CN107998369A - A kind of preparation method of the oligomeric peptides products of ox bone collagen albumen - Google Patents
A kind of preparation method of the oligomeric peptides products of ox bone collagen albumen Download PDFInfo
- Publication number
- CN107998369A CN107998369A CN201711324823.XA CN201711324823A CN107998369A CN 107998369 A CN107998369 A CN 107998369A CN 201711324823 A CN201711324823 A CN 201711324823A CN 107998369 A CN107998369 A CN 107998369A
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- China
- Prior art keywords
- bone
- weight
- ratio
- collagen albumen
- bone collagen
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Abstract
An embodiment of the present invention provides a kind of preparation method of the oligomeric peptides products of ox bone collagen albumen, include the following steps：Choose the ox bone after fresh clean to be put into liquid nitrogen and cool down, solidliquid mixture is obtained with after crusher in crushing, adding after extraction solution is extracted after taking-up；Mixed enzyme is added into solidliquid mixture, obtains the first enzymolysis liquid；Protein incision enzyme is added into the first enzymolysis liquid, obtains the second enzymolysis liquid；Chinese medical extract is added into the second enzymolysis liquid, is stirred continuously and is warming up to boiling, mixed extract is obtained after centrifugal filtration；After mixed extract is separated, concentrated and is dried, the oligomeric peptides products of ox bone collagen albumen are obtained.The oligomeric peptides products of ox bone collagen albumen prepared by this method have zhuanggu, increase bone density, promote bone growth healing, treatment of arthritis and other effects, the Chinese medicinal component of addition can be cooperateed with ox bone collagen albumen oligopeptide, easily absorb, further enhancing zhuanggu strong bone, treat and prevent the effect of osteopathy.
Description
【Technical field】
The present invention relates to personal care product's technical field, more particularly to a kind of system of the oligomeric peptides products of ox bone collagen albumen
Preparation Method.
【Background technology】
With advancing age, the bone density of human body can be more and more lower, for the elderly, usually occurs
The orthopaedic disease as caused by bone density loss such as osteoporosis, for this kind of crowd, it is necessary to which the high zhuanggu of edible nutritional value is mended
Product build up health, improve the quality of living to improve human body itself bone density.
Main in ox bone to contain collagen, sweet in flavor, warm-natured, the thoughts of returning home, kidney, large intestine channel, have and exempt from numbness, preventing malaria, the work(of sore
Effect, therefore it is usually used in arthritis, rush down dysentery, malaria, infantile malnutrition due to digestive disturbances or intestinalparasites sore.Therefore, one of zhuanggu tonic high as nutritive value is often by people
Favor.Ox bone usually enters human body in a manner of edible or decoction, and it is oligomeric that its collagen, which will generally be digested liquid enzymolysis,
Peptide or amino acid and be absorbed by organisms and utilize, and older population is common weakness of the spleen and the stomach or has gastrointestinal reaction, therefore alimentary canal
Active low, the digestible energy force difference of enzyme, thus it is above-mentioned take the burden for not only resulting in people digest's system, and do not reach
Due drug effect.
It is the mankind and ox bone collagen albumen is obtained small molecule donkey-hide gelatin peptide by processing can effectively overcome the above problem
Health brings glad tidings.Therefore, the high oligomeric peptides products of ox bone collagen albumen of a kind of suitable industrialized production, product quality are invented
Preparation method be those skilled in the art's technical problem urgently to be resolved hurrily.
【The content of the invention】
In view of this, an embodiment of the present invention provides a kind of preparation method of the oligomeric peptides products of ox bone collagen albumen.
A kind of preparation method of the oligomeric peptides products of ox bone collagen albumen, includes the following steps：
1) choose it is fresh clean after ox bone masking foil it is fully wrapped around after, be then placed in liquid nitrogen and cool down 1min, take out
Ox bone is crushed to 100 mesh with crusher afterwards, adds after extraction solution is extracted and obtains solidliquid mixture；Wherein, it is described to carry
It is the mixed solution that solvent is water to take solution, and the concentration of each solute is respectively：Sodium hydroxide 100mM, sodium chloride 50mM, sucrose
10mM, extraction process carry out in the microwave environment of 400W；
2) mixed enzyme is added into solidliquid mixture, it is 6 to add newborn acid for adjusting pH value, the enzyme in 38 DEG C of isoperibol
60min is solved, obtains the first enzymolysis liquid, wherein mixed enzyme is that ratio of weight and number is 4:1:5:2 Collagenase, lipase, sugar
First enzyme and nuclease mix enzyme system；Keep temperature and pH value constant, the parts by weight such as addition and mixed enzyme into the first enzymolysis liquid
Protein incision enzyme, digest 90min after obtain the second enzymolysis liquid；
3) added into the second enzymolysis liquid and ox bone ratio of weight and number is 1:150 Chinese medical extract, is stirred continuously heating
To boiling, and 20min is kept, be then down to room temperature, mixed extract is obtained after centrifugal filtration；Wherein, Chinese medical extract is logical
Following steps are crossed to be prepared：
A, dry Chinese medicine composition is taken to be crushed, wherein it is 5 that the Chinese medicine composition, which is the ratio between parts by weight,:2:10:
4:3:1 keel, pig label grass, hoveniae semoveniae semen, chorion ovi, fenugreek and bitter orange flower, then adds the ethanol that volume fraction is 90%
Aqueous solution refluxing extraction 5 times, adds ethanol water as 2 times of weight of dry Chinese medicine composition, return time 4h every time；Receive
Collect and merge extracting solution, be concentrated under reduced pressure into no alcohol, obtain thick medicinal extract；
B, the thick medicinal extract obtained by step a is suspended in the water of dry 2 times of volumes of Chinese medicine composition, then uses petroleum ether
Extraction 3 times, the amount for adding petroleum ether every time is 0.3 times of volume of thick medicinal extract, and organic phase is merged after extraction, dry, decompression
Concentration, obtains Chinese medicine crude extract；
C. the Chinese medicine crude extract obtained by step b is dissolved in methyl butyrate with the concentration of 1.0g/ml, upper silica gel column chromatography
Column；
D. gradient elution：Elution flow is 6ml/min, first elutes 5 column volumes with petroleum ether, removes flow point；Body is used again
Product is than being 3:1 petroleum ether and dichloromethane mixed liquor elutes 6 column volumes, collects flow point；It is again 5 with volume ratio:3:1
The mixed liquor of petroleum ether, dichloromethane and methyl butyrate elutes 8 column volumes, removes flow point；It is again 4 with volume ratio:3:2
The mixed liquor of petroleum ether, dichloromethane and methyl butyrate elutes 20 column volumes, collects flow point, and the flow point of collection is merged, is subtracted
Pressure concentration, up to Chinese medical extract；
4) after mixed extract is separated, concentrated and dried, the oligomeric peptides products of ox bone collagen albumen are obtained.
Present invention also offers stomach made of a kind of oligomeric peptides products of ox bone collagen albumen and pharmaceutically acceptable auxiliary material
Floating tablet, further includes auxiliary material, and the ratio of weight and number of the oligomeric peptides products of the ox bone collagen albumen and auxiliary material is 1:6, the auxiliary material
Include the component of following parts by weight：20 parts of hydrophilic gel, 30 parts of filler, 2 parts of cosolvent, 4 parts of foaming agent, lubricant 2
2 parts of part and foam stabilizer.
Preferably, it is 1 that the hydrophilic gel, which includes ratio of weight and number,:3:2.5 bean gum, guar gum and sieve
Shop sign in the form of a streamer glue.
Preferably, it is 1 that the filler, which includes ratio of weight and number,:3:4 crospovidone, vanillic aldehyde and wood
Sugar alcohol.
Preferably, it is 1 that the cosolvent, which includes ratio of weight and number,:2:3 polyvinylpyrrolidones, heparin sodium and bright
Propylhomoserin.
Preferably, it is 1 that the foaming agent, which includes ratio of weight and number,:4:2:3 citric acid fatty glyceride, alkane
Base glycine betaine, polyethylene glycol monolaurate and diethanolamine of fat acyl.
Preferably, it is 2 that the lubricant, which includes ratio of weight and number,:3:5 polyalphaolefin, magnesium laurylsulfate and mountain
Yu acid glyceryl esters.
Preferably, it is 1 that the foam stabilizer, which includes ratio of weight and number,:3:2 polyethylene glycol, saponin and methylol
Cellulose.
A technical solution in above-mentioned technical proposal has the advantages that：
The present invention provides a kind of preparation method of the oligomeric peptides products of ox bone collagen albumen, technique is advanced, process is reasonable,
It is workable, it is easy to industrialized production, the oligomeric peptides products of ox bone collagen albumen prepared by this method have zhuanggu, increase bone
Density, promote bone growth healing, treatment of arthritis and other effects, and the Chinese medicinal component of addition can be oligomeric with ox bone collagen albumen
Peptide is cooperateed with, it is easier to is absorbed, be further enhancing zhuanggu strong bone, treats and prevents the effect of osteopathy, in addition, in enzymolysis
The combination of enzyme and the selection of dosage so that enzymolysis efficiency and Degree of Enzymatic Hydrolysis are high, and in zymolyte amino chemicals species sum number
Amount is more, in good taste.
【Embodiment】
For a better understanding of the technical solution of the present invention, the embodiment of the present invention is described in detail below.
It will be appreciated that described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.
Based on the embodiments of the present invention, the institute that those of ordinary skill in the art are obtained without creative efforts
There are other embodiments, belong to the scope of protection of the invention.
The term used in embodiments of the present invention is only merely for the purpose of description specific embodiment, and is not intended to limit
The system present invention.The embodiment of the present invention and " one kind " of singulative used in the attached claims, " described " and
"the" is also intended to including most forms, unless context clearly shows that other implications.
It should be appreciated that term "and/or" used herein is only a kind of incidence relation for describing affiliated partner, table
Show there may be three kinds of relations, for example, A and/or B, can represent：Individualism A, while there are A and B, individualism B this
Three kinds of situations.In addition, character "/" herein, it is a kind of relation of "or" to typically represent forward-backward correlation object.
Embodiment 1
A kind of preparation method of the oligomeric peptides products of ox bone collagen albumen, includes the following steps：
1) choose it is fresh clean after ox bone masking foil it is fully wrapped around after, be then placed in liquid nitrogen and cool down 1min, take out
Ox bone is crushed to 100 mesh with crusher afterwards, adds after extraction solution is extracted and obtains solidliquid mixture；Wherein, it is described to carry
It is the mixed solution that solvent is water to take solution, and the concentration of each solute is respectively：Sodium hydroxide 100mM, sodium chloride 50mM, sucrose
10mM, extraction process carry out in the microwave environment of 400W；
2) mixed enzyme is added into solidliquid mixture, it is 6 to add newborn acid for adjusting pH value, the enzyme in 38 DEG C of isoperibol
60min is solved, obtains the first enzymolysis liquid, wherein mixed enzyme is that ratio of weight and number is 4:1:5:2 Collagenase, lipase, sugar
First enzyme and nuclease mix enzyme system；Keep temperature and pH value constant, the parts by weight such as addition and mixed enzyme into the first enzymolysis liquid
Protein incision enzyme, digest 90min after obtain the second enzymolysis liquid；
3) added into the second enzymolysis liquid and ox bone ratio of weight and number is 1:150 Chinese medical extract, is stirred continuously heating
To boiling, and 20min is kept, be then down to room temperature, mixed extract is obtained after centrifugal filtration；Wherein, Chinese medical extract is logical
Following steps are crossed to be prepared：
A, dry Chinese medicine composition is taken to be crushed, wherein it is 5 that the Chinese medicine composition, which is the ratio between parts by weight,:2:10:
4:3:1 keel, pig label grass, hoveniae semoveniae semen, chorion ovi, fenugreek and bitter orange flower, then adds the ethanol that volume fraction is 90%
Aqueous solution refluxing extraction 5 times, adds ethanol water as 2 times of weight of dry Chinese medicine composition, return time 4h every time；Receive
Collect and merge extracting solution, be concentrated under reduced pressure into no alcohol, obtain thick medicinal extract；
B, the thick medicinal extract obtained by step a is suspended in the water of dry 2 times of volumes of Chinese medicine composition, then uses petroleum ether
Extraction 3 times, the amount for adding petroleum ether every time is 0.3 times of volume of thick medicinal extract, and organic phase is merged after extraction, dry, decompression
Concentration, obtains Chinese medicine crude extract；
C. the Chinese medicine crude extract obtained by step b is dissolved in methyl butyrate with the concentration of 1.0g/ml, upper silica gel column chromatography
Column；
D. gradient elution：Elution flow is 6ml/min, first elutes 5 column volumes with petroleum ether, removes flow point；Body is used again
Product is than being 3:1 petroleum ether and dichloromethane mixed liquor elutes 6 column volumes, collects flow point；It is again 5 with volume ratio:3:1
The mixed liquor of petroleum ether, dichloromethane and methyl butyrate elutes 8 column volumes, removes flow point；It is again 4 with volume ratio:3:2
The mixed liquor of petroleum ether, dichloromethane and methyl butyrate elutes 20 column volumes, collects flow point, and the flow point of collection is merged, is subtracted
Pressure concentration, up to Chinese medical extract；
4) after mixed extract is separated, concentrated and dried, the oligomeric peptides products of ox bone collagen albumen are obtained.
Embodiment 2
The present embodiment is additionally provided made of a kind of oligomeric peptides products of ox bone collagen albumen and pharmaceutically acceptable auxiliary material
Gastric floating tablet, the gastric floating tablet further include auxiliary material, and the oligomeric peptides products of ox bone collagen albumen are 10g wherein in gastric floating tablet, auxiliary material
For 60g, auxiliary material includes following component：Hydrophilic gel 20g, filler 30g, cosolvent 2g, foaming agent 4g, lubricant 2g and
Foam stabilizer 2g.
Embodiment 3
The present embodiment is additionally provided made of a kind of oligomeric peptides products of ox bone collagen albumen and pharmaceutically acceptable auxiliary material
Gastric floating tablet, the gastric floating tablet further include auxiliary material, and the oligomeric peptides products of ox bone collagen albumen are 10g wherein in gastric floating tablet, auxiliary material
For 60g, auxiliary material includes following component：Ratio of weight and number is 1:3:The mixing parent of 2.5 bean gum, guar gum and tamarind gum
Aqueous gel 20g, ratio of weight and number 1:3:The mixed filler 30g of 4 crospovidone, vanillic aldehyde and xylose, weight
Portion rate is 1:2:The mixing cosolvent 2g of 3 polyvinylpyrrolidones, heparin sodium and leucine, ratio of weight and number 1:4:2:3
Citric acid fatty glyceride, alkyl betaine, polyethylene glycol monolaurate and diethanolamine of fat acyl mixing blistering
Agent 4g, ratio of weight and number 2:3:The mixed lubrication agent 2g of 5 polyalphaolefin, magnesium laurylsulfate and behenic acid glyceryl ester,
Ratio of weight and number is 1:3:The mixing foam stabilizer 2g of 2 polyethylene glycol, saponin and hydroxymethyl cellulose.
Reference examples 1
Reference examples 1 provide a kind of oligomeric peptides products of ox bone collagen albumen and stomach made of pharmaceutically acceptable auxiliary material
Floating tablet, the hydrophilic gel in the gastric floating tablet auxiliary material is that ratio of weight and number is 1:1:1 bean gum, guar gum and Luo Wang
The mixture of sub- glue, other are consistent with embodiment 3.
Reference examples 2
Reference examples 2 provide a kind of oligomeric peptides products of ox bone collagen albumen and stomach made of pharmaceutically acceptable auxiliary material
Floating tablet, the hydrophilic gel in the gastric floating tablet auxiliary material is peach gum, other are consistent with embodiment 3.
Reference examples 3
Reference examples 3 provide a kind of oligomeric peptides products of ox bone collagen albumen and stomach made of pharmaceutically acceptable auxiliary material
Floating tablet, the hydrophilic gel in the gastric floating tablet auxiliary material is that ratio of weight and number is 1:1 bean gum and the mixture of guar gum,
Other are consistent with embodiment 3.
Reference examples 4
Reference examples 4 provide a kind of oligomeric peptides products of ox bone collagen albumen and stomach made of pharmaceutically acceptable auxiliary material
Floating tablet, the hydrophilic gel in the gastric floating tablet auxiliary material is that ratio of weight and number is 1:The mixing of 1 guar gum and tamarind gum
Thing, other are consistent with embodiment 3.
Reference examples 5
Reference examples 5 provide a kind of oligomeric peptides products of ox bone collagen albumen and stomach made of pharmaceutically acceptable auxiliary material
Floating tablet, the hydrophilic gel in the gastric floating tablet auxiliary material is that ratio of weight and number is 1:The mixing of 1 bean gum and tamarind gum
Thing, other are consistent with embodiment 3.
Reference examples 6
Reference examples 6 provide a kind of oligomeric peptides products of ox bone collagen albumen and stomach made of pharmaceutically acceptable auxiliary material
Floating tablet, the foaming agent in the gastric floating tablet auxiliary material is that ratio of weight and number is 1:1:1:1 citric acid fatty glyceride, alkane
Base glycine betaine, polyethylene glycol monolaurate and diethanolamine of fat acyl mixture, other are consistent with embodiment 3.
Reference examples 7
Reference examples 7 provide a kind of oligomeric peptides products of ox bone collagen albumen and stomach made of pharmaceutically acceptable auxiliary material
Floating tablet, the foaming agent in the gastric floating tablet auxiliary material is that ratio of weight and number is 1:1:1 citric acid fatty glyceride, alkyl
The mixture of glycine betaine and polyethylene glycol monolaurate, other are consistent with embodiment 3.
Reference examples 8
Reference examples 8 provide a kind of oligomeric peptides products of ox bone collagen albumen and stomach made of pharmaceutically acceptable auxiliary material
Floating tablet, the foaming agent in the gastric floating tablet auxiliary material is that ratio of weight and number is 1:1:1:1 alkyl betaine, polyethyleneglycol
Laurate and diethanolamine of fat acyl mixture, other are consistent with embodiment 3.
Reference examples 9
Reference examples 9 provide a kind of oligomeric peptides products of ox bone collagen albumen and stomach made of pharmaceutically acceptable auxiliary material
Floating tablet, the removal foam stabilizer in the gastric floating tablet auxiliary material, other are consistent with embodiment 3.
Reference examples 10
Chinese medicine composition in embodiment 1 is adjusted to 4 for the ratio between parts by weight:1:6:1:5:1 keel, pig label grass,
Hoveniae semoveniae semen, chorion ovi, fenugreek and bitter orange flower, other and embodiment 1 are consistent, and ox bone collagen albumen oligopeptide system is made
Product.
Reference examples 11
The step for adding and preparing Chinese medicine composition will be removed the step for addition Chinese medicine composition in embodiment 1,
Other and embodiment 1 are consistent, and the oligomeric peptides products of ox bone collagen albumen without Chinese medical extract are made.
Reference examples 12
It will be added in embodiment 1 into the second enzymolysis liquid and ox bone ratio of weight and number be 1:The ratio of 150 Chinese medical extract
Example is adjusted to 1:200, other and embodiment 1 are consistent, and the oligomeric peptides products of ox bone collagen albumen are made.
Reference examples 13
It will be added in embodiment 1 into the second enzymolysis liquid and ox bone ratio of weight and number be 1:The ratio of 150 Chinese medical extract
Example is adjusted to 1:100, other and embodiment 1 are consistent, and the oligomeric peptides products of ox bone collagen albumen are made.
Experimental example 1
Dissolution rate comparative experiments
Using embodiment 1 as experimental group, with the stomach suspension piece that is provided in reference examples 1-9 as a control group, according to《China
People's republic's pharmacopeia》" Rotating shaker " of one of the Dissolution of Tablet determination method of 2010 editions records is to the molten of each group gastric floating tablet
Out-degree is measured, and specific assay method is as follows：
Using simulate the gastric juice as dissolution medium, 41 DEG C are heated to about, and is stirred continuously more than 5min under vacuum,
Obtain degassing dissolution medium；The simulate the gastric juice of degassed processing is measured respectively, is placed in 8 stripping rotors, every glass of 500mL, is treated molten
Go out medium temperature it is constant every group of sample takes 6 after 37 DEG C ± 0.5 DEG C, respectively put into 6 it is dry turns in basket, basket will be turned
Fall into stripping rotor, pay attention to there is not bubble on test sample surface, start dissolution rate instrument and be adjusted to 100r/min, record is every
The time of the complete dissolution of piece sample, and compare the dissolution rate of each group sample.Experimental result is shown in Table 1.
The complete dissolution time of 1. each group gastric floating tablet of table
As shown in Table 1, the dissolution time of embodiment 1 is significantly more than reference examples 1-9, can be seen that from reference examples 1- 5
Specific hydrophilic gel combination dissolution rate of the invention is minimum, either changes special ratios, specific components and reduction group
Part, the influence for dissolution time is very big.Slow-release tablet should slowly discharge active ingredient in dissolution medium, make in vivo
Certain level is held in the content certain time of active ingredient.It can be seen from the above that use hydrophilic gel provided by the invention
The gastric floating tablet of combination can be detained the long period in gastric juice, so as to improve utilization ratio of drug, can be seen that from reference examples 6-8
Use specific foaming agent combination provided by the invention to combine generation synergistic effect with collaboration hydrophilic gel to prolong, length is above-mentioned
Holdup time, holdup time of the gastric floating tablet in gastric juice is longer, from reference examples 9 as can be seen that foam stabilizer of the present invention not only has
The effect of foam stabilizing, can also cooperate with hydrophilic gel to combine, and further extend the above-mentioned holdup time.
Experimental example 2
External flotation property experiment
Using embodiment 1 as experimental group, with the stomach suspension piece that is provided in reference examples 1-9 as a control group, it is dense with 900mL
The hydrochloric acid solution spent for 0.1mol/L is dissolution medium, and temperature (37.0 ± 0.5) DEG C, rotating speed 100r/min, is clocked with stopwatch.
With gastric floating tablet be put into stripping rotor to tablet be floated to liquid level time be set to drift time T1, since drift start to experiment knot
Time during beam is set to continue flotation time T2.Experimental result is shown in Table 2.
The flotation time of 2. each group gastric floating tablet of table
As shown in Table 2, the flotation time of each group sample more than 12h, reach preferable effect；When playing drift of embodiment 1
Between be considerably shorter than reference examples 1-9.It can be seen from the above that the gastric floating tablet combined using hydrophilic gel provided by the invention can be
It is detained the long period in gastric juice, so as to improve utilization ratio of drug, and has used the stomach drift of foaming agent combination provided by the invention
Floating holdup time of the piece in gastric juice is longer, and can also further extend the above-mentioned holdup time using foam stabilizer.
Experimental example 3, the effect to osteoporosis
(1) symptom of osteoporosis
Bone density and bone mass decline, and micro- 0 structure of knot of bone is destroyed, and the increase of bone brittleness, easily fractures.
(2) crowd is tested
The subject of 250 symptoms for meeting osteoporosis, the age is between 25-60 Sui.
(3) it is grouped
250 osteoporosis subjects are randomly divided into 50 people of test group, 50 people of control group A, 50 people of control group B, right
According to 50 people of group C, 50 people of control group D；The oligomeric peptides products of ox bone collagen albumen that test group is prepared using the embodiment of the present invention 1；
Control group A takes the oligomeric peptides products of ox bone collagen albumen of the preparation of reference examples 10；Control group B takes the ox of the preparation of reference examples 11
The oligomeric peptides products of bone collagen；Control group C takes the oligomeric peptides products of ox bone collagen albumen of the preparation of reference examples 12；Control group D
Take the oligomeric peptides products of ox bone collagen albumen of the preparation of reference examples 13；Use 6 months.
(4) functional evaluation
It is effective：Clinical symptoms, body card integration reduce >=70%；The oligomeric peptides products of ox bone collagen albumen
Effectively：Clinical symptoms, body card integration reduce 50-70%；
It is invalid：Clinical symptoms, body card integration reduce≤50%
(5) result
The effect that table 3. adjusts osteoporosis compares
(6) conclusion
The oligomeric peptides products of ox bone collagen albumen prepared by test group, ox bone collagen albumen oligopeptide and Chinese medical extract lead to
Specific proportioning is crossed, every body card of patient's osteoporosis can be significantly improved.Control group A can be seen that when adjusting traditional Chinese medicine extraction
Chinese medicine proportioning in thing, effective percentage can decline therewith, and control group B is single ox bone collagen albumen oligopeptide, and effect reduces more
To be obvious, control group C and control group D change ox bone collagen albumen oligopeptide and Chinese medical extract proportioning, for efficient
It is also apparent to influence.Specific Chinese medicinal component in the embodiment of the present invention 1, specific ox bone collagen albumen oligopeptide is with
Medicament extract so that the effect of therebetween there is collaboration to absorb, discharge more active ingredients.
The preparation method of the oligomeric peptides products of ox bone collagen albumen provided by the invention, technique is advanced, process is reasonable, can grasp
The property made is strong, is easy to industrialized production, the oligomeric peptides products of ox bone collagen albumen prepared by this method have zhuanggu, increase bone density,
Promote bone growth healing, treatment of arthritis and other effects, the Chinese medicinal component of addition can be carried out with ox bone collagen albumen oligopeptide
Collaboration, it is easier to absorb, further enhancing zhuanggu strong bone, treat and prevent osteopathy the effect of, in addition, enzymolysis in enzyme group
Close and the selection of dosage so that enzymolysis efficiency and Degree of Enzymatic Hydrolysis are high, and in zymolyte amino chemicals type and quantity it is more,
It is in good taste.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
God and any modification, equivalent substitution, improvement and etc. within principle, done, should be included within the scope of protection of the invention.
Claims (8)
1. a kind of preparation method of the oligomeric peptides products of ox bone collagen albumen, it is characterised in that including at least following steps：
1) choose it is fresh clean after ox bone masking foil it is fully wrapped around after, be then placed in liquid nitrogen and cool down 1min, used after taking-up
Ox bone is crushed to 100 mesh by crusher, is added after extraction solution is extracted and is obtained solidliquid mixture；Wherein, the extraction is molten
Liquid is the mixed solution that solvent is water, and the concentration of each solute is respectively：Sodium hydroxide 100mM, sodium chloride 50mM, sucrose 10mM,
Extraction process carries out in the microwave environment of 400W；
2) mixed enzyme is added into solidliquid mixture, it is 6 to add newborn acid for adjusting pH value, is digested in 38 DEG C of isoperibol
60min, obtains the first enzymolysis liquid, and wherein mixed enzyme is that ratio of weight and number is 4:1:5:2 Collagenase, lipase, glycogen
Enzyme and nuclease mixing enzyme system；Keep temperature and pH value constant, the parts by weight such as addition and mixed enzyme into the first enzymolysis liquid
Protein incision enzyme, the second enzymolysis liquid is obtained after digesting 90min；
3) added into the second enzymolysis liquid and ox bone ratio of weight and number is 1:150 Chinese medical extract, is stirred continuously and is warming up to boiling
Rise, and keep 20min, be then down to room temperature, mixed extract is obtained after centrifugal filtration；Wherein, Chinese medical extract is by such as
Lower step is prepared：
A, dry Chinese medicine composition is taken to be crushed, wherein it is 5 that the Chinese medicine composition, which is the ratio between parts by weight,:2:10:4:3:1
Keel, pig label grass, hoveniae semoveniae semen, chorion ovi, fenugreek and bitter orange flower, then add volume fraction be 90% ethanol water
Refluxing extraction 5 times, adds ethanol water as 2 times of weight of dry Chinese medicine composition, return time 4h every time；Collect and merge
Extracting solution, is concentrated under reduced pressure into no alcohol, obtains thick medicinal extract；
B, the thick medicinal extract obtained by step a is suspended in the water of dry 2 times of volumes of Chinese medicine composition, then with petroleum ether extraction 3
Secondary, the amount for adding petroleum ether every time is 0.3 times of volume of thick medicinal extract, and organic phase is merged after extraction, dry, is concentrated under reduced pressure,
Obtain Chinese medicine crude extract；
C. the Chinese medicine crude extract obtained by step b is dissolved in methyl butyrate with the concentration of 1.0g/ml, upper silica gel column chromatography；
D. gradient elution：Elution flow is 6ml/min, first elutes 5 column volumes with petroleum ether, removes flow point；Volume ratio is used again
For 3:1 petroleum ether and dichloromethane mixed liquor elutes 6 column volumes, collects flow point；It is again 5 with volume ratio:3:1 oil
The mixed liquor of ether, dichloromethane and methyl butyrate elutes 8 column volumes, removes flow point；It is again 4 with volume ratio:3:2 oil
The mixed liquor of ether, dichloromethane and methyl butyrate elutes 20 column volumes, collects flow point, and the flow point of collection is merged, and decompression is dense
Contracting, up to Chinese medical extract；
4) after mixed extract is separated, concentrated and dried, the oligomeric peptides products of ox bone collagen albumen are obtained.
2. the oligomeric peptides products of ox bone collagen albumen as claimed in claim 1 are floated with stomach made of pharmaceutically acceptable auxiliary material
Piece, it is characterised in that further include auxiliary material, the ratio of weight and number of the oligomeric peptides products of the ox bone collagen albumen and auxiliary material is 1:6, institute
Stating auxiliary material includes the component of following parts by weight：20 parts of hydrophilic gel, 30 parts of filler, 2 parts of cosolvent, 4 parts of foaming agent, profit
2 parts of 2 parts of lubrication prescription and foam stabilizer.
3. gastric floating tablet as claimed in claim 2, it is characterised in that it is 1 that the hydrophilic gel, which includes ratio of weight and number,:3:
2.5 bean gum, guar gum and tamarind gum.
4. gastric floating tablet as claimed in claim 3, it is characterised in that it is 1 that the filler, which includes ratio of weight and number,:3:4
Crospovidone, vanillic aldehyde and xylitol.
5. gastric floating tablet as claimed in claim 4, it is characterised in that it is 1 that the cosolvent, which includes ratio of weight and number,:2:3 is poly-
Vinylpyrrolidone, heparin sodium and leucine.
6. gastric floating tablet as claimed in claim 5, it is characterised in that it is 1 that the foaming agent, which includes ratio of weight and number,:4:2:3
Citric acid fatty glyceride, alkyl betaine, polyethylene glycol monolaurate and diethanolamine of fat acyl.
7. gastric floating tablet as claimed in claim 6, it is characterised in that it is 2 that the lubricant, which includes ratio of weight and number,:3:5
Polyalphaolefin, magnesium laurylsulfate and behenic acid glyceryl ester.
8. gastric floating tablet as claimed in claim 7, it is characterised in that it is 1 that the foam stabilizer, which includes ratio of weight and number,:3:2
Polyethylene glycol, saponin and hydroxymethyl cellulose.
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| CN115251400A (en) * | 2022-06-16 | 2022-11-01 | 兆鑫堂(山东)生物科技有限公司 | Collagen peptide nutritional supplement composition capable of increasing bone density and application thereof |
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