CN102697042A - Method for extracting amino acid nutrient solution from meat of Ostrea rivularis and product - Google Patents
Method for extracting amino acid nutrient solution from meat of Ostrea rivularis and product Download PDFInfo
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- CN102697042A CN102697042A CN2012101865870A CN201210186587A CN102697042A CN 102697042 A CN102697042 A CN 102697042A CN 2012101865870 A CN2012101865870 A CN 2012101865870A CN 201210186587 A CN201210186587 A CN 201210186587A CN 102697042 A CN102697042 A CN 102697042A
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Abstract
The invention discloses a method for extracting amino acid nutrient solution from meat of Ostrea rivularis. The Ostrea rivularis is used as a main raw material and the amino acid nutrient solution is prepared through pretreatment, meat obtaining, soaking, homogenization, enzymatic hydrolysis, centrifugal separation, blending, filling, sterilization and packaging. By determining the optimum time of soaking for sand removal and the temporary ice water storage process and by using enzymes in a combined way, the yield of products is higher than the yield of the products obtained by a single enzymatic hydrolysis method in the prior art, and compared with the prior art, the method provided by the invention is simple to operate and easy to control.
Description
Technical field
The present invention relates to a kind of method of from the oyster meat of Crassostrea rivularis, extracting amino-acid nutrition liquid, and the product that adopts this method to obtain.
Background technology
Crassostrea rivularis has another name called big oyster, is one of Qiezhou, Guangxi four big famous and precious marine products, and meat can be eaten raw, also can be processed into dried oyster, oyster sauce.Big oyster oyster meat is the aquatic products with very high nutrition and health care function of high protein, low fat, delicious flavour, and oyster meat protein content surpasses 45%, and is nutritious, and delicious flavour have the title of " marine milk ", also can be used as medicine simultaneously.Big oyster meat is rich in taurine, can promote the human brain development.
In the west, oyster is called as " milk in sea ", " sea fruit ", and oyster meat was once thought " among the extra large family your person " in ancient times, and this has affirmed fully the nutritive value of oyster.Oyster contains rich in protein, amino acid, also contains abundant vitamin such as VA, VB
1, VB
2, VB
3, VB
12, VC, VD
2, VH etc., contain inorganic salts and trace element in the oyster meat, human body is had the important physical effect.
Along with improving constantly of living standard, the change of people's diet structure, the food of pursuit nutrition height and health care has become people people's fashion.Therefore, big oyster aquatic products receive consumers in general's welcome deeply.Containing 19 kinds is the amino acid of needed by human, and taurine is the peculiar composition of oyster sauce, and is rich in 20 various trace elements such as zinc, is desirable strong kidney tonic nutrient article and health food of the male sex, favored by consumers in general, and market prospects are huge.
Along with going from strength to strength of oyster culture industry, the oyster resource becomes increasingly abundant, and the oyster deep processing is the only way which must be passed that industry enlarges development.
Summary of the invention
The object of the present invention is to provide a kind of method of from the oyster meat of Crassostrea rivularis, extracting amino-acid nutrition liquid, is primary raw material with the Crassostrea rivularis, through pre-treatment, get meat, immersion, homogenate, enzymolysis, centrifugal, allotment, can, sterilization, packing is processed.
Another object of the present invention is to provide a kind of amino-acid nutrition liquid that adopts method for preparing to obtain.
The present invention includes following steps:
1), pre-treatment: choose freshness Crassostrea rivularis preferably,, place flowing seawater after filtering to Crassostrea rivularis, soak and tell sand with spraying seawer washing;
2), get meat: insert in the shell until closed flesh from the oyster shell trailing edge with blade, the blade that swings is cut from its closed flesh and is made it open shell; Cut from the closed flesh that is attached on another shell sheet according to this method, oyster meat just entirely breaks away from the oyster shell again, cuts and leaves from sticking to closed flesh top on the oyster shell in the operation, oyster meat is temporarily stored in the frozen water that has added trash ice;
3), soak: oyster meat is taken out from frozen water, be immersed in to contain in 1.5-2.0% sodium chloride, 0.2-0.3% polymeric phosphate and the 0.15% lemon aqueous acid and soak 15-20min;
4), homogenate: oyster meat after will soaking drops into washes clean in the clear water, clean oyster meat and pure water are pressed the mixing of 1:0.5-1.0 weight ratio after, smash homogenate to pieces with bruisher, the rotating speed of homogenate is 10000-12000 rev/min, the time is 8-15 minute;
5), enzymolysis: the slurries after the collection homogenate; Add enzyme and carry out enzymolysis; Used enzyme is selected from one or both among Alcalase, PTN 6.0S, Protamex, Neutrase and the Papain; The consumption of enzyme is 0.1-0.2g enzyme/100g slurries, and hydrolysis temperature is 45-55 ℃, and the time is 6-10 hour;
6), centrifugal: as the slurries behind the enzymolysis to be heated to 80 ℃, to let enzyme deactivation, use centrifuge centrifuging and taking supernatant then;
7), adjustment: collects the supernatant that obtains after centrifugal,, adjust crude protein weight content to 3.0% wherein, obtain amino-acid nutrition liquid through concentrating;
8), canned, pasteurization, pack finished product.
Oyster meat of the present invention is meant the software part of oyster, and promptly the smart ovum part of oyster does not comprise closed flesh.
It is 18-20 hour that the husky time is told in the immersion of pre-treatment of the present invention.
Of the present invention getting in the meat step, the temperature maintenance of frozen water are at 8-12 ℃, and the temporary time is 10-15 minute.
Tell husky time and the temporary technology of frozen water for definite best immersion, the inventor makes up a large amount of contrast work of having done to different processes, part process conditions and result is listed at present:
Enzymolysis of the present invention; Used enzyme is the mixture of Alcalase or Protamex and PTN 6.0S preferably; The consumption of enzyme is 0.1-0.2g enzyme/100g slurries, and wherein Alcalase or Protamex account for the 20-30% of the total consumption of enzyme, and PTN 6.0S accounts for the 70-80% of the total consumption of enzyme; Hydrolysis temperature is 45-55 ℃, and the time is 6-10 hour.
Of the present invention centrifugal, rotating speed is 4500-5000 rev/min, and the time is 8-15 minute.
Carry out the technological effect of enzymolysis for explanation the present invention adopts different enzymes, done following technology contrast, concrete experiment condition and result are following:
Can find out that from above-mentioned experimental data the said method of the application is used through combination collocation that enzyme is carried out, make the yield of product than the height of single enzyme solution gained yield in the prior art.
A kind of oyster amino acid energy nutrient solution adopts above-mentioned preparation method to obtain, and has following technical indicator: the requirement of table 1 sense organ
| Project | Requirement |
| Color and luster | Colourless to sundown, impurity and suspension. |
| Flavour and smell | Tasteless |
| Form | Clear liquid does not have deposition |
| Impurity | The exogenous impurity that no twenty-twenty vision is visible |
Table 2 physical and chemical index
Table 3 microbiological indicator
| Project | Index |
| Total number of bacteria | <=1OO/mL |
| Escherichia coli | <=3/mL |
| Pathogenic bacteria | Must not detect |
The invention has the advantages that:
1, the present invention is through the combination of temporary time of soak time, frozen water temperature and frozen water in the oyster process, and the factor that the fragmentation of oyster body, body fluid is outflowed, influences quality and oyster meat yield has been carried out better controlling, has obtained good effect;
2, the method for the invention is used through the combination collocation that enzyme is carried out; Make the yield of product than the height of single enzyme solution gained yield in the prior art; Compared with prior art, the said method of the application is simple to operate, easy to be controlled, and has improved the yield of product greatly;
3, the oyster amino acid energy nutrient solution that obtains of the present invention; Contain rich in amino acid, can be directly use, can be used as also that other compositions of health food combine in healthy food material and the prior art as health food; Adopt prior art, prepare various forms of health foods;
3, through utilizing deep processing, improve product surcharge and competitiveness, promoted this area The development in society and economy oyster.
The specific embodiment
The present invention will be described with embodiment below, but the present invention is not limited to these embodiment.
Embodiment 1
1), pre-treatment: choose freshness Crassostrea rivularis preferably,, place flowing seawater after filtering to Crassostrea rivularis, soak and tell husky 18 hours with spraying seawer washing;
2), get meat: insert in the shell until closed flesh from the oyster shell trailing edge with blade, the blade that swings is cut from its closed flesh and is made it open shell; Cut from the closed flesh that is attached on another shell sheet according to this method, oyster meat just entirely breaks away from the oyster shell again, cuts and leaves from sticking to closed flesh top on the oyster shell in the operation, oyster meat is temporarily stored in the frozen water that has added trash ice 8 ℃ of frozen water temperature, temporary 15 minutes time;
3), soak: oyster meat is taken out from frozen water, be immersed in to contain in 1.5% sodium chloride, 0.3% polymeric phosphate and the 0.15% lemon aqueous acid and soak 15min;
4), homogenate: the oyster meat after will soaking drops into washes clean in the clear water, after clean oyster meat 10kg and pure water 5kg mixing, smashs homogenate to pieces with high-speed tissue mashing machine, and the rotating speed of homogenate is 10000 rev/mins, and the time is 8 minutes;
5), enzymolysis: collect the slurries after the homogenate, add the Alcalase enzyme and carry out enzymolysis, the consumption of enzyme is 0.1g enzyme/100g slurries, and hydrolysis temperature is 45 ℃, and the time is 8 hours;
6), centrifugal: the slurries behind the enzymolysis are heated to 80 ℃, let enzyme deactivation, use centrifuge centrifuging and taking supernatant then, rotating speed is 4500 rev/mins, and the time is 15 minutes;
7), adjustment: collects the supernatant that obtains after centrifugal,, adjust crude protein weight content to 3.0% wherein, obtain amino-acid nutrition liquid 7.1kg through concentrating;
8), canned, pasteurization, pack finished product.
Embodiment 2
1), pre-treatment: choose freshness Crassostrea rivularis preferably,, place flowing seawater after filtering to Crassostrea rivularis, soak and tell husky 20 hours with spraying seawer washing;
2), get meat: insert in the shell until closed flesh from the oyster shell trailing edge with blade, the blade that swings is cut from its closed flesh and is made it open shell; Cut from the closed flesh that is attached on another shell sheet according to this method, oyster meat just entirely breaks away from the oyster shell again, cuts and leaves from sticking to closed flesh top on the oyster shell in the operation, oyster meat is temporarily stored in the frozen water that has added trash ice 12 ℃ of frozen water temperature, temporary 10 minutes time;
3), soak: oyster meat is taken out from frozen water, be immersed in to contain in 2.0% sodium chloride, 0.2% polymeric phosphate and the 0.15% lemon aqueous acid and soak 20min;
4), homogenate: the oyster meat after will soaking drops into washes clean in the clear water, after clean oyster meat 10kg and pure water 10kg mixing, smashs homogenate to pieces with high-speed tissue mashing machine, and the rotating speed of homogenate is 12000 rev/mins, and the time is 15 minutes;
5), enzymolysis: collect the slurries after the homogenate, add PTN 6.0S enzyme and carry out enzymolysis, the consumption of enzyme is 0.2g enzyme/100g slurries, and hydrolysis temperature is 55 ℃, and the time is 6 hours;
6), centrifugal: the slurries behind the enzymolysis are heated to 80 ℃, let enzyme deactivation, use centrifuge centrifuging and taking supernatant then, rotating speed is 5000 rev/mins, and the time is 8 minutes;
7), adjustment: collects the supernatant that obtains after centrifugal,, adjust crude protein weight content to 3.0% wherein, obtain amino-acid nutrition liquid 7.3kg through concentrating;
8), canned, pasteurization, pack finished product.
Embodiment 3
1), pre-treatment: choose freshness Crassostrea rivularis preferably,, place flowing seawater after filtering to Crassostrea rivularis, soak and tell husky 18 hours with spraying seawer washing;
2), get meat: insert in the shell until closed flesh from the oyster shell trailing edge with blade, the blade that swings is cut from its closed flesh and is made it open shell; Cut from the closed flesh that is attached on another shell sheet according to this method, oyster meat just entirely breaks away from the oyster shell again, cuts and leaves from sticking to closed flesh top on the oyster shell in the operation, oyster meat is temporarily stored in the frozen water that has added trash ice 10 ℃ of frozen water temperature, temporary 15 minutes time;
3), soak: oyster meat is taken out from frozen water, be immersed in to contain in 1.5% sodium chloride, 0.3% polymeric phosphate and the 0.15% lemon aqueous acid and soak 15min;
4), homogenate: the oyster meat after will soaking drops into washes clean in the clear water, after clean oyster meat 10kg and pure water 8kg mixing, smashs homogenate to pieces with high-speed tissue mashing machine, and the rotating speed of homogenate is 10000 rev/mins, and the time is 8 minutes;
5), enzymolysis: collect the slurries after the homogenate, add the Protamex enzyme and carry out enzymolysis, the consumption of enzyme is 0.1g enzyme/100g slurries, and hydrolysis temperature is 45 ℃, and the time is 10 hours;
6), centrifugal: the slurries behind the enzymolysis are heated to 80 ℃, let enzyme deactivation, use centrifuge centrifuging and taking supernatant then, rotating speed is 4500 rev/mins, and the time is 15 minutes;
7), adjustment: collects the supernatant that obtains after centrifugal,, adjust crude protein weight content to 3.0% wherein, obtain amino-acid nutrition liquid 6.6kg through concentrating;
8), canned, pasteurization, pack finished product.
Embodiment 4
1), pre-treatment: choose freshness Crassostrea rivularis preferably,, place flowing seawater after filtering to Crassostrea rivularis, soak and tell husky 20 hours with spraying seawer washing;
2), get meat: insert in the shell until closed flesh from the oyster shell trailing edge with blade, the blade that swings is cut from its closed flesh and is made it open shell; Cut from the closed flesh that is attached on another shell sheet according to this method, oyster meat just entirely breaks away from the oyster shell again, cuts and leaves from sticking to closed flesh top on the oyster shell in the operation, oyster meat is temporarily stored in the frozen water that has added trash ice 8 ℃ of frozen water temperature, temporary 12 minutes time;
3), soak: oyster meat is taken out from frozen water, be immersed in to contain in 2.0% sodium chloride, 0.2% polymeric phosphate and the 0.15% lemon aqueous acid and soak 20min;
4), homogenate: the oyster meat after will soaking drops into washes clean in the clear water, after clean oyster meat 10kg and pure water 6kg mixing, smashs homogenate to pieces with high-speed tissue mashing machine, and the rotating speed of homogenate is 12000 rev/mins, and the time is 15 minutes;
5), enzymolysis: collect the slurries after the homogenate, add the Neutrase enzyme and carry out enzymolysis, the consumption of enzyme is 0.2g enzyme/100g slurries, and hydrolysis temperature is 55 ℃, and the time is 6 hours;
6), centrifugal: the slurries behind the enzymolysis are heated to 80 ℃, let enzyme deactivation, use centrifuge centrifuging and taking supernatant then, rotating speed is 5000 rev/mins, and the time is 8 minutes;
7), adjustment: collects the supernatant that obtains after centrifugal,, adjust crude protein weight content to 3.0% wherein, obtain amino-acid nutrition liquid 6.5kg through concentrating;
8), canned, pasteurization, pack finished product.
Embodiment 5
1), pre-treatment: choose freshness Crassostrea rivularis preferably,, place flowing seawater after filtering to Crassostrea rivularis, soak and tell husky 18 hours with spraying seawer washing;
2), get meat: insert in the shell until closed flesh from the oyster shell trailing edge with blade, the blade that swings is cut from its closed flesh and is made it open shell; Cut from the closed flesh that is attached on another shell sheet according to this method, oyster meat just entirely breaks away from the oyster shell again, cuts and leaves from sticking to closed flesh top on the oyster shell in the operation, oyster meat is temporarily stored in the frozen water that has added trash ice 12 ℃ of frozen water temperature, temporary 10 minutes time;
3), soak: oyster meat is taken out from frozen water, be immersed in to contain in 1.5% sodium chloride, 0.3% polymeric phosphate and the 0.15% lemon aqueous acid and soak 15min;
4), homogenate: the oyster meat after will soaking drops into washes clean in the clear water, after clean oyster meat 10kg and pure water 5kg mixing, smashs homogenate to pieces with high-speed tissue mashing machine, and the rotating speed of homogenate is 10000 rev/mins, and the time is 8 minutes;
5), enzymolysis: collect the slurries after the homogenate, add the Papain enzyme and carry out enzymolysis, the consumption of enzyme is 0.1g enzyme/100g slurries, and hydrolysis temperature is 50 ℃, and the time is 10 hours;
6), centrifugal: the slurries behind the enzymolysis are heated to 80 ℃, let enzyme deactivation, use centrifuge centrifuging and taking supernatant then, rotating speed is 4500 rev/mins, and the time is 15 minutes;
7), adjustment: collects the supernatant that obtains after centrifugal,, adjust crude protein weight content to 3.0% wherein, obtain amino-acid nutrition liquid 6.1kg through concentrating;
8), canned, pasteurization, pack finished product.
Embodiment 6
1), pre-treatment: choose freshness Crassostrea rivularis preferably,, place flowing seawater after filtering to Crassostrea rivularis, soak and tell husky 20 hours with spraying seawer washing;
2), get meat: insert in the shell until closed flesh from the oyster shell trailing edge with blade, the blade that swings is cut from its closed flesh and is made it open shell; Cut from the closed flesh that is attached on another shell sheet according to this method, oyster meat just entirely breaks away from the oyster shell again, cuts and leaves from sticking to closed flesh top on the oyster shell in the operation, oyster meat is temporarily stored in the frozen water that has added trash ice 10 ℃ of frozen water temperature, temporary 15 minutes time;
3), soak: oyster meat is taken out from frozen water, be immersed in to contain in 2.0% sodium chloride, 0.2% polymeric phosphate and the 0.15% lemon aqueous acid and soak 20min;
4), homogenate: the oyster meat after will soaking drops into washes clean in the clear water, after clean oyster meat 10kg and pure water 10kg mixing, smashs homogenate to pieces with high-speed tissue mashing machine, and the rotating speed of homogenate is 12000 rev/mins, and the time is 10 minutes;
5), enzymolysis: the slurries after the collection homogenate; The mixture that adds Alcalase and PTN 6.0S enzyme, wherein Alcalase and PTN 6.0S account for 20% and 80% of the total consumption of enzyme respectively and carry out enzymolysis, and the consumption of enzyme is 0.15g enzyme/100g slurries; Hydrolysis temperature is 50 ℃, and the time is 8 hours;
6), centrifugal: the slurries behind the enzymolysis are heated to 80 ℃, let enzyme deactivation, use centrifuge centrifuging and taking supernatant then, rotating speed is 5000 rev/mins, and the time is 8 minutes;
7), adjustment: collects the supernatant that obtains after centrifugal,, adjust crude protein weight content to 3.0% wherein, obtain amino-acid nutrition liquid 8.5kg through concentrating;
8), canned, pasteurization, pack finished product.
Embodiment 7
1), pre-treatment: choose freshness Crassostrea rivularis preferably,, place flowing seawater after filtering to Crassostrea rivularis, soak and tell husky 18 hours with spraying seawer washing;
2), get meat: insert in the shell until closed flesh from the oyster shell trailing edge with blade, the blade that swings is cut from its closed flesh and is made it open shell; Cut from the closed flesh that is attached on another shell sheet according to this method, oyster meat just entirely breaks away from the oyster shell again, cuts and leaves from sticking to closed flesh top on the oyster shell in the operation, oyster meat is temporarily stored in the frozen water that has added trash ice 8 ℃ of frozen water temperature, temporary 12 minutes time;
3), soak: oyster meat is taken out from frozen water, be immersed in to contain in 1.5% sodium chloride, 0.3% polymeric phosphate and the 0.15% lemon aqueous acid and soak 15min;
4), homogenate: the oyster meat after will soaking drops into washes clean in the clear water, after clean oyster meat 10kg and pure water 8kg mixing, smashs homogenate to pieces with high-speed tissue mashing machine, and the rotating speed of homogenate is 10000 rev/mins, and the time is 12 minutes;
5), enzymolysis: the slurries after the collection homogenate; The mixture that adds Alcalase and PTN 6.0S enzyme, wherein Alcalase and PTN 6.0S account for 30% and 70% of the total consumption of enzyme respectively and carry out enzymolysis, and the consumption of enzyme is 0.20g enzyme/100g slurries; Hydrolysis temperature is 45 ℃, and the time is 10 hours;
6), centrifugal: the slurries behind the enzymolysis are heated to 80 ℃, let enzyme deactivation, use centrifuge centrifuging and taking supernatant then, rotating speed is 4500 rev/mins, and the time is 15 minutes;
7), adjustment: collects the supernatant that obtains after centrifugal,, adjust crude protein weight content to 3.0% wherein, obtain amino-acid nutrition liquid 8.6kg through concentrating;
8), canned, pasteurization, pack finished product.
Embodiment 8
1), pre-treatment: choose freshness Crassostrea rivularis preferably,, place flowing seawater after filtering to Crassostrea rivularis, soak and tell husky 20 hours with spraying seawer washing;
2), get meat: insert in the shell until closed flesh from the oyster shell trailing edge with blade, the blade that swings is cut from its closed flesh and is made it open shell; Cut from the closed flesh that is attached on another shell sheet according to this method, oyster meat just entirely breaks away from the oyster shell again, cuts and leaves from sticking to closed flesh top on the oyster shell in the operation, oyster meat is temporarily stored in the frozen water that has added trash ice 12 ℃ of frozen water temperature, temporary 10 minutes time;
3), soak: oyster meat is taken out from frozen water, be immersed in to contain in 2.0% sodium chloride, 0.2% polymeric phosphate and the 0.15% lemon aqueous acid and soak 20min;
4), homogenate: the oyster meat after will soaking drops into washes clean in the clear water, after clean oyster meat 10kg and pure water 6kg mixing, smashs homogenate to pieces with high-speed tissue mashing machine, and the rotating speed of homogenate is 12000 rev/mins, and the time is 8 minutes;
5), enzymolysis: the slurries after the collection homogenate; The mixture that adds Protamex and PTN 6.0S enzyme, wherein Protamex and PTN 6.0S account for 20% and 80% of the total consumption of enzyme respectively and carry out enzymolysis, and the consumption of enzyme is 0.15g enzyme/100g slurries; Hydrolysis temperature is 50 ℃, and the time is 8 hours;
6), centrifugal: the slurries behind the enzymolysis are heated to 80 ℃, let enzyme deactivation, use centrifuge centrifuging and taking supernatant then, rotating speed is 5000 rev/mins, and the time is 8 minutes;
7), adjustment: collects the supernatant that obtains after centrifugal,, adjust crude protein weight content to 3.0% wherein, obtain amino-acid nutrition liquid 8.7kg through concentrating;
8), canned, pasteurization, pack finished product.
Embodiment 9
1), pre-treatment: choose freshness Crassostrea rivularis preferably,, place flowing seawater after filtering to Crassostrea rivularis, soak and tell husky 18 hours with spraying seawer washing;
2), get meat: insert in the shell until closed flesh from the oyster shell trailing edge with blade, the blade that swings is cut from its closed flesh and is made it open shell; Cut from the closed flesh that is attached on another shell sheet according to this method, oyster meat just entirely breaks away from the oyster shell again, cuts and leaves from sticking to closed flesh top on the oyster shell in the operation, oyster meat is temporarily stored in the frozen water that has added trash ice 8 ℃ of frozen water temperature, temporary 15 minutes time;
3), soak: oyster meat is taken out from frozen water, be immersed in to contain in 1.5% sodium chloride, 0.3% polymeric phosphate and the 0.15% lemon aqueous acid and soak 15min;
4), homogenate: the oyster meat after will soaking drops into washes clean in the clear water, after clean oyster meat 10kg and pure water 5kg mixing, smashs homogenate to pieces with high-speed tissue mashing machine, and the rotating speed of homogenate is 10000 rev/mins, and the time is 12 minutes;
5), enzymolysis: the slurries after the collection homogenate; The mixture that adds Protamex and PTN 6.0S enzyme, wherein Protamex and PTN 6.0S account for 30% and 70% of the total consumption of enzyme respectively and carry out enzymolysis, and the consumption of enzyme is 0.20g enzyme/100g slurries; Hydrolysis temperature is 45 ℃, and the time is 10 hours;
6), centrifugal: the slurries behind the enzymolysis are heated to 80 ℃, let enzyme deactivation, use centrifuge centrifuging and taking supernatant then, rotating speed is 4500 rev/mins, and the time is 15 minutes;
7), adjustment: collects the supernatant that obtains after centrifugal,, adjust crude protein weight content to 3.0% wherein, obtain amino-acid nutrition liquid 8.3kg through concentrating;
8), canned, pasteurization, pack finished product.
Embodiment 10
The product that adopts embodiment 1-9 to obtain has following technical indicator:
The requirement of table 1 sense organ
| Project | Requirement |
| Color and luster | Colourless to sundown, impurity and suspension. |
| Flavour and smell | Tasteless |
| Form | Clear liquid does not have deposition |
| Impurity | The exogenous impurity that no twenty-twenty vision is visible |
Table 2 physical and chemical index
Table 3 microbiological indicator
| Project | Index |
| Total number of bacteria | 1OO/mL |
| Escherichia coli | <=3/mL |
| Pathogenic bacteria | Must not detect |
Claims (7)
1. from the oyster meat of Crassostrea rivularis, extract the method for amino-acid nutrition liquid, comprise the steps:
1), pre-treatment: choose freshness Crassostrea rivularis preferably,, place flowing seawater after filtering to Crassostrea rivularis, soak and tell sand with spraying seawer washing;
2), get meat: insert in the shell until closed flesh from the oyster shell trailing edge with blade, the blade that swings is cut from its closed flesh and is made it open shell; Cut from the closed flesh that is attached on another shell sheet according to this method, oyster meat just entirely breaks away from the oyster shell again, cuts and leaves from sticking to closed flesh top on the oyster shell in the operation, oyster meat is temporarily stored in the frozen water that has added trash ice;
3), soak: oyster meat is taken out from frozen water, be immersed in to contain in 1.5-2.0% sodium chloride, 0.2-0.3% polymeric phosphate and the 0.15% lemon aqueous acid and soak 15-20min;
4), homogenate: oyster meat after will soaking drops into washes clean in the clear water, clean oyster meat and pure water are pressed the mixing of 1:0.5-1.0 weight ratio after, smash homogenate to pieces with bruisher, the rotating speed of homogenate is 10000-12000 rev/min, the time is 8-15 minute;
5), enzymolysis: the slurries after the collection homogenate; Add enzyme and carry out enzymolysis; Used enzyme is selected from one or both among Alcalase, PTN 6.0S, Protamex, Neutrase and the Papain; The consumption of enzyme is 0.1-0.2g enzyme/100g slurries, and hydrolysis temperature is 45-55 ℃, and the time is 6-10 hour;
6), centrifugal: as the slurries behind the enzymolysis to be heated to 80 ℃, to let enzyme deactivation, use centrifuge centrifuging and taking supernatant then;
7), adjustment: collects the supernatant that obtains after centrifugal,, adjust crude protein weight content to 3.0% wherein, obtain amino-acid nutrition liquid through concentrating;
8), canned, pasteurization, pack finished product.
2. the method for from the oyster meat of Crassostrea rivularis, extracting amino-acid nutrition liquid according to claim 1, it is characterized in that: oyster meat is meant the software part of oyster, promptly the smart ovum part of oyster does not comprise closed flesh.
3. the method for from the oyster meat of Crassostrea rivularis, extracting amino-acid nutrition liquid according to claim 1 is characterized in that: it is 18-20 hour that the husky time is told in the immersion of described pre-treatment.
4. the method for from the oyster meat of Crassostrea rivularis, extracting amino-acid nutrition liquid according to claim 1 is characterized in that: described getting in the meat step, the temperature maintenance of frozen water are at 8-12 ℃, and the temporary time is 10-15 minute.
5. the method for from the pearl foetal sac of mother pearl shellfish, extracting pearl foetal sac liquid according to claim 1; It is characterized in that: described enzymolysis, used enzyme are the mixture of Alcalase or Protamex and PTN 6.0S, and the consumption of enzyme is 0.1-0.2g enzyme/100g slurries; Wherein Alcalase or Protamex account for the 20-30% of the total consumption of enzyme; PTN 6.0S accounts for the 70-80% of the total consumption of enzyme, and hydrolysis temperature is 45-55 ℃, and the time is 6-10 hour.
6. the method for from the pearl foetal sac of mother pearl shellfish, extracting pearl foetal sac liquid according to claim 1, it is characterized in that: described centrifugal, rotating speed is 4500-5000 rev/min, and the time is 8-15 minute.
7. the oyster amino acid energy nutrient solution that each said method makes in the claim 1～7.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104000243A (en) * | 2014-06-13 | 2014-08-27 | 福建省水产研究所 | Method for extracting oyster powder from oyster cooking liquor |
| CN104000243B (en) * | 2014-06-13 | 2016-07-06 | 福建省水产研究所 | A kind of method extracting Concha Ostreae powder from Concha Ostreae cooking liquor |
| CN104382034A (en) * | 2014-12-05 | 2015-03-04 | 北海富安源生物科技有限公司 | Nutritional oral solution produced by taking oysters as raw materials and having functions of dispelling effects of alcohol, protecting liver and improving functions |
| CN104687058A (en) * | 2015-03-31 | 2015-06-10 | 广西还珠海洋生物科技有限公司 | Ostrea rivularis nutrient solution and preparation method thereof |
| CN104687057A (en) * | 2015-03-31 | 2015-06-10 | 广西还珠海洋生物科技有限公司 | Small reef oyster de-alcoholic medicine and preparation method thereof |
| CN106262015A (en) * | 2016-08-16 | 2017-01-04 | 广西还珠海洋生物科技有限公司 | A kind of sea reef little oyster oyster liquid and preparation method thereof |
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