CN108065043A - A kind of small-molecular peptides fish meal and its preparation method and application - Google Patents
A kind of small-molecular peptides fish meal and its preparation method and application Download PDFInfo
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- CN108065043A CN108065043A CN201711383576.0A CN201711383576A CN108065043A CN 108065043 A CN108065043 A CN 108065043A CN 201711383576 A CN201711383576 A CN 201711383576A CN 108065043 A CN108065043 A CN 108065043A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
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Abstract
The invention discloses a kind of small-molecular peptides fish meal and its preparation method and application.The preparation method of small-molecular peptides fish meal of the present invention includes the following steps:Fresh discarded ocean fish leftover bits and pieces with clear water is rinsed, is scaled and internal organ, then rubs into minced fillet, it adds in water and complex enzyme is digested, obtain enzymolysis solution, then enzymolysis solution is filtered, gained filtrate obtains small-molecular peptides fish meal through enzyme deactivation sterilizing, high-pressure spray-drying;Wherein, ocean fish leftover bits and pieces includes processing byproduct and low value fishes and shrimps;The ratio between the quality of minced fillet and the volume of water are 1:1~5;Complex enzyme includes alkali protease and neutral proteinase, and addition is the 0.1~3% of quality of fish filling;The condition of enzymolysis is:PH6~9,45~60 DEG C of temperature, 40~240min of enzymolysis time.The small-molecular peptides content of small-molecular peptides fish meal of the present invention is up to 63.08%, free aminoacid content accounts for total amino acid content more than 60% up to more than 19.73%, wherein essential amino acids content, and TVB-N content is less than 100mg/100g, freshness is good, has a good application prospect.
Description
Technical field
The invention belongs to aquaculture technical field, it is more particularly related to a kind of using enzyme solution production
Small-molecular peptides fish meal and its application.
Background technology
The discarded ocean fish that fishery fishing and food processing generate, it is resourceful.But aquatic products deep processing at present compared with
It is few, the 30.7% of aquatic products total amount is only accounted for, major part has only carried out low value utilization, such as produces traditional fish meal as raw material,
There is production technology is unreasonable, quality it is universal it is relatively low, palatability is poor, nutritive value is not high low with digestive utilization ratio etc. asks
Topic.Meanwhile the mode that this low value utilizes easily causes the wasting of resources and environmental pollution.
It digests in fish meal and shows the mixture of small peptide and amino acid containing a large amount of free amino acids and small-molecular peptides, research
Absorptivity and infiltration rate will be better than amino acid, and small peptide can protected amino acid be not destroyed, synthesized again in organism
During new protein, the mixture of small peptide and amino acid can save the process that a part reconfigures, and have higher biology
Potency.But the current existing enzymolysis related patents of fish meal and research, enzymolysis process relative coarseness, cause most related productions
Product free aminoacid content and small-molecular peptides content are relatively low, and proportion is respectively smaller than 15% and 40%, and not to enzymolysis
The freshness of fish meal carries out stringent control, and TVB-N content in product is caused to be higher than 120mg/100g.
The content of the invention
It is an object of the invention to:Overcome above-mentioned deficiency in the prior art, provide a kind of small-molecular peptides fish meal and
Its preparation method and application.
In order to realize foregoing invention purpose, the present invention provides a kind of preparation method of small-molecular peptides fish meal, including such as
Lower step:
Fresh discarded ocean fish leftover bits and pieces with clear water is rinsed, is scaled and internal organ, then rubs into minced fillet, adds in water and multiple
Synthase is digested, and obtains enzymolysis solution, then enzymolysis solution is filtered, and gained filtrate obtains through enzyme deactivation sterilizing, high-pressure spray-drying
To small-molecular peptides fish meal;
Wherein, the ocean fish leftover bits and pieces includes processing byproduct and low value fishes and shrimps;
The ratio between the quality of the minced fillet and the volume of water are 1:1~5
The complex enzyme includes alkali protease and neutral proteinase, and addition is the 0.1~3% of quality of fish filling, described
Alkali protease is derived from bacillus pumilus (Bacillus pumilus), and the neutral proteinase is derived from bacillus subtilis
Bacterium (Bacillus subtilis);It is that market is commercially available.
The condition of the enzymolysis is:PH6~9,45~60 DEG C of temperature, 40~240min of enzymolysis time.
Preferably, the addition of the upper complex enzyme is the 1.5~2% of quality of fish filling.
Preferably, according to single enzyme enzyme activity gauge, the ratio of the alkali protease and neutral proteinase is 1:1.
Preferably, the condition of the enzymolysis is:PH7.5~8.5,48~58 DEG C of temperature, 180~210min of enzymolysis time.
Preferably, the filtering is the filter cloth plate compression using 200~400 mesh.
Preferably, the enzyme deactivation sterilizing is by the way of boiling water bath.
Preferably, the condition of the high-pressure spray-drying is:140~180 DEG C of spray drying tower inlet temperature, outlet temperature
65~85 DEG C, 12000 revs/min of rotary head speed.
The present invention also provides a kind of small-molecular peptides fish meal, are prepared by above-mentioned preparation method.
Small-molecular peptides fish meal of the present invention can be used as feed to be used for livestock-raising.
Compared with the prior art, the present invention has the advantages that:
(1) present invention selection freshness is good, and no putrid and deteriorated discarded ocean fish is raw material, once purged, rubs into gruel,
The contact area of enzyme and storeroom in enzymolysis process can be increased;Meanwhile can be kept stirring in enzymolysis process, to improve enzymolysis effect
Rate makes material fully digest.
(2) present invention can be ensured product freshness, be avoided corruption using low temperature rapid enzymolysis.
(3) present invention can carry out secondary enzymolysis by the way of complex enzyme hydrolysis to material, improve small peptide and free amine group
Acid content.
(4) present invention can remove the fish scale fish not digested completely by the way of 200~400 mesh filter cloth plate compressions
Bone, purified peptide.
(5) present invention is sterilized by boiling water bath enzyme deactivation, can be effective on the premise of ensureing that nutritional ingredient is not destroyed
Excessively enzymolysis and living contaminants are avoided, reduces products obtained therefrom TVB-N content.
(6) through measuring:Small-molecular peptides fish meal is made using method provided by the present invention, small peptide content is reachable
63.08%, free aminoacid content up to more than 19.73%, wherein essential amino acids content account for total amino acid content 60% with
On, TVB-N content is less than 100mg/100g.Protein be in animal body in a manner of small peptide combination amino acid into
Row absorbs, and small peptide and free aminoacid content height can effectively improve absorption rate;And essential amino acid is animal
It must be absorbed from food and essential amino acid, to promoting to grow, carry out eubolism, sustaining life provides substance
Basis is of great significance;Volatile Base Nitrogen is with living contaminants small peptide and free amino acid to be caused to turn due to excessively digesting
Change, be the important indicator for influencing freshness, to feed safety, animal health cultivation has a major impact with safety in production, contains
Measure that low proof freshness is good, and it is poor that content Gao Ze represents freshness, nutrition destroy it is serious, easily led to during feeding livestock and poultry diarrhea,
Indigestion or even food poisoning.
(7) operation is simple and feasible for the method for the present invention, and large-scale production is at low cost, the environmental pollution by the way of enzymolysis
Small, the purity of gained small-molecular peptides fish meal is high, and freshness is good, greatly improves the added value of discarded ocean fish, has significant
Economic interests and far-reaching social benefit.
Specific embodiment
In order to become apparent from the purpose of the present invention, technical solution and advantageous effects, with reference to embodiments, to this
Invention is further elaborated.It should be appreciated that the embodiment described in this specification is just for the sake of this hair of explanation
It is bright, be not intended to limit the present invention, parameter, ratio of embodiment etc. can adaptation to local conditions make a choice and substance had no to result
It influences.
Embodiment 1
A kind of preparation method of small-molecular peptides fish meal, this method comprise the following steps:
S1. the discarded ocean fish of fresh fishing is selected, clear water rinses;
S2. the full fish of ocean fish after cleaning is rubbed into gruel;
S3. it will rub into the raw material after gruel and be put into enzymatic vessel, minced fillet is 1 with water ratio:1(m:V), pH to 8.5, temperature are adjusted
Degree adds in compound enzyme powder (alkali protease to 53 DEG C, by quality of fish filling percentage for 1.5%:Neutral proteinase is 1:1), digest
Time is 200min;
S4. 200 mesh filter cloth plate compression of solution is digested, collects filtered fluid;
S5. enzymolysis solution boiling water bath (95 DEG C) enzyme deactivation sterilizing after filtering;
S6. 155 DEG C of spray drying tower inlet temperature, 80 DEG C of outlet temperature, 12000 revs/min of rotary head speed obtain small point
Sub- peptide fish meal.
After measured, in gained small-molecular peptides fish meal, moisture 3.0%, crude protein content 90.5%, crude fat content
2.0%, Small Peptides content is up to 63.08%;Free aminoacid content is up to more than 19.73%, and amino acid classes are complete,
Wherein essential amino acids content accounts for total amino acid content more than 60.01%;TVB-N content is less than 100mg/100g.Trip
Isolated amino acid forms and each component content, is shown in Table 1.Small-molecular peptides fish meal different proportion obtained by use substitutes traditional fish meal feeding and educates
Fertile phase pig, obtains good feeding effect, significantly improves feed intake, the daily gain of growing and fattening pigs, reduces feedstuff-meat ratio, save raising into
This, there is good economic benefit.
Table 1
Embodiment 2
A kind of preparation method of small-molecular peptides fish meal, this method comprise the following steps:
S1. the discarded ocean fish of fresh fishing is selected, clear water rinses;
S2. the ocean fish after cleaning is scaled, internal organ is gone to rub into gruel;
S3. it will rub into the raw material after gruel and be put into enzymatic vessel, minced fillet is 1 with water ratio:4(m:V), pH to 7.5, temperature are adjusted
Degree adds in compound enzyme powder (alkali protease to 48 DEG C, by quality of fish filling percentage for 2%:Neutral proteinase is 1:1), during enzymolysis
Between be 200min;
S4. 300 mesh filter cloth plate compression of solution is digested, collects filtered fluid;
S5. enzymolysis solution boiling water bath (95 DEG C) enzyme deactivation sterilizing after filtering;
S6. 145 DEG C of spray drying tower inlet temperature, 70 DEG C of outlet temperature, 12000 revs/min of rotary head speed obtain small point
Sub- peptide fish meal.
After measured, in gained small-molecular peptides fish meal, moisture 3.5%, crude protein content 89.5%, crude fat content
2.0%, Small Peptides content is up to 62.75%;Free aminoacid content is up to more than 20.06%, and amino acid classes are complete,
Wherein essential amino acids content accounts for total amino acid content more than 59.97%;TVB-N content is only up to 76mg/
100g.Free amino acid forms and each component content, is shown in Table 2.Small-molecular peptides fish meal different proportion is made using this method to substitute
Traditional fish meal feeding cow can effectively improve content of milk protein in the output of milk and cow's milk, improve production performance and dairy products
Matter has good application prospect.
Table 2
Embodiment 3
A kind of preparation method of small-molecular peptides fish meal, this method comprise the following steps:
S1. the discarded ocean fish of fresh fishing is selected, clear water rinses;
S2. the ocean fish after cleaning scaled, boned, internal organ, decaptitating is gone to rub into gruel;
S3. it will rub into the raw material after gruel and be put into enzymatic vessel, minced fillet is 1 with water ratio:2(m:V), pH to 8.0, temperature are adjusted
Degree adds in compound enzyme powder (alkali protease to 58 DEG C, by quality of fish filling percentage for 1.75%:Neutral proteinase is 1:1), enzyme
The solution time is 180min;
S4. 400 mesh filter cloth plate compression of solution is digested, collects filtered fluid;
S5. enzymolysis solution boiling water bath (95 DEG C) enzyme deactivation sterilizing after filtering;
S6. 150 DEG C of spray drying tower inlet temperature, 75 DEG C of outlet temperature, 12000 revs/min of rotary head speed, described in acquisition
Small-molecular peptides fish meal.
After measured, in gained small-molecular peptides fish meal, moisture 4.0%, crude protein content 86.0%, crude fat content
2.5%, Small Peptides content is up to 61.90%;Free aminoacid content is up to more than 20.91%, and amino acid classes are complete,
Wherein essential amino acids content accounts for total amino acid content more than 59.45%;TVB-N content is only up to 80mg/
100g.Free amino acid forms and each component content, is shown in Table 3.Small-molecular peptides fish meal different proportion is made using this method to substitute
Traditional fish meal makes young age cat after Pet cat feed feeding wean, because of its special seafood fragrance, can effectively improve palatability,
Young age cat phenomenon particular about food is reduced, improves its body immunity.
Table 3
According to the disclosure and teachings of the above specification, those skilled in the art in the invention can also be to above-mentioned embodiment party
Formula carries out appropriate change and modification.Therefore, the invention is not limited in specific embodiment disclosed and described above, to this
Some modifications and changes of invention should also be as falling into the scope of the claims of the present invention.In addition, although this specification
In used some specific terms, but these terms are merely for convenience of description, do not limit the present invention in any way.
Claims (9)
1. a kind of preparation method of small-molecular peptides fish meal, which is characterized in that include the following steps:
Fresh discarded ocean fish leftover bits and pieces with clear water is rinsed, is scaled and internal organ, then rubs into minced fillet, adds in water and complex enzyme
It is digested, obtains enzymolysis solution, then enzymolysis solution is filtered, gained filtrate obtains small through enzyme deactivation sterilizing, high-pressure spray-drying
Molecular peptide fish meal;
Wherein, the ocean fish leftover bits and pieces includes processing byproduct and low value fishes and shrimps;
The ratio between the quality of the minced fillet and the volume of water are 1:1~5;
The complex enzyme includes alkali protease and neutral proteinase, and addition is the 0.1~3% of quality of fish filling, the alkalescence
Protease is derived from bacillus pumilus (Bacillus pumilus), and the neutral proteinase is derived from bacillus subtilis
(Bacillus subtilis);
The condition of the enzymolysis is:PH6~9,45~60 DEG C of temperature, 40~240min of enzymolysis time.
2. the preparation method of small-molecular peptides fish meal according to claim 1, which is characterized in that the addition of the complex enzyme
For the 1.5~2% of quality of fish filling.
3. the preparation method of small-molecular peptides fish meal according to claim 1, which is characterized in that according to single enzyme enzyme activity gauge,
The ratio of the alkali protease and neutral proteinase is 1:1.
4. the preparation method of small-molecular peptides fish meal according to claim 1, which is characterized in that the condition of the enzymolysis is:
PH7.5~8.5,48~58 DEG C of temperature, 180~210min of enzymolysis time.
5. the preparation method of small-molecular peptides fish meal according to claim 1, which is characterized in that the filtering is to use 200
The filter cloth plate compression of~400 mesh.
6. the preparation method of small-molecular peptides fish meal according to claim 1, which is characterized in that the enzyme deactivation sterilizing is to use
The mode of boiling water bath.
7. the preparation method of small-molecular peptides fish meal according to claim 1, which is characterized in that the high-pressure spray-drying
Condition is:140~180 DEG C of spray drying tower inlet temperature, 65~85 DEG C of outlet temperature, 12000 revs/min of rotary head speed.
Small point that 8. the preparation method of small-molecular peptides fish meal described in any one claim is prepared in claim 1~7
Sub- peptide fish meal.
9. small-molecular peptides fish meal described in claim 8 is as feed, the application in livestock-raising.
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Cited By (2)
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CN111671016A (en) * | 2020-06-24 | 2020-09-18 | 福建天马科技集团股份有限公司 | High-efficiency fish meal for aquatic compound feed and preparation method thereof |
WO2022043745A1 (en) * | 2020-08-28 | 2022-03-03 | Kerry Luxembourg S.a.r.l. | Feed composition supplemented with a protease combination comprising neutral metalloprotease and serine alkaline protease at a given ratio |
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Cited By (2)
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