CN113559236A - Selenium-rich walnut and dendrobium composition, tablet and application thereof - Google Patents
Selenium-rich walnut and dendrobium composition, tablet and application thereof Download PDFInfo
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- CN113559236A CN113559236A CN202110911648.4A CN202110911648A CN113559236A CN 113559236 A CN113559236 A CN 113559236A CN 202110911648 A CN202110911648 A CN 202110911648A CN 113559236 A CN113559236 A CN 113559236A
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- 239000011669 selenium Substances 0.000 title claims abstract description 202
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 201
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 201
- 235000020234 walnut Nutrition 0.000 title claims abstract description 115
- 241001523681 Dendrobium Species 0.000 title claims abstract description 64
- 239000000203 mixture Substances 0.000 title claims abstract description 25
- 235000009496 Juglans regia Nutrition 0.000 title claims description 101
- 240000007049 Juglans regia Species 0.000 title 1
- 229920001184 polypeptide Polymers 0.000 claims abstract description 48
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 48
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 48
- 230000000694 effects Effects 0.000 claims abstract description 13
- 230000003078 antioxidant effect Effects 0.000 claims abstract description 11
- 239000003963 antioxidant agent Substances 0.000 claims abstract description 6
- 229940079593 drug Drugs 0.000 claims abstract description 4
- 239000003814 drug Substances 0.000 claims abstract description 4
- 241000758789 Juglans Species 0.000 claims description 100
- 239000000843 powder Substances 0.000 claims description 57
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 37
- 238000002156 mixing Methods 0.000 claims description 22
- 102000004169 proteins and genes Human genes 0.000 claims description 20
- 108090000623 proteins and genes Proteins 0.000 claims description 20
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 claims description 18
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 17
- 238000001035 drying Methods 0.000 claims description 11
- 235000019359 magnesium stearate Nutrition 0.000 claims description 9
- 239000005913 Maltodextrin Substances 0.000 claims description 8
- 229920002774 Maltodextrin Polymers 0.000 claims description 8
- UEDUENGHJMELGK-HYDKPPNVSA-N Stevioside Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O UEDUENGHJMELGK-HYDKPPNVSA-N 0.000 claims description 8
- 229940035034 maltodextrin Drugs 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 8
- 229940013618 stevioside Drugs 0.000 claims description 8
- OHHNJQXIOPOJSC-UHFFFAOYSA-N stevioside Natural products CC1(CCCC2(C)C3(C)CCC4(CC3(CCC12C)CC4=C)OC5OC(CO)C(O)C(O)C5OC6OC(CO)C(O)C(O)C6O)C(=O)OC7OC(CO)C(O)C(O)C7O OHHNJQXIOPOJSC-UHFFFAOYSA-N 0.000 claims description 8
- 235000019202 steviosides Nutrition 0.000 claims description 8
- 102000012479 Serine Proteases Human genes 0.000 claims description 7
- 108010022999 Serine Proteases Proteins 0.000 claims description 7
- 239000003513 alkali Substances 0.000 claims description 7
- 238000007605 air drying Methods 0.000 claims description 6
- 235000019498 Walnut oil Nutrition 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 150000002632 lipids Chemical class 0.000 claims description 5
- 235000012054 meals Nutrition 0.000 claims description 5
- 239000008170 walnut oil Substances 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 4
- 230000036541 health Effects 0.000 claims description 2
- 239000002245 particle Substances 0.000 claims description 2
- 241000758791 Juglandaceae Species 0.000 abstract description 14
- 238000010521 absorption reaction Methods 0.000 abstract description 4
- 230000003712 anti-aging effect Effects 0.000 abstract description 4
- 238000000605 extraction Methods 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 230000036425 denaturation Effects 0.000 abstract description 3
- 238000004925 denaturation Methods 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
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- 238000012545 processing Methods 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 2
- 229940091258 selenium supplement Drugs 0.000 description 172
- 239000000243 solution Substances 0.000 description 24
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 18
- 239000000047 product Substances 0.000 description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 14
- 239000012153 distilled water Substances 0.000 description 14
- 239000012488 sample solution Substances 0.000 description 14
- 238000002835 absorbance Methods 0.000 description 13
- 150000004676 glycans Chemical class 0.000 description 11
- 229920001282 polysaccharide Polymers 0.000 description 11
- 239000005017 polysaccharide Substances 0.000 description 11
- 238000000034 method Methods 0.000 description 9
- 239000000523 sample Substances 0.000 description 9
- 241001076416 Dendrobium tosaense Species 0.000 description 7
- 150000003254 radicals Chemical class 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 230000003647 oxidation Effects 0.000 description 6
- 238000007254 oxidation reaction Methods 0.000 description 6
- 230000002292 Radical scavenging effect Effects 0.000 description 5
- 239000003337 fertilizer Substances 0.000 description 5
- 238000007873 sieving Methods 0.000 description 5
- PMYDPQQPEAYXKD-UHFFFAOYSA-N 3-hydroxy-n-naphthalen-2-ylnaphthalene-2-carboxamide Chemical group C1=CC=CC2=CC(NC(=O)C3=CC4=CC=CC=C4C=C3O)=CC=C21 PMYDPQQPEAYXKD-UHFFFAOYSA-N 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 4
- MHAJPDPJQMAIIY-UHFFFAOYSA-N hydrogen peroxide Substances OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
- -1 hydroxyl radicals Chemical class 0.000 description 4
- 230000001965 increasing effect Effects 0.000 description 4
- 230000031700 light absorption Effects 0.000 description 4
- 239000013642 negative control Substances 0.000 description 4
- 239000008213 purified water Substances 0.000 description 4
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 4
- 229960001881 sodium selenate Drugs 0.000 description 4
- 239000011655 sodium selenate Substances 0.000 description 4
- 235000018716 sodium selenate Nutrition 0.000 description 4
- 238000005507 spraying Methods 0.000 description 4
- 235000013305 food Nutrition 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000001694 spray drying Methods 0.000 description 3
- DGEZNRSVGBDHLK-UHFFFAOYSA-N [1,10]phenanthroline Chemical compound C1=CN=C2C3=NC=CC=C3C=CC2=C1 DGEZNRSVGBDHLK-UHFFFAOYSA-N 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 235000013361 beverage Nutrition 0.000 description 2
- 230000007760 free radical scavenging Effects 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 238000002386 leaching Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 235000019198 oils Nutrition 0.000 description 2
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 229960004889 salicylic acid Drugs 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 244000144730 Amygdalus persica Species 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 240000004638 Dendrobium nobile Species 0.000 description 1
- 102000006587 Glutathione peroxidase Human genes 0.000 description 1
- 108700016172 Glutathione peroxidases Proteins 0.000 description 1
- 241000893545 Liquidambar formosana Species 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000008425 Protein deficiency Diseases 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 206010039921 Selenium deficiency Diseases 0.000 description 1
- 102000008114 Selenoproteins Human genes 0.000 description 1
- 108010074686 Selenoproteins Proteins 0.000 description 1
- 238000000944 Soxhlet extraction Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 230000001071 malnutrition Effects 0.000 description 1
- 235000000824 malnutrition Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 208000015380 nutritional deficiency disease Diseases 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000009758 senescence Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
- A61K36/8984—Dendrobium
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/011—Hydrolysed proteins; Derivatives thereof from plants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
- A61K9/2004—Excipients; Inactive ingredients
- A61K9/2013—Organic compounds, e.g. phospholipids, fats
- A61K9/2018—Sugars, or sugar alcohols, e.g. lactose, mannitol; Derivatives thereof, e.g. polysorbates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
- A61K9/2004—Excipients; Inactive ingredients
- A61K9/2022—Organic macromolecular compounds
- A61K9/205—Polysaccharides, e.g. alginate, gums; Cyclodextrin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/52—Juglandaceae (Walnut family)
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- General Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
- Biochemistry (AREA)
- Toxicology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
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Abstract
The invention relates to a selenium-rich walnut-dendrobium composition, a selenium-rich walnut-dendrobium tablet and application thereof, belongs to the technical field of biological medicines, effectively improves the content of organic selenium in a product by controlling walnuts and dendrobium, adopts the technologies of cold pressing and low-temperature extraction in the production process, prevents the denaturation of the organic selenium under the high-temperature processing condition, improves the selenium content of unit weight of the prepared selenium-rich walnut-dendrobium tablet, has stronger selenium polypeptide activity, is more beneficial to human body absorption, and has good antioxidant and anti-aging effects.
Description
Technical Field
The invention relates to the technical field of biomedicine, and particularly relates to a selenium-rich walnut-dendrobium composition, a selenium-rich walnut-dendrobium tablet and application thereof.
Background
Selenium is one of essential trace elements for human body, and research shows that selenium has therapeutic effect on children with malnutrition caused by protein deficiency and has resistance effect on some possible carcinogenic substances. The selenium-rich product is the main source of selenium supplement, and the selenium element is used as the essential component of glutathione peroxidase, plays roles of protecting cell membranes, resisting cancers, delaying senescence, preventing cardiovascular and cerebrovascular diseases and the like in a human body, and is closely related to the special health care efficacy of the selenium-rich product. With the increasingly clear understanding of the problem of general selenium deficiency in the world, how to scientifically, reasonably, healthily and safely supplement selenium is a great concern. In recent years, related researches on selenium in natural or artificial selenium-rich resources are gradually increased at home and abroad, but the problems of oxidation resistance and the like of selenium-rich products are not effectively solved.
In order to improve the oxidation resistance of selenium-rich products and the like, various selenium-rich products exist in the prior art, for example, the selenium-rich special meal beverage prepared from various selenium-rich raw materials such as selenium-rich peanuts, selenium-rich walnut powder and the like has the function of removing free radicals in vivo in the invention patent application with the application publication number of CN 111066892A. In another example, in the invention patent application with application publication number CN105831536A, the compound beverage prepared from the selenium-rich dendrobe juice has the functions of reducing blood fat and enhancing immunity. However, the existing selenium-rich walnuts mainly contain dry fruits, the selenium enrichment degree is general, the activation degree of selenoprotein in the walnut kernels is not high, the selenium-rich walnut kernels cannot be quickly absorbed by human bodies, the selenium-rich dendrobium stems mainly contain Chinese sweetgum fruits, the absorption effectiveness is poor, and related records of compounding the selenium-rich walnuts and the selenium-rich dendrobium stem are not provided.
Disclosure of Invention
In order to solve the defects of the prior art, the invention aims to provide the selenium-rich walnut and dendrobium composition, wherein selenium-rich walnuts and selenium-rich dendrobium are compounded, so that the selenium content of unit weight is increased, the selenium polypeptide activity is stronger, the oxidation resistance of the product is obviously improved, and the selenium-rich walnut and dendrobium composition has good oxidation resistance and anti-aging effects.
In order to solve the technical problems, the invention provides the following technical scheme:
the invention provides a selenium-rich walnut-dendrobium composition which comprises 25-35 parts by weight of walnut selenium polypeptide dry powder and 15-25 parts by weight of selenium-rich dendrobium superfine powder.
Preferably, the preparation of the walnut selenium polypeptide dry powder comprises the following steps:
1) squeezing selenium-rich walnut to obtain walnut oil and cake;
2) mixing the cake with ether to extract lipid to obtain defatted walnut cake;
3) air-drying the degreased walnut cake, and mixing with alkali liquor to extract protein;
4) mixing the extracted protein with serine protease for enzymolysis reaction, and drying the enzymolysis product to obtain the walnut selenium polypeptide dry powder.
Preferably, the mass ratio of the cake dregs to the diethyl ether in the step 2) is 2.5: 1-1.5: 1.
preferably, the mass-to-volume ratio of the defatted walnut cake meal to the alkali liquor in the step 3) is 18: 1-23: 1, the temperature for extracting the protein is 50-60 ℃, and the pH value is 8.0-9.5.
Preferably, the protein extracted in the step 3) is dried and then uniformly mixed with water, and the mass percentage of the protein in the water is 25-35%.
Preferably, the enzyme activity of the serine protease in the step 4) is more than or equal to 4000U/g.
Preferably, the selenium content of the walnut selenium polypeptide dry powder is more than or equal to 1.5mg/kg, and the selenium content of the selenium-rich dendrobium superfine powder is more than or equal to 2 mg/kg.
Preferably, the grain size of the selenium-rich dendrobium superfine powder is less than or equal to 38 mu m.
The invention provides a selenium-rich walnut dendrobium tablet, which comprises the following raw materials: 25-35 parts of walnut selenium polypeptide dry powder, 15-25 parts of selenium-enriched dendrobe superfine powder, 2-5 parts of stevioside, 0.2-0.5 part of magnesium stearate, 20-30 parts of maltodextrin and 30-40 parts of pure water.
The invention also provides an application of the selenium-rich walnut-dendrobe composition or the walnut-dendrobe tablet in preparation of an antioxidant drug or a health-care product.
The selenium-rich walnut and dendrobe composition disclosed by the invention takes walnut selenium polypeptide dry powder and selenium-rich dendrobe superfine powder as raw materials, and the walnut selenium polypeptide and the selenium-rich dendrobe powder are compounded, so that a synergistic effect is achieved, and the oxidation resistance is obviously improved. According to the selenium-enriched walnut dendrobium tablet, the organic selenium content in the product is effectively improved through cultivation of walnut and dendrobium raw materials, the production process adopts the cold pressing and low-temperature extraction technology, the denaturation of the organic selenium under the high-temperature processing condition is prevented, the selenium content of the prepared selenium-enriched walnut dendrobium tablet is improved, the selenium polypeptide activity is stronger, the selenium polypeptide activity is more beneficial to human body absorption, and the selenium-enriched walnut dendrobium tablet has good antioxidant and anti-aging effects.
Detailed Description
The invention provides a selenium-rich walnut-dendrobium composition which comprises 25-35 parts by weight of walnut selenium polypeptide dry powder and 15-25 parts by weight of selenium-rich dendrobium superfine powder.
The selenium-rich walnut dendrobium composition comprises 25-35 parts by weight of walnut selenium polypeptide dry powder, preferably 28-32 parts by weight, and more preferably 30 parts by weight. The selenium content in the walnut selenium polypeptide dry powder is preferably more than or equal to 1.5mg/kg, and more preferably more than or equal to 2 mg/kg. In the invention, the walnut selenium polypeptide dry powder is prepared from selenium-rich walnuts. The source of the selenium-rich walnuts is not particularly limited, in a specific embodiment of the invention, the selenium-rich walnuts are preferably cultivated by using a special selenium fertilizer, and the selenium-rich walnuts cultivating step comprises the following steps: applying a special selenium fertilizer in the growth process of walnuts, harvesting mature walnuts in the current year, drying and then determining the selenium content.
The selenium-rich walnut dendrobium composition comprises 15-25 parts by weight of selenium-rich dendrobium superfine powder, preferably 18-23 parts by weight, and more preferably 20 parts by weight. The content of selenium in the selenium-rich dendrobium superfine powder is preferably more than or equal to 2mg/kg, and more preferably more than or equal to 5 mg/kg. In the invention, the selenium-rich dendrobium superfine powder is obtained by superfine grinding and sieving of selenium-rich dendrobium, and the particle size of the selenium-rich dendrobium superfine powder is preferably less than or equal to 38 micrometers. The invention is not specially limited for the source of the selenium-rich dendrobium, in the specific embodiment of the invention, the selenium-rich dendrobium is preferably cultivated by using a special selenium fertilizer, and the cultivation of the selenium-rich dendrobium comprises the following steps: applying a special selenium fertilizer in the growth process of the dendrobium, and measuring the selenium content after drying fresh dendrobium strips harvested in the same year.
In the invention, the method for measuring the selenium content is preferably measured according to GB 5009.93-2010 national standard food for food safety 'determination of selenium'. In the invention, the special selenium fertilizer is preferably sodium selenate, the sodium selenate is preferably 0.1-1mg/kg aqueous solution, the sodium selenate is preferably applied by spraying, and the spraying frequency is preferably 1-4 times/day.
In the invention, the preparation of the walnut selenium polypeptide dry powder comprises the following steps:
1) squeezing selenium-rich walnut to obtain walnut oil and cake;
2) mixing the cake with ether to extract lipid to obtain defatted walnut cake;
3) air-drying the degreased walnut cake, and mixing with alkali liquor to extract protein;
4) mixing the extracted protein with serine protease for enzymolysis reaction, and drying the enzymolysis product to obtain the walnut selenium polypeptide dry powder.
In the invention, the squeezing temperature is preferably 50-70 ℃, more preferably 55-65 ℃, and the oil yield of the walnut oil obtained by squeezing is preferably more than 50%. In the invention, the squeezing adopts a cold squeezing method, so that the damage of the structure of organic selenium in the selenium-rich walnuts is avoided, the content of the organic selenium in the selenium-rich walnuts is ensured, and the selenium content in the selenium-rich walnut-dendrobium composition is favorably improved.
In the invention, the mass ratio of the cake meal to the diethyl ether is preferably 2.5: 1-1.5: 1, more preferably 2: 1. In the present invention, the method for extracting lipids is preferably a Soxhlet extraction method. In the present invention, the temperature of the air-drying is preferably 20 to 50 ℃, more preferably 30 to 45 ℃. In the invention, the mass volume ratio of the degreased walnut cake and the alkali liquor is preferably 18: 1-23: 1, more preferably 20: 1. In the present invention, the alkali solution is preferably a sodium hydroxide solution, the temperature of the extracted protein is preferably 50 to 60 ℃, more preferably 55 ℃, and the pH of the extracted protein is preferably 8.0 to 9.5, more preferably 9.0.
In the present invention, the protein obtained by the extraction is preferably dried and dissolved. In the present invention, the drying temperature is preferably 40 to 65 ℃, more preferably 50 to 60 ℃, the drying method is not particularly limited, and the drying method in the embodiment of the present invention is preferably spray drying. In the present invention, the mass percentage of the protein in water at the time of dissolution is preferably 25 to 35%, more preferably 30%.
In the invention, the enzymolysis temperature is preferably 40-50 ℃, and more preferably 45 ℃; the pH of the enzymatic hydrolysis is preferably 9-11, more preferably 10. In the invention, the enzyme activity of the serine protease is preferably more than or equal to 4000U/g.
The invention provides a selenium-rich walnut dendrobium tablet, which comprises the following raw materials: 25-35 parts of walnut selenium polypeptide dry powder, 15-25 parts of selenium-enriched dendrobe superfine powder, 2-5 parts of stevioside, 0.2-0.5 part of magnesium stearate, 20-30 parts of maltodextrin and 30-40 parts of pure water.
The selenium-rich walnut dendrobium tablet comprises 2-5 parts by weight of stevioside, preferably 3-4 parts by weight. The selenium-rich walnut dendrobium tablet comprises 0.2-0.5 part by weight of magnesium stearate, preferably 0.3-0.4 part by weight of magnesium stearate. The selenium-rich walnut dendrobium tablet comprises 20-30 parts by weight of maltodextrin, preferably 23-25 parts by weight. The selenium-rich walnut dendrobium tablet comprises 30-40 parts by weight of pure water, preferably 34-38 parts by weight. According to the invention, the walnut selenium polypeptide dry powder and the selenium-rich dendrobe superfine powder are used as functional components, stevioside is used as a sweetening agent, magnesium stearate is used as a lubricant and an anti-sticking agent, and maltodextrin is used as a thickening agent, so that the prepared tablet combines the walnut selenium polypeptide and the selenium-rich dendrobe powder, the selenium content of unit weight is improved, the selenium polypeptide activity is stronger, the bioavailability is good, and the quality is stable.
The invention also provides an application of the selenium-rich walnut-dendrobe composition or the walnut-dendrobe tablet in preparation of an antioxidant drug or a health-care product.
In order to further illustrate the present invention, the following embodiments are described in detail, but they should not be construed as limiting the scope of the present invention.
Example 1
Preparation of selenium-rich walnut-dendrobium composition
1. Raw material cultivation detection
Spraying 0.1-1mg/kg sodium selenate aqueous solution for 5-8 months, respectively, and spraying 1-4 times per day. After the walnut is ripe, the walnut is collected, the outer skin of the walnut is mechanically removed, and the walnut is dried in the sun for 48 hours. Cutting fresh branches of herba Dendrobii with scissors, cleaning with leaves, and air drying at 50 deg.C. And then respectively measuring the selenium content in the dried walnut fruits and the air-dried dendrobium leaves according to a method in GB 5009.93-2010 national standard food for measuring selenium, wherein the selenium content in the walnut fruits is 1.5mg/kg, and the selenium content in the dendrobium leaves is 2 mg/kg.
2. Preparation of walnut selenium polypeptide and selenium-rich dendrobium powder
A) The walnut oil in the selenium-rich walnuts is squeezed by a cold squeezing method at 50 ℃, and the oil yield is ensured to be more than 50%. And mixing the cold-pressed cake with ether at a mass-volume ratio of 2:1 to extract lipid to obtain the defatted walnut cake. And (2) placing the degreased walnut cake in a low-temperature 30 ℃ ventilation environment for air drying, mixing the air-dried cake with a sodium hydroxide solution according to the mass-to-volume ratio of 20:1, and extracting protein at the pH of 9.0 and 55 ℃. And (3) carrying out spray drying on the extracted protein at 40 ℃ to obtain a dry protein product, uniformly mixing the dry protein product with water according to a proportion of 30%, then adding serine protease with the enzyme activity of 4000U/g, carrying out enzymolysis reaction at the pH of 10 and at the temperature of 45 ℃, and carrying out spray drying on an enzymolysis product to obtain the walnut selenium polypeptide dry powder.
B) And carrying out superfine grinding on the selenium-rich dendrobium leaves, and sieving with a 400-mesh sieve to obtain the selenium-rich dendrobium superfine powder.
3. And mixing the walnut selenium polypeptide dry powder with the selenium-rich dendrobe superfine powder to obtain the selenium-rich walnut dendrobe composition.
Example 2
Fully mixing 25 parts of walnut selenium polypeptide dry powder in example 1, 15 parts of selenium-rich dendrobe superfine powder in example 1, 2 parts of stevioside, 0.2 part of magnesium stearate, 20 parts of maltodextrin and 30 parts of pure water, granulating, drying at 50 ℃ until the moisture content is lower than 0.5%, sieving with a 100-mesh sieve, tabletting and demolding to obtain the selenium-rich walnut dendrobe tablet.
Example 3
Fully mixing 35 parts of walnut selenium polypeptide dry powder in example 1, 25 parts of selenium-rich dendrobe superfine powder in example 1, 5 parts of stevioside, 0.5 part of magnesium stearate, 30 parts of maltodextrin and 40 parts of pure water, granulating, drying at 50 ℃ until the moisture content is lower than 0.5%, sieving with a 100-mesh sieve, tabletting and demolding to obtain the selenium-rich walnut dendrobe tablet.
Example 4
Fully mixing 30 parts of walnut selenium polypeptide dry powder in example 1, 20 parts of selenium-rich dendrobe superfine powder in example 1, 4 parts of stevioside, 0.4 part of magnesium stearate, 25 parts of maltodextrin and 35 parts of pure water, granulating, drying at 50 ℃ until the moisture content is lower than 0.5%, sieving with a 100-mesh sieve, tabletting and demolding to obtain the selenium-rich walnut dendrobe tablet.
Example 5
Antioxidant activity determination of walnut selenium polypeptide
1) DPPH free radical scavenging:
2mg, 4mg, 6mg, 8mg and 10mg of the walnut selenium polypeptide dry powder described in example 1 are respectively dissolved in 10mL of purified water to prepare sample solutions of 0.2mg/mL, 0.4mg/mL, 0.6mg/mL, 0.8mg/mL and 1 mg/mL. Putting 4mL of sample solution with different concentrations in a test tube, adding 4mL of DPPH solution (0.1mmol L, prepared by using 95% ethanol as solvent), mixing, reacting at room temperature in dark for 30min, adjusting to zero with distilled water, and making into the final productObtaining a sample group, and measuring the light absorption value A at the wavelength of 517nm0. Replacing DPPH solution with 95% ethanol solution to obtain control group 1, and measuring its absorbance A at wavelength of 517nm1. Replacing sample liquid with distilled water under the same conditions to obtain control group 2, and measuring its light absorption value A at wavelength of 517nm2. According to the formula DPPH & clearance (%) - (1- (A)0-A1/A2) The DPPH radical clearance was calculated by x 100 and the results are shown in Table 1 below.
TABLE 1 clearance of DPPH & free radical by selenium-enriched walnut polypeptides of different concentrations
| Selenium-rich walnut polypeptide concentration (mg/mL) | DPPH. clearance% |
| 0.2 | 35.08 |
| 0.4 | 40.13 |
| 0.6 | 47.25 |
| 0.8 | 58.42 |
| 1 | 70.44 |
2) OH radical scavenging:
respectively taking 2mg, 4mg, 6mg, 8mg and 10mg of the selenium of the walnut in the embodiment 1The polypeptide dry powder was dissolved in 10mL of purified water to prepare 0.2mg/mL, 0.4mg/mL, 0.6mg/mL, 0.8mg/mL, and 1mg/mL of sample solutions. Sequentially adding 1mL of 1, 10-phenanthroline (1.865mmol/L) and 2mL of sample solutions with different concentrations into a test tube, uniformly mixing, and then adding 1mL of LFeSO4·7H2O (1.865mmol/L), shaking and mixing, and finally adding 1mLH2O2The solution (0.03%, v/v) was reacted in a 37 ℃ water bath for 60min, and the absorbance A was measured at 536nm while using distilled water instead of the sample solution as a negative control AjBlank control A using distilled water instead of hydrogen peroxide0. The ability of the sample solution to scavenge hydroxyl radicals was expressed by the formula OH radical scavenging ratio (%) ((A-A)0)/(A0-Aj) X 100). In the formula: a is the absorbance of the sample solution; a. thejAbsorbance of negative control; a. the0Blank absorbance. The calculation results are shown in table 2 below.
TABLE 2 clearance of selenium-enriched walnut polypeptides at different concentrations for OH free radical
| Selenium-rich walnut polypeptide concentration (mg/mL) | OH clearance% |
| 0.2 | 33.21 |
| 0.4 | 35.65 |
| 0.6 | 44.87 |
| 0.8 | 47.37 |
| 1 | 49.55 |
As can be seen, the selenium-rich walnut polypeptide solutions with different concentrations have obvious effect of removing DPPH & OH, and the removal rate is increased along with the increase of the mass concentration of the polypeptide. DPPH & and & OH clearance increases with increasing polypeptide selenium content at the same sample concentration. Therefore, the walnut selenium polypeptide has good antioxidant activity, and selenium enrichment can increase the antioxidant activity of the walnut polypeptide.
2. Determination of antioxidant activity of selenium-rich dendrobium superfine powder
The selenium-rich dendrobium nobile ultrafine powder prepared in the embodiment 1 is mixed and leached with hot water at the temperature of 90 ℃ in a volume ratio of 1:2, after leaching is carried out for 3 hours, the leaching solution is concentrated to 1/10 volumes, then ethanol with the volume fraction of 95% being 6 times of the volume is used for carrying out precipitation for 3 times, and the obtained precipitation is dried at the temperature of 55 ℃. Dissolving the 2mg, 4mg, 6mg, 8mg and 10mg of the precipitates in 10mL of pure water respectively to obtain dendrobium officinale polysaccharide solutions with different mass concentrations.
1) Elimination of DPPH · free radicals:
adding 0.5mL of dendrobium officinale polysaccharide solutions with different mass concentrations and 2.5mL of DPPH solution with volume fraction of 0.004% into the sample tube; the control tube uses volume fraction 95% ethanol instead of DPPH.solution; the blank tube uses distilled water to replace the dendrobium officinale polysaccharide solution. The 3 groups of test tubes are placed in a water bath at 30 ℃ and are subjected to light-proof reaction for 30min, then 0.5mL of distilled water and 2.5mL of 95% ethanol are used for zero setting, the absorbance A is respectively measured, and the test is repeated for three times to obtain the average value. Clearance rate according to the formula (A)Blank space-(ASample (I)-AControl))/ABlank spaceThe calculated DPPH-clearance rate of the dendrobium officinale polysaccharide is shown in the following table 3.
TABLE 3 clearance of DPPH free radical of selenium-rich dendrobe polysaccharide solutions of different concentrations
| Selenium-rich dendrobium polysaccharide solution mass concentration (mg/mL) | DPPH. clearance% |
| 0.2 | 21.33 |
| 0.4 | 36.29 |
| 0.6 | 38.24 |
| 0.8 | 41.33 |
| 1 | 44.70 |
2) Removal of. OH free radical:
1mL of 0.15mmol/L FeSO is added into a sample tube40.4mL of 2mmol/L salicylic acid, 0.2mL of dendrobium officinale polysaccharide solutions with different mass concentrations, and 1mL of 6mmol/L H2O2And 0.4mL of distilled water; the blank group uses distilled water to replace the dendrobium officinale polysaccharide solution, and the control group uses distilled water to replace salicylic acid. And (3) placing the 3 groups of test tubes into a water bath kettle at 37 ℃, taking out and cooling after 1 hour, adjusting to zero by using distilled water, respectively measuring absorbance A, setting 3 times of experiments, and taking an average value. The smaller the absorbance, the better the effect of eliminating. OH. Clearance rate according to formula (A)Blank space-(ASample (I)-AControl))/ABlank spaceThe removal rate of dendrobium officinale polysaccharide on OH obtained by calculating the multiplied by 100% is shown in the following table 4.
TABLE 4 clearance of OH free radical by Se-enriched dendrobe polysaccharide solution with different concentration
| Selenium-rich dendrobium polysaccharide solution mass concentration (mg/mL) | OH clearance% |
| 0.2 | 30.15 |
| 0.4 | 36.52 |
| 0.6 | 47.11 |
| 0.8 | 51.43 |
| 1 | 62.25 |
3. Determination of antioxidant activity of selenium-rich walnut-dendrobium tablet
1) DPPH free radical scavenging:
2mg, 4mg, 6mg, 8mg and 10mg of the selenium-rich walnut dendrobium tablet described in the embodiment 3 are respectively dissolved in 10mL of purified water to prepare sample solutions of 0.2mg/mL, 0.4mg/mL, 0.6mg/mL, 0.8mg/mL and 1 mg/mL. Putting 4mL of sample solution with different concentrations in a test tube, adding 4mL of DPPH solution (0.1mmol/L, prepared by using 95% ethanol as solvent), mixing, reacting at room temperature in dark for 30min, adjusting to zero with distilled water to obtain a sample group, and measuring the light absorption value A at a wavelength of 517nm0. The other conditions were not changed, and 95% ethanol solution was used instead of DPPH solution to prepare control group 1, and its absorbance A was measured at a wavelength of 517nm1. The other conditions are not changed, and the other conditions are not changed,using distilled water instead of sample solution to prepare control group 2, and measuring its light absorption value A at wavelength of 517nm2. According to the formula DPPH & clearance (%) - (1- (A)0-A1/A2) The DPPH.radical scavenging ratio calculated by X100 is shown in Table 5 below.
TABLE 5 clearance of DPPH free radical of selenium-enriched walnut and dendrobium tablets with different concentrations
| Selenium-rich walnut dendrobium tablet concentration (mg/mL) | DPPH. clearance% |
| 0.2 | 38.23 |
| 0.4 | 46.78 |
| 0.6 | 54.32 |
| 0.8 | 62.64 |
| 1 | 73.55 |
2) OH radical scavenging:
2mg, 4mg, 6mg, 8mg and 10mg of walnut selenium polypeptide dry powder are respectively dissolved in 10mL of purified water to prepare sample solutions of 0.2mg/mL, 0.4mg/mL, 0.6mg/mL, 0.8mg/mL and 1 mg/mL. Sequentially adding 1mL of 1, 10-phenanthroline (1.865mmol/L) and 2mL of sample liquid with different concentrations into a test tube, uniformly mixing, and then adding 1mL of LFeSO4·7H2O (1.865mmol/L), shaking and mixing, and finally adding 1mLH2O2The solution (0.03%, v/v) was reacted in a 37 ℃ water bath for 60min, and the absorbance A was measured at 536nm while using distilled water instead of the sample solution as a negative control AjBlank control A using distilled water instead of hydrogen peroxide0. The ability of the sample solution to scavenge hydroxyl radicals was expressed by the formula OH radical scavenging ratio (%) ((A-A)0)/(A0-Aj) X 100 is calculated as shown in table 6 below. In the formula: a is the absorbance of the sample solution; a. thejAbsorbance of negative control; a. the0Blank absorbance.
TABLE 6 clearance of OH free radical by selenium-enriched walnut and dendrobium tablets with different concentrations
| Selenium-rich walnut dendrobium tablet concentration (mg/mL) | OH clearance% |
| 0.2 | 45.53 |
| 0.4 | 48.28 |
| 0.6 | 52.14 |
| 0.8 | 64.73 |
| 1 | 71.16 |
According to the embodiment, the walnut selenium polypeptide and the selenium-rich dendrobium powder are compounded by taking the peach selenium polypeptide dry powder and the selenium-rich dendrobium superfine powder as raw materials, so that the oxidation resistance is obviously improved. According to the selenium-enriched walnut dendrobium tablet, the organic selenium content in the product is effectively improved through control over walnuts and dendrobium, the production process adopts the technology of cold pressing and low-temperature extraction, the denaturation of organic selenium under the high-temperature processing condition is prevented, the selenium content of the prepared selenium-enriched walnut dendrobium tablet is improved, the selenium polypeptide activity is higher, the selenium-enriched walnut dendrobium tablet is more beneficial to human body absorption, and the selenium-enriched walnut dendrobium tablet has good antioxidant and anti-aging effects.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present specification, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (10)
1. The selenium-rich walnut and dendrobium composition is characterized by comprising 25-35 parts by weight of walnut selenium polypeptide dry powder and 15-25 parts by weight of selenium-rich dendrobium superfine powder.
2. The selenium-enriched walnut dendrobium composition of claim 1, wherein the preparation of the walnut selenium polypeptide dry powder comprises the following steps:
1) squeezing selenium-rich walnut to obtain walnut oil and cake;
2) mixing the cake with ether to extract lipid to obtain defatted walnut cake;
3) air-drying the degreased walnut cake, and mixing with alkali liquor to extract protein;
4) mixing the extracted protein with serine protease for enzymolysis reaction, and drying the enzymolysis product to obtain the walnut selenium polypeptide dry powder.
3. The selenium-rich walnut dendrobium composition of claim 2, wherein the mass ratio of the cake meal to the diethyl ether in the step 2) is 2.5: 1-1.5: 1.
4. the selenium-rich walnut-dendrobium composition of claim 2, wherein the mass-to-volume ratio of the defatted walnut cake meal to the alkali liquor in the step 3) is 18: 1-23: 1, the temperature for extracting the protein is 50-60 ℃, and the pH value is 8.0-9.5.
5. The selenium-rich walnut dendrobium composition of claim 2, wherein the protein extracted in step 3) is dried and then uniformly mixed with water, and the mass percentage of the protein in the water is 25-35%.
6. The selenium-rich walnut dendrobium composition according to claim 2, wherein the enzyme activity of serine protease in the step 4) is more than or equal to 4000U/g.
7. The selenium-rich walnut-dendrobium composition of claim 1, wherein the selenium content of the walnut selenium polypeptide dry powder is more than or equal to 1.5mg/kg, and the selenium content of the selenium-rich dendrobium superfine powder is more than or equal to 2 mg/kg.
8. The selenium-rich walnut dendrobium composition of claim 7, wherein the particle size of the selenium-rich dendrobium superfine powder is less than or equal to 38 μm.
9. The selenium-rich walnut dendrobium tablet is characterized by comprising the following raw materials: 25-35 parts of walnut selenium polypeptide dry powder, 15-25 parts of selenium-enriched dendrobe superfine powder, 2-5 parts of stevioside, 0.2-0.5 part of magnesium stearate, 20-30 parts of maltodextrin and 30-40 parts of pure water.
10. The use of the selenium-rich walnut dendrobe composition of any one of claims 1 to 8 or the walnut dendrobe tablet of claim 9 in the preparation of an antioxidant drug or health product.
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