CN101724676A - Method for preparing almond polypeptide from raw material of almond protein - Google Patents
Method for preparing almond polypeptide from raw material of almond protein Download PDFInfo
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- CN101724676A CN101724676A CN200810197418A CN200810197418A CN101724676A CN 101724676 A CN101724676 A CN 101724676A CN 200810197418 A CN200810197418 A CN 200810197418A CN 200810197418 A CN200810197418 A CN 200810197418A CN 101724676 A CN101724676 A CN 101724676A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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Abstract
The invention discloses a method for preparing almond polypeptide from the raw material of almond protein, particularly comprising the steps of degreasing the almond protein with a carbon dioxide supercritical extracting device and biologically decomposing the almond protein with protease. The method for preparing almond polypeptide from the raw material of almond protein is simple and is easy to control the degree of decomposing the almond protein with the protease. The prepared almond polypeptide has high purity, good efficacy and low molecular weight and can be easily absorbed and utilized by the human body.
Description
Technical field
The present invention relates to the deep process technology of almond, specifically refer to be the method for feedstock production almond polypeptide with the almond protein.
Background technology
Apricot is that China originates in one of fruit tree, and people are searched for food before the history of apricot fruit can trace back to 5000-6000, and the cultivation history that has good grounds also has 3500.In recent years, be worth because people recognize the multiple nutrients of almond, benevolence is enlarged rapidly with the cultivated area of apricot at China's wide geographic area.Almond is the dry seed of apricot fruit, and two kinds of Semen Armeniacae Amarum and Sweet Apricot seeds are arranged.In general, Semen Armeniacae Amarum is mainly made medicinal material and is used, Sweet Apricot seed uses as food mostly, contain abundant grease in the almond, its oleaginousness is about 50%, be soybean oleaginousness (20%-22%) 2.5-3.1 doubly, suitable with the oleaginousness of peanut (39.2%-41.3%), sunflower seeds (54.5%), sesame (47.3%-48.0%), and mostly be unsaturated fatty acids in the Prunus amygdalus oil greatly.In addition, contain rich in protein in the almond, contain 18 seed amino acids of human body needs, can be used as a kind of proteinaceous nutrient reinforcer and additive, alleviate the shortage of protein resource.Yet along with the development of Prunus amygdalus oil processing industry, the almond protein that accounts in the industrial wood waste more than 60% does not make full use of, and these protein often go out of use or as just feed.
Summary of the invention
Purpose of the present invention is exactly that will to provide a kind of be the method for feedstock production almond polypeptide with the almond protein, improves the utilization ratio of almond protein greatly, effectively alleviates the shortage of protein resource.
It is the method for feedstock production almond polypeptide with the almond protein that technical scheme of the present invention provides a kind of, in turn includes the following steps:
1) takes by weighing the some grams of almond protein raw material, put it in the extraction plant skimming treatment 4～8 hours, obtain the almond protein after the skimming treatment;
2) almond protein that obtains in the above-mentioned steps 1 is put into container, add the distilled water or the pure water that enough soak into, stir, regulate its pH value to 7.0～9.0 with a pH value conditioning agent, place 30～50 ℃ temperature condition to keep 20～40 minutes down, almond protein is wherein fully dissolved, obtain the almond protein aqueous solution;
3) press 3～5% of raw material weight, get Sumizyme MP, add in the almond protein aqueous solution that obtains in the above-mentioned steps 2, and heat temperature raising to 50～70 ℃ carry out enzymolysis, continuously stirring during the enzymolysis, enzymolysis time was kept 4～6 hours, obtained the almond protein enzymolysis solution;
4) the almond protein enzymolysis solution with above-mentioned steps 3 gained is heated to 80～100 ℃, keeps 5～10 minutes enzymic activitys of going out, and it is cooled to 30～50 ℃ then, carries out coarse filtration, removes filter residue, obtains almond protein enzymolysis coarse filtration liquid;
5) activity charcoal powder that accounts for its volume 1%～3% will be added in the almond protein enzymolysis coarse filtration liquid of above-mentioned steps 4 gained, regulate its pH value to 3.0～5.0 with the 2nd pH value conditioning agent, keep that its temperature is decoloured under 30～50 ℃ of conditions, debitterize handled 1～3 hour, then it is filtered, remove the waste active carbon slag, obtain the smart filtrate of almond polypeptide;
6) the smart filtrate of the almond polypeptide of above-mentioned steps 5 gained is carried out vacuum concentration, spray drying treatment obtains the almond polypeptide powder.
Another technical scheme of the present invention is: a kind of is the method for feedstock production almond polypeptide with the almond protein, in turn includes the following steps:
1) takes by weighing almond protein 1000～2000g, put it in the carbon dioxide upercritical fluid extraction device skimming treatment 4～8 hours, obtain the almond protein after the skimming treatment;
2) almond protein that above-mentioned steps 1 is obtained is put into container, add in the distilled water or pure water of 2000～4000ml, stir, regulate its pH value to 7.0～9.0 with potassium hydroxide, place 30～50 ℃ temperature condition to keep 20～40 minutes down, do not stop to stir the almond protein that makes wherein and fully dissolve, obtain the almond protein aqueous solution;
3) press 3～5% of raw material weight, get Sumizyme MP, add in the almond protein aqueous solution that obtains in the above-mentioned steps 2, and heat temperature raising to 50～70 ℃ carry out enzymolysis, continuously stirring during the enzymolysis, enzymolysis time was kept 4～6 hours, obtained the almond protein enzymolysis solution;
4) the almond protein enzymolysis solution with above-mentioned steps 3 gained is heated to 80～100 ℃, keeps 5～10 minutes enzymic activitys of going out, and it is cooled to 30～50 ℃ then, carries out coarse filtration, removes filter residue, obtains almond protein enzymolysis coarse filtration liquid;
5) activity charcoal powder that accounts for its volume 1%～3% will be added in the almond protein enzymolysis coarse filtration liquid of above-mentioned steps 4 gained, regulate its pH value to 3.0～5.0 with oxalic acid, keep that its temperature is decoloured under 30～50 ℃ of conditions, debitterize handled 1～3 hour, then it is filtered, remove the waste active carbon slag, obtain the smart filtrate of almond polypeptide;
6) the smart filtrate of the almond polypeptide of above-mentioned steps 5 gained is carried out vacuum concentration, spray drying treatment obtains the almond polypeptide powder.
Of the present invention is that the method for feedstock production almond polypeptide selects for use the carbon dioxide upercritical fluid extraction device that almond protein is carried out the skimming treatment technology with the almond protein, not only impurity is few through the almond protein after the skimming treatment, and without any unpleasant odor, product color is good, thereby quality and the effect that finally can guarantee gained almond polypeptide product are good.Of the present invention is that the method technology of feedstock production almond polypeptide is simple with the almond protein, and the enzymolysis degree is easy to control, and products obtained therefrom purity height, effect is good, molecular weight is little, is absorbed by human body and utilizes easilier.
Embodiment
The present invention is described in further detail below in conjunction with specific embodiment:
Embodiment 1: the first step, take by weighing almond protein 1000g, and put it in the carbon dioxide upercritical fluid extraction device skimming treatment 4 hours, obtain the almond protein after the skimming treatment; Second step was put into the 5000ml beaker with almond protein, add in the distilled water or pure water of 2000ml, stir, regulate its pH value to 7.0 with potassium hydroxide, place 30 ℃ temperature condition to keep 20 minutes down, do not stop to stir the almond protein that makes wherein and fully dissolve, obtain the almond protein aqueous solution; The 3rd step, press 3% of almond protein raw material weight again, get Sumizyme MP, join in the almond protein aqueous solution of gained, and heat temperature raising to 50 ℃ carries out enzymolysis, continuously stirring during the enzymolysis, enzymolysis time was kept 4 hours, acquisition almond protein enzymolysis solution; The almond protein enzymolysis solution of the 4th step with gained is heated to 80 ℃, keeps 5 minutes enzymic activitys of going out, and it is cooled to 30 ℃ then, carries out coarse filtration, removes filter residue, obtains almond protein enzymolysis coarse filtration liquid; The 5th step, the activity charcoal powder that accounts for its volume 1% will be added in the almond protein enzymolysis coarse filtration liquid of gained, regulate its pH value to 3.0 with oxalic acid, keep that its temperature is decoloured under 30 ℃ of conditions, debitterize handled 1 hour, then it is filtered, remove the waste active carbon slag, obtain the smart filtrate of almond polypeptide; At last, the smart filtrate of the almond polypeptide of gained is carried out vacuum concentration, spray drying treatment obtains the almond polypeptide powder.
Embodiment 2: the first step, take by weighing almond protein 1500g, and put it in the carbon dioxide upercritical fluid extraction device skimming treatment 5 hours, obtain the almond protein after the skimming treatment; Second step was put into the 5000ml beaker with almond protein, add in the distilled water or pure water of 3000ml, stir, regulate its pH value to 8.0 with potassium hydroxide, place 40 ℃ temperature condition to keep 30 minutes down, do not stop to stir the almond protein that makes wherein and fully dissolve, obtain the almond protein aqueous solution; The 3rd step, press 4% of almond protein raw material weight again, get Sumizyme MP, join in the almond protein aqueous solution of gained, and heat temperature raising to 60 ℃ carries out enzymolysis, continuously stirring during the enzymolysis, enzymolysis time was kept 5 hours, acquisition almond protein enzymolysis solution; The almond protein enzymolysis solution of the 4th step with gained is heated to 90 ℃, keeps 8 minutes enzymic activitys of going out, and it is cooled to 40 ℃ then, carries out coarse filtration, removes filter residue, obtains almond protein enzymolysis coarse filtration liquid; The 5th step, the activity charcoal powder that accounts for its volume 2% will be added in the almond protein enzymolysis coarse filtration liquid of gained, regulate its pH value to 4.0 with oxalic acid, keep that its temperature is decoloured under 40 ℃ of conditions, debitterize handled 2 hours, then it is filtered, remove the waste active carbon slag, obtain the smart filtrate of almond polypeptide; At last, the smart filtrate of the almond polypeptide of gained is carried out vacuum concentration, spray drying treatment obtains the almond polypeptide powder.
Embodiment 3: the first step, take by weighing almond protein 2000g, and put it in the carbon dioxide upercritical fluid extraction device skimming treatment 6 hours, obtain the almond protein after the skimming treatment; Second step was put into the 5000ml beaker with almond protein, add in the distilled water or pure water of 4000ml, stir, regulate its pH value to 9.0 with potassium hydroxide, place 50 ℃ temperature condition to keep 40 minutes down, do not stop to stir the almond protein that makes wherein and fully dissolve, obtain the almond protein aqueous solution; The 3rd step, press 5% of almond protein raw material weight again, get Sumizyme MP, join in the almond protein aqueous solution of gained, and heat temperature raising to 70 ℃ carries out enzymolysis, continuously stirring during the enzymolysis, enzymolysis time was kept 6 hours, acquisition almond protein enzymolysis solution; The almond protein enzymolysis solution of the 4th step with gained is heated to 100 ℃, keeps 10 minutes enzymic activitys of going out, and it is cooled to 50 ℃ then, carries out coarse filtration, removes filter residue, obtains almond protein enzymolysis coarse filtration liquid; The 5th step, the activity charcoal powder that accounts for its volume 3% will be added in the almond protein enzymolysis coarse filtration liquid of gained, regulate its pH value to 5.0 with oxalic acid, keep that its temperature is decoloured under 50 ℃ of conditions, debitterize handled 3 hours, then it is filtered, remove the waste active carbon slag, obtain the smart filtrate of almond polypeptide; At last, the smart filtrate of the almond polypeptide of gained is carried out vacuum concentration, spray drying treatment obtains the almond polypeptide powder.
Regulate the almond protein pH value of aqueous solution in the embodiment of the invention and can select potassium hydroxide, calcium hydroxide or sodium hydroxide reagent for use, but preferred potassium hydroxide reagent is regulated the almond protein pH value of aqueous solution, this is because potassium hydroxide aqueous solution is harmless, and can not cause solution to cross sticking and the influence filtration, potassium ion is the essential ion in the human body cell solution simultaneously, can replenish potassium ion for body.And adopt calcium hydroxide reagent to regulate the almond protein pH value of aqueous solution, have dregs in the enzymolysis coarse filtration liquid behind its enzymolysis to occur; Adopt sodium hydroxide reagent to regulate the almond protein pH value of aqueous solution, the taste of its product is a bitter taste, and both all influence the quality product of almond polypeptide.
It is the Sumizyme MP of 200,000 U/g that Sumizyme MP in the embodiment of the invention is selected enzyme activity unit for use, the Alcalase Sumizyme MP produced of Denmark Novozymes Company for example, its specificity height, stable performance, the zymolysis technique maturation, simple to operate, the enzymolysis intermediate product is easy to control.
The thick pH value of filtrate of almond protein enzymolysis can be selected oxalic acid, citric acid, oxysuccinic acid for use in the embodiment of the invention, and the thick pH value of filtrate of preferred oxalic acid reagent adjusting almond protein enzymolysis is because adopt oxalic acid reagent, its consumption is little, the speed that takes effect is fast, regulate the pH value if adopt citric acid reagent or oxysuccinic acid reagent, not only consumption is big, and tart flavour is heavier.
Following table is to the prepared product index that goes out the almond polypeptide powder of the various embodiments described above:
| Sequence number | Project | Requirement |
| 1 | Proterties | Light yellow powdery, the visible impurity of no naked eyes does not lump, and nothing is gone mouldy. |
| 2 | Protein content, % | 〉=80.0 |
| Sequence number | Project | Requirement |
| ??3 | Ash content, % | ??≤4.58 |
| ??4 | Moisture, % | ??≤3.78 |
| ??5 | Molecular weight (＜1000D) % | ??≥80.0 |
| ??6 | Plumbous (in Pb), mg/kg | ??≤0.5 |
| ??7 | Arsenic (in As), mg/kg | ??≤0.3 |
| ??8 | Mercury, mg/kg | ??≤0.3 |
| ??9 | Total number of bacterial colony, cfu/g | ??≤20000 |
| ??10 | Coliform, MPN/100g | ??≤80 |
| ??11 | Yeast, cfu/g | ??≤20 |
| ??12 | Mould, cfu/g | ??≤20 |
| ??13 | Pathogenic bacterium | Must not detect |
Claims (8)
1. one kind is the method for feedstock production almond polypeptide with the almond protein, in turn includes the following steps:
1) takes by weighing the some grams of almond protein raw material, put it in the extraction plant skimming treatment 4～8 hours, obtain the almond protein after the skimming treatment;
2) almond protein that obtains in the above-mentioned steps 1 is put into container, add the distilled water or the pure water that enough soak into, stir, regulate its pH value to 7.0～9.0 with a pH value conditioning agent, place 30～50 ℃ temperature condition to keep 20～40 minutes down, almond protein is wherein fully dissolved, obtain the almond protein aqueous solution;
3) press 3～5% of raw material weight, get Sumizyme MP, add in the almond protein aqueous solution that obtains in the above-mentioned steps 2, and heat temperature raising to 50～70 ℃ carry out enzymolysis, continuously stirring during the enzymolysis, enzymolysis time was kept 4～6 hours, obtained the almond protein enzymolysis solution;
4) the almond protein enzymolysis solution with above-mentioned steps 3 gained is heated to 80～100 ℃, keeps 5～10 minutes enzymic activitys of going out, and it is cooled to 30～50 ℃ then, carries out coarse filtration, removes filter residue, obtains almond protein enzymolysis coarse filtration liquid;
5) activity charcoal powder that accounts for its volume 1%～3% will be added in the almond protein enzymolysis coarse filtration liquid of above-mentioned steps 4 gained, regulate its pH value to 3.0～5.0 with the 2nd pH value conditioning agent, keep that its temperature is decoloured under 30～50 ℃ of conditions, debitterize handled 1～3 hour, then it is filtered, remove the waste active carbon slag, obtain the smart filtrate of almond polypeptide;
6) the smart filtrate of the almond polypeptide of above-mentioned steps 5 gained is carried out vacuum concentration, spray drying treatment obtains the almond polypeptide powder.
2. according to claim 1 is the method for feedstock production almond polypeptide with the almond protein, it is characterized in that: utilization carbon dioxide upercritical fluid extraction device carries out skimming treatment to almond protein in the described step 1.
3. according to claim 1 is the method for feedstock production almond polypeptide with the almond protein, it is characterized in that: the amount of distilled water or pure water is preferably 1～2 times of almond protein raw material weight in the described step 2.
4. according to claim 1 is the method for feedstock production almond polypeptide with the almond protein, it is characterized in that: the pH value conditioning agent in the described step 2 can be potassium hydroxide, calcium hydroxide or sodium hydroxide reagent.
5. according to claim 1 is the method for feedstock production almond polypeptide with the almond protein, it is characterized in that: the Sumizyme MP enzyme activity unit in the described step 3 is 200,000 U/g.
6. according to claim 1 is the method for feedstock production almond polypeptide with the almond protein, it is characterized in that: the 2nd pH value conditioning agent in the described step 5 can be oxalic acid, citric acid, oxysuccinic acid.
7. one kind is the method for feedstock production almond polypeptide with the almond protein, in turn includes the following steps:
1) takes by weighing almond protein 1000～2000g, put it in the carbon dioxide upercritical fluid extraction device skimming treatment 4～8 hours, obtain the almond protein after the skimming treatment;
2) almond protein that above-mentioned steps 1 is obtained is put into container, add in the distilled water or pure water of 2000～4000ml, stir, regulate its pH value to 7.0～9.0 with potassium hydroxide, place 30～50 ℃ temperature condition to keep 20～40 minutes down, do not stop to stir the almond protein that makes wherein and fully dissolve, obtain the almond protein aqueous solution;
3) press 3～5% of raw material weight, get Sumizyme MP, add in the almond protein aqueous solution that obtains in the above-mentioned steps 2, and heat temperature raising to 50～70 ℃ carry out enzymolysis, continuously stirring during the enzymolysis, enzymolysis time was kept 4～6 hours, obtained the almond protein enzymolysis solution;
4) the almond protein enzymolysis solution with above-mentioned steps 3 gained is heated to 80～100 ℃, keeps 5～10 minutes enzymic activitys of going out, and it is cooled to 30～50 ℃ then, carries out coarse filtration, removes filter residue, obtains almond protein enzymolysis coarse filtration liquid;
5) activity charcoal powder that accounts for its volume 1%～3% will be added in the almond protein enzymolysis coarse filtration liquid of above-mentioned steps 4 gained, regulate its pH value to 3.0～5.0 with oxalic acid, keep that its temperature is decoloured under 30～50 ℃ of conditions, debitterize handled 1～3 hour, then it is filtered, remove the waste active carbon slag, obtain the smart filtrate of almond polypeptide;
6) the smart filtrate of the almond polypeptide of above-mentioned steps 5 gained is carried out vacuum concentration, spray drying treatment obtains the almond polypeptide powder.
8. according to claim 7 is the method for feedstock production almond polypeptide with the almond protein, it is characterized in that: the Sumizyme MP enzyme activity unit in the described step 3 is 200,000 U/g.
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| Application Number | Priority Date | Filing Date | Title |
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| CN200810197418A CN101724676A (en) | 2008-10-28 | 2008-10-28 | Method for preparing almond polypeptide from raw material of almond protein |
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| CN200810197418A CN101724676A (en) | 2008-10-28 | 2008-10-28 | Method for preparing almond polypeptide from raw material of almond protein |
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| CN101724676A true CN101724676A (en) | 2010-06-09 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102687891A (en) * | 2012-06-05 | 2012-09-26 | 北京林业大学 | Wild almond polypeptide beverage product and method for preparing same |
| CN103421875A (en) * | 2013-08-12 | 2013-12-04 | 石河子大学 | Method for preparing almond protein antioxidant peptides of little white apricot through enzymatic hydrolysis |
| CN103478783A (en) * | 2013-09-06 | 2014-01-01 | 李嘉 | Preparation method of sweet almond buccal tablets |
| CN105779536A (en) * | 2016-01-13 | 2016-07-20 | 张新宇 | Preparation process of almond blood pressure reducing functional oligopeptides |
| CN112205626A (en) * | 2020-08-28 | 2021-01-12 | 荣鼎(广东)生物科技有限公司 | Production process of protein powder convenient to store |
-
2008
- 2008-10-28 CN CN200810197418A patent/CN101724676A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102687891A (en) * | 2012-06-05 | 2012-09-26 | 北京林业大学 | Wild almond polypeptide beverage product and method for preparing same |
| CN102687891B (en) * | 2012-06-05 | 2013-12-11 | 北京林业大学 | Wild almond polypeptide beverage product and method for preparing same |
| CN103421875A (en) * | 2013-08-12 | 2013-12-04 | 石河子大学 | Method for preparing almond protein antioxidant peptides of little white apricot through enzymatic hydrolysis |
| CN103421875B (en) * | 2013-08-12 | 2015-04-22 | 石河子大学 | Method for preparing almond protein antioxidant peptides of little white apricot through enzymatic hydrolysis |
| CN103478783A (en) * | 2013-09-06 | 2014-01-01 | 李嘉 | Preparation method of sweet almond buccal tablets |
| CN105779536A (en) * | 2016-01-13 | 2016-07-20 | 张新宇 | Preparation process of almond blood pressure reducing functional oligopeptides |
| CN112205626A (en) * | 2020-08-28 | 2021-01-12 | 荣鼎(广东)生物科技有限公司 | Production process of protein powder convenient to store |
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Application publication date: 20100609 |