CN102094059A - Method for preparing soybean polypeptide by microwave-promoted hydrolysis of soybean protein - Google Patents
Method for preparing soybean polypeptide by microwave-promoted hydrolysis of soybean protein Download PDFInfo
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- CN102094059A CN102094059A CN2009102731941A CN200910273194A CN102094059A CN 102094059 A CN102094059 A CN 102094059A CN 2009102731941 A CN2009102731941 A CN 2009102731941A CN 200910273194 A CN200910273194 A CN 200910273194A CN 102094059 A CN102094059 A CN 102094059A
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Abstract
The invention discloses a method for preparing soybean polypeptide by microwave-promoted hydrolysis of soybean protein, which mainly fills in the blank of preparing the soybean polypeptide from the soybean protein in a short time in China. In the method, the soybean protein is subjected to alkali solution and acid precipitation to be purified, and the soybean polypeptide is prepared by a method of combining a microwave technology with a biological enzymolysis technology. By the method, a polypeptide product which has good appearance, good taste, strong functions, short preparation time and low production cost is developed. The method has the prominent advantages that: the soybean polypeptide prepared by a microwave-promoted enzymolysis technology is superior to that prepared by the conventional enzymolysis or fermentation technology; the obtained product does not contain impurities, has small molecular weight and is more easily absorbed and used by human bodies; and the process is simple, the reaction period is short, the enzymolysis degree is easy to control, and the product has high quality and good effects.
Description
Technical field
The present invention relates to quicken the processing technology of protein polypeptide hydrolysis, specifically refer to promote soybean protein hydrolysis to prepare the method for soybean polypeptide with microwave.
Background technology
Soybean originates from China, is one of main farm crop of China, with the northeast soybean optimal quality.The soybean of countries in the world cultivation is all directly or indirectly blazed abroad by China.Because its nutritive value is very high, be called as " kings in the beans ", " Tanaka's meat ", " green cow's milk " etc., be subjected in hundreds of natural foods that the nutritionist praises highly most edible.Soybean contains rich in protein, contains 18 seed amino acids of human body needs, can be used as a kind of proteinaceous nutrient reinforcer and additive, alleviates the shortage of protein resource.Yet, development along with the soyabean processing industry, soybean protein is fully used substantially, and the soybean after the oil expression can be used as high-quality feed, and soybean protein can be made the nutritional auxiliary of higher protein contents such as soybean protein concentrate, soybean protein isolate through further processing.In these years along with the fast development of polypeptide industry, people recognize the function and the nutritive value of polypeptide more.Along with the appearance of first soybean polypeptide national standard, indicate that the polypeptide industry has formally entered the developmental stage of a standardization, ordering.Soybean polypeptide prepares in modes such as enzymolysis or fermentations usually, and relative cycle is longer, and the production cost height is mainly used in the protective foods.And soybean protein can be prepared in a short period of time soybean polypeptide with the short zymolysis technique of microwave, reaction times shortens to tens minutes, compared with conventional enzymolysis or fermentation technique need a few hours, the reaction time of tens hours even a couple of days, shortened the reaction times greatly, this technology market at home still belongs to blank.
Summary of the invention
Purpose of the present invention is exactly will provide a kind of to promote soybean protein hydrolysis to prepare the method for soybean polypeptide with microwave.The heat effect and the non-thermal effect of utilization microwave utilize the heat effect heating, use the short zymolysis technique of biological enzymolysis technology and microwave non-thermal effect that soybean polypeptide is prepared simultaneously.Adopt this method technology simple, the enzymolysis degree is easy to control, and gained soybean polypeptide purity height, effect are good.For achieving the above object, what the present invention was designed promotes soybean protein hydrolysis to prepare the method for soybean polypeptide with microwave, in turn includes the following steps:
1) take by weighing soybean protein 1000～2000g, carry out alkali extraction and acid precipitation and handle, cleaning is removed impurity and is obtained soybean protein isolate;
2) soybean protein isolate that obtains is put into microwave reactor, add in the distilled water or pure water of 2000～4000ml, stir, regulate its pH value to 7.0～9.0 with potassium hydroxide, place under the microwave condition to keep 10～30 minutes, microwave power 500～1000w carries out denaturing treatment to soybean protein isolate, cooling obtains the soybean protein isolate aqueous solution;
3) press 2～6% of soybean protein isolate raw material weight again, get Sumizyme MP, join step 2) in the soybean protein aqueous solution of gained, opening microwave generator makes temperature rise to 30～70 ℃ to carry out enzymolysis, microwave power 100-1000w, micro-wave energy continues supply during the enzymolysis, and the microwave enzymolysis time was kept 5～30 minutes, obtains the soybean protein isolate enzymolysis solution;
4) the soybean protein isolate enzymolysis solution microwave power with the step 3) gained is increased to 500～2000w, keep 2～10 minutes enzymic activitys of going out more than 90 ℃, it is cooled to 30～50 ℃ then, carry out micro-filtrate membrane filtration, remove filter residue, obtain soybean protein isolate enzymolysis filtrate;
5) activity charcoal powder that accounts for its volume 1%～3% will be added in the soybean protein isolate enzymolysis filtrate of step 4) gained, regulate its pH value to 3.0～7.0 with oxalic acid, keep that its temperature is decoloured under 30～80 ℃ of conditions, debitterize handled 1～3 hour, then it is filtered, remove the waste active carbon slag, obtain the smart filtrate of soybean polypeptide;
6) the smart filtrate of the soybean polypeptide of step 5) gained is carried out cryogenic vacuum concentrates, spray drying treatment obtains soyabean polypeptide powder.
Further, above-mentioned steps 3) in, can select for use potassium hydroxide, calcium hydroxide or sodium hydroxide reagent to regulate the soybean protein pH value of aqueous solution, but preferred potassium hydroxide reagent is regulated the soybean protein pH value of aqueous solution, this be because: potassium hydroxide aqueous solution is harmless, and can not cause solution to cross sticking and the influence filtration, potassium ion is the essential ion in the human body cell solution simultaneously, can be that body replenishes potassium ion.And adopt calcium hydroxide reagent to regulate the soybean protein pH value of aqueous solution, have dregs in the enzymolysis coarse filtration liquid behind its enzymolysis to occur; Adopt sodium hydroxide reagent to regulate the soybean protein pH value of aqueous solution, influence the Baume gravity (° B é) of filtrate, reduce filtering separation speed, both all influence the quality product of soybean polypeptide.
Further, above-mentioned steps 3) in, selecting enzyme activity unit for use is the Sumizyme MP of 600,000 U/g, the Sumizyme MP produced of Denmark Novozymes Company for example, its specificity height, stable performance, zymolysis technique maturation, only need to get final product by the indication operation of its catalogue, the enzymolysis intermediate product is easy to control.
Further, above-mentioned steps 5) in, can select for use oxalic acid, citric acid, oxysuccinic acid to come the pH value of regulator solution, and preferred oxalic acid reagent adjusting soybean polypeptide pH value of filtrate is because adopt oxalic acid reagent, its consumption is little, and the speed that takes effect is fast, if adopt citric acid reagent or oxysuccinic acid reagent to regulate the pH value, not only consumption is big, and tart flavour is heavier.
Advantage of the present invention: select for use microwave to urge zymolysis technique, reaction times is short, tens hours even more reaction times are shortened to tens of minutes, and to product structure and local flavor without any unpleasant odor, product color is good, thereby quality and the effect that finally can guarantee gained soybean polypeptide product are good.
Figure of description
Fig. 1 promotes that with microwave soybean protein hydrolysis prepares the soybean polypeptide schema for the present invention
Embodiment
The present invention is described in further detail below in conjunction with specific embodiment:
Embodiment 1: the first step, take by weighing soybean protein 1000g, and after its alkali extraction and acid precipitation clean is handled, obtain soybean protein isolate; Second step, soybean protein isolate is put into 5000ml microwave special purpose reactor, add in the distilled water or pure water of 2000ml, stir, regulate its pH value to 7.0 with potassium hydroxide, place microwave equipment, open microwave generator, power 500W, temperature rises under 100 ℃ of conditions and kept 10 minutes, temperature is reduced to 30 ℃, obtains the soybean protein isolate aqueous solution; The 3rd step, press 2% of soybean protein raw material weight again, get Sumizyme MP, join in the soybean protein aqueous solution of gained, and open microwave generator, power 500W, heat temperature raising to 40 ℃ carries out enzymolysis, and the short enzymolysis time of microwave was kept 30 minutes, obtained the soybean protein isolate enzymolysis solution; The 4th step with the hydrolysate of soybean protein liquid microwave heating to 90 of gained ℃, kept 2 minutes enzymic activitys of going out, and it was cooled to 50 ℃ then, carried out micro-filtrate membrane filtration, removed filter residue, acquisition hydrolysate of soybean protein filtrate; In the 5th step,, regulate its pH value to 4.0 with oxalic acid with adding the activity charcoal powder that accounts for its volume 1% in the hydrolysate of soybean protein filtrate of gained, keep that its temperature is decoloured under 50 ℃ of conditions, debitterize handled 1 hour, then it is filtered, remove the waste active carbon slag, obtain the smart filtrate of soybean polypeptide; At last, the smart filtrate of the soybean polypeptide of gained is carried out cryogenic vacuum concentrates, spray drying treatment obtains soyabean polypeptide powder.
Embodiment 2: the first step, take by weighing soybean protein 1500g, and after its alkali extraction and acid precipitation clean is handled, obtain soybean protein isolate; Second step, soybean protein isolate is put into 5000ml microwave special purpose reactor, add in the distilled water or pure water of 3000ml, stir, regulate its pH value to 8.0 with potassium hydroxide, place microwave equipment, open microwave generator, power 800W, temperature rises under 100 ℃ of conditions and kept 20 minutes, temperature is reduced to 30 ℃, obtains the soybean protein isolate aqueous solution; The 3rd step, press 3% of soybean protein raw material weight again, get Sumizyme MP, join in the soybean protein aqueous solution of gained, and open microwave generator, power 800W, heat temperature raising to 50 ℃ carries out enzymolysis, and the short enzymolysis time of microwave was kept 20 minutes, obtained the soybean protein isolate enzymolysis solution; The 4th step with the hydrolysate of soybean protein liquid microwave heating to 90 of gained ℃, kept 5 minutes enzymic activitys of going out, and it was cooled to 40 ℃ then, carried out micro-filtrate membrane filtration, removed filter residue, acquisition hydrolysate of soybean protein filtrate; In the 5th step,, regulate its pH value to 5.0 with oxalic acid with adding the activity charcoal powder that accounts for its volume 2% in the hydrolysate of soybean protein filtrate of gained, keep that its temperature is decoloured under 40 ℃ of conditions, debitterize handled 2 hours, then it is filtered, remove the waste active carbon slag, obtain the smart filtrate of soybean polypeptide; At last, the smart filtrate of the soybean polypeptide of gained is carried out cryogenic vacuum concentrates, spray drying treatment obtains soyabean polypeptide powder.
Embodiment 3: the first step, take by weighing soybean protein 2000g, and after its alkali extraction and acid precipitation clean is handled, obtain soybean protein isolate; Second step, soybean protein isolate is put into 5000ml microwave special purpose reactor, add in the distilled water or pure water of 4000ml, stir, regulate its pH value to 9.0 with potassium hydroxide, place microwave equipment, open microwave generator, power 1000W, temperature rises under 100 ℃ of conditions and kept 30 minutes, temperature is reduced to 30 ℃, obtains the soybean protein isolate aqueous solution; The 3rd step, press 6% of soybean protein raw material weight again, get Sumizyme MP, join in the soybean protein aqueous solution of gained, and open microwave generator, power 1000W, heat temperature raising to 60 ℃ carries out enzymolysis, and the short enzymolysis time of microwave was kept 10 minutes, obtained the soybean protein isolate enzymolysis solution; The 4th step with the hydrolysate of soybean protein liquid microwave heating to 90 of gained ℃, kept 8 minutes enzymic activitys of going out, and it was cooled to 30 ℃ then, carried out micro-filtrate membrane filtration, removed filter residue, acquisition hydrolysate of soybean protein filtrate; In the 5th step,, regulate its pH value to 6.0 with oxalic acid with adding the activity charcoal powder that accounts for its volume 3% in the hydrolysate of soybean protein filtrate of gained, keep that its temperature is decoloured under 30 ℃ of conditions, debitterize handled 1 hour, then it is filtered, remove the waste active carbon slag, obtain the smart filtrate of soybean polypeptide; At last, the smart filtrate of the soybean polypeptide of gained is carried out cryogenic vacuum concentrates, spray drying treatment obtains soyabean polypeptide powder.
Following table is to the prepared product index that goes out soyabean polypeptide powder of the foregoing description:
| Sequence number | Project | Requirement |
| 1 | Proterties | The white powdery, the visible impurity of no naked eyes does not lump, and nothing is gone mouldy. |
| 2 | Protein content, % | 〉=90.0 |
| 3 | Content of peptides, % | 〉=85.0 |
| 4 | Ash content, % | ≤ 5.0 |
| 5 | Moisture, % | ≤ 3.0 |
| 6 | Molecular weight (＜1000D) % | 〉=85.0 |
| 7 | Plumbous (in Pb), mg/kg | ≤ 0.5 |
| 8 | Arsenic (in As), mg/kg | ≤0.3 |
| 9 | Mercury, mg/kg | ≤0.3 |
| 10 | Total number of bacterial colony, cfu/g | ≤5000 |
| 11 | Coliform, MPN/100g | ≤20 |
| 12 | Yeast, cfu/g | ≤20 |
| 13 | Mould, cfu/g | ≤20 |
| 14 | Pathogenic bacterium | Must not detect |
Claims (1)
1. one kind promotes soybean protein hydrolysis to prepare the method for soybean polypeptide with microwave, in turn includes the following steps:
1) take by weighing soybean protein 100～1000g, carry out the alkali extraction and acid precipitation technology, cleaning is removed impurity and is obtained soybean protein isolate;
2) soybean protein isolate that obtains is put into microwave reactor, add in the distilled water or pure water of 2000～4000ml, stir, regulate its pH value to 7.0～9.0 with potassium hydroxide, place under the microwave condition to keep 3～15 minutes, microwave power 500～1000w carries out denaturing treatment to soybean protein isolate, cooling obtains the soybean protein isolate aqueous solution;
3) press 2～6% of soybean protein isolate raw material weight again, get Sumizyme MP, join step 2) in the soybean protein aqueous solution of gained, opening microwave generator makes temperature rise to 30～70 ℃ to carry out enzymolysis, microwave power 100-1000w, micro-wave energy continues supply during the enzymolysis, and the microwave enzymolysis time was kept 5～30 minutes, obtains the soybean protein isolate enzymolysis solution;
4) the soybean protein isolate enzymolysis solution microwave power with the step 3) gained is increased to 500～2000w, keeps 2～10 minutes enzymic activitys of going out more than 90 ℃, it is cooled to 30～50 ℃ then, carries out micro-filtrate membrane filtration, removes filter residue, obtains hydrolysate of soybean protein filtrate;
5) activity charcoal powder that accounts for its volume 1%～3% will be added in the hydrolysate of soybean protein filtrate of step 4) gained, regulate its pH value to 3.0～7.5 with oxalic acid, keep that its temperature is decoloured under 30～80 ℃ of conditions, debitterize handled 1～3 hour, then it is filtered, remove the waste active carbon slag, obtain the smart filtrate of soybean polypeptide;
6) the smart filtrate of the soybean polypeptide of step 5) gained is carried out cryogenic vacuum concentrates, spray drying treatment obtains soyabean polypeptide powder.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102334588A (en) * | 2011-08-26 | 2012-02-01 | 山东省花生研究所 | Preparation method for enzyme-modified peanut protein |
| CN103571900A (en) * | 2012-07-20 | 2014-02-12 | 诺利如一（安阳）生物科技有限公司 | Preparation and efficacy of low molecular weight soybean peptide |
| CN107201388A (en) * | 2017-06-05 | 2017-09-26 | 深圳知本康业有限公司 | A kind of milk protein polypeptide and application |
| CN109628536A (en) * | 2018-11-27 | 2019-04-16 | 安徽博悦生物科技有限公司 | A kind of preparation method of Soyprotein peptide |
| CN111920059A (en) * | 2020-06-28 | 2020-11-13 | 华南理工大学 | Soybean ACE inhibitory peptide and preparation method and application thereof |
| CN112575048A (en) * | 2020-12-15 | 2021-03-30 | 杭州益森键生物科技有限公司 | Development method of pine tree immunity inducing agent |
| CN113383852A (en) * | 2021-03-13 | 2021-09-14 | 青岛优合胜肽循环农业科技有限公司 | Preparation method for forming polypeptide by utilizing soybean hydrolysis |
| CN114468117A (en) * | 2022-03-01 | 2022-05-13 | 佳木斯冬梅大豆食品有限公司 | Preparation method of soybean protein isolate with high solubility |
| CN114836500A (en) * | 2022-01-26 | 2022-08-02 | 青岛孺子牛生物技术有限公司 | Production and preparation method for forming functional polypeptide by utilizing bean pulp enzymolysis |
| WO2022242386A1 (en) * | 2021-05-21 | 2022-11-24 | 江南大学 | Bifunctional bean-derived polypeptide and preparation method therefor |
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2009
- 2009-12-10 CN CN2009102731941A patent/CN102094059A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102334588A (en) * | 2011-08-26 | 2012-02-01 | 山东省花生研究所 | Preparation method for enzyme-modified peanut protein |
| CN102334588B (en) * | 2011-08-26 | 2013-04-03 | 山东省花生研究所 | Preparation method for enzyme-modified peanut protein |
| CN103571900A (en) * | 2012-07-20 | 2014-02-12 | 诺利如一（安阳）生物科技有限公司 | Preparation and efficacy of low molecular weight soybean peptide |
| CN107201388A (en) * | 2017-06-05 | 2017-09-26 | 深圳知本康业有限公司 | A kind of milk protein polypeptide and application |
| CN109628536A (en) * | 2018-11-27 | 2019-04-16 | 安徽博悦生物科技有限公司 | A kind of preparation method of Soyprotein peptide |
| CN111920059B (en) * | 2020-06-28 | 2022-06-14 | 华南理工大学 | Soybean ACE inhibitory peptide and preparation method and application thereof |
| CN111920059A (en) * | 2020-06-28 | 2020-11-13 | 华南理工大学 | Soybean ACE inhibitory peptide and preparation method and application thereof |
| CN112575048A (en) * | 2020-12-15 | 2021-03-30 | 杭州益森键生物科技有限公司 | Development method of pine tree immunity inducing agent |
| CN113383852A (en) * | 2021-03-13 | 2021-09-14 | 青岛优合胜肽循环农业科技有限公司 | Preparation method for forming polypeptide by utilizing soybean hydrolysis |
| WO2022242386A1 (en) * | 2021-05-21 | 2022-11-24 | 江南大学 | Bifunctional bean-derived polypeptide and preparation method therefor |
| CN114836500A (en) * | 2022-01-26 | 2022-08-02 | 青岛孺子牛生物技术有限公司 | Production and preparation method for forming functional polypeptide by utilizing bean pulp enzymolysis |
| CN114836500B (en) * | 2022-01-26 | 2024-04-30 | 青岛孺子牛生物技术有限公司 | Production and preparation method for forming functional polypeptide by utilizing enzymolysis of soybean meal |
| CN114468117A (en) * | 2022-03-01 | 2022-05-13 | 佳木斯冬梅大豆食品有限公司 | Preparation method of soybean protein isolate with high solubility |
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Application publication date: 20110615 |