CN107557419A - A kind of preparation method of low value fish-bone polypeptide chelate calcium - Google Patents
A kind of preparation method of low value fish-bone polypeptide chelate calcium Download PDFInfo
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Abstract
The invention discloses a kind of preparation method of low value fish-bone polypeptide chelate calcium, including a kind of preparation method of low value fish-bone polypeptide chelate calcium, including rough fishbone dust, fishbone dust fermentation, stepwise discretization, isoelectric precipitation, chelating and freeze-drying.Have the beneficial effect that:Small molecule bone polypeptide is prepared using the method digested using marine protein enzyme and land proteinase synergy.The bone polypeptide molecular weight being prepared is small, the bone polypeptide chelate calcium obtained after being chelated with calcium ion, and the absorptivity in human body is high, and does not have the generation of blood calcium symptom.Human body is good to bone polypeptide chelate calcium calcium absorption effect, and long-term use of calcium apparent absorptivity, calcium Retention, bone density, bone mineral salt content, cortex of bone area, femur energy absorption etc. are in significantly increase trend.
Description
Technical field
The present invention relates to health products preparation field, specifically a kind of preparation method of low value fish-bone polypeptide chelate calcium.
Background technology
China is aquatic products big producer, and the yield of annual China fresh-water fishes and ocean fish is in the trend that grows steadily.In recent years,
China's fish processing industry is quickly grown, and existing oneself forms the classes such as refrigerated products, salted fish product, feeding fish meal, minced fillet and its product
The product of type.Surimi product has the characteristics that high protein, low fat, numerous in variety, storage endurance are liked by consumers in general deeply because of it
Love, turn into the Main Means of fish processing, but its flesh collecting rate typically only 25 ~ 30%.Adopt and contain what is enriched in the remaining fish body of meat
The mineral matters such as calcium, phosphorus, it is a kind of good natural calcium source.
Naturally occurring Marine Bioactive Peptides due in organism content it is low, and extract difficult, it is difficult to realize a large amount of
Needed for production supply, chemical artificial synthetic polypeptide is costly.So sight is more invested exploitation proteolysis production by people
Come in this approach of thing acquisition active peptides.Because the environment and terrestrial life of marine organisms existence are entirely different, such as high pressure, low
The extreme environment such as temperature, high temperature and high salt, in order to adapt to these extreme marine biological environments, marine protein matter no matter amino
The composition or the sequence of amino acid of acid are all very different with terrestrial life albumen, meanwhile, marine protein resource without
By being all far longer than land protein resource in type and quantity, and do not developed well.Miscellaneous ocean egg
In casamino acid sequence, potential many amino acid sequences with bioactivity, with special protease hydrolytic, just discharge
Active peptide fragment.Marine protein resource is the important of the important albumen group food of the 21 century mankind and bioactive substance
Source.The current marine protein total resources in China comes out at the top in countries in the world, but the protide aquatic products that China is current
In product, in addition to a part is directly edible, major part is made food by simple process technology and enters market, and process technology falls
Afterwards, product structure is single, and value-added content of product is low so that the competitiveness of product in the international market is poor.Therefore, China is badly in need of
Marine protein resource is optimized and utilized and high-valued processing.
The content of the invention
It is an object of the invention to provide a kind of preparation method of low value fish-bone polypeptide chelate calcium, human body is to being prepared
The calcium absorption effect of low value fish-bone polypeptide is good, and long-term use of calcium apparent absorptivity, calcium Retention, bone density, bone mineral salt contain
Amount, cortex of bone area, femur energy absorption etc. are in significantly increase trend.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is:A kind of low value fish-bone polypeptide chelate
The preparation method of calcium, including rough fishbone dust, fishbone dust fermentation, stepwise discretization, isoelectric precipitation, chelating and freeze-drying.Fish
Bone meal fermentation step is:Fishbone dust is mixed to prepare lactobacillus-fermented culture medium, high temperature with pure water, glucose and potato extract solution
Sterilizing, inoculating lactic acid bacterium after cooling, ferment 3 ~ 7d under the conditions of 28 ~ 32 DEG C, is concentrated and dried grinding and obtains fishbone dust of fermenting.Fish-bone
Powder is 14 ~ 25 with pure water, glucose and potato extract solution mass ratio:60~75:2~4:3~6;Lactic acid bacteria is plant embryo breast bar
Bacterium.The organic acids and free amino acid, polysaccharide, enzyme etc. such as lactic acid, acetic acid, pyruvic acid, butanedioic acid, fumaric acid are produced in fermentation process
Metabolin, the calcium in fish-bone is converted into free state from reference state.The calcium ion content that dissociates in zymotic fluid is far above milk, and
It is also high containing the needed by human body elements such as phosphorus, magnesium, iron, zinc, nutritive value.
Rough fishbone dust step is:30 ~ 40wt% sodium carbonate liquors are added in the fish-bone of grinding, soak 25 ~ 35min, fish
The volume ratio of bone and sodium carbonate liquor is 1:2.5 ~ 4.5, filtering, precipitation is cleaned with pure water, is re-fed into pressure vessel, control pressure
0.14 ~ 0.18MPa of power, 4 ~ 7min of boiling, drying and crushing obtain fishbone dust.First use undaria pinnitafida protease, on this basis after
It is continuous to be digested using flavor protease, it can obtain bone polypeptide.It is above-mentioned to be digested using using marine protein enzyme and land proteinase synergy
Method prepare small molecule bone polypeptide, have significant creative.The bone polypeptide molecular weight being prepared is small, is chelated with calcium ion
The bone polypeptide chelate calcium obtained afterwards, the absorptivity in human body is high, and does not have the generation of blood calcium symptom.
Stepwise discretization step is that 20 ~ 30 times of volume pure water homogenate are added in fishbone dust, adds the undaria pinnitafida eggs of 5 ~ 8wt ‰
White enzyme and the bioactive peptides of 0.2 ~ 0.7wt ‰, in 25 ~ 35 DEG C, digesting 30 ~ 40min under the conditions of pH7.0 ~ 9.0, regulation pH is 6.0 ~
7.0,1 ~ 3wt ﹪ flavor proteases are added, 15 ~ 25min is digested in 52 ~ 60 DEG C, obtains enzymolysis liquid;The amino acid sequence of bioactive peptide
It is classified as SCASRCKVSFRCAVSFCICKCFRC.Above-mentioned bioactive peptide has certain activation to undaria pinnitafida protease, the most
Importantly, above-mentioned bioactive peptide and calcium ion be by electrostatic reaction forming, and by calcium ion parcel wherein, make calcium ion with
The contact area for digesting obtained bone polypeptide is greatly decreased, and chelatropic reaction hardly occurs.
For isoelectric precipitation step for enzymolysis liquid is filtered, the pH value for adjusting filtrate is 4.5 ~ 4.8, and centrifuging and taking is sunk after standing
Form sediment.Under above-mentioned pH environment, bone polypeptide is in electroneutral, is slightly soluble in water, most of to be separated with precipitation form from the aqueous solution.
Chelation step is by 24 ~ 48h of nanofiltration in centrifuged supernatant and pure water, takes the pure water after nanofiltration, concentrates, the electricity such as addition
The fish-bone polypeptide that point precipitation obtains, in 50 ~ 55 DEG C, pH chelates 1.5 ~ 2h under the conditions of being 6.8 ~ 7.2, adds 2 ~ 4 times of absolute ethyl alcohols,
2 ~ 4h, centrifuging and taking precipitation are stood at 25 ~ 35 DEG C.In pure water after nanofiltration containing abundant vitamin, a large amount of calcium ions and phosphorus,
The needed by human body element such as magnesium, iron, zinc, bone polypeptide chelate calcium is obtained after chelating, human body is good to its calcium absorption effect, long-term use of
Calcium apparent absorptivity, calcium Retention, bone density, bone mineral salt content, cortex of bone area, femur energy absorption etc. increase in obvious
Main trend.
It is freeze-dried addition 20 ~ 35wt% polyethylene Topiramate Los oxazolidinone in step and obtains low as lyophilisation additives, freeze-drying
It is worth fish-bone polypeptide chelate calcium powder.Bone polypeptide chelate calcium needs to be maintained at property during storage containing suitable residual moisture
Stable, the hydrogen bond for making protein molecule surface and polar group exposure are denatured by excessive drying.In freezing dry process, add
Enter polyethylene Topiramate Los oxazolidinone, the albumen of bone polypeptide chelate calcium can be protected to stretch, quaternary structure, so as to bone polypeptide chelate calcium activity
Protected.
Compared with prior art, the advantage of the invention is that:
1. first using undaria pinnitafida protease, continue to digest using flavor protease on this basis, can obtain bone polypeptide.It is above-mentioned
Small molecule bone polypeptide is prepared using the method digested using marine protein enzyme and land proteinase synergy, there is significant create
Property.The bone polypeptide molecular weight being prepared is small, the bone polypeptide chelate calcium obtained after being chelated with calcium ion, the absorptivity in human body
Height, and the generation of blood calcium symptom is not had.Human body is good to bone polypeptide chelate calcium calcium absorption effect, long-term use of calcium apparent absorption
Rate, calcium Retention, bone density, bone mineral salt content, cortex of bone area, femur energy absorption etc. are in significantly increase trend.
2. amino acid sequence is added when undaria pinnitafida protease hydrolyzed deep-sea fish is homogenized is
SCASRCKVSFRCAVSFCICKCFRC bioactive peptide, above-mentioned bioactive peptide have certain activation to make to undaria pinnitafida protease
With most importantly, above-mentioned bioactive peptide wraps up wherein with calcium ion by electrostatic reaction forming, and by calcium ion, makes
Calcium ion is greatly decreased with digesting the contact area of obtained bone polypeptide, and chelatropic reaction hardly occurs.
Embodiment
The present invention program is described further below by embodiment:
Embodiment 1:
A kind of preparation method of low value fish-bone polypeptide chelate calcium, including rough fishbone dust, fishbone dust fermentation, stepwise discretization, etc. electricity
Point precipitation, chelating and freeze-drying.Fishbone dust fermentation step is:Fishbone dust mixes with pure water, glucose and potato extract solution
Lactobacillus-fermented culture medium is made, high-temperature sterilization, inoculating lactic acid bacterium after cooling, ferment 3 ~ 7d under the conditions of 28 ~ 32 DEG C, and concentration is dry
Dry grinding obtains fishbone dust of fermenting.Fishbone dust is 14 ~ 25 with pure water, glucose and potato extract solution mass ratio:60~75:2~
4:3~6;Lactic acid bacteria is plant embryo lactobacillus.Lactic acid, acetic acid, pyruvic acid, butanedioic acid, fumaric acid etc. are produced in fermentation process to be had
The metabolin such as machine acid and free amino acid, polysaccharide, enzyme, free state is converted into by the calcium in fish-bone from reference state.Zymotic fluid middle reaches
It is far above milk from calcium ion content, and it is also high containing the needed by human body elements such as phosphorus, magnesium, iron, zinc, nutritive value.
Rough fishbone dust step is:30 ~ 40wt% sodium carbonate liquors are added in the fish-bone of grinding, soak 25 ~ 35min, fish
The volume ratio of bone and sodium carbonate liquor is 1:2.5 ~ 4.5, filtering, precipitation is cleaned with pure water, is re-fed into pressure vessel, control pressure
0.14 ~ 0.18MPa of power, 4 ~ 7min of boiling, drying and crushing obtain fishbone dust.First use undaria pinnitafida protease, on this basis after
It is continuous to be digested using flavor protease, it can obtain bone polypeptide.It is above-mentioned to be digested using using marine protein enzyme and land proteinase synergy
Method prepare small molecule bone polypeptide, have significant creative.The bone polypeptide molecular weight being prepared is small, is chelated with calcium ion
The bone polypeptide chelate calcium obtained afterwards, the absorptivity in human body is high, and does not have the generation of blood calcium symptom.
Stepwise discretization step is that 20 ~ 30 times of volume pure water homogenate are added in fishbone dust, adds the undaria pinnitafida eggs of 5 ~ 8wt ‰
White enzyme and the bioactive peptides of 0.2 ~ 0.7wt ‰, in 25 ~ 35 DEG C, digesting 30 ~ 40min under the conditions of pH7.0 ~ 9.0, regulation pH is 6.0 ~
7.0,1 ~ 3wt ﹪ flavor proteases are added, 15 ~ 25min is digested in 52 ~ 60 DEG C, obtains enzymolysis liquid;The amino acid sequence of bioactive peptide
It is classified as SCASRCKVSFRCAVSFCICKCFRC.Above-mentioned bioactive peptide has certain activation to undaria pinnitafida protease, the most
Importantly, above-mentioned bioactive peptide and calcium ion be by electrostatic reaction forming, and by calcium ion parcel wherein, make calcium ion with
The contact area for digesting obtained bone polypeptide is greatly decreased, and chelatropic reaction hardly occurs.
For isoelectric precipitation step for enzymolysis liquid is filtered, the pH value for adjusting filtrate is 4.5 ~ 4.8, and centrifuging and taking is sunk after standing
Form sediment.Under above-mentioned pH environment, bone polypeptide is in electroneutral, is slightly soluble in water, most of to be separated with precipitation form from the aqueous solution.
Chelation step is by 24 ~ 48h of nanofiltration in centrifuged supernatant and pure water, takes the pure water after nanofiltration, concentrates, the electricity such as addition
The fish-bone polypeptide that point precipitation obtains, in 50 ~ 55 DEG C, pH chelates 1.5 ~ 2h under the conditions of being 6.8 ~ 7.2, adds 2 ~ 4 times of absolute ethyl alcohols,
2 ~ 4h, centrifuging and taking precipitation are stood at 25 ~ 35 DEG C.In pure water after nanofiltration containing abundant vitamin, a large amount of calcium ions and phosphorus,
The needed by human body element such as magnesium, iron, zinc, bone polypeptide chelate calcium is obtained after chelating, human body is good to its calcium absorption effect, long-term use of
Calcium apparent absorptivity, calcium Retention, bone density, bone mineral salt content, cortex of bone area, femur energy absorption etc. increase in obvious
Main trend.
It is freeze-dried addition 20 ~ 35wt% polyethylene Topiramate Los oxazolidinone in step and obtains low as lyophilisation additives, freeze-drying
It is worth fish-bone polypeptide chelate calcium powder.Bone polypeptide chelate calcium needs to be maintained at property during storage containing suitable residual moisture
Stable, the hydrogen bond for making protein molecule surface and polar group exposure are denatured by excessive drying.In freezing dry process, add
Enter polyethylene Topiramate Los oxazolidinone, the albumen of bone polypeptide chelate calcium can be protected to stretch, quaternary structure, so as to bone polypeptide chelate calcium activity
Protected.
Embodiment 2:
A kind of fishbone dust and the most preferably preparation method of protein peptide mixture chewable tablets, comprise the following steps:
1)Rough fishbone dust:34wt% sodium carbonate liquors are added in the fish-bone of grinding, soak 32min, fish-bone and sodium carbonate liquor
Volume ratio be 1:3.6, filtering, precipitation is cleaned with pure water, is re-fed into pressure vessel, control pressure 0.15MPa, boiling
5min, drying and crushing obtain fishbone dust;
2)Fishbone dust is fermented:Fishbone dust is mixed to prepare lactobacillus-fermented culture medium with pure water, glucose and potato extract solution, high
Temperature sterilizing, inoculating lactic acid bacterium after cooling, ferment 6d under the conditions of 30 DEG C, is concentrated and dried grinding and obtains fishbone dust of fermenting.Fishbone dust
It is 20 with pure water, glucose and potato extract solution mass ratio:65:3: 6;Lactic acid bacteria is plant embryo lactobacillus;
3)Stepwise discretization:The homogenate of 24 times of volume pure water is added in fishbone dust, add the undaria pinnitafida protease of 7wt ‰ and
The bioactive peptides of 0.6wt ‰, in 28 DEG C, 35min being digested under the conditions of pH8.0, regulation pH is 6.6, adds 2wt ﹪ flavor proteases,
19min is digested in 55 DEG C, obtains enzymolysis liquid;The amino acid sequence of bioactive peptide is SCASRCKVSFRCAVSFCICKCFRC;
4)Isoelectric precipitation:Enzymolysis liquid is filtered, the pH value for adjusting filtrate is 4.6, and centrifuging and taking precipitates after standing;
5)Chelating:By nanofiltration 24h in centrifuged supernatant and pure water, the pure water after nanofiltration is taken, is concentrated, isoelectric precipitation is added and obtains
Fish-bone polypeptide, in 53 DEG C, pH chelates 1.8h under the conditions of being 7.0, adds 3 times of absolute ethyl alcohols, 3h, centrifuging and taking are stood at 30 DEG C
Precipitation;
6)Freeze-drying:Isometric water is added in precipitation, adds 32wt% polyethylene Topiramate Los oxazolidinone as lyophilisation additives, it is cold
Jelly is dried to obtain protein peptide powder.
Embodiment 3:
A kind of preparation method of low value fish-bone polypeptide chelate calcium, comprises the following steps:
1)Rough fishbone dust:30wt% sodium carbonate liquors are added in the fish-bone of grinding, soak 30min, fish-bone and sodium carbonate liquor
Volume ratio be 1:3.5, filtering, precipitation is cleaned with pure water, is re-fed into pressure vessel, control pressure 0.13MPa, boiling
3min, drying and crushing obtain fishbone dust;
2)Fishbone dust is fermented:Fishbone dust is mixed to prepare lactobacillus-fermented culture medium with pure water, glucose and potato extract solution, high
Temperature sterilizing, inoculating lactic acid bacterium after cooling, ferment 7d under the conditions of 301 DEG C, is concentrated and dried grinding and obtains fishbone dust of fermenting.Fishbone dust
It is 22 with pure water, glucose and potato extract solution mass ratio:70:3: 6;Lactic acid bacteria is plant embryo lactobacillus;
3)Stepwise discretization:The homogenate of 22 times of volume pure water is added in fishbone dust, add the undaria pinnitafida protease of 7wt ‰ and
The bioactive peptides of 0.6wt ‰, in 28 DEG C, 35min being digested under the conditions of pH8.0, regulation pH is 6.6, adds 2wt ﹪ flavor proteases,
19min is digested in 55 DEG C, obtains enzymolysis liquid;The amino acid sequence of bioactive peptide is SCASRCKVSFRCAVSFCICKCFRC;
4)Isoelectric precipitation:Enzymolysis liquid is filtered, the pH value for adjusting filtrate is 4.6, and centrifuging and taking precipitates after standing;
5)Chelating:By nanofiltration 24h in centrifuged supernatant and pure water, the pure water after nanofiltration is taken, is concentrated, isoelectric precipitation is added and obtains
Fish-bone polypeptide, in 53 DEG C, pH chelates 1.8h under the conditions of being 7.0, adds 3 times of absolute ethyl alcohols, 3h, centrifuging and taking are stood at 30 DEG C
Precipitation;
6)Freeze-drying:Isometric water is added in precipitation, adds 32wt% polyethylene Topiramate Los oxazolidinone as lyophilisation additives, it is cold
Jelly is dried to obtain protein peptide powder.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Pujiang County is safe such as food science and technology Co., Ltd
<120>A kind of preparation method of low value fish-bone polypeptide chelate calcium
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 24
<212> PRT
<213>It is artificial synthesized
<400> 1
Ser Cys Ala Ser Arg Cys Lys Val Ser Phe Arg Cys Ala Val Ser Phe
1 5 10 15
Cys Ile Cys Lys Cys Phe Arg Cys
20
Claims (7)
1. a kind of preparation method of low value fish-bone polypeptide chelate calcium, including rough fishbone dust, fishbone dust fermentation, stepwise discretization, etc.
Electricity point precipitation, chelating and freeze-drying, it is characterised in that:Described fishbone dust fermentation step is:Fishbone dust and pure water, glucose
Lactobacillus-fermented culture medium, high-temperature sterilization, inoculating lactic acid bacterium after cooling, in 28 ~ 32 DEG C of bars are mixed to prepare with potato extract solution
Ferment 3 ~ 7d under part, is concentrated and dried grinding and obtains fishbone dust of fermenting.
A kind of 2. preparation method of low value fish-bone polypeptide chelate calcium according to claim 1, it is characterised in that:Described fish
Bone meal is 14 ~ 25 with pure water, glucose and potato extract solution mass ratio:60~75:2~4:3~6;The lactic acid bacteria is plant embryos
Bud lactobacillus.
A kind of 3. preparation method of low value fish-bone polypeptide chelate calcium according to claim 1, it is characterised in that:Described is thick
Fishbone dust step processed is:30 ~ 40wt% sodium carbonate liquors are added in the fish-bone of grinding, soak 25 ~ 35min, fish-bone and sodium carbonate
The volume ratio of solution is 1:2.5 ~ 4.5, filtering, precipitation is cleaned with pure water, is re-fed into pressure vessel, control pressure 0.14 ~
0.18MPa, 4 ~ 7min of boiling, drying and crushing obtain fishbone dust.
A kind of 4. preparation method of low value fish-bone polypeptide chelate calcium according to claim 1, it is characterised in that:Described point
It is that the homogenate of 20 ~ 30 times of volume pure water is added in fishbone dust to walk enzymolysis step, add the undaria pinnitafida protease of 5 ~ 8wt ‰ and 0.2 ~
The bioactive peptides of 0.7wt ‰, in 25 ~ 35 DEG C, 30 ~ 40min being digested under the conditions of pH7.0 ~ 9.0, regulation pH is 6.0 ~ 7.0, addition 1 ~
3wt ﹪ flavor proteases, 15 ~ 25min is digested in 52 ~ 60 DEG C, obtains enzymolysis liquid;The amino acid sequence of the bioactive peptide is
SCASRCKVSFRCAVSFCICKCFRC。
A kind of 5. preparation method of low value fish-bone polypeptide chelate calcium according to claim 1, it is characterised in that:Described etc.
For electricity point settling step for enzymolysis liquid is filtered, the pH value for adjusting filtrate is 4.5 ~ 4.8, and centrifuging and taking precipitates after standing.
A kind of 6. preparation method of low value fish-bone polypeptide chelate calcium according to claim 1, it is characterised in that:Described chela
It is by 24 ~ 48h of nanofiltration in centrifuged supernatant and pure water to close step, takes the pure water after nanofiltration, concentrates, and adds isoelectric precipitation and obtains
Fish-bone polypeptide, in 50 ~ 55 DEG C, pH chelates 1.5 ~ 2h under the conditions of being 6.8 ~ 7.2,2 ~ 4 times of absolute ethyl alcohols is added, in 25 ~ 35 DEG C
2 ~ 4h of lower standing, centrifuging and taking precipitation.
A kind of 7. preparation method of low value fish-bone polypeptide chelate calcium according to claim 1, it is characterised in that:Described is cold
Freeze in drying steps and add 20 ~ 35wt% polyethylene Topiramate Los oxazolidinone as lyophilisation additives, be freeze-dried and obtain low value fish-bone polypeptide
Chelate calcium powder.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111454347A (en) * | 2020-04-20 | 2020-07-28 | 广东海洋大学 | Peptide calcium chelate as well as preparation method and application thereof |
CN113046406A (en) * | 2021-03-11 | 2021-06-29 | 广州市金龟寿药品有限公司 | Preparation method of fishbone polypeptide |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102406176A (en) * | 2011-10-08 | 2012-04-11 | 山东荣信水产食品集团股份有限公司 | Small molecule polypeptide Ca-chelate of fishbone and preparation method |
-
2017
- 2017-07-31 CN CN201710642426.0A patent/CN107557419A/en active Pending
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102406176A (en) * | 2011-10-08 | 2012-04-11 | 山东荣信水产食品集团股份有限公司 | Small molecule polypeptide Ca-chelate of fishbone and preparation method |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111454347A (en) * | 2020-04-20 | 2020-07-28 | 广东海洋大学 | Peptide calcium chelate as well as preparation method and application thereof |
CN113046406A (en) * | 2021-03-11 | 2021-06-29 | 广州市金龟寿药品有限公司 | Preparation method of fishbone polypeptide |
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Application publication date: 20180109 |