CN107841520A - A kind of Grain Production of Amaranthus polypeptide product and preparation method and application - Google Patents
A kind of Grain Production of Amaranthus polypeptide product and preparation method and application Download PDFInfo
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- CN107841520A CN107841520A CN201711069529.9A CN201711069529A CN107841520A CN 107841520 A CN107841520 A CN 107841520A CN 201711069529 A CN201711069529 A CN 201711069529A CN 107841520 A CN107841520 A CN 107841520A
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- Prior art keywords
- amaranthus
- preparation
- grain production
- polypeptide
- extract solution
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 73
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 71
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 64
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 47
- 241000219318 Amaranthus Species 0.000 title claims abstract description 42
- 238000002360 preparation method Methods 0.000 title claims abstract description 38
- 239000002537 cosmetic Substances 0.000 claims abstract description 10
- 235000013402 health food Nutrition 0.000 claims abstract description 8
- 239000000047 product Substances 0.000 claims description 50
- 239000000284 extract Substances 0.000 claims description 40
- 102000004190 Enzymes Human genes 0.000 claims description 26
- 108090000790 Enzymes Proteins 0.000 claims description 26
- 229940088598 enzyme Drugs 0.000 claims description 24
- 239000006228 supernatant Substances 0.000 claims description 21
- 239000004365 Protease Substances 0.000 claims description 20
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 108091005804 Peptidases Proteins 0.000 claims description 10
- 108090000526 Papain Proteins 0.000 claims description 8
- 235000019834 papain Nutrition 0.000 claims description 8
- 229940055729 papain Drugs 0.000 claims description 8
- 235000019419 proteases Nutrition 0.000 claims description 8
- 239000007836 KH2PO4 Substances 0.000 claims description 7
- 108010022999 Serine Proteases Proteins 0.000 claims description 7
- 102000012479 Serine Proteases Human genes 0.000 claims description 7
- 101710097834 Thiol protease Proteins 0.000 claims description 7
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 7
- 101000829189 Staphylococcus aureus Glutamyl endopeptidase Proteins 0.000 claims description 6
- 108090001109 Thermolysin Proteins 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 102000005741 Metalloproteases Human genes 0.000 claims description 5
- 108010006035 Metalloproteases Proteins 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- 108010004032 Bromelains Proteins 0.000 claims description 4
- 235000019835 bromelain Nutrition 0.000 claims description 4
- 210000001913 submandibular gland Anatomy 0.000 claims description 4
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims description 3
- 210000000496 pancreas Anatomy 0.000 claims description 3
- 229910000397 disodium phosphate Inorganic materials 0.000 claims description 2
- 240000001592 Amaranthus caudatus Species 0.000 claims 1
- 235000009328 Amaranthus caudatus Nutrition 0.000 claims 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
- 235000012735 amaranth Nutrition 0.000 claims 1
- 239000004178 amaranth Substances 0.000 claims 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 abstract description 10
- 108090000623 proteins and genes Proteins 0.000 abstract description 10
- 238000006243 chemical reaction Methods 0.000 abstract description 8
- 238000000034 method Methods 0.000 abstract description 6
- 239000000243 solution Substances 0.000 description 33
- 230000007062 hydrolysis Effects 0.000 description 10
- 238000006460 hydrolysis reaction Methods 0.000 description 10
- 102000035195 Peptidases Human genes 0.000 description 9
- 235000018102 proteins Nutrition 0.000 description 9
- 235000001014 amino acid Nutrition 0.000 description 6
- 239000012530 fluid Substances 0.000 description 6
- 108090000317 Chymotrypsin Proteins 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 5
- 229960002376 chymotrypsin Drugs 0.000 description 5
- 235000013305 food Nutrition 0.000 description 5
- 239000001103 potassium chloride Substances 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 125000000539 amino acid group Chemical group 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 3
- 239000004472 Lysine Substances 0.000 description 3
- -1 aromatic L-amino acid Chemical class 0.000 description 3
- 230000035558 fertility Effects 0.000 description 3
- 230000002779 inactivation Effects 0.000 description 3
- 238000005374 membrane filtration Methods 0.000 description 3
- 239000011259 mixed solution Substances 0.000 description 3
- 240000003147 Amaranthus hypochondriacus Species 0.000 description 2
- 235000011746 Amaranthus hypochondriacus Nutrition 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
- 230000001079 digestive effect Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 235000015363 Amaranthus cruentus Nutrition 0.000 description 1
- 241001532704 Azolla Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 108010038061 Chymotrypsinogen Proteins 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 108010059378 Endopeptidases Proteins 0.000 description 1
- 102000005593 Endopeptidases Human genes 0.000 description 1
- 108010013369 Enteropeptidase Proteins 0.000 description 1
- 102100029727 Enteropeptidase Human genes 0.000 description 1
- 108010062466 Enzyme Precursors Proteins 0.000 description 1
- 102000010911 Enzyme Precursors Human genes 0.000 description 1
- 206010063560 Excessive granulation tissue Diseases 0.000 description 1
- 102000009123 Fibrin Human genes 0.000 description 1
- 108010073385 Fibrin Proteins 0.000 description 1
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 1
- 108010001515 Galectin 4 Proteins 0.000 description 1
- 102100039556 Galectin-4 Human genes 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 206010061926 Purulence Diseases 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108010027252 Trypsinogen Proteins 0.000 description 1
- 102000018690 Trypsinogen Human genes 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 125000002252 acyl group Chemical group 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 125000003368 amide group Chemical group 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 239000011942 biocatalyst Substances 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 150000001721 carbon Chemical group 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- YQDHCCVUYCIGSW-LBPRGKRZSA-N ethyl (2s)-2-benzamido-5-(diaminomethylideneamino)pentanoate Chemical compound NC(=N)NCCC[C@@H](C(=O)OCC)NC(=O)C1=CC=CC=C1 YQDHCCVUYCIGSW-LBPRGKRZSA-N 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 229950003499 fibrin Drugs 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 210000001126 granulation tissue Anatomy 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 210000001819 pancreatic juice Anatomy 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000009941 weaving Methods 0.000 description 1
- 235000020795 whole food diet Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
Abstract
The invention provides a kind of Grain Production of Amaranthus polypeptide product and preparation method and application, belong to protein engineering field.A kind of preparation method of Grain Production of Amaranthus polypeptide provided by the invention, simple to operate, step is brief, and by gentle reaction condition, natural polypeptide product just can largely be made, have preferable application value；The polypeptide product prepared using the above method, can apply in more field, is especially applied in health food or cosmetics and skincare product is prepared, have higher economic value and application value.
Description
Technical field
The present invention relates to protein engineering field, in particular to a kind of Grain Production of Amaranthus polypeptide product and its preparation
Method and application.
Background technology
With constantly advancing for economy, social continuous progress, people gradually start the requirement to quality of the life
More and more higher；Also more and more higher, requirement of the people to wholefood, health products and cosmetics and skincare product are also more next for the income of people
It is higher.
As demand is increasing, and demand is also not kept pace with production, so needing a large amount of large-scale production systems
Standby natural polypeptide products.
The content of the invention
The first object of the present invention is to provide a kind of preparation method of Grain Production of Amaranthus polypeptide, and the preparation method is simple to operate,
Step is brief, and by gentle reaction condition, natural polypeptide product just can largely be made, have preferable application value.
The second object of the present invention is to provide a kind of polypeptide product.
The third object of the present invention is answered in the above-mentioned polypeptide product of offer in health food or cosmetics and skincare product is prepared
With.
In order to realize the above-mentioned purpose of the present invention, using following technical scheme：
A kind of preparation method of Grain Production of Amaranthus polypeptide, including：
Grain Production of Amaranthus raw material, which adds extract solution and ground, obtains homogenate, centrifuges homogenate and extracts supernatant, obtains supernatant
Extract solution；
Polypeptide product is obtained after filtering supernatant extract solution and with enzyme preparation hydrolysis supernatant extract solution.
A kind of polypeptide product, polypeptide product are made using the preparation method of Grain Production of Amaranthus polypeptide described above.
Above-mentioned polypeptide product is applied in health food or cosmetics and skincare product is prepared.
Compared with prior art, beneficial effects of the present invention are：A kind of preparation side of Grain Production of Amaranthus polypeptide provided by the invention
Method, simple to operate, step is brief, and by gentle reaction condition, natural polypeptide product just can largely be made, and has preferable
Application value；The polypeptide product prepared using the above method, can apply in more field, especially prepare health care
Applied in food or cosmetics and skincare product, there is higher economic value and application value.
Embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the present invention.It is unreceipted specific in embodiment
Condition person, the condition suggested according to normal condition or manufacturer are carried out.Agents useful for same or the unreceipted production firm person of instrument, it is
The conventional products that can be obtained by commercially available purchase.
A kind of Grain Production of Amaranthus polypeptide product of the embodiment of the present invention and preparation method and application is specifically described below.
Grain Production of Amaranthus, also known as prince's-feather, be the annual grain of Amaranthaceae Amaranthus (Amaranthus hypochondriacus L.),
Raise, dish dual-purpose type crop.250~350 centimetres of plant height, cane is upright, there is a wane, thick 3~5 centimetres, Dan Ye, alternate, obovate
Or ovate-elliptic.Coniform root system, main root is undeveloped, and lateral root is flourishing, and root system is huge, focuses mostly in 10~30 centimetres of soil layer
It is interior.
Grain Production of Amaranthus is annual herb plant, and more than 2 meters of plant height, stem is red or green, thick 4 cm of stem, Ye Zhi
Growth, petiole, seed are tiny, and thousand seeds weigh about 0.54 gram.Grain Production of Amaranthus is short-day plant, likes warm and moist weather, fertility
Phase requirement has enough illumination.It is less demanding to soil, but consumption fertility is more, it is not drought-enduring intolerant to shade.The per mu yield of medium fertility plot
5~7 tons of fresh grass, palatability is good, and nutritive value is high, and crude protein content is up to 2~4% in fresh grass, therefore someone claims Grain Production of Amaranthus
For " azolla filliculoidas "；The protein of Grain Production of Amaranthus, fat content are enriched, and gross protein value can reach respectively in cauline leaf and seed
17.7~27.1% and more than 30%.
A kind of preparation method of Grain Production of Amaranthus polypeptide, including：
Grain Production of Amaranthus raw material, which adds extract solution and ground, obtains homogenate, centrifuges homogenate and extracts supernatant, obtains supernatant
Extract solution；
Filtering supernatant extract solution simultaneously obtains polypeptide product with enzyme preparation hydrolysis supernatant extract solution.
Further, Grain Production of Amaranthus raw material is the seed of Grain Production of Amaranthus.
Because the protein content of the seed kernel of Grain Production of Amaranthus is higher, it is easier to prepares polypeptide product；Certainly can be with
Plant progress biochemical treatment from Grain Production of Amaranthus prepares the preparation of the biochemical materials such as polypeptide product.
Further, Na2HPO4,130-140mmol/L of extract solution including final concentration of 7-9mmol/L NaCl,
1.5-2.5mmol/L KH2PO4 and 2.5-2.9mmol/L KCl.
Above-mentioned buffer solution is prepared, extensive, main component Na is used in biochemical research2HPO4、KH2PO4、
NaCl and KCl, due to Na2HPO4And KH2PO4They have a two level dissociation, and the pH value range of buffering is very wide；It can be very good to maintain
The ionic equilibrium of solution, form a stable solution environmental.
Further, the condition of centrifugation is 8000-12000rpm, under the conditions of temperature is 0-4 DEG C, centrifuges 15-40min.
Further, enzyme preparation is at least one of serine protease, thiol protease and metalloproteinases；And
Staphylococcus aureus V8 protease.
Serine protease is a protease family, and their effect is the peptide bond being broken in macro-molecular protein, is made
Turn into small protein or polypeptide；It, which is activated, is realized by the amino acid residue change of one group of activated centre, they
Among necessarily to have one be serine (origin of its name).Serine protease passes through neighbouring amino acid residue chain, silk ammonia
Sour residue is activated in activated centre；Necleophilic reaction occurs for the hydroxyl and the carbon atom of peptide bond being activated；After peptide bond fracture, acyl group
On carbon be esterified, the nitrogen end of peptide bond can be released free；Hydrolysis, the carbon teminal product being connected with enzyme are released；React
Into.
Inside mammal, serine protease plays critically important role, is particularly digesting, blood coagulation and benefit
Body system aspects.
Thiol protease is that sulfydryl (cysteine) is contained in a kind of activated centre, and by sulfydryl catalyzing hydrolysis peptide bond
Proteolytic enzyme.
Metalloproteinases refers to the protease containing metal ion in activated centre.
Staphylococcus aureus V8 protease, from Staphylococcus aureus V8 strains, aspartic acid and glutamic acid can be cut off
The enzyme of carboxy-terminal peptide bond.
Further, serine protease is at least one in chymotrypsin, trypsase and submaxillary gland protease
Kind, thiol protease includes at least one of bromelain and papain, and metalloproteinases includes thermophilic bacteria protein
Enzyme.
Trypsase Trypsin (Parenzyme) be protease one kind, EC 3.4.4.4, be from ox, sheep, pig pancreas
A kind of serine protein hydrolase of dirty extraction.In vertebrate, worked as digestive ferment.It is trypsase in pancreas
Precursor trypsinogen be synthesized after, secrete as the composition of pancreatic juice, resolved into by the limitation of enterokinase, or trypsase
It is endopeptidase to activate trypsase, it can cut off the carboxyl side in lysine and arginine residues in polypeptide chain.It
Not only play digestive ferment, and before can also limiting and decomposing chymotrypsinogen, procarboxypeptidase, other enzymes such as phosphatide proenzyme
Body, play activation.Trypsase is the most strong protease of specificity, and in the amino acid range for determining protein, it turns into
Indispensable instrument.Trypsase is a kind of proteolytic enzyme, can be selectively in aminosal by lysine or smart ammonia
The peptide chain that is formed of carboxyl of acid, can digest and dissolve denatured protein, to unmodified protein without effect, accordingly, it is capable to make purulence,
Sputum, blood clot etc. decompose, are thinning, are easy to drainage and exclude, and accelerate surface of a wound purification, promote granulation tissue newborn, in addition with anti-
Inflammatory effect.
Chymotrypsin is roughly the same with trypsase；All category participates in gal4 amino acid metabolic enzyme, and a kind of water
Solve enzyme.
Submaxillary gland protease is also a kind of serine protease, can be broken arginic carbon teminal site, protein degradation matter.
Papain category thiol protease, the c-terminus of arginine and lysine in hydrolyzable proteins and peptides, and
Can selective hydrolysis those there is the amino acid of two carboxyls or the peptide bond of aromatic L-amino acid at the N- ends of peptide bond.
Papain is made up of a kind of single chain polypeptide, contains 212 amino acid residues.At least three amino acid residues are deposited
It is the activated centre position of enzyme, they are Cys25, His159 and Asp158 respectively.Papain is in medicine, food, drink
Material, chemical reagent, feed, weaving cosmetics etc. are all widely used, and DEVELOPMENT PROSPECT is wide.
Bromelain category thiol protease, the optimum pH to casein, hemoglobin, BAEE is 6~8, to gelatin
It is pH value 5.0.Enzymatic activity is suppressed by heavy metal.Water is slightly soluble in, insoluble in ethanol, acetone, chloroform and ether.Its selective hydrolysis alkali
Peptide chain on the carboxyl side of acidic amino acid (such as arginine) or aromatic amino acid (such as phenylalanine, tyrosine), selection
Property hydrolysis of fibrin, makes polypeptide be hydrolyzed to the effect of the peptides of low molecule amount, also hydrolyzed amide groups key and esters.
The outer literary fame of thermolysin is Thermolysin.Speed when characteristic is hydrolyse hydrophobic strong amino acid,
Contain Ca in activated centre2+And Zn2+。
Further, counted according to unit of enzyme activity, 1.8-2.5U enzyme preparation is added according to every gram of extract solution.
Enzyme can play catalytic action, dosage excessively causes as a kind of biocatalyst under conditions of a small amount of concentration
Waste, the very few reaction speed of dosage reduces, and is unfavorable for actual production needs.
Further, the working ph values of enzyme preparation are 3.5-8.1,35-55 DEG C of operating temperature.
Enzyme has the pH value and reaction temperature of an optimal reaction, but also can in non-optimal conditions when reaction
Play effectiveness.
A kind of polypeptide product, polypeptide product are made using the preparation method of above-mentioned Grain Production of Amaranthus polypeptide.
Above-mentioned polypeptide product is applied in health food or cosmetics and skincare product is prepared.
The polypeptide product being prepared is applied to and prepared in health food, the nutritive value of health food can be improved,
That improves human body digests and assimilates efficiency.
The polypeptide product being prepared is applied to and prepared in cosmetics and skincare product, the work of certain beautifying face and moistering lotion can be played
With.
The feature and performance of the present invention are described in further detail with reference to embodiments.
Embodiment 1
The present embodiment provides a kind of preparation method of Grain Production of Amaranthus polypeptide, and concrete operation step is as follows：
1.1 prepare extract solutions, Na in extract solution2HPO4The final concentration of final concentration of 140mmol/L of 7mmol/L, NaCl,
KH2PO4The final concentration of final concentration of 2.9mmol/L of 2.5mmol/L, KCl；
1.2 weigh the seed of 1kg Grain Production of Amaranthus, and are added in extract solution, and grind and obtain homogenate；And extract solution with
The weight ratio of seed is 2:1；
1.3 under the conditions of 4 DEG C, 12000rpm centrifugation 15min, take supernatant, obtain supernatant extract solution；
1.4 with 0.22 μm of membrane filtration supernatant extract solution, obtains filtered fluid；
In 1.5 filtered fluids obtained into step 1.4 chymotrypsin, Papain are added according to 1.8U/g extract solutions
Enzyme, thermolysin and staphylococcus aureus V8 protease；The pH value for adjusting mixed solution is 3.5, and temperature control exists
35℃；
1.6, which continuously ferment, digests hydrolysis and high-temperature inactivation enzyme, and polypeptide product is made.
Embodiment 2
The present embodiment provides a kind of preparation method of Grain Production of Amaranthus polypeptide, and concrete operation step is as follows：
1.1 prepare extract solutions, Na in extract solution2HPO4The final concentration of final concentration of 135mmol/L of 8mmol/L, NaCl,
KH2PO4The final concentration of final concentration of 2.7mmol/L of 2.0mmol/L, KCl；
1.2 weigh the seed of 1kg Grain Production of Amaranthus, and are added in extract solution, and grind and obtain homogenate；And extract solution with
The weight ratio of seed is 2:1；
1.3 under the conditions of 0 DEG C, 11000rpm centrifugation 20min, takes supernatant, obtains supernatant extract solution；
1.4 with 0.22 μm of membrane filtration supernatant extract solution, obtains filtered fluid；
In 1.5 filtered fluids obtained into step 1.4 according to every gram of extract solution be separately added into 2.1U chymotrypsin,
Trypsase, papain, thermolysin and staphylococcus aureus V8 protease；Adjust the pH of mixed solution
It is worth for 5.5, temperature control is at 42 DEG C；
1.6, which continuously ferment, digests hydrolysis and high-temperature inactivation enzyme, and polypeptide product is made.
Embodiment 3
The present embodiment provides a kind of preparation method of Grain Production of Amaranthus polypeptide, and concrete operation step is as follows：
1.1 prepare extract solutions, Na in extract solution2HPO4The final concentration of final concentration of 130mmol/L of 9mmol/L, NaCl,
KH2PO4The final concentration of final concentration of 2.5mmol/L of 1.5mmol/L, KCl；
1.2 weigh the seed of 1kg Grain Production of Amaranthus, and are added in extract solution, and grind and obtain homogenate；And extract solution with
The weight ratio of seed is 2:1；
1.3 under the conditions of 3 DEG C, 8000rpm centrifugation 40min, take supernatant, obtain supernatant extract solution；
1.4 with 0.22 μm of membrane filtration supernatant extract solution, obtains filtered fluid；
In 1.5 filtered fluids obtained into step 1.4 according to every gram of extract solution be separately added into 2.5U chymotrypsin,
Trypsase, submaxillary gland protease, bromelain, papain, thermolysin and staphylococcus aureus V8
Protease；The pH value for adjusting mixed solution is 8.1, and temperature control is at 55 DEG C；
1.6, which continuously ferment, digests hydrolysis and high-temperature inactivation enzyme, and polypeptide product is made.
Embodiment 4
The present embodiment provides a kind of polypeptide product, and the polypeptide product that the present embodiment provides uses the preparation that embodiment 1 provides
Method is made.
And the polypeptide product for obtaining the present embodiment is applied to prepare health food or cosmetics and skincare product.
Experimental example 1
This experimental example is analyzed the embodiment 1-3 polypeptide products prepared, and specific method is as follows：
Example 1-3 prepares polypeptide product measure protein content, reference respectively《National food safety standard-food adds
Work vegetable protein》GB20371-2016, and the measure of national food safety standard-Protein in Food》GB5009.5-
2016 Kjeldahl's method, determine content of peptides in thick get sample product and concentrating sample.Testing result such as table 1.
The content of peptides testing result of table 1
| The thick product content of peptides (wt%) that gets sample | Concentrating sample content of peptides (wt%) | |
| Embodiment 1 | 43.8% | 79.2% |
| Embodiment 2 | 46.3% | 84.3% |
| Embodiment 3 | 44.6% | 82.1% |
The polypeptide product that as can be seen from Table 1 prepared by embodiment 1-3 it is thick get sample product and concentrating sample content of peptides it is big
In 40%, meet standard.
In summary, the preparation method of Grain Production of Amaranthus polypeptide provided in an embodiment of the present invention can by simple preparation process,
Gentle reaction condition prepares polypeptide product, and the obtained polypeptide product prepared has higher use value, and applies
Scope is wider；With higher practicality and higher application value.
Embodiments described above is part of the embodiment of the present invention, rather than whole embodiments.The reality of the present invention
The detailed description for applying example is not intended to limit the scope of claimed invention, but is merely representative of the selected implementation of the present invention
Example.Based on the embodiment in the present invention, what those of ordinary skill in the art were obtained under the premise of creative work is not made
Every other embodiment, belongs to the scope of protection of the invention.
Claims (10)
- A kind of 1. preparation method of Grain Production of Amaranthus polypeptide, it is characterised in that including：Grain Production of Amaranthus raw material, which adds extract solution and ground, obtains homogenate, centrifuges the homogenate and extracts supernatant, obtains supernatant Extract solution；Filter after the supernatant extract solution and hydrolyze the supernatant extract solution with enzyme preparation and obtain polypeptide product.
- 2. the preparation method of Grain Production of Amaranthus polypeptide according to claim 1, it is characterised in that the Grain Production of Amaranthus raw material is seed The seed of amaranth.
- 3. the preparation method of Grain Production of Amaranthus polypeptide according to claim 1, it is characterised in that the extract solution includes final concentration For 7-9mmol/L Na2HPO4, 130-140mmol/L NaCl, 1.5-2.5mmol/L KH2PO4And 2.5-2.9mmol/L KCl.
- 4. the preparation method of Grain Production of Amaranthus polypeptide according to claim 1, it is characterised in that the condition of the centrifugation is 8000-12000rpm, under the conditions of temperature is 0-4 DEG C, centrifuge 15-40min.
- 5. the preparation method of Grain Production of Amaranthus polypeptide according to claim 1, it is characterised in that the enzyme preparation is serine egg At least one of white enzyme, thiol protease and metalloproteinases；And staphylococcus aureus V8 protease.
- 6. the preparation method of Grain Production of Amaranthus polypeptide according to claim 5, it is characterised in that the serine protease is pancreas At least one of chrymotrypsin, trypsase and submaxillary gland protease, the thiol protease include bromelain and At least one of papain, the metalloproteinases include thermolysin.
- 7. the preparation method of Grain Production of Amaranthus polypeptide according to claim 5, it is characterised in that count, press according to unit of enzyme activity According to the enzyme preparation of extract solution addition 1.8-2.5U every gram described.
- 8. the preparation method of Grain Production of Amaranthus polypeptide according to claim 5, it is characterised in that the working ph values of the enzyme preparation For 3.5-8.1,35-55 DEG C of operating temperature.
- 9. a kind of polypeptide product, it is characterised in that the polypeptide product is using the Grain Production of Amaranthus polypeptide as described in claim 1-8 Preparation method is made.
- 10. polypeptide product as claimed in claim 9 is applied in health food or cosmetics and skincare product is prepared.
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| CN111454995A (en) * | 2020-04-08 | 2020-07-28 | 华中农业大学 | Method for promoting biomass material to produce alcohol |
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Application publication date: 20180327 |