CN108478466B - Ceramide for moisturizing skin and preparation method thereof - Google Patents
Ceramide for moisturizing skin and preparation method thereof Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/68—Sphingolipids, e.g. ceramides, cerebrosides, gangliosides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/733—Alginic acid; Salts thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/04—Antipruritics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
Abstract
The invention discloses ceramide for skin moisturizing and a preparation method thereof. The ceramide for moisturizing skin disclosed by the invention is rich in konjak ceramide, rice ceramide and rice bran ceramide, has good water solubility, strong stability, easiness in absorption and quick response, and has the functions of whitening, moisturizing, reducing water loss, resisting oxidation, improving rough skin and pruritus, maintaining skin barrier and the like.
Description
Technical Field
The invention relates to the technical field of ceramide for moisturizing skin.
Background
Ceramide (Ceramide), a class of compounds formed by combining sphingosine and fatty acid through amide bonds, has the functions of whitening, moisturizing, resisting aging, maintaining skin barriers and the like. Ceramide is the main component of intercellular lipid of stratum corneum, about 40% -60% of the total amount, and its excellent hydrophilic property and unique arrangement mode can effectively prevent water loss. The ceramide content in the horny layer decreases with age, and atopic dermatitis and the like may cause the ceramide content in the horny layer to decrease. Grubauer et al reported that the skin of a male hairless mouse aged 1-3 months was treated with an organic solvent such as acetone to study the relationship between skin barrier function and skin lipid, and the results showed that the skin barrier function decreased with the decrease in skin lipid content. Holleran and the like study the relation between the epidermal barrier function and the synthesis of sphingolipids by adopting an infiltration mode, and the result shows that when the skin of hairless mice is treated by acetone to cause skin barrier dysfunction, the synthesis of the sphingolipids is increased, after the acetone treatment is carried out for 5-7 h, the synthesis of the sphingolipids can reach 170%, and after 24h, the skin barrier function is recovered to be normal, and the synthesis of the sphingolipids is also recovered to be normal. It follows that ceramides play a very important role in maintaining the skin barrier function. It is found that the high purity plant-derived ceramide refined by unique extraction process is more easily absorbed than general diet, sphingosine decomposed from plant-derived ceramide can promote synthesis of ceramide and activation of enzyme in epidermis, sphingosine can promote proliferation of fibroblast and production of collagen, and the content of ceramide in epidermis is increased by taking plant-derived ceramide. The moisture content of the skin is about 18% -20% of the body weight, the moistening and elasticity of the skin are closely related to the moisture contained in the epidermis, and excessive loss of the skin moisture can cause the skin to be dry and even chapped. Imokawa and the like adopt organic solvent treatment to remove stratum corneum lipid, and find that the skin is continuously cracked (>4 days), scales appear, and the skin conductivity is obviously reduced. The extracted lipid components of the horny layer of the epidermis are respectively subjected to local skin tests under the same conditions, and the effects of the lipid components on the skin moisture recovery are discussed, so that the results show that the skin conductivity can be obviously improved by the local application of ceramide, and the effects of free fatty acid and cholesterol ester are not obvious. Ceramides have a strong ability to associate with water molecules, and maintain skin moisture by forming a network structure in the stratum corneum, thereby maintaining skin moisture. During the aging process of the skin, lipid synthesis is reduced and the ceramide content in the stratum corneum is reduced. Skin aging is characterized by dry, desquamation, roughness, loss of luster, thinning of the stratum corneum, increased wrinkles and decreased elasticity. The ceramide can increase ceramide content in horny layer of epidermis, improve dry, desquamation and pachylosis of skin, increase horny layer thickness of epidermis, increase water holding capacity of skin, reduce wrinkle, enhance skin elasticity, and delay skin aging.
Disclosure of Invention
The invention provides ceramide for moisturizing skin and a preparation method thereof. The invention adopts the following technical scheme:
the ceramide for moisturizing the skin is prepared from the following raw materials: konjak ceramide, rice ceramide, and rice bran ceramide.
The ceramide for moisturizing the skin is prepared from the following raw materials: konjak ceramide, rice bran ceramide, erythritol, sodium alginate, xanthan gum and soybean protein powder.
The ceramide for moisturizing skin is prepared from the following raw materials in parts by weight: 3-8 parts of konjak ceramide, 12-16 parts of rice ceramide, 10-15 parts of rice bran ceramide, 0.05-0.2 part of absorption promoter, 8-12 parts of erythritol, 1-5 parts of sodium alginate, 0.2-0.8 part of xanthan gum and 800 parts of soybean protein powder.
The ceramide for moisturizing skin is prepared from the following raw materials in parts by weight: 3-8 parts of konjak ceramide, 12-16 parts of rice ceramide, 10-15 parts of rice bran ceramide, 0.05-0.2 part of absorption promoter, 3-6 parts of drone pupa extract, 8-12 parts of erythritol, 1-5 parts of sodium alginate, 0.2-0.8 part of xanthan gum and 500-800 parts of soybean protein powder.
The ceramide for moisturizing skin is prepared from the following raw materials in parts by weight: 3-8 parts of konjak ceramide, 12-16 parts of rice ceramide, 10-15 parts of rice bran ceramide, 0.05-0.2 part of absorption promoter, 3-6 parts of drone pupa extract, 8-12 parts of erythritol, 1-5 parts of sodium alginate, 0.2-0.8 part of xanthan gum, 500-800 parts of soybean protein powder and 0.02-0.1 part of preservative.
According to any one of the technical schemes, the absorption enhancer is malic acid and fenugreek extract according to the mass ratio of 1: (8-10) the method for preparing the fenugreek extract, comprising: respectively crushing the fenugreek seeds and the fenugreek leaves, sieving the crushed fenugreek seeds and the crushed fenugreek leaves by a sieve of 10 meshes, mixing the crushed fenugreek seeds and the crushed fenugreek leaves according to the mass ratio of 1 (5-7) to obtain a mixture, adding 30-50g of the mixture into 220g of water with 180 DEG, preserving the heat for 2-4 hours at 40-50 ℃, heating to 55-65 ℃, stirring for 2-4 hours at 55-65 ℃ and the rotation speed of 100 DEG for 300 r/min, sieving by a sieve of 300 DEG, evaporating and concentrating the filtrate to an extract with the relative density of 1.1-1.2 measured at 50 ℃, and drying the extract for 4-8 hours under the conditions of the absolute pressure of 0.03-0.05MPa and the temperature of 40-50 ℃ to obtain the fenug. The mixture of malic acid and the fenugreek extract is found to improve the water content of the skin, so that the ceramide can be well absorbed and utilized by the human body, and the skin moisturizing effect is improved.
According to any one of the technical scheme, the preparation method of the drone pupa extract comprises the following steps: 20-30g of drone pupa freeze-dried powder and water according to the material-liquid ratio of 1: (5-8) mixing the mixture according to the g/ml ratio, stirring the mixture for 20-50 minutes at the rotating speed of 300 revolutions per minute of 100-; carrying out rotary evaporation concentration on the primary filtrate at the temperature of 35-45 ℃, the rotation speed of 80-150 r/min and the absolute pressure of 0.04-0.07MPa to 1/5-1/4 of the original volume, and drying the concentrated solution at the absolute pressure of 0.01-0.04MPa and the temperature of 35-45 ℃ for 1-4 hours to obtain an extract I; mixing the primary filter cake with 0.02-0.06g of papain and 300g of 100-plus-energy water, carrying out enzymolysis for 24-48 hours at 35-45 ℃, heating to 85-95 ℃, carrying out heat preservation for 15-30 minutes at 85-95 ℃, and sieving with a 200-plus-energy 300-mesh sieve to obtain a secondary filter cake and a secondary filtrate; and (3) performing rotary evaporation concentration on the secondary filtrate at the temperature of 35-45 ℃, the rotation speed of 80-150 r/min and the absolute pressure of 0.04-0.07MPa to 1/4-1/3 of the original volume, drying the concentrated solution at the absolute pressure of 0.01-0.05MPa and the temperature of 35-50 ℃ for 3-6 hours to obtain an extract II, and uniformly mixing the extract I and the extract II according to the mass ratio of (1-3) to obtain the drone pupa extract. Further preferably, the drone pupa extract is obtained by uniformly mixing the extract I and the extract II according to the mass ratio of 3: 1. The drone pupa is an ideal nutritional food containing high protein, low fat, multiple vitamins and trace elements, has the effects of delaying senility, enhancing immunity, enabling skin to be glossy and hair to be black and bright, and has good effects of maintaining beauty and keeping young.
According to any one of the technical schemes, the preservative is an agaricus bisporus extract and/or a gorgon fruit extract, the preservative is further preferably a mixture of the agaricus bisporus extract and the gorgon fruit extract, and the mass ratio of the agaricus bisporus extract to the gorgon fruit extract is (1-3): (1-3).
The invention also provides a preparation method of the ceramide for moisturizing skin, which comprises the following steps: weighing the components according to the parts by weight, and stirring the components at the temperature of between 25 and 30 ℃ and the rotating speed of 100-300 r/min for 40 to 120 minutes to obtain the ceramide for moisturizing the skin.
The invention has the beneficial effects that:
the ceramide for moisturizing skin disclosed by the invention is rich in konjak ceramide, rice ceramide and rice bran ceramide, has good water solubility, strong stability, easiness in absorption and quick response, and has the functions of whitening, moisturizing, reducing water loss, resisting oxidation, improving rough skin and pruritus, maintaining skin barrier and the like.
On the basis of the common knowledge in the field, the above preferred conditions can be combined randomly to obtain the preferred embodiments of the invention.
Detailed Description
The main raw materials and instruments used in the examples were as follows:
konjak ceramide is commercially available. In the embodiment of the invention, the konjak ceramide is prepared according to patent application example 1 of CN 103755583.
Rice ceramide, commercially available. The preparation method of the rice ceramide in the embodiment of the invention comprises the following steps: pulverizing 20 weight parts of rice, sieving with 50 mesh sieve, adding 80 weight parts of 95 vol% ethanol water solution, stirring at 40 deg.C and 200 rpm for 3 hr, sieving with 200 mesh sieve, evaporating and concentrating the filtrate to obtain extract with relative density of 1.12 at 50 deg.C, and performing CO extraction on the extract2Supercritical fluid extraction at 38 deg.C under 15MPa for 1 hr with CO2The flow rate is 10L/h, and the obtained supercritical CO containing the extract2The fluid is decompressed, analyzed and separated to obtain an extraction product, the separation temperature is 35 ℃, the separation pressure is 5Mpa,collecting the obtained extraction product, namely the rice ceramide.
The rice bran ceramide is extracted from rice bran, and the ceramide content is 10%. West Anwan breed science and technology, Inc., Cat number WZ-XA 02.
Erythritol, CAS No.: 149-32-6.
Sodium alginate, viscosity (20 ℃): 800-1000 mPas, execution Standard GB1886.243-2016, Haematococcus qingdao, Inc.
Xanthan gum, CAS No.: 11138-66-2. Supplied by Shandong Fufeng fermentation Co.
Soy protein powder, auspicious food science and technology ltd, anyang, implements standard GB 20371-2016.
Malic acid, CAS No.: 6915-15-7.
Fenugreek seeds and fenugreek leaves, fresh mature seeds and fresh leaves of fenugreek (Latin scientific name: Trigonella foenum-graecum L.) were used. The fenugreek seeds are used as medicines and can tonify kidney, strengthen yang, eliminate phlegm and remove dampness; the tender stem and leaf can be used as vegetable; the stems, leaves or seeds are dried in the sun, ground into powder, added into flour and steamed as a flavoring agent. The Trigonella foenum-graecum seed also has effects of reducing blood sugar, reducing blood lipid, resisting gastric ulcer, resisting tumor, and treating chronic renal failure.
Drone pupa, which is unfertilized egg of queen bee in the drone, is hatched into larva, and the larva enters a pupa stage after 7 days of growth and development, wherein the body of the drone pupa is the drone pupa. The drone pupa is an ideal nutritional food containing high protein, low fat, multiple vitamins and trace elements, has the effects of delaying senility, enhancing immunity, enabling skin to be glossy and hair to be black and bright, and has good effects of maintaining beauty and keeping young. The drone pupa freeze-dried powder can be purchased from the market, and can also be prepared by carrying out low-temperature freeze drying and crushing on fresh drone pupas from which impurities are removed. The drone pupa freeze-dried powder used in the embodiment of the invention is provided by Hangzhou Baoling Yangtai Hei biotechnology limited, and the product number is as follows: BD001 LP.
Papain, CAS No. 9001-73-4, enzyme activity: 2000u/mg, Shanghai-sourced biosciences, Inc.
Agaricus bisporus extract, the scientific name of agaricus bisporus latin: agaricus bisporus, variety: AS 2796, origin: shandong Cui county. The preparation method of the agaricus bisporus extract in the embodiment of the invention comprises the following steps: crushing agaricus bisporus, sieving the agaricus bisporus by a 10-mesh sieve, mixing 50g of the crushed agaricus bisporus with 65% ethanol water solution according to the material-liquid ratio of 1:5g/mL, homogenizing for 10 minutes at the rotating speed of 12000 r/min to obtain slurry, heating the slurry to 75 ℃, stirring for 5 hours at the conditions of 75 ℃ and the rotating speed of 200 r/min, sieving by a 200-mesh sieve, evaporating and concentrating the filtrate to obtain an extract with the relative density of 1.13 determined at 50 ℃, and drying the extract for 8 hours under the conditions of 45 ℃ and the absolute pressure of 0.06MPa to obtain the agaricus bisporus extract.
Semen euryales extract, semen euryales is fresh mature kernel of Euryale ferox Salisb, a plant of Nymphaeaceae, and is produced in Heilongjiang. The preparation method of the gorgon fruit extract in the embodiment of the invention comprises the following steps: crushing gordon euryale seeds, sieving the crushed gordon euryale seeds with a 10-mesh sieve, mixing the crushed gordon euryale seeds with 45% ethanol water according to a material-liquid ratio of 1:5g/mL, stirring the mixture at 30 ℃ for 40 minutes at a rotating speed of 200 revolutions per minute, then performing microwave extraction, wherein the microwave frequency is 2450MHz, the microwave power is 500W, the microwave extraction time is 40 minutes, sieving the mixture with a 200-mesh sieve after the microwave extraction, evaporating and concentrating the filtrate to obtain an extract with the relative density of 1.12 measured at 50 ℃, and drying the extract for 10 hours under the condition of 50 ℃ and the absolute pressure of 0.04MPa to obtain the gordon euryale seed extract.
The invention is further illustrated by the following examples:
example 1
The ceramide for moisturizing skin is prepared from the following raw materials in parts by weight: 6 parts of konjak ceramide, 15 parts of rice ceramide and 12 parts of rice bran ceramide.
The preparation method of ceramide for moisturizing skin comprises the following steps: weighing the components in parts by weight, and stirring at 25 ℃ and a rotating speed of 200 r/min for 40 minutes to obtain the ceramide for moisturizing skin.
The ceramide for moisturizing skin disclosed by the invention is rich in konjak ceramide, rice ceramide and rice bran ceramide, has good water solubility, strong stability, easiness in absorption and quick response, and has the functions of whitening, moisturizing, reducing water loss, resisting oxidation, improving rough skin and pruritus, maintaining skin barrier and the like.
Example 2
The ceramide for moisturizing skin is prepared from the following raw materials in parts by weight: 6 parts of konjak ceramide, 15 parts of rice ceramide, 12 parts of rice bran ceramide, 10 parts of erythritol, 3 parts of sodium alginate, 0.5 part of xanthan gum and 750 parts of soybean protein powder.
The preparation method of ceramide for moisturizing skin comprises the following steps: weighing the components in parts by weight, and stirring at 25 ℃ and a rotating speed of 200 r/min for 40 minutes to obtain the ceramide for moisturizing skin.
Example 3
The ceramide for moisturizing skin is prepared from the following raw materials in parts by weight: 6 parts of konjak ceramide, 15 parts of rice ceramide, 12 parts of rice bran ceramide, 0.12 part of absorption enhancer, 10 parts of erythritol, 3 parts of sodium alginate, 0.5 part of xanthan gum and 750 parts of soybean protein powder.
The absorption promoter is prepared from malic acid and fenugreek extract according to a mass ratio of 1: 10, in a solvent. The preparation method of the fenugreek extract comprises the following steps: respectively crushing the fenugreek seeds and the fenugreek leaves, sieving the crushed fenugreek seeds and the fenugreek leaves by a sieve of 10 meshes, mixing the crushed fenugreek seeds and the crushed fenugreek leaves according to the mass ratio of 1:5 to obtain a mixture, adding 35g of the mixture into 200g of water, preserving the heat at 45 ℃ for 3 hours, heating to 60 ℃, stirring at 60 ℃ and the rotation speed of 200 r/min for 2.5 hours, sieving by a sieve of 100 meshes, evaporating and concentrating the filtrate to an extract with the relative density of 1.12 determined at 50 ℃, and drying the extract for 6 hours at the absolute pressure of 0.04MPa and the temperature of 45 ℃ to obtain the fenug.
The preparation method of ceramide for moisturizing skin comprises the following steps: weighing the components in parts by weight, and stirring at 25 ℃ and a rotating speed of 200 r/min for 40 minutes to obtain the ceramide for moisturizing skin.
Example 4
The ceramide for moisturizing skin is prepared from the following raw materials in parts by weight: 6 parts of konjak ceramide, 15 parts of rice ceramide, 12 parts of rice bran ceramide, 0.12 part of absorption enhancer, 4.5 parts of drone pupa extract, 10 parts of erythritol, 3 parts of sodium alginate, 0.5 part of xanthan gum and 750 parts of soybean protein powder.
The absorption promoter is prepared from malic acid and fenugreek extract according to a mass ratio of 1: 10, in a solvent. The preparation method of the fenugreek extract is the same as that of example 3.
The preparation method of the drone pupa extract comprises the following steps: 25g of drone pupa freeze-dried powder and water are mixed according to a material-liquid ratio of 1: mixing the materials in a ratio of 7g/ml, stirring the mixture for 30 minutes at a rotating speed of 200 revolutions per minute to obtain slurry, mixing the slurry and acetone according to a mass ratio of 1:2, stirring at 25 deg.C and 50 rpm for 20 min, centrifuging at 8000 rpm for 20 min, drying the precipitate at 30 deg.C for 15 min, then adding 300g of ethanol water solution with the volume fraction of 50% for ultrasonic extraction, wherein the ultrasonic frequency is 40KHz, the ultrasonic power is 500W, the ultrasonic extraction time is 40 minutes, sieving the mixture by a 200-mesh sieve after the ultrasonic extraction to obtain a primary filter cake and a primary filtrate, carrying out rotary evaporation concentration on the primary filtrate at the temperature of 40 ℃, the rotating speed of 100 revolutions per minute and the absolute pressure of 0.06MPa to 1/5 of the original volume, and drying the concentrated solution at the absolute pressure of 0.02MPa and the temperature of 40 ℃ for 3 hours to obtain an extract I; mixing the primary filter cake with 0.05g of papain and 200g of water, carrying out enzymolysis for 36 hours at 40 ℃, heating to 90 ℃, carrying out heat preservation for 20 minutes at 90 ℃, sieving by a 200-mesh sieve to obtain a secondary filter cake and a secondary filtrate, carrying out rotary evaporation concentration on the secondary filtrate at 40 ℃, the rotation speed of 100 revolutions per minute and the absolute pressure of 0.06MPa to 1/4 of the original volume, drying the concentrated solution at the absolute pressure of 0.02MPa and the absolute pressure of 40 ℃ for 5 hours to obtain an extract II, and uniformly mixing the extract I and the extract II according to the mass ratio of 3:1 to obtain the drone pupa extract.
The preparation method of ceramide for moisturizing skin comprises the following steps: weighing the components in parts by weight, and stirring at 25 ℃ and a rotating speed of 200 r/min for 40 minutes to obtain the ceramide for moisturizing skin.
Example 5
Essentially the same as example 4, except that: in this example 5:
the preparation method of the drone pupa extract comprises the following steps: 25g of drone pupa freeze-dried powder and water are mixed according to a material-liquid ratio of 1: mixing the materials in a ratio of 7g/ml, stirring the mixture for 30 minutes at a rotating speed of 200 revolutions per minute to obtain slurry, mixing the slurry and acetone according to a mass ratio of 1:2, stirring at 25 deg.C and 50 rpm for 20 min, centrifuging at 8000 rpm for 20 min, drying the precipitate at 30 deg.C for 15 min, then adding 300g of ethanol water solution with the volume fraction of 50% for ultrasonic extraction, wherein the ultrasonic frequency is 40KHz, the ultrasonic power is 500W, the ultrasonic extraction time is 40 minutes, sieving the mixture by a 200-mesh sieve after the ultrasonic extraction to obtain a primary filter cake and a primary filtrate, carrying out rotary evaporation concentration on the primary filtrate at the temperature of 40 ℃, the rotating speed of 100 revolutions per minute and the absolute pressure of 0.06MPa to 1/5 of the original volume, and drying the concentrated solution at the absolute pressure of 0.02MPa and the temperature of 40 ℃ for 3 hours to obtain an extract I; mixing the primary filter cake with 0.05g of papain and 200g of water, carrying out enzymolysis for 36 hours at 40 ℃, heating to 90 ℃, carrying out heat preservation for 20 minutes at 90 ℃, sieving by a 200-mesh sieve to obtain a secondary filter cake and a secondary filtrate, carrying out rotary evaporation concentration on the secondary filtrate at 40 ℃, the rotation speed of 100 revolutions per minute and the absolute pressure of 0.06MPa to 1/4 of the original volume, drying the concentrated solution at the absolute pressure of 0.02MPa and the absolute pressure of 40 ℃ for 5 hours to obtain an extract II, and uniformly mixing the extract I and the extract II according to the mass ratio of 1:1 to obtain the drone pupa extract.
Example 6
Essentially the same as example 4, except that: in this example 6:
the preparation method of the drone pupa extract comprises the following steps: 25g of drone pupa freeze-dried powder and water are mixed according to a material-liquid ratio of 1: mixing the materials in a ratio of 7g/ml, stirring the mixture for 30 minutes at a rotating speed of 200 revolutions per minute to obtain slurry, mixing the slurry and acetone according to a mass ratio of 1:2, stirring at 25 deg.C and 50 rpm for 20 min, centrifuging at 8000 rpm for 20 min, drying the precipitate at 30 deg.C for 15 min, then adding 300g of ethanol water solution with the volume fraction of 50% for ultrasonic extraction, wherein the ultrasonic frequency is 40KHz, the ultrasonic power is 500W, the ultrasonic extraction time is 40 minutes, sieving the mixture by a 200-mesh sieve after the ultrasonic extraction to obtain a primary filter cake and a primary filtrate, carrying out rotary evaporation concentration on the primary filtrate at the temperature of 40 ℃, the rotating speed of 100 revolutions per minute and the absolute pressure of 0.06MPa to 1/5 of the original volume, and drying the concentrated solution at the absolute pressure of 0.02MPa and the temperature of 40 ℃ for 3 hours to obtain an extract I; mixing the primary filter cake with 0.05g of papain and 200g of water, carrying out enzymolysis for 36 hours at 40 ℃, heating to 90 ℃, carrying out heat preservation for 20 minutes at 90 ℃, sieving by a 200-mesh sieve to obtain a secondary filter cake and a secondary filtrate, carrying out rotary evaporation concentration on the secondary filtrate at 40 ℃, the rotation speed of 100 revolutions per minute and the absolute pressure of 0.06MPa to 1/4 of the original volume, drying the concentrated solution at the absolute pressure of 0.02MPa and the absolute pressure of 40 ℃ for 5 hours to obtain an extract II, and uniformly mixing the extract I and the extract II according to the mass ratio of 1:3 to obtain the drone pupa extract.
Example 7
The ceramide for moisturizing skin is prepared from the following raw materials in parts by weight: 6 parts of konjak ceramide, 15 parts of rice ceramide, 12 parts of rice bran ceramide, 0.12 part of absorption enhancer, 4.5 parts of drone pupa extract, 10 parts of erythritol, 3 parts of sodium alginate, 0.5 part of xanthan gum, 750 parts of soybean protein powder and 0.05 part of preservative.
The absorption promoter is prepared from malic acid and fenugreek extract according to a mass ratio of 1: 10, in a solvent. The preparation method of the fenugreek extract is the same as that of example 3. The preparation method of the drone pupae extract is the same as in example 4. The preservative is a mixture of an agaricus bisporus extract and a gorgon fruit extract, and the mass ratio of the agaricus bisporus extract to the gorgon fruit extract is 1: 1.
The preparation method of ceramide for moisturizing skin comprises the following steps: weighing the components in parts by weight, and stirring at 25 ℃ and a rotating speed of 200 r/min for 40 minutes to obtain the ceramide for moisturizing skin.
Example 8
Basically the same as example 7, except that in this example 8: the preservative is only an agaricus bisporus extract.
Example 9
Basically the same as example 7, except that in this example 9: the preservative is only a gordon euryale seed extract.
Test of moisturizing Effect
1. Test subject
1.1 inclusion criteria: the age of the voluntary subjects qualified by experience is between 30 and 50 years, and the skin moisture of the women is less than or equal to 12 percent. For a total of 180 persons.
1.2 exclusion criteria: pregnant or lactating women, allergic constitution and allergic to the product; patients with serious diseases of heart, liver, kidney and hematopoietic system, and endocrine diseases; psychotic patients; the judgment of the result is influenced by taking the medicine or health care product related to the tested function in a short time.
2. Experimental methods
The test group and the control group are randomly divided according to the skin moisture condition of the subject. And the balance test is carried out by considering the main factors influencing the result such as age and the like as much as possible. The experiment adopts a double-blind method, and the experiment is divided into groups: the groups were divided into 8 experimental groups and 1 control group, each group containing 20 persons. The water-based formulations of ceramide for moisturizing skin of examples 2 to 9 were administered to 8 test groups, respectively, once a day before sleep, and 100g of the water-based formulation of ceramide for moisturizing skin was administered each time. The control group took 100g of water daily before going to sleep. The experimental group and the control group were continuously administered for 30 days. The subjects discontinued the use of other oral and topical products related to skin moisture during the experiment. The original diet habit and normal diet were not changed during the experiment. The preparation method of the water flush agent of ceramide for moisturizing skin comprises the following steps: 1g of ceramide for skin moisturizing is mixed with 99g of water, and then stirred for 5 minutes at 30 ℃ at a rotating speed of 50 revolutions per minute, so that the water soluble granules of the ceramide for skin moisturizing are obtained.
3. Skin moisture test
In a wide and well ventilated examination room, the forehead of the subject was cleaned by dipping distilled water in a clean cotton ball under a quiet state, and the liquid was used 15 minutes after wiping
CM 825 measures the moisture content.
The skin moisturizing effect of the invention is tested by using ceramide, and the test result is shown in table 1.
Table 1: moisturizing effect test result table
| Skin moisture before consumption (%) | Skin moisture after eating (%) | |
| Control group | 8.45 | 8.47 |
| Example 2 | 8.41 | 10.62 |
| Example 3 | 8.47 | 12.09 |
| Example 4 | 8.46 | 14.96 |
| Example 5 | 8.49 | 13.82 |
| Example 6 | 8.43 | 13.27 |
| Example 7 | 8.44 | 18.65 |
| Example 8 | 8.42 | 16.29 |
| Example 9 | 8.45 | 15.58 |
The results of each example group compared with the control group, P <0.05, are statistically significant. The results between the various groups were statistically significant when compared to P' < 0.05.
In vivo antioxidant Activity detection
The experimental animal cleans 180 mice with half of each male and female, and the weight is 20 +/-0.5 g. Mice were bred at 25 ℃ with free drinking water, and after adaptive breeding for one week, the mice were randomly divided into 9 groups of 8 experimental groups and 1 control group, and 20 mice per group, 8 experimental groups were each individually gavaged with the skin moisturizing ceramides of examples 2-9, once a day, at a gavage amount of 0.003 g/mouse. The control group had a daily gavage of 0.003 g/mouse. The experimental group and the control group were continuously gavaged for 30 days. Fasted for 24h after the last gavage, blood was rapidly collected, red blood cells and serum were separated, and red blood cell superoxide dismutase (SOD) activity was detected using Hitachi 7170A full-automatic biochemical analyzer. The test results are shown in Table 2.
Table 2: in vivo antioxidant activity test result table
The results of the groups were compared, P < 0.05.
Therefore, the ceramide for moisturizing skin is rich in konjak ceramide, rice ceramide and rice bran ceramide, has good water solubility, strong stability, easy absorption and quick response, and has the functions of whitening, moisturizing, reducing water loss, resisting oxidation, improving rough skin and pruritus, maintaining skin barrier and the like. The ceramide for skin moisturizing can improve the SOD activity of cells, delay cell aging and maintain the skin tender and elastic.
The foregoing detailed description of the preferred embodiments of the invention has been presented. It should be understood that numerous modifications and variations could be devised by those skilled in the art in light of the present teachings without departing from the inventive concepts. Therefore, the technical solutions available to those skilled in the art through logic analysis, reasoning and limited experiments based on the prior art according to the concept of the present invention should be within the scope of protection defined by the claims.
Claims (3)
1. The ceramide for moisturizing skin is characterized by being prepared from the following raw materials in parts by weight: 3-8 parts of konjak ceramide, 12-16 parts of rice ceramide, 10-15 parts of rice bran ceramide, 0.05-0.2 part of absorption promoter, 3-6 parts of drone pupa extract, 8-12 parts of erythritol, 1-5 parts of sodium alginate, 0.2-0.8 part of xanthan gum, 500-800 parts of soybean protein powder and 0.02-0.1 part of preservative; the preservative is a mixture of an agaricus bisporus extract and a gorgon fruit extract, and the mass ratio of the agaricus bisporus extract to the gorgon fruit extract is 1: 1;
the absorption promoter is a mixture of malic acid and a fenugreek extract according to a mass ratio of 1 (8-10), and the preparation method of the fenugreek extract comprises the following steps: respectively crushing the fenugreek seeds and the fenugreek leaves, sieving the crushed fenugreek seeds and the crushed fenugreek leaves by a sieve of 10 meshes, mixing the crushed fenugreek seeds and the crushed fenugreek leaves according to the mass ratio of 1 (5-7) to obtain a mixture, adding 30-50g of the mixture into 220g of water with 180 DEG, preserving the heat for 2-4 hours at 40-50 ℃, heating to 55-65 ℃, stirring for 2-4 hours at 55-65 ℃ and the rotation speed of 100 DEG for 300 r/min, sieving by a sieve of 300 DEG, evaporating and concentrating the filtrate to an extract with the relative density of 1.1-1.2 measured at 50 ℃, and drying the extract for 4-8 hours under the conditions of the absolute pressure of 0.03-0.05MPa and the temperature of 40-50 ℃ to obtain the fenug.
2. The ceramide for moisturizing skin according to claim 1, wherein the drone pupa extract is prepared by the following method: 20-30g of drone pupa freeze-dried powder and water are mixed according to the material-liquid ratio of 1 g: (5-8) mixing the materials in a ml ratio, stirring the materials for 20-50 minutes at the rotating speed of 300-75 revolutions per minute to obtain slurry, mixing the slurry and acetone according to the mass ratio of 1 (1-3), stirring the materials for 10-30 minutes at the rotating speed of 25-75 revolutions per minute at the temperature of 25-30 ℃, centrifuging the materials for 10-30 minutes at the rotating speed of 5000-10000 revolutions per minute, drying the precipitates obtained by centrifuging the materials for 10-20 minutes at the temperature of 30-40 ℃, adding the precipitates into 250-350g of ethanol aqueous solution with the volume fraction of 40-60 percent for ultrasonic extraction, wherein the ultrasonic frequency is 20-40KHz, the ultrasonic power is 300-600W, the ultrasonic extraction time is 30-60 minutes, and passing through a 200-300-mesh sieve after the ultrasonic extraction to obtain a primary filter cake and primary filtrate; carrying out rotary evaporation concentration on the primary filtrate at the temperature of 35-45 ℃, the rotation speed of 80-150 r/min and the absolute pressure of 0.04-0.07MPa to 1/5-1/4 of the original volume, and drying the concentrated solution at the absolute pressure of 0.01-0.04MPa and the temperature of 35-45 ℃ for 1-4 hours to obtain an extract I; mixing the primary filter cake with 0.02-0.06g of papain and 300g of 100-plus-energy water, carrying out enzymolysis for 24-48 hours at 35-45 ℃, heating to 85-95 ℃, carrying out heat preservation for 15-30 minutes at 85-95 ℃, and sieving with a 200-plus-energy 300-mesh sieve to obtain a secondary filter cake and a secondary filtrate; and (3) performing rotary evaporation concentration on the secondary filtrate at the temperature of 35-45 ℃, the rotation speed of 80-150 r/min and the absolute pressure of 0.04-0.07MPa to 1/4-1/3 of the original volume, drying the concentrated solution at the absolute pressure of 0.01-0.05MPa and the temperature of 35-50 ℃ for 3-6 hours to obtain an extract II, and uniformly mixing the extract I and the extract II according to the mass ratio of (1-3) to obtain the drone pupa extract.
3. The method for preparing ceramide for skin moisture retention according to claim 1 or 2, wherein the ceramide for skin moisture retention is obtained by weighing the components in parts by weight and stirring at a rotation speed of 100-300 rpm for 40-120 minutes at 25-30 ℃.
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