CN1478409A - Polypeptide health-care nutritive food and its making method - Google Patents

Polypeptide health-care nutritive food and its making method Download PDF

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Publication number
CN1478409A
CN1478409A CNA031473660A CN03147366A CN1478409A CN 1478409 A CN1478409 A CN 1478409A CN A031473660 A CNA031473660 A CN A031473660A CN 03147366 A CN03147366 A CN 03147366A CN 1478409 A CN1478409 A CN 1478409A
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polypeptide
healthful
nutriment
protein
manufacture method
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CN1276721C (en
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黄玉霖
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Spring Agriculture (xiamen) Biological Technology Co Ltd
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Abstract

A nutritive health-care polypeptide food is prepared from animal protein and plant protein through pulverizing plant protein, adding cellulase, controlling pH to 8-9, hydrolysis, homogenizing animal protein, proportional mixing, heating, cooling, adding proteinase, hydrolysis, regulating pH to 7, and vacuum heating for concentrating and drying. Its advantages are delicious taste, and rich nutrients.

Description

Polypeptide healthful nutriment and manufacture method thereof
(1) technical field
The present invention relates to a kind of main polypeptide food that passes through the bioengineering enzyme hydrolysis and preparation method thereof.
(2) background technology
Because peptide material has human body and promotes cardiovascular activity, regulates endocrine, improves nervous function, improves effect such as body immunity, the health nutrient that therefore contains peptide material obtains people's welcome.The CN1046191C patent provides a kind of polypeptide healthful nutriment, it contains bone peptone, meat peptone, animal embryo and placentapeptone, the Radix Astragali, dried orange peel and hawthorn, wherein the weight content of each is as follows: bone peptone 77%~85%, meat peptone 7%~15%, animal embryo and placentapeptone 0.5%~1.0%, the Radix Astragali 0.5%~1.0%, dried orange peel 0.2%~1.0%, hawthorn 2%~5%.Its preparation method comprises the boiling of poultry hectic fever due to yin is become grease removal after the colloid shape leachate, carries out enzymolysis with pancreatin, after the filtration filtrate is dried to powder; To raise meat and stir into meat gruel, carry out enzymolysis, after the filtration filtrate will be dried to Powdered with pancreas; With animal embryo with carry out enzymolysis with pancreas after placenta stirs into meat gruel, after the filtration filtrate is dried to Powdered; With the Radix Astragali, dried orange peel, hawthorn through pulverize, mix, soak, decoct immersion liquid, leave standstill, filter, concentrate, dry powder; All powder is mixed.Obviously, because hydrolase directly adopts animal pancreas directly to add in the hydrolyzate after crushed, therefore the active unit of enzyme and optimal pH value, temperature, time etc. all can't strict be controlled, cause unstable product quality, more can't directed control produce the content of certain Functional Polypeptides, can't debitterize.
(3) summary of the invention
The present invention aims to provide a kind of tasty mouthfeel, nutritious polypeptide healthful nutriment.
But another object of the present invention is to provide a kind of manufacture method of polypeptide healthful nutriment of constant product quality debitterize.
The said polypeptide healthful nutriment of the present invention contains animal protein, phytoprotein, and its content (mass ratio, down together) is animal protein: phytoprotein=1: (0.6~0.8).
Said animal protein can be selected from bone, muscle of at least a animal etc., and said phytoprotein can be selected from least a beans, marine alga, spirulina etc.
The processing step of the said polypeptide healthful nutriment manufacture method of the present invention is as follows:
1, plant protein material is pulverized, and adding cellulase, to control its pH value be 8~9, and temperature is 30~50 ℃ of hydrolysis cell membranes, must the hydrolyzed vegetable protein raw material;
2, animal protein raw material: aggregate is pulverized, and the even matter of muscle becomes rotten, adds the hydrolyzed vegetable protein raw material of step 1 gained, is heated to 70~100 ℃, makes protein denaturation, is liquid object;
3, liquid object is cooled to 35~50 ℃, regulating pH is 6~9, adds protease hydrolytic 2~5h;
4, adding acid for adjusting pH is 3~4, makes the insoluble substance precipitation, and hydrolyzate is transparence;
5, getting transparent hydrolyzate and add the solution of zinc sulfate stirring, whether have floccule precipitation, if then continue hydrolysis if observing; If not, then adding alkali adjusting pH is 7;
6, to be concentrated into solid contents be 25%~50% of total amount to heating in vacuum, and kill enzyme;
7, drying gets product.
In protein raw material is selected for use, can select animal protein: phytoprotein=1: (0.6~0.8).
In step 1, said cellulase can be chosen white ant cellulase.
In step 2, the cell membrane of said plant protein material is a polysaccharide, is hydrolyzed with wine brewing class mould, is heated to 70~100 ℃ again.
In step 3, said protease can be selected from trypsase, pepsin, papain or bromelain etc.The amount that adds may be selected to be (0.008~0.012) with the ratio of step 2 gained liquid object: 1.
In step 4, the said acid for adjusting pH value that adds, its acid can be inorganic acid or organic acid.
In step 7, said drying can adopt spray-drying or freeze drying.
Raw material of the present invention adopts animal protein and vegetable protein, especially animal bone, muscle and beans, marine alga, spirulina etc., make full use of in the form adding hydrolyzate of selectivity with complex enzyme and compound bacteria of enzyme, various enzymes and various bacterium are respectively executed its duty in hydrolyzate, as the cellulase pair cell wall broken wall that is hydrolyzed, various protease are hydrolyzed to protein simultaneously, various moulds utilize fermentation such as the zymolytic polysaccharide of cellulose to produce the 2-butanols and other fusel carries out debitterize to the bitter peptide that enzymolysis produces, thereby obtain tasty mouthfeel, nutritious peptide product.Secondly, the present invention follows the tracks of the hydrolysis performance level and detects and directed control, thereby obtains stabilized quality.Simultaneously, the present invention adopts multiple complex enzyme (especially using white ant cellulase) and compound fungus, makes to have two effects of broken wall and debitterize.
(4) specific embodiment
The present invention is further illustrated for following examples.
Embodiment 1: choose 6 kilograms of phytoprotein raw soybeans, pulverize earlier, add white ant cellulase, controlling its pH is 8.5, and temperature is controlled at 40~42 ℃ of hydrolysis cell membranes, gets the hydrolyzed vegetable protein raw material.With 10 kilograms of pulverizing of animal protein raw material ox bone, add the hydrolyzed vegetable protein raw material of above-mentioned gained, be heated to 90~95 ℃, making protein denaturation is liquid object.Liquid object is cooled to 35~38 ℃, and regulating pH is 8, adds protease hydrolytic 4h, and adding inorganic acid for adjusting pH after enzymolysis is finished again is 3.5, makes the insoluble substance precipitation, and hydrolyzate is transparence.Get transparent hydrolyzate and add solution of zinc sulfate and be stirred to no floccule precipitation, adding alkali, to regulate pH be 7, and to be concentrated into solid contents be 40%~45% of total amount to heating in vacuum again, and enzyme extremely, and spray-drying gets powder product.
Embodiment 2: choose 8 kilograms of phytoprotein raw soybeans, pulverize earlier, add white ant cellulase, controlling its pH is 8.8, and temperature is controlled at 48~50 ℃ of hydrolysis cell membranes, gets the hydrolyzed vegetable protein raw material.With 10 kilograms of pulverizing of animal protein raw material ox bone, add the hydrolyzed vegetable protein raw material of above-mentioned gained, be heated to 95~100 ℃, making protein denaturation is liquid object.Liquid object is cooled to 48~50 ℃, and regulating pH is 6, adds protease hydrolytic 2h, and adding inorganic acid for adjusting pH after hydrolysis is finished again is 3, makes the insoluble substance precipitation, and hydrolyzate is transparence.Get a little transparent hydrolyzate and add solution of zinc sulfate and be stirred to no floccule precipitation, adding alkali, to regulate pH be 7, and to be concentrated into solid contents for about 75 ℃ be 48%~50% of total amount to heating in vacuum again, and enzyme extremely, and spray-drying gets powder product.
Embodiment 3: choose 7 kilograms of phytoprotein raw soybeans, pulverize earlier, add white ant cellulase (or other cellulases), controlling its pH is 9, and temperature is controlled at 45~50 ℃ of hydrolysis cell membranes, gets the hydrolyzed vegetable protein raw material.With 10 kilograms of pulverizing of animal protein raw material ox bone, add the hydrolyzed vegetable protein raw material of above-mentioned gained, be heated to 70~75 ℃, making protein denaturation is liquid object.Liquid object is cooled to 40~45 ℃, and regulating pH is 8, adds protease hydrolytic 5h, and regulating pH is 8, and adding alkali adjusting pH again is 3.5, makes the insoluble substance precipitation, and hydrolyzate is transparence.Get transparent hydrolyzate and add solution of zinc sulfate and be stirred to no floccule precipitation, adding inorganic acid for adjusting pH is 7, and to be concentrated into solid contents be 45%~50% of total amount to heating in vacuum again, and enzyme extremely, and spray-drying gets powder product.
Embodiment 4: choose 7.5 kilograms of phytoprotein raw soybeans, pulverize earlier, add white ant cellulase, controlling its pH is 8, and temperature is controlled at 35~40 ℃ of hydrolysis cell membranes, gets the hydrolyzed vegetable protein raw material.With 10 kilograms of pulverizing of animal protein raw material ox bone, add the hydrolyzed vegetable protein raw material of above-mentioned gained, be heated to 100 ℃, making protein denaturation is liquid object.Liquid object is cooled to 45~50 ℃, and regulating pH is 7, adds protease hydrolytic 4h, and adding inorganic acid for adjusting pH again is 4, makes the insoluble substance precipitation, and hydrolyzate is transparence.Get transparent hydrolyzate and add solution of zinc sulfate and be stirred to no floccule precipitation, adding alkali, to regulate pH be 7, and to be concentrated into solid contents be 30%~35% of total amount to heating in vacuum again, and enzyme extremely, and spray-drying gets powder product.
Embodiment 5: choose 6.5 kilograms of phytoprotein raw soybeans, pulverize earlier, add white ant cellulase, controlling its pH is 8.4, and temperature is controlled at 30~35 ℃ of hydrolysis cell membranes, gets the hydrolyzed vegetable protein raw material.With 6 kilograms of pulverizing of animal protein raw material ox bone, 4 kilograms of even matter of beef become rotten, add the hydrolyzed vegetable protein raw material of above-mentioned gained, are heated to 80~85 ℃, and making protein denaturation is liquid object.Liquid object is cooled to 40~42 ℃, and regulating pH is 9, adds protease hydrolytic 3h, and adding inorganic acid for adjusting pH again is 3.5, makes the insoluble substance precipitation, and hydrolyzate is transparence.Get transparent hydrolyzate and add solution of zinc sulfate and be stirred to no floccule precipitation, adding alkali, to regulate pH be 7, and to be concentrated into solid contents be 25%~30% of total amount to heating in vacuum again, and enzyme extremely, and spray-drying gets powder product.
Embodiment 6: choose 7 kilograms of phytoprotein raw soybeans, pulverize earlier, add white ant cellulase (or other cellulases), controlling its pH is 8.5, and temperature is controlled at 40~42 ℃ of hydrolysis cell membranes, gets the hydrolyzed vegetable protein raw material.10 kilograms of even matter of animal protein raw material beef are become rotten, add the hydrolyzed vegetable protein raw material of above-mentioned gained, be heated to 85~90 ℃, making protein denaturation is liquid object.Liquid object is cooled to 35~40 ℃, and regulating pH is 7.5, adds protease hydrolytic 3.5h, and adding inorganic acid for adjusting pH again is 4, makes the insoluble substance precipitation, and hydrolyzate is transparence.Get transparent hydrolyzate and add solution of zinc sulfate and be stirred to no floccule precipitation, adding alkali, to regulate pH be 7, and to be concentrated into solid contents be 35%~40% of total amount to heating in vacuum again, and enzyme extremely, and spray-drying gets powder product.

Claims (10)

1, polypeptide healthful nutriment is characterized in that containing animal protein, phytoprotein, is animal protein by the content of its mass ratio: phytoprotein=1: 0.6~0.8.
2, polypeptide healthful nutriment as claimed in claim 1 is characterized in that said animal protein is selected from the bone of at least a animal, or bone and muscle.
3, polypeptide healthful nutriment as claimed in claim 1 is characterized in that said phytoprotein is selected from least a beans, marine alga or spirulina.
4, the manufacture method of polypeptide healthful nutriment is characterized in that its processing step is as follows:
1), plant protein material pulverizes, adding cellulase, to control its pH value be 8~9, temperature is 30~50 ℃ of hydrolysis cell membranes, must the hydrolyzed vegetable protein raw material;
2), animal protein raw material: aggregate is pulverized, and the even matter of muscle becomes rotten, adds the hydrolyzed vegetable protein raw material of step 1 gained, is heated to 70~100 ℃, makes protein denaturation, is liquid object;
3), liquid object is cooled to 35~50 ℃, regulating pH is 6~9, adds protease hydrolytic 2~5h;
4), to add acid for adjusting pH be 3~4, makes the insoluble substance precipitation, hydrolyzate is transparence;
5), get transparent hydrolyzate and add solution of zinc sulfate and stir, whether have floccule precipitation, if then continue hydrolysis if observing; If not, then adding alkali adjusting pH is 7;
6), to be concentrated into solid contents be 25%~50% of total amount to heating in vacuum, and kills enzyme;
7), drying, get product.
5, the manufacture method of polypeptide healthful nutriment as claimed in claim 4 is characterized in that in protein raw material is selected for use, selects animal protein: phytoprotein=1: 0.6~0.8.
6, the manufacture method of polypeptide healthful nutriment as claimed in claim 4 is characterized in that said cellulase chooses white ant cellulase.
7, the manufacture method of polypeptide healthful nutriment as claimed in claim 4, the cell membrane that it is characterized in that said plant protein material in step 2 is a polysaccharide, is hydrolyzed with wine brewing class mould, heats 70~100 ℃ again.
8, the manufacture method of polypeptide healthful nutriment as claimed in claim 4 is characterized in that said hydrolysising protease is selected from trypsase, pepsin, papain or bromelain.
9, the manufacture method of polypeptide healthful nutriment as claimed in claim 4 is characterized in that the amount that said hydrolysising protease adds and the ratio of step 2 gained liquid object are chosen as 0.008~0.012: 1.
10, the manufacture method of polypeptide healthful nutriment as claimed in claim 4 is characterized in that the said acid for adjusting pH value that adds, and its acid is inorganic acid or organic acid.
CN 03147366 2003-07-11 2003-07-11 Polypeptide health-care nutritive food and its making method Expired - Fee Related CN1276721C (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101912029A (en) * 2010-08-11 2010-12-15 天津商业大学 Method for preparing grain amaranth high protein powder by double enzyme process
CN103005378A (en) * 2012-12-21 2013-04-03 中国肉类食品综合研究中心 Novel low-sodium salt substitute and preparation method thereof
CN103431286A (en) * 2013-09-01 2013-12-11 陆思烨 Bullacta polypeptide nutrient and health-protection steamed bun and processing method thereof
CN103919132A (en) * 2014-04-24 2014-07-16 邱银平 Preparation method of ant powder containing ant polypeptides and chitosan
CN110172491A (en) * 2019-04-30 2019-08-27 世堃堂(广东)生物科技有限公司 A kind of multi-element biologic protein peptides and preparation method thereof
CN114631592A (en) * 2022-04-13 2022-06-17 广西弘山堂生物科技有限公司 Composite short peptide nutrient and preparation method thereof

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101912029A (en) * 2010-08-11 2010-12-15 天津商业大学 Method for preparing grain amaranth high protein powder by double enzyme process
CN103005378A (en) * 2012-12-21 2013-04-03 中国肉类食品综合研究中心 Novel low-sodium salt substitute and preparation method thereof
CN103431286A (en) * 2013-09-01 2013-12-11 陆思烨 Bullacta polypeptide nutrient and health-protection steamed bun and processing method thereof
CN103919132A (en) * 2014-04-24 2014-07-16 邱银平 Preparation method of ant powder containing ant polypeptides and chitosan
CN103919132B (en) * 2014-04-24 2015-10-21 邱银平 Containing the preparation method of the ant powder of ant polypeptide, shitosan
CN110172491A (en) * 2019-04-30 2019-08-27 世堃堂(广东)生物科技有限公司 A kind of multi-element biologic protein peptides and preparation method thereof
CN114631592A (en) * 2022-04-13 2022-06-17 广西弘山堂生物科技有限公司 Composite short peptide nutrient and preparation method thereof

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Owner name: QUANNONG ( XIAMEN ) BIOLOGY SCIENCE AND TECHNOLOGY

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Address after: Room E705, Taiwan science and technology enterprise incubation center, Xiamen torch hi tech Zone (Xiangan), Fujian, China

Patentee after: Spring agriculture (Xiamen) Biological Technology Co., Ltd.

Address before: Haibin 22-602, Xiamen University, Fujian, Xiamen

Patentee before: Huang Yulin

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Granted publication date: 20060927

Termination date: 20110711