CN102807629A

CN102807629A - Method for extracting rice bran polysaccharides by using continuous countercurrent ultrasonic equipment

Info

Publication number: CN102807629A
Application number: CN2011101478924A
Authority: CN
Inventors: 陈希权
Original assignee: Hegang Sanjiang Pingyuan Rice Industry Group Co Ltd
Current assignee: Hegang Sanjiang Pingyuan Rice Industry Group Co Ltd
Priority date: 2011-06-01
Filing date: 2011-06-01
Publication date: 2012-12-05

Abstract

The invention provides a method for extracting rice bran polysaccharides by using continuous countercurrent ultrasonic equipment. An appropriate adding amount of an enzyme preparation, an appropriate PH value and an optimal control temperature during countercurrent continuous extraction are determined by selecting efficient high-temperature-resistant alpha-amylase and high-temperature-resistant glucoamylase. Due to the adoption of the method, the reaction time is shortened, the product yield is increased, and an obtained product has good quality; and bran residues left after extraction of the rice bran polysaccharides is used for producing a rice bran nutrient and rice bran dietary fiber, so that rice bran is fully utilized, waste residues are not discharged in a production process, bran residues in the production process can be utilized, and the comprehensive utilization way of rice bran is expanded.

Description

Utilize the continuous countercurrent ultrasonic equipment to extract the method for rice bran polysaccharide

Affiliated technical field

The invention belongs to the modern biotechnology category.

Technical background

In China, utilize fresh rice bran to extract rice bran polysaccharide, generally adopt hot water leaching, enzymolysis, ethanol precipitating method, this method adopts batch technology.Exist deficiency to be, low, the weak effect of selecting for use of zymin activity had both influenced quality product, also influenced the back one-step refining of product, and yield is not high, and the production cycle is longer, extracted the chaff slag of rice bran polysaccharide and failed to fully utilize, and product is single.

Summary of the invention

For deficiency and the problem that overcomes above-mentioned existence; The objective of the invention is to provide a kind of method of utilizing the continuous countercurrent ultrasonic equipment to extract rice bran polysaccharide; Select efficient high-temperature resistant AMS and high temperature resistant saccharifying enzyme for use, optimum control temperature when confirming suitable enzymes preparation add-on, suitable pH value and counter current continuous extraction.Both shortened the reaction times, and improved product yield again, the quality product of gained is better, with the chaff slag behind the extraction rice bran polysaccharide, produces rice bran nutrients and rice bran food fibre, reaches rice bran and all utilizes, and production process does not have waste residue and discharges.

The technical solution adopted for the present invention to solve the technical problems is to utilize continuous countercurrent ultrasonic equipment extraction rice bran polysaccharide, realization rice bran polysaccharide serialization production; Select high temperature high efficiency α-lytic enzyme and high temperature high efficiency saccharifying enzyme for use, through confirming rational operating parameters: pH value is 5.0, and temperature is controlled at 85 ℃; Best zymin addition, high temperature resistant AMS is 3,000U/kg; High temperature resistant saccharifying enzyme is 6,000U/kg.Rice bran polysaccharide yield＞90%, rice bran polysaccharide content＞95%; Make full use of the chaff slag that extracts behind the rice bran polysaccharide again, produce rice bran nutrients and rice bran food fibre.

The invention has the beneficial effects as follows, take both to have guaranteed quality product after the above-mentioned measures, can utilize the chaff slag of production process again, produce rice bran nutrients and rice bran food fibre, expanded the rice bran comprehensive Utilization Ways.

Description of drawings

Below in conjunction with accompanying drawing and embodiment the present invention is advanced an explanation.

Accompanying drawing 1 is a process flow sheet of the present invention.

Among the figure 1, the fresh rice bran of passivation, 2, continuous countercurrent ultrasonic extraction equipment, 3, rotary drum filter, 4, the enamel enzymatic vessel, 5, the enamel saccharifying tank, 6, centrifugal filter, 7, alcohol analyses jar, 8, the butterfly filter, 9, the cryodrying device, 10, rice bran polysaccharide; 11, the chaff slag that merges protein gelatin, 12, colloidal mill, 13, the butterfly centrifugal machine, 14, the wet cyclone group, 15, filtrating A storage tank; 16, enamel liquefaction, saccharifying tank group, 17, the rotary drum separating machine, 18, the liquor B storage tank, 19, filtrating A, B mixed solution storage tank; 20, falling film condenser group, 21, the drying machine with centrifugal spray group, 22, the screening and packing machine, 23, rice bran nutrients; 24, robust fibre filter cake, 25, drying machine, 26, vibratory screening apparatus and wrapping machine, 27, the rice bran food fibre.

Embodiment

(1) with after fresh rice bran passivation and the removal of impurities, get the fresh rice bran 1 of passivation, subsequent use;

(2) extraction: the fresh rice bran of passivation 1 is dropped in the continuous countercurrent ultrasonic extraction equipment 2, press mass ratio and 1: 10～15 add deionized waters, stir,, be heated to 85 ℃, continuous extraction under this temperature with 10% hydrochloric acid accent medium PH=5.0;

(3) press filtration: the extraction mixture send rotary drum filter 3, with the small amount of deionized water washing, collects the chaff slag and is used to produce rice bran nutrients and rice bran food fibre.Merge washings and filtrating, store in the lump;

(4) enzymolysis: filtrating is pumped into enamel liquefied pot 4, add high temperature resistant AMS (add-on is by 3, and 000U/kg estimates), stir, be warming up to 90 ℃, keep pH value 5.8～6.2, liquefied 1 hour, check negative to Iod R; Be cooled to 60 ℃, pump into and add saccharifying enzyme (add-on by 6,000U/kg estimation) in the saccharifying tank 5, regulate pH value 3.6～4.0, stirring reaction 1 hour; PH value=4.5 to proteins coagulation iso-electric point is regulated in cooling, separates out protein gel;

(5) centrifuging: mixed solution is sent in the centrifuge separator 6 after the saccharification, spinning, and the filter residue protein gelatin merges with extraction back chaff slag, collects filtrating;

(6) alcohol is analysed: filtrating is pumped into enamel alcohol analyse in jars 7, add 95% ethanol, make that ethanol content reaches more than 60% in the material, added ethanol by about 1: 1.7 and get final product, mix, separate out rice bran polysaccharide, and complete until precipitating;

(7) filtration drying: alcohol is analysed material send into dish formula filter 8, and use the small amount of ethanol washed twice, merging filtrate reclaims ethanol.Volatilize the alcoholic acid filter cake, send in the vacuum drier 9,, must contain the rice bran polysaccharide finished product 10 of sugared total amount more than 95% 40 °～60 ℃ dryings;

The protein gelatin that the chaff slag that (8) will extract rice bran polysaccharide is separated out when producing rice bran polysaccharide merges into 11; Suitably add deionized water, send into colloidal mill 12 defibrinations, mixed serum pumps in disk centrifugal separator 13 and the wet cyclone 14; Spinning; Tell filtrating A and send into storage tank 15, isolated chaff slag is delivered to enamel liquefaction, saccharifying tank group 16, add the liquefaction of high-temperature and saccharifying enzyme, saccharification;

(10) in liquefaction, saccharifying tank group 16, add AMS earlier, AMS is a kind of restriction endonuclease, can cut α-1,4 key in the starch molecule, but can not cut α-1,6 key.Under the effect of this kind of enzyme, starch is degraded into the bigger Fructus Hordei Germinatus oligose of SANMALT-S, trisaccharide maltose and molecular weight very soon in the rice bran.And disperse or dissolve, this process is called liquefaction, so that partial starch separates with Mierocrystalline cellulose.And then the adding saccharifying enzyme, saccharifying enzyme is also referred to as starch α-1, and the 4-glucuroide is a kind of excision enzyme.Can directly generate glucose from starch molecule non-reducing end hydrolyzing alpha successively-1,4 key.And glucose is water-soluble sugar, its starch in the rice bran of not only can degrading, and can also directly be produced as the glucose of absorption of human body, and increasing the oryzanal nutritive value, this stage parameter control is with producing the rice bran polysaccharide operation;

(11) filter: hydrolysis, saccharification liquid are sent in the rotary drum filter 17, got liquor B, be stored in storage tank 18; A merges with filtrating, sends into and mixes storage tank 19, will mix filtrating then and deliver to economic benefits and social benefits falling liquid film vacuum concentration machine group 20; Cryoconcentration; Liquid concentrator is delivered to vacuum spray drying device 21 dryings, and the finished product after the spraying oven dry promptly gets rice bran nutrients 23 through screening, wrapping machine 22;

(12) falling liquid film concentrates parameter control: rice bran nutrients belongs to thermo-sensitivity food, therefore need adopt cryogenic vacuum to concentrate and spraying drying, and use the air dielectric that purifies and sterilize.This process using economic benefits and social benefits falling liquid film vacuum concentrator; Not only energy-conservation; Also be suitable for thermo-sensitivity organic solution and concentrate, the falling-film evaporator group is mainly imitated with 2 effect heating fumigators, vapour separator at night, preheating sterilizer, vapor jet pump, hydraulic jet pump, storage tank etc. by 1 and is partly formed.Cold material utilizes secondary steam to be preheated to 40 °～50 ℃ through preheat coil earlier, gets into 2 and imitates preheating tubes, is preheating to 60 °～65 ℃; Imitate preheating tubes to 1 again, be preheating to 70 °～80 ℃, utilize steam heating to 86 °～96 ℃ at heating tube at last; And in pipeline, keep this temperature, after the sterilization of completion material, get into 1 single-effect evaporator top distribution plate; Through distributing the sieve plate uniform distribution; Be membranaceous dirty along heated wall, concentrate, the secondary steam of generation and liquid concentrator get into vapour liquid separator with tangential direction together.1 effect Heating temperature is 83 °～85 ℃, and temperature of charge is 70 °～72 ℃ in the pipe, and the warp spissated material first time is with being pumped into 2 single-effect evaporator top materials distribution sieve plate.The 1st imitates a secondary steam part of allotting supplies 2 effects to do heating steam usefulness, and another part supplies the vapo(u)r blasting pump as power.The vaporization temperature of the 2nd effect is lower than 1 imitates, and low approximately (15 °～20 ℃) reach more than 40% through twice spissated material concentration, get into storage tank, and liquid concentrator is delivered to the vacuum spray drying device.In this system, the secondary steam of the 2nd effect can be used for the preheating fresh material.Because of this equipment, heat energy can be recycled, and belongs to energy-saving equipment.Generally speaking, evaporation 1kg water only consumes the new steam of 0.44kg;

(13) filter residue of liquefy, saccharification liquid being told is sent into drying in the drying machine 25, sends into vibratory screening apparatus and packing unit 26 again, gets rice finished product bran dietary fiber 27.

Claims

1. a method of utilizing the continuous countercurrent ultrasonic equipment to extract rice bran polysaccharide is characterized in that selecting for use high temperature high efficiency α-lytic enzyme and high temperature high efficiency saccharifying enzyme, and through confirming rational operating parameters: pH value is 5.0; Temperature is controlled at 85 ℃; Best zymin addition, high temperature resistant AMS is 3,000U/kg; High temperature resistant saccharifying enzyme is 6,000U/kg.Rice bran polysaccharide (10) yield＞90%, rice bran polysaccharide (10) content＞95%; Make full use of the chaff slag that extracts behind the rice bran polysaccharide (10) again, produce rice bran nutrients (23) and rice bran food fibre (27).