CN102911782A - Demulsification method for aqueous enzymatic extraction of soybean oil and fat - Google Patents
Demulsification method for aqueous enzymatic extraction of soybean oil and fat Download PDFInfo
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- CN102911782A CN102911782A CN2012104531621A CN201210453162A CN102911782A CN 102911782 A CN102911782 A CN 102911782A CN 2012104531621 A CN2012104531621 A CN 2012104531621A CN 201210453162 A CN201210453162 A CN 201210453162A CN 102911782 A CN102911782 A CN 102911782A
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Abstract
The invention relates to a demulsification method for aqueous enzymatic extraction of soybean oil and fat, which belongs to the vegetable oil and fat extraction technology and comprises the steps as follows: crushing soybeans and performing extrusion preprocessing after moisture adjustment to obtain an extruded material; crushing the extruded material and mixing the extruded material with water after sieving to obtain a mixed liquor; adding alkaline protease into the mixed liquor to perform enzymolysis, and performing centrifugal separation after enzymolysis to obtain free oil, emulsion, hydrolysate and residue; adding ethanol into the emulsion, heating up to 60 DEG C after stirring, keeping for 30 min, and performing demulsification processing through a high intensity pulsed electric field; and performing centrifugal separation after the demulsification processing, so as to obtain the soybean oil and fat. The demulsification method has the advantages of simple required technology equipment, convenience for operation, high demulsification rate, and short time and high safety for demulsification.
Description
Technical field
The invention belongs to the extraction processing technology of Vegetable oil lipoprotein, relate generally to a kind of method of aqueous enzymatic extraction soybean oil breakdown of emulsion.
Background technology
Breakdown of emulsion is very crucial step in the aqueous enzymatic extraction soybean oil, and breakdown of emulsion is more thorough, and the rate of recovery of grease is higher.The quality of breakdown of emulsion directly has influence on the economic worth of whole technique.The formation of milk sap and stability thereof are two aspects of milk sap field primary study.In many cases, people wish that the milk sap for preparing can resist droplet coalescence, keep stable, but wish that sometimes milk sap is unstable, can breakdown of emulsion and make oily water separation.For the true emulsion that forms, destroying it, to reach the purpose that is separated be very difficult.Breaking method commonly used has physical mechanical method, physico-chemical processes and electric power effect three classes.The physical mechanical method has centrifugation, ultrasonication, heating etc.; Physico-chemical processes mainly is the character that changes the emulsion interfaces film by adding mineral acid, salt or polymeric flocculant etc., reaches the breakdown of emulsion purpose; The electric power effect is to utilize high-voltage potential to promote that charged drop is coalescent in the milk sap.One of advantage of aqueous enzymatic method technique is to avoid with an organic solvent and poisonous chemical reagent, for this reason for the milk sap that forms in the aqueous enzymatic extraction, adopts the physical mechanical method breakdown of emulsion the safest.Hanmoungjai (2001) adopts proteolytic enzyme to act on rice bran, and to the emulsion layer method breakdown of emulsion that boils that obtains, oil only is 79% and 68% with the rate of recovery of protein.Aparna (2002) adopts 18000g ultra-high speed centrifugation breakdown of emulsion, and oily yield has improved 40% than contrast, but this centrifugation apparatus can't be applied to scale operation.Moura etc. (2008) adopt proteolytic enzyme in conjunction with the method breakdown of emulsion of pH adjusting or Phospholipid hydrolase enzymolysis, the grease of 70%-80% in the recyclable milk sap in soybean aqueous enzymatic method oil-producing technique.Carry in the oil at the soybean aqueous enzymatic method, Wu (2009) explores the mode breakdown of emulsion of enzymolysis and regulation system pH, Jung (2009) further reclaims the free oil condition to the endo-protease breakdown of emulsion and is optimized research, to contributing for the development that advances this environmentally friendly oil extracting process of aqueous enzymatic method.In addition, the foreign scholar adopts organic solvent directly to extract oil in the milk sap under study for action mostly, with the oily effect that from the oil plant cell, discharges behind the investigation enzymolysis, and the research that further breaks through from technological layer does not appear in the newspapers, and, existing demulsification technology exists the breakdown of emulsion time long, and demulsification efficiency is low, the problems such as breakdown of emulsion poor stability.
Summary of the invention
Technical problem to be solved by this invention is to overcome above-mentioned the deficiencies in the prior art, and a kind of method of aqueous enzymatic extraction soybean oil breakdown of emulsion is provided, and reaches the purpose of simplifying demulsification technology, improving demulsification efficiency, safe breakdown of emulsion.
Technical problem to be solved by this invention is achieved through the following technical solutions:
A kind of method of aqueous enzymatic extraction soybean oil breakdown of emulsion, obtain expanded material with carrying out expelling-expansion pretreatment behind the soybean adjusting moisture after pulverizing, be mixed to get mixed solution with water again after crossing 60 orders behind the expanded crushing material, the mass ratio of described amount of water and expanded material is 6:1, add Sumizyme MP in the mixed solution and carry out enzymolysis 2.5h, behind the enzymolysis under 5000r/min centrifugation 30min obtain free oil, milk sap, hydrolyzed solution and residue; Add ethanol in the milk sap and stir 10min post-heating to 60 ℃, and keep 30min, carry out breakdown of emulsion through pulsed electric field again and process; Centrifugation 15min both must soybean oil under the 8000r/min rotating speed afterwards in the breakdown of emulsion processing.
Described pulse strength is 26-30 kV/cm, and the burst length is 160-380 μ s, and pulse velocity is 50-70mL/min, and pulse-repetition is 400-500Hz.
Present method adopts pulsed electric field to carry out breakdown of emulsion and processes, so that the emulsifying film of albumen and grease breaks, thereby so that grease is separated out in the milk sap, and then so that grease is released, have the advantages that required processing unit is simple, the breakdown of emulsion time is short, demulsification efficiency is high, breakdown of emulsion is safe.
Description of drawings
Accompanying drawing is overall process route figure of the present invention;
Embodiment
Below in conjunction with accompanying drawing the specific embodiment of the invention is described in detail.
A kind of method of aqueous enzymatic extraction soybean oil breakdown of emulsion, obtain expanded material with carrying out expelling-expansion pretreatment behind the soybean adjusting moisture after pulverizing, be mixed to get mixed solution with water again after crossing 60 orders behind the expanded crushing material, the mass ratio of described amount of water and expanded material is 6:1, add Sumizyme MP in the mixed solution and carry out enzymolysis 2.5h, behind the enzymolysis under 5000r/min centrifugation 30min obtain free oil, milk sap, hydrolyzed solution and residue; Add ethanol in the milk sap and stir 10min post-heating to 60 ℃, and keep 30min, carry out breakdown of emulsion through pulsed electric field again and process; Centrifugation 15min both must soybean oil under the 8000r/min rotating speed afterwards in the breakdown of emulsion processing.
Described pulse strength is 26-30 kV/cm, and the burst length is 160-380 μ s, and pulse velocity is 50-70mL/min, and pulse-repetition is 400-500Hz.
Embodiment 1:
Obtain expanded material with carrying out expelling-expansion pretreatment behind the adjusting moisture to 14% after the soybean pulverizing, 60 mesh sieves will be crossed behind the expanded crushing material, take by weighing the expanded material behind the 100g crushing screening, stir behind the adding 600ml water and obtain mixed solution, regulate pH of mixed to 9.5, add Sumizyme MP after heating to 60 ℃ and carry out enzymolysis, behind the enzymolysis 2.5h under the rotating speed of 5000r/min centrifugation 30min obtain free oil, milk sap, hydrolyzed solution and residue; Add volume fraction in the milk sap and be 85% ethanol and stir 10min, after milk sap is warming up to 60 ℃, keep 30min, pulse strength, pulse velocity at 28 kV/cm are 60mL/min, pulse-repetition be carry out under the 450Hz pulsed electric field process behind the 200s under 8000r/min centrifugation 15min both soybean oil, the demulsification efficiency of this moment is 92%.
Embodiment 2:
Obtain expanded material with carrying out expelling-expansion pretreatment behind the adjusting moisture to 14% after the soybean pulverizing, 60 mesh sieves will be crossed behind the expanded crushing material, take by weighing the expanded material behind the 100g crushing screening, stir behind the adding 600ml water and obtain mixed solution, regulate pH of mixed to 9.5, add Sumizyme MP after heating to 60 ℃ and carry out enzymolysis, behind the enzymolysis 2.5h under the rotating speed of 5000r/min centrifugation 30min obtain free oil, milk sap, hydrolyzed solution and residue; Add volume fraction in the milk sap and be 85% ethanol and stir 10min, after milk sap is warming up to 60 ℃, keep 30min, pulse strength, pulse velocity at 28 kV/cm are 80mL/min, pulse-repetition be carry out under the 500Hz pulsed electric field process behind the 200s under 8000r/min centrifugation 15min both soybean oil, the demulsification efficiency of this moment is 88%.
Embodiment 3:
Obtain expanded material with carrying out expelling-expansion pretreatment behind the adjusting moisture to 14% after the soybean pulverizing, 60 mesh sieves will be crossed behind the expanded crushing material, take by weighing the expanded material behind the 100g crushing screening, stir behind the adding 600ml water and obtain mixed solution, regulate pH of mixed to 9.5, add Sumizyme MP after heating to 60 ℃ and carry out enzymolysis, behind the enzymolysis 2.5h under the rotating speed of 5000r/min centrifugation 30min obtain free oil, milk sap, hydrolyzed solution and residue; Add volume fraction in the milk sap and be 85% ethanol and stir 10min, after milk sap is warming up to 60 ℃, keep 30min, pulse strength, pulse velocity at 28 kV/cm are 60mL/min, pulse-repetition be carry out under the 400Hz pulsed electric field process behind the 200s under 8000r/min centrifugation 15min both soybean oil, the demulsification efficiency of this moment is 84%.
Claims (2)
1. the method for an aqueous enzymatic extraction soybean oil breakdown of emulsion, it is characterized in that: carry out expelling-expansion pretreatment behind the soybean adjusting moisture after will pulverizing and obtain expanded material, be mixed to get mixed solution with water again after crossing 60 orders behind the expanded crushing material, the mass ratio of described amount of water and expanded material is 6:1, add Sumizyme MP in the mixed solution and carry out enzymolysis 2.5h, behind the enzymolysis under 5000r/min centrifugation 30min obtain free oil, milk sap, hydrolyzed solution and residue; Add ethanol in the milk sap and stir 10min post-heating to 60 ℃, and keep 30min, carry out breakdown of emulsion through pulsed electric field again and process; Centrifugation 15min both must soybean oil under the 8000r/min rotating speed afterwards in the breakdown of emulsion processing.
2. the method for a kind of aqueous enzymatic extraction soybean oil breakdown of emulsion according to claim 1, it is characterized in that: described pulse strength is 26-30 kV/cm, and the burst length is 160-380 μ s, and pulse velocity is 50-70mL/min, and pulse-repetition is 400-500Hz.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103571609A (en) * | 2013-11-14 | 2014-02-12 | 东北农业大学 | Method for synchronously preparing high-stability rice bran oil and rice bran protein |
| CN103589505A (en) * | 2013-11-29 | 2014-02-19 | 东北农业大学 | Evening primrose oil extraction method |
| CN104450174A (en) * | 2014-09-25 | 2015-03-25 | 华侨大学 | Demulsification method for tea seed oil aqueous enzymatic method |
| CN105349240A (en) * | 2015-11-17 | 2016-02-24 | 江苏俊启粮油股份有限公司 | Method for extracting oil and protein hydrolysate in soybean |
| CN106070660A (en) * | 2016-07-09 | 2016-11-09 | 东北农业大学 | A kind of method of aqueous enzymatic extraction Semen sojae atricolor nanometer emulsion |
| CN107434998A (en) * | 2017-08-29 | 2017-12-05 | 湖南源绿科技有限公司 | A kind of method that high-voltage pulse electric field coordination aqueous enzymatic method prepares camellia seed oil |
| CN109266432A (en) * | 2018-09-26 | 2019-01-25 | 江苏大学 | Method that is a kind of while extracting Silybum Marianum Gaertn Seed Oil and protein hydrolysate |
| CN115093895A (en) * | 2022-06-30 | 2022-09-23 | 江南大学 | Device and method for preparing rapeseed oil by aid of high-voltage alternating-current pulse electric field |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103571609A (en) * | 2013-11-14 | 2014-02-12 | 东北农业大学 | Method for synchronously preparing high-stability rice bran oil and rice bran protein |
| CN103589505A (en) * | 2013-11-29 | 2014-02-19 | 东北农业大学 | Evening primrose oil extraction method |
| CN104450174A (en) * | 2014-09-25 | 2015-03-25 | 华侨大学 | Demulsification method for tea seed oil aqueous enzymatic method |
| CN105349240A (en) * | 2015-11-17 | 2016-02-24 | 江苏俊启粮油股份有限公司 | Method for extracting oil and protein hydrolysate in soybean |
| CN106070660A (en) * | 2016-07-09 | 2016-11-09 | 东北农业大学 | A kind of method of aqueous enzymatic extraction Semen sojae atricolor nanometer emulsion |
| CN107434998A (en) * | 2017-08-29 | 2017-12-05 | 湖南源绿科技有限公司 | A kind of method that high-voltage pulse electric field coordination aqueous enzymatic method prepares camellia seed oil |
| CN109266432A (en) * | 2018-09-26 | 2019-01-25 | 江苏大学 | Method that is a kind of while extracting Silybum Marianum Gaertn Seed Oil and protein hydrolysate |
| CN115093895A (en) * | 2022-06-30 | 2022-09-23 | 江南大学 | Device and method for preparing rapeseed oil by aid of high-voltage alternating-current pulse electric field |
| CN115093895B (en) * | 2022-06-30 | 2023-10-20 | 江南大学 | Device and method for preparing rapeseed oil with assistance of high-voltage alternating-current pulsed electric field |
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Application publication date: 20130206 |