CN102084994B - Protamine and preparation method thereof - Google Patents
Protamine and preparation method thereof Download PDFInfo
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- CN102084994B CN102084994B CN2009102204515A CN200910220451A CN102084994B CN 102084994 B CN102084994 B CN 102084994B CN 2009102204515 A CN2009102204515 A CN 2009102204515A CN 200910220451 A CN200910220451 A CN 200910220451A CN 102084994 B CN102084994 B CN 102084994B
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Abstract
The invention relates to further processing of aquatic products, specially to a protamine and a preparation method thereof. The protamine comprises, in percentage by weight, 0.75-1% of VE, 0.65-0.8% of VC, 0.7-1% of taurine, 1-3% of yeast zinc, 0.6-1% of rehmannia glutinosa extract, 0.3-0.6% of cistanche extract, 0.2-0.6% of wolfberry extract, 0.6-1% of Radix Astragali extract, 10-14% of Apostichopus japonicus enzyme-hydrolyzed powder, 20-29% of xylo oligosaccharide, 20-40% of soybean protein isolate, with the balance being protamine powder. The protamine in the invention possesses abundant nutrition, and is nutrition supplement food for male reproductive system.
Description
Technical field
The present invention relates to the aquatic products deep processing, specifically a kind of protamine and preparation method thereof.
Background technology
In science day by day flourishing today, many problems have appearred in adult male aspect sexual life, aspect healthy reproduction.People's attempt addresses this problem by modes such as medicine, physiotherapys.But the ED incidence has rejuvenation in recent years still more than 50%, the trend of extension.Left and right sides male sexual dysfunction person incidence was very high in 30 years old.All tcm health preserving methods, Traditional chinese medicine medicament produces effects not satisfactory.Though other doctor trained in Western medicine effect is good, can produce dependence, and very large side effect is arranged.Otherwise effect is not ideal enough.In all food, there is a kind of food to have good nutritive validity, that is exactly the protamine that is extracted from Pacific salmon, catfish seminal vesicle, the cell of edible this food energy vegetative reproduction system muscle, nerve, blood vessel, sufficient nutriment basis is arranged, thereby sperm, seminal fluid, the histiocytic growth of reproductive system upgrade and are protected in metabolism.The reproductive system function be can improve, fertility and sexuality improved.
Summary of the invention
The object of the present invention is to provide a kind of nutritious protamine and preparation method thereof, it is best male reproductive system nutrient supplement food.
For achieving the above object, the technical solution used in the present invention is:
A kind of protamine; by weight percentage; it consists of; VE 0.75-1%, VC0.65-0.8%, taurine 0.7-1%, yeast zinc 1-3%, cultivated land extract 0.6-1%, cistanche extracts 0.3-0.6%, Fructus lycii P.E 0.2-0.6%, Astragalus Root P.E 0.6-1%, stichopus japonicus enzymolysis powder 10-14%, xylo-oligosaccharide 20-29%, soybean protein isolate 20-40%, surplus is the protamine powder.
The preparation of described protamine:
1) preparation process of stichopus japonicus enzymolysis powder is as follows: with extra large stichopus japonicus, with seawater or water soaking 12-24 hour, clean, be ground into diameter and stick with paste less than the sea cucumber of 5mm, the enzymolysis pot of packing into, the neutral proteinase of adding sea cucumber weight 1-2 ‰, 0.5-1 ‰ food flavor enzyme, under 45-55 ℃ environment, decomposed 2-5 hour, sea cucumber hydrolysate is taken the dish out of the pot, with 100-150 mesh sieve filter cleaner, be concentrated into proportion and reach 1.05-1.10 and namely make enzymolysis sea cucumber liquid; Enzymolysis sea cucumber liquid is carried out spray-drying, stichopus japonicus enzymolysis powder;
2) A. cleaned arrangement with bright salmon and/or catfish seminal vesicle, with sodium citrate solution immersion, the cleaning of 0.1-0.3 mole, 3-4 time, each 0.5 hour;
B. with the detailed catalogue pulverizer it is pulverized pulping, inject the 0.5-1.5 molar concentration sodium chloride solution of 5-20 times of weight of slurries, extracted 0.5-3 hour; Behind the filter and remove residue, with filtrate 1-4 times of weight, volumetric concentration 90-95% ethanol precipitation 0.5-3 hour; Use centrifuge dewatering, obtain just body of protamine filament shape;
C. after just the body scissors shreds, operating process by above-mentioned B, with the 0.5-1.5 molar concentration sodium chloride solution of first body 5-20 times weight and just body 1-4 times weight, volumetric concentration 90-95% ethanol precipitation 0.5-3 hour is again refining, after centrifugal the anhydrating, can obtain highly purified protamine raw material; Soaked 6-18 hour with isopropyl alcohol, the oven dry crushing screening becomes the protamine powder again;
3) by weight percentage, in the protamine powder, add: VE 0.75-1%, VC0.65-0.8%, taurine 0.7-1%, yeast zinc 1-3%, cultivated land extract 0.6-1%, cistanche extracts 0.3-0.6%, Fructus lycii P.E 0.2-0.6%, Astragalus Root P.E 0.6-1%, stichopus japonicus enzymolysis powder 10-14%, xylo-oligosaccharide 20-29%, soybean protein isolate 20-40%; Behind the homogeneous, with ozone sterilization 20 minutes, incapsulate and get final product.
Protamine is Pacific salmon seminal vesicle extract, and clupea pallasi, salmon are the main sources of protamine.Wherein salmon claims again salmon, dog salmon.Hatching is arranged at fresh water, be grown in the ocean, the characteristics of fresh water breeding are returned in ripe migration.In adult fish migration process, go through many hardships especially, its endurance, physical efficiency are subject to baptism, and vitality is extremely strong.Just containing high-performance material and gene so in its milter seminal vesicle, is the good food that improves human body physical agility, endurance.
The protamine Applications in food.In the protamine finished product, add VE, VC, Taurine, zinc lactate, Chinese medicine cultivated land, saline cistanche, the fruit of Chinese wolfberry that the human reproductive system needs, the Radix Astragali is made desirable male reproductive system nutrient supplement food.
Protamine also has good nourishing the liver, protects the effect of liver, improves the effect of immunologic function, improves the effect of antifatigue, increase physical efficiency, the effect that eliminate free radical, delays senility.
The specific embodiment
1) selects fresh and alive distant stichopus japonicus, use sea water immersion, defecation 12 hours.Clean, being ground into diameter sticks with paste less than the sea cucumber of 5mm, the enzymolysis pot of packing into, the neutral proteinase (buy in: jiangsu wuxi city snow plum enzyme preparation Science and Technology Ltd. produces) that adds sea cucumber weight 1 ‰, 0.5 ‰ food flavor enzyme (buy in: jiangsu wuxi city snow plum enzyme preparation Science and Technology Ltd. and produce), under 50 ℃ environment, decomposed 3 hours, sea cucumber hydrolysate is taken the dish out of the pot, with 100 mesh sieve filter cleaners, again the ozone machine escape pipe is inserted in the enzymolysis liquid, with content (30-50 gram/cubic meter) ozone sterilization 15 minutes, entered low temperature (70-75 ℃) concentrated 2 hours, proportion reaches 1.10 and namely makes enzymolysis sea cucumber liquid; Enzymolysis sea cucumber liquid is carried out spray-drying, 200 order stichopus japonicus enzymolysis powder.
2) select bright salmon, catfish seminal vesicle, with natrium citricum immersion, the cleaning of 0.1 molar concentration, 3-4 time, each 0.5 hour.Then break into slurry with the detailed catalogue pulverizer, inject 1 molar concentration sodium chloride solution of 10 times of weight of slurries, extracted 1 hour.Behind the filter and remove residue, with filtrate 2 times of weight, volumetric concentration 95% ethanol precipitation 1 hour.Anhydrate with centrifuge, obtain just body of protamine filament shape.
After just the body scissors shreds, press the aforesaid operations process, again refining with 1 molar concentration sodium chloride solution and volumetric concentration 95% ethanol, after centrifugal the anhydrating, can obtain highly purified protamine raw material.Soaked 12 hours with isopropyl alcohol, 100 mesh sieves were pulverized in oven dry, became the protamine powder.
By weight percentage, in the protamine powder, add:
VE?0.75-1％、
VC?0.65-0.8％、
Taurine 0.7-1%,
Yeast zinc 1-3%,
Cultivated land extract 0.6-1%,
Cistanche extracts 0.3-0.6%,
Fructus lycii P.E 0.2-0.6%,
Astragalus Root P.E 0.6-1%,
Stichopus japonicus enzymolysis powder 10-14%,
Xylo-oligosaccharide 20-29%,
Soybean protein isolate 20-40%, surplus is the protamine powder; Behind the homogeneous, with ozone sterilization 20 minutes, incapsulate and get final product.
Wherein: cultivated land extract, cistanche extracts, Fructus lycii P.E, Astragalus Root P.E, the product that all adopts Jilin Hongjiu Biotech Co., Ltd. to produce.Xylo-oligosaccharide is purchased from pool, Guangzhou Yu trade Co., Ltd and produces; Soybean protein isolate is purchased from Shandong Guanhua Protein Co., Ltd..
Claims (2)
1. protamine, it is characterized in that: by weight percentage, it consists of,
V E 0.75-1%, V C 0.65-0.8%, taurine 0.7-1%, yeast zinc 1-3%, cultivated land extract 0.6-1%, cistanche extracts 0.3-0.6%, Fructus lycii P.E 0.2-0.6%, Astragalus Root P.E 0.6-1%, stichopus japonicus enzymolysis powder 10-14%, xylo-oligosaccharide 20-29%, soybean protein isolate 20-40%, surplus is the protamine powder;
The preparation process of wherein said stichopus japonicus enzymolysis powder is as follows: with extra large stichopus japonicus, with seawater or water soaking 12-24 hour, clean, be ground into diameter and stick with paste less than the sea cucumber of 5mm, the enzymolysis pot of packing into, the neutral proteinase of adding sea cucumber weight 1-2 ‰, 0.5-1 ‰ food flavor enzyme, under 45-55 ℃ environment, decomposed 2-5 hour, sea cucumber hydrolysate is taken the dish out of the pot, with 100-150 mesh sieve filter cleaner, be concentrated into proportion and reach 1.05-1.10 and namely make enzymolysis sea cucumber liquid; Enzymolysis sea cucumber liquid is carried out spray-drying, stichopus japonicus enzymolysis powder;
The preparation process of wherein said protamine powder is as follows:
A. bright salmon and/or catfish seminal vesicle are cleaned arrangement, with sodium citrate solution immersion, the cleaning of 0.1-0.3 mole, 3-4 time, each 0.5 hour;
B. with the detailed catalogue pulverizer it is pulverized pulping, inject the 0.5-1.5 molar concentration sodium chloride solution of 5-20 times of weight of slurries, extracted 0.5-3 hour; Behind the filter and remove residue, with filtrate 1-4 times of weight, volumetric concentration 90-95% ethanol precipitation 0.5-3 hour; Use centrifuge dewatering, obtain just body of protamine filament shape;
C. after just the body scissors shreds, operating process by above-mentioned B, with the 0.5-1.5 molar concentration sodium chloride solution of first body 5-20 times weight and just body 1-4 times weight, volumetric concentration 90-95% ethanol precipitation 0.5-3 hour is again refining, after centrifugal the anhydrating, namely obtains highly purified protamine raw material; Soaked 6-18 hour with isopropyl alcohol, the oven dry crushing screening namely gets the protamine powder again.
2. the preparation method of the described protamine of claim 1 is characterized in that:
1) preparation process of stichopus japonicus enzymolysis powder is as follows: with extra large stichopus japonicus, with seawater or water soaking 12-24 hour, clean, be ground into diameter and stick with paste less than the sea cucumber of 5mm, the enzymolysis pot of packing into, the neutral proteinase of adding sea cucumber weight 1-2 ‰, 0.5-1 ‰ food flavor enzyme, under 45-55 ℃ environment, decomposed 2-5 hour, sea cucumber hydrolysate is taken the dish out of the pot, with 100-150 mesh sieve filter cleaner, be concentrated into proportion and reach 1.05-1.10 and namely make enzymolysis sea cucumber liquid; Enzymolysis sea cucumber liquid is carried out spray-drying, stichopus japonicus enzymolysis powder;
2) A. cleaned arrangement with bright salmon and/or catfish seminal vesicle, with sodium citrate solution immersion, the cleaning of 0.1-0.3 mole, 3-4 time, each 0.5 hour;
B. with the detailed catalogue pulverizer it is pulverized pulping, inject the 0.5-1.5 molar concentration sodium chloride solution of 5-20 times of weight of slurries, extracted 0.5-3 hour; Behind the filter and remove residue, with filtrate 1-4 times of weight, volumetric concentration 90-95% ethanol precipitation 0.5-3 hour; Use centrifuge dewatering, obtain just body of protamine filament shape;
C. after just the body scissors shreds, operating process by above-mentioned B, with the 0.5-1.5 molar concentration sodium chloride solution of first body 5-20 times weight and just body 1-4 times weight, volumetric concentration 90-95% ethanol precipitation 0.5-3 hour is again refining, after centrifugal the anhydrating, namely obtains highly purified protamine raw material; Soaked 6-18 hour with isopropyl alcohol, the oven dry crushing screening namely gets the protamine powder again;
3) by weight percentage, in the protamine powder, add: V E 0.75-1%, V C0.65-0.8%, taurine 0.7-1%, yeast zinc 1-3%, cultivated land extract 0.6-1%, cistanche extracts 0.3-0.6%, Fructus lycii P.E 0.2-0.6%, Astragalus Root P.E 0.6-1%, stichopus japonicus enzymolysis powder 10-14%, xylo-oligosaccharide 20-29%, soybean protein isolate 20-40%; Behind the homogeneous, use ozone sterilization 20 minutes, namely get product.
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| CN102084994B true CN102084994B (en) | 2013-04-10 |
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| CN103735782B (en) * | 2013-12-06 | 2017-11-10 | 山东好当家海洋发展股份有限公司 | A kind of sea cucumber intestine extract composite soft capsules and preparation method thereof |
| CN107087797A (en) * | 2017-05-27 | 2017-08-25 | 舟山达康科技有限公司 | A kind of processing method of the plain product of tuna milt |
| CN115067447A (en) * | 2021-03-16 | 2022-09-20 | 王明传 | Protamine beverage and preparation method thereof |
| KR102545132B1 (en) * | 2022-05-07 | 2023-06-21 | 정원근 | Nucleoprotein powder prepared from salmon sperm and functional drinks comprising the same |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1458263A (en) * | 2002-05-15 | 2003-11-26 | 谭攸恒 | Sea cucumber nutritive wine and its producing method and use |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1458263A (en) * | 2002-05-15 | 2003-11-26 | 谭攸恒 | Sea cucumber nutritive wine and its producing method and use |
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Effective date of registration: 20180820 Address after: 116600 No. 30 Fuquan North Road, central industrial area, Dalian economic and Technological Development Zone, Liaoning Patentee after: Dalian Shen Qi aquatic products Co., Ltd. Address before: 118300 Liaoning Donggang new town Taoyuan Committee Donggang Hui Hai Marine Biotechnology Development Co., Ltd. Patentee before: Donggang City Huihai Marine Biotechnology Development Co., Ltd. |
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