CN105349240A - Method for extracting oil and protein hydrolysate in soybean - Google Patents
Method for extracting oil and protein hydrolysate in soybean Download PDFInfo
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- CN105349240A CN105349240A CN201510790483.4A CN201510790483A CN105349240A CN 105349240 A CN105349240 A CN 105349240A CN 201510790483 A CN201510790483 A CN 201510790483A CN 105349240 A CN105349240 A CN 105349240A
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- Prior art keywords
- oil
- soybean
- protolysate
- protein hydrolysate
- emulsion
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
A provided method for extracting oil and protein hydrolysate in soybean comprises the following steps: 1) crushing soybean in a crusher, and then performing high-pressure steaming on the soybean powder; 2) mixing the material subjected to high-pressure steaming with water, adding a compound enzyme solution, and performing enzymolysis extraction on the mixed solution under an alkaline condition; 3) centrifuging the reaction product, so as to obtain free oil, an emulsion, a protein hydrolysate solution and residue; 4) performing microwave demulsification on the emulsion, so as to further obtain high-quality soybean oil; and 5) performing concentration, homogenization and spray drying on the protein hydrolysate solution, so as to obtain protein hydrolysate. According to the method, the soybean oil extraction rate is improved through high-temperature steaming processing, oil and protein are efficiently separated by using a microwave demulsification technology at a subsequent step, the oil extraction rate is further improved, and also protein hydrolysate is further separated.
Description
Technical field
The present invention relates to a kind of method extracting oil and protolysate in soybean.
Background technology
Traditionally, the extracting method of soybean oil is generally through fragmentation, softens, after rolling embryo, expelling-expansion pretreatment, mechanical expression or solvent extraction method is adopted to extract thick oil, concise rear acquisition again, although lixiviation process grease extraction efficiency higher (95%-98%), the wet dregs of rice easy sex change of albumen in high temperature desolvation process, and the use of organic solvent add technique triviality, reduce production security, cause environmental pollution.Mechanical expression method grease extraction efficiency is low, and protein denaturation is serious.
Aqueous enzymatic method uses new technique comparatively widely in recent years, it is in Mechanical Crushing ground foundation that aqueous enzymatic method carries oil tech, enzyme (comprising cellulase, polygalacturonase, amylase, proteolytic enzyme etc.) is adopted to act on oil plant, grease is made to be easy to discharge from oil plant solid, utilize non-oil component (albumen and carbohydrate) to oil and the avidity difference of water, utilize profit proportion different and by oil and non-oil component separating simultaneously.It carries oil tech as a kind of emerging " green, environmental protection ", can reclaim other value components in oil plant while extraction grease efficiently.But aqueous enzymatic method liquefaction is only confined to high oil bearing seed, and certain restriction is also had for low oil-containing oil plant soybean, and due to the existence of the materials such as the both sexes macromolecular substance protein in soybean, the aqueous enzymatic method being rich in oil/water is carried in oil systems and inevitably forms o/w type milk sap, this is not only separated to system and brings great challenge, and seriously limit oily yield, and protein extracting ratio is also not too high, causes protein resource to waste to a certain extent.
Summary of the invention
In order to solve after the lower and enzymolysis of oil yield in soybean leaching process the easily not segregative problem of emulsification, the invention provides a kind of method extracting oil and protolysate in soybean, comprising the following steps:
1) soybean is pulverized in pulverizer, then soyflour is carried out steaming and decocting under high pressure; Steaming and decocting under high pressure can make that cell walls is loose, starch pasting, increases perviousness, is beneficial to the process of follow-up prozyme, soybean oil is more easily discharged;
2) material after steaming and decocting under high pressure is mixed with water, add composite enzyme solution, in the basic conditions enzymolysis and extraction is carried out to mixed solution;
3) reacted product is carried out centrifugal, obtain free oil, emulsion, Hydrolyzed protein liquid and residue;
4) microwave demulsifying is carried out to emulsion, the soybean oil of high-quality can be obtained further;
5) concentrated by Hydrolyzed protein liquid, homogeneous, spraying dry can obtain hydrolytic soya bean protein;
Described step 1) pressure of mesohigh boiling is 0.4-0.8Mpa.
Described step 1) time of mesohigh boiling is 60-240min.
Described step 2) in complex enzyme liquid comprise Sumizyme MP, alkali cellulose enzyme and alkaline pectase.
Described step 4) in time of microwave demulsifying be 10-30min.
The present invention has following beneficial effect:
The present invention by high temperature steaming pre-treatment, can improve the extraction yield of soybean oil, and in the technique of follow-up use microwave demulsifying, can separating oil and protein efficiently, both can improve oily extraction yield further, also can time protolysate be separated further.
Embodiment
Embodiments of the invention are described below.The element described in one embodiment of the invention and feature can combine with the element shown in one or more other embodiment and feature.It should be noted that for purposes of clarity, in explanation, eliminate expression and the description of unrelated to the invention, parts known to persons of ordinary skill in the art and process.
Embodiment 1
The present embodiment provides a kind of method extracting oil and protolysate in soybean, is below concrete implementation process:
1) soybean is pulverized in medicinal herb grinder, then by soyflour boiling 60min when 0.4Mpa;
2) mixed with water by the material after steaming and decocting under high pressure, add Sumizyme MP, alkali cellulose enzyme and alkaline pectase, under agitation carry out enzymolysis and extraction, extraction time is 5h;
3) product after enzymolysis and extraction is carried out centrifugal, obtain free oil, emulsion, Hydrolyzed protein liquid and residue;
4) carry out microwave demulsifying to emulsion, the microwave demulsifying time is 10min;
5) concentrated by Hydrolyzed protein liquid, homogeneous, spraying dry can obtain hydrolytic soya bean protein.
Through measuring and calculating, the percent of total oil of the present embodiment is 93.4%, and hydrolytic soya bean protein extraction yield is 95.5%.
Embodiment 2
The present embodiment provides a kind of method extracting oil and protolysate in soybean, is below concrete implementation process:
1) soybean is pulverized in medicinal herb grinder, then by soyflour boiling 120min when 0.6Mpa;
2) mixed with water by the material after steaming and decocting under high pressure, add Sumizyme MP, alkali cellulose enzyme and alkaline pectase, under agitation carry out enzymolysis and extraction, extraction time is 3h;
3) product after enzymolysis and extraction is carried out centrifugal, obtain free oil, emulsion, Hydrolyzed protein liquid and residue;
4) carry out microwave demulsifying to emulsion, the microwave demulsifying time is 20min;
5) concentrated by Hydrolyzed protein liquid, homogeneous, spraying dry can obtain hydrolytic soya bean protein.
Through detecting, the percent of total oil of the present embodiment is 96.3%, and hydrolytic soya bean protein extraction yield is 97.2%.
Embodiment 3
The present embodiment provides a kind of method extracting oil and protolysate in soybean, is below concrete implementation process:
1) soybean is pulverized in medicinal herb grinder, then by soyflour boiling 240min when 0.8Mpa;
2) mixed with water by the material after steaming and decocting under high pressure, add Sumizyme MP, alkali cellulose enzyme and alkaline pectase, under agitation carry out enzymolysis and extraction, extraction time is 8h;
3) product after enzymolysis and extraction is carried out centrifugal, obtain free oil, emulsion, Hydrolyzed protein liquid and residue;
4) carry out microwave demulsifying to emulsion, the microwave demulsifying time is 30min;
5) concentrated by Hydrolyzed protein liquid, homogeneous, spraying dry can obtain hydrolytic soya bean protein.
Through calculating, the percent of total oil of the present embodiment is 93.4%, and hydrolytic soya bean protein extraction yield is 94.2%.
As can be seen from the above embodiments, adopt this extracting method, percent of total oil is more than 90%, the yield of hydrolytic soya bean protein is more than 94%, this is because after introducing high temperature steaming, can softening of the cell wall be made, broken, alkalescence prozyme more easily acts on cell walls, make the grease stripping in cell, adopt microwave treatment, heat effect and hole effect can destroy the combination of macro-molecular protein and grease, protolysate is more easily extracted, so this technology has significant progress.
Although last it is noted that described the present invention and advantage thereof in detail above, be to be understood that and can carry out various change when not exceeding the spirit and scope of the present invention limited by appended claim, substituting and converting.And scope of the present invention is not limited only to the specific embodiment of process, equipment, means, method and step described by specification sheets.One of ordinary skilled in the art will readily appreciate that from disclosure of the present invention, can use perform the function substantially identical with corresponding embodiment described herein or obtain and its substantially identical result, existing and that will be developed in the future process, equipment, means, method or step according to the present invention.Therefore, appended claim is intended to comprise such process, equipment, means, method or step in their scope.
Claims (5)
1. extract a method for oil and protolysate in soybean, it is characterized in that, comprise the following steps:
1) soybean is pulverized in pulverizer, then soyflour is carried out steaming and decocting under high pressure;
2) material after steaming and decocting under high pressure is mixed with water, add composite enzyme solution, in the basic conditions enzymolysis and extraction is carried out to mixed solution;
3) reacted product is carried out centrifugal, obtain free oil, emulsion, Hydrolyzed protein liquid and residue;
4) microwave demulsifying is carried out to emulsion, obtain the soybean oil of high-quality further;
5) concentrated by Hydrolyzed protein liquid, homogeneous, spraying dry can obtain hydrolytic soya bean protein.
2. a kind of method extracting in soybean oil and protolysate according to claim 1, is characterized in that, described step 1) pressure of mesohigh boiling is 0.4-0.8Mpa.
3. a kind of method extracting in soybean oil and protolysate according to claim 1, is characterized in that, described step 1) time of mesohigh boiling is 60-240min.
4. a kind of method extracting in soybean oil and protolysate according to claim 1, is characterized in that, described step 2) middle complex enzyme liquid comprises Sumizyme MP, alkali cellulose enzyme and alkaline pectase.
5. a kind of method extracting in soybean oil and protolysate according to claim 1, is characterized in that, described step 4) time of middle microwave demulsifying is 10-30min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510790483.4A CN105349240A (en) | 2015-11-17 | 2015-11-17 | Method for extracting oil and protein hydrolysate in soybean |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510790483.4A CN105349240A (en) | 2015-11-17 | 2015-11-17 | Method for extracting oil and protein hydrolysate in soybean |
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| CN105349240A true CN105349240A (en) | 2016-02-24 |
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| CN201510790483.4A Pending CN105349240A (en) | 2015-11-17 | 2015-11-17 | Method for extracting oil and protein hydrolysate in soybean |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107047781A (en) * | 2017-04-19 | 2017-08-18 | 南昌大学 | High added value soymilk prepared by a kind of profit fusion method |
Citations (7)
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|---|---|---|---|---|
| CN101608206A (en) * | 2009-06-01 | 2009-12-23 | 黑龙江省大豆技术开发研究中心 | Preparation method of soy protein immune-active peptides and products thereof |
| CN101974364A (en) * | 2009-09-27 | 2011-02-16 | 东北农业大学 | Method for extracting soybean grease from soybean emulsion |
| CN102094060A (en) * | 2010-11-24 | 2011-06-15 | 河南工业大学 | Method for producing pressure reduction peptide from soybean as raw material |
| CN102329844A (en) * | 2010-09-13 | 2012-01-25 | 东北农业大学 | Microbial fermentation enzymolysis method for extracting grease and protein of soybean |
| CN102911782A (en) * | 2012-11-13 | 2013-02-06 | 东北农业大学 | Demulsification method for aqueous enzymatic extraction of soybean oil and fat |
| CN103820217A (en) * | 2014-02-19 | 2014-05-28 | 董志新 | Water extraction method of oil by microwave disperse centrifugation |
| CN104357211A (en) * | 2014-10-29 | 2015-02-18 | 颍上县永祥旱粮研究所 | Processing method of soybean oil |
-
2015
- 2015-11-17 CN CN201510790483.4A patent/CN105349240A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101608206A (en) * | 2009-06-01 | 2009-12-23 | 黑龙江省大豆技术开发研究中心 | Preparation method of soy protein immune-active peptides and products thereof |
| CN101974364A (en) * | 2009-09-27 | 2011-02-16 | 东北农业大学 | Method for extracting soybean grease from soybean emulsion |
| CN102329844A (en) * | 2010-09-13 | 2012-01-25 | 东北农业大学 | Microbial fermentation enzymolysis method for extracting grease and protein of soybean |
| CN102094060A (en) * | 2010-11-24 | 2011-06-15 | 河南工业大学 | Method for producing pressure reduction peptide from soybean as raw material |
| CN102911782A (en) * | 2012-11-13 | 2013-02-06 | 东北农业大学 | Demulsification method for aqueous enzymatic extraction of soybean oil and fat |
| CN103820217A (en) * | 2014-02-19 | 2014-05-28 | 董志新 | Water extraction method of oil by microwave disperse centrifugation |
| CN104357211A (en) * | 2014-10-29 | 2015-02-18 | 颍上县永祥旱粮研究所 | Processing method of soybean oil |
Non-Patent Citations (3)
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| 李杨 等: "模糊评判优化水酶法提取膨化大豆油脂和蛋白", 《农业工程学报》 * |
| 杨慧萍 等: "高压蒸煮、超声波辅助水酶法处理米糠技术研究", 《粮食与饲料工业》 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107047781A (en) * | 2017-04-19 | 2017-08-18 | 南昌大学 | High added value soymilk prepared by a kind of profit fusion method |
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