CN108452296A - A kind of ox bone collagen protein peptides and preparation method thereof with antioxidant activity - Google Patents

A kind of ox bone collagen protein peptides and preparation method thereof with antioxidant activity Download PDF

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CN108452296A
CN108452296A CN201810645124.3A CN201810645124A CN108452296A CN 108452296 A CN108452296 A CN 108452296A CN 201810645124 A CN201810645124 A CN 201810645124A CN 108452296 A CN108452296 A CN 108452296A
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collagen protein
bone collagen
protein peptides
weight
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石丰
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/57—Birds; Materials from birds, e.g. eggs, feathers, egg white, egg yolk or endothelium corneum gigeriae galli
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/062—Ascomycota
    • A61K36/066—Clavicipitaceae
    • A61K36/068—Cordyceps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/11—Pteridophyta or Filicophyta (ferns)
    • A61K36/12—Filicopsida or Pteridopsida
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/35—Caprifoliaceae (Honeysuckle family)
    • A61K36/355—Lonicera (honeysuckle)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/41—Crassulaceae (Stonecrop family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/62—Nymphaeaceae (Water-lily family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
    • A61K36/704—Polygonum, e.g. knotweed
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/738—Rosa (rose)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/75—Rutaceae (Rue family)
    • A61K36/752—Citrus, e.g. lime, orange or lemon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/78—Saururaceae (Lizard's-tail family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/894—Dioscoreaceae (Yam family)
    • A61K36/8945—Dioscorea, e.g. yam, Chinese yam or water yam
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/90—Smilacaceae (Catbrier family), e.g. greenbrier or sarsaparilla
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/39—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps

Abstract

The ox bone collagen protein peptides and preparation method thereof with antioxidant activity that the present invention provides a kind of, technique is advanced, process is reasonable, operability is strong, it is easy to industrialized production, ox bone collagen protein peptides prepared by this method have invigorating qi for tranquilization, strengthening healthy qi, remove free radical, antioxidant activity, the effect of anti-aging, the Chinese medicinal component of addition can be cooperateed with ox bone collagen protein peptides, more easily absorb, effect is far superior to the effect superposition of single medicinal material, the rate that human body itself can be inhibited to generate free radicals, and the free radical generated in vivo can be cleared up to a certain extent, it is suitble to the middle-aged and the old to take for a long time, reach anti-aging, reduce the occurrence probability of disease caused by free radical, alleviate sub-health state, it improves the quality of living and working efficiency.

Description

A kind of ox bone collagen protein peptides and preparation method thereof with antioxidant activity
【Technical field】
The present invention relates to personal care product's technical field more particularly to a kind of ox bone collagen albumen with antioxidant activity Peptide and preparation method thereof.
【Background technology】
Human body is because of factors such as the continuous contacts with the external world, including breathing, outside contamination, radioactive ray irradiation constantly in people It is generated free radicals in body body.Scientific investigations showed that cancer, aging or Other diseases are mostly related with the generation of excessive free radicals Connection.
It is main in ox bone to contain collagen, have effects that high nutritive value, antioxidant activity, improve the immunity of the human body.Ox Bone usually enters human body in a manner of edible or decoction, and it is oligopeptide or amino that collagen, which will generally be digested liquid enzymolysis, Acid and be absorbed by organisms and utilize, therefore it is above-mentioned take the burden for not only resulting in people digest's system, and be not achieved and answer Some drug effects.
【Invention content】
In view of this, an embodiment of the present invention provides a kind of ox bone collagen protein peptides and its system with antioxidant activity Preparation Method.
This application provides a kind of ox bone collagen protein peptides with antioxidant activity, and the ox bone collagen protein peptides are under The raw material of row parts by weight is prepared：
2400-3600 parts of ox bone collagen protein peptide powder, 10-30 parts of rhodiola root, 5-15 parts of Polygonum multiflower knotweed, 3-7 parts of cordate houttuynia, Zhu Seven 10-20 parts of sand, 1-3 parts of bitter citrus immature flower, 10-20 parts of cordyceps sinensis, 4-12 parts of caulis lonicerae, 3-7 parts of Gorgon fruit, 4-12 parts of chinaroot greenbrier leaf, 8-16 parts of chorion ovi, 4-8 parts of the membrane of a chicken's gizzard, 3-7 parts of rhizome of cyrtomium, 5-15 parts of Chinese Incarvilla Herb, 2-6 parts of resina liquidamberis, 1-3 parts of the fruit of Cherokee rose.
Preferably, the ox bone collagen protein peptides are prepared by the raw material of following parts by weight：
3000 parts of ox bone collagen protein peptide powder, 20 parts of rhodiola root, 10 parts of Polygonum multiflower knotweed, 5 parts of cordate houttuynia, 15 parts of polygonum cill, turtle Turtle spend 2 parts, 15 parts of cordyceps sinensis, 8 parts of caulis lonicerae, 5 parts of Gorgon fruit, 8 parts of chinaroot greenbrier leaf, 12 parts of chorion ovi, 6 parts of the membrane of a chicken's gizzard, rhizome of cyrtomium 5 Part, 10 parts of Chinese Incarvilla Herb, 4 parts of resina liquidamberis, 2 parts of the fruit of Cherokee rose.
The present invention also provides a kind of preparation method of the ox bone collagen protein peptides with antioxidant activity, the method packets Include following steps：
1) rhodiola root, Polygonum multiflower knotweed, cordate houttuynia, polygonum cill, bitter citrus immature flower, cordyceps sinensis, caulis lonicerae, Gorgon euryale are chosen by weight Reality, chinaroot greenbrier leaf, chorion ovi, the membrane of a chicken's gizzard, rhizome of cyrtomium, Chinese Incarvilla Herb, resina liquidamberis and the fruit of Cherokee rose dry 30h, and dry powder is made in crushing；
2) dry powder made from step 1) is added in Chinese medicine extracting tank and presses 1 with deionized water:20 ratio is uniformly mixed, Stirring heats 6h, filters to obtain the first filtrate；
3) filter residue in the first filtrate is collected, by 1:Deionized water is added in 8 ratio, stirs, and 3h is heated under the conditions of 60 DEG C, The second filtrate is filtered to obtain, then the second filtrate is added in the first filtrate, is stirred, during distillation makes volume-diminished be obtained to 1/5 Medicine concentrate；
4) the concentrated liquid of tradition Chinese medicine made from step 3) is placed in fermentation tank, is added in fermentation tank and the bodies such as the concentrated liquid of tradition Chinese medicine Long-pending deionized water is sterilized after stirring evenly, and 0.2 part of alpha-amylase is added, stirs 1h under the conditions of 55 DEG C, is cooled to 50 DEG C, 0.2 part of pectase is added, stirs 2h, obtains zymotic fluid；
5) traditional Chinese medicine extraction is obtained after zymotic fluid made from step 4) being extracted, chromatograph, elutes, is concentrated under reduced pressure and dried Object；
6) ox bone collagen protein peptide powder will be taken by weight, and itself and step 5) will be mixed well to get ox bone collagen egg White peptide.
The present invention also provides a kind of oral preparation, the oral preparation is powder or capsule, the powder or capsule Agent includes above-mentioned ox bone collagen protein peptides and auxiliary material with antioxidant activity, the weight of the ox bone collagen protein peptides and auxiliary material Portion rate is：1:6.
Preferably, the oral preparation is powder, and the auxiliary material includes the ingredient of following parts by weight：Acrylic resin IV 5-15 parts, 10-30 parts of low-substituted hydroxypropyl cellulose, 1-5 parts of magnesium trisilicate, 1-5 parts of xylitol, behenic acid glyceryl ester 0.1- 0.5 part.
Preferably, the oral preparation is capsule, and the auxiliary material includes the ingredient of following parts by weight：Corn Ruan 4-10 Part, 1-3 parts of vanillic aldehyde, 2-5 parts of polacrilin potassium, 4-10 parts of lactose, 1-3 parts of heparin sodium and citric acid fatty glyceride 5-15 Part.
Preferably, the capsule is prepared by the following method：
A, ox bone collagen protein peptides are prepared；
B, corn Ruan of parts by weight is crushed to average grain diameter with micronizer and is less than 100 μm, volume fraction, which is added, is 90% ethanol water dissolves corn Ruan 5 times, and it is 2 times of weight of corn Ruan that ethanol water is added every time, obtains corn Ruan Yi Alcoholic solution；
C, ox bone collagen protein peptides made from step a are taken to be added in Wet mixed granulating machine, setting mixing speed 600r/ Min shreds speed 1200r/min, mixes 15min, then corn Ruan ethanol solution made from step b is added to wet-mixing Softwood is crossed 15 mesh and sieves wet granular processed by softwood processed in grain machine, and dry 90min, takes out under the conditions of wet granular is positioned over 50 DEG C, uses 15 mesh sieves, obtain hybrid particles；
D, vanillic aldehyde, polacrilin potassium, lactose, heparin sodium and the citric acid fatty glyceride and step c of parts by weight are taken Hybrid particles obtained are uniformly mixed；It is packed into capsule shells to get capsule according to the loading amount of 0.25 ± 10%/grain.
Further, ox bone collagen protein peptides described herein answering in preparing the product for antioxidant activity With.
A technical solution in above-mentioned technical proposal has the advantages that：
The ox bone collagen protein peptides and preparation method thereof with antioxidant activity that the present invention provides a kind of, technique is advanced, Process is reasonable, operability is strong, is easy to industrialized production, and ox bone collagen protein peptides prepared by this method have invigorating qi for tranquilization, help Positive solid removes the effect of free radical, antioxidant activity, anti-aging, and the Chinese medicinal component of addition can be with ox bone collagen albumen Gly-His-Lys are cooperateed with, it is easier to be absorbed, effect is far superior to the effect superposition of single medicinal material, and human body itself can be inhibited to be produced from By the rate of base, and the free radical generated in vivo can be cleared up to a certain extent, be suitble to mid-aged population or chronic patient long-term Take, reach anti-aging, reduce the occurrence probability of disease caused by free radical, alleviate sub-health state, improve the quality of living and Working efficiency.
【Specific implementation mode】
For a better understanding of the technical solution of the present invention, the embodiment of the present invention is described in detail below.
It will be appreciated that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Base Embodiment in the present invention, those of ordinary skill in the art obtained without creative efforts it is all its Its embodiment, shall fall within the protection scope of the present invention.
The term used in embodiments of the present invention is the purpose only merely for description specific embodiment, is not intended to be limiting The present invention.In the embodiment of the present invention and "an" of singulative used in the attached claims, " described " and "the" It is also intended to including most forms, unless context clearly shows that other meanings.
It should be appreciated that term "and/or" used herein is only a kind of incidence relation of description affiliated partner, indicate There may be three kinds of relationships, for example, A and/or B, can indicate：Individualism A, exists simultaneously A and B, individualism B these three Situation.In addition, character "/" herein, it is a kind of relationship of "or" to typically represent forward-backward correlation object.
Embodiment 1
Embodiment 1 provides a kind of ox bone collagen protein peptides with antioxidant activity, and the ox bone collagen protein peptides are under The raw material of row parts by weight is prepared：
Ox bone collagen protein peptide powder 2400g, rhodiola root 10g, Polygonum multiflower knotweed 5g, cordate houttuynia 3g, polygonum cill 10g, bitter citrus immature flower 1g, cordyceps sinensis 10g, caulis lonicerae 4g, Gorgon fruit 3g, chinaroot greenbrier leaf 4g, chorion ovi 8g, the membrane of a chicken's gizzard 4g, rhizome of cyrtomium 3g, Chinese Incarvilla Herb 5g, sweetgum Fat 2g, fruit of Cherokee rose 1g.
The preparation method of the ox bone collagen protein peptides is：
1) rhodiola root, Polygonum multiflower knotweed, cordate houttuynia, polygonum cill, bitter citrus immature flower, cordyceps sinensis, caulis lonicerae, Gorgon euryale are chosen by weight Reality, chinaroot greenbrier leaf, chorion ovi, the membrane of a chicken's gizzard, rhizome of cyrtomium, Chinese Incarvilla Herb, resina liquidamberis and the fruit of Cherokee rose dry 30h, and dry powder is made in crushing；
2) dry powder made from step 1) is added in Chinese medicine extracting tank and presses 1 with deionized water:20 ratio is uniformly mixed, Stirring heats 6h, filters to obtain the first filtrate；
3) filter residue in the first filtrate is collected, by 1:Deionized water is added in 8 ratio, stirs, and 3h is heated under the conditions of 60 DEG C, The second filtrate is filtered to obtain, then the second filtrate is added in the first filtrate, is stirred, during distillation makes volume-diminished be obtained to 1/5 Medicine concentrate；
4) the concentrated liquid of tradition Chinese medicine made from step 3) is placed in fermentation tank, is added in fermentation tank and the bodies such as the concentrated liquid of tradition Chinese medicine Long-pending deionized water is sterilized after stirring evenly, and 0.2 part of alpha-amylase is added, stirs 1h under the conditions of 55 DEG C, is cooled to 50 DEG C, 0.2 part of pectase is added, stirs 2h, obtains zymotic fluid；
5) traditional Chinese medicine extraction is obtained after zymotic fluid made from step 4) being extracted, chromatograph, elutes, is concentrated under reduced pressure and dried Object；
6) ox bone collagen protein peptide powder will be taken by weight, and itself and step 5) will be mixed well to get ox bone collagen egg White peptide.
Embodiment 2
Embodiment 2 provides a kind of ox bone collagen protein peptides with antioxidant activity, and the ox bone collagen protein peptides are under The raw material of row parts by weight is prepared：Ox bone collagen protein peptide powder 3000g, rhodiola root 20g, Polygonum multiflower knotweed 10g, cordate houttuynia 5g, Zhu Seven 15g of sand, bitter citrus immature flower 2g, cordyceps sinensis 15g, caulis lonicerae 8g, Gorgon fruit 5g, chinaroot greenbrier leaf 8g, chorion ovi 12g, the membrane of a chicken's gizzard 6g, rhizome of cyrtomium 5g, Chinese Incarvilla Herb 10g, resina liquidamberis 4g, fruit of Cherokee rose 2g.
The preparation method of ox bone collagen protein peptides and embodiment 1 are identical in embodiment 2.
Embodiment 3
Embodiment 3 provides a kind of ox bone collagen protein peptides with antioxidant activity, and the ox bone collagen protein peptides are under The raw material of row parts by weight is prepared：Ox bone collagen protein peptide powder 3600g, rhodiola root 30g, Polygonum multiflower knotweed 15g, cordate houttuynia 7g, Zhu Seven 20g of sand, it bitter citrus immature flower 3g, cordyceps sinensis 20g, caulis lonicerae 12g, Gorgon fruit 7g, chinaroot greenbrier leaf 12g, chorion ovi 16g, the membrane of a chicken's gizzard 8g, passes through Many 7g, Chinese Incarvilla Herb 15g, resina liquidamberis 6g, fruit of Cherokee rose 3g.
The preparation method of ox bone collagen protein peptides and embodiment 1 are identical in embodiment 3.
4 powder of embodiment
Embodiment 4 provides a kind of oral preparation, and oral preparation is powder, and the powder is by following parts by weight at grouping At：
Ox bone collagen protein peptides 2.85g made from embodiment 2, IV 5g of acrylic resin, low-substituted hydroxypropyl cellulose 10g, magnesium trisilicate 1g, xylitol 1g, behenic acid glyceryl ester 0.1g.
The preparation method of the powder is conventional method.
5 powder of embodiment
Embodiment 5 provides a kind of oral preparation, and oral preparation is powder, and the powder is by following parts by weight at grouping At：
Ox bone collagen protein peptides 6.05g made from embodiment 2, IV 10g of acrylic resin, low-substituted hydroxypropyl cellulose 20g, magnesium trisilicate 3g, xylitol 3g, behenic acid glyceryl ester 0.3g.
The preparation method of the powder is conventional method.
6 powder of embodiment
Embodiment 6 provides a kind of oral preparation, and oral preparation is powder, and the powder is by following parts by weight at grouping At：
Ox bone collagen protein peptides 9.25g made from embodiment 2, IV 15g of acrylic resin, low-substituted hydroxypropyl cellulose 30g, magnesium trisilicate 5g, xylitol 5g, behenic acid glyceryl ester 0.5g.
The preparation method of the powder is conventional method.
7 capsule of embodiment
Embodiment 7 provides a kind of oral preparation, and oral preparation is capsule, the capsule by following parts by weight ingredient Composition：
Ox bone collagen protein peptides 2.83g made from embodiment 2, corn Ruan 4g, vanillic aldehyde 1g, polacrilin potassium 2g, lactose 4g, heparin sodium 1g and citric acid fatty glyceride 5g.
The preparation method of the capsule is specific as follows：
A, ox bone collagen protein peptides are prepared；
B, corn Ruan of parts by weight is crushed to average grain diameter with micronizer and is less than 100 μm, volume fraction, which is added, is 90% ethanol water dissolves corn Ruan 5 times, and it is 2 times of weight of corn Ruan that ethanol water is added every time, obtains corn Ruan Yi Alcoholic solution；
C, ox bone collagen protein peptides made from step a are taken to be added in Wet mixed granulating machine, setting mixing speed 600r/ Min shreds speed 1200r/min, mixes 15min, then corn Ruan ethanol solution made from step b is added to wet-mixing Softwood is crossed 15 mesh and sieves wet granular processed by softwood processed in grain machine, and dry 90min, takes out under the conditions of wet granular is positioned over 50 DEG C, uses 15 mesh sieves, obtain hybrid particles；
D, vanillic aldehyde, polacrilin potassium, lactose, heparin sodium and the citric acid fatty glyceride and step c of parts by weight are taken Hybrid particles obtained are uniformly mixed；It is packed into capsule shells to get capsule according to the loading amount of 0.25 ± 10%/grain.
8 capsule of embodiment
Embodiment 8 provides a kind of oral preparation, and oral preparation is capsule, the capsule by following parts by weight ingredient Composition：
Ox bone collagen protein peptides 5.25g made from embodiment 2, corn Ruan 7g, vanillic aldehyde 2g, polacrilin potassium 3.5g, breast Sugared 7g, heparin sodium 2g and citric acid fatty glyceride 10g.
The preparation method and embodiment 7 of the capsule are identical.
9 capsule of embodiment
Embodiment 9 provides a kind of oral preparation, and oral preparation is capsule, the capsule by following parts by weight ingredient Composition：
Ox bone collagen protein peptides 8.17g made from embodiment 2, corn Ruan 10g, vanillic aldehyde 3g, polacrilin potassium 5g, lactose 10g, heparin sodium 3g and citric acid fatty glyceride 15g.
The preparation method and embodiment 7 of the capsule are identical.
It should be noted that the ox bone collagen protein peptides of the application are prepared via a method which：
1) choose the ox bone masking foil after fresh clean it is fully wrapped around after, be then placed in liquid nitrogen cooling 1min, take out Ox bone is crushed to 100 mesh with crusher afterwards, is added after extraction solution extracts and obtains solidliquid mixture；Wherein, described to carry It is the mixed solution that solvent is water to take solution, and the concentration of each solute is respectively：Sodium hydroxide 100mM, sodium chloride 50mM, sucrose 10mM, extraction process carry out in the microwave environment of 400W；
2) mixed enzyme is added into solidliquid mixture, it is 6 that newborn acid for adjusting pH value, which is added, is digested in 38 DEG C of isoperibol 60min, obtains the first enzymolysis liquid, and it is 4 that wherein mixed enzyme, which is weight part ratio,:1:5:2 Collagenase, lipase, glycogen Enzyme and nuclease mix enzyme system；It keeps temperature and pH value constant, is added into the first enzymolysis liquid and the parts by weight such as mixed enzyme Protein incision enzyme obtains the second enzymolysis liquid after digesting 90min；
3) after the second enzymolysis liquid being detached, concentrated and dried, the oligomeric peptides products of ox bone collagen albumen are obtained.
Reference examples 1
Reference examples 1 provide a kind of ox bone collagen protein peptides with antioxidant activity, and the ox bone collagen protein peptides are under The raw material of row parts by weight is prepared：Ox bone collagen protein peptide powder 3000g, rhodiola root 20g, Polygonum multiflower knotweed 10g, cordate houttuynia 5g, Zhu Seven 15g of sand, kadsura root 2g, cordyceps sinensis 15g, caulis lonicerae 8g, Gorgon fruit 5g, chinaroot greenbrier leaf 8g, chorion ovi 12g, the membrane of a chicken's gizzard 6g, Rhizome of cyrtomium 5g, Chinese Incarvilla Herb 10g, resina liquidamberis 4g, fruit of Cherokee rose 2g.
The preparation method of ox bone collagen protein peptides and embodiment 1 are identical in comparative example 1.
Reference examples 2
Reference examples 2 provide a kind of ox bone collagen protein peptides with antioxidant activity, and the ox bone collagen protein peptides are under The raw material of row parts by weight is prepared：Ox bone collagen protein peptide powder 3000g, rhodiola root 20g, Polygonum multiflower knotweed 10g, cordate houttuynia 5g, Zhu Seven 15g of sand, bitter citrus immature flower 2g, cordyceps sinensis 15g, caulis lonicerae 8g, Gorgon fruit 5g, chinaroot greenbrier leaf 8g, riparian greenbrier root and rhizome 12g, the membrane of a chicken's gizzard 6g, rhizome of cyrtomium 5g, Chinese Incarvilla Herb 10g, resina liquidamberis 4g, fruit of Cherokee rose 2g.
The preparation method of ox bone collagen protein peptides and embodiment 1 are identical in comparative example 2.
Reference examples 3
Reference examples 3 provide a kind of ox bone collagen protein peptides with antioxidant activity, and the ox bone collagen protein peptides are under The raw material of row parts by weight is prepared：Ox bone collagen protein peptide powder 3000g, rhodiola root 20g, Polygonum multiflower knotweed 10g, cordate houttuynia 5g, Zhu Seven 15g of sand, bitter citrus immature flower 2g, cordyceps sinensis 15g, caulis lonicerae 8g, Gorgon fruit 5g, chinaroot greenbrier leaf 8g, chorion ovi 12g, the membrane of a chicken's gizzard 6g, rhizome of cyrtomium 5g, Chinese Incarvilla Herb 10g, cudrania root 4g, fruit of Cherokee rose 2g.
The preparation method of ox bone collagen protein peptides and embodiment 1 are identical in comparative example 3.
Reference examples 4
Reference examples 4 provide a kind of ox bone collagen protein peptides with antioxidant activity, and the ox bone collagen protein peptides are under The raw material of row parts by weight is prepared：Ox bone collagen protein peptide powder 3000g, polyporus lucidus 20g, oldenlandia diffusa 20g, the distant five tastes 20g, ginseng 50g, paris polyphylla 2g, dalbergia wood 6g, saururus chinensis 10g, summer dry ball 20g, hoveniae semoveniae semen 10g, emblic 20g, field pennycress 4g, courage Southern Star 6g, rhizoma imperatae 20g, cordyceps sinensis 5g, Yuan root 6g, Caulis Spatholobi 2g, Gorgon fruit 4g, Sculellaria barbata 6g, christina loosestrife herb 20g.
The preparation method of ox bone collagen protein peptides selects conventional method to prepare in comparative example 4, i.e., by all important component powder It is broken then to be mixed with ox bone collagen protein peptide powder to obtain the ox bone collagen protein peptides in comparative example 4 for preset mesh number.
5 powder of reference examples
Reference examples 5 provide a kind of oral preparation, and oral preparation is powder, and the powder is by following parts by weight at grouping At：
Ox bone collagen protein peptides 6.05g made from embodiment 2, IV 10g of acrylic resin, low-substituted hydroxypropyl cellulose 20g, magnesium trisilicate 3g, xylitol 3g, behenic acid glyceryl ester 0.3g.
The preparation method of the powder is conventional method.
6 powder of reference examples
Reference examples 6 provide a kind of oral preparation, and oral preparation is powder, and the powder is by following parts by weight at grouping At：
Ox bone collagen protein peptides 6.05g, lactose 10g, low-substituted hydroxypropyl cellulose 20g, three silicon made from embodiment 2 Sour calcium 3g, xylitol 3g, polyalphaolefin 0.3g.
The preparation method of the powder is conventional method.
7 capsule of reference examples
Reference examples 7 provide a kind of oral preparation, and oral preparation is capsule, the capsule by following parts by weight ingredient Composition：
Ox bone collagen protein peptides 5.25g made from embodiment 2, corn Ruan 7g, vanillic aldehyde 2g, polacrilin potassium 3.5g, breast Sugared 7g, polyvinylpyrrolidone 2g and citric acid fatty glyceride 10g.
The preparation method and embodiment 7 of the capsule are identical.
8 capsule of reference examples
Reference examples 8 provide a kind of oral preparation, and oral preparation is capsule, the capsule by following parts by weight ingredient Composition：
Ox bone collagen protein peptides 5.25g made from embodiment 2, corn Ruan 7g, vanillic aldehyde 2g, polacrilin potassium 3.5g, breast Sugared 7g, heparin sodium 2g and polyethylene glycol monolaurate 10g.
The preparation method and embodiment 7 of the capsule are identical.
Antioxidant activity is tested
Healthy mice is chosen, mouse is divided into experiment 1-3 groups, compares 1-3 groups, model group, blank group, every group 10.Examination The ox bone collagen protein peptides gavage that 1-3 groups give embodiment 1-3 is tested, control 1-3 groups give the ox bone collagen albumen of reference examples 1-3 Peptide gavage, 1 group of model and blank group give the physiological saline of same dose, and dosage is 50mg/ days/, successive administration 15 days, mouse overnight fasting before last dose, last dose equal gavage 0.05mg/ of each group in addition to blank control group after 0.5 hour Bromobenzene solution, puts to death animal, every mouse takes one piece of hepatic tissue of same leaf liver same area, weighs one after 24 hours Gram, liver homogenate is quickly made at 0 DEG C, measures the content of MDA and GSH-Px in each group mouse liver, the results are shown in Table 1.
Content of the 1 each group ox bone collagen protein peptides of table to MDA and GSH-Px
Group MDA/nmol*mgprotein-1 GSH-Px/U*mgprotein-1
Blank group 3.75±0.31 994±108
Model group 4.91±0.46** 721±99**
Test 1 group 3.12±0.29☆☆ 921±101☆☆
Test 2 groups 2.87±0.27☆☆△△ 985±131☆☆△△
Test 3 groups 3.31±0.30☆☆△ 908±116☆☆△
Compare 1 group 3.62±0.35☆ 873±73☆
Compare 2 groups 3.70±0.41☆☆ 856±69☆☆
Compare 3 groups 3.51±0.39☆☆ 811±62☆☆
From table 1 it follows that compared with blank group, the MDA contents in model group mouse liver significantly increase, GSH- Px vigor significantly reduces, and illustrates modeling success.It is compared with control 1-3 groups, experiment 1-3 groups are to improving GSH-Px activity and reducing MDA contents are more notable, again the most notable with 2 groups of experiment among these, therefore can be seen that the ox bone collagen protein peptides of the present invention can Effectively improving bromobenzene causes the GSH-Px in hepatotoxicity Mice Body active, reduces the content of end product of lipid hyperoxygen MDA, has Preferable antioxidation.
Hydroscopicity is tested
The powder of Example 4-6 and reference examples 4-6 respectively, is positioned over natural cooling in drier, by the sample after drying Product are positioned in the weighing cup with cover after drying, record the weight of weighing cup and sample, the weighing cup for filling drying sample is put Be placed in moisture maintainer, keep 60% constant humidity condition, respectively 2,4,6,8, sample is weighed afterwards for 24 hours, and calculate moisture absorption Rate.As a result such as table 2.
Hydroscopicity (%)=100 (Wa-Wb)/Wb × 100%；
Wherein, Wa is the quality before sample is placed, and Wb is the quality after sample is placed.
The hydroscopicity test result of 2 inventive samples of table
As shown in Table 2, the hydroscopicity of embodiment 4-6 is significantly lower than reference examples 5-6, it was demonstrated that the present invention passes through in powder The mixture of acrylic resin IV, low-substituted hydroxypropyl cellulose, magnesium trisilicate, xylitol, behenic acid glyceryl ester is added, it can It significantly to reduce the hygroscopicity of powder provided by the invention, prevents its moisture absorption hardened, when deletion or replaces any of which Ingredient can improve the hygroscopicity of powder provided by the invention.
Content range pattern
The capsule of Example 7-9 and each 10 parts of the capsule of reference examples 7-8, according to version in 2015《Chinese Pharmacopoeia》In Method measure the uniformity of dosage units of its content, the results are shown in Table 3.
The uniformity of dosage units testing result of 3 capsule of the present invention of table
As can be seen from Table 3, the content for the capsule that 7-9 of the embodiment of the present invention is provided has good content uniform Degree, it was demonstrated that when addition corn Ruan, vanillic aldehyde, polacrilin potassium, lactose, heparin sodium and citric acid fatty acid glycerine in capsule When the mixture of ester, can significantly improve the uniformity of dosage units of capsule, when to auxiliary material provided by the invention carry out delete or When replacement, the uniformity of dosage units of capsule obtained is not inconsistent standardization, the uniformity of dosage units of content in the capsule of embodiment 8 It is apparently higher than embodiment 7 and embodiment 9, it was demonstrated that the preparation method of capsule provided by the invention can effectively improve capsule The uniformity of dosage units of agent content.
The ox bone collagen protein peptides and preparation method thereof with antioxidant activity that the present invention provides a kind of, technique is advanced, Process is reasonable, operability is strong, is easy to industrialized production, and ox bone collagen protein peptides prepared by this method have invigorating qi for tranquilization, help Positive solid removes the effect of free radical, antioxidant activity, anti-aging, and the Chinese medicinal component of addition can be with ox bone collagen albumen Gly-His-Lys are cooperateed with, it is easier to be absorbed, effect is far superior to the effect superposition of single medicinal material, and human body itself can be inhibited to be produced from By the rate of base, and the free radical generated in vivo can be cleared up to a certain extent, person in middle and old age group or chronic patient is suitble to take for a long time With reaching anti-aging, reduce the occurrence probability of disease caused by free radical, alleviate sub-health state, improve the quality of living and work Make efficiency.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention With within principle, any modification, equivalent substitution, improvement and etc. done should be included within the scope of protection of the invention god.

Claims (8)

1. a kind of ox bone collagen protein peptides with antioxidant activity, which is characterized in that the ox bone collagen protein peptides are by following The raw material of parts by weight is prepared：
2400-3600 parts of ox bone collagen protein peptide powder, 10-30 parts of rhodiola root, 5-15 parts of Polygonum multiflower knotweed, 3-7 parts of cordate houttuynia, polygonum cill 10-20 parts, 1-3 parts of bitter citrus immature flower, 10-20 parts of cordyceps sinensis, 4-12 parts of caulis lonicerae, 3-7 parts of Gorgon fruit, 4-12 parts of chinaroot greenbrier leaf, phoenix 8-16 parts of clothing, 4-8 parts of the membrane of a chicken's gizzard, 3-7 parts of rhizome of cyrtomium, 5-15 parts of Chinese Incarvilla Herb, 2-6 parts of resina liquidamberis, 1-3 parts of the fruit of Cherokee rose.
2. the ox bone collagen protein peptides according to claim 1 with antioxidant activity, which is characterized in that the bone glue Former protein peptides are prepared by the raw material of following parts by weight：
3000 parts of ox bone collagen protein peptide powder, 20 parts of rhodiola root, 10 parts of Polygonum multiflower knotweed, 5 parts of cordate houttuynia, 15 parts of polygonum cill, bitter citrus immature flower 2 Part, 15 parts of cordyceps sinensis, 8 parts of caulis lonicerae, 5 parts of Gorgon fruit, 8 parts of chinaroot greenbrier leaf, 12 parts of chorion ovi, 6 parts of the membrane of a chicken's gizzard, 5 parts of rhizome of cyrtomium, Chinese Incarvilla Herb 10 parts, 4 parts of resina liquidamberis, 2 parts of the fruit of Cherokee rose.
3. a kind of preparation method of ox bone collagen protein peptides with antioxidant activity as described in right 1 or 2, feature exist In described method includes following steps：
1) rhodiola root, Polygonum multiflower knotweed, cordate houttuynia, polygonum cill, bitter citrus immature flower, cordyceps sinensis, caulis lonicerae, Gorgon fruit, greenbrier are chosen by weight Chinaroot greenbrier leaf, chorion ovi, the membrane of a chicken's gizzard, rhizome of cyrtomium, Chinese Incarvilla Herb, resina liquidamberis and the fruit of Cherokee rose dry 30h, and dry powder is made in crushing；
2) dry powder made from step 1) is added in Chinese medicine extracting tank and presses 1 with deionized water:20 ratio is uniformly mixed, and is stirred It mixes, heats 6h, filter to obtain the first filtrate；
3) filter residue in the first filtrate is collected, by 1:Deionized water is added in 8 ratio, stirs, and 3h is heated under the conditions of 60 DEG C, filters The second filtrate, then by the second filtrate be added the first filtrate in, be stirred, distillation make volume-diminished to 1/5 Chinese medicine it is dense Contracting liquid；
4) the concentrated liquid of tradition Chinese medicine made from step 3) is placed in fermentation tank, is added in fermentation tank and the concentrated liquid of tradition Chinese medicine is isometric Deionized water is sterilized after stirring evenly, and 0.2 part of alpha-amylase is added, stirs 1h under the conditions of 55 DEG C, is cooled to 50 DEG C, 0.2 part of pectase is added, stirs 2h, obtains zymotic fluid；
5) Chinese medical extract is obtained after zymotic fluid made from step 4) being extracted, chromatograph, elutes, is concentrated under reduced pressure and dried；
6) ox bone collagen protein peptide powder will be taken by weight, and itself and step 5) will be mixed well to get ox bone collagen protein peptides.
4. a kind of oral preparation, the oral preparation is powder or capsule, the powder or capsule include claim 1 or The ox bone collagen protein peptides and auxiliary material with antioxidant activity described in 2, the parts by weight of the ox bone collagen protein peptides and auxiliary material Counting ratio is：1:6.
5. oral preparation according to claim 4, which is characterized in that the oral preparation is powder, and the auxiliary material includes The ingredient of following parts by weight：IV 5-15 parts of acrylic resin, 10-30 parts of low-substituted hydroxypropyl cellulose, magnesium trisilicate 1-5 Part, 1-5 parts of xylitol, 0.1-0.5 parts of behenic acid glyceryl ester.
6. the oral preparation described in claim 4, which is characterized in that the oral preparation is capsule, and the auxiliary material includes such as The ingredient of lower parts by weight：4-10 parts of corn Ruan, 1-3 parts of vanillic aldehyde, 2-5 parts of polacrilin potassium, 4-10 parts of lactose, heparin sodium 1- 3 parts and 5-15 parts of citric acid fatty glyceride.
7. oral preparation as claimed in claim 6, which is characterized in that the capsule is prepared by the following method：
A, ox bone collagen protein peptides are prepared；
B, corn Ruan of parts by weight is crushed to average grain diameter with micronizer and is less than 100 μm, it is 90% that volume fraction, which is added, Ethanol water by corn Ruan dissolve 5 times, every time be added ethanol water be 2 times of weight of corn Ruan, it is molten to obtain corn Ruan's ethyl alcohol Liquid；
C, it takes ox bone collagen protein peptides made from step a to be added in Wet mixed granulating machine, sets mixing speed 600r/min, Speed 1200r/min is shredded, mixes 15min, then corn Ruan ethanol solution made from step b is added to Wet mixed granulating machine In softwood processed, softwood is crossed into 15 mesh and sieves wet granular processed, dry 90min, takes out, with 15 mesh under the conditions of wet granular is positioned over 50 DEG C Whole grain is sieved, hybrid particles are obtained；
D, vanillic aldehyde, polacrilin potassium, lactose, heparin sodium and the citric acid fatty glyceride and step c for taking parts by weight are made Hybrid particles be uniformly mixed；It is packed into capsule shells to get capsule according to the loading amount of 0.25 ± 10%/grain.
8. application of the ox bone collagen protein peptides as claimed in claim 1 or 2 in preparing the product for antioxidant activity.
CN201810645124.3A 2018-06-21 2018-06-21 A kind of ox bone collagen protein peptides and preparation method thereof with antioxidant activity Withdrawn CN108452296A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109744543A (en) * 2019-01-09 2019-05-14 长沙市惠瑞生物科技有限公司 A kind of bone collagen peptide composite powder and preparation method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109744543A (en) * 2019-01-09 2019-05-14 长沙市惠瑞生物科技有限公司 A kind of bone collagen peptide composite powder and preparation method thereof

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Application publication date: 20180828