JP2000308483A - Use of microorganism - Google Patents
Use of microorganismInfo
- Publication number
- JP2000308483A JP2000308483A JP11121294A JP12129499A JP2000308483A JP 2000308483 A JP2000308483 A JP 2000308483A JP 11121294 A JP11121294 A JP 11121294A JP 12129499 A JP12129499 A JP 12129499A JP 2000308483 A JP2000308483 A JP 2000308483A
- Authority
- JP
- Japan
- Prior art keywords
- microorganism
- micromonospora
- resistant mutation
- rice extract
- minutes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Non-Alcoholic Beverages (AREA)
- Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、体質改善を図る微
生物の使用方法に関する。[0001] The present invention relates to a method of using a microorganism for improving the constitution.
【０００２】[0002]
【従来の技術と発明が解決しようとする課題】今日、微
生物は多くの産業に利用され、環境や健康等に大きな成
果を上げている。そこで、従来より微生物を使用した各
種方法が提案されている。しかし、考えられる微生物の
多くは気温２０度前後を好み、７０度以上の高温になる
と死滅してしまう。また、化学的に不安定であり、変質
する場合が多い。2. Description of the Related Art Today, microorganisms are used in many industries and have achieved great results in the environment and health. Therefore, various methods using microorganisms have been conventionally proposed. However, many of the conceivable microorganisms prefer temperatures around 20 degrees Celsius and die at temperatures as high as 70 degrees or higher. Further, they are chemically unstable and often deteriorate.
【０００３】本発明は、上記課題を解決するため、体質
改善を図り、健康に優れた微生物の使用方法を提供する
ものである。[0003] In order to solve the above-mentioned problems, the present invention aims to provide a method of using a microorganism which is improved in constitution and is excellent in health.
【０００４】[0004]
【課題を解決するための手段】上記課題は請求項１記載
の発明によれば、こめこめエキスに含まれ、必須アミノ
酸とポリペプチド抽出エキスによって作成され、細胞強
化又は焼酎粕に利用する微生物の使用方法を提供でき
る。According to the first aspect of the present invention, there is provided a microorganism contained in a rice extract, which is prepared from an essential amino acid and a polypeptide extract, and which is used for cell enhancement or shochu lees. How to use can be provided.
【０００５】ここで、上記利用は細胞強化又は焼酎粕に
利用されるだけでなく、果実ジュース等にも利用するこ
とができる。また、請求項２の記載は、上記請求項１記
載の発明において、高温耐性変異操作、及び薬品耐性変
異操作によって耐性変異された単一属のミクロモノスポ
ラを一貫して使用する構成であることを示す。Here, the above-mentioned use can be used not only for cell fortification or shochu lees, but also for fruit juice and the like. Further, the second aspect of the present invention is that, in the first aspect of the present invention, a single genus of Micromonospora resistant to mutation by a high-temperature resistance mutation operation and a drug resistance mutation operation is used consistently. Is shown.
【０００６】また、請求項３の記載は、上記請求項２の
記載において、前記ミクロモノスポラに施す高温耐性変
異操作が、摂氏１００度以上を３０分以上であることを
示し、請求項４の記載は上記請求項７の記載において、
前記ミクロモノスポラに施す薬品耐性変異操作が、所定
濃度のフェノール系殺菌剤中で、３０分以上であること
を示す。According to a third aspect of the present invention, in the second aspect, the high-temperature resistant mutation operation performed on the micromonospora is performed at a temperature of 100 degrees Celsius or more for 30 minutes or more. The description is based on claim 7 described above.
This shows that the drug-resistant mutation operation to be performed on the micromonospora is 30 minutes or more in a phenolic fungicide at a predetermined concentration.
【０００７】尚、請求項５の記載は上記請求項３の記載
において、前記フェノール系殺菌剤が、例えばクレゾー
ル１％水溶液であることを示す。According to a fifth aspect of the present invention, in the third aspect, the phenolic fungicide is, for example, a 1% aqueous solution of cresol.
【０００８】[0008]
【発明の実施の形態】以下、本発明の実施の形態を図面
を参照しながら説明する。本発明で使用する微生物は、
培養又は自然界で通常得られるミクロモノスポラをもと
にして、温度及び薬品に対して耐性変異操作を施したも
のである。Embodiments of the present invention will be described below with reference to the drawings. The microorganism used in the present invention is
This is a micromonospora commonly obtained in culture or in nature, and subjected to a temperature and drug resistance mutation procedure.
【０００９】例えば、富栄養化した河川から取り出した
汚泥を１ｍｍのメッシュ、及び濾紙で濾過し、水気を切
って自然乾燥を行う。そして、その中の汚泥だけを抽出
し、シャーレに入れた状態で高温滅菌機を使用し、摂氏
１００度の温度で３０分間耐性変異操作を行う。For example, sludge taken out from a eutrophic river is filtered with a 1 mm mesh and filter paper, drained, and air-dried. Then, only the sludge therein is extracted, and a resistance mutation operation is performed at a temperature of 100 degrees Celsius for 30 minutes using a high-temperature sterilizer in a state of being placed in a petri dish.
【００１０】その後、冷えてから蒸留水で１％に希釈し
たフェノール系殺菌剤の一種であるクレゾール水溶液中
に３０分以上浸し、耐性変異操作を行う。その後、再度
濾紙での濾過及び自然乾燥を行い、黒糖を加えた培地で
培養させ、選別分離により目的とするミクロモノスポラ
を得る。Then, after cooling, the plate is immersed in a cresol aqueous solution, which is a kind of phenolic fungicide diluted to 1% with distilled water, for 30 minutes or more to perform a resistance mutation operation. Thereafter, the mixture is again filtered through filter paper and air-dried, cultured in a medium supplemented with brown sugar, and separated and separated to obtain a desired micromonospora.
【００１１】さらに、上記のようにして得たミクロモノ
スポラに対し、目的とした菌を培養する。例えば、こめ
こめエキスであり、上記各種菌の作成は、紫外線やオゾ
ンなどの刺激を与えて突然変異を起こさせるものではな
く、スクリーニング（選別）法や効果的な増殖方法等の
変異操作によって得たものである。[0011] Further, a desired bacterium is cultured on the micromonospora obtained as described above. For example, it is a rice extract, and the above-mentioned various bacteria are not produced by applying a stimulus such as ultraviolet rays or ozone to cause mutation, but are obtained by a mutation operation such as a screening (selection) method or an effective growth method. It is a thing.
【００１２】本例のこめこめエキスは、前述の耐性変異
操作を行い得られた菌を使用し、ジュース等の飲み物に
含ませたものである。また、こめこめエキスは必須アミ
ノ酸とポリペプチド抽出エキスによって製造され、こめ
こめエキスを含むジュース等の飲み物を飲むことによっ
て、細胞の強化を図ることができる。また、こめこめエ
キスを焼酎粕に利用してもよい。The rice extract of the present example is obtained by using bacteria obtained by performing the above-mentioned resistance mutation operation and adding it to a drink such as juice. In addition, the rice extract is produced using an essential amino acid and a polypeptide extract, and cells can be strengthened by drinking a drink such as a juice containing the rice extract. Moreover, the rice extract may be used for shochu lees.
【００１３】尚、本例で使用する耐性菌を含まないが利
用価値が大きいエキスとしては、コヘンルーダエキス、
琉球ヨモギエキス、水虫シャンプー等がある。The extracts which do not contain resistant bacteria and have high utility value used in this example include Kohenruda extract,
Ryukyu mugwort extract, athlete's foot shampoo, etc.
【００１４】[0014]
【発明の効果】以上説明したように、本発明のこめこめ
エキスは細胞強化等の効能があり、極めて有用な微生物
の利用方法である。As described above, the rice extract of the present invention has an effect such as cell strengthening and is an extremely useful method for utilizing microorganisms.
Claims (5)
とポリペプチド抽出エキスによって作成され、細胞強化
又は焼酎粕に利用する微生物の使用方法。1. A method of using a microorganism which is contained in a rice extract and is produced by an essential amino acid and polypeptide extract, and is used for cell enhancement or shochu lees.
作、及び薬品耐性変異操作によって耐性変異された単一
属のミクロモノスポラを一貫して使用することを特徴と
する請求項１記載の微生物の使用方法。2. The microorganism according to claim 1, wherein the rice extract comprises a single genus of Micromonospora which has been mutated by high-temperature-resistant mutation and drug-resistant mutation. How to use
異操作が、摂氏１００度以上を３０分以上であることを
特徴とする請求項２記載の微生物の使用方法。3. The method according to claim 2, wherein the high-temperature resistant mutation operation performed on the micromonospora is carried out at 100 ° C. or more for 30 minutes or more.
異操作が、所定濃度のフェノール系殺菌剤中で、３０分
以上であることを特徴とする請求項２記載の微生物の使
用方法。4. The method for using a microorganism according to claim 2, wherein the drug-resistant mutation operation performed on the micromonospora is performed in a phenolic fungicide at a predetermined concentration for 30 minutes or more.
％水溶液であることを特徴とする請求項４記載の微生物
の使用方法。5. The phenolic bactericide is cresol-1.
The method for using a microorganism according to claim 4, wherein the microorganism is a 0.1% aqueous solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11121294A JP2000308483A (en) | 1999-04-28 | 1999-04-28 | Use of microorganism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11121294A JP2000308483A (en) | 1999-04-28 | 1999-04-28 | Use of microorganism |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2000308483A true JP2000308483A (en) | 2000-11-07 |
Family
ID=14807706
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11121294A Withdrawn JP2000308483A (en) | 1999-04-28 | 1999-04-28 | Use of microorganism |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2000308483A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104172353A (en) * | 2014-06-16 | 2014-12-03 | 四川溪源水产养殖有限公司 | Giant salamander polypeptide beverage and preparation method thereof |
-
1999
- 1999-04-28 JP JP11121294A patent/JP2000308483A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104172353A (en) * | 2014-06-16 | 2014-12-03 | 四川溪源水产养殖有限公司 | Giant salamander polypeptide beverage and preparation method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A300 | Withdrawal of application because of no request for examination |
Free format text: JAPANESE INTERMEDIATE CODE: A300 Effective date: 20060704 |