CN108770989A - A kind of preparation method of the ox bone collagen protein peptides with pre- preventing bone rarefaction effect - Google Patents
A kind of preparation method of the ox bone collagen protein peptides with pre- preventing bone rarefaction effect Download PDFInfo
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- CN108770989A CN108770989A CN201810645123.9A CN201810645123A CN108770989A CN 108770989 A CN108770989 A CN 108770989A CN 201810645123 A CN201810645123 A CN 201810645123A CN 108770989 A CN108770989 A CN 108770989A
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- parts
- bone
- collagen protein
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- protein peptides
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Classifications
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- A61K36/18—Magnoliophyta (angiosperms)
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Abstract
The present invention provides a kind of preparation methods of the ox bone collagen protein peptides with pre- preventing bone rarefaction effect, technique is advanced, process is reasonable, operability is strong, it is easy to industrialized production, ox bone collagen protein peptides prepared by this method have zhuanggu, it fills blood, tonifying spleen and stomach, kidney tonifying, the effect of strengthening healthy qi, the Chinese medicinal component of addition can be cooperateed with ox bone collagen protein peptides, more easily absorb, effect is far superior to the effect superposition of single medicinal material, human bone mineral density can be increased, it is suitble to patient with bone disease, skeletal injury person or bone density loss crowd take for a long time, reduce calcium loss, enhance bone density, also there is conditioning chronic disease, the effect of improving the quality of living.
Description
【Technical field】
The present invention relates to personal care product's technical field more particularly to a kind of bone glues with pre- preventing bone rarefaction effect
The preparation method of former protein peptides.
【Background technology】
Osteoporosis is a kind of systemic skeletal disease caused by many reasons, it is reduced with bone amount, under bone density
Drop, the degeneration of bone tissue microstructure are characterized, a kind of degeneration for causing bone brittleness to increase and occur with the increase at age
Osteopathy.Osteoporosis is a kind of geriatric disease, and women morbidity is higher than male, is to cause dysostosis and shape by many reasons
At.Bone density is an important symbol of bone mass, reflects the degree of osteoporosis, predict osteoporotic fracture it is important according to
According to.Osteoporosis is as modern civilization diseases, it has also become one of global important public hygiene problem.According to incomplete system
The senile osteoporosis disease incidence of meter, China 60 years old or more is about 59.89%, increases bone density, effectively treatment sclerotin is dredged
Loose disease has become the important topic of people's extensive concern.
It is main in ox bone to contain collagen, have effects that high nutritive value, zhuanggu, improve the immunity of the human body.Ox bone is usual
Enter human body in a manner of edible or decoction, collagen to be generally digested liquid enzymolysis as oligopeptide or amino acid and by
Body is absorbed and is utilized, and older population is common weakness of the spleen and the stomach or has gastrointestinal reaction, therefore the activity of digestive enzyme is low, digestion
Can force difference, therefore it is above-mentioned take the burden for not only resulting in people digest's system, and due drug effect is not achieved.
And ox bone collagen albumen is obtained into small-molecular peptides by processing and be aided with Chinese medicine composition can effectively overcome it is above-mentioned
Problem carrys out Gospel for mankind's health care belt.Therefore, the high ox bone collagen albumen of a kind of suitable industrialized production of invention, product quality
The preparation method of peptide is those skilled in the art's technical problem urgently to be resolved hurrily.
【Invention content】
In view of this, an embodiment of the present invention provides a kind of ox bone collagen protein peptides with pre- preventing bone rarefaction effect
Preparation method.
This application provides a kind of preparation methods of the ox bone collagen protein peptides with pre- preventing bone rarefaction effect, at least wrap
Include following steps：
1) ox bone after fresh clean is chosen, places it in cooking liquor and is steamed under 150 DEG C of boiling temperature at temperature
3h is boiled, cooling 15min at a temperature of then being pulled out the ox bone after boiling from cooking liquor and being placed in -80 DEG C while hot, after taking-up
Ox bone is crushed into the bovine bone powder for 50 mesh with crusher, bovine bone powder is rejoined in cooking liquor and obtains bovine bone powder suspension；Its
In, the cooking liquor is the mixed solution that solvent is water, and the concentration of each solute is respectively：Sodium hydroxide 80mM, sodium chloride 50mM,
10 mM of sulfamic acid sodium, lactose 10mM；
2) mixed enzyme is added into bovine bone powder suspension, it is 5.8 that lemon acid for adjusting pH value, which is added, in 37 DEG C of isoperibol
Middle enzymolysis 90min, obtains the first enzymolysis liquid, and it is 8 that wherein mixed enzyme, which is weight part ratio,:3:5:1 clostridiopetidase A, lipase, sugar
First enzyme and nuclease mix enzyme system；It keeps temperature and pH value constant, is added into the first enzymolysis liquid and the parts by weight such as mixed enzyme
Proteolytic enzyme, digest 120min after obtain the second enzymolysis liquid；
3) after the second enzymolysis liquid being detached, concentrated and dried, ox bone collagen protein peptide powder is obtained；
4) by ox bone collagen protein peptide powder and Chinese medical extract with weight part ratio 25:1 ratio, which is uniformly mixed, to be obtained
Ox bone collagen protein peptides；Wherein, the preparation method of the Chinese medical extract includes the following steps：
A, 20 parts of the rhizome of davallia, 5 parts of Herba Epimedii, 10 parts of ginseng, 5 parts of root of Aucklandia lappa Decne, 10 parts of the fruit of Chinese wolfberry, 15 parts of cordyceps sinensis, flea are taken
Not 5 parts, 5 parts of Gorgon fruit, 8 parts of dark plum, 12 parts of the fruit of Cherokee rose, 6 parts of arisaema cum bile, 5 parts, nine Ns 10 parts of power of rhizome of cyrtomium, umbellate pore furgus fragrant 4 parts and summer
Dry powder is made in 2 parts of drying 30h of withered ball, crushing；
B, dry powder made from step a is added in Chinese medicine extracting tank and presses 1 with deionized water:20 ratio is uniformly mixed,
Stirring heats 6h, filters to obtain the first filtrate；
C, the filter residue in the first filtrate is collected, by 1:Deionized water is added in 8 ratio, stirs, and 3h is heated under the conditions of 60 DEG C,
The second filtrate is filtered to obtain, then the second filtrate is added in the first filtrate, is stirred, during distillation makes volume-diminished be obtained to 1/5
Medicine concentrate；
D, the concentrated liquid of tradition Chinese medicine made from step c is placed in fermentation tank, is added in fermentation tank and the bodies such as the concentrated liquid of tradition Chinese medicine
Long-pending deionized water is sterilized after stirring evenly, and 0.2 part of alpha-amylase is added, stirs 1h under the conditions of 55 DEG C, is cooled to 50
DEG C, 0.2 part of pectase is added, stirs 2h, obtains zymotic fluid；
E, Chinese medical extract is obtained after being extracted, chromatographed, eluted, be concentrated under reduced pressure and being dried to zymotic fluid.
Further, present invention also provides a kind of ox bone collagen protein peptides and pharmacy with pre- preventing bone rarefaction effect
Electuary made of upper acceptable auxiliary material, further includes auxiliary material, and the weight part ratio of the ox bone collagen protein peptides and auxiliary material is 1:
4, the auxiliary material includes the ingredient of following parts by weight：200 parts of beta-cyclodextrin, 20 parts of steviol glycoside, 5 parts of citric acid, sesame flower
10 parts of powder, 8 parts of seabed coconut.
Further, present invention also provides a kind of ox bone collagen protein peptides and pharmacy with pre- preventing bone rarefaction effect
Capsule made of upper acceptable auxiliary material, further includes auxiliary material, the weight part ratio of the ox bone collagen protein peptides and auxiliary material is
1:6, the auxiliary material includes the ingredient of following parts by weight：4-10 parts of corn Ruan, 1-3 parts of vanillic aldehyde, 2-5 parts of polacrilin potassium,
5-15 parts of 4-10 parts of lactose, 1-3 parts of heparin sodium and citric acid fatty glyceride.
Further, the capsule is prepared by the following method：
A, ox bone collagen protein peptides are prepared；
B, corn Ruan of parts by weight is crushed to average grain diameter with micronizer and is less than 100 μm, volume fraction, which is added, is
90% ethanol water dissolves corn Ruan 5 times, and it is 2 times of weight of corn Ruan that ethanol water is added every time, obtains corn Ruan Yi
Alcoholic solution；
C, ox bone collagen protein peptides made from step a are taken to be added in Wet mixed granulating machine, setting mixing speed 600r/
Min shreds speed 1200r/min, mixes 15min, then corn Ruan ethanol solution made from step b is added to wet-mixing
Softwood is crossed 15 mesh and sieves wet granular processed by softwood processed in grain machine, and dry 90min, takes out under the conditions of wet granular is positioned over 50 DEG C, uses
15 mesh sieves, obtain hybrid particles；
D, vanillic aldehyde, polacrilin potassium, lactose, heparin sodium and the citric acid fatty glyceride and step c of parts by weight are taken
Hybrid particles obtained are uniformly mixed；It is packed into capsule shells to get capsule according to the loading amount of 0.25 ± 10%/grain.
Further, ox bone collagen protein peptides described herein answering in preparing the product for pre- preventing bone rarefaction
With.
A technical solution in above-mentioned technical proposal has the advantages that：
The present invention provides a kind of ox bone collagen protein peptides preparation method with pre- preventing bone rarefaction effect, technique is first
Into, process is reasonable, operability is strong, is easy to industrialized production, ox bone collagen protein peptides prepared by this method have zhuanggu, tonifying Qi
The Chinese medicinal component of the effect of blood, tonifying spleen and stomach, kidney tonifying, strengthening healthy qi, addition can be cooperateed with ox bone collagen protein peptides, more be held
It easily absorbs, effect is far superior to the effect superposition of single medicinal material, can increase human bone mineral density, is suitble to patient with bone disease, bone damage
The wounded or bone density loss crowd take for a long time, reduce calcium loss, enhance bone density, also have conditioning chronic disease, improve life
The effect of quality.
【Specific implementation mode】
For a better understanding of the technical solution of the present invention, the embodiment of the present invention is described in detail below.
It will be appreciated that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art obtained without creative efforts it is all its
Its embodiment, shall fall within the protection scope of the present invention.
The term used in embodiments of the present invention is the purpose only merely for description specific embodiment, is not intended to be limiting
The present invention.In the embodiment of the present invention and "an" of singulative used in the attached claims, " described " and "the"
It is also intended to including most forms, unless context clearly shows that other meanings.
It should be appreciated that term "and/or" used herein is only a kind of incidence relation of description affiliated partner, indicate
There may be three kinds of relationships, for example, A and/or B, can indicate：Individualism A, exists simultaneously A and B, individualism B these three
Situation.In addition, character "/" herein, it is a kind of relationship of "or" to typically represent forward-backward correlation object.
Embodiment 1
Embodiment 1 provides a kind of preparation method of the ox bone collagen protein peptides with pre- preventing bone rarefaction effect, special
Sign is, includes at least following steps：
1) ox bone after fresh clean is chosen, places it in cooking liquor and is steamed under 150 DEG C of boiling temperature at temperature
3h is boiled, cooling 15min at a temperature of then being pulled out the ox bone after boiling from cooking liquor and being placed in -80 DEG C while hot, after taking-up
Ox bone is crushed into the bovine bone powder for 50 mesh with crusher, bovine bone powder is rejoined in cooking liquor and obtains bovine bone powder suspension；Its
In, the cooking liquor is the mixed solution that solvent is water, and the concentration of each solute is respectively：Sodium hydroxide 80mM, sodium chloride 50mM,
10 mM of sulfamic acid sodium, lactose 10mM；
2) mixed enzyme is added into bovine bone powder suspension, it is 5.8 that lemon acid for adjusting pH value, which is added, in 37 DEG C of isoperibol
Middle enzymolysis 90min, obtains the first enzymolysis liquid, and it is 8 that wherein mixed enzyme, which is weight part ratio,:3:5:1 clostridiopetidase A, lipase, sugar
First enzyme and nuclease mix enzyme system；It keeps temperature and pH value constant, is added into the first enzymolysis liquid and the parts by weight such as mixed enzyme
Proteolytic enzyme, digest 120min after obtain the second enzymolysis liquid；
3) after the second enzymolysis liquid being detached, concentrated and dried, ox bone collagen protein peptide powder is obtained；
4) by ox bone collagen protein peptide powder and Chinese medical extract with weight part ratio 25:1 ratio, which is uniformly mixed, to be obtained
Ox bone collagen protein peptides；Wherein, the preparation method of the Chinese medical extract includes the following steps：
A, rhizome of davallia 20kg, Herba Epimedii 5kg, ginseng 10kg, root of Aucklandia lappa Decne 5kg, fruit of Chinese wolfberry 10kg, cordyceps sinensis 15kg, flea are taken
Not 5kg, Gorgon fruit 5kg, dark plum 8kg, fruit of Cherokee rose 12kg, arisaema cum bile 6kg, rhizome of cyrtomium 5kg, nine Ns of power 10kg, umbellate pore furgus perfume (or spice) 4kg and summer are withered
Ball 2kg dries 30h, and dry powder is made in crushing；
B, dry powder made from step a is added in Chinese medicine extracting tank and presses 1 with deionized water:20 ratio is uniformly mixed,
Stirring heats 6h, filters to obtain the first filtrate；
C, the filter residue in the first filtrate is collected, by 1:Deionized water is added in 8 ratio, stirs, and 3h is heated under the conditions of 60 DEG C,
The second filtrate is filtered to obtain, then the second filtrate is added in the first filtrate, is stirred, during distillation makes volume-diminished be obtained to 1/5
Medicine concentrate；
D, the concentrated liquid of tradition Chinese medicine made from step c is placed in fermentation tank, is added in fermentation tank and the bodies such as the concentrated liquid of tradition Chinese medicine
Long-pending deionized water is sterilized after stirring evenly, and the alpha-amylase of 0.2kg is added, and is stirred 1h under the conditions of 55 DEG C, is cooled to 50
DEG C, the pectase of 0.2kg is added, stirs 2h, obtains zymotic fluid；
E, Chinese medical extract is obtained after being extracted, chromatographed, eluted, be concentrated under reduced pressure and being dried to zymotic fluid.
Embodiment 2
The ox bone collagen protein peptides of embodiment 2 are to be uniformly mixed to be prepared by following component：Ox bone collagen protein peptide powder
25kg and Chinese medical extract 1kg.
3 electuary of embodiment
Embodiment 3 provides a kind of electuary, and the electuary is by following parts by weight at being grouped as：Ox bone made from embodiment 2
Collagen peptide 60.75g, beta-cyclodextrin 200g, steviol glycoside 20g, citric acid 5g, sesame pollen 10g, seabed coconut 8g.
4 capsule of embodiment
Embodiment 4 provides a kind of capsule, and the capsule is by following parts by weight at being grouped as：Made from embodiment 2
Ox bone collagen protein peptides 2.83g, corn Ruan 4g, vanillic aldehyde 1g, polacrilin potassium 2g, lactose 4g, heparin sodium 1g and citric acid fat
Fatty acid glyceride 5g.
The preparation method of the capsule is specific as follows：
A, ox bone collagen protein peptides are prepared；
B, corn Ruan of parts by weight is crushed to average grain diameter with micronizer and is less than 100 μm, volume fraction, which is added, is
90% ethanol water dissolves corn Ruan 5 times, and it is 2 times of weight of corn Ruan that ethanol water is added every time, obtains corn Ruan Yi
Alcoholic solution；
C, ox bone collagen protein peptides made from step a are taken to be added in Wet mixed granulating machine, setting mixing speed 600r/
Min shreds speed 1200r/min, mixes 15min, then corn Ruan ethanol solution made from step b is added to wet-mixing
Softwood is crossed 15 mesh and sieves wet granular processed by softwood processed in grain machine, and dry 90min, takes out under the conditions of wet granular is positioned over 50 DEG C, uses
15 mesh sieves, obtain hybrid particles；
D, vanillic aldehyde, polacrilin potassium, lactose, heparin sodium and the citric acid fatty glyceride and step c of parts by weight are taken
Hybrid particles obtained are uniformly mixed；It is packed into capsule shells to get capsule according to the loading amount of 0.25 ± 10%/grain.
5 capsule of embodiment
Embodiment 5 provides a kind of capsule, and the capsule is by following parts by weight at being grouped as：Made from embodiment 2
Ox bone collagen protein peptides 5.25g, corn Ruan 7g, vanillic aldehyde 2g, polacrilin potassium 3.5g, lactose 7g, heparin sodium 2g and citric acid
Fatty glyceride 10g.
The preparation method and embodiment 4 of the capsule are identical.
6 capsule of embodiment
Embodiment 6 provides a kind of capsule, and the capsule is by following parts by weight at being grouped as：Made from embodiment 1
Ox bone collagen protein peptides 8.17g, corn Ruan 10g, vanillic aldehyde 3g, polacrilin potassium 5g, lactose 10g, heparin sodium 3g and citric acid
Fatty glyceride 15g.
The preparation method and embodiment 4 of the capsule are identical.
Reference examples 1
Reference examples 1 provide a kind of preparation method of the ox bone collagen protein peptides with pre- preventing bone rarefaction effect, the ox
Chinese medicinal component in bone collagen peptide preparation method in Chinese medical extract includes：Rhizome of davallia 20kg, Herba Epimedii 5kg, ginseng
10kg, root of Aucklandia lappa Decne 5kg, fruit of Chinese wolfberry 10kg, cordyceps sinensis 15kg, paris polyphylla 5kg, Gorgon fruit 5kg, dark plum 8kg, tsaoko 12kg, arisaema cum bile
6kg, rhizome of cyrtomium 5kg, nine Ns of power 10kg, umbellate pore furgus perfume (or spice) 4kg and summer dry ball 2kg.
Other preparation process are identical with embodiment 1, and ox bone collagen protein peptides are made.
Reference examples 2
Reference examples 2 provide a kind of preparation method of the ox bone collagen protein peptides with pre- preventing bone rarefaction effect, the ox
Chinese medicinal component in bone collagen peptide preparation method in Chinese medical extract includes：Rhizome of davallia 20kg, Herba Epimedii 5kg, ginseng
10kg, root of Aucklandia lappa Decne 5kg, fruit of Chinese wolfberry 10kg, cordyceps sinensis 15kg, Radix Angelicae Sinensis 5kg, Gorgon fruit 5kg, dark plum 8kg, fruit of Cherokee rose 12kg, courage south
Star 6kg, rhizome of cyrtomium 5kg, nine Ns of power 10kg, umbellate pore furgus perfume (or spice) 4kg and summer dry ball 2kg.
Other preparation process are identical with embodiment 1, and ox bone collagen protein peptides are made.
Reference examples 3
Reference examples 3 provide a kind of preparation method of the ox bone collagen protein peptides with pre- preventing bone rarefaction effect, the ox
Chinese medicinal component in bone collagen peptide preparation method in Chinese medical extract includes：Rhizome of davallia 20kg, Schisandra chinensis 5kg, ginseng
10kg, root of Aucklandia lappa Decne 5kg, fruit of Chinese wolfberry 10kg, cordyceps sinensis 15kg, paris polyphylla 5kg, Gorgon fruit 5kg, dark plum 8kg, fruit of Cherokee rose 12kg, courage south
Star 6kg, rhizome of cyrtomium 5kg, nine Ns of power 10kg, umbellate pore furgus perfume (or spice) 4kg and summer dry ball 2kg.
Other preparation process are identical with embodiment 1, and ox bone collagen protein peptides are made.
4 electuary of reference examples
Reference examples 4 provide a kind of electuary, and the electuary is by following parts by weight at being grouped as：Ox bone made from embodiment 2
Collagen peptide 60.75g, beta-cyclodextrin 200g, steviol glycoside 20g, citric acid 5g, rape pollen 10g, seabed coconut 8g.
5 capsule of reference examples
Reference examples 5 provide a kind of capsule, and the capsule is by following parts by weight at being grouped as：
Ox bone collagen protein peptides 5.25g made from embodiment 2, corn Ruan 7g, vanillic aldehyde 2g, polacrilin potassium 3.5g, breast
Sugared 7g, polyvinylpyrrolidone 2g and citric acid fatty glyceride 10g.
The preparation method and embodiment 7 of the capsule are identical.
6 capsule of reference examples
Reference examples 6 provide a kind of capsule, and the capsule is by following parts by weight at being grouped as：
Ox bone collagen protein peptides 5.25g made from embodiment 2, corn Ruan 7g, vanillic aldehyde 2g, polacrilin potassium 3.5g, breast
Sugared 7g, heparin sodium 2g and polyethylene glycol monolaurate 10g.
The preparation method and embodiment 7 of the capsule are identical.
Improve osteoporosis effect analysis
1, crowd is tested
300 subjects, are clinical diagnosis sufferers of osteoporosis face, are randomly divided into test group 1,1 group of control and control 2
5 groups of group, 3 groups of control, 4 groups of control and control, every group of 50 people.
2, therapeutic scheme
Test group takes the ox bone collagen protein peptides described in the embodiment of the present invention 2, and three times a day, each 5g, half is small before meals
When take；Compare 1 group of ox bone collagen protein peptides taken described in reference examples 1, three times a day, each 5g, before meals half an hour take；
Compare 2 groups of ox bone collagen protein peptides taken described in reference examples 2, three times a day, each 5g, before meals half an hour take；Compare 3 groups
Take the ox bone collagen protein peptides described in reference examples 3, three times a day, each 5g, before meals half an hour take；4 groups are compareed to take
Ancient Chinese medical classics allusion quotation ancient prescription bone-strengthening powder, three times a day, each 5g, before meals half an hour take；It compares 5 groups and takes number of patent application and be
A kind of 201711407030.4 drug for treating osteoporosis, three times a day, each 5g；Whole subjects continuously take 15
It, refuses to obey Chinese and Western medicine or health products with any other treatment osteoporosis during treatment.
3, efficacy assessment standard
It cures：Osteoporosis symptoms completely disappear, and bone density is restored to normal range (NR)；
It is effective：Osteoporosis symptoms are substantially reduced before relatively treating, and bone density is significantly promoted but do not restored to normal range (NR)；
Effectively：Osteoporosis symptoms mitigate before relatively treating, and bone density is promoted but do not restored to normal range (NR) by a small margin；
In vain：Osteoporosis symptoms symptom is not improved performance, or has the tendency that exacerbation.
4, observation of curative effect
After taking composition 30 days, test group 1 cures 38, and cure rate 76% is effective 10, effective 1, total effective
Rate is 98%；Compare 1 group of healing 20, cure rate 46%, effective 6, effective 6, total effective rate 64%；Compare 2 groups
Healing 22, cure rate 44%, effective 3, effective 4, total effective rate 58%；3 groups of healings 18 are compareed, cure rate is
36%, effective 6, effective 10, total effective rate 68%；4 groups of healings 10 are compareed, cure rate 20%, has by effective 6
Effect 4, total effective rate 40%；Compare 5 groups of healings 10, cure rate 20%, effective 10, effective 4, total effective rate is
48%.
5, conclusion
The above results show that the ox bone collagen protein peptides of the application have effects that mental-tranquilization, dredging collateral wind-dispelling, have very well
Hypnotic effect, and user does not have any adverse reaction, and the crowd by insomnia puzzlement is suitble to take.
Hydroscopicity is tested
The powder of Example 2 and reference examples 4 respectively, is positioned over natural cooling in drier, the sample after drying is put
It is placed in the weighing cup with cover after drying, records the weight of weighing cup and sample, the weighing cup for filling drying sample is positioned over
In moisture maintainer, keep 60% constant humidity condition, respectively 2,4,6,8, sample is weighed afterwards for 24 hours, and calculate hydroscopicity.
As a result such as table 1.
Hydroscopicity (%)=100 (Wa-Wb)/Wb × 100%；
Wherein, Wa is the quality before sample is placed, and Wb is the quality after sample is placed.
The hydroscopicity test result of 1 inventive samples of table
As shown in Table 1, the hydroscopicity of embodiment 1 is significantly lower than reference examples 4, it was demonstrated that the present invention in powder by being added
The mixture of acrylic resin IV, low-substituted hydroxypropyl cellulose, magnesium trisilicate, xylitol, behenic acid glyceryl ester, Ke Yixian
The hygroscopicity of the reduction powder provided by the invention of work, prevents its moisture absorption hardened, when deletion or replaces any of which ingredient,
The hygroscopicity of powder provided by the invention can be improved.
Content range pattern
The capsule of Example 4-6 and each 10 parts of the capsule of reference examples 7-8, according to version in 2015《Chinese Pharmacopoeia》In
Method measure the uniformity of dosage units of its content, the results are shown in Table 2.
The uniformity of dosage units testing result of 2 capsule of the present invention of table
| Group | Uniformity of dosage units |
| Embodiment 4 | 97.31±2.34 |
| Embodiment 5 | 100.64±3.65 |
| Embodiment 6 | 99.32±3.13 |
| Reference examples 5 | 88.57±6.22 |
| Reference examples 6 | 86.46±4.31 |
As can be seen from Table 2, the content for the capsule that 4-5 of the embodiment of the present invention is provided has good content uniform
Degree, it was demonstrated that when addition corn Ruan, vanillic aldehyde, polacrilin potassium, lactose, heparin sodium and citric acid fatty acid glycerine in capsule
When the mixture of ester, can significantly improve the uniformity of dosage units of capsule, when to auxiliary material provided by the invention carry out delete or
When replacement, the uniformity of dosage units of capsule obtained is not inconsistent standardization, the uniformity of dosage units of content in the capsule of embodiment 5
It is apparently higher than embodiment 4 and embodiment 6, it was demonstrated that the preparation method of capsule provided by the invention can effectively improve capsule
The uniformity of dosage units of agent content.
The present invention provides a kind of ox bone collagen protein peptides preparation method with pre- preventing bone rarefaction effect, technique is first
Into, process is reasonable, operability is strong, is easy to industrialized production, ox bone collagen protein peptides prepared by this method have zhuanggu, tonifying Qi
The Chinese medicinal component of the effect of blood, tonifying spleen and stomach, kidney tonifying, strengthening healthy qi, addition can be cooperateed with ox bone collagen protein peptides, more be held
It easily absorbs, effect is far superior to the effect superposition of single medicinal material, can increase human bone mineral density, is suitble to patient with bone disease, bone damage
The wounded or bone density loss crowd take for a long time, reduce calcium loss, enhance bone density, also have conditioning chronic disease, improve life
The effect of quality.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
With within principle, any modification, equivalent substitution, improvement and etc. done should be included within the scope of protection of the invention god.
Claims (5)
1. a kind of preparation method of the ox bone collagen protein peptides with pre- preventing bone rarefaction effect, which is characterized in that include at least
Following steps：
1) ox bone after fresh clean is chosen, is placed it in cooking liquor and the boiling 3h at temperature under 150 DEG C of boiling temperature,
Then cooling 15min at a temperature of the ox bone after boiling being pulled out from cooking liquor and being placed in -80 DEG C while hot, with broken after taking-up
Ox bone is crushed the bovine bone powder for 50 mesh by machine, and bovine bone powder is rejoined in cooking liquor and obtains bovine bone powder suspension；Wherein, described
Cooking liquor is the mixed solution that solvent is water, and the concentration of each solute is respectively：Sodium hydroxide 80mM, sodium chloride 50mM, amino sulphur
Sour sodium 10mM, lactose 10mM；
2) mixed enzyme is added into bovine bone powder suspension, it is 5.8 that lemon acid for adjusting pH value, which is added, the enzyme in 37 DEG C of isoperibol
90min is solved, obtains the first enzymolysis liquid, it is 8 that wherein mixed enzyme, which is weight part ratio,:3:5:1 clostridiopetidase A, lipase, glycogen enzyme
Enzyme system is mixed with nuclease；It keeps temperature and pH value constant, the egg with parts by weight such as mixed enzymes is added into the first enzymolysis liquid
White hydrolase obtains the second enzymolysis liquid after digesting 120min；
3) after the second enzymolysis liquid being detached, concentrated and dried, ox bone collagen protein peptide powder is obtained；
4) by ox bone collagen protein peptide powder and Chinese medical extract with weight part ratio 25:1 ratio, which is uniformly mixed, obtains ox bone
Collagen peptide；Wherein, the preparation method of the Chinese medical extract includes the following steps：
A, 20 parts of the rhizome of davallia, 5 parts of Herba Epimedii, 10 parts of ginseng, 5 parts of root of Aucklandia lappa Decne, 10 parts of the fruit of Chinese wolfberry, 15 parts of cordyceps sinensis, paris polyphylla 5 are taken
Part, 5 parts of Gorgon fruit, 8 parts of dark plum, 12 parts of the fruit of Cherokee rose, 6 parts of arisaema cum bile, 5 parts, nine Ns 10 parts of power of rhizome of cyrtomium, umbellate pore furgus fragrant 4 parts and summer dry ball 2
Dry powder is made in part drying 30h, crushing；
B, dry powder made from step a is added in Chinese medicine extracting tank and presses 1 with deionized water:20 ratio is uniformly mixed, stirring,
6h is heated, the first filtrate is filtered to obtain；
C, the filter residue in the first filtrate is collected, by 1:Deionized water is added in 8 ratio, stirs, and 3h is heated under the conditions of 60 DEG C, filters
The second filtrate, then by the second filtrate be added the first filtrate in, be stirred, distillation make volume-diminished to 1/5 Chinese medicine it is dense
Contracting liquid；
D, the concentrated liquid of tradition Chinese medicine made from step c is placed in fermentation tank, is added in fermentation tank and the concentrated liquid of tradition Chinese medicine is isometric
Deionized water is sterilized after stirring evenly, and 0.2 part of alpha-amylase is added, stirs 1h under the conditions of 55 DEG C, is cooled to 50 DEG C,
0.2 part of pectase is added, stirs 2h, obtains zymotic fluid；
E, Chinese medical extract is obtained after being extracted, chromatographed, eluted, be concentrated under reduced pressure and being dried to zymotic fluid.
2. as described in claim 1 with pre- preventing bone rarefaction effect ox bone collagen protein peptides with it is pharmaceutically acceptable auxiliary
Electuary made of material, which is characterized in that further include auxiliary material, the weight part ratio of the ox bone collagen protein peptides and auxiliary material is 1:4,
The auxiliary material includes the ingredient of following parts by weight：200 parts of beta-cyclodextrin, 20 parts of steviol glycoside, 5 parts of citric acid, sesame pollen
10 parts, 8 parts of seabed coconut.
3. as described in claim 1 with pre- preventing bone rarefaction effect ox bone collagen protein peptides with it is pharmaceutically acceptable auxiliary
Capsule made of material, which is characterized in that further include auxiliary material, the weight part ratio of the ox bone collagen protein peptides and auxiliary material is 1:
6, the auxiliary material includes the ingredient of following parts by weight：4-10 parts of corn Ruan, 1-3 parts of vanillic aldehyde, 2-5 parts of polacrilin potassium, breast
5-15 parts of 4-10 parts sugared, 1-3 parts of heparin sodium and citric acid fatty glyceride.
4. capsule as claimed in claim 3, the capsule is prepared by the following method：
A, ox bone collagen protein peptides are prepared；
B, corn Ruan of parts by weight is crushed to average grain diameter with micronizer and is less than 100 μm, it is 90% that volume fraction, which is added,
Ethanol water by corn Ruan dissolve 5 times, every time be added ethanol water be 2 times of weight of corn Ruan, it is molten to obtain corn Ruan's ethyl alcohol
Liquid；
C, it takes ox bone collagen protein peptides made from step a to be added in Wet mixed granulating machine, sets mixing speed 600r/min,
Speed 1200r/min is shredded, mixes 15min, then corn Ruan ethanol solution made from step b is added to Wet mixed granulating machine
In softwood processed, softwood is crossed into 15 mesh and sieves wet granular processed, dry 90min, takes out, with 15 mesh under the conditions of wet granular is positioned over 50 DEG C
Whole grain is sieved, hybrid particles are obtained；
D, vanillic aldehyde, polacrilin potassium, lactose, heparin sodium and the citric acid fatty glyceride and step c for taking parts by weight are made
Hybrid particles be uniformly mixed；It is packed into capsule shells to get capsule according to the loading amount of 0.25 ± 10%/grain.
5. application of the ox bone collagen protein peptides described in claim 1 in preparing the product for pre- preventing bone rarefaction.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109744543A (en) * | 2019-01-09 | 2019-05-14 | 长沙市惠瑞生物科技有限公司 | A kind of bone collagen peptide composite powder and preparation method thereof |
| CN110693035A (en) * | 2019-11-01 | 2020-01-17 | 辽宁太爱肽生物工程技术有限公司 | Peptide-containing composition for osteoporosis people to eat and application thereof |
| CN112656937A (en) * | 2020-12-11 | 2021-04-16 | 广州天启生物科技有限公司 | Bovine bone collagen peptide composition and preparation method and application thereof |
| CN114028535A (en) * | 2021-12-16 | 2022-02-11 | 重庆希尔安药业有限公司 | Selenium-rich earthworm protein composition for promoting fracture healing and preparation method thereof |
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2018
- 2018-06-21 CN CN201810645123.9A patent/CN108770989A/en not_active Withdrawn
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109744543A (en) * | 2019-01-09 | 2019-05-14 | 长沙市惠瑞生物科技有限公司 | A kind of bone collagen peptide composite powder and preparation method thereof |
| CN110693035A (en) * | 2019-11-01 | 2020-01-17 | 辽宁太爱肽生物工程技术有限公司 | Peptide-containing composition for osteoporosis people to eat and application thereof |
| CN112656937A (en) * | 2020-12-11 | 2021-04-16 | 广州天启生物科技有限公司 | Bovine bone collagen peptide composition and preparation method and application thereof |
| CN112656937B (en) * | 2020-12-11 | 2021-07-20 | 广州天启生物科技有限公司 | Bovine bone collagen peptide composition and preparation method and application thereof |
| CN114028535A (en) * | 2021-12-16 | 2022-02-11 | 重庆希尔安药业有限公司 | Selenium-rich earthworm protein composition for promoting fracture healing and preparation method thereof |
| CN114028535B (en) * | 2021-12-16 | 2023-12-15 | 重庆希尔安药业有限公司 | Selenium-enriched earthworm protein composition for promoting fracture healing and preparation method thereof |
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