CN101912029A - Method for preparing grain amaranth high protein powder by double enzyme process - Google Patents
Method for preparing grain amaranth high protein powder by double enzyme process Download PDFInfo
- Publication number
- CN101912029A CN101912029A CN 201010250995 CN201010250995A CN101912029A CN 101912029 A CN101912029 A CN 101912029A CN 201010250995 CN201010250995 CN 201010250995 CN 201010250995 A CN201010250995 A CN 201010250995A CN 101912029 A CN101912029 A CN 101912029A
- Authority
- CN
- China
- Prior art keywords
- powder
- value
- high protein
- reaction
- amaranthus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a method for preparing grain amaranth high protein powder by a double enzyme process, which aims to provide a high-protein-content, simple-process and low-cost method for preparing the grain amaranth high protein powder. The method comprises the following steps of: mixing the grain amaranth seed powder and water according to a weight ratio of 1: 40 to prepare material liquid, heating the material liquid in thermostatic water-bath to 45 to 55 DEG C, adjusting the pH value of the material liquid to 4.5 to 5.0 and then adding cellulose into the material liquid for reaction for 2 to 4h; after the enzymolysis, adjusting the pH value of the material liquid to 6.0 to 7.0 and then adding alpha-amylase for reaction at the temperature of between 50 and 60 DEG C for 1 to 3h; inactivating enzymes of the reaction liquid obtained after the enzymolysis for two times in a water bath at the temperature of 80 DEG C for 30min; adjusting the pH value of the reaction liquid after the enzyme inactivation to an isoelectric point of 4.6 to precipitate soluble protein; centrifuging the obtained liquid at the speed of 3,000r/min for 20min, discarding supernate and collecting precipitate; and freezing and drying the centrifuged precipitate at the temperature of 55 DEG C below zero for 48h to obtain the grain amaranth high protein powder. No harmful additives are used in the preparation process of the method of the invention and obtained products are safe to eat.
Description
Technical field
The present invention relates to a kind of method of preparing grain amaranth high protein powder by double enzyme process.
Background technology
At present, China mainly is aspect cultivation, trophic analysis and feed development utilize to the research of Grain Production of Amaranthus.By discovering, protein content in the Grain Production of Amaranthus seed and quality all are higher than general cereal, and the amino acid proportion balance, and its protein quality has surpassed soybean, approaches milk, and are rich in lysine and essential fatty acid, are well suited for the human nutrition needs.Grain Production of Amaranthus protein added to go not only to make in the food protein quantity to increase in other cereal foods, and quality optimization, nutritive value of food improves greatly.Therefore, Grain Production of Amaranthus is developed as a kind of new plant protein resource have broad prospects.
The report that the extraction of Grain Production of Amaranthus leaf protein is arranged at present, method mainly are that it mainly is the extraction of soluble protein with water extraction, acid hydrolyzation, lost water-insoluble albumen, so the recovery rate of protein is lower, and mainly is the application that applies to the feed aspect, is used for the less of food aspect.
Summary of the invention
The present invention is in order to overcome weak point of the prior art, provide a kind of be suitable for human edible, the protein content height, technology is simple, the method for producing the Grain Production of Amaranthus high protein powder that cost is low.
The present invention is achieved through the following technical solutions:
A kind of method of preparing grain amaranth high protein powder by double enzyme process is characterized in that, comprises the steps:
(1) enzymolysis: with Grain Production of Amaranthus powder of seeds and water is 1: 4 preparation feed liquid by weight, after water bath with thermostatic control is warming up to 45～55 ℃, adjusts material liquid pH value to 4.5～5.0 backs and adds cellulose, reaction 2～4h; The pH value of feed liquid behind the cellulase degradation is adjusted to 6.0～7.0 back adding AMSs, at 50～60 ℃ of reaction 1～3h; Again with the reactant liquor behind twice enzymolysis at 80 ℃ of water-baths enzyme 30min that goes out; Wherein, the enzyme concentration of cellulase is the 20.8U/g substrate, the enzyme concentration 6.6U/g substrate of AMS;
(2) precipitation, centrifugal, dry: the reactant liquor adjust pH behind the enzyme that will go out to isoelectric point 4.6 makes the soluble protein precipitation; The centrifugal 20min of 3000r/min abandons supernatant afterwards, gets precipitation; With being deposited in after centrifugal-55 ℃ freeze drying 48h, promptly make the Grain Production of Amaranthus high protein powder.
Wherein, the optimum reaction condition of step (1) is: with Grain Production of Amaranthus powder of seeds and water is 1: 40 preparation feed liquid by weight, after water bath with thermostatic control is warming up to 50 ℃, adjusts material liquid pH value to 4.8 back and adds the cellulase powder, reaction 3h; The pH value of the feed liquid behind the cellulase degradation is adjusted to 6.5 back adding AMS powder, at 55 ℃ of reaction 2h.Directly add the Grain Production of Amaranthus high protein powder nonhazardous effect of cellulase powder and AMS powder to making.
In the step (1), working concentration is the HCl solution of 1mol/L when adjusting material liquid pH value, working concentration is the NaOH solution of 1mol/L when regulating the pH value of feed liquid behind the cellulase degradation, and working concentration was the HCl solution of 1mol/L when enzyme afterreaction liquid pH value was gone out in adjusting in the step (2).
The present invention has following technique effect:
1. the present invention extracts albumen in the Grain Production of Amaranthus seed by two enzymes method, sloughs cellulose and starch in the Grain Production of Amaranthus seed by enzymolysis, like this can more effective comprehensive utilization Grain Production of Amaranthus seed albumen.Existing water-solubility protein has water-insoluble albumen again in the protein that the present invention extracts, and is most albumen in the Grain Production of Amaranthus seed, has kept original nutritional labeling.The Grain Production of Amaranthus high protein powder that obtains by method of the present invention is suitable for human eating, and the protein content height has improved 77.1% at most, and, manufacturing process does not have to add harmful material, can be used as the raw material of making human high protein healthy food, and product is safe to eat.
2. in the method for the present invention, use cellulase to make the composition that most of unedible cellulose hydrolysis becomes little molecule to be easy to digest and assimilate in the Grain Production of Amaranthus powder of seeds, improve the edibility of food, the protein of cellulose parcel is discharged, more help the extraction of protein.
3. in the method for the present invention, use AMS to make the starch hydrolysis in the Grain Production of Amaranthus powder of seeds be transformed into water-soluble sugar, and remove, improved Protein content in the product by centrifugal method.
4. the protein content in the Grain Production of Amaranthus albumen powder that obtains by method of the present invention is up to 28.56%, form through high effective liquid chromatography for measuring amino acid, wherein lysine accounts for 3.6%, and this albumen powder can be used as the batching of the lysine content that is used for improving food.
5, method of the present invention is simple, and cost is low, for Grain Production of Amaranthus provides new approach as a kind of new plant protein resource.
The specific embodiment
The present invention is described in detail below in conjunction with specific embodiment.
Embodiment 1
(1) getting 1000g Grain Production of Amaranthus powder of seeds and water is 1: 40 preparation feed liquid by weight, after water bath with thermostatic control is warming up to 45 ℃, is HCl solution adjustment material liquid pH value to the 4.5 back adding cellulase 20800U of 1mol/L with concentration, reaction 2h.With the pH value concentration of the feed liquid behind the cellulase degradation is that the NaOH solution of 1mol/L is adjusted to 6.0 backs and adds AMS 6600U, at 50 ℃ of reaction 1h.With the reactant liquor behind twice enzymolysis at 80 ℃ of water-baths enzyme 30min that goes out.
(2) will go out reactant liquor concentration behind the enzyme is that HCl solution adjust pH to the isoelectric point 4.6 of 1mol/L makes the soluble protein precipitation, and the centrifugal 20min of 3000r/min abandons supernatant afterwards, gets precipitation.With being deposited in after centrifugal-55 ℃ freeze drying 48h, make Grain Production of Amaranthus high protein powder 725g, record with Kjeldahl and contain protein 156g in the high protein powder.
Measure as can be known by Kjeldahl, contain protein 16.5% in the former Grain Production of Amaranthus powder of seeds of every 1000g, be equivalent to 165g.Former Grain Production of Amaranthus powder of seeds obtains high protein powder 725g by said method, wherein contain protein 156g, protein content is 21.52%, protein content by unit mass in more former Grain Production of Amaranthus powder of seeds and the resulting high protein powder of said method learns, with example 1 the high protein powder that obtains of method improved 30.42% than former Grain Production of Amaranthus powder of seeds protein content.
Embodiment 2
(1) getting 1000g Grain Production of Amaranthus powder of seeds and water is 1: 40 preparation feed liquid by weight, after water bath with thermostatic control is warming up to 55 ℃, is HCl solution adjustment material liquid pH value to the 5.0 back adding cellulase 20800U of 1mol/L with concentration, reaction 4h.With the pH value concentration of feed liquid behind the cellulase degradation is that the NaOH solution of 1mol/L is adjusted to 7.0 backs and adds AMS 6600U, at 60 ℃ of reaction 3h.With the reactant liquor behind twice enzymolysis at 80 ℃ of water-baths enzyme 30min that goes out.
(2) will go out reactant liquor concentration behind the enzyme is that HCl solution adjust pH to the isoelectric point 4.6 of 1mol/L makes the soluble protein precipitation, and the centrifugal 20min of 3000r/min abandons supernatant afterwards, gets precipitation.With being deposited in after centrifugal-55 ℃ freeze drying 48h, make Grain Production of Amaranthus high protein powder 595g, record with Kjeldahl and contain protein 155g in the high protein powder.
Measure as can be known by Kjeldahl, contain protein 16.5%, 165g in the former Grain Production of Amaranthus powder of seeds of every 1000g.Former Grain Production of Amaranthus powder of seeds obtains high protein powder 595g by said method, wherein contains protein 155g, and protein content is 26.05%, and the more former Grain Production of Amaranthus powder of seeds of protein content improves 57.88% in the high protein powder of embodiment 2 gained.
Embodiment 3
(1) getting 1000g Grain Production of Amaranthus powder of seeds and water is 1: 40 preparation feed liquid by weight, after water bath with thermostatic control is warming up to 50 ℃, is HCl solution adjustment material liquid pH value to the 4.8 back adding cellulase 20800U of 1mol/L with concentration, reaction 3h.With the pH value concentration of feed liquid behind the cellulase degradation is that the NaOH solution of 1mol/L is adjusted to 6.5 backs and adds AMS 6600U, at 55 ℃ of reaction 2h.With the reactant liquor behind twice enzymolysis at 80 ℃ of water-baths enzyme 30min that goes out.
(2) will go out reactant liquor concentration behind the enzyme is that HCl solution adjust pH to the isoelectric point 4.6 of 1mol/L makes the soluble protein precipitation, and the centrifugal 20min of 3000r/min abandons supernatant and gets precipitation afterwards.With being deposited in after centrifugal-55 ℃ freeze drying 48h, make Grain Production of Amaranthus high protein powder 541.5g, record with Kjeldahl and contain protein 154.5g in the high protein powder.
Measure as can be known by Kjeldahl, contain protein 16.5%, 165g in the former Grain Production of Amaranthus powder of seeds of every 1000g.Former Grain Production of Amaranthus powder of seeds obtains high protein powder 541.5g by said method, wherein contains protein 154.5g, and protein content is 28.56%, and the more former Grain Production of Amaranthus powder of seeds of protein content improves 77.1% in the high protein powder of embodiment 3 gained.
Grain Production of Amaranthus high protein powder of the present invention can be used as the raw material of making the high protein healthy food.
Claims (3)
1. the method for a preparing grain amaranth high protein powder by double enzyme process is characterized in that, comprises the steps:
(1) enzymolysis: with Grain Production of Amaranthus powder of seeds and water is 1: 40 preparation feed liquid by weight, after water bath with thermostatic control is warming up to 45～55 ℃, adjusts material liquid pH value to 4.5～5.0 backs and adds cellulase, reaction 2～4h; The pH value of feed liquid behind the cellulase degradation is adjusted to 6.0～7.0 back adding AMSs, at 50～60 ℃ of reaction 1～3h; Again with the reactant liquor behind twice enzymolysis at 80 ℃ of water-baths enzyme 30min that goes out; Wherein, the enzyme concentration of cellulase is the 20.8U/g substrate, the enzyme concentration 6.6U/g substrate of AMS;
(2) precipitation, centrifugal, dry: the reactant liquor adjust pH behind the enzyme that will go out to isoelectric point 4.6 makes the soluble protein precipitation; The centrifugal 20min of 3000r/min abandons supernatant afterwards, gets precipitation; With being deposited in after centrifugal-55 ℃ freeze drying 48h, promptly make the Grain Production of Amaranthus high protein powder.
2. the method for preparing grain amaranth high protein powder by double enzyme process according to claim 1, it is characterized in that, be 1: 40 preparation feed liquid with Grain Production of Amaranthus powder of seeds and water, after water bath with thermostatic control is warming up to 50 ℃ by weight, adjust material liquid pH value to 4.8 back and add the cellulase powder, reaction 3h; The pH value of the feed liquid behind the cellulase degradation is adjusted to 6.5 back adding AMS powder, at 55 ℃ of reaction 2h.
3. the method for preparing grain amaranth high protein powder by double enzyme process according to claim 1, it is characterized in that, in the step (1), working concentration is the HCl solution of 1mol/L when adjusting material liquid pH value, working concentration is the NaOH solution of 1mol/L when regulating the pH value of feed liquid behind the cellulase degradation, and working concentration was the HCl solution of 1mol/L when enzyme afterreaction liquid pH value was gone out in adjusting in the step (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010250995 CN101912029A (en) | 2010-08-11 | 2010-08-11 | Method for preparing grain amaranth high protein powder by double enzyme process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010250995 CN101912029A (en) | 2010-08-11 | 2010-08-11 | Method for preparing grain amaranth high protein powder by double enzyme process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101912029A true CN101912029A (en) | 2010-12-15 |
Family
ID=43319822
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010250995 Pending CN101912029A (en) | 2010-08-11 | 2010-08-11 | Method for preparing grain amaranth high protein powder by double enzyme process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101912029A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CZ304782B6 (en) * | 2013-02-22 | 2014-10-15 | Výzkumný ústav potravinářský Praha, v.v.i. | Debittered concentrate of amaranth proteins with increased amount of calcium and magnesium as well as process for preparing thereof |
| WO2014165490A3 (en) * | 2013-04-02 | 2015-03-19 | Air Products And Chemicals, Inc. | Compositions of delivery systems for personal care products |
| CN107841520A (en) * | 2017-10-31 | 2018-03-27 | 湖北省农业科学院农产品加工与核农技术研究所 | A kind of Grain Production of Amaranthus polypeptide product and preparation method and application |
| CN111454995A (en) * | 2020-04-08 | 2020-07-28 | 华中农业大学 | Method for promoting biomass material to produce alcohol |
| CN115815094A (en) * | 2022-12-10 | 2023-03-21 | 浙江南龙皮业有限公司 | Anti-cracking coating agent for leather and leather coating method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1478409A (en) * | 2003-07-11 | 2004-03-03 | 黄玉霖 | Polypeptide health-care nutritive food and its making method |
| CN1509625A (en) * | 2002-12-25 | 2004-07-07 | 北京市粮食科学研究所 | Enzymolysized modified vegetable protein |
| CN101077118A (en) * | 2007-06-12 | 2007-11-28 | 江南大学 | Method for preparing soluble heart trefoil protein |
-
2010
- 2010-08-11 CN CN 201010250995 patent/CN101912029A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1509625A (en) * | 2002-12-25 | 2004-07-07 | 北京市粮食科学研究所 | Enzymolysized modified vegetable protein |
| CN1478409A (en) * | 2003-07-11 | 2004-03-03 | 黄玉霖 | Polypeptide health-care nutritive food and its making method |
| CN101077118A (en) * | 2007-06-12 | 2007-11-28 | 江南大学 | Method for preparing soluble heart trefoil protein |
Non-Patent Citations (2)
| Title |
|---|
| 《中国酿造》 20071231 陶兴无等 大米渣蛋白提纯及增溶工艺研究 第14-16页 1-3 , 第8期 2 * |
| 《农业工程学报》 20030131 徐明生等 籽粒苋蛋白质的提取研究 第43-45页 1-3 第19卷, 第1期 2 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CZ304782B6 (en) * | 2013-02-22 | 2014-10-15 | Výzkumný ústav potravinářský Praha, v.v.i. | Debittered concentrate of amaranth proteins with increased amount of calcium and magnesium as well as process for preparing thereof |
| WO2014165490A3 (en) * | 2013-04-02 | 2015-03-19 | Air Products And Chemicals, Inc. | Compositions of delivery systems for personal care products |
| CN105101938A (en) * | 2013-04-02 | 2015-11-25 | 气体产品与化学公司 | Compositions of delivery systems for personal care products |
| CN107841520A (en) * | 2017-10-31 | 2018-03-27 | 湖北省农业科学院农产品加工与核农技术研究所 | A kind of Grain Production of Amaranthus polypeptide product and preparation method and application |
| CN111454995A (en) * | 2020-04-08 | 2020-07-28 | 华中农业大学 | Method for promoting biomass material to produce alcohol |
| CN111454995B (en) * | 2020-04-08 | 2021-09-24 | 华中农业大学 | Method for promoting biomass material to produce alcohol |
| CN115815094A (en) * | 2022-12-10 | 2023-03-21 | 浙江南龙皮业有限公司 | Anti-cracking coating agent for leather and leather coating method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101117352B (en) | Method for producing high temperature stable slow-slaking amidon and uses thereof | |
| CN111269770B (en) | Tartary buckwheat white spirit and brewing process thereof | |
| CN101912029A (en) | Method for preparing grain amaranth high protein powder by double enzyme process | |
| CN102696859A (en) | Method utilizing TG enzymatic modification to prepare isolated peanut protein | |
| CN103232522A (en) | Method for coproducing and preparing malt syrup and proteins from broken rice | |
| CN104004812A (en) | Glycosylation modification method of rice residue protein | |
| CN106962590A (en) | A kind of method for preparing edible fungus protein powder | |
| CN101278733B (en) | Edible fungus paper type food product and method of producing the same | |
| CN110250316B (en) | Production process of quick-dissolving soybean protein isolate | |
| CN111671018A (en) | Preparation method of Maillard reaction modified corn germ protein beverage | |
| CN104921243A (en) | Preparation method of alfalfa leaf protein peptide beverage | |
| CN103719702A (en) | Infant rice flour and preparation method thereof | |
| CN103250674A (en) | Method for raising ducks by sugarcane | |
| CN105076903A (en) | Camellia oil and grifola frondosa tapioca ball and preparation method thereof | |
| CN102860453A (en) | Waxy corn flour and preparation process thereof | |
| CN111772094A (en) | Low-GI instant bean jelly and preparation method thereof | |
| CN103757081A (en) | Industrialized production method of oat protein hydrolysate | |
| CN102038264B (en) | Preparation method for agaricus bisporus enzymatic hydrolysis peptide oral liquid | |
| CN103636916A (en) | Method for preparing high content rice bran protein from degreased rice bran | |
| CN111387448A (en) | Preparation method of high-elasticity vegetarian food | |
| CN103749978B (en) | Refined maize protein powder and preparation method thereof | |
| CN105286022A (en) | Compound protein powder rich in content of essential amino acids and reasonable in composition and preparation method of compound protein powder | |
| CN107712531A (en) | A kind of preparation method of highland barley enzymolysis liquid | |
| CN107018952A (en) | The cultural method of goat | |
| CN107581616A (en) | A kind of method that protein from lentinus edodes and mushroom oligosaccharide are synchronously prepared using mushroom |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20101215 |