CN102094057A - Method for preparing ovalbumin polypeptide by microwave-promoted enzymolysis - Google Patents
Method for preparing ovalbumin polypeptide by microwave-promoted enzymolysis Download PDFInfo
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- CN102094057A CN102094057A CN2009102731922A CN200910273192A CN102094057A CN 102094057 A CN102094057 A CN 102094057A CN 2009102731922 A CN2009102731922 A CN 2009102731922A CN 200910273192 A CN200910273192 A CN 200910273192A CN 102094057 A CN102094057 A CN 102094057A
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- ovalbumin
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Abstract
The invention discloses a method for preparing ovalbumin polypeptide by microwave-promoted hydrolysis of ovalbumin, which mainly fills in the blank of preparing the ovalbumin polypeptide from the ovalbumin in a short time in China. The ovalbumin polypeptide is prepared by a method of combining a microwave technology with a biological enzymolysis technology. By the method, a polypeptide product which has good appearance, good taste, strong functions, short preparation time and low production cost is developed. The method has the prominent advantages that: the ovalbumin polypeptide prepared by a microwave-promoted enzymolysis technology is superior to that prepared by the conventional enzymolysis technology; the obtained product does not contain impurities, has small molecular weight and is more easily absorbed and used by human bodies; and the process is simple, the reaction period is short, the enzymolysis degree is easy to control, and the product has high quality and good effects.
Description
Technical field
The present invention relates to quicken the processing technology of protein polypeptide hydrolysis, specifically refer to promote the ovalbumin hydrolysis to prepare the method for ovalbumin polypeptide with microwave.
Background technology
Albumen protein contains 8 seed amino acids of needed by human as the generally acknowledged high-quality protein of nutrition circle, and balanced proportion, body utilization ratio height.Simultaneously, because the nutritive ingredient of albumen and molecular structure and human plasma albumin are quite similar, thereby have the nutrition and health care effect, also be a kind of very ideal nutrition-fortifying agent.The ovalbumin polypeptide is easily absorbed by the body, and has the effect that strengthens body immunity.The preparation of ovalbumin polypeptide prepares in the enzymolysis mode usually, and relative cycle is longer, and the production cost height is subjected to certain limitation in Application in Food.And ovalbumin is prepared into the ovalbumin polypeptide in a short period of time with the short zymolysis technique of microwave, reaction times shortens to tens minutes, compared with conventional enzymolysis or fermentation technique need a few hours, the reaction time of tens hours even a couple of days, shortened the reaction times greatly, this technology market at home still belongs to blank.
Summary of the invention
Purpose of the present invention is exactly will provide a kind of to promote the ovalbumin hydrolysis to prepare the method for ovalbumin polypeptide with microwave.The heat effect and the cavitation effect of utilization microwave use biological enzymolysis technology that the ovalbumin polypeptide is prepared simultaneously.Adopt this method technology simple, the enzymolysis degree is easy to control, and gained ovalbumin polypeptide purity height, effect are good.What for achieving the above object, the present invention was designed promotes the egg white egg with microwave
Plain boiled water is separated the method for preparing the ovalbumin polypeptide, in turn includes the following steps:
1) takes by weighing fresh hen egg 1000-2000g, clean, boil 20min, remove yolk, eggshell obtains ovalbumin;
2) with the ovalbumin homogenate that obtains, put into 5000ml special microwave reactor, add in the distilled water or pure water of 2000-4000ml, stir;
3) regulate its pH value to 7.0-9.0 with potassium hydroxide, press the 0.5-2% of egg white protein isolate raw material weight again, get egg white hydrolysis special protein enzyme, join step 2) in the ovalbumin aqueous solution of gained, open microwave generator and make temperature rise to 50-70 ℃ to carry out enzymolysis, microwave power 100-500w, micro-wave energy continues supply during the enzymolysis, the microwave enzymolysis time was kept 10-20 minute, obtained the ovalbumin enzymolysis solution;
4) the egg white protein isolate enzymolysis solution microwave power with the step 3) gained is increased to 500-2000w,
100 ℃ keep the 2-10 minute enzymic activity of going out, and it are cooled to 30-50 ℃ then, carry out micro-filtrate membrane filtration, remove filter residue, obtain ovalbumin enzymolysis filtrate;
5) the ovalbumin polypeptide filtrate of step 4) gained is carried out cryogenic vacuum concentrates, spray drying treatment obtains the ovalbumin polypeptide powder.
Further, above-mentioned steps 3) in, can select for use potassium hydroxide, calcium hydroxide or sodium hydroxide reagent to regulate the ovalbumin pH value of aqueous solution, but preferred potassium hydroxide reagent is regulated the ovalbumin pH value of aqueous solution, this be because: potassium hydroxide aqueous solution is harmless, and can not cause solution to cross sticking and the influence filtration, potassium ion is the essential ion in the human body cell solution simultaneously, can be that body replenishes potassium ion.And adopt calcium hydroxide reagent to regulate the ovalbumin pH value of aqueous solution, have dregs in the enzymolysis coarse filtration liquid behind its enzymolysis to occur; Adopt sodium hydroxide reagent to regulate the ovalbumin pH value of aqueous solution, influence the Baume gravity of filtrate, reduce filtering separation speed, both all influence the quality product of egg white polypeptide.
Further, above-mentioned steps 3) in, selecting enzyme activity unit for use is the ovalbumin hydrolysis specific enzyme of 800,000 U/g, the outstanding proteolysis specific enzyme enzyme of can company of section producing of Spain for example, its specificity height, stable performance, zymolysis technique maturation, only need to get final product by the indication operation of its catalogue, the enzymolysis intermediate product is easy to control.
The present invention has obtained following technique effect:
1, select for use microwave to urge zymolysis technique, the reaction times is short, and tens hours even more reaction times are shortened to tens of minutes.
2, to product structure and local flavor without any unpleasant odor, product color is good, thus quality and the effect that finally can guarantee gained egg white polypeptide products are good.
Figure of description
Fig. 1 prepares ovalbumin polypeptide schema for the present invention with the short enzymolysis of microwave.
Embodiment
Understand and implement the present invention for the ease of those of ordinary skills, below embodiment the present invention is described in further detail.
The present invention is described in further detail below in conjunction with specific embodiment:
Embodiment 1: the first step, take by weighing ovalbumin 1000g, and after the pre-treatment (removing yolk, eggshell), obtain ovalbumin; Second step, after ovalbumin homogenate, put into 5000ml microwave special purpose reactor, add in the distilled water or pure water of 2000ml, stir, regulate its pH value to 7.0, place microwave equipment with potassium hydroxide, open microwave generator, power 100W, temperature rises to 50 ℃; The 3rd step, press 1% of ovalbumin raw material weight again, get ovalbumin hydrolysis specific enzyme, join in the ovalbumin aqueous solution of gained, and open microwave generator, power 100W, heat temperature raising to 50 ℃ carries out enzymolysis, and the short enzymolysis time of microwave was kept 30 minutes, obtained the ovalbumin enzymolysis solution; In the 4th step, unlatching microwave generator 1000w is heated to 100 ℃ with the ovalbumin enzymolysis solution of gained, keeps 5 minutes enzymic activitys of going out, and it is cooled to 30 ℃ then, carries out micro-filtrate membrane filtration, removes filter residue, obtains ovalbumin enzymolysis filtrate; At last, the ovalbumin enzymolysis filtrate of gained is carried out cryogenic vacuum concentrates, spray drying treatment obtains the ovalbumin polypeptide powder.
Embodiment 2: the first step, take by weighing ovalbumin 1500g, and after the pre-treatment (removing yolk, eggshell), obtain ovalbumin; Second step, after ovalbumin homogenate, put into 5000ml microwave special purpose reactor, add in the distilled water or pure water of 3000ml, stir, regulate its pH value to 7.5, place microwave equipment with potassium hydroxide, open microwave generator, power 300W, temperature rises to 60 ℃; The 3rd step, press 1.5% of ovalbumin raw material weight again, get ovalbumin hydrolysis specific enzyme, join in the ovalbumin aqueous solution of gained, and open microwave generator, power 300W, heat temperature raising to 60 ℃ carries out enzymolysis, and the short enzymolysis time of microwave was kept 20 minutes, obtained the ovalbumin enzymolysis solution; The 4th step was opened microwave generator 800w, with the ovalbumin enzymolysis solution microwave heating to 100 of gained ℃, kept 8 minutes enzymic activitys of going out, and it was cooled to 40 ℃ then, carried out micro-filtrate membrane filtration, removed filter residue, acquisition ovalbumin enzymolysis filtrate; At last, the ovalbumin enzymolysis filtrate of gained is carried out cryogenic vacuum concentrates, spray drying treatment obtains the ovalbumin polypeptide powder.
Embodiment 3: the first step, take by weighing ovalbumin 2000g, and after the pre-treatment (removing yolk, eggshell), obtain ovalbumin; Second step, after ovalbumin homogenate, put into 5000ml microwave special purpose reactor, add in the distilled water or pure water of 4000ml, stir, regulate its pH value to 8.0, place microwave equipment with potassium hydroxide, open microwave generator, power 500W, temperature rises to 70 ℃; The 3rd step, press 2.0% of ovalbumin raw material weight again, get ovalbumin hydrolysis specific enzyme, join in the ovalbumin aqueous solution of gained, and open microwave generator, power 500W, heat temperature raising to 70 ℃ carries out enzymolysis, and the short enzymolysis time of microwave was kept 15 minutes, obtained the ovalbumin enzymolysis solution; The 4th step, open microwave generator 500w with the ovalbumin enzymolysis solution microwave heating to 100 of gained ℃, keeps 10 minutes enzymic activitys of going out, it is cooled to 50 ℃ then, carry out micro-filtrate membrane filtration, remove filter residue, acquisition ovalbumin enzymolysis filtrate; At last, the ovalbumin enzymolysis filtrate of gained is carried out cryogenic vacuum concentrates, spray drying treatment obtains the ovalbumin polypeptide powder.
Following table is to the prepared product index that goes out the egg white polypeptide powder of the foregoing description:
| Sequence number | Project | Requirement |
| 1 | Proterties | The white powdery, the visible impurity of no naked eyes does not lump, and nothing is gone mouldy. |
| 2 | Protein content, % | 〉=75.0 |
| 3 | Content of peptides, % | 〉=70.0 |
| 4 | Ash content, % | ≤ 5.0 |
| 5 | Moisture, % | ≤ 3.0 |
| 6 | Molecular weight (＜1000D) % | 〉=90.0 |
| 7 | Plumbous (in Pb), mg/kg | ≤ 0.5 |
| 8 | Arsenic (in As), mg/kg | ≤ 0.3 |
| 9 | Mercury, mg/kg | ≤ 0.3 |
| 10 | Total number of bacterial colony, cfu/g | ≤ 5000 |
| 11 | Coliform, MPN/100g | ≤ 20 |
| 12 | Yeast, cfu/g | ≤ 20 |
| 13 | Mould, cfu/g | ≤ 20 |
| 14 | Pathogenic bacterium | Must not detect |
Claims (4)
1. one kind prepares the method for ovalbumin polypeptide with the short enzymolysis of microwave, it is characterized in that may further comprise the steps:
1) take by weighing fresh hen egg, cleaning is removed impurity and is obtained ovalbumin;
2) ovalbumin that obtains is put into the special microwave reactor, add distilled water or pure water, stir;
3) regulate its pH value to 7.0-9.0 with potassium hydroxide, press the certain proportion of egg white protein isolate raw material weight again, get ovalbumin hydrolysis specific enzyme, join step 2) in the ovalbumin aqueous solution of gained, opening microwave generator makes temperature rise to 50-70 ℃ to carry out enzymolysis, obtain the ovalbumin enzymolysis solution;
4) the ovalbumin enzymolysis solution microwave power with the step 3) gained is increased to 500-1000w, keeps the 2-10 minute enzymic activity of going out, and it is cooled to 30-50 ℃ then, carries out micro-filtrate membrane filtration, removes filter residue, obtains ovalbumin enzymolysis filtrate;
5) the ovalbumin enzymolysis filtrate of step 4) gained is carried out cryogenic vacuum concentrates, spray drying treatment obtains the ovalbumin polypeptide powder.
2. as claimed in claim 1ly prepare the method for ovalbumin polypeptide with the short enzymolysis of microwave, it is characterized in that: the fresh hen egg in the described step 1) is 1000-2000g.
3. as claimed in claim 1ly prepare the method for ovalbumin polypeptide with the short enzymolysis of microwave, it is characterized in that: the microwave reactor capacity described step 2) is 5000ml, and described distilled water or pure water are 2000-4000ml.
4. the method for preparing the ovalbumin polypeptide with the short enzymolysis of microwave as claimed in claim 1, it is characterized in that: the certain proportion in the described step 3) is 0.5-2%, microwave power when described microwave generator carries out enzymolysis is 100-500w, microwave continues supply during the enzymolysis, and the microwave enzymolysis time was kept 5-30 minute.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102334588A (en) * | 2011-08-26 | 2012-02-01 | 山东省花生研究所 | Preparation method for enzyme-modified peanut protein |
| EP3009011A2 (en) | 2014-10-16 | 2016-04-20 | Boguslaw Krzysztof Szostak | Homogenised product for particular nutritional uses |
| CN105876076A (en) * | 2015-01-17 | 2016-08-24 | 内蒙古天奇生物科技有限公司 | Method for preparing bone polypeptides through microwave-assisted enzymolysis |
| US20180008678A1 (en) * | 2016-07-06 | 2018-01-11 | Dongliang Chen | Preparation Method of the Albumin Peptide Combination and the Action of Inhibiting the Proliferation of Cancer Cells Thereof |
| CN113215215A (en) * | 2021-05-26 | 2021-08-06 | 杭州佰倍优生物科技有限公司 | Preparation method of albumin active peptide based on enzymolysis of egg white |
| CN114874315A (en) * | 2021-02-05 | 2022-08-09 | 承功（厦门）生物科技有限公司 | Preparation method of egg white polypeptide |
-
2009
- 2009-12-10 CN CN2009102731922A patent/CN102094057A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102334588A (en) * | 2011-08-26 | 2012-02-01 | 山东省花生研究所 | Preparation method for enzyme-modified peanut protein |
| CN102334588B (en) * | 2011-08-26 | 2013-04-03 | 山东省花生研究所 | Preparation method for enzyme-modified peanut protein |
| EP3009011A2 (en) | 2014-10-16 | 2016-04-20 | Boguslaw Krzysztof Szostak | Homogenised product for particular nutritional uses |
| CN105876076A (en) * | 2015-01-17 | 2016-08-24 | 内蒙古天奇生物科技有限公司 | Method for preparing bone polypeptides through microwave-assisted enzymolysis |
| US20180008678A1 (en) * | 2016-07-06 | 2018-01-11 | Dongliang Chen | Preparation Method of the Albumin Peptide Combination and the Action of Inhibiting the Proliferation of Cancer Cells Thereof |
| US10434148B2 (en) * | 2016-07-06 | 2019-10-08 | Dongliang Chen | Preparation method of albumin peptide combination having the action of inhibiting the proliferation of cancer cells |
| CN114874315A (en) * | 2021-02-05 | 2022-08-09 | 承功（厦门）生物科技有限公司 | Preparation method of egg white polypeptide |
| CN114874315B (en) * | 2021-02-05 | 2024-03-19 | 承功（厦门）生物科技有限公司 | Preparation method of egg white polypeptide |
| CN113215215A (en) * | 2021-05-26 | 2021-08-06 | 杭州佰倍优生物科技有限公司 | Preparation method of albumin active peptide based on enzymolysis of egg white |
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Application publication date: 20110615 |