CN103636917B - Utilize Hydrodynamic Cavitation Technology from rice residue, extract the method for rice protein - Google Patents
Utilize Hydrodynamic Cavitation Technology from rice residue, extract the method for rice protein Download PDFInfo
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- CN103636917B CN103636917B CN201310694833.8A CN201310694833A CN103636917B CN 103636917 B CN103636917 B CN 103636917B CN 201310694833 A CN201310694833 A CN 201310694833A CN 103636917 B CN103636917 B CN 103636917B
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Abstract
The present invention relates to a kind of method utilizing Hydrodynamic Cavitation Technology to extract rice protein from rice residue, comprise the following steps: the dry rice residue of getting water content < 10%, add a certain amount of hot water and obtain rice residue slurry, then carry out pretreatment by equipment of Hydrodynamic Cavitation; Add a certain amount of Novi letter AMS and Novi letter composite plant hydrolase Viscozyme more respectively? L, by equipment of Hydrodynamic Cavitation circular treatment; Be warming up to 80 ~ 95 DEG C after process terminates and filter to obtain rice protein filter cake; Filter cake obtains rice protein through hot wash, filtration, drying, pulverizing.The present invention utilize Hydrodynamic cavitation can broken rice residue particle, discharge impurity, accelerate enzyme ' s reaction speeding, the soybean protein purity prepared can reach more than 85% simultaneously.
Description
Technical field
The present invention relates to a kind of extracting method of rice protein, particularly a kind of method utilizing Hydrodynamic Cavitation Technology to extract rice protein from rice residue.
Background technology
Rice residue take rice as the accessory substance that starch sugar produced by raw material, and rice residue remains the most of protein in rice, is the good raw material preparing rice protein.But due to the High-temperature Liquefaction process in starch sugar production process, make the protein in rice residue there occurs denaturation, and these protein are closely wrapped up by outer field starch, dextrin and oligosaccharide usually, cause rice protein to be difficult to be efficiently extracted by the rankine steam cycle out.
At present, the method extracting rice protein mainly contains alkali extraction-acid precipitation, protease method and amylase impurity removal method etc.Rice protein extracts by the principle that wherein alkali extraction-acid precipitation make use of the molten acid of protein alkali heavy, but this technique impels lysine in protein and alanine, cystine generation condensation reaction because using the aqueous slkali of higher concentration, generate poisonous compound, also can reduce the nutritive value of lysine simultaneously; In addition, this method protein recovery is lower.Protease method is, by protease, the proteolysis in rice residue is become soluble small-molecule substance, realizes the extraction of rice protein, and the product dissolubility that this method obtains is good, but the rate of recovery is also lower.Amylase impurity removal method adopts amylase the starch that remain in rice residue and dextrin to be removed, and to improve the content of protein, but the lower and protein content of product of existing technology production efficiency is difficult to more than 80%.
Summary of the invention
The technical problem to be solved in the present invention is: provide a kind of method utilizing Hydrodynamic Cavitation Technology to extract rice protein from rice residue, the method production efficiency is high, and Protein yield is high, and in product, protein content can reach more than 85%.
The technical scheme solved the problems of the technologies described above is: a kind of method utilizing Hydrodynamic Cavitation Technology to extract rice protein from rice residue, comprise the following steps: the dry rice residue of getting water content < 10%, adding dry rice residue quality 3 ~ 10 times of temperature is that the hot water of 50 ~ 55 DEG C obtains rice residue slurry, then carry out pretreatment by equipment of Hydrodynamic Cavitation, treatment conditions are: feed temperature 45 ~ 55 DEG C, outlet pressure 0.2-0.4MPa, circulation 2 ~ 5 times; Add Novi's letter AMS of dry rice residue quality 0.5-1.0 ‰ again, regulate pH to 5.8-6.2, then by rice residue slurry under the condition of temperature 55 ~ 60 DEG C, outlet pressure 0.1-0.2MPa, by equipment of Hydrodynamic Cavitation circular treatment 2 ~ 5 times; Add the Novi letter composite plant hydrolase ViscozymeL of dry rice residue quality 0.1-0.4% again, regulate pH to 4.8-5.2, then by rice residue slurry under the condition of temperature 55 ~ 60 DEG C, outlet pressure 0.1-0.2MPa, by equipment of Hydrodynamic Cavitation circular treatment 2 ~ 5 times; Rice protein filter cake is filtered to obtain after being warming up to 80 ~ 95 DEG C; Rice protein filter cake obtains rice protein through hot wash, filtration, drying, pulverizing; Described Novi's letter AMS is middle temperature 480L type, and activity is 450-500KNU/g, and the activity of described Novi letter composite plant hydrolase ViscozymeL is 80-120FBG/g.
Described equipment of Hydrodynamic Cavitation comprises with the high-order circulating tank of agitator, turbulence pump and heat exchanger, described high-order circulating tank, turbulence pump and heat exchanger pass through pipeline communication successively, described heat exchanger passes through pipeline communication with high-order circulating tank again, and described outlet pressure refers to turbulence pump outlet pressure.
Pipeline between described high-order circulating tank and turbulence pump is provided with charging control valve, pipeline between described turbulence pump and heat exchanger is provided with Pressure gauge and discharging control valve, the pipeline between described heat exchanger and high-order circulating tank is provided with discharging triple valve.
Described rice residue slurry, after Hydrodynamic cavitation process terminates, adopts injector to be warming up to 80 ~ 95 DEG C.
Hydrodynamic cavitation is a kind of new technology strengthening biochemical process, and its principle utilizes the bubble in fluid to produce the cavitation effects such as Local Instantaneous high temperature and high pressure, strong shock wave and microjet in moment of crumbling and fall to induce and the change of enhanced biological macromolecular complex reason, chemistry and structural property.The present invention utilizes Hydrodynamic Cavitation Technology to extract rice protein from rice residue, main technical principle comprises: (1) utilizes Hydrodynamic cavitation to carry out pretreatment to rice residue, rice residue particle is diminished, the inclusion enclave that protein simultaneously in destruction rice residue and starch, dextrin and oligosaccharide are formed, impurity beyond isolating protein is fully discharged, improves follow-up enzyme process impurity-eliminating effect; (2) utilize hydrodynamic cavitation to assist enzymatic hydrolysis, increase the interaction probability of enzyme-to-substrate, thus accelerate enzyme ' s reaction speeding.
The present invention is based on existing amylase impurity removal process, introduces Hydrodynamic Cavitation Technology and rice residue particle is diminished, destroys the inclusion enclave that protein in rice residue and starch, dextrin and oligosaccharide formed, the carrying out of promotion enzyme digestion reaction simultaneously; Also the composite plant hydrolase containing arabanase, cellulase, 1,4 beta-glucanase, hemicellulase and zytase etc. is used in dedoping step, to remove non-starch impurity further in addition.This technology production efficiency is high, Protein yield is high, protein content can reach more than 85% in product.
Below, in conjunction with the accompanying drawings and embodiments the technical characteristic utilizing Hydrodynamic Cavitation Technology to extract the method for rice protein from rice residue of the present invention is further described.
Accompanying drawing explanation
Fig. 1: the equipment of Hydrodynamic Cavitation structural representation of the present invention.
In figure: 1-high-order circulating tank, 11-agitator, 2-charging control valve, 3-turbulence pump, 4-Pressure gauge, 5-discharging control valve, 6-heat exchanger, 7-discharging triple valve.
Detailed description of the invention
Embodiment 1: a kind of method utilizing Hydrodynamic Cavitation Technology to extract rice protein from rice residue, comprises the following steps:
Get the dry rice residue 1kg of water content < 10%, adding dry rice residue quality 5 times of temperature is that the hot water of 50 ~ 55 DEG C obtains rice residue slurry, then puts into and is equipped with Ni Keni turbulence pump (power 0.4kw, flow 1.0m
3/ h) equipment of Hydrodynamic Cavitation under 0.3MPa pretreatment 15min, rice residue particle is diminished, destroys the inclusion enclave that wherein protein and starch, dextrin and oligosaccharide formed.
The concrete structure of described equipment of Hydrodynamic Cavitation is: comprise with the high-order circulating tank 1 of agitator 11, turbulence pump 3 and plate type heat exchanger 6, described high-order circulating tank, turbulence pump and heat exchanger are successively by pipeline communication, and described heat exchanger passes through pipeline communication with high-order circulating tank again; Pipeline between described high-order circulating tank and turbulence pump is provided with charging control valve 2, the pipeline between turbulence pump and heat exchanger is provided with Pressure gauge 4 and discharging control valve 5, the pipeline between heat exchanger and high-order circulating tank is provided with discharging triple valve 7.Feed liquid (rice residue slurry) is circulated in systems in which by the effect of turbulence pump, and temperature is controlled by plate type heat exchanger 6.
High-order circulating tank 1 first put into by described rice residue slurry, and restart turbulence pump 3 and carry out Hydrodynamic cavitation process, then return again in high-order circulating tank 1, circular treatment 15min(approximately circulates 5 times)
, in processing procedure, turbulence pump 3 export Stress control at 0.3MPa, feed temperature 50 DEG C.
After Hydrodynamic cavitation pretreatment terminates, then the Novi adding dry rice residue quality 1.0 ‰ (1g) believes that AMS (middle temperature 480L type, activity is 480KNU/g) enzymolysis is to remove starch, dextrin, with food-grade soda ash (Na
2cO
3) regulate pH to 6.0, under temperature 55 DEG C, turbulence pump outlet pressure 0.1MPa, circular treatment 15min(approximately circulates 5 times); Add dry rice residue quality 0.4%(4g again) Novi's letter composite plant hydrolase ViscozymeL(activity be 100FBG/g,) enzymolysis to be to remove the carbohydrate of non-starch, regulate pH to 5.0 with food grade hydrochloric acid (HCl), under temperature 55 DEG C, turbulence pump outlet pressure 0.1MPa, circular treatment 15min(approximately circulates 5 times); Through plate-frame filtering, washing, filtration, drying, pulverizing after being warming up to 95 DEG C with injector, gained rice protein content is more than 85%.
In the present embodiment, the thermal source of plate type heat exchanger and injector is steam.
One as the present embodiment converts, and can also adopt the equipment of Hydrodynamic Cavitation of other versions, as long as material can be made to form eddy current wherein produce Hydrodynamic cavitation effect.
Claims (3)
1. the method utilizing Hydrodynamic Cavitation Technology to extract rice protein from rice residue, it is characterized in that: comprise the following steps: the dry rice residue of getting water content < 10%, adding dry rice residue quality 3 ~ 10 times of temperature is that the hot water of 50 ~ 55 DEG C obtains rice residue slurry, then carry out pretreatment by equipment of Hydrodynamic Cavitation, treatment conditions are: feed temperature 45 ~ 55 DEG C, outlet pressure 0.2-0.4MPa, circulation 2 ~ 5 times; Add Novi's letter AMS of dry rice residue quality 0.5-1.0 ‰ again, regulate pH to 5.8-6.2, then by rice residue slurry under the condition of temperature 55 ~ 60 DEG C, outlet pressure 0.1-0.2MPa, by equipment of Hydrodynamic Cavitation circular treatment 2 ~ 5 times; Add the Novi letter composite plant hydrolase ViscozymeL of dry rice residue quality 0.1-0.4% again, regulate pH to 4.8-5.2, then by rice residue slurry under the condition of temperature 55 ~ 60 DEG C, outlet pressure 0.1-0.2MPa, by equipment of Hydrodynamic Cavitation circular treatment 2 ~ 5 times; Rice protein filter cake is filtered to obtain after being warming up to 80 ~ 95 DEG C; Rice protein filter cake obtains rice protein through hot wash, filtration, drying, pulverizing; Described Novi's letter AMS is middle temperature 480L type, and activity is 450-500KNU/g, and the activity of described Novi letter composite plant hydrolase ViscozymeL is 80-120FBG/g; Described equipment of Hydrodynamic Cavitation comprises with the high-order circulating tank (1) of agitator (11), turbulence pump (3) and heat exchanger (6), described high-order circulating tank, turbulence pump and heat exchanger pass through pipeline communication successively, described heat exchanger passes through pipeline communication with high-order circulating tank again, and described outlet pressure refers to turbulence pump outlet pressure.
2. the method utilizing Hydrodynamic Cavitation Technology to extract rice protein from rice residue according to claim 1, it is characterized in that: the pipeline between described high-order circulating tank and turbulence pump is provided with charging control valve (2), pipeline between described turbulence pump and heat exchanger is provided with Pressure gauge (4) and discharging control valve (5), the pipeline between described heat exchanger and high-order circulating tank is provided with discharging triple valve (7).
3. the method utilizing Hydrodynamic Cavitation Technology to extract rice protein from rice residue according to claim 1 and 2, is characterized in that: described rice residue slurry, after Hydrodynamic cavitation process terminates, adopts injector to be warming up to 80 ~ 95 DEG C.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103947822B (en) * | 2014-05-08 | 2015-11-04 | 无锡群硕谷唐生物科技有限公司 | A kind of CL rice protein liquid and preparation method thereof and its purposes |
| CN105177088A (en) * | 2015-07-31 | 2015-12-23 | 广西科开成林科技有限公司 | Method of using tapioca flour to cleanly prepare nitrogenous syrup |
| CN110496415B (en) * | 2019-07-31 | 2021-01-01 | 武汉大学 | Method for extracting anthocyanin by injection type cavitation, application and device used by method |
| CN111494283B (en) * | 2020-05-30 | 2020-12-25 | 欧露莲生物科技(广东)有限公司 | Garlic extract composition skin care product containing garlic peptide and preparation process thereof |
| CN116463167A (en) * | 2022-05-04 | 2023-07-21 | 新纪元食品科技(佛山)有限公司 | Puffing treatment process for complete material |
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| CN101869173A (en) * | 2010-07-06 | 2010-10-27 | 长沙高新开发区湘博医药科技有限公司 | Method for extracting rice protein powder by utilizing enzyme-alkali method |
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Patent Citations (3)
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| CN1557230A (en) * | 2004-01-29 | 2004-12-29 | 大连理工大学 | Hydraulic cavitate sterilizing apparatus |
| CN102481263A (en) * | 2009-07-07 | 2012-05-30 | 萨班哲大学 | Crosslinked protein nanocrystals, crosslinked protein nanoaggregates and method of preparation thereof |
| CN101869173A (en) * | 2010-07-06 | 2010-10-27 | 长沙高新开发区湘博医药科技有限公司 | Method for extracting rice protein powder by utilizing enzyme-alkali method |
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| Hydrodynamic Cavitation for Food and Water Processing;Parag R. Gogate;《Food Bioprocess Technol》;20111231(第4期);第996-1011页 * |
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