CN113519857A - Acer truncatum peptide powder rich in nervonic acid and preparation method thereof - Google Patents
Acer truncatum peptide powder rich in nervonic acid and preparation method thereof Download PDFInfo
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- 241000219226 Acer truncatum Species 0.000 title claims abstract description 81
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 67
- GWHCXVQVJPWHRF-KTKRTIGZSA-N (15Z)-tetracosenoic acid Chemical compound CCCCCCCC\C=C/CCCCCCCCCCCCCC(O)=O GWHCXVQVJPWHRF-KTKRTIGZSA-N 0.000 title claims abstract description 64
- XJXROGWVRIJYMO-SJDLZYGOSA-N Nervonic acid Natural products O=C(O)[C@@H](/C=C/CCCCCCCC)CCCCCCCCCCCC XJXROGWVRIJYMO-SJDLZYGOSA-N 0.000 title claims abstract description 64
- GWHCXVQVJPWHRF-UHFFFAOYSA-N cis-tetracosenoic acid Natural products CCCCCCCCC=CCCCCCCCCCCCCCC(O)=O GWHCXVQVJPWHRF-UHFFFAOYSA-N 0.000 title claims abstract description 64
- 239000000843 powder Substances 0.000 title claims abstract description 59
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 238000003756 stirring Methods 0.000 claims abstract description 41
- 238000000034 method Methods 0.000 claims abstract description 28
- 239000004365 Protease Substances 0.000 claims abstract description 24
- 108090000526 Papain Proteins 0.000 claims abstract description 19
- 229940055729 papain Drugs 0.000 claims abstract description 19
- 235000019834 papain Nutrition 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 108010059892 Cellulase Proteins 0.000 claims abstract description 15
- 108010059820 Polygalacturonase Proteins 0.000 claims abstract description 13
- 229940106157 cellulase Drugs 0.000 claims abstract description 13
- 108010093305 exopolygalacturonase Proteins 0.000 claims abstract description 13
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 239000006228 supernatant Substances 0.000 claims abstract description 8
- 238000001035 drying Methods 0.000 claims abstract description 7
- 230000002255 enzymatic effect Effects 0.000 claims abstract description 4
- 239000000413 hydrolysate Substances 0.000 claims abstract description 4
- 238000000227 grinding Methods 0.000 claims abstract description 3
- 238000004108 freeze drying Methods 0.000 claims description 8
- 238000001694 spray drying Methods 0.000 claims description 6
- 238000000605 extraction Methods 0.000 abstract description 9
- 102000004169 proteins and genes Human genes 0.000 abstract description 8
- 108090000623 proteins and genes Proteins 0.000 abstract description 8
- 108091005804 Peptidases Proteins 0.000 abstract description 7
- 230000000415 inactivating effect Effects 0.000 abstract description 7
- 239000002994 raw material Substances 0.000 abstract description 6
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 235000019419 proteases Nutrition 0.000 abstract description 5
- 102000035195 Peptidases Human genes 0.000 abstract description 2
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 abstract description 2
- 238000002965 ELISA Methods 0.000 abstract 2
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- 235000019198 oils Nutrition 0.000 description 54
- 102000004190 Enzymes Human genes 0.000 description 21
- 108090000790 Enzymes Proteins 0.000 description 21
- 229940088598 enzyme Drugs 0.000 description 21
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 18
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- 206010039966 Senile dementia Diseases 0.000 description 2
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- 238000012360 testing method Methods 0.000 description 2
- 240000004144 Acer rubrum Species 0.000 description 1
- 235000011772 Acer rubrum var tomentosum Nutrition 0.000 description 1
- 235000009057 Acer rubrum var tridens Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 208000007443 Neurasthenia Diseases 0.000 description 1
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- 206010003549 asthenia Diseases 0.000 description 1
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- 210000002569 neuron Anatomy 0.000 description 1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
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- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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Abstract
The invention provides a acer truncatum peptide powder rich in nervonic acid and a preparation method thereof, wherein the method comprises the following steps: s1, grinding the acer truncatum oil residues, adding water to prepare paste, and adjusting the pH value to 5.5; s2, adding pectinase and cellulase into the solution of S1, and stirring for 2 hours at 55 ℃; adding papain, and stirring at 55 deg.C for 1-2 hr to obtain enzymatic hydrolysate; s3, centrifuging the enzymolysis liquid, taking supernatant, stirring for 1h at 100 ℃, and then drying to obtain the enzyme-linked immunosorbent assay (ELISA) inhibitor. The invention also provides the acer truncatum bunge peptide powder rich in nervonic acid obtained by the method. The method mainly uses acer truncatum oil residues as raw materials, adds three kinds of proteolytic enzymes, hydrolyzes protein in the oil residues into peptide powder at proper pH and temperature, heats and stirs, and fully combines the peptide powder and oil to form an oil-in-water structure while inactivating protease. The method has the advantages of obvious extraction effect, high nervonic acid content, easily-accessible raw materials of Acer Truncatum Bunge oil residue, low cost, and suitability for large-scale extraction and production.
Description
Technical Field
The invention relates to the technical field of acer truncatum bunge peptide powder, in particular to acer truncatum bunge peptide powder rich in nervonic acid and a preparation method thereof.
Background
Nervonic acid is divided into animal nervonic acid and plant nervonic acid, and human cranial nervonic acid belongs to animal nervonic acid, is a core natural component of cranial nerve cells and nerve tissues, and can promote the repair and regeneration of damaged nerve cells and tissues. However, the nervonic acid is difficult to synthesize in a human body by self and can only be supplemented by in vitro ingestion, and the ingestion of the nervonic acid can repair and dredge nerve fibers which are damaged brain nerve pathways, has great effects on improving the activity degree of the brain nerves and preventing the brain nerve aging, and also can play a good role in preventing senile dementia such as Parkinson's disease, Alzheimer's Disease (AD) and the like caused by neurodegeneration and neurasthenia.
Nervonic acid was originally derived from shark oil, however, the transient killing resulted in a dramatic reduction in the number of sharks. Therefore, in the process of searching other sources, people find that nervonic acid is rich in certain fruit and seed oil, which provides a new path for the research and development of nervonic acid. The research shows that the seeds of the acer truncatum bunge contain higher nervonic acid which is the nervonic acid source with the most development prospect at present, the oil content of the seeds can reach about 42 percent, and the content of the nervonic acid in the oil can reach more than 5 percent. Although acer truncatum is one of the most main sources for developing nervonic acid at present, the total amount is too small, and acer truncatum seed oil is only dozens of tons in the country, so the supply-demand ratio is greatly different. At present, the oil residue of acer truncatum seed oil is generally used as feed, and about 7% of oil still remains in the oil residue of acer truncatum seed oil, which causes great waste. Therefore, the invention develops a new method for preparing the acer truncatum bunge peptide powder rich in nervonic acid by using the acer truncatum bunge oil residues.
Disclosure of Invention
In order to solve the problems, the invention aims to provide a preparation method of acer truncatum bunge peptide powder rich in nervonic acid, which mainly hydrolyzes a large amount of protein contained in acer truncatum bunge oil residues by using protease to form peptide powder, heats and stirs the peptide powder and residual oil released in the hydrolysis process to enable the peptide powder and the oil to form emulsion, and prepares the solid acer truncatum bunge peptide powder rich in nervonic acid through drying treatment.
In order to achieve the above object, the technical solution of the present invention is as follows.
A preparation method of acer truncatum peptide powder rich in nervonic acid comprises the following steps:
s1, grinding the acer truncatum oil residues, adding water to prepare paste, and adjusting the pH value to 5.5;
s2, adding pectinase and cellulase into the solution of S1, and stirring for 2 hours at 55 ℃; adding papain, and stirring at 55 deg.C for 1-2 hr to obtain enzymatic hydrolysate;
s3, centrifuging the enzymolysis liquid of S2, taking supernatant, stirring for 1h at 100 ℃, and then drying to obtain the acer truncatum bunge peptide powder rich in nervonic acid.
Further, in S1, the dosage ratio of Acer truncatum oil residue to water is 1 g: 5-10 mL. Wherein, when the water consumption is reduced, the solution is too thick and difficult to stir, and when the water consumption is increased, the required enzyme amount is increased, and the economic cost is increased. Preferably, the dosage ratio of the acer truncatum oil residue to the water is 1 g: 7.5 mL.
Further, in S2, the mass ratio of the acer truncatum buge oil residue to the pectinase, the cellulase and the papain is 100: 0.5-1: 0.5-1: 0.5 to 1.
Further, in S3, the stirring speed was 1000 rpm.
Further, in S3, the operation of the drying process is as follows:
distilling the solution obtained by stirring for 1 hour at 100 ℃ under reduced pressure at 50-60 ℃ to obtain a concentrated solution; and (3) freeze-drying or spray-drying the concentrated solution to obtain the acer truncatum peptide powder rich in nervonic acid. Wherein the mass ratio of the solution obtained by stirring for 1h at 100 ℃ to the concentrated solution is about 5: 1. the temperature of the spray outlet of the spray drying was controlled at 105 ℃, and when the temperature was high, the saccharides contained in the peptide solution were carbonized, and the color was deepened.
The invention also provides the acer truncatum bunge peptide powder rich in nervonic acid, which is prepared by adopting the method.
The invention has the beneficial effects that:
1. the invention mainly adopts a hydrolytic enzyme method, and utilizes the means of mechanical crushing and enzymolysis to destroy and degrade cell walls in acer truncatum oil residues, so that cellulose and protein in the acer truncatum oil residues are hydrolyzed, residual oil is released, and under the action of high-temperature stirring, the peptide powder is promoted to be combined with the oil to obtain the acer truncatum peptide powder rich in nervonic acid. The method improves the extraction rate, can develop a new market of the acer truncatum peptide powder, reasonably recycles the oil residue of the acer truncatum, further improves the economic value of the acer truncatum oil residue, and can also provide a new health-care product for people.
2. The method of the invention uses acer truncatum oil residue rich in nervonic acid as a raw material, adds three kinds of proteolytic enzymes, hydrolyzes protein in the oil residue into peptide powder at proper pH and temperature, heats and stirs, fully combines the peptide powder with oil in the oil residue while inactivating protease to form an oil-in-water structure, prepares peptide powder solution rich in nervonic acid oil, and then prepares peptide powder rich in nervonic acid by freeze drying or spray drying. The method has the advantages of obvious extraction effect, high nervonic acid content, easily-obtained raw material of acer truncatum oil residue, low cost and suitability for large-scale extraction and production. The acer truncatum peptide powder rich in nervonic acid obtained by the invention can be used for developing health care products such as senile dementia, nerve repair and the like.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Pectinase, cellulase and papain are all purchased from Xian Dafengshou Biotech limited;
acer truncatum oil residues are purchased from Shanxi Red maple Biotech limited.
The experimental methods described in the following examples are all conventional methods unless otherwise specified; the reagents and materials are commercially available, unless otherwise specified.
The present invention will be further described with reference to the following specific examples, which are specifically shown in examples 1 to 4.
Example 1
A preparation method of acer truncatum peptide powder rich in nervonic acid comprises the following steps:
s1, selecting dry acer truncatum oil residues which are obtained by oil preparation through a cold pressing method and have no mildew.
Weighing 500g of acer truncatum oil residues, crushing, adding 3.75L of water, stirring into paste, and adjusting the pH value to 5.5 by using acetic acid;
s2, adding 2.5g of pectinase and 2.5g of cellulase into the solution of S1, and stirring for 2 hours at 55 ℃; then adding 2.5g of papain, and stirring for 2h at 55 ℃ to obtain an enzymolysis solution;
s3, centrifuging the enzymolysis liquid of S2 to remove precipitates, collecting supernatant, stirring at 100 ℃ for 1h at the rotating speed of 1000 r/min to inactivate the added enzyme, and completing the emulsification of the peptide and the grease while inactivating the enzyme. And then, a rotary evaporator is adopted to control the rotating speed to be 30-60 revolutions at the temperature of 50-60 ℃, and negative pressure evaporation is carried out for 2-3 hours to obtain concentrated solution with the concentration of about 5 times.
Placing the concentrated solution into a precooled freeze dryer, and preparing white instant acer truncatum bunge peptide powder rich in nervonic acid by adopting a freeze drying method.
Example 2
A preparation method of acer truncatum peptide powder rich in nervonic acid comprises the following steps:
s1, selecting dry acer truncatum oil residues which are obtained by oil preparation through a cold pressing method and have no mildew.
Weighing 500g of acer truncatum oil residues, crushing, adding 2.5L of water, stirring into paste, and adjusting the pH value to 5.5 by using acetic acid;
s2, adding 5g of pectinase and 5g of cellulase into the solution of S1, and stirring for 2 hours at 55 ℃; then adding 5g of papain, and stirring for 2h at 55 ℃ to obtain an enzymolysis solution;
s3, centrifuging the enzymolysis liquid of S2 to remove precipitates, collecting supernatant, stirring at 100 ℃ for 1h at the rotating speed of 1000 r/min to inactivate the added enzyme, and completing the emulsification of the peptide and the grease while inactivating the enzyme. And then, a rotary evaporator is adopted to control the rotating speed to be 30-60 revolutions at the temperature of 50-60 ℃, and negative pressure evaporation is carried out for 2-3 hours to obtain concentrated solution with the concentration of about 5 times.
Drying the concentrated solution at medium temperature by spray drying method, and controlling the temperature of the spray outlet at 105 deg.C to obtain Acer Truncatum Bunge peptide powder rich in nervonic acid. Wherein, when the temperature of the spray opening is higher than 105 ℃, the saccharides contained in the peptide liquid can be carbonized, so that the color of the product is deepened.
Example 3
A preparation method of acer truncatum peptide powder rich in nervonic acid comprises the following steps:
s1, selecting dry acer truncatum oil residues which are obtained by oil preparation through a cold pressing method and have no mildew.
Weighing 500g of acer truncatum oil residues, crushing, adding 3.5L of water, stirring into paste, and adjusting the pH value to 5.5 by using acetic acid;
s2, adding 3g of pectinase and 3g of cellulase into the solution of S1, and stirring for 2 hours at 55 ℃; then adding 3g of papain, and stirring for 2h at 55 ℃ to obtain an enzymolysis solution;
s3, centrifuging the enzymolysis liquid of S2 to remove precipitates, collecting supernatant, stirring at 100 ℃ for 1h at the rotating speed of 1000 r/min to inactivate the added enzyme, and completing the emulsification of the peptide and the grease while inactivating the enzyme. And then, a rotary evaporator is adopted to control the rotating speed to be 30-60 revolutions at the temperature of 50-60 ℃, and negative pressure evaporation is carried out for 2-3 hours to obtain concentrated solution with the concentration of about 5 times.
Placing the concentrated solution into a precooled freeze dryer, and preparing white instant acer truncatum bunge peptide powder rich in nervonic acid by adopting a freeze drying method.
Example 4
A preparation method of acer truncatum peptide powder rich in nervonic acid comprises the following steps:
s1, selecting dry acer truncatum oil residues which are obtained by oil preparation through a cold pressing method and have no mildew.
Weighing 500g of acer truncatum oil residues, crushing, adding 5L of water, stirring into paste, and adjusting the pH to 5.5 by using acetic acid;
s2, adding 3g of pectinase and 3g of cellulase into the solution of S1, and stirring for 2 hours at 55 ℃; then adding 3g of papain, and stirring for 2h at 55 ℃ to obtain an enzymolysis solution;
s3, centrifuging the enzymolysis liquid of S2 to remove precipitates, collecting supernatant, stirring at 100 ℃ for 1h at the rotating speed of 1000 r/min to inactivate the added enzyme, and completing the emulsification of the peptide and the grease while inactivating the enzyme. And then, a rotary evaporator is adopted to control the rotating speed to be 30-60 revolutions at the temperature of 50-60 ℃, and negative pressure evaporation is carried out for 2-3 hours to obtain concentrated solution with the concentration of about 5 times.
Placing the concentrated solution into a precooled freeze dryer, and preparing white instant acer truncatum bunge peptide powder rich in nervonic acid by adopting a freeze drying method.
Comparative example 1
The preparation method of the acer truncatum peptide powder rich in nervonic acid is basically the same as that of the example 1, and is different in that a series of screening tests are carried out on a single hydrolase and the action temperature and pH environment of the hydrolase, and the details are shown in Table 1.
TABLE 1 types of different hydrolases used and conditions of action
| Numbering | Raw materials | Temperature/. degree.C | pH |
| 1 | Pectin | 15、25、35、45、55 | 5.5 |
| 2 | Pectin | 55 | 2.5、3.5、4.5、5.5、6.0 |
| 3 | Cellulase enzymes | 45、55、65 | 5.5 |
| 4 | Cellulase enzymes | 55 | 4.0、5.0、5.5 |
| 5 | Papain | 50、55、60 | 5.5 |
| 6 | Papain | 55 | 5.0、5.5、6.0、6.5、7.0 |
Comparative example 2
A preparation method of acer truncatum peptide powder rich in nervonic acid is basically the same as that of example 1, except that,
s1, weighing 500g of acer truncatum oil residues, crushing, adding 3.75L of water, stirring into paste, and adjusting the pH value to 5.5 by using acetic acid.
S2, adding pectinase, cellulase and papain into the solution of the S1 in a mass ratio of 1: 1: 1, wherein 2.5g of pectinase, 2.5g of cellulase and 2.5g of papain are sequentially stirred at 55 ℃ for 2 hours to obtain an enzymatic hydrolysate.
Comparative example 3
A preparation method of acer truncatum peptide powder rich in nervonic acid comprises the following steps:
s1, weighing 500g of acer truncatum oil residues, crushing, adding 5L of water, stirring into paste, and adjusting the pH value to 5.5 by using acetic acid;
s2, adding 2.5g of pectinase and 2.5g of cellulase into the solution of S1, and standing for 2 hours at 55 ℃; then adding 2.5g of papain, and standing for 2h at 55 ℃ to obtain an enzymolysis solution;
s3, centrifuging the enzymolysis liquid of S2 to remove precipitates, collecting supernatant, stirring at 100 ℃ for 1h at the rotating speed of 1000 r/min to inactivate the added enzyme, and completing the emulsification of the peptide and the grease while inactivating the enzyme. And then, a rotary evaporator is adopted to control the rotating speed to be 30-60 revolutions at the temperature of 50-60 ℃, and negative pressure evaporation is carried out for 2-3 hours to obtain concentrated solution with the concentration of about 5 times.
Placing the concentrated solution into a precooled freeze dryer, and preparing white instant acer truncatum bunge peptide powder rich in nervonic acid by adopting a freeze drying method.
The extraction results of examples 1-4 are relatively close, so the results of example 1 are compared with comparative examples 1-3 below, and the results are as follows:
the screening test of comparative example 1 shows that the optimum reaction temperature and the optimum reaction pH value of the three enzymes are slightly different, but the difference between the pH values is not large between 5.0 and 5.5, the temperature difference is not obvious between 55 and 60 ℃, the pH value and the temperature are prevented from being adjusted in the production process for simplifying the production process, and the conditions of the hydrolytic protease in the peptide powder production process are determined to be consistent. Therefore, the reaction conditions for hydrolyzing the protease were set to pH 5.5 and temperature 55 ℃.
Example 1 used a stepwise hydrolysis of three enzymes, whereas comparative example 2 used a one-step addition hydrolysis of three enzymes. The step-by-step hydrolysis is adopted in the embodiment 1 of the invention, mainly because the papain is non-specifically degraded protein, and the method of the comparative example 2 can degrade other two enzymes by adding the three enzymes at one time, so that the activities of the other two enzymes are reduced. And the step-by-step addition of the embodiment 1, the first two enzymes are added before the papain, so that the influence of the papain can be avoided, the reduction of cellulose and pectin is completed, the pollen cell wall result is damaged, the protein and the grease in the pollen are easier to release, and the papain is added to degrade the protein to prepare the peptide powder. Comparative example 2 three enzymes were added at one time, and the extraction rate of peptide powder was about 10%. And in the embodiment 1, three enzymes are added step by step, and the extraction rate of the peptide powder is over 15 percent. Therefore, the method of example 1 of the present invention has a higher extraction rate of the nervonic acid oil from the acer truncatum oil residue, as compared with comparative example 2.
The oil content of the acer truncatum peptide powder containing nervonic acid obtained in example 1 and comparative example 3 was determined, and the results are shown in table 2.
TABLE 2 determination of the oil and fat content in acer truncatum peptide powder
| Conditions of hydrolysis | Oil content/%) | |
| Comparative example 3 | No stirring in the hydrolysis process | 8.9 |
| Example 1 | Stirring in the hydrolysis process | 25.1 |
The results in table 2 show that the oil and protein can be promoted to be released by stirring in the hydrolysis process, which is beneficial to the emulsification of the oil and the peptide and the increase of the content of the oil in the peptide powder.
The measurement results of nervonic acid in the peptide powder of acer truncatum bunge containing nervonic acid obtained in example 1 are shown in table 3.
TABLE 3 determination of nervonic acid in acer truncatum peptide powder
As can be seen from the results in tables 2 and 3, the content of nervonic acid in the acer truncatum peptide powder obtained in example 1 was 1.33%. Therefore, the content of the oil in the acer truncatum peptide powder prepared by the method exceeds 20%, the content of the nervonic acid in the oil exceeds 5%, namely the content of the nervonic acid in the acer truncatum peptide powder exceeds 1%. Can be used in health food for nerve rehabilitation or intelligence development.
In conclusion, the utilization rate of the acer truncatum buge oil residue is enhanced by extracting nervonic acid from the acer truncatum buge oil residue serving as a raw material through protein hydrolysis, acting through proper hydrolase at proper pH and temperature, performing a series of filtering and evaporation, and finally, preparing the solution rich in nervonic acid into powder peptide through freeze drying or spray drying, so that the content of the nervonic acid is detected, and the value of the acer truncatum buge oil residue is improved.
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. A preparation method of acer truncatum peptide powder rich in nervonic acid is characterized by comprising the following steps:
s1, grinding the acer truncatum oil residues, adding water to prepare paste, and adjusting the pH value to 5.5;
s2, adding pectinase and cellulase into the solution of S1, and stirring for 2 hours at 55 ℃; adding papain, and stirring at 55 deg.C for 1-2 hr to obtain enzymatic hydrolysate;
s3, centrifuging the enzymolysis liquid of S2, taking supernatant, stirring for 1h at 100 ℃, and then drying to obtain the acer truncatum bunge peptide powder rich in nervonic acid.
2. The method for preparing the acer truncatum bunge peptide powder rich in nervonic acid as claimed in claim 1, wherein in S1, the dosage ratio of acer truncatum bunge oil residue to water is 1 g: 5-10 mL.
3. The method for preparing the acer truncatum bunge peptide powder rich in nervonic acid as claimed in claim 1, wherein in S2, the mass ratio of the acer truncatum bunge oil residue to the pectinase, the cellulase and the papain is 100: 0.5-1: 0.5-1: 0.5 to 1.
4. The method for preparing the acer truncatum bunge peptide powder rich in nervonic acid as claimed in claim 1, wherein in S3, the stirring speed is 1000 r/min.
5. The method for preparing the acer truncatum bunge peptide powder rich in nervonic acid as claimed in claim 1, wherein in S3, the drying treatment is performed as follows:
distilling the solution obtained by stirring for 1 hour at 100 ℃ under reduced pressure at 50-60 ℃ to obtain a concentrated solution; and (3) freeze-drying or spray-drying the concentrated solution to obtain the acer truncatum peptide powder rich in nervonic acid.
6. A acer truncatum peptide powder rich in nervonic acid, which is characterized by being prepared by the method of any one of claims 1 to 5.
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