CN102094060A - Method for producing pressure reduction peptide from soybean as raw material - Google Patents
Method for producing pressure reduction peptide from soybean as raw material Download PDFInfo
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- CN102094060A CN102094060A CN 201010556397 CN201010556397A CN102094060A CN 102094060 A CN102094060 A CN 102094060A CN 201010556397 CN201010556397 CN 201010556397 CN 201010556397 A CN201010556397 A CN 201010556397A CN 102094060 A CN102094060 A CN 102094060A
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Abstract
The invention relates to a method for producing pressure reduction peptide from soybean as raw material, comprising the following steps of: mixing cooked soybean and water at the feed liquid ratio of 1:4-1:6;carrying out supersonic treatment for 20-60 minutes at the temperature of 40-65 DEG C; then processing under microwave for 1-3 minutes; cooling to 40-50 DEG C; adding 0.7-0.9%(w/w) of mixed enzyme preparation of lipase and protease into the feed liquid; when the pH value is 4.0-7.0, carrying out enzymolysis for 20-50 minutes; filtering the feed liquid subjected to enzymolysis, adding protease, and carrying out enzymolysis for 20-40 minutes at the temperature of 40-50DEG C when the pH value is 5.0-8.0; carrying out ultrafiltration to the feed liquid by a 4-8kDa ultrafiltration membrane at the temperature of 40 DEG C and the pressure of 0.1-0.2MPa for 30-50min; naking the ultrafiltration permeate respectively penetrate through H+ cation exchange resin and OH- anion exchange resin; collecting effluent, i.e. the pressure reduction peptide, wherein the ACE inhibition ratio of the pressure reduction peptide produced from the soybean is 87.3%.
Description
Technical field
The present invention relates to a kind of method of production step-down peptide, particularly relating to a kind of is the method for raw material production step-down peptide with the soybean.
Background technology
Hypertension is a kind of common cardiovascular disorder, its sickness rate height, often with the functional or organic change of heart, blood vessel, lung and kidney and other organs, be to cause various complication such as the heart, brain, kidney and eye blood vessel and cause apoplexy, promote the significant risk factor of atherosclerosis, coronary heart disease.The hypertension incidence rate reaches more than 20% in developed country, and the average attack rate of countries in the world is 10%-20%, and the crowd distributes extensively, comprises that the elderly, middle-aged people and children whole world every year reach 1,200 ten thousand because of the number of hypertension death.China had once carried out nationwide hypertension census three times, and the sickness rate of nineteen fifty-nine is that 5.11%, 1979～1980 annual morbidities are that 7.73%, 1990～1991 annual morbidities are 11.26%.At present, the existing hyperpietic of China has surpassed 1.2 hundred million populations.Therefore, treatment and preventing hypertension disease are current social crucial problems.
Hypertension generally has two types: essential hypertension and insecondary hypertension, the former mainly be since around the arteriole external caliber diminish or blood viscosity increases, cause Peripheral resistance too high due to; And Q volume of blood and kinemicly increase, the filling degree of the stiff degree of blood vessel and blood vessel all influences the variation of blood pressure; Autonomous nervous system also is an important factor to the regulating effect of blood pressure simultaneously.Insecondary hypertension also is symptomatic hypertension, is elevation of blood pressure concurrent in some lysis, and it is unusual etc. that its origin cause of formation mainly contains incretopathy, kidney disease, heart function.
(angiotensin converting enzyme. ACE) is a kind of zinciferous pepx to angiotensin-converting enzyme, can be activated and have the substrate specificity of broad by chlorion.ACE extensively is distributed in the mammiferous tissue.Renin-angiotensin system (renin-angiotensin system, RAS) and kallikrein one kinin system (kallikrein-kinin system, KKS) in, ACE plays important regulatory role to body blood pressure and cardiovascular function.The activity that suppresses ACE is considered to treat hypertensive a kind of important and effective means.At present, efficient, special in a large number ACE inhibitor is being brought into play important effect in treatment essential hypertension and congestive heart failure.
The food proteins source is abundant, conveniently is easy to get, and produces lower, safe, the suitable scale operation of active skin cost with food proteins.The step-down peptide that derives from food only plays the effect of step-down to the hyperpietic, the normotensive is not had hypotensive effect, can not produce step-down over-drastic problem; These peptides make under mild conditions with the enzyme of food grade, and its security is high, has no side effect; Except the function that step-down is arranged, also have effects such as anti-oxidant, digestion promoting, hypoglycemic, anti-platelet aggregation, enhancing body immunity simultaneously; Have higher thermostability, advantage such as water-soluble, can be used as functional factor and add to and go in beverage, the food, market outlook are fabulous.
Summary of the invention
The purpose of this invention is to provide a kind of is the method for raw material production step-down peptide with the soybean, and in this way preparing with the soybean is that the step-down peptide ACE inhibiting rate of raw material production can reach 87.3%.
For achieving the above object, the present invention adopts following technical scheme: a kind of is the method for raw material production step-down peptide with the soybean, it is characterized in that, well-done soybean was mixed with solid-liquid ratio with water in 1: 4～1: 6,40 ℃～65 ℃ of temperature, supersound process is 20～60 minutes under ultrasonic power 200W～400W condition; Under microwave 200W～500W condition, handled 1～3 minute again, be cooled to 40 ℃～50 ℃, (ratio of lipase and proteolytic enzyme is 3: 1～1: 3, and enzymolysis is 20～50 minutes under pH4.0～7.0 conditions to add the lipase of 0.7% (w/w)～0.9% (w/w) and the mixing enzyme preparation of proteolytic enzyme in the feed liquid.After feed liquid behind the enzymolysis is filtered, the proteolytic enzyme that adds 0.8% (w/w)～1.2% (w/w) in the filtrate, 40 ℃～50 ℃, enzymolysis is 20～40 minutes under pH5.0～8.0 conditions, feed liquid uses the ultra-filtration membrane of 8kDa～4kDa in 40 ℃, 0.1MPa～0.2MPa is to feed liquid ultrafiltration 30min～50min, ultrafiltration sees through liquid and passes through H more respectively
+Zeo-karb and OH
-Anionite-exchange resin.The effluent liquid of collecting be the ACE inhibiting rate can reach 87.3% be the step-down peptide of raw material production with the soybean.
Well-done soybean pulverizing was mixed with solid-liquid ratio with water in 1: 4～1: 6,40 ℃～65 ℃ of temperature, supersound process is 20～60 minutes under ultrasonic power 200W～400W condition;
Feed liquid after the supersound process was handled 1～3 minute under microwave 200W～500W condition again, was cooled to 40 ℃～50 ℃.
(ratio of lipase and proteolytic enzyme is 3: 1～1: 3, and enzymolysis is 20～50 minutes under pH4.0～7.0 conditions to add the lipase of 0.7% (w/w)～0.9% (w/w) and the mixing enzyme preparation of proteolytic enzyme in the feed liquid of cooling back.
After feed liquid behind the enzymolysis is filtered, add the proteolytic enzyme of 0.8% (w/w)～1.2% (w/w) in the filtrate, 40 ℃～50 ℃, enzymolysis is 20～40 minutes under pH5.0～8.0 conditions.
Feed liquid uses the ultra-filtration membrane of 8kDa～4kDa in 40 ℃, and 0.1MPa～0.2MPa is to feed liquid ultrafiltration 30min～50min, and ultrafiltration sees through liquid and passes through H more respectively
+Zeo-karb and OH
-Anionite-exchange resin.The effluent liquid of collecting.
Embodiment
Embodiment 1: well-done soybean mixed with solid-liquid ratio with water at 1: 4, and 40 ℃ of temperature, supersound process is 20 minutes under the ultrasonic power 200W condition; Handled 1 minute under microwave 500W condition, be cooled to 40 ℃, add the lipase of 0.7% (w/w) and the mixing enzyme preparation of proteolytic enzyme (ratio of lipase and proteolytic enzyme is 3: 1) in the feed liquid, enzymolysis is 30 minutes under the pH5.0 condition.After feed liquid behind the enzymolysis is filtered, add the proteolytic enzyme of 0.8% (w/w) in the filtrate, 50 ℃, enzymolysis is 20 minutes under the pH5.0 condition, and feed liquid uses the ultra-filtration membrane of 4kDa in 40 ℃, and 0.2MPa is to feed liquid ultrafiltration 50min, and ultrafiltration sees through liquid and passes through H more respectively
+Zeo-karb and OH
-Anionite-exchange resin.The effluent liquid of collecting
Embodiment 2: well-done soybean mixed with solid-liquid ratio with water at 1: 5, and 65 ℃ of temperature, supersound process is 30 minutes under the ultrasonic power 300W condition; Handled 2 minutes under microwave 300W condition, be cooled to 40 ℃, add the lipase of 0.8% (w/w) and the mixing enzyme preparation of proteolytic enzyme (ratio of lipase and proteolytic enzyme is 1: 1) in the feed liquid, enzymolysis is 40 minutes under the pH6.0 condition.After feed liquid behind the enzymolysis is filtered, add the proteolytic enzyme of 1.2% (w/w) in the filtrate, 45 ℃, enzymolysis is 30 minutes under the pH6.0 condition, and feed liquid uses the ultra-filtration membrane of 6kDa in 40 ℃, and 0.1MPa is to feed liquid ultrafiltration 40min, and ultrafiltration sees through liquid and passes through H more respectively
+Zeo-karb and OH
-Anionite-exchange resin.The effluent liquid of collecting.
Embodiment 3: well-done soybean mixed with solid-liquid ratio with water at 1: 6, and 55 ℃ of temperature, supersound process is 40 minutes under the ultrasonic power 400W condition; Handled 3 minutes under microwave 200W condition, be cooled to 50 ℃, add the lipase of 0.9% (w/w) and the mixing enzyme preparation of proteolytic enzyme (ratio of lipase and proteolytic enzyme is 1: 2) in the feed liquid, enzymolysis is 20 minutes under the pH4.0 condition.After feed liquid behind the enzymolysis is filtered, add the proteolytic enzyme of 1.0% (w/w) in the filtrate, 40 ℃～50 ℃, enzymolysis is 20 minutes under the pH5.5 condition, and feed liquid uses the ultra-filtration membrane of 8kDa in 40 ℃, and 0.2MPa is to feed liquid ultrafiltration 30min, and ultrafiltration sees through liquid and passes through H more respectively
+Zeo-karb and OH
-Anionite-exchange resin.The effluent liquid of collecting.
Claims (6)
1. one kind is the method for raw material production step-down peptide with the soybean, it is characterized in that, well-done soybean was mixed with solid-liquid ratio with water in 1: 4～1: 6, and 40 ℃～65 ℃ of temperature, supersound process is 20～60 minutes under ultrasonic power 200W～400W condition; Under microwave 200W～500W condition, handled 1～3 minute again, be cooled to 40 ℃～50 ℃, (ratio of lipase and proteolytic enzyme is 3: 1～1: 3, and enzymolysis is 20～50 minutes under pH4.0～7.0 conditions to add the lipase of 0.7% (w/w)～0.9% (w/w) and the mixing enzyme preparation of proteolytic enzyme in the feed liquid.After feed liquid behind the enzymolysis is filtered, the proteolytic enzyme that adds 0.8% (w/w)～1.2% (w/w) in the filtrate, 40 ℃～50 ℃, enzymolysis is 20～40 minutes under pH5.0～8.0 conditions, feed liquid uses the ultra-filtration membrane of 8kDa～4kDa in 40 ℃, 0.1MPa～0.2MPa is to feed liquid ultrafiltration 30min～50min, ultrafiltration sees through liquid and passes through H more respectively
+Zeo-karb and OH
-Anionite-exchange resin.The effluent liquid of collecting be the ACE inhibiting rate can reach 87.3% be the step-down peptide of raw material production with the soybean.
2. preparation method as claimed in claim 1 is characterized in that, well-done soybean pulverized mixed in 1: 4～1: 6 with solid-liquid ratio with water, and 40 ℃～65 ℃ of temperature, supersound process is 20～60 minutes under ultrasonic power 200W～400W condition.
3. the feed liquid after the supersound process was handled 1～3 minute under microwave 200W～500W condition again, was cooled to 40 ℃～50 ℃.
4. preparation method as claimed in claim 1, it is characterized in that, add the lipase of 0.7% (w/w)～0.9% (w/w) and the mixing enzyme preparation of proteolytic enzyme (ratio of lipase and proteolytic enzyme is 3: 1～1: 3) in the feed liquid of cooling back, enzymolysis is 20～50 minutes under pH4.0～7.0 conditions.
5. preparation method as claimed in claim 1 is characterized in that, after the feed liquid behind the enzymolysis is filtered, adds the proteolytic enzyme of 0.8% (w/w)～1.2% (w/w) in the filtrate, and 40 ℃～50 ℃, enzymolysis is 20～40 minutes under pH5.0～8.0 conditions.
6. preparation method as claimed in claim 1 is characterized in that, feed liquid uses the ultra-filtration membrane of 8kDa～4kDa in 40 ℃, and 0.1MPa～0.2MPa is to feed liquid ultrafiltration 30min～50min, and ultrafiltration sees through liquid and passes through H more respectively
+Zeo-karb and OH
-Anionite-exchange resin.The effluent liquid of collecting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201010556397 CN102094060A (en) | 2010-11-24 | 2010-11-24 | Method for producing pressure reduction peptide from soybean as raw material |
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| CN 201010556397 CN102094060A (en) | 2010-11-24 | 2010-11-24 | Method for producing pressure reduction peptide from soybean as raw material |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102265933A (en) * | 2011-08-12 | 2011-12-07 | 九阳股份有限公司 | Method for processing soybean milk raw materials and process for preparing soybean milk |
| CN104131056A (en) * | 2014-06-18 | 2014-11-05 | 江苏大学 | Sesame cake ACE inhibitory peptide preparation method based on microwave and ultrasonic wave technology and application |
| CN104480171A (en) * | 2014-11-21 | 2015-04-01 | 南宁知本康业生物技术有限公司 | Method for extracting polypeptide from velvet bean residue |
| CN105349240A (en) * | 2015-11-17 | 2016-02-24 | 江苏俊启粮油股份有限公司 | Method for extracting oil and protein hydrolysate in soybean |
| CN105420321A (en) * | 2015-12-01 | 2016-03-23 | 辽宁三合酒业有限公司 | Preparation method of weakly-alkaline soybean peptide |
| CN106434815A (en) * | 2016-12-05 | 2017-02-22 | 东北农业大学 | Method for preparing soybean antihypertensive peptides by membrane separation-electrodialysis technology |
| CN108208309A (en) * | 2017-12-28 | 2018-06-29 | 保龄宝生物股份有限公司 | The production method of low ash content soybean peptide containing profitable probliotics |
| CN109337950A (en) * | 2018-09-27 | 2019-02-15 | 山东禹王生态食业有限公司 | Protein content and the method for preparing soybean peptide in a kind of reduction bean dregs |
-
2010
- 2010-11-24 CN CN 201010556397 patent/CN102094060A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 《华侨大学学报( 自然科学版)》 20100531 刘静 等 蛋白酶解大豆多肽的理化特性 302-306 1-6 第31卷, 第3期 * |
| 《食品科学》 20071231 范远景 等 大豆蛋白酶解肽的分子量分布及抑制ACE 活性关系研究 57-61 1-6 第28卷, 第10期 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102265933A (en) * | 2011-08-12 | 2011-12-07 | 九阳股份有限公司 | Method for processing soybean milk raw materials and process for preparing soybean milk |
| CN104131056A (en) * | 2014-06-18 | 2014-11-05 | 江苏大学 | Sesame cake ACE inhibitory peptide preparation method based on microwave and ultrasonic wave technology and application |
| CN104480171A (en) * | 2014-11-21 | 2015-04-01 | 南宁知本康业生物技术有限公司 | Method for extracting polypeptide from velvet bean residue |
| CN105349240A (en) * | 2015-11-17 | 2016-02-24 | 江苏俊启粮油股份有限公司 | Method for extracting oil and protein hydrolysate in soybean |
| CN105420321A (en) * | 2015-12-01 | 2016-03-23 | 辽宁三合酒业有限公司 | Preparation method of weakly-alkaline soybean peptide |
| CN106434815A (en) * | 2016-12-05 | 2017-02-22 | 东北农业大学 | Method for preparing soybean antihypertensive peptides by membrane separation-electrodialysis technology |
| CN108208309A (en) * | 2017-12-28 | 2018-06-29 | 保龄宝生物股份有限公司 | The production method of low ash content soybean peptide containing profitable probliotics |
| CN109337950A (en) * | 2018-09-27 | 2019-02-15 | 山东禹王生态食业有限公司 | Protein content and the method for preparing soybean peptide in a kind of reduction bean dregs |
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