 12~20kDa, obtains retaining liquid；
(6) the retention liquid is dried, obtains melanoidin.
2. extracting method according to claim 1, which is characterized in that step (1) black garlic is Dali Eryuan County
Obtained by the purple skin single clove garlic of production ferments.
3. extracting method according to claim 1 or 2, which is characterized in that the black garlic of step (1) described peeling and mixing for water
Composition and division in a proportion example is 1g:(2~8) mL.
4. extracting method according to claim 1, which is characterized in that the black garlic material in step (1) the black garlic slurry is thin
Degree is 2~40 μm.
5. extracting method according to claim 1 or 4, which is characterized in that step (1) grinding uses colloid barreling
The power of mill, the colloid mill grinding is 1~2kw, and the time of the colloid mill grinding is 4~6min.
6. extracting method according to claim 1, which is characterized in that the pressure parameter of step (2) described high static pressure is 100
~500MPa, time parameter are 5~25min.
7. extracting method according to claim 1, which is characterized in that the centrifugal force of step (3) described centrifugation be 6000~
8000g, the time of centrifugation are 4~6min.
8. extracting method according to claim 1, which is characterized in that step (4) described degreasing is dichloromethane with degreasing agent
Alkane；The volume ratio of the methylene chloride and the supernatant is 2:(2.5~3.5).
9. extracting method according to claim 1, which is characterized in that step (5) the dialysis treatment bag filter is cut
Staying molecular weight is 12~20kDa；The time of the dialysis is 12~36h；The dialysis body of distilled water and the degreasing black garlic juice
Product is than being 1000:(10~20)；The water spacer that changes of the dialysis is 3~6h/ times.
10. extracting method according to claim 1, which is characterized in that step (6) is described to be dried to be freeze-dried,
The time of the freeze-drying is 24~60h.
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| CN110214953A (en) * | 2019-07-01 | 2019-09-10 | 天津理工大学 | The separation method of the melanoidin ingredient of different molecular weight in a kind of black garlic |
| CN112826092A (en) * | 2019-11-22 | 2021-05-25 | 中国科学院大连化学物理研究所 | Pectin type melanoidin and preparation method thereof |
| CN116022964A (en) * | 2023-01-06 | 2023-04-28 | 北京工业大学 | Method for extracting melanoidin from thermal hydrolysis sludge by using macroporous resin |
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| CN108967955A (en) * | 2018-08-01 | 2018-12-11 | 山东农业大学 | The extracting method of melanoidin in a kind of blacking jujube |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110214953A (en) * | 2019-07-01 | 2019-09-10 | 天津理工大学 | The separation method of the melanoidin ingredient of different molecular weight in a kind of black garlic |
| CN112826092A (en) * | 2019-11-22 | 2021-05-25 | 中国科学院大连化学物理研究所 | Pectin type melanoidin and preparation method thereof |
| CN116022964A (en) * | 2023-01-06 | 2023-04-28 | 北京工业大学 | Method for extracting melanoidin from thermal hydrolysis sludge by using macroporous resin |
| CN116022964B (en) * | 2023-01-06 | 2024-06-18 | 北京工业大学 | Method for extracting melanoidin from thermal hydrolysis sludge by using macroporous resin |
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Application publication date: 20190514 |