CN102041280A - Method for extracting and comprehensively utilizing active matters of rice bran - Google Patents
Method for extracting and comprehensively utilizing active matters of rice bran Download PDFInfo
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- CN102041280A CN102041280A CN2009101533106A CN200910153310A CN102041280A CN 102041280 A CN102041280 A CN 102041280A CN 2009101533106 A CN2009101533106 A CN 2009101533106A CN 200910153310 A CN200910153310 A CN 200910153310A CN 102041280 A CN102041280 A CN 102041280A
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- rice bran
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- jidingsuan
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- ceramide
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Abstract
The invention discloses a method for comprehensively extracting active matters from rice processing byproducts such as rice bran and grain embryo. The rice bran, the grain embryo and the like are taken as raw materials, and the method comprises the following steps of: converting by using an endogenous enzyme method and extracting to obtain gamma-aminobutyric acid; extracting the active matters according to physical and chemical characteristics of the active matters in a certain order; extracting natural rice bran ceramide from a filter cake subjected to gamma-aminobutyric acid extraction by using an amphiprotic solvent; stepwise precipitating by using ethanol to obtain rice bran polysaccharide; and extruding and bulking the filter cake to prepare rice bran dietary fibers. The rice processing byproduct resources such as the rice bran and the grain embryo are fully utilized, the wastes generated in the production process are reduced, and higher economic and social benefits are achieved.
Description
Technical field
The present invention relates to actives extraction and method of comprehensive utilization in the rice processing byproducts such as rice bran, paddy embryo, be specifically related to isolation technique and comprehensively extract actives in rice bran, the paddy embryo etc., improve the added value of processing such as rice bran, paddy embryo, belong to rice intensive processing field.
Background technology
Data shows according to statistics, and Chinese annual paddy ultimate production accounts for 42% of national total output of grain about 2.0 hundred million t, account for about 35% of world's paddy ultimate production, but has more than 1,000 ten thousand tons rice bran not utilized by foodstuffs industry in the course of processing.Rice bran is rich in the active substance of physiological function brilliances such as nutrient substance such as quality protein, Mierocrystalline cellulose, mineral element, vitamin B group (VB1, VB2, VB6), a large amount of eatable celluloses and polysaccharide, 28 carbon alkanols, 30 carbon alkanols, ceramide.The fatty unsaturated fatty acids that is mainly in the rice bran, indispensable fatty acid content reaches 47%, also contains more than 70 kind of antioxidant component.Latest research proves that rice bran contains the nutritive ingredient and the 90% above needed by human body element of rice 64%.In the rice bran, protein content is about 12%～18%, fat 16%～20%, mineral substance 12%, food fibre are about 14%, carbohydrate total amount about 50%, these natural nutrient substances have multiple functions such as preventing cardiovascular disease, blood sugar regulation, prophylaxis of tumours, antifatigue, fat-reducing, beauty treatment, are described as " nutrition source of being bestowed by heaven " abroad.The U.S. and Japan research and develop the most flourishing country of rice bran resource at present in the world.
γ-An Jidingsuan (GABA) is a kind of nonprotein amino acid that extensively exists in the organism, produces through the L-Glutamic decarboxylase decarboxylation by L-glutamic acid or Sodium Glutamate, and be the main mediator of central nervous system.Have the brain of improvement function, adjust blood pressure, calm nerve, improve effects such as insomnia, renal function activation and liver function activation.Rice bran is the by product that paddy adds man-hour, because the rice embryo in the rice bran contains L-Glutamic decarboxylase and vigor is higher, glutamic acid rotating can be turned to GABA, so rice bran is the raw material of a kind of good preparation GABA.
Ceramide has skin and brightens, preserves moisture and alleviate effects such as allergic dermatitis symptom.It is raw material that original ceramide goods are mainly taken from wheat, and goods cost an arm and a leg.Now produce ceramide by rice bran, price is lower, the rice bran of containing ceramide massfraction 0.3% above powder-type and aqueous type goods is arranged at present in the Japanese market, as the important source material of beauty food.
Adopt different extraction processes can obtain multiple rice bran polysaccharide, as RBS, PBF-P, RBF-PM, MDP, RON, MGN-3 etc., they all have significant biological activity and nourishing function, as antitumor, immunostimulant and hypoglycemic etc.
Utilize in the rice bran abundant and comprehensively nutritive ingredient and food fibre exploitation nutrition and heath food, be study the earliest, rice bran food that product is maximum.As U.S. RiceX
TMRice bran nutrient fibre (the RiceX of company
TMFiber Compex), also there is similar products like in U.S. Li Pu Man.
In order to obtain the nutritive substance in rice bran, the paddy embryo etc., develop the domestic rice bran nutritive ingredient is prepared, the patent of rice bran aspect is a lot, introduced a kind of separation purification method of rice bran polysaccharide as " a kind of separation purification method of rice bran polysaccharide (ZL 200610166325.2) " of people such as Chen Zhenghang invention, " a kind of is the method (ZL 200610166426.X) of feedstock production high density γ-An Jidingsuan powder with the rice bran " of people such as Zhang Hui invention introduced and separated the method that obtains γ-An Jidingsuan etc. from rice bran.The invention provides the comprehensive preparation method of actives in a kind of rice bran, meet the green safety machine direction, the great development prospect is arranged.
Summary of the invention
The purpose of this invention is to provide a kind of difference rice bran actives preparation method in the past, utilize isolation technique in the γ-An Jidingsuan that extracts high added value, physics-chem characteristic according to other actives, obtain other actives of rice brans such as natural rice bran ceramide, rice bran polysaccharide and rice bran food fibre, make full use of Biological resources, reduce the generation of the waste pollution of production process, can produce higher economic benefit.
Isolation technique provided by the invention is comprehensively extracted the method for actives in the rice bran, be when extracting the actives γ-An Jidingsuan, utilize the filter cake after extracting, physics-chem characteristic according to actives, extract other actives in certain sequence, utilize amphoteric solvent to extract natural rice bran ceramide earlier, throw out adds water boil again and obtains rice bran polysaccharide, and remaining precipitation can get the rice bran food fibre.Step is as follows:
(1) endogenous enzymatic steps: with the by product rice bran in the polished rice course of processing, paddy embryo etc. is raw material, add entry, the weightmeasurement ratio of feed liquid is 1: 6～8, the glutamic acid compounds that adds rice bran weight 0.05%～0.5%, the soluble calcium salt that adds glutamic acid compounds weight 0.6%～1.0%, concentration according to 0.5～1.0mmol/L adds pyridoxal phosphate, regulates pH to 5.5～6.0, reacts 6～12h under 30～40 ℃ of conditions of temperature.
(2) γ-An Jidingsuan extraction step: supernatant liquor is got in the centrifugation of γ-An Jidingsuan pregnant solution, the ethanol that adds supernatant liquor volume 70%～80%, precipitation 10～14h, supernatant liquor is got in centrifugation, through behind the micro-filtration, filtrate through multiple-effect be evaporated to solid content reach 20%～30% after spraying drying, the air outlet temperature is 85～95 ℃, promptly gets the γ-An Jidingsuan powder.
(3) natural rice bran ceramide extraction step: the rice bran filter cake behind the extraction GABA adds amphoteric solvent, and the weight ratio of liquid material is 3～8: 1, amphoteric solvent methylene dichloride: methyl alcohol: the weight ratio of water is 10～30: 5～20: 0.5～3; Stir 3.5～7h at 35～60 ℃, filtered while hot is just told filtrate 1 and filter cake 1; Filtrate 1 is told throw out through the high-efficient silica gel column chromatography for separation-30～-15 ℃ of cooling precipitations, and use methylene dichloride: the weight ratio of methyl alcohol is 7～15: 0.5～1.5 mixed solvent wash-out, and solvent concentrates, and obtains the ceramide mixture; Filter cake 1 after natural rice bran ceramide extracts is standby.
(4) rice bran polysaccharide extraction step: the filter cake 1 in the step (3) is added water by the weight ratio 10～20: 0.5～3 of liquid material, boil 3～5h under the neutrallty condition, filtrate is separated with filter cake 2, and filtrate is obtained the rice bran polysaccharide crude product with 60%～80% ethanol sedimentation; Filter cake 2 after rice bran polysaccharide extracts is standby.
(5) rice bran food fibre extraction step: the filter cake 2 after step (4) rice bran polysaccharide extracted is through washing, centrifuge dehydration and oven dry, after be crushed to 160～200 orders again, the rice bran diet fiber product.
Feature of the present invention also is step (1), and the glutamic acid compounds of adding can be L-glutamic acid, Potassium glutamate, Sodium Glutamate etc.
Feature of the present invention also is step (1), and the soluble calcium salt of adding can be calcium acetate, calcium chloride etc.
Feature of the present invention also is step (2), supernatant liquor is through 0.4～10 μ m microfiltration membrane micro-filtration, again with inorganic ceramic film ultrafiltration under 0.2MPa pressure of 50nm, filtrate through multiple-effect be evaporated to solid content reach 20%～30% after spraying drying, the air outlet temperature is 85～95 ℃, promptly gets the high-content gamma-aminobutyric acid powder.
Feature of the present invention also is step (5), and filter cake 2 is pulverized the back extruding puffing through washing, centrifuge dehydration and oven dry, is crushed to 160～200 orders again, the rice bran diet fiber product.
Advantage of the present invention
1, according to the physics-chem characteristic of various activess, utilize different extraction means and different solvents, substep extracts, and is independent of each other, and extracts one by one.
2, at the rice bran actives that obtains several high added values with batch raw material simultaneously.
3, make full use of Biological resources, reduce the generation of the waste pollution of production process, produce higher economic benefit.
Description of drawings
Fig. 1, rice bran prepare rice bran actives process flow diagrams such as natural γ-An Jidingsuan, natural rice bran ceramide, rice bran polysaccharide and rice bran food fibre
Embodiment
Be described further below in conjunction with the extracting method of specific embodiment, to help understanding content of the present invention rice bran actives of the present invention.
Inventive embodiments 1
1. natural γ-An Jidingsuan transforms and extracts: fresh rice bran is pulverized the back and is crossed 36 mesh sieves, gets the rice bran 10kg after sieving, and adds 70kg water successively, 30g L-glutamic acid, 0.3g calcium chloride, add pyridoxal phosphate according to the concentration of 0.5mmol/L, under 40 ℃ of temperature, pH5.6 condition, react.Centrifugation gets supernatant liquor and filter cake 1 behind the 7h.Supernatant liquor adds 42L ethanol, precipitation 8h, the centrifuging and taking supernatant liquor, through behind the micro-filtration, inorganic ceramic film through 50nm carries out ultrafiltration under 0.2MPa pressure again, filtrate through two imitate be evaporated to solid content reach 30% after spraying drying, the air outlet temperature is 95 ℃, get high contain γ-An Jidingsuan powder 37g, ethanol recycling.
2. natural rice bran ceramide extracts: the filter cake 1 with step 1 adds 50Kg amphoteric solvent methylene chloride/water (20/10/1) at 40 ℃ of stirring 4h, filtered while hot, it is standby to obtain filter cake 2, filtrate is at-20 ℃ of cooling precipitation 12h, throw out dissolving back high-efficient silica gel column chromatography, eluent usefulness methylene chloride (9/1) obtains the yellow meal of 4.3g, contain 57% natural rice bran ceramide, solvent recuperation is utilized again.
3. rice bran polysaccharide extracts: the filter cake 2 with behind the step 2 extraction vegetative nerve acid amides, and added water than 10: 1 by the liquid material and transfer, boil 3h, filter, obtain filter cake 3, filtrate is used 60% ethanol sedimentation, and is centrifugal, obtains polysaccharide crude 572g, the ethanol recycling.
4. the rice bran food fibre extracts: filter cake 3 is pulverized the back extruding puffing through washing, centrifuge dehydration and oven dry, is crushed to 160 orders again, rice bran diet fiber product 3036g.
Claims (6)
1. method of from rice bran, paddy embryo, extracting gamma amino butyric acid, natural rice bran ceramide, rice bran polysaccharide, rice bran food fibre etc., it is characterized in that: described rice bran, paddy embryo are the by products in the polished rice course of processing, obtain γ-An Jidingsuan (GABA) through endogenous enzymatic conversion method, centrifugation obtains supernatant liquor, the gained supernatant liquor contains γ-An Jidingsuan, extract natural rice bran ceramide, rice bran polysaccharide more successively, last residuum can prepare the rice bran food fibre.
2. the actives of rice bran in certain sequence according to claim 1 extracts and the production method of comprehensive utilization, it is characterized in that the processing step of this method is as follows:
(1) step of described endogenous enzymatic conversion method is: with the by product rice bran in the polished rice course of processing, paddy embryo etc. is raw material, add entry, the weightmeasurement ratio of feed liquid is 1: 6～8, the glutamic acid compounds that adds rice bran weight 0.05%～0.5%, the soluble calcium salt that adds glutamic acid compounds weight 0.6%～1.0%, concentration according to 0.5～1.0mmol/L adds pyridoxal phosphate, regulates pH to 5.5～6.0, reacts 6～12h under 30～40 ℃ of conditions of temperature.
(2) purification process of described γ-An Jidingsuan is: supernatant liquor is got in the centrifugation of γ-An Jidingsuan pregnant solution, the ethanol that adds supernatant liquor volume 70%～80%, precipitation 10～14h, supernatant liquor is got in centrifugation, through behind the micro-filtration, filtrate through multiple-effect be evaporated to solid content reach 20%～30% after spraying drying, the air outlet temperature is 85～95 ℃, promptly gets the γ-An Jidingsuan powder.
(3) extracting method of described natural rice bran ceramide is: the rice bran filter cake behind the extraction GABA adds amphoteric solvent, and the weight ratio of liquid material is 3～8: 1, amphoteric solvent methylene dichloride: methyl alcohol: the weight ratio of water is 10～30: 5～20: 0.5～3; Stir 3.5～7h at 35～60 ℃, filtered while hot is just told filtrate 1 and filter cake 1; Filtrate 1 is told throw out through the high-efficient silica gel column chromatography for separation-30～-15 ℃ of cooling precipitations, and use methylene dichloride: the weight ratio of methyl alcohol is 7～15: 0.5～1.5 mixed solvent wash-out, and solvent concentrates, and obtains the ceramide mixture; Filter cake 1 after natural rice bran ceramide extracts is standby.
(4) extraction step of described rice bran polysaccharide is: with the weight ratio 10～20 of the filter cake 1 in the step (3) by the liquid material: 0.5～3 adds water, boil 3～5h under the neutrallty condition, filtrate is separated with filter cake 2, and filtrate is obtained the rice bran polysaccharide crude product with 60%～80% ethanol sedimentation; Filter cake 2 after rice bran polysaccharide extracts is standby.
(5) extraction step of described rice bran food fibre is: the filter cake 2 after step (4) rice bran polysaccharide is extracted is through washing, centrifuge dehydration and oven dry, after be crushed to 160～200 orders again, obtain outward appearance and be Powdered, color and luster and be the rice bran diet fiber product of micro white to white.
3. the method for transformation of γ-An Jidingsuan as claimed in claim 2, its feature also is step (1), the glutamic acid compounds of adding can be L-glutamic acid, Potassium glutamate, Sodium Glutamate etc.
4. the method for transformation of γ-An Jidingsuan as claimed in claim 2, its feature also is step (1), the soluble calcium salt of adding can be calcium acetate, calcium chloride etc.
5. the purification process of γ-An Jidingsuan as claimed in claim 2, its feature also is step (2), supernatant liquor is through 0.4～10 μ m microfiltration membrane micro-filtration, again with inorganic ceramic film ultrafiltration under 0.2MPa pressure of 50nm, filtrate through multiple-effect be evaporated to solid content reach 20%～30% after spraying drying, the air outlet temperature is 85～95 ℃, promptly gets the high-content gamma-aminobutyric acid powder.
6. rice bran food fibre preparation method as claimed in claim 2, its feature also is step (5), filter cake 2 is pulverized the back extruding puffing through washing, centrifuge dehydration and oven dry, is crushed to 160～200 orders again, the rice bran diet fiber product.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102225161A (en) * | 2011-05-12 | 2011-10-26 | 江南大学 | Method for preparing functional components of antioxidation, blood sugar reduction and cholesterol reduction by using rice bran |
| CN103193893A (en) * | 2013-04-18 | 2013-07-10 | 江南大学 | Preparation method of rice bran polysaccharide sulfate based on ionic liquid |
| CN104292349A (en) * | 2014-09-16 | 2015-01-21 | 沈阳师范大学 | Equipment and method for preparing carboxymethylated rice bran polysaccharide |
| CN104311437A (en) * | 2014-09-18 | 2015-01-28 | 苏州经贸职业技术学院 | Method for extracting, separating and purifying gamma-aminobutyric acid |
| CN104306391A (en) * | 2014-09-18 | 2015-01-28 | 河北科星药业有限公司 | Rice bran polysaccharide oral liquid, injection and soluble powder and corresponding preparation method |
| CN107198015A (en) * | 2017-04-24 | 2017-09-26 | 杭州研青生物科技有限公司 | The method that double bacterium semidry method fermentation defatted rice brans obtain active matter |
| CN108478466A (en) * | 2018-06-05 | 2018-09-04 | 艾苛密（上海）健康科技股份有限公司 | Skin moisture-keeping ceramide and preparation method thereof |
| CN113788864A (en) * | 2021-10-23 | 2021-12-14 | 绵阳膳意生物科技有限公司 | Co-production preparation method of ceramide and glucoside (lipid) acyl sphingosine |
-
2009
- 2009-10-12 CN CN2009101533106A patent/CN102041280A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102225161A (en) * | 2011-05-12 | 2011-10-26 | 江南大学 | Method for preparing functional components of antioxidation, blood sugar reduction and cholesterol reduction by using rice bran |
| CN103193893A (en) * | 2013-04-18 | 2013-07-10 | 江南大学 | Preparation method of rice bran polysaccharide sulfate based on ionic liquid |
| CN103193893B (en) * | 2013-04-18 | 2015-04-22 | 江南大学 | Preparation method of rice bran polysaccharide sulfate based on ionic liquid |
| CN104292349A (en) * | 2014-09-16 | 2015-01-21 | 沈阳师范大学 | Equipment and method for preparing carboxymethylated rice bran polysaccharide |
| CN104311437A (en) * | 2014-09-18 | 2015-01-28 | 苏州经贸职业技术学院 | Method for extracting, separating and purifying gamma-aminobutyric acid |
| CN104306391A (en) * | 2014-09-18 | 2015-01-28 | 河北科星药业有限公司 | Rice bran polysaccharide oral liquid, injection and soluble powder and corresponding preparation method |
| CN104311437B (en) * | 2014-09-18 | 2016-03-02 | 苏州经贸职业技术学院 | A kind of extraction separation and purification method of γ-aminobutyric acid |
| CN107198015A (en) * | 2017-04-24 | 2017-09-26 | 杭州研青生物科技有限公司 | The method that double bacterium semidry method fermentation defatted rice brans obtain active matter |
| CN108478466A (en) * | 2018-06-05 | 2018-09-04 | 艾苛密（上海）健康科技股份有限公司 | Skin moisture-keeping ceramide and preparation method thereof |
| CN108478466B (en) * | 2018-06-05 | 2021-03-30 | 艾苛密（上海）健康科技股份有限公司 | Ceramide for moisturizing skin and preparation method thereof |
| CN113788864A (en) * | 2021-10-23 | 2021-12-14 | 绵阳膳意生物科技有限公司 | Co-production preparation method of ceramide and glucoside (lipid) acyl sphingosine |
| CN113788864B (en) * | 2021-10-23 | 2024-09-13 | 绵阳膳意生物科技有限公司 | Co-production preparation method of ceramide and glucoside (lipid) sphingosine |
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Application publication date: 20110504 |