CN104172353B - Giant salamander polypeptide beverage and preparation method thereof - Google Patents
Giant salamander polypeptide beverage and preparation method thereof Download PDFInfo
- Publication number
- CN104172353B CN104172353B CN201410404611.2A CN201410404611A CN104172353B CN 104172353 B CN104172353 B CN 104172353B CN 201410404611 A CN201410404611 A CN 201410404611A CN 104172353 B CN104172353 B CN 104172353B
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- Prior art keywords
- giant salamander
- polypeptide powder
- salamander polypeptide
- liquid
- beverage
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 141
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 137
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 121
- 235000013361 beverage Nutrition 0.000 title claims abstract description 56
- 238000002360 preparation method Methods 0.000 title claims abstract description 41
- 241000157862 Dicamptodontidae Species 0.000 title abstract 8
- 239000007788 liquid Substances 0.000 claims abstract description 78
- 239000000796 flavoring agent Substances 0.000 claims abstract description 32
- 235000019634 flavors Nutrition 0.000 claims abstract description 32
- 235000000346 sugar Nutrition 0.000 claims abstract description 26
- 235000015203 fruit juice Nutrition 0.000 claims abstract description 24
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims abstract description 21
- 239000004302 potassium sorbate Substances 0.000 claims abstract description 21
- 235000010241 potassium sorbate Nutrition 0.000 claims abstract description 21
- 229940069338 potassium sorbate Drugs 0.000 claims abstract description 21
- 239000000230 xanthan gum Substances 0.000 claims abstract description 19
- 235000010493 xanthan gum Nutrition 0.000 claims abstract description 19
- 229920001285 xanthan gum Polymers 0.000 claims abstract description 19
- 229940082509 xanthan gum Drugs 0.000 claims abstract description 19
- 241001415306 Cryptobranchidae Species 0.000 claims description 101
- 239000000843 powder Substances 0.000 claims description 100
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- 241001253208 Andrias davidianus Species 0.000 claims description 62
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 51
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- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 16
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 16
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 15
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- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims description 5
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- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 2
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 claims description 2
- 229910052700 potassium Inorganic materials 0.000 claims description 2
- 239000011591 potassium Substances 0.000 claims description 2
- 239000004334 sorbic acid Substances 0.000 claims description 2
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- 230000036541 health Effects 0.000 abstract description 5
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 abstract description 4
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- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 abstract 1
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- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 2
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- LGJMUZUPVCAVPU-UHFFFAOYSA-N beta-Sitostanol Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(C)CCC(CC)C(C)C)C1(C)CC2 LGJMUZUPVCAVPU-UHFFFAOYSA-N 0.000 description 1
- ORQIZUYAGXZVPI-UHFFFAOYSA-N bilobanone Natural products O1C(CC(C)C)=CC(C2CC(=O)C(C)=CC2)=C1 ORQIZUYAGXZVPI-UHFFFAOYSA-N 0.000 description 1
- CPGCVOVWHCWVTP-UHFFFAOYSA-N celidoniol Natural products CCCCCCCCCCCCCCCCCCCC(O)CCCCCCCCC CPGCVOVWHCWVTP-UHFFFAOYSA-N 0.000 description 1
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- HCXVJBMSMIARIN-PHZDYDNGSA-N stigmasterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)/C=C/[C@@H](CC)C(C)C)[C@@]1(C)CC2 HCXVJBMSMIARIN-PHZDYDNGSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/66—Proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The invention discloses a beverage or a health beverage, in particular relates to a giant salamander polypeptide beverage and a preparation method thereof, and belongs to the technical fields of giant salamander deep processing, food, health products and medicines. The giant salamander polypeptide beverage comprises 5%-15% of sugar, 0.05%-0.15% of beta-cyclodextrin, 0.05%-0.15% of xanthan gum, 0.01%-0.025% of potassium sorbate, 0.15%-0.4% of tartaric acid, 3%-10% of fruit juice and the balance being a giant salamander polypeptide liquid. The giant salamander polypeptide beverage is uniformly golden yellow or pale yellow, looks like a semitransparent uniform liquid, is free of the peculiar unpleasant smell of giant salamander polypeptide by use of the components of the formula, and has the advantages of good taste and flavor, high nutritional value, environmental friendliness and safety, and the good-quality giant salamander polypeptide can be supplemented conveniently in daily life.
Description
Technical field
The present invention relates to a kind of beverage or health promoting beverage, especially a kind of giant salamander polypeptide powder beverage and preparation method thereof, belong to
Andrias davidianus Blanchard deep processing and food, health product and pharmaceutical technology field.
Background technology
Andrias davidianus Blanchard is the animal that a kind of edibility is high, its fine and tender taste, delicious flavour, containing high quality protein, enriches
Aminoacid and trace element, high nutritive value, be described as " Radix Ginseng in water ", Andrias davidianus Blanchard muscle protein is a kind of high-quality protein,
Essential amino acids content is high, and proportion of composing is good, complies fully with human body requirement pattern, its nutritive value be much better than Carnis Haliotidis, Nidus collocaliae,
Fin Mustetus manazo and Trionyx sinensis Wiegmann.Andrias davidianus Blanchard muscle protein is rich in 18 kinds of aminoacid, and wherein 6 kinds of delicious amino acid account for the 42.77% of total amino acid content,
8 kinds of essential amino acids account for the 40.72% of total amino acid content, it is necessary to aminoacid and non essential amino acid ratio are 68.68%,
All meet the idealized model of FAO/WHO；Andrias davidianus Blanchard essential amino acids scoring height, meets human body requirement pattern degree at a relatively high, its
In containing the lysine easily lacked in abundant China's staple food, can be reasonably combined with staple food edible, mutual to play protein
Benefit effect.
The eating method of artificially-cultured giant salamander mainly uses the mode of conventional cooking, and system is inhaled in the digestion being limited to human body
System so that the nutritive value of Andrias davidianus Blanchard can not be fully utilized.Along with the development of biotechnology, occur in that and Andrias davidianus Blanchard is carried out deeply
Processing technique.
Summary of the invention
The goal of the invention of the present invention is: provide a kind of giant salamander polypeptide powder be raw material there is health care beverage, improve big
The absorption of salamander polypeptide, the most this beverage has local flavor and the mouthfeel of uniqueness, giant salamander polypeptide powder can be overcome to have foreign odor, energy
Enough accepted by consumers in general.
The technical solution used in the present invention is as follows:
A kind of giant salamander polypeptide powder beverage, comprises giant salamander polypeptide powder liquid, sugar, beta-schardinger dextrin-, xanthan gum, potassium sorbate, fruit acid and fruit
Juice, each constituent mass mark is sugar 5-15%, beta-schardinger dextrin-0.05-0.15%, xanthan gum 0.05-0.15%, potassium sorbate
0.01-0.025%, fruit acid 0.15-0.4%, fruit juice 3-10%, remaining is giant salamander polypeptide powder liquid.
This giant salamander polypeptide powder beverage also includes the ginkgo extract of 0.5-5% and the Herba Taraxaci extract of 0.5-1%.
Described fruit acid is made up of for 3-6:1 with mass ratio citric acid and malic acid, and described fruit juice is Sucus Mali pumilae；Described fruit
Juice is Passion Fruit Juice.
Each constituent mass mark sugar 10%, beta-schardinger dextrin-1%, xanthan gum 0.1%, potassium sorbate in giant salamander polypeptide powder beverage
0.02%, citric acid 0.25%, malic acid 0.05%, Sucus Mali pumilae 5%, remaining is giant salamander polypeptide powder liquid.
Each constituent mass mark sugar 10%, beta-schardinger dextrin-1%, xanthan gum 0.1%, potassium sorbate in giant salamander polypeptide powder beverage
0.02%, citric acid 0.25%, malic acid 0.05%, white Oleum Linderae juice 5%, remaining is giant salamander polypeptide powder liquid.
Giant salamander polypeptide powder beverage each constituent mass mark sugar 10%, beta-schardinger dextrin-1%, xanthan gum 0.1%, potassium sorbate
0.02%, citric acid 0.25%, malic acid 0.05%, white Oleum Linderae juice 5%, ginkgo extract 3%, Herba Taraxaci extract 0.6%,
Remaining is giant salamander polypeptide powder liquid.
In described giant salamander polypeptide powder beverage, content of peptides is not less than 0.02%, and surplus is water.
The molecular weight of described giant salamander polypeptide powder liquid is not more than 20000Da.
The preparation method of a kind of giant salamander polypeptide powder beverage, comprises the steps of
(1) be dissolved in water saccharogenesis water filtration remove impurity by raw material sugar；
(2) beta-schardinger dextrin-, xanthan gum, potassium sorbate, fruit acid are incorporated stirring and dissolving in appropriate water, add sucrose solution,
Giant salamander polypeptide powder liquid and fruit juice, by deployed product liquid filtering and impurity removing, control content of peptides in giant salamander polypeptide powder beverage and be not less than
0.02%；
(3) stock solution after filtering uniformly is mixed by colloid mill, obtains uniform and stable product liquid；
(4) product liquid is carried out fill and sterilization forms.
A couple of days of being stopped eating by work Andrias davidianus Blanchard makes it empty the intestine, then slaughters gallbladder, entirely will be used for the preparation of giant salamander polypeptide powder by tissue.
The preparation method of a kind of giant salamander polypeptide powder beverage, it is also possible in step (2), by beta-schardinger dextrin-, xanthan gum, sorbic acid
Potassium, fruit acid, ginkgo extract and Herba Taraxaci extract incorporate stirring and dissolving in appropriate water, add sucrose solution, giant salamander polypeptide powder liquid
And fruit juice, by deployed product liquid filtering and impurity removing, control content of peptides in giant salamander polypeptide powder beverage and be not less than 0.02%.
Described giant salamander polypeptide powder liquid prepares as follows:
(a) pretreatment: Andrias davidianus Blanchard is cleaned and stripping and slicing；
B () infusion: add water to gained Andrias davidianus Blanchard cube meat, infusion to Megalobatrachus japonicus daoidianuas(Blanchard) block organization softens；
(c) enzymolysis: after the feed liquid cooling after infusion, moisturizing regulation solid-liquid ratio, add compound protease and carry out enzymolysis,
Described compound protease includes neutral protease, papain and flavor protease composition, and described flavor protease is by ammonia peptide
Enzyme and carboxypeptidase composition；
(d) enzyme denaturing: enzymolysis solution is boiled enzyme denaturing after completing by enzymolysis；
E () filters: the feed liquid after enzyme denaturing is carried out multistage filtering, the pure polypeptide products of system.
In described step (a), Andrias davidianus Blanchard is cut into 0.5-2cm3Block.
In described step (c), described neutral protein enzyme dosage is the 0.5%-1.5% of Andrias davidianus Blanchard cube meat quality, Papain
Enzyme dosage is the 0.6%-2.0% of Andrias davidianus Blanchard cube meat quality；Described flavor protease by aminopeptidase and carboxypeptidase with mass ratio 3-5:1
Composition, flavor protease consumption is the 0.3-1.0% of Andrias davidianus Blanchard cube meat quality.Preferably, described neutral protein enzyme dosage is Andrias davidianus Blanchard
The 1.2% of cube meat quality, Papain enzyme dosage is the 1.6% of Andrias davidianus Blanchard cube meat quality, described flavor protease by aminopeptidase and
Carboxypeptidase forms with mass ratio 4:1, and flavor protease consumption is the 0.8% of Andrias davidianus Blanchard cube meat quality.Adjust in described step (c) moisturizing
In joint solid-liquid ratio, regulation Andrias davidianus Blanchard cube meat is 1:2-6 with the ratio of water, uses the mode of stirring to carry out enzymolysis, and mixing speed is
400r/min, hydrolysis temperature is 45 DEG C-55 DEG C, enzymolysis time is 4-8 hour, and enzymolysis PH is 6.5-7.5.
In described step in (e), use three grades of step bore filter, at different levels be followed successively by 30-50 μm, 5 μm, 0.45 μm,
Rear employing interception is that the ultrafilter membrane of 20000Da carries out ultrafiltration.
Described giant salamander polypeptide powder liquid can also prepare as follows:
A Andrias davidianus Blanchard cube meat is cleaned by (), stripping and slicing, adds the aseptic ultra-pure water of 3 times of volumes, makes homogenate, add 3-5 times of body
Long-pending aseptic ultra-pure water, mix homogeneously；
B the pH value of () regulation homogenate is to 3-4, in-30 DEG C～-10 DEG C of multigelations 3-8 time, after last freeze thawing
Be homogenized 5-10 DEG C through 2 times, each 10-15 minute, rotating speed be the centrifugal of 6000r/min and filter, take supernatant；
C () regulation supernatant pH value, to 5.5-6.5, filters with ultrafilter membrane, obtains giant salamander polypeptide powder liquid.
In described step (b), after centrifugal filtration, supernatant is heated 10 minutes in 60-70 DEG C for the first time, is cooled to room temperature,
Carry out second time more centrifugal.
In described step (c), use three grades of step bore filter, 30-50 μm, 5 μm, 0.45 μm of being followed successively by different levels, finally
The ultrafilter membrane using interception to be 10000Da carries out ultrafiltration.
The preparation technology of the giant salamander polypeptide powder liquid being prepared is simple and stable, through multigelation, makes cell breakage, enters
And discharge polypeptide more.Use purely physical operation preparation, be added without other composition, the purest more healthy, and can be
The activity preserving polypeptide of limits.Use two times centrifugal and filtration, coordinate ultrafilter membrane, it is possible to obtain no more than 10000Da's
Polypeptide extract, more conducively absorption of human body.
The preparation method of described Passion Fruit Juice is:
(1) select more than 95% well-done Passifolra edulis, after removing peel, obtain Passifolra edulis sarcocarp, then add appropriate water and exist
Temperature carries out infusion 30 minutes under conditions of being 80-95 DEG C, then filters, and then in vacuum 0.08Mpa, temperature is 60
Vacuum impregnation 25min under conditions of DEG C, then dipping 2-4 hour at ambient pressure, obtain Passifolra edulis natural juice；
(2) in terms of the weight portion of Passifolra edulis sarcocarp, by crystal sugar 20-40 part, high fructose syrup 5-30 part, brown sugar 0.5-1.5 part
Putting with water 30-150 part and be heated to 70-90 DEG C in pot, stirring is to melting completely；
(3) solution obtained in the Passifolra edulis natural juice of gained in step (1) and step (2) is mixed, obtain Passifolra edulis
Juice.In parts by weight, 150 parts of water in Passifolra edulis sarcocarp 80 parts, step (1), crystal sugar 20-40 part, high fructose syrup 5-30 part, red
Water 30-150 part in sugar 0.5-1.5 part, step (2).
The preparation method of described ginkgo extract is:
(1) take fresh Folium Ginkgo, then make its normal temperature drying 3-5 days under field conditions (factors), then it is added in iron pan
Water carries out infusion, and the temperature of infusion is 80-95 DEG C, and the time is 20-50 minute, then filters；Obtain the extracting solution of Folium Ginkgo；
In parts by weight, fresh Folium Ginkgo is 50～100 parts；The addition of water is 200-300 part.
(2) commercially available Semen Ginkgo fruit is taken so that it is carry out pulverizing 200 mesh sieves by fruit after Po Sui, obtain Semen Ginkgo sarcocarp；Then
Carrying out infusion together with being added water by the leaf slag of the shell of Semen Ginkgo fruit with the Folium Ginkgo obtained in step (1), the temperature of infusion is 90-95
DEG C, the time is 3-5h, then filters, and obtains filtrate；The weight portion of Semen Ginkgo fruit is 20-40 part, and the weight portion of water is 120-160
Part.
(3) the filtrate mixing that will obtain in the filtrate obtained in step (1) and step (2), is subsequently adding the Semen Ginkgo of pulverizing
Fruit, and white sugar mixes, and obtains ginkgo extract.In parts by weight, the weight portion of white sugar is 30-60 part.
Described Herba Taraxaci extract dandelion root fluidextract.
In sum, owing to have employed technique scheme, the invention has the beneficial effects as follows: the giant salamander polypeptide powder drink of the present invention
Product rich in nutritive value, it is possible to utilize each component of formula itself to overcome the special foreign odor of giant salamander polypeptide powder, there is mouthfeel
With raciness, be of high nutritive value, the advantage of green safety, facilitate the daily high-quality polypeptide to Andrias davidianus Blanchard to supplement；Use described
The giant salamander polypeptide powder of method, it is possible to being prevented effectively from Andrias davidianus Blanchard horny layer of epidermis affects enzymolysis process and enzymatic hydrolysate, enzymatic hydrolysis condition
Gentleness, can carry out sufficient enzymolysis to Megalobatrachus japonicus daoidianuas(Blanchard), and enzymolysis process is easily controllable, enzymatic hydrolysate safety and beneficially absorption of human body.With
The giant salamander polypeptide powder beverage that it is prepared can be with relieving alcoholism and protecting livers, diuresis, strengthening the spleen and stomach.
Detailed description of the invention
All features disclosed in this specification, or disclosed all methods or during step, except mutually exclusive
Feature and/or step beyond, all can combine by any way.
Any feature disclosed in this specification, unless specifically stated otherwise, all can by other equivalence or there is similar purpose
Alternative features is replaced.I.e., unless specifically stated otherwise, an example during each feature is a series of equivalence or similar characteristics
?.A couple of days of being stopped eating by work Andrias davidianus Blanchard makes it empty the intestine, then slaughters gallbladder, entirely will be used for the preparation of giant salamander polypeptide powder by tissue.
Embodiment 1
Prepared by giant salamander polypeptide powder: (a) pretreatment: cleaned by Andrias davidianus Blanchard and be cut into 0.5-2cm3Bulk (b) infusion: big to gained
Salamander cube meat adds water, infusion 20min-30min at 111 DEG C-123 DEG C, softens to Megalobatrachus japonicus daoidianuas(Blanchard) block organization；(c) enzymolysis: after infusion
Feed liquid natural cooling with pressure after, regulate material-water ratio as 1:2 with the quality moisturizing of raw material Andrias davidianus Blanchard cube meat, add by neutral protein
The compound protease of enzyme, papain and flavor protease composition carries out enzymolysis, and described neutral protease is 50,000 U/g, consumption
For the 0.5% of Andrias davidianus Blanchard cube meat quality, papain is 100,000 U/g, and consumption is the 0.6% of Andrias davidianus Blanchard cube meat quality, local flavor albumen
Enzyme is made up of with mass ratio 3:1 aminopeptidase and carboxypeptidase, and aminopeptidase and carboxypeptidase are 1.5 ten thousand U/g, and flavor protease consumption is
The 0.3% of Andrias davidianus Blanchard cube meat quality, enzymolysis uses the mode of stirring to carry out, and mixing speed is 400r/min, hydrolysis temperature is 45 DEG C,
Enzymolysis time is 4 hours, and enzymolysis is to carry out for 6.5 times at PH；D () enzyme denaturing: enzymolysis solution is boiled after completing by enzymolysis, at 90 DEG C-100
15min is inactivated at DEG C；E () filters: the feed liquid after enzyme denaturing carries out multistage filtering, first uses three grades of step bore filter, at different levels
Being followed successively by 30 μm, 5 μm, 0.45 μm, the ultrafilter membrane finally using interception to be 20000Da carries out the pure polypeptide of ultrafiltration and produces
Product.
The preparation of giant salamander polypeptide powder beverage: raw material sugar is dissolved in water saccharogenesis water filtration remove impurity by (1)；(2) by beta-schardinger dextrin-, Huang
Virgin rubber, potassium sorbate, fruit acid incorporate stirring and dissolving in appropriate water, add sucrose solution, giant salamander polypeptide powder liquid and fruit juice, by deployed
Product liquid filtering and impurity removing, controlling content of peptides in giant salamander polypeptide powder beverage is 0.02%；(3) stock solution after filtering passes through colloid
Mill uniformly mixes, and obtains uniform and stable product liquid；(4) product liquid is carried out fill and sterilization forms.
Wherein: described fruit acid is made up of for 3-6:1 with mass ratio citric acid and malic acid.Described fruit juice is Sucus Mali pumilae；Respectively
Constituent mass mark is raw material sugar 5%, beta-schardinger dextrin-0.05%, xanthan gum 0.05%, potassium sorbate 0.01%, citric acid
0.12%, malic acid 0.03%, Sucus Mali pumilae 3%, remaining is giant salamander polypeptide powder liquid, controls giant salamander polypeptide powder by interpolation water in preparation
Content of peptides in beverage, water may come from giant salamander polypeptide powder liquid or raw material components dissolving water.
Embodiment 2
Prepared by giant salamander polypeptide powder: (a) pretreatment: cleaned by Andrias davidianus Blanchard and be cut into 0.5-2cm3Bulk；(b) infusion: to gained
Andrias davidianus Blanchard cube meat adds water, infusion 20min-30min at 111 DEG C-123 DEG C, softens to Megalobatrachus japonicus daoidianuas(Blanchard) block organization；(c) enzymolysis: treat infusion
After feed liquid natural cooling with pressure after, regulate material-water ratio as 1:4 with the quality moisturizing of raw material Andrias davidianus Blanchard cube meat, add by neutral egg
The compound protease of white enzyme, papain and flavor protease composition carries out enzymolysis, and described neutral protease is 50,000 U/g, uses
Amount is the 1.2% of Andrias davidianus Blanchard cube meat quality, and papain is 100,000 U/g, and consumption is the 1.6% of Andrias davidianus Blanchard cube meat quality, local flavor egg
White enzyme is made up of with mass ratio 5:1 aminopeptidase and carboxypeptidase, and aminopeptidase and carboxypeptidase are 1.5 ten thousand U/g, flavor protease consumption
For the 1.0% of Andrias davidianus Blanchard cube meat quality, enzymolysis uses the mode of stirring to carry out, and mixing speed is 400r/min, and hydrolysis temperature is 50
DEG C, enzymolysis time be 6 hours, enzymolysis is to carry out for 7.0 times at PH；(d) enzyme denaturing: enzymolysis solution is boiled after completing by enzymolysis, 90 DEG C-
15min enzyme denaturing is inactivated at 100 DEG C；E () filters: the feed liquid after enzyme denaturing carries out multistage filtering, first uses three grades of step aperture mistakes
Filter, 40 μm, 5 μm, 0.45 μm of being followed successively by different levels, the ultrafilter membrane finally using interception to be 20000Da carries out the pure of ultrafiltration
Polypeptide products.
The preparation of giant salamander polypeptide powder beverage: raw material sugar is dissolved in water saccharogenesis water filtration remove impurity by (1)；(2) by beta-schardinger dextrin-, Huang
Virgin rubber, potassium sorbate, fruit acid incorporate stirring and dissolving in appropriate water, add sucrose solution, giant salamander polypeptide powder liquid and fruit juice, by deployed
Product liquid filtering and impurity removing, controlling content of peptides in giant salamander polypeptide powder beverage is 3%；(3) stock solution after filtering passes through colloid mill
Uniformly mix, obtain uniform and stable product liquid；(4) product liquid is carried out fill and sterilization forms.
Wherein: described fruit acid is made up of for 5:1 with mass ratio citric acid and malic acid.Described fruit juice is Sucus Mali pumilae；Each group
Dividing mass fraction is raw material sugar 10%, beta-schardinger dextrin-1%, xanthan gum 0.1%, potassium sorbate 0.02%, citric acid 0.25%, Herba Marsileae Quadrifoliae
Fruit acid 0.05%, Sucus Mali pumilae 5%, remaining is giant salamander polypeptide powder liquid, controls in giant salamander polypeptide powder beverage many by interpolation water in preparation
Peptide content, water may come from giant salamander polypeptide powder liquid or raw material components dissolving water.
Embodiment 3
Prepared by giant salamander polypeptide powder: (a) pretreatment: cleaned by Andrias davidianus Blanchard and be cut into 0.5-2cm3Bulk；(b) infusion: to gained
Andrias davidianus Blanchard cube meat adds water, infusion 20min-30min at 111 DEG C-123 DEG C, softens to Megalobatrachus japonicus daoidianuas(Blanchard) block organization；(c) enzymolysis: treat infusion
After feed liquid natural cooling with pressure after, regulate material-water ratio as 1:6 with the quality moisturizing of raw material Andrias davidianus Blanchard cube meat, add by neutral egg
The compound protease of white enzyme, papain and flavor protease composition carries out enzymolysis, and described neutral protease is 50,000 U/g, uses
Amount is the 1.5% of Andrias davidianus Blanchard cube meat quality, and papain is 100,000 U/g, and consumption is the 2.0% of Andrias davidianus Blanchard cube meat quality, local flavor egg
White enzyme is made up of with mass ratio 4:1 aminopeptidase and carboxypeptidase, and aminopeptidase and carboxypeptidase are 1.5 ten thousand U/g, flavor protease consumption
For the 0.8% of Andrias davidianus Blanchard cube meat quality, enzymolysis uses the mode of stirring to carry out, and mixing speed is 400r/min, and hydrolysis temperature is 55
DEG C, enzymolysis time be 8 hours, enzymolysis is to carry out for 7.5 times at PH；(d) enzyme denaturing: enzymolysis solution is boiled after completing by enzymolysis, 90 DEG C-
15min enzyme denaturing is inactivated at 100 DEG C；E () filters: the feed liquid after enzyme denaturing carries out multistage filtering, first uses three grades of step aperture mistakes
Filter, 50 μm, 5 μm, 0.45 μm of being followed successively by different levels, the ultrafilter membrane finally using interception to be 20000Da carries out the pure of ultrafiltration
Polypeptide products.
The preparation of giant salamander polypeptide powder beverage: raw material sugar is dissolved in water saccharogenesis water filtration remove impurity by (1)；(2) by beta-schardinger dextrin-, Huang
Virgin rubber, potassium sorbate, fruit acid incorporate stirring and dissolving in appropriate water, add sucrose solution, giant salamander polypeptide powder liquid and fruit juice, by deployed
Product liquid filtering and impurity removing, controlling content of peptides in giant salamander polypeptide powder beverage is 3.5%；(3) stock solution after filtering passes through colloid
Mill uniformly mixes, and obtains uniform and stable product liquid；(4) product liquid is carried out fill and sterilization forms.
Wherein: described fruit acid is made up of for 3:1 with mass ratio citric acid and malic acid.Described fruit juice is Sucus Mali pumilae；Each group
Dividing mass fraction is raw material sugar 15%, beta-schardinger dextrin-0.15%, xanthan gum 0.15%, potassium sorbate 0.025%, citric acid
0.3%, malic acid 0.1%, apple juice 10%, remaining is giant salamander polypeptide powder liquid, controls Andrias davidianus Blanchard by interpolation water many in preparation
Content of peptides in peptide beverage, water may come from giant salamander polypeptide powder liquid or raw material components dissolving water.
In the above-described embodiments, the preparation process (4) of giant salamander polypeptide powder beverage uses ultrahigh-pressure sterilization, temperature 121 DEG C, pressure
Power 0.11Mpa, sterilizing time 10min.
The giant salamander polypeptide powder beverage obtained in embodiment 1-embodiment 3 is analyzed, embodiment 1-embodiment 3 obtains
Giant salamander polypeptide powder beverage color and luster is the golden yellow of uniformity or light yellow, and outward appearance is translucent the liquid of uniformity, has Herba Marsileae Quadrifoliae
Fruit juice and the intrinsic fragrance of Andrias davidianus Blanchard enzymolysis solution and flavour body.
Embodiment 4:
Prepared by giant salamander polypeptide powder: (a) pretreatment: cleaned by Andrias davidianus Blanchard and be cut into 0.5-2cm3Bulk；(b) infusion: to gained
Andrias davidianus Blanchard cube meat adds water, infusion 20min-30min at 111 DEG C-123 DEG C, softens to Megalobatrachus japonicus daoidianuas(Blanchard) block organization；(c) enzymolysis: treat infusion
After feed liquid natural cooling with pressure after, regulate material-water ratio as 1:2 with the quality moisturizing of raw material Andrias davidianus Blanchard cube meat, add by neutral egg
The compound protease of white enzyme, papain and flavor protease composition carries out enzymolysis, and described neutral protease is 50,000 U/g, uses
Amount is the 0.5% of Andrias davidianus Blanchard cube meat quality, and papain is 100,000 U/g, and consumption is the 0.6% of Andrias davidianus Blanchard cube meat quality, local flavor egg
White enzyme is made up of with mass ratio 3:1 aminopeptidase and carboxypeptidase, and aminopeptidase and carboxypeptidase are 1.5 ten thousand U/g, flavor protease consumption
For the 0.3% of Andrias davidianus Blanchard cube meat quality, enzymolysis uses the mode of stirring to carry out, and mixing speed is 400r/min, and hydrolysis temperature is 45
DEG C, enzymolysis time be 4 hours, enzymolysis is to carry out for 6.5 times at PH；(d) enzyme denaturing: enzymolysis solution is boiled after completing by enzymolysis, 90 DEG C-
15min is inactivated at 100 DEG C；E () filters: the feed liquid after enzyme denaturing carries out multistage filtering, first uses three grades of step bore filter, respectively
Level is followed successively by 30 μm, 5 μm, 0.45 μm, and the ultrafilter membrane finally using interception to be 20000Da carries out the pure polypeptide of ultrafiltration
Product.
The preparation of giant salamander polypeptide powder beverage: raw material sugar is dissolved in water saccharogenesis water filtration remove impurity by (1)；(2) by beta-schardinger dextrin-, Huang
Virgin rubber, potassium sorbate, fruit acid, ginkgo extract and Passion Fruit Juice incorporate stirring and dissolving in appropriate water, add sucrose solution, Andrias davidianus Blanchard
Polypeptide liquid and fruit juice, by deployed product liquid filtering and impurity removing, controlling content of peptides in giant salamander polypeptide powder beverage is 0.35%；(3)
Stock solution after filtering uniformly is mixed by colloid mill, obtains uniform and stable product liquid；(4) product liquid is carried out fill
Form with sterilization.
Wherein: described fruit acid is made up of for 3:1 with mass ratio citric acid and malic acid.Described fruit juice is Passion Fruit Juice；Respectively
Constituent mass mark is raw material sugar 15%, beta-schardinger dextrin-0.15%, xanthan gum 0.15%, potassium sorbate 0.025%, citric acid
0.3%, malic acid 0.1%, Passion Fruit Juice 10%, ginkgo extract 3%, Herba Taraxaci extract 0.6%, remaining is giant salamander polypeptide powder
Liquid, controls content of peptides in giant salamander polypeptide powder beverage by adding water in preparation, and water may come from giant salamander polypeptide powder liquid or former
Material component dissolving water.
In the above-described embodiments, the preparation process (4) of giant salamander polypeptide powder beverage uses ultrahigh-pressure sterilization, temperature 121 DEG C, pressure
Power 0.11Mpa, sterilizing time 10min.
The giant salamander polypeptide powder beverage color and luster using system of the present invention is the golden yellow of uniformity or light yellow, and outward appearance is translucent
The liquid of uniformity, has Sucus Mali pumilae and the intrinsic fragrance of Andrias davidianus Blanchard enzymolysis solution and flavour body.
The preparation method of above-mentioned Passion Fruit Juice is:
(1) select more than 95% well-done Passifolra edulis, after removing peel, obtain Passifolra edulis sarcocarp, then add appropriate water and exist
Temperature carries out infusion 30 minutes under conditions of being 80-95 DEG C, then filters, and then in vacuum 0.08Mpa, temperature is 60
Vacuum impregnation 25min under conditions of DEG C, then dipping 2-4 hour at ambient pressure, obtain Passifolra edulis natural juice；
(2) in terms of the weight portion of Passifolra edulis sarcocarp, by crystal sugar 20-40 part, high fructose syrup 5-30 part, brown sugar 0.5-1.5 part
Putting with water 30-150 part and be heated to 70-90 DEG C in pot, stirring is to melting completely；
(3) solution obtained in the Passifolra edulis natural juice of gained in step (1) and step (2) is mixed, obtain Passifolra edulis
Juice.In parts by weight, 150 parts of water in Passifolra edulis sarcocarp 80 parts, step (1), crystal sugar 20-40 part, high fructose syrup 5-30 part, red
Water 30-150 part in sugar 0.5-1.5 part, step (2).
The preparation method of described ginkgo extract is:
(3) take fresh Folium Ginkgo, then make its normal temperature drying 3-5 days under field conditions (factors), then it is added in iron pan
Water carries out infusion, and the temperature of infusion is 80-95 DEG C, and the time is 20-50 minute, then filters；Obtain the extracting solution of Folium Ginkgo；
In parts by weight, fresh Folium Ginkgo is 50～100 parts；The addition of water is 200-300 part.
(4) commercially available Semen Ginkgo fruit is taken so that it is carry out pulverizing 200 mesh sieves by fruit after Po Sui, obtain Semen Ginkgo sarcocarp；Then
Carrying out infusion together with being added water by the leaf slag of the shell of Semen Ginkgo fruit with the Folium Ginkgo obtained in step (1), the temperature of infusion is 90-95
DEG C, the time is 3-5h, then filters, and obtains filtrate；The weight portion of Semen Ginkgo fruit is 20-40 part, and the weight portion of water is 120-160
Part.(3) the filtrate mixing that will obtain in the filtrate obtained in step (1) and step (2), is subsequently adding the Semen Ginkgo fruit of pulverizing,
And white sugar mixes, obtain ginkgo extract.In parts by weight, the weight portion of white sugar is 30-60 part.
Embodiment 5:
The preparation of giant salamander polypeptide powder is with embodiment 1, the preparation of giant salamander polypeptide powder beverage: raw material sugar is dissolved in water into sucrose solution by (1)
Filtering and impurity removing；(2) beta-schardinger dextrin-, xanthan gum, potassium sorbate, fruit acid, ginkgo extract and Passion Fruit Juice are incorporated appropriate water
Middle stirring and dissolving, adds sucrose solution, giant salamander polypeptide powder liquid and fruit juice, by deployed product liquid filtering and impurity removing, controls giant salamander polypeptide powder
In beverage, content of peptides is 0.30%；(3) stock solution after filtering uniformly is mixed by colloid mill, obtains uniform and stable
Product liquid；(4) product liquid is carried out fill and sterilization forms.
Wherein: described fruit acid is made up of for 3:1 with mass ratio citric acid and malic acid.Described fruit juice is Passion Fruit Juice；Respectively
Constituent mass mark is raw material sugar 15%, beta-schardinger dextrin-0.15%, xanthan gum 0.15%, potassium sorbate 0.025%, citric acid
0.3%, malic acid 0.1%, Passion Fruit Juice 10%, ginkgo extract 4%, Herba Taraxaci extract 0.8%, remaining is giant salamander polypeptide powder
Liquid, controls content of peptides in giant salamander polypeptide powder beverage by adding water in preparation, and water may come from giant salamander polypeptide powder liquid or former
Material component dissolving water.
The preparation method of Passion Fruit Juice and ginkgo extract is with embodiment 4.
Embodiment 6:
The preparation of giant salamander polypeptide powder is with embodiment 1, the preparation of giant salamander polypeptide powder beverage: raw material sugar is dissolved in water into sucrose solution by (1)
Filtering and impurity removing；(2) beta-schardinger dextrin-, xanthan gum, potassium sorbate, fruit acid, ginkgo extract and Passion Fruit Juice are incorporated appropriate water
Middle stirring and dissolving, adds sucrose solution, giant salamander polypeptide powder liquid and fruit juice, by deployed product liquid filtering and impurity removing, controls giant salamander polypeptide powder
In beverage, content of peptides is 0.35%；(3) stock solution after filtering uniformly is mixed by colloid mill, obtains uniform and stable
Product liquid；(4) product liquid is carried out fill and sterilization forms.
Wherein: described fruit acid is made up of for 3:1 with mass ratio citric acid and malic acid.Described fruit juice is Passion Fruit Juice；Respectively
Constituent mass mark is raw material sugar 15%, beta-schardinger dextrin-0.15%, xanthan gum 0.15%, potassium sorbate 0.025%, citric acid
0.3%, malic acid 0.1%, Passion Fruit Juice 10%, ginkgo extract 5%, Herba Taraxaci extract 1%, remaining is giant salamander polypeptide powder
Liquid, controls content of peptides in giant salamander polypeptide powder beverage by adding water in preparation, and water may come from giant salamander polypeptide powder liquid or former
Material component dissolving water.
The preparation method of Passion Fruit Juice and ginkgo extract is with embodiment 4.
Being analyzed the giant salamander polypeptide powder beverage obtained in embodiment 1-embodiment 6, the Andrias davidianus Blanchard using system of the present invention is many
Peptide beverage color and luster is the golden yellow of uniformity or light yellow, and outward appearance is translucent the liquid of uniformity, has Passion Fruit Juice
Fragrance intrinsic with Andrias davidianus Blanchard enzymolysis solution and flavour body, more owing to adding ginkgo extract and Passion Fruit Juice；And appropriate Herba Taraxaci
Extract, makes the giant salamander polypeptide powder beverage rich in nutritive value of the present invention, it is possible to utilize each component of formula itself to overcome Andrias davidianus Blanchard
The special foreign odor of polypeptide, has that taste and flavor is good, is of high nutritive value, the advantage of green safety, facilitates daily to Andrias davidianus Blanchard
High-quality polypeptide supplement；Use the giant salamander polypeptide powder of described method, it is possible to be prevented effectively from Andrias davidianus Blanchard horny layer of epidermis to enzymolysis
Process and enzymatic hydrolysate impact, enzymatic hydrolysis condition is gentle, Megalobatrachus japonicus daoidianuas(Blanchard) can be carried out sufficient enzymolysis, and enzymolysis process is easily controllable, enzyme
Hydrolysis products safety and beneficially absorption of human body.
Comparative example 1:
As a example by embodiment 4, without ginkgo extract on the basis of embodiment 4, remaining preparation method step and reality
Execute example 2 consistent.
Comparative example 2:
As a example by embodiment 4, without giant salamander polypeptide powder raw material on the basis of embodiment 4, remaining preparation method step with
Embodiment 2 is consistent.
By embodiment 1 to embodiment 6, and the beverage obtained in comparative example 1 and comparative example 2 carries out hepatoprotective measure of merit
160 mices, male and female half and half, being randomly divided into 8 groups, often group 20, often organizes 20 including embodiment 1-6, contrast
Example 1-2 often organizes also 20.Each group mice elder generation fasting 12h, the product then preparation all drawn by embodiment 1-6 and comparative example 1-2
Product carry out the gastric infusion of each 20ml, and 1h fills 2 times, then gives 56 degree of Red Star strong, colourless liquor distilled from sorghum Chinese liquor of each group of equal gavage of mice after 1h
0.16ml/10g.Weigh after 12h, gather liver specimens, make coherent detection.ALT, AST are surveyed by test kit description colorimetry
Fixed, ALT and AST concentration in cell is the important indicator of detection hepar damnification.
*P<0.05；
Comparing proof with comparative example 1, embodiment 1, embodiment 2 and embodiment 3 can effectively reduce the liter of ALT, AST
Height, represents that its ALT, AST that can contain that ethanol causes raises, drunk mouse liver injury is had protective effect；Embodiment
4, the effect of embodiment 5 or embodiment 6 is significantly better than embodiment 1, embodiment 2 and embodiment 4 and comparative example 1 and comparative example 2,
Representing the giant salamander polypeptide powder beverage that with the addition of ginkgo extract, the effect of its hepatoprotective is more notable, and interpolation and the Semen Ginkgo of giant salamander polypeptide powder carry
The interpolation taking liquid has more preferable effect.
Comparing proof with comparative example 2, giant salamander polypeptide powder has certain liver protection effect, but DeGrain, only
It is combined with ginkgo extract, could more effectively play the effect of hepatoprotective.
Owing to ginkgo extract containing Ginnol, bilobanone and stigmasterol.Semen Ginkgo is extracted containing 17 kinds of human bodies " required ammonia
Base acid ", also contain trace element necessary to human body；And the lysine content height in giant salamander polypeptide powder, it is raw material with giant salamander polypeptide powder
Develop to meet lysine preparations the most more, but it is inadequate for containing only lysine, also should contain other kind with other
The material of amino acid or human needs is combined.Preparation meets the nutrition enhancer of human nutrition requirement.In this raw material also
Bai Xiangguo juice not only unique flavor, and healthcare, help digest, and has allaying tiredness, relieving alcoholic intoxication of refreshing oneself, antiinflammatory speckle dispelling, beauty treatment
Skin care, prevent the effect such as arteriosclerosis, through overtesting certification, it is also possible to well compound with giant salamander polypeptide powder.
The invention is not limited in aforesaid detailed description of the invention.The present invention expands to any disclose in this manual
New feature or any new combination, and the arbitrary new method that discloses or the step of process or any new combination.
Claims (6)
1. a giant salamander polypeptide powder beverage, it is characterised in that: comprise giant salamander polypeptide powder liquid, sugar, beta-schardinger dextrin-, xanthan gum, potassium sorbate,
Fruit acid and fruit juice, each constituent mass mark is sugar 5-15%, beta-schardinger dextrin-0.05-0.15%, xanthan gum 0.05-0.15%, sorbic acid
Potassium 0.01-0.025%, fruit acid 0.15-0.4%, fruit juice 3-10%, 0.5-5% ginkgo extract, 0.5-1% Herba Taraxaci extract, its
Remaining is giant salamander polypeptide powder liquid, and described fruit acid is made up of for 3-6:1 with mass ratio citric acid and malic acid, described fruit juice be Sucus Mali pumilae or
Bai Xiangguo juice.
2. giant salamander polypeptide powder beverage as claimed in claim 1, it is characterised in that: in described giant salamander polypeptide powder beverage, content of peptides is the lowest
In 2%, the molecular weight of described giant salamander polypeptide powder liquid is not more than 20000Da.
3. the preparation method of giant salamander polypeptide powder beverage as claimed in claim 1, it is characterised in that: comprise the steps of
(1) be dissolved in water saccharogenesis water filtration remove impurity by raw material sugar；
(2) beta-schardinger dextrin-, xanthan gum, potassium sorbate, fruit acid, ginkgo extract and Herba Taraxaci extract are incorporated in appropriate water
Stirring and dissolving, adds sucrose solution, giant salamander polypeptide powder liquid and fruit juice, by deployed product liquid filtering and impurity removing, controls giant salamander polypeptide powder drink
In product, content of peptides is not less than 0.02%；
(3) stock solution after filtering uniformly is mixed by colloid mill, obtains uniform and stable product liquid；
(4) product liquid is carried out fill and sterilization forms.
4. the preparation method of giant salamander polypeptide powder beverage as claimed in claim 3, it is characterised in that: described giant salamander polypeptide powder liquid is by such as
Lower step prepares:
(a) pretreatment: Andrias davidianus Blanchard is cleaned and stripping and slicing；
B () infusion: add water to gained Andrias davidianus Blanchard cube meat, infusion to Megalobatrachus japonicus daoidianuas(Blanchard) block organization softens；
(c) enzymolysis: after the feed liquid cooling after infusion, moisturizing regulation solid-liquid ratio, add compound protease and carry out enzymolysis, described
Compound protease is made up of neutral protease, papain and flavor protease, and described flavor protease is by aminopeptidase and carboxylic
Peptidase forms；
(d) enzyme denaturing: enzymolysis solution is boiled enzyme denaturing after completing by enzymolysis；
E () filters: the feed liquid after enzyme denaturing is carried out multistage filtering, prepares pure polypeptide products.
5. the preparation method of giant salamander polypeptide powder as claimed in claim 4, it is characterised in that: in described step (c), described neutral egg
White enzyme dosage is the 0.5-1.5% of Andrias davidianus Blanchard cube meat quality, and Papain enzyme dosage is the 0.6-2.0% of Andrias davidianus Blanchard cube meat quality；Described
Flavor protease is made up of with mass ratio 3-5:1 aminopeptidase and carboxypeptidase, and flavor protease consumption is Andrias davidianus Blanchard cube meat quality
0.3-1.0%。
6. the preparation method of giant salamander polypeptide powder as claimed in claim 4, it is characterised in that: in described step (c), described neutral egg
White enzyme dosage is the 1.2% of Andrias davidianus Blanchard cube meat quality, and Papain enzyme dosage is the 1.6% of Andrias davidianus Blanchard cube meat quality, described local flavor albumen
Enzyme is made up of with mass ratio 4:1 aminopeptidase and carboxypeptidase, and flavor protease consumption is the 0.8% of Andrias davidianus Blanchard cube meat quality；Described step
In in (e), use three grades of step bore filter, 30-50 μm, 5 μm, 0.45 μm of being followed successively by different levels, finally use the interception to be
The ultrafilter membrane of 20000Da carries out ultrafiltration.
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| CN104432083A (en) * | 2015-01-08 | 2015-03-25 | 四川大学 | Antioxygenic andrias davidianus polypeptide oral solution and preparation method thereof |
| CN106107397A (en) * | 2016-06-27 | 2016-11-16 | 张博 | A kind of Andrias davidianus Blanchard protein peptide powder solid beverage formula and preparation method thereof |
| CN107326056A (en) * | 2017-07-21 | 2017-11-07 | 重庆贡龙大鲵生物科技有限公司 | The preparation method of giant salamander polypeptide powder |
| CN107889989A (en) * | 2017-11-01 | 2018-04-10 | 合阳洽川谷池生物科技研发有限公司 | A kind of giant salamander drink and preparation method thereof |
| CN108042786A (en) * | 2017-12-25 | 2018-05-18 | 高安友 | A kind of compound oral drink of giant salamander polypeptide powder for being used to increase bone density |
| CN109845929A (en) * | 2019-01-23 | 2019-06-07 | 辽宁科技大学 | A kind of mung bean antioxidation polypeptide beverage and preparation method thereof |
| CN110354245A (en) * | 2019-07-16 | 2019-10-22 | 四川省新溪源生物科技发展有限公司 | A kind of composite article and preparation method thereof for treating gout |
| CN110432417A (en) * | 2019-09-03 | 2019-11-12 | 湖北世厚耀榜纪田农业发展有限公司 | A kind of giant salamander protein peptide drinks and preparation method thereof with liver-protecting function |
| CN112293626A (en) * | 2019-11-25 | 2021-02-02 | 重庆康康康生物科技有限公司 | Compound protein bioactive peptide fermented beverage and preparation method thereof |
| CN111034997B (en) * | 2019-12-23 | 2022-11-22 | 张家界（中国）金驰大鲵生物科技有限公司 | Giant salamander active peptide composition with anti-aging, blood pressure regulating and anti-anoxia effects |
| CN112715867B (en) * | 2020-12-28 | 2023-02-03 | 山东蓝奥生物技术有限公司 | Preparation method of flower glue and flower glue |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102090684A (en) * | 2010-09-06 | 2011-06-15 | 山东好当家海洋发展股份有限公司 | Marine collagen peptide beverage and preparation method thereof |
| CN102090693A (en) * | 2010-09-06 | 2011-06-15 | 山东好当家海洋发展股份有限公司 | Oyster polypeptide healthcare beverage and preparation method thereof |
| CN102406205A (en) * | 2011-12-05 | 2012-04-11 | 张家界（中国）金驰大鲵生物科技有限公司 | Oyster polysaccharide fruit juice beverage |
| CN103493969A (en) * | 2013-07-29 | 2014-01-08 | 四川龙王洞生态农业开发有限公司 | Method for producing giant salamander polypeptide powder |
| CN103621869A (en) * | 2013-11-15 | 2014-03-12 | 贵阳学院 | Giant salamander oligosaccharide peptide health-care product |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000308483A (en) * | 1999-04-28 | 2000-11-07 | Yukinobu Toe | Use of microorganism |
-
2014
- 2014-06-16 CN CN201410264789.1A patent/CN104041881A/en active Pending
- 2014-08-15 CN CN201410404611.2A patent/CN104172353B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102090684A (en) * | 2010-09-06 | 2011-06-15 | 山东好当家海洋发展股份有限公司 | Marine collagen peptide beverage and preparation method thereof |
| CN102090693A (en) * | 2010-09-06 | 2011-06-15 | 山东好当家海洋发展股份有限公司 | Oyster polypeptide healthcare beverage and preparation method thereof |
| CN102406205A (en) * | 2011-12-05 | 2012-04-11 | 张家界（中国）金驰大鲵生物科技有限公司 | Oyster polysaccharide fruit juice beverage |
| CN103493969A (en) * | 2013-07-29 | 2014-01-08 | 四川龙王洞生态农业开发有限公司 | Method for producing giant salamander polypeptide powder |
| CN103621869A (en) * | 2013-11-15 | 2014-03-12 | 贵阳学院 | Giant salamander oligosaccharide peptide health-care product |
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