CN102406176A - Small molecule polypeptide Ca-chelate of fishbone and preparation method - Google Patents
Small molecule polypeptide Ca-chelate of fishbone and preparation method Download PDFInfo
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Abstract
The invention discloses small molecule polypeptide Ca-chelate of fishbone, which belongs to the field of functional food or additive. The method for preparing the small molecule polypeptide Ca-chelate of the fishbone comprises the following steps: boiling the fishbone at high temperature and high pressure; pulping to obtain the fishbone paste; utilizing the compound protease to execute enzymolysis; centrifuging to obtain the liquid supernatant and the sediment; filtering the liquid supernatant to obtain the small molecule polypeptide liquid of the fishbone; executing the composite acid acidification through citric acid and lactic acid for the sediment so as to obtain the fishbone calcium liquid; mixing the small molecule polypeptide liquid of the fishbone with the fishbone calcium liquid; executing the chelation at 40-50 degrees centigrade while the pH value is 7.5-8.5; and drying to obtain the small molecule polypeptide Ca-chelate of the fishbone. The invention has high Ca-chelate rate which reaches over 90%, and has high biological absorption rate of the calcium which reaches over 70%. The invention has no side effects, has good flavor, can be directly absorbed from intestinal mucosa after being eaten, solves the problems that the solubility of the traditional calcium source is bad, the absorption rate is low and the side effect is large, and solves the problem of producing calculi. In addition, the invention has a simple preparation method and low cost.
Description
Technical field
The invention belongs to nutraceutical or functional food processing technique field, be specifically related to a kind of fish-bone micromolecule polypeptide chelating calcium powder, and its preparation method.
Background technology
Calcium content in the fish-bone is abundant, and is similar with the composition of skeleton calcium, is a kind of good natural calcium source.But the calcium in the fish-bone mainly is to exist with the hydroxyapatite form, is unfavorable for absorption of human body.The dissolution rate of taking the biological or chemical method to improve calcium in the fish-bone is the approach commonly used that increases the utilization rate of calcium.Main at present employing enzymolysis or acid treatment methods such as (acetic acid, citric acid, malic acid, lactic acid) prepare the fish-bone activated calcium powder, can improve the dissolution rate of calcium, help absorption of human body.
Polypeptide be molecular weight that protein obtains behind proteasome degradation be about 2～10kD have an active biologically active peptide of special physiological.(CN101731666A) disclose like patent " a kind of fishbone bioactive polypeptide calcium powder and preparation method " fish-bone polypeptide powder and the direct fishbone bioactive polypeptide calcium powder that obtains that mixes of solubility calcium; But do not carry out chelating; Therefore, the assimilation effect of calcium and range of application receive bigger restriction in its product.
Through retrieval, do not find relevant fish-bone micromolecule polypeptide and fish-bone calcium report through the fish-bone micromolecule polypeptide chelating calcium powder of chelation generation.
Summary of the invention
The object of the invention is to provide a kind of fish-bone micromolecule polypeptide chelating calcium powder.
Another purpose of the present invention is to provide the preparation method of above-mentioned fish-bone micromolecule polypeptide chelating calcium powder.
A the present invention also purpose is to provide above-mentioned fish-bone micromolecule polypeptide chelating calcium powder as food additives or the directly edible purposes of nutraceutical.
For realizing above-mentioned purpose, technical scheme of the present invention is following:
A kind of fish-bone micromolecule polypeptide of the present invention chelating calcium powder prepares according to following method:
(1) freezing fish-bone is thawed, remove the fish-bone remained on surface the flesh of fish, clean up; At pressure is that 0.145～0.165Mpa, temperature are boiling fish-bone 15～20min under 110～120 ℃ of conditions, with tissue mashing machine fish-bone is pulled an oar again, through cooling off, draining, obtains fish-bone mud; Freeze to-5～-10 ℃ of preservations;
(2) according to mass ratio between water and the fish-bone mud be 1.5～2.5: 1 ratio, in the freezing fish-bone mud of step (1) gained, add water thawing, stir 3～6min then, the fish-bone slurry; According to mass ratio between compound protease and the fish-bone slurry is that 1: 450～500 ratio adds compound protease in the fish-bone slurry, carries out enzyme digestion reaction 10～30min under 6.5～7.5 conditions at 50～70 ℃, pH then; Be incubated 3～5min down at 90～95 ℃ again, follow centrifugal 3～5min under room temperature, 4000～6000g condition, degrease is got supernatant and sediment respectively;
(3) use molecular weight to be the supernatant of filter membrane ultrafiltration step (2) gained of 3ku 2～3 times, collect filtrating, concentrate, obtain fish-bone micromolecule polypeptide liquid;
(4) the sediment water with step (2) gained cleans, dries, pulverizes, and gets fishbone dust; According to 1g between fishbone dust and the composite acid-soluble liquid: 5～10mL w/v places compound acid to carry out acidification 30～60min fishbone dust, obtains fish-bone calcium liquid; The concentration of said composite acid-soluble liquid is 10～20%;
(5) step (3) gained fish-bone micromolecule polypeptide liquid and step (4) gained fish-bone calcium liquid are mixed in stir 3～5min in the bioreactor; Then at 40～50 ℃; The pH value is 7.5～8.5 times; In bioreactor, stir chelatropic reaction 20～40min, drying gets fish-bone micromolecule polypeptide chelating calcium powder.
Fish-bone described in the above-mentioned fish-bone micromolecule polypeptide chelating calcium powder step (1) is the sole fish-bone.
Compound protease described in the above-mentioned fish-bone micromolecule polypeptide chelating calcium powder step (2) is made up of flavor protease and papain, and wherein the mass ratio between flavor protease and the papain is 1: 1.
The composite acid-soluble liquid described in the step (4) is made up of citric acid and lactic acid in the above-mentioned fish-bone micromolecule polypeptide chelating calcium powder, and the volume ratio between citric acid and the lactic acid is 1: 1; The concentration of wherein said citric acid is 10～20%; Said concentration of lactic acid is 10～20%.
Described in the above-mentioned fish-bone micromolecule polypeptide chelating calcium powder step (1) use tissue mashing machine that fish-bone is pulled an oar the time tissue mashing machine rotating speed be 12000rpm.
The preparation method of above-mentioned fish-bone micromolecule polypeptide chelating calcium powder comprises the steps:
(1) freezing fish-bone is thawed, remove the fish-bone remained on surface the flesh of fish, clean up; At pressure is that 0.145～0.165Mpa, temperature are boiling fish-bone 15～20min under 110～120 ℃ of conditions, with tissue mashing machine fish-bone is pulled an oar again, through cooling off, draining, obtains fish-bone mud again; Freeze to-5～-10 ℃ of preservations;
(2) according to mass ratio between water and the fish-bone mud be 1.5～2.5: 1 ratio, in the freezing fish-bone mud of step (1) gained, add water thawing, stir 3～6min, the fish-bone slurry; According to mass ratio between compound protease and the fish-bone slurry is that 1: 450～500 ratio adds compound protease in the fish-bone slurry, carries out enzyme digestion reaction 10～30min under 6.5～7.5 conditions at 50～70 ℃, pH then; Be incubated 3-5min down at 90～95 ℃ again, follow centrifugal 3～5min under room temperature, 4000～6000g condition, degrease is got supernatant and sediment respectively;
(3) use molecular weight to be the supernatant of filter membrane ultrafiltration step (2) gained of 3ku 2～3 times, collect filtrating, concentrate, obtain fish-bone micromolecule polypeptide liquid;
(4) the sediment water with the centrifugal gained of step (2) cleans, dries, pulverizes, and gets fishbone dust; According to 1g between fishbone dust and the composite acid-soluble liquid: 5～10mL w/v places compound acid to carry out acidification 30～60min fishbone dust, obtains fish-bone calcium liquid; The concentration of said composite acid-soluble liquid is 10～20%;
(5) step (3) resulting fish-bone micromolecule polypeptide liquid and the resulting fish-bone calcium of step (4) liquid are mixed in stir 3～5min in the bioreactor; Then at 40～50 ℃; The pH value is 7.5～8.5 times; In bioreactor, stir chelatropic reaction 20～40min, drying gets fish-bone micromolecule polypeptide chelating calcium powder.
Fish-bone described in above-mentioned preparation method's step (1) is the sole fish-bone.
Compound protease described in above-mentioned preparation method's step (2) is made up of flavor protease and papain, and the mass ratio between them is 1: 1.
Composite acid-soluble liquid described in above-mentioned preparation method's step (4) is made up of citric acid and lactic acid, and the volume ratio between them is 1: 1; The concentration of wherein said citric acid is 10～20%; Said concentration of lactic acid is 10～20%.
Described in above-mentioned preparation method's step (1) use tissue mashing machine that fish-bone is pulled an oar the time tissue mashing machine rotating speed be 12000rpm.
Advantage that the present invention has and beneficial effect: the calcium chelation percent of (1), fish-bone micromolecule polypeptide chelating calcium powder of the present invention is high, can reach more than 90%; Secondly, the biological absorption of calcium is high, can reach more than 70%.(2), fish-bone micromolecule polypeptide chelating calcium powder of the present invention has no side effect, product has light frankincense flavor, specifically local flavor preferably.(3), preparation method of the present invention is simple and easy to do, cost is low.(4), the prepared fish-bone micromolecule polypeptide chelating calcium powder of the present invention directly absorbs from intestinal mucosa with the integral form of polypeptide; Thereby exempt from some chemical factors; Like the influence of pH value, lipid, fiber, oxalic acid, phytic acid, phosphate etc., efficiently solve problems such as traditional calcium source is poorly soluble, absorptivity is low, side effect is big, generation calculus.(5), compare, fish-bone micromolecule polypeptide chelating calcium powder of the present invention can satisfy the needs of life entity to calcium constituent, can reach the double effects that replenishes polypeptide again with traditional calcium-supplementing preparation such as calcium phosphate, calcium carbonate, calcium gluconae, calcium acetate, calcium lactate etc.
The specific embodiment
The preparation of embodiment 1 fish-bone micromolecule polypeptide chelating calcium powder
(1) getting sole bone 1000 grams, through the flesh of fish, the cleaning of thawing, going remained on surface, is that 0.165Mpa, temperature are boiling fish-bone 20min under 120 ℃ of conditions at pressure; Pull an oar down at 12000 rev/mins with tissue mashing machine again, cool off, drain, obtain 380 gram fish-bone mud; Freeze to-10 ℃, subsequent use;
(2) in freezing fish-bone mud, adding entry 760mL thaws; Stirred then 5 minutes, and added compound protease (flavor protease and papain with mass ratio mix at 1: 1) 2.5 grams, carry out enzyme digestion reaction 20min under 7.0 conditions at 60 ℃, pH; Be incubated 3 minutes enzymes that go out down at 95 ℃ again; Be cooled to the centrifugal 4min of 6000g after the room temperature, degrease obtains supernatant and sediment.
(3) be the filter membrane ultrafiltration 2 times of 3ku to the supernatant of step (2) gained through molecular weight, obtain fish-bone micromolecule polypeptide liquid 450ml.
(4) centrifugal sediment to step (2) gained cleans, dries, pulverizes; Get fishbone dust; (fishbone dust and 12% acid solution obtain fish-bone calcium liquid 150ml with 1g: 10ml (w/v) processing 40min to fishbone dust through compound acid (citric acid and ammonium lacate mix according to 1: 1 volume ratio) acidifying.
(5) step (3) and step (4) resulting fish-bone micromolecule polypeptide liquid and fish-bone calcium liquid were mixed in bioreactor 5 minutes; Then at 50 ℃; The pH value is under 8.0 conditions; In bioreactor, stir chelatropic reaction 40min, pass through freeze drying (FD-1PF, development in science and technology Co., Ltd is helped in moral sky, Beijing) again and obtain fish-bone micromolecule polypeptide chelating calcium powder 62 grams.Through measuring, the calcium chelation percent is 96%.
The computational methods of calcium chelation percent:
W in the formula (1)
1: the content (mg) of calcium in the fish-bone calcium liquid before the chelatropic reaction, W
2: not chelated calcium content (mg) in the fish-bone micromolecule polypeptide chelating calcium powder.
The calcium content assay method:
(1) reagent configuration: 1.25mol/L potassium hydroxide solution: accurately take by weighing 70.13g potassium hydroxide, be diluted with water to 1000Ml.0.05mol/L sodium citrate solution: take by weighing 14.7g natrium citricum (Na
3C
6H
72H
2O), be diluted with water to 1000mL.EDTA solution: accurately take by weighing 4.50g EDTA (disodium ethylene diamine tetraacetate), be diluted with water to 1000mL, be stored in the polyethylene bottle, 4 ℃ of preservations.Dilution gets final product for 10 times during use.Calcium standard solution: accurately take by weighing 0.1248g calcium carbonate (purity is greater than 99.99%, 105 ℃～110 ℃ oven dry 2h), add 20ml water and 3mL; 0.5mol/L. dissolving with hydrochloric acid moves in the 500ml volumetric flask, thin up is to scale; Be stored in the polyethylene bottle 4 ℃ of preservations.This solution is equivalent to 100 μ g calcium for every milliliter.Calred indicator: take by weighing 0.1g calred indicator (C
21O
7N
2SH
14), being diluted with water to 100ml, can use after the dissolving.Be stored in the refrigerator and can keep more than the one and a half months.
(2) demarcate EDTA concentration: draw the 0.5mL calcium standard solution,, demarcate the concentration of its EDTA, calculate milligram number, the i.e. titer (T) that every milliliter of EDTA is equivalent to calcium according to titration results with the EDTA titration.
(3) sample and blank titration: draw respectively the 0.1mL sample in blank in test tube; Add the 0.1mL sodium citrate solution, add 1.5ML 1.25mol/L potassium hydroxide solution, add 3 calred indicator with buret; Immediately diluting the titration of 10 times of EDTA solution, become basket by aubergine to indicator till.
(4) computational methods:
W in the formula (2): calcium content in the sample, unit are milligram every hectogram (mg/100g); T:EDTA titer, unit is every milliliter (mg/mL) of milligram; V: used EDTA amount during the titration sample, unit is a milliliter (mL); V
0: used EDTA amount when titration is blank, unit is a milliliter (mL); F: sample extension rate; M: sample mass, unit is gram (g).
The infrared spectrum test chelatropic reaction: behind polypeptide and the calcium ion generation chelatropic reaction, the waveform of its infrared spectrum has change to a certain degree before and after reaction, can change through it and identify whether polypeptide and calcium ion chelatropic reaction has taken place.Through getting the pressed powder of polypeptide powder and fish-bone micromolecule polypeptide chelating calcium powder, adopt pellet technique to carry out infrared spectrum measurement.
The preparation of embodiment 2 fish-bone micromolecule polypeptide chelating calcium powders
(1) get sole bone 2000 gram, through the flesh of fish, cleaning, the high pressure 0.165Mpa pressure that thaws, goes remained on surface, 120 ℃ of boiling fish-bone 15min, 12000 rev/mins of making beating down of tissue mashing machine are cooled off, drained and obtain 780 gram fish-bone mud, freeze to-10 ℃, and are subsequent use;
(2) in freezing fish-bone mud, adding entry 1250mL thaws; Stirred then 5 minutes, and added compound protease (flavor protease and papain with mass ratio mix at 1: 1) 4.5 grams, carry out enzyme digestion reaction 30min under 7.0 conditions at 60 ℃, pH; Again at 95 ℃ of following insulation 3min enzymes that go out; Be cooled to the centrifugal 5min of 6000g after the room temperature, degrease obtains supernatant and sediment.
(3) be the filter membrane ultrafiltration 2 times of 3ku to the supernatant of step (2) gained through molecular weight, obtain fish-bone micromolecule polypeptide filtered fluid 800ml.
(4) to the centrifugal sediment of step (2) gained; Obtain fishbone dust through cleaning, oven dry, pulverization process; (fishbone dust and 12% composite acid-soluble liquid obtain fish-bone calcium liquid 250ml with 1g: 8ml (w/v) processing 50min to fishbone dust through compound acid (citric acid and lactic acid mix at 1: 1) acidifying.
(5) step (3) and step (4) resulting fish-bone micromolecule polypeptide liquid and fish-bone calcium liquid are mixed 5min in bioreactor; Then at 48 ℃; The pH value is under 8.0 conditions, in bioreactor, stirs chelatropic reaction 30min, passes through freeze drying (FD-1PF again; Development in science and technology Co., Ltd is helped in moral sky, Beijing) 24 hours, obtain fish-bone micromolecule polypeptide chelating calcium powder 125 grams.
Detect according to method described in the embodiment 1, the calcium chelation percent is 96.5%.
The preparation of embodiment 3 fish-bone micromolecule polypeptide chelating calcium powders
(1) getting sole bone 1500 grams, through the flesh of fish, the cleaning of thawing, going remained on surface, is 0.160Mpa pressure at pressure; 115 ℃ of following boiling fish-bone 20min pull an oar under 12000rpm with tissue mashing machine, cool off, drain; Obtain 580 gram fish-bone mud, freeze to-10 ℃;
(2) in freezing fish-bone mud, add entry 1050mL and thaw, stir 4min then, add compound protease (flavor protease and papain mix with mass ratio at 1: 1) 3.3 grams.Advance enzyme digestion reaction 25min under 7.2 conditions at 55 ℃, pH then, again at 95 ℃ of 3 minutes enzymes that go out of insulation down, be cooled to the centrifugal 5min of 6000g after the room temperature, degrease obtains supernatant and sediment.
(3) be the filter membrane ultrafiltration 2 times of 3ku to the supernatant of step (2) gained through molecular weight, obtain fish-bone micromolecule polypeptide filtered fluid 650ml.
(4) to the centrifugal sediment of step (2) gained; Obtain fishbone dust through cleaning, oven dry, pulverization process; Fishbone dust through compound acid (citric acid and lactic acid mix at 1: 1) acidifying (fishbone dust and 10% composite acid-soluble liquid with 1g: 9ml (w/v) processing 30min after, obtain fish-bone calcium liquid 220ml.
(5) step (3) and step (4) resulting fish-bone micromolecule polypeptide liquid and fish-bone calcium liquid are mixed 5min in bioreactor; Then at 45 ℃; The pH value is under 8.2 conditions; In bioreactor, stir chelatropic reaction 20min, pass through freeze drying (FD-1PF, development in science and technology Co., Ltd is helped in moral sky, Beijing) again and obtain fish-bone micromolecule polypeptide chelating calcium powder 93.6 grams.
Detect according to method described in the embodiment 1, the calcium chelation percent is 98%.
The determination test of calcium biological absorption in the embodiment 4 fish-bone micromolecule polypeptide chelating calcium powders
Carry out according to following method:
Get experiment Wistar rat (animal medicine institute of China Agricultural University provides) 50, every body weight 180～200g, basal feed adaptability fed for 1 week, was divided into 5 groups then at random:
A group: basal feed (basal feed constituent and percentage by weight thereof: casein 20%, cornstarch 65%, corn oil 8%, cellulose 1%, B B-complex 1%, complex inorganic salt catalyst 5%);
B group: the fish-bone micromolecule polypeptide chelating calcium powder 5g/ (kgd) of basal feed+embodiment 1;
C group: basal feed+fishbone dust (not chelating) 5g/ (kgd);
The calcium content actual measurement is 1159mg/kg in the basal feed.
Each is organized rat and under equal conditions raises, and freely ingests, and weighs weekly 1 time, writes down food-intake every day.The 4th week back immigration metabolic cage, excrement, urine are collected in the calcium metabolism experiment of carrying out 3 days.
The calcium absorptivity computing formula:
Calcium absorptivity (%)=(calcium intake one excrement calcium discharge rate)/calcium intake * 100%.
Experimental result such as following table
Table 1 calcium absorptivity comparative test result
| Test is divided into groups | Calcium absorptivity (%) |
| The A group | 26.6±1.08 |
| The B group | 71.18±3.78 |
| The C group | 51.07±3.23 |
The result shows that fish-bone micromolecule polypeptide chelating calcium powder absorptivity of the present invention reaches more than 70%, is significantly higher than the absorptivity (51%) of calcium in the fishbone dust.
Claims (8)
1. fish-bone micromolecule polypeptide chelating calcium powder is characterized in that according to following method preparation:
(1) freezing fish-bone is thawed, remove the fish-bone remained on surface the flesh of fish, clean up; At pressure is that 0.145～0.165Mpa, temperature are boiling fish-bone 15～20min under 110～120 ℃ of conditions, with tissue mashing machine fish-bone is pulled an oar again, through cooling off, draining, obtains fish-bone mud; Freeze to-5～-10 ℃ of preservations;
(2) according to mass ratio between water and the fish-bone mud be 1.5～2.5: 1 ratio, in the freezing fish-bone mud of step (1) gained, add water thawing, stir 3～6min then, the fish-bone slurry; According to mass ratio between compound protease and the fish-bone slurry is that 1: 450～500 ratio adds compound protease in the fish-bone slurry, carries out enzyme digestion reaction 10～30min under 6.5～7.5 conditions at 50～70 ℃, pH then; Be incubated 3～5min down at 90～95 ℃ again, follow centrifugal 3～5min under room temperature, 4000～6000g condition, degrease is got supernatant and sediment respectively; Described compound protease is made up of flavor protease and papain;
(3) use molecular weight to be the supernatant of filter membrane ultrafiltration step (2) gained of 3ku 2～3 times, collect filtrating, concentrate, obtain fish-bone micromolecule polypeptide liquid;
(4) the sediment water with step (2) gained cleans, dries, pulverizes, and gets fishbone dust; According to 1g between fishbone dust and the composite acid-soluble liquid: 5～10mL w/v places compound acid to carry out acidification 30～60min fishbone dust, obtains fish-bone calcium liquid; The concentration of said composite acid-soluble liquid is 10～20%; Described composite acid-soluble liquid is made up of citric acid and lactic acid;
(5) step (3) gained fish-bone micromolecule polypeptide liquid and step (4) gained fish-bone calcium liquid are mixed in stir 3～5min in the bioreactor; Then at 40～50 ℃; The pH value is 7.5～8.5 times; In bioreactor, stir chelatropic reaction 20～40min, drying gets fish-bone micromolecule polypeptide chelating calcium powder.
2. according to the described fish-bone micromolecule polypeptide of claim 1 chelating calcium powder, it is characterized in that the fish-bone described in its step (1) is the sole fish-bone.
3. according to claim 1 or 2 described fish-bone micromolecule polypeptide chelating calcium powders, it is characterized in that the mass ratio between the flavor protease and papain is 1: 1 in the compound protease described in its step (2).
4. according to the described fish-bone micromolecule polypeptide of claim 3 chelating calcium powder, it is characterized in that the volume ratio between the citric acid and lactic acid is 1: 1 in the composite acid-soluble liquid described in its step (4); The concentration of wherein said citric acid is 10～20%; Said concentration of lactic acid is 10～20%.
5. according to the described fish-bone micromolecule polypeptide of claim 3 chelating calcium powder, it is characterized in that described in its step (1) use tissue mashing machine that fish-bone is pulled an oar the time tissue mashing machine rotating speed be 12000rpm.
6. the preparation method of the described fish-bone micromolecule polypeptide of claim 1 chelating calcium powder is characterized in that comprising the steps:
(1) freezing fish-bone is thawed, remove the fish-bone remained on surface the flesh of fish, clean up; At pressure is that 0.145～0.165Mpa, temperature are boiling fish-bone 15～20min under 110～120 ℃ of conditions, with tissue mashing machine fish-bone is pulled an oar again, through cooling off, draining, obtains fish-bone mud again; Freeze to-5～-10 ℃ of preservations;
(2) according to mass ratio between water and the fish-bone mud be 1.5～2.5: 1 ratio, in the freezing fish-bone mud of step (1) gained, add water thawing, stir 3～6min, the fish-bone slurry; According to mass ratio between compound protease and the fish-bone slurry is that 1: 450～500 ratio adds compound protease in the fish-bone slurry, carries out enzyme digestion reaction 10～30min under 6.5～7.5 conditions at 50～70 ℃, pH then; Be incubated 3-5min down at 90～95 ℃ again, follow centrifugal 3～5min under room temperature, 4000～6000g condition, degrease is got supernatant and sediment respectively; Described compound protease is made up of flavor protease and papain, and the mass ratio between them is 1: 1;
(3) use molecular weight to be the supernatant of filter membrane ultrafiltration step (2) gained of 3ku 2～3 times, collect filtrating, concentrate, obtain fish-bone micromolecule polypeptide liquid;
(4) the sediment water with the centrifugal gained of step (2) cleans, dries, pulverizes, and gets fishbone dust; According to 1g between fishbone dust and the composite acid-soluble liquid: 5～10mL w/v places compound acid to carry out acidification 30～60min fishbone dust, obtains fish-bone calcium liquid; The concentration of said composite acid-soluble liquid is 10～20%; Described composite acid-soluble liquid is made up of citric acid and lactic acid, and the volume ratio between them is 1: 1; The concentration of wherein said citric acid is 10～20%; Said concentration of lactic acid is 10～20%;
(5) step (3) resulting fish-bone micromolecule polypeptide liquid and the resulting fish-bone calcium of step (4) liquid are mixed in stir 3～5min in the bioreactor; Then at 40～50 ℃; The pH value is 7.5～8.5 times; In bioreactor, stir chelatropic reaction 20～40min, drying gets fish-bone micromolecule polypeptide chelating calcium powder.
7. according to the described preparation method of claim 6, it is characterized in that the fish-bone described in the step (1) is the sole fish-bone.
8. the arbitrary described fish-bone micromolecule polypeptide chelating calcium powder of claim 1-5 is as the purposes of food additives or nutraceutical.
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