CN105111277A - Preparation method of ginseng peptide - Google Patents

Abstract

A preparation method of ginseng peptide uses ginseng to prepare ginseng peptide extract by means of multi-enzyme hydrolysis and includes: performing superfine grinding, performing sieving with a 100- to 400-mesh sifter; adding distilled water, and adjusting PH with citric acid and lactic acid to allow low-temperature treatment for 10-15 min; charging ginseng pulp into a test tube, plugging the test tube, putting the test tube in an ultrasonic water bath, pouring a product into an enzymatic vessel, allowing enzymatic hydrolysis, after enzymatic hydrolysis, performing filtering, and performing spray-drying to obtain powder. The ginseng peptide provided by the invention is simple to extract, operating is easy, and the ginseng peptide is available for batch production.

Description

A kind of preparation method of ginseng peptide

Technical field

The invention belongs to field of biological pharmacy, particularly relating to a kind of is starting material with ginseng, utilizes multienzyme enzymolysis making ginseng to be extracted into the method for part.

Background technology

Polypeptide is the compounds of molecular structure between amino acid and protein, it is by Amino acid profile, based on nutritive substance, polypeptide is easier than amino acid to be absorbed, and bioavailability is high, some low molecular peptide class, there is diseases prevention, cure the disease, regulate effect of human physiological functions, to human body, there is very important irreplaceable regulating effect, at present the research of polypeptide has been applied to the exploitation of medicine and heath food.Therefore,

Our company provides the extracting method of ginseng peptide.

Summary of the invention

The invention provides a kind of preparation method of ginseng peptide, take ginseng as raw material, adopts the method for multienzyme enzymolysis to make ginseng peptide and extracts composition.

To achieve these goals, the invention provides following technical scheme:

The preparation method of ginseng peptide, its step is as follows:

A, by being cut into the sheet of 5 mm of thickness after ginseng wash clean, carrying out micronizing, crossing 100 ~ 400 order medicines sieves;

After B, ratio in 1: 8 ~ 1: 12 add distilled water, regulate pH value to 3.6 ~ 4.8 with citric acid and lactic acid;

Under C, ginseng pulp steps A obtained are placed in the temperature of 30 ~ 45 DEG C, subzero treatment 10 ~ 15 minutes;

D, the ginseng pulp obtained by step C are placed in test tube, put into ultrasound bath after jumping a queue, thermostatic ultrasonic 30min;

E, pour in enzymatic vessel by the ginseng pulp of step D gained, adding activity is 55 ~ 85U/ml lignin-degrading enzymes, 50 ~ 60 DEG C of enzymolysis 0.7 ~ 1.8h;

F, add complex enzyme hydrolysis liquid in the ratios of 300 ~ 350 grams/ton, 35 DEG C, enzymolysis 72 hours, within every 4 hours, stir, oxygenation once, control pH value between 3.5 ~ 6.2;

After G, enzymolysis terminate, filter, residue adds 65 ~ 80% alcohol steep 2 ~ 3 times, and filter, merging filtrate, carries out spraying dry, and drying temperature is no more than 65 DEG C, and moisture control, below 7%, obtains powder.

By implementing above technical scheme, there is following technique effect: the simple and convenient extraction of ginseng peptide provided by the invention, easy to operate, can produce in batches.

Embodiment

For the method described in summary of the invention, provide embodiments of the invention further.

The embodiment of the present invention provides a kind of preparation method of ginseng peptide, and its step is as follows:

A, by being cut into the sheet of 5 mm of thickness after ginseng wash clean, carrying out micronizing, crossing 100 ~ 400 order medicines sieves;

After B, ratio in 1: 8 ~ 1: 12 add distilled water, regulate pH value to 3.6 ~ 4.8 with citric acid and lactic acid;

Under C, ginseng pulp steps A obtained are placed in the temperature of 30 ~ 45 DEG C, subzero treatment 10 ~ 15 minutes;

D, the ginseng pulp obtained by step C are placed in test tube, put into ultrasound bath after jumping a queue, thermostatic ultrasonic 30min;

E, pour in enzymatic vessel by the ginseng pulp of step D gained, adding activity is 55 ~ 85U/ml lignin-degrading enzymes, 50 ~ 60 DEG C of enzymolysis 0.7 ~ 1.8h;

F, add complex enzyme hydrolysis liquid in the ratios of 300 ~ 350 grams/ton, 35 DEG C, enzymolysis 72 hours, within every 4 hours, stir, oxygenation once, control pH value between 3.5 ~ 6.2;

After G, enzymolysis terminate, filter, residue adds 65 ~ 80% alcohol steep 2 ~ 3 times, and filter, merging filtrate, carries out spraying dry, and drying temperature is no more than 65 DEG C, and moisture control, below 7%, obtains powder.

Claims (1)

1. ginseng peptide and preparation method thereof, is characterized in that:

Preparation process is as follows:

A, by being cut into the sheet of 5 mm of thickness after ginseng wash clean, carrying out micronizing, crossing 100 ~ 400 order medicines sieves;

After B, ratio in 1: 8 ~ 1: 12 add distilled water, regulate pH value to 3.6 ~ 4.8 with citric acid and lactic acid;

Under C, ginseng pulp steps A obtained are placed in the temperature of 30 ~ 45 DEG C, subzero treatment 10 ~ 15 minutes;

D, the ginseng pulp obtained by step C are placed in test tube, put into ultrasound bath after jumping a queue, thermostatic ultrasonic 30min;

E, pour in enzymatic vessel by the ginseng pulp of step D gained, adding activity is 55 ~ 85U/ml lignin-degrading enzymes, 50 ~ 60 DEG C of enzymolysis 0.7 ~ 1.8h;

F, add complex enzyme hydrolysis liquid in the ratios of 300 ~ 350 grams/ton, 35 DEG C, enzymolysis 72 hours, within every 4 hours, stir, oxygenation once, control pH value between 3.5 ~ 6.2;

After G, enzymolysis terminate, filter, residue adds 65 ~ 80% alcohol steep 2 ~ 3 times, and filter, merging filtrate, carries out spraying dry, and drying temperature is no more than 65 DEG C, and moisture control, below 7%, obtains powder.