CN102154422B - Method for extracting functional small peptides from low-value sea fish - Google Patents
Method for extracting functional small peptides from low-value sea fish Download PDFInfo
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Abstract
The invention discloses an industrial production method for extracting functional small peptides from low-value sea fish. The method comprises the following steps of: firstly, pretreating low-value sea fish waste serving as a raw material by using an enzymolysis method; and performing an industrial production process, namely performing enzymolysis by using a composite enzyme, separation, evaporation, concentration and drying, to finally obtain a functional small peptide product, wherein the composite enzyme is prepared from papain and trypsin in a proportion of 1:(0.8-1) according to enzyme activity; and the low-value sea fish waste is enzymolyzed by using an enzymolysis process to obtain the functional small peptide with content of 40.68mg/mL. By means of the method, the utilization rate of protein reaches over 40 percent, the problems of high pH value and difficulty in actual operation in the adoption of alkaline protease single enzyme for hydrolysis are solved, and a flavor effect is also improved.
Description
Technical field
The present invention relates to from animal proteinum to extract the method for little peptide, particularly a kind of from the low value ocean fish method of the little peptide of abstraction function.
Background technology
Biologically active peptides (biological active peptides) refers to have the class functional polypeptide optimizing the organism metabolism environment, be of value to body health.Their the multiple organism metabolism such as Digestive tract, cardiovascular systems, immunity system and neural system systems by acting on body finally play and improve immunity of organisms, reduce the effect that chronic disease occurs.
In recent years, from microorganism or animals and plants, extract separation and have the focus that anti-oxidant, as to improve the different physiological roles such as internal secretion or hormone-like effect biological activity protein small peptide has become current feed nutrition circle research.AB adds the biologically active peptidess such as rich AMP, antibacterial peptide, tethelin and all successfully is applied to Production of Livestock and Poultry.At present, the peptide feedstuff goods of domestic market mostly are imported product, such as DPS (dried porcine saluble), feed large soon, the minority home products are arranged also.DPS is produced in USA, and its main component is chitling mucous membrane proteolysate, except containing abundant amino acid, also contains a large amount of protein oligopeptides, and bioavailability is high, is alleviating stress of baby pigs caused ablaction, reduces diarrhoea, is promoting to have preferably effect aspect the growth.Feeding is the ocean fish protein hydrolysate greatly soon, contains a certain amount of protein small peptide and organic trace element etc., the effect of the breeding performonce fo animals that improves.But because these small peptide goods raw materials are raw material mainly with soybean protein or bathypelagic fish greatly at present, cost is high, has limited its application in feed industry.If can find a kind of albumen small peptide production technique that reasonably has cheaply above-mentioned physiological function concurrently, be used for small peptide production, then this peptide based article will have the great market space.
China is an ocean big country, and fishing resources are very abundant, and output of aquatic products accounts for about 1/3rd of Gross World Product, occupies the No. 1 in the world.But along with polluting and the fishing effort overdevelopment, the ratio of fishing for the low value such as small fish shrimp in the fishery, low quality water product improves constantly, and owing to China's aquatic products processing level is lower, aquatic products processing byproduct output is very big.Therefore, how to improve the utilization ratio of cheap aquatic product, how to develop the market of cheap aquatic product, thereby improve the fishery economic benefit, just become marine industry to be badly in need of a major issue that solves.
The processed and applied of sea low value little fish mainly comprises animal protein feed, gefillte fish, fish oil processing, hydrolyzed fish protein, seasonings and snack food etc.Wherein, because the high speed development of China's aquaculture and special cultivation, the demand of animal protein feed increases very swift and violent, nineteen ninety-five, the amount of finish of China's animal proteinum was 26.1 ten thousand tons, 1996 is 35.9 ten thousand tons, average growth rate per annum surpasses 37%, and however, China every year is still from the fish meal of about 350,000 tons of external imports.The first half of the year in 1999, the fish meal import just reaches 330,000 tons, accounts for 45% of fishery products import total amount.The demand of this explanation China animal protein feed is larger, and the possibility of further expansion will be arranged.In recent years, the development of the fish processing industry of China is rapid, to the also year by year raising of utilization ratio of fishery products raw material.But, annual in prawn and the ocean fish course of processing, produce the feedstuff raw material that a large amount of discarded shrimp heads and low-value small fish be mainly low value and sell, mainly be production feed fish meal, valuable composition is wherein not yet taken full advantage of, economic worth is low.Shrimp head and low-value small fish are the protein raw materials of high-quality, by the biotechnological means little peptide of separation and acquisition nutritional-physiological function therefrom, can be used as novel green trophicity and promote growth additive widespread use aquaculture feed industry, use reducing the animal proteinum raw materials such as fish meal, reduce production costs and to improve food safety significant.In industrial production, from shrimp head and low value ocean fish, extract the little peptide of trophicity function that is rich in fish multiple use is arranged, can be used as nutrition-fortifying agent or foodstuff additive adds in the food, join in the aquatic feeds as novel aquatic products growth promoter, its increment is existing as 20～40 times of feedstuff raw materials, has avoided simultaneously surrounding enviroment are polluted.
The enzyme of using in the hydrolysis of low value ocean fish, great majority are hydrolyzed to free amino acid to the fish body protein only, only have a few enzymes such as papoid, Sumizyme MP (Alcalase) to be hydrolyzed and obtain more dipeptides, tripeptides, and this wherein, Sumizyme MP can produce the less hydrolyzate of bitter taste, but it needs just can effectively play a role under the stronger alkaline condition of pH9, is unfavorable for production application; Obtain the yield of little peptide with papain hydrolysis far above other several enzymes, but relatively poor with its hydrolyzate local flavor that obtains separately, need further to improve.At present, not yet find a kind of gratifying in the low value ocean fish technology of the little peptide of abstraction function, greatly hindered the development and use of low value ocean fish potential value.
Summary of the invention
The objective of the invention is: the technical problems such as deficiency that protein utilization rate is low and the little peptide content of physiological function is low when overcoming prior art hydrolysis low value ocean fish albumen, provide a kind of from the low value ocean fish method of the little peptide of abstraction function.
Technical scheme of the present invention is as follows:
The method of the little peptide of abstraction function from the low value ocean fish, the method of the little peptide of abstraction function from the low value ocean fish, to adopt enzyme solution, at first low value ocean fish tankage raw material is carried out pre-treatment, then add complex enzyme zymohydrolysis, separation, evaporation concentration and dry industrialized producing technology, obtain at last the little peptide product of function.Its technological process is:
(1) raw materials pretreatment: low value ocean fish tankage are thawed, clean, rub.
(2) preparation of enzyme liquid: papoid and trypsinase are mixed, stirring and evenly mixing, it is for subsequent use to get prozyme.
(3) feed intake: be 1: 0.5～1.5 solid-to-liquid ratios with ocean fish tankage and water parts by weight, first water added in the hydrolytic decomposition pot, open moving stirrer, add prozyme 300～700U by every weight in grams ocean fish tankage again, stirring and evenly mixing adds 1000 parts of ocean fish tankage at last, continues stirring and evenly mixing.
(4) enzymolysis: will treat that enzymolysis solution heats, and carry out enzymolysis and the enzyme that goes out is processed by the technique of enzyme solution, and obtain enzymolysis solution.
(5) separate: the hydrolyzed solution behind the enzyme that will go out filters through the filter screen filter of 2mm sieve aperture, removes slag, and filtered liquid is for subsequent use.
(6) concentrated: as will to filter hydrolyzed solution, and pump in the falling-film evaporator, and adopt the mode of following current flow process to carry out evaporation concentration.
(7) drying: the hydrolyzed solution after will concentrating carries out spraying drying in vacuum drier, make meal, obtains the finished product proteolysate.
Above-described prozyme is that papoid and trypsinase are mixed with total prozyme in the ratio of enzyme activity 1: 0.8～1.
Above-described enzyme solution is that hydrolysis temperature is 55 ℃, and soaking time is 4～8h, and enzyme-removal temperature is 85～95 ℃, and the time is 15min～1h.
Above-described evaporation concentration, the control feeding temperature is 60～70 ℃, and vaporization temperature is 65 ℃, and vapor pressure is 0.6～0.65MPa, and feed liquid is 0.4～0.7m/s at velocity in pipes.
Above-described drying, the control temperature is 30～35 ℃, hot blast wind speed 8～10m/s, pressure is less than 0.08kPa.
Advantage of the present invention and positively effect:
1, the used raw material of the little peptide of abstraction function of the present invention is the low value ocean fish, and reaching turns waste into wealth, and increases the benefit, and reduces the effect of cost.
2, the little peptide of abstraction function of the present invention adopts papoid and trypsinase to be mixed with prozyme in 1: 0.8～1 ratio, low value ocean fish albumen is hydrolyzed, can avoid adopting the Sumizyme MP single enzymolysis to exist PH high, actual difficult operational issue has also improved the local flavor effect.
3, the little peptide of abstraction function of the present invention separates the filter screen device filtration of adopting the 2mm sieve aperture, and separating effect is efficient, the filtrate clarification.
4, the present invention extracts and to obtain the little peptide content of function and reach 40.68mg/mL, and protein utilization reaches more than 40%.
Description of drawings:
Fig. 1 is process flow diagram of the present invention.
Embodiment
For ease of understanding the present invention, be elaborated with specific embodiment by reference to the accompanying drawings, but should do not regard each embodiment as any limitation of the invention.
Embodiment
The ocean fish tankage are thawed, clean, drop into mincer and rub, its protein content is surveyed in for subsequent use and sampling.By tankage: the parts by weight of water=1: 0.5～1.5 solid-to-liquid ratios are got the raw materials ready, first water is added in the hydrolytic decomposition pot, open moving stirrer, add papoid and tryptic prozyme 300～700U by every weight in grams ocean fish tankage again, stirring and evenly mixing, add at last 1000 parts of ocean fish tankage, continue stirring and evenly mixing.Then the controlled enzymatic hydrolysis Temperature Setting is at 55 ℃, and soaking time is 4～8h, and the temperature with enzymatic vessel is elevated to 85～95 ℃ at last, and lasting 15min is to the enzymolysis solution enzyme termination reaction of going out.The hydrolyzed solution that will go out behind the enzyme filters through the filter screen filter of 2mm sieve aperture, removes slag, degrease, stays filtered liquid.Hydrolyzed solution after will filtering through the filter screen filter of 2mm sieve aperture pumps in the falling-film evaporator, and the control feeding temperature is 60～70 ℃, vaporization temperature is 65 ℃, vapor pressure is 0.6～0.65MPa, and feed liquid is 0.4～0.7m/s at velocity in pipes, adopts the mode of following current flow process to carry out evaporation concentration.Hydrolyzed solution after concentrated is controlled temperature in vacuum drier be 30～35 ℃, hot blast wind speed 8～10m/s, and pressure is not higher than 0.08kPa, carries out drying, makes meal, obtains the finished product proteolysate.Implementation 7 routine different enzymolysis application conditions and the results are shown in Table 1.
Table 1: different enzymolysis application conditions and table as a result
By the result as can be known, best enzymolysis application art condition is: with the enzyme amount be 500U/g (tankage), enzymolysis 6h, solid-to-liquid ratio be 1: 1, when temperature is 55 ℃, can obtain maximum small peptides is 40.68mg/mL, this moment protein utilization be 40.8%.
Claims (5)
1. the method for the little peptide of abstraction function from the low value ocean fish, it is characterized in that: the method for the little peptide of abstraction function from the low value ocean fish, to adopt enzyme solution, at first low value ocean fish tankage raw material is carried out pre-treatment, then add complex enzyme zymohydrolysis, separation, evaporation concentration and dry industrialized producing technology, obtain at last the little peptide product of function, its technological process is:
(1) raw materials pretreatment: low value ocean fish tankage are thawed, clean, rub;
(2) preparation of enzyme liquid: papoid and trypsinase are mixed, stirring and evenly mixing, it is for subsequent use to get prozyme;
(3) feed intake: be 1:0.5～1.5 solid-to-liquid ratios with ocean fish tankage and water parts by weight, first water added in the hydrolytic decomposition pot, open moving stirrer, add prozyme 300～700U by every weight in grams ocean fish tankage again, stirring and evenly mixing adds 1000 parts of ocean fish tankage at last, continues stirring and evenly mixing;
(4) enzymolysis: will treat that enzymolysis solution heats, and carry out enzymolysis and the enzyme that goes out is processed by the technique of enzyme solution, and obtain enzymolysis solution;
(5) separate: the hydrolyzed solution behind the enzyme that will go out filters through the filter screen filter of 2mm sieve aperture, removes slag, and filtered liquid is for subsequent use;
(6) concentrated: as will to filter hydrolyzed solution, and pump in the falling-film evaporator, and adopt the mode of following current flow process to carry out evaporation concentration;
(7) drying: the hydrolyzed solution after will concentrating carries out spraying drying in vacuum drier, make meal, obtains the finished product proteolysate.
According to claim 1 from the low value ocean fish method of the little peptide of abstraction function, it is characterized in that: prozyme is that papoid and trypsinase are mixed with prozyme in the ratio of enzyme activity 1:0.8～1.
According to claim 1 from the low value ocean fish method of the little peptide of abstraction function, it is characterized in that: enzyme solution is that hydrolysis temperature is 55 ℃, and soaking time is 4～8h, and enzyme-removal temperature is 85～95 ℃, and the time is 15min～1h.
According to claim 1 from the low value ocean fish method of the little peptide of abstraction function, it is characterized in that: evaporation concentration is that the control feeding temperature is 60～70 ℃, vaporization temperature is 65 ℃, and vapor pressure is 0.6～0.65MPa, and feed liquid is 0.4～0.7m/s at velocity in pipes.
According to claim 1 from the low value ocean fish method of the little peptide of abstraction function, it is characterized in that: drying is that the control temperature is 30～35 ℃, hot blast wind speed 8～10m/s, pressure is less than 0.08kPa.
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| CN102628073B (en) * | 2012-03-28 | 2013-11-20 | 东北农业大学 | Glycosylated lactalbumin polypeptide with antioxidant activity as well as preparation method and application of glycosylated lactalbumin polypeptide |
| CN103088097A (en) * | 2013-01-04 | 2013-05-08 | 中国科学院海洋研究所 | Preparation method of matreel active peptide |
| CN104273313A (en) * | 2014-09-12 | 2015-01-14 | 浙江海洋学院 | Method for reasonably utilizing leftovers of Spanish mackerel and common Japanese mackerel |
| CN107361242A (en) * | 2017-07-20 | 2017-11-21 | 通化师范学院 | A kind of feed addictive for improving cold water meat quality of fish and application thereof |
| CN107385000A (en) * | 2017-07-31 | 2017-11-24 | 兰溪市哥特生物技术有限公司 | The method of abstraction function small peptide from deep-sea fish |
| CN107736610B (en) * | 2017-09-12 | 2021-04-20 | 山东圣琪生物有限公司 | Yeast extract rich in small peptide and preparation method thereof |
| CN108065043A (en) * | 2017-12-20 | 2018-05-25 | 广东博沃特生物科技有限公司 | A kind of small-molecular peptides fish meal and its preparation method and application |
| CN109998082A (en) * | 2019-05-17 | 2019-07-12 | 福建师范大学闽南科技学院 | Seafood seasoning powder composition and preparation method thereof |
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